FvH4_6g35860

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003362
Physical Location & Seq
Forward (+)
28274655 .. 28280876
6222 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35860.t8

Sequence Viewer

Length: 1686 bp
ATGGTTGCTATTGGGTCTGCAGCATTGCCATTGAGAAGGTTTGAAGCTTCAATGGAGGACGATATGTCGTTTAAAAGTGGTAGGAAGAAAATGGGCAAGAGTAGAAAGCCTGAGAAGCAAGGTCAAGGTCGGTCCTTTGATGCTGGTGCTGGTAAAACTGTATCAGGGCGGTGCTCGAATGCGGCAACACAAACAGTTGTGAGGAAACAGGTTGACCCCGAGACGGCAAAGTACTTTTCGGAGATTGCTAATCTTTTCGAGAGTGAGGGGATTGATATTGAGGAGCGGTCGTCTTTATGTAGTAATGCGCTTGAGGAAACTAGGGGGAAGGAAATGGAACTTGCCACGGACTATATCATTAGTCACACTTTGCAGACTCTTGTCGAGGGATGCGACGTCGACTGTCTTTGTGCTTTTCTCCGAGGCTGTGCAAAGAATTTCCCATTGATTGCGATGGATAAGTCGGGATCTCATGTGGCTGAGTCTGCCATCAAGGCTTTGTCTAGGCATCTTCAGGAGAGCGACGCCTATGCTGTTATTGAGGACACTCTAAATACAATATGCAAGGTGATTGTAAAGAATCCTGTTGATCTGATGTGTGATTGCTACGGATCTCATGTTTTGCGAAGTCTTCTCTCTCTTTTTAAAGGAGTGCCATTTGATTCTTCTGATTTCCGAATGAGGAAGTCATCTTCTGCCTTACCAGAACGGTTTAATTTCAAGGCACCTCGATCAGATGCAGATGTTTCACAACATCTTATTCCGGGATTCCCAGATTTACTTAAGTTTCTTGTTTCCGGAATGTTAAAATGTGCTCAGAACAATGTTAAAACCCTGCAAGTTGATCAGTTCAGCAGTTTGGTTCTGCAGACTGCATTGAAGATGTTAGCAGGAGATGAAGAAATGCTGTTGCAAATAATTCCCATTCTTACTGGATGTACGAGGGAAGATATTGTAGAGGAAAACACCATTGAACCAACTATTGTGAGAGATGTTTTAGGTTTGATGAAAGAAACAGCATTTAGCCATTTAATGGAGGTTGTTTTGGAAGTAGCTCCTGATGTCTTGTACAATGGAATCTTCTTAAAGATCTTCAGGAACTCGTTATTTGACCTTTCATCCCATCATTGTGGGAATTTTGTCATCCAAGCACTTATTTCTCATGCAAAGAATCATGATCAAATGGAGATGATCTGGGAGGAACTTGGTTCGAATTTCAGGGATCTGCTTAAAATGGGAAGATCTGGTGTTATTGCTTCGCTTATGGCTGCAAGTCAAAGGCTTCATAGTTACGAACAGAAGTGTTGTGAGGCTCTTGCTGCTGCAGTACATTCAAGTAATGAGTCTTCAACATGCATTGTTCCCCGAATACTGTTTCTTGATGGCTACTTCAACTGTGAAGATAAGTCTAATTGGAATTGGCCAAGTGGTGTTAAAATGAATGTCATGGGTTCTTTGATACTGCAAGAAGTTTTTCGATTTCAAAATGAATTTATACAGCCTTTTGTAACAAGTATTACATCCATCCCAACTGATAATGTCCTCGAAGCTGCTAAGGATCCAGGAGGTGCTCGTGTTATTGAAGCATTTCTAAGTTCAAATGCTTCTGCAAAATTAAAACGCCGCTTAGTTATGAGATTTTATGCTGTATACTTTTCAGGCTGCGTGGACATTTTGGAGAGCTAG

Protein Analysis

562

Amino Acids

62.2

Weight (kDa)

5.75

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF_NOP9 PF22493 109 - 593 2e-65 NOP9-like PUF repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 399
AccB1I GGYRCC 1 cut(s) 724
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccBSI CCGCTC 1 cut(s) 286
AccI GTMKAC 2 cut(s) 399, 1650
AccIII TCCGGA 1 cut(s) 797
AciI CCGC 4 cut(s) 169, 182, 286, 1624
AclWI GGATC 5 cut(s) 475, 619, 1230, 1553, 1566
AcoI YGGCCR 1 cut(s) 1421
AcsI RAATTY 4 cut(s) 436, 1135, 1213, 1490
AcuI CTGAAG 2 cut(s) 497, 1078
AcyI GRCGYC 2 cut(s) 396, 525
AfaI GTAC 4 cut(s) 233, 940, 1070, 1329
AfiI CCNNNNNNNGG 2 cut(s) 223, 1033
AflII CTTAAG 1 cut(s) 782
AhdI GACNNNNNGTC 1 cut(s) 64
AjnI CCWGG 1 cut(s) 1561
AluBI AGCT 4 cut(s) 47, 1055, 1550, 1683
AluI AGCT 4 cut(s) 47, 1055, 1550, 1683
Alw21I GWGCWC 3 cut(s) 176, 817, 1573
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 5 cut(s) 475, 619, 1230, 1553, 1566
Ama87I CYCGRG 1 cut(s) 218
Aor13HI TCCGGA 1 cut(s) 797
AoxI GGCC 1 cut(s) 1421
ApeKI GCWGC 6 cut(s) 20, 1268, 1319, 1322, 1550, 1662
ApoI RAATTY 4 cut(s) 436, 1135, 1213, 1490
Asp700I GAANNNNTTC 2 cut(s) 1473, 1587
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 132
AsuC2I CCSGG 1 cut(s) 765
AsuHPI GGTGA 1 cut(s) 580
AsuII TTCGAA 1 cut(s) 1211
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 1 cut(s) 132
BalI TGGCCA 1 cut(s) 1423
BamHI GGATCC 1 cut(s) 1558
BanI GGYRCC 1 cut(s) 724
BarI GAAGNNNNNNTAC 2 cut(s) 1330, 1362
BauI CACGAG 1 cut(s) 1572
BbsI GAAGAC 2 cut(s) 623, 1338
Bbv12I GWGCWC 3 cut(s) 176, 817, 1573
BbvI GCAGC 6 cut(s) 32, 1255, 1306, 1309, 1537, 1649
BccI CCATC 5 cut(s) 448, 497, 1131, 1376, 1532
BceAI ACGGC 1 cut(s) 240
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BciT130I CCWGG 1 cut(s) 1563
BclI TGATCA 2 cut(s) 844, 1177
BcnI CCSGG 1 cut(s) 765
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 3 cut(s) 321, 504, 1684
BfmI CTRYAG 3 cut(s) 18, 866, 1323
BfrI CTTAAG 1 cut(s) 782
BglI GCCNNNNNGGC 1 cut(s) 494
BglII AGATCT 2 cut(s) 1089, 1241
BisI GCNGC 8 cut(s) 21, 183, 1269, 1320, 1323, 1551, 1624, 1663
BlsI GCNGC 8 cut(s) 22, 184, 1270, 1321, 1324, 1552, 1625, 1664
BmcAI AGTACT 1 cut(s) 233
Bme1390I CCNGG 2 cut(s) 765, 1563
Bme18I GGWCC 1 cut(s) 132
BmeRI GACNNNNNGTC 1 cut(s) 64
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 132
BmiI GGNNCC 2 cut(s) 726, 1560
BmrFI CCNGG 2 cut(s) 765, 1563
BmsI GCATC 4 cut(s) 130, 380, 517, 727
BoxI GACNNNNGTC 1 cut(s) 380
BpiI GAAGAC 2 cut(s) 623, 1338
Bpu10I CCTNAGC 1 cut(s) 1554
Bpu14I TTCGAA 1 cut(s) 1211
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 765
BsaHI GRCGYC 2 cut(s) 396, 525
BsaJI CCNNGG 2 cut(s) 345, 421
BsaWI WCCGGW 1 cut(s) 797
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 1033
Bse1I ACTGG 1 cut(s) 937
Bse3DI GCAATG 1 cut(s) 23
BseAI TCCGGA 1 cut(s) 797
BseBI CCWGG 1 cut(s) 1563
BseDI CCNNGG 2 cut(s) 345, 421
BseGI GGATG 6 cut(s) 395, 941, 1118, 1143, 1520, 1524
BseLI CCNNNNNNNGG 2 cut(s) 223, 1033
BseMI GCAATG 1 cut(s) 23
BseMII CTCAG 3 cut(s) 102, 471, 830
BseNI ACTGG 1 cut(s) 937
BseRI GAGGAG 1 cut(s) 296
BseXI GCAGC 6 cut(s) 32, 1255, 1306, 1309, 1537, 1649
Bsh1285I CGRYCG 1 cut(s) 290
BshFI GGCC 1 cut(s) 1423
BshNI GGYRCC 1 cut(s) 724
BsiEI CGRYCG 1 cut(s) 290
BsiHKAI GWGCWC 3 cut(s) 176, 817, 1573
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 2 cut(s) 764, 798
BslI CCNNNNNNNGG 2 cut(s) 223, 1033
BsmAI GTCTC 1 cut(s) 215
BsmBI CGTCTC 1 cut(s) 215
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 1 cut(s) 1423
BsoBI CYCGRG 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 1211
Bsp1286I GDGCHC 3 cut(s) 176, 817, 1573
Bsp13I TCCGGA 1 cut(s) 797
Bsp1407I TGTACA 1 cut(s) 1068
BspACI CCGC 4 cut(s) 169, 182, 286, 1624
BspANI GGCC 1 cut(s) 1423
BspCNI CTCAG 3 cut(s) 103, 472, 829
BspEI TCCGGA 1 cut(s) 797
BspHI TCATGA 1 cut(s) 1174
BspLI GGNNCC 2 cut(s) 726, 1560
BspMAI CTGCAG 3 cut(s) 22, 870, 1327
BspPI GGATC 5 cut(s) 475, 619, 1230, 1553, 1566
BspT104I TTCGAA 1 cut(s) 1211
BspT107I GGYRCC 1 cut(s) 724
BspTI CTTAAG 1 cut(s) 782
BsrBI CCGCTC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 23
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 1 cut(s) 937
BssECI CCNNGG 2 cut(s) 345, 421
BssNAI GTATAC 1 cut(s) 1651
BssNI GRCGYC 2 cut(s) 396, 525
BssSI CACGAG 1 cut(s) 1572
Bst1107I GTATAC 1 cut(s) 1651
Bst2BI CACGAG 1 cut(s) 1572
Bst2UI CCWGG 1 cut(s) 1563
Bst4CI ACNGT 6 cut(s) 160, 196, 404, 711, 1374, 1397
BstACI GRCGYC 2 cut(s) 396, 525
BstAFI CTTAAG 1 cut(s) 782
BstAUI TGTACA 1 cut(s) 1068
BstBI TTCGAA 1 cut(s) 1211
BstDEI CTNAG 6 cut(s) 111, 480, 816, 1554, 1592, 1627
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 6 cut(s) 395, 941, 1118, 1143, 1520, 1524
BstHHI GCGC 1 cut(s) 310
BstMAI GTCTC 1 cut(s) 215
BstMCI CGRYCG 1 cut(s) 290
BstMWI GCNNNNNNNGC 4 cut(s) 115, 485, 494, 1319
BstNI CCWGG 1 cut(s) 1563
BstNSI RCATGY 1 cut(s) 1356
BstPAI GACNNNNGTC 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 763, 1561
BstSFI CTRYAG 3 cut(s) 18, 866, 1323
BstV1I GCAGC 6 cut(s) 32, 1255, 1306, 1309, 1537, 1649
BstV2I GAAGAC 2 cut(s) 623, 1338
BstX2I RGATCY 6 cut(s) 467, 611, 1089, 1222, 1241, 1558
BstXI CCANNNNNNTGG 1 cut(s) 1130
BstYI RGATCY 6 cut(s) 467, 611, 1089, 1222, 1241, 1558
BstZ17I GTATAC 1 cut(s) 1651
BsuRI GGCC 1 cut(s) 1423
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 467
BtsCI GGATG 6 cut(s) 395, 941, 1118, 1143, 1520, 1524
CciI TCATGA 1 cut(s) 1174
CfoI GCGC 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 132
CseI GACGC 1 cut(s) 533
Csp6I GTAC 4 cut(s) 232, 939, 1069, 1328
CviAII CATG 6 cut(s) 473, 617, 1163, 1175, 1353, 1447
CviQI GTAC 4 cut(s) 232, 939, 1069, 1328
DdeI CTNAG 6 cut(s) 111, 480, 816, 1554, 1592, 1627
DraI TTTAAA 2 cut(s) 73, 646
DriI GACNNNNNGTC 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 1421
Eam1105I GACNNNNNGTC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 132
Eco57I CTGAAG 2 cut(s) 497, 1078
Eco88I CYCGRG 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 1561
EcoT22I ATGCAT 1 cut(s) 1358
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 6 cut(s) 476, 620, 1166, 1178, 1356, 1450
FatI CATG 6 cut(s) 472, 616, 1162, 1174, 1352, 1446
FbaI TGATCA 2 cut(s) 844, 1177
FblI GTMKAC 2 cut(s) 399, 1650
Fnu4HI GCNGC 8 cut(s) 21, 183, 1269, 1320, 1323, 1551, 1624, 1663
FokI GGATG 6 cut(s) 402, 948, 1105, 1130, 1507, 1511
Fsp4HI GCNGC 8 cut(s) 21, 183, 1269, 1320, 1323, 1551, 1624, 1663
FspBI CTAG 3 cut(s) 321, 504, 1684
GlaI GCGC 1 cut(s) 309
GluI GCNGC 8 cut(s) 21, 183, 1269, 1320, 1323, 1551, 1624, 1663
HaeIII GGCC 1 cut(s) 1423
HapII CCGG 2 cut(s) 764, 798
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 310
Hin1I GRCGYC 2 cut(s) 396, 525
Hin1II CATG 6 cut(s) 476, 620, 1166, 1178, 1356, 1450
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HincII GTYRAC 2 cut(s) 214, 400
HindII GTYRAC 2 cut(s) 214, 400
HindIII AAGCTT 1 cut(s) 45
HinfI GANTC 8 cut(s) 376, 482, 580, 662, 768, 1077, 1171, 1343
HpaII CCGG 2 cut(s) 764, 798
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 4 cut(s) 214, 400, 1651, 1669
Hpy188I TCNGA 7 cut(s) 241, 422, 594, 670, 677, 736, 819
Hpy188III TCNNGA 8 cut(s) 259, 465, 515, 798, 1058, 1096, 1175, 1379
Hpy8I GTNNAC 4 cut(s) 214, 400, 1651, 1669
Hpy99I CGWCG 3 cut(s) 398, 401, 527
HpyAV CCTTC 2 cut(s) 30, 322
HpyCH4III ACNGT 6 cut(s) 160, 196, 404, 711, 1374, 1397
HpyCH4IV ACGT 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 485, 494, 1319
HpyF3I CTNAG 6 cut(s) 111, 480, 816, 1554, 1592, 1627
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 2 cut(s) 396, 525
Hsp92II CATG 6 cut(s) 476, 620, 1166, 1178, 1356, 1450
HspAI GCGC 1 cut(s) 308
Kpn2I TCCGGA 1 cut(s) 797
Ksp22I TGATCA 2 cut(s) 844, 1177
LmnI GCTCC 2 cut(s) 283, 1060
Lsp1109I GCAGC 6 cut(s) 32, 1255, 1306, 1309, 1537, 1649
LweI GCATC 4 cut(s) 130, 380, 517, 727
MaeI CTAG 3 cut(s) 321, 504, 1684
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 3 cut(s) 362, 1289, 1507
MbiI CCGCTC 1 cut(s) 286
MflI RGATCY 6 cut(s) 467, 611, 1089, 1222, 1241, 1558
MhlI GDGCHC 3 cut(s) 176, 817, 1573
MlsI TGGCCA 1 cut(s) 1423
MluCI AATT 9 cut(s) 436, 715, 918, 1135, 1213, 1411, 1417, 1490, 1613
MluNI TGGCCA 1 cut(s) 1423
MlyI GAGTC 3 cut(s) 370, 491, 1352
Mox20I TGGCCA 1 cut(s) 1423
Mph1103I ATGCAT 1 cut(s) 1358
MroI TCCGGA 1 cut(s) 797
MroXI GAANNNNTTC 2 cut(s) 1473, 1587
MscI TGGCCA 1 cut(s) 1423
MslI CAYNNNNRTG 1 cut(s) 1128
Msp20I TGGCCA 1 cut(s) 1423
MspCI CTTAAG 1 cut(s) 782
MspI CCGG 2 cut(s) 764, 798
MspR9I CCNGG 2 cut(s) 765, 1563
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 1 cut(s) 1563
MwoI GCNNNNNNNGC 4 cut(s) 115, 485, 494, 1319
NciI CCSGG 1 cut(s) 765
NlaIII CATG 6 cut(s) 476, 620, 1166, 1178, 1356, 1450
NlaIV GGNNCC 2 cut(s) 726, 1560
NmuCI GTSAC 1 cut(s) 362
NsiI ATGCAT 1 cut(s) 1358
NspI RCATGY 1 cut(s) 1356
NspV TTCGAA 1 cut(s) 1211
PagI TCATGA 1 cut(s) 1174
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 1473, 1587
PfeI GAWTC 5 cut(s) 580, 662, 768, 1077, 1171
PflMI CCANNNNNTGG 1 cut(s) 1033
PfoI TCCNGGA 2 cut(s) 763, 1561
PkrI GCNGC 8 cut(s) 22, 184, 1270, 1321, 1324, 1552, 1625, 1664
PleI GAGTC 3 cut(s) 370, 490, 1351
PpsI GAGTC 3 cut(s) 370, 490, 1351
PshAI GACNNNNGTC 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 1561
PspGI CCWGG 1 cut(s) 1561
PspN4I GGNNCC 2 cut(s) 726, 1560
PspPI GGNCC 1 cut(s) 132
PstI CTGCAG 3 cut(s) 22, 870, 1327
PsuI RGATCY 6 cut(s) 467, 611, 1089, 1222, 1241, 1558
RsaI GTAC 4 cut(s) 233, 940, 1070, 1329
RsaNI GTAC 4 cut(s) 232, 939, 1069, 1328
RseI CAYNNNNRTG 1 cut(s) 1128
SalI GTCGAC 1 cut(s) 398
SatI GCNGC 8 cut(s) 21, 183, 1269, 1320, 1323, 1551, 1624, 1663
Sau96I GGNCC 1 cut(s) 132
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 3 cut(s) 370, 491, 1352
ScrFI CCNGG 2 cut(s) 765, 1563
SduI GDGCHC 3 cut(s) 176, 817, 1573
SfaNI GCATC 4 cut(s) 130, 380, 517, 727
SfcI CTRYAG 3 cut(s) 18, 866, 1323
SfuI TTCGAA 1 cut(s) 1211
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 1128
SmlI CTYRAG 2 cut(s) 311, 782
SmoI CTYRAG 2 cut(s) 311, 782
Sse9I AATT 9 cut(s) 436, 715, 918, 1135, 1213, 1411, 1417, 1490, 1613
SsiI CCGC 4 cut(s) 169, 182, 286, 1624
SspMI CTAG 3 cut(s) 321, 504, 1684
StyD4I CCNGG 2 cut(s) 763, 1561
TaaI ACNGT 6 cut(s) 160, 196, 404, 711, 1374, 1397
TaiI ACGT 1 cut(s) 399
TaqI TCGA 8 cut(s) 176, 258, 384, 399, 730, 1211, 1477, 1545
TaqII GACCGA 1 cut(s) 120
TasI AATT 9 cut(s) 436, 715, 918, 1135, 1213, 1411, 1417, 1490, 1613
TatI WGTACW 3 cut(s) 231, 1068, 1327
TauI GCSGC 2 cut(s) 185, 1626
TfiI GAWTC 5 cut(s) 580, 662, 768, 1077, 1171
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 6 cut(s) 20, 1268, 1319, 1322, 1550, 1662
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 6 cut(s) 912, 1022, 1107, 1274, 1454, 1503
TspGWI ACGGA 2 cut(s) 362, 624
Van91I CCANNNNNTGG 1 cut(s) 1033
Vha464I CTTAAG 1 cut(s) 782
VpaK11BI GGWCC 1 cut(s) 132
XapI RAATTY 4 cut(s) 436, 1135, 1213, 1490
XceI RCATGY 1 cut(s) 1356
XmiI GTMKAC 2 cut(s) 399, 1650
XmnI GAANNNNTTC 2 cut(s) 1473, 1587
XspI CTAG 3 cut(s) 321, 504, 1684
ZraI GACGTC 1 cut(s) 397
ZrmI AGTACT 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.