Rroxscaffold_2G00099210

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003362
Physical Location & Seq
Reverse (-)
20798538 .. 20808321
9784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099210.1

Sequence Viewer

Length: 3015 bp
ATGAAGTCCCGAGGCCTTGAACTAGAACACCGAGTTTATGGAATACTAATCCACCGGTCTGCTGCGTATGTCCAAAAGATGCTCGGGCTATTTAACATGGACAAAGCGCATCCTGCTAGCACTTCCATAATCGGTCGAAGTTTGGATGCAAAGACAGATCCATTTCATCCAAAGGAAGATGACGAAGAGGTGGTGCGAGCTGAAATTCCCTATCAAAGTGCAATAGACGCATTATTGTACTTAGCCCAATGTACTCAACCAAACATTGCATTCTCAATGAACTTGTTAGCTAGATTTAGCTCGGTGCCAACGCAGCGTCAACGAAATGGTATCAAGAATATCTTCCGATACCTAAAAGGAACCATTGACTTGGGACTGTTCTTTCCCTATAGAGAGACAAGAGGGACCGTAGATGGAACTGCAATCCCTAAAGGAAATGTTGATGCGAAAGCGCTACTTACTACACCGAAACCCCAAATGATGTTTTGGTTGGGCGATATCTTGGAGATCAACCAAGAAAACCCTTGTGGATACTTCCTCGAACAACTCGAGAGATCATTGCTCTATATGAGGCGCATTGCCTCAATGGAGGACGACGGGTCGTATAAACGTGGTAGGAAGAAAATGGGTATGTCTAGAAAGCCTGAAAAGCAAGGTCAAGGTCAAGGTCGGTCCTTTGATGCTGGTGTTAGTAAAACTGCATCATCGGGGCGGGGCTCGAATCGGGAGAGCTATTCGGCACCGCAGACTGTTGTGAGGTACTGCATTATGAGGAAACAGGTTGACCCCGAAACGGCAAAGTACTTTTCGGAGATTGCTAACCTTTTCGAGAGTGATGGGATTGATATTGAGGAGCGGGTCGTCTTTATGTACTGTGCGAAGAACTTCCCTTTGATTGCAATGGATAAGTCTGGATCTCATGTAGTTGAGTCTGCCATCAGGGCTTTATCTAAGCATCTTCAGGAGAGTGACGCGTATGCTGTTATTGAGGACACTCTAAATACAATATGCAAGTCTTCTCTCTCTTTTAAAGGAGTGCCGTTGGATTCTTCTGAGTTCCGAATGAGGAAGTCATCTTCTGCTCTACCAGAACGGTTTAATTTCAAGGCATCGCGTTCAGACACAGATGTTTCACCACATGTTATACCTGGATTCCCGGATTTACTGAAGTTTCTTGTTTCTGGGATGTTAAAATGTGCTCGAAACAACATTAAAACCCTGCAAGTTGATCAGTACAGCAGTTTGACTACTCTGAAGTTGTTAGCAGGGGATGAAGAAGAGCTGTTGCAAATAATTCCCATTCTTATTGGATGTACCAGGGAAGATATTGCAGAAGAGAACTCCATTGAGCCAACTATTGTGAGAGATGTTTTAGGTTTGATGAAAGAAACAGCATTTAGCCATTTAATGGAGGTTGTTTTGGAAGTAGCTCCTGAAGTCTTGTACAATGGAATCTTCTCAAAGATCTTCAGGAACTCTTTATTTGACCTTTCGTCCCATCATTGTGGGAATTTTGTCATCCAAGCACTTATTTCTCATACAAGGGATCAGGATCAAATGGAGATAATCTGGGAGGAACTTGGTTCGAATTTCAAGGATCTGCTTAAAATGGGAAGATCTGGTGTTATTGCTTCGCTTATCACTGCAAGTCAAAGGCTTCATAGTTACGAACACAAGTGTTGTCAGGCTCTTGCTGCTGCAGTATATTCAAGCAGTGAGTCTTCAACATGCATTGTTCCCCGTATACTGTTTCTTGATAGCTACTTCAATTGTGAAGATAAGTCTAATTGGAGTTGGCCAAGTGGTGTTAAAATGCACGTCATGGGTTCTTTGATACTGCAAGAAGTTTTTCGATTTCAAATTGAATTTATACAGCCTTTTATTACAAGTATTACATCCATCACAGCTGATAATGTCCTTGAAGCTGCTAAGGATCCAGGGGTGCTCGTGATGCCTTTGTGTAAGCTTCAGTCCTATGGTTCTAAGGTAATTAAGACAAGATTTGATCAGATATTATGCTGTATACTTTCCAGGCTTCGTGGACATTTTGGAGAGCTAGCATTGCACTTGTCAGGTTCCTTTACTGTTGAAAAGTGCTTTACTACTGGTAACATTTCACAGAGGGAAGCCATAGTATCTGAGTTGGTAGCCGTGCAAAGTGAGCTTTGGAATACAAGGCAAGGTCCTCATCTTATGAGGAAACTAGATGTTGATGGATTTGCTGCTAGACCTGAGCAATGGAGGTCCAGACAAGAATCAAAGCAATCAACTTTGAGAGACTTTTCTGCTACATTTGGCTCTAGTGAGACCAGACCATCAAACAATAGTGAGACTGAGCAATCAAAGAAGAGCAGCTTTCTTTCTGATGATTATAAGAAAAAATCAAAAGTGGGAAAAACAGTTGACCATCACCAGGCATCTCCTGCACCATTTTTGAGAGTACAACAGGCTTCAAACGTCAATTATGGAAAACCTTCTAGTTGTGGAGACTATGAAACAGCTGGAATAGTAGTTTACTTGTTCTGCGTGGGATTCGAAGTACTAGGCGGGATCTTTCGGAAGCTTGAGCCAGCCAGCTTAAAAGGGCTCTTACTGTTTGCTTGCAAATCTTTGAGGGATTCTGAAGATGATGAAGCTCGGTTTGTTGGGGCAGATTTTAGCGGACGAGCCTGCGCTGTTGCTCCATTCGGCCAGGTTGATAATACCATAGAAAATGCAAGTACTAGAGCAGCAAGAATTCTAGACCAGGCCTTCATATCTGATCTCTTCTGTGGCTCTATGAATTCTGATTTTGGTGAAAATGATGATATATTTTTGCTGAGTAGGGGTCATGTGATTAGGCACGTGATTTTGGTATTAATAGTAATGGTGTTTTGGGAAGCTAAATGGGGAAGTATAATATTGCCATCAGTGATCAGGCAGTGGTACTGGGACTGGGGACAGTGTTGGATCTTGCAATATGATAATGTGGAGTTTGAAACAGGTGCTGTGGATATAAGTGAACCAGTTGGCTGA

Protein Analysis

1004

Amino Acids

112.55

Weight (kDa)

6.29

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF_NOP9 PF22493 373 - 725 3.3e-32 NOP9-like PUF repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2373
AccB1I GGYRCC 2 cut(s) 304, 739
AccB7I CCANNNNNTGG 1 cut(s) 1408
AccBSI CCGCTC 1 cut(s) 856
AccI GTMKAC 2 cut(s) 1744, 2023
AccII CGCG 2 cut(s) 974, 1114
AciI CCGC 5 cut(s) 712, 743, 856, 2547, 2661
AclWI GGATC 9 cut(s) 152, 922, 1554, 1560, 1605, 1928, 1941, 2558, 2957
AcoI YGGCCR 2 cut(s) 1796, 2689
AcsI RAATTY 6 cut(s) 204, 1510, 1588, 1865, 2736, 2782
AcuI CTGAAG 7 cut(s) 944, 1187, 1274, 1453, 1455, 1952, 2643
AcvI CACGTG 1 cut(s) 2845
AfeI AGCGCT 1 cut(s) 453
AfiI CCNNNNNNNGG 3 cut(s) 793, 1408, 2413
AflIII ACRYGT 2 cut(s) 972, 1138
AgeI ACCGGT 1 cut(s) 54
AhdI GACNNNNNGTC 2 cut(s) 598, 1492
AjiI CACGTC 1 cut(s) 1819
AjnI CCWGG 7 cut(s) 1147, 1316, 1936, 2030, 2412, 2691, 2745
Alw21I GWGCWC 2 cut(s) 1201, 1947
Alw26I GTCTC 5 cut(s) 389, 2271, 2300, 2324, 2481
AlwI GGATC 9 cut(s) 152, 922, 1554, 1560, 1605, 1928, 1941, 2558, 2957
AlwNI CAGNNNCTG 1 cut(s) 2987
Ama87I CYCGRG 3 cut(s) 9, 83, 548
Aor51HI AGCGCT 1 cut(s) 453
AoxI GGCC 4 cut(s) 13, 1796, 2689, 2748
ApeKI GCWGC 8 cut(s) 62, 313, 1694, 1697, 1925, 2222, 2352, 2729
ApoI RAATTY 6 cut(s) 204, 1510, 1588, 1865, 2736, 2782
AseI ATTAAT 1 cut(s) 2858
AsiGI ACCGGT 1 cut(s) 54
Asp700I GAANNNNTTC 4 cut(s) 341, 884, 1848, 2473
AspLEI GCGC 4 cut(s) 109, 454, 576, 2675
AspS9I GGNCC 4 cut(s) 405, 672, 2183, 2244
AsuC2I CCSGG 1 cut(s) 1157
AsuHPI GGTGA 3 cut(s) 1125, 2402, 2807
AsuII TTCGAA 2 cut(s) 1586, 2535
AsuNHI GCTAGC 2 cut(s) 116, 2056
AvaI CYCGRG 3 cut(s) 9, 83, 548
AvaII GGWCC 4 cut(s) 405, 672, 2183, 2244
BalI TGGCCA 1 cut(s) 1798
BamHI GGATCC 1 cut(s) 1933
BanI GGYRCC 2 cut(s) 304, 739
BanII GRGCYC 2 cut(s) 719, 2589
BauI CACGAG 1 cut(s) 1946
BbrPI CACGTG 1 cut(s) 2845
BbsI GAAGAC 2 cut(s) 1008, 1713
Bbv12I GWGCWC 2 cut(s) 1201, 1947
BbvI GCAGC 8 cut(s) 49, 325, 1681, 1684, 1912, 2209, 2364, 2741
BccI CCATC 9 cut(s) 407, 830, 944, 1506, 1907, 2207, 2323, 2415, 2914
BceAI ACGGC 3 cut(s) 810, 1024, 2135
BciT130I CCWGG 7 cut(s) 1149, 1318, 1938, 2032, 2414, 2693, 2747
BciVI GTATCC 1 cut(s) 524
BclI TGATCA 3 cut(s) 1228, 2005, 2913
BcnI CCSGG 1 cut(s) 1157
BcoDI GTCTC 5 cut(s) 389, 2271, 2300, 2324, 2481
BfmI CTRYAG 2 cut(s) 388, 1698
BfoI RGCGCY 1 cut(s) 455
BfuI GTATCC 1 cut(s) 524
BglI GCCNNNNNGGC 1 cut(s) 941
BglII AGATCT 2 cut(s) 1464, 1616
BisI GCNGC 8 cut(s) 63, 314, 1695, 1698, 1926, 2223, 2353, 2730
BlsI GCNGC 8 cut(s) 64, 315, 1696, 1699, 1927, 2224, 2354, 2731
BmcAI AGTACT 3 cut(s) 803, 2541, 2722
Bme1390I CCNGG 8 cut(s) 1149, 1157, 1318, 1938, 2032, 2414, 2693, 2747
Bme18I GGWCC 4 cut(s) 405, 672, 2183, 2244
BmeRI GACNNNNNGTC 2 cut(s) 598, 1492
BmeT110I CYCGRG 3 cut(s) 9, 83, 548
BmgBI CACGTC 1 cut(s) 1819
BmgT120I GGNCC 4 cut(s) 405, 672, 2183, 2244
BmiI GGNNCC 6 cut(s) 306, 361, 406, 741, 1935, 2077
BmrFI CCNGG 8 cut(s) 1149, 1157, 1318, 1938, 2032, 2414, 2693, 2747
BmrI ACTGGG 2 cut(s) 2938, 2944
BmtI GCTAGC 2 cut(s) 120, 2060
BmuI ACTGGG 2 cut(s) 2938, 2944
BpiI GAAGAC 2 cut(s) 1008, 1713
Bpu10I CCTNAGC 2 cut(s) 1929, 2232
Bpu14I TTCGAA 2 cut(s) 1586, 2535
BpuEI CTTGAG 1 cut(s) 2585
BpuMI CCSGG 1 cut(s) 1157
BsaAI YACGTR 1 cut(s) 2845
BsaBI GATNNNNATC 1 cut(s) 1551
BsaI GGTCTC 1 cut(s) 2300
BsaJI CCNNGG 3 cut(s) 10, 1317, 1937
BsaWI WCCGGW 1 cut(s) 54
Bsc4I CCNNNNNNNGG 3 cut(s) 793, 1408, 2413
Bse118I RCCGGY 1 cut(s) 54
Bse1I ACTGG 4 cut(s) 2110, 2933, 2939, 3005
Bse3DI GCAATG 6 cut(s) 264, 557, 576, 906, 2060, 2243
Bse8I GATNNNNATC 1 cut(s) 1551
BseBI CCWGG 7 cut(s) 1149, 1318, 1938, 2032, 2414, 2693, 2747
BseDI CCNNGG 3 cut(s) 10, 1317, 1937
BseGI GGATG 8 cut(s) 109, 151, 166, 1191, 1276, 1316, 1518, 1895
BseJI GATNNNNATC 1 cut(s) 1551
BseLI CCNNNNNNNGG 3 cut(s) 793, 1408, 2413
BseMI GCAATG 6 cut(s) 264, 557, 576, 906, 2060, 2243
BseMII CTCAG 5 cut(s) 1044, 2130, 2223, 2325, 2810
BseNI ACTGG 4 cut(s) 2110, 2933, 2939, 3005
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 8 cut(s) 49, 325, 1681, 1684, 1912, 2209, 2364, 2741
BsgI GTGCAG 1 cut(s) 2409
Bsh1236I CGCG 2 cut(s) 974, 1114
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 4 cut(s) 15, 1798, 2691, 2750
BshNI GGYRCC 2 cut(s) 304, 739
BshTI ACCGGT 1 cut(s) 54
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 2 cut(s) 1201, 1947
BsiHKCI CYCGRG 3 cut(s) 9, 83, 548
BsiSI CCGG 2 cut(s) 55, 1157
BslFI GGGAC 5 cut(s) 387, 418, 1480, 2945, 2952
BslI CCNNNNNNNGG 3 cut(s) 793, 1408, 2413
BsmAI GTCTC 5 cut(s) 389, 2271, 2300, 2324, 2481
BsmFI GGGAC 5 cut(s) 387, 418, 1480, 2945, 2952
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 4 cut(s) 15, 1798, 2691, 2750
Bso31I GGTCTC 1 cut(s) 2300
BsoBI CYCGRG 3 cut(s) 9, 83, 548
Bsp119I TTCGAA 2 cut(s) 1586, 2535
Bsp1286I GDGCHC 4 cut(s) 719, 1201, 1947, 2589
Bsp1407I TGTACA 1 cut(s) 1443
BspACI CCGC 5 cut(s) 712, 743, 856, 2547, 2661
BspANI GGCC 4 cut(s) 15, 1798, 2691, 2750
BspCNI CTCAG 5 cut(s) 1045, 2131, 2224, 2326, 2811
BspFNI CGCG 2 cut(s) 974, 1114
BspLI GGNNCC 6 cut(s) 306, 361, 406, 741, 1935, 2077
BspMAI CTGCAG 1 cut(s) 1702
BspOI GCTAGC 2 cut(s) 120, 2060
BspPI GGATC 9 cut(s) 152, 922, 1554, 1560, 1605, 1928, 1941, 2558, 2957
BspQI GCTCTTC 2 cut(s) 1272, 2342
BspT104I TTCGAA 2 cut(s) 1586, 2535
BspT107I GGYRCC 2 cut(s) 304, 739
BspTNI GGTCTC 1 cut(s) 2300
BsrBI CCGCTC 1 cut(s) 856
BsrDI GCAATG 6 cut(s) 264, 557, 576, 906, 2060, 2243
BsrFI RCCGGY 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 1443
BsrI ACTGG 4 cut(s) 2110, 2933, 2939, 3005
BssAI RCCGGY 1 cut(s) 54
BssECI CCNNGG 3 cut(s) 10, 1317, 1937
BssNAI GTATAC 2 cut(s) 1745, 2024
BssSI CACGAG 1 cut(s) 1946
Bst1107I GTATAC 2 cut(s) 1745, 2024
Bst2BI CACGAG 1 cut(s) 1946
Bst2UI CCWGG 7 cut(s) 1149, 1318, 1938, 2032, 2414, 2693, 2747
Bst6I CTCTTC 5 cut(s) 180, 1272, 1329, 2342, 2771
BstAPI GCANNNNNTGC 1 cut(s) 2423
BstAUI TGTACA 1 cut(s) 1443
BstBAI YACGTR 1 cut(s) 2845
BstBI TTCGAA 2 cut(s) 1586, 2535
BstC8I GCNNGC 7 cut(s) 118, 198, 2058, 2571, 2575, 2602, 2671
BstDEI CTNAG 9 cut(s) 241, 951, 1053, 1929, 1983, 2139, 2232, 2334, 2819
BstF5I GGATG 8 cut(s) 109, 151, 166, 1191, 1276, 1316, 1518, 1895
BstFNI CGCG 2 cut(s) 974, 1114
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 4 cut(s) 109, 454, 576, 2675
BstMAI GTCTC 5 cut(s) 389, 2271, 2300, 2324, 2481
BstMCI CGRYCG 1 cut(s) 136
BstNI CCWGG 7 cut(s) 1149, 1318, 1938, 2032, 2414, 2693, 2747
BstNSI RCATGY 2 cut(s) 1142, 1731
BstSCI CCNGG 8 cut(s) 1147, 1155, 1316, 1936, 2030, 2412, 2691, 2745
BstSFI CTRYAG 2 cut(s) 388, 1698
BstUI CGCG 2 cut(s) 974, 1114
BstV1I GCAGC 8 cut(s) 49, 325, 1681, 1684, 1912, 2209, 2364, 2741
BstV2I GAAGAC 2 cut(s) 1008, 1713
BstX2I RGATCY 8 cut(s) 157, 914, 1464, 1597, 1616, 1933, 2550, 2949
BstXI CCANNNNNNTGG 2 cut(s) 370, 1505
BstYI RGATCY 8 cut(s) 157, 914, 1464, 1597, 1616, 1933, 2550, 2949
BstZ17I GTATAC 2 cut(s) 1745, 2024
BsuI GTATCC 1 cut(s) 524
BsuRI GGCC 4 cut(s) 15, 1798, 2691, 2750
BtgZI GCGATG 1 cut(s) 1095
BtrI CACGTC 1 cut(s) 1819
BtsCI GGATG 8 cut(s) 109, 151, 166, 1191, 1276, 1316, 1518, 1895
BtsI GCAGTG 3 cut(s) 1641, 1720, 2927
BtsIMutI CAGTG 5 cut(s) 1641, 1720, 2916, 2927, 2948
Cac8I GCNNGC 7 cut(s) 118, 198, 2058, 2571, 2575, 2602, 2671
CaiI CAGNNNCTG 1 cut(s) 2987
CfoI GCGC 4 cut(s) 109, 454, 576, 2675
Cfr10I RCCGGY 1 cut(s) 54
Cfr13I GGNCC 4 cut(s) 405, 672, 2183, 2244
CseI GACGC 3 cut(s) 236, 305, 980
CspAI ACCGGT 1 cut(s) 54
CviAII CATG 6 cut(s) 97, 920, 1139, 1728, 1822, 2831
DdeI CTNAG 9 cut(s) 241, 951, 1053, 1929, 1983, 2139, 2232, 2334, 2819
DraI TTTAAA 1 cut(s) 1030
DriI GACNNNNNGTC 2 cut(s) 598, 1492
EaeI YGGCCR 2 cut(s) 1796, 2689
Eam1104I CTCTTC 5 cut(s) 180, 1272, 1329, 2342, 2771
Eam1105I GACNNNNNGTC 2 cut(s) 598, 1492
EarI CTCTTC 5 cut(s) 180, 1272, 1329, 2342, 2771
Eco147I AGGCCT 2 cut(s) 15, 2750
Eco24I GRGCYC 2 cut(s) 719, 2589
Eco31I GGTCTC 1 cut(s) 2300
Eco32I GATATC 1 cut(s) 499
Eco47I GGWCC 4 cut(s) 405, 672, 2183, 2244
Eco47III AGCGCT 1 cut(s) 453
Eco57I CTGAAG 7 cut(s) 944, 1187, 1274, 1453, 1455, 1952, 2643
Eco72I CACGTG 1 cut(s) 2845
Eco88I CYCGRG 3 cut(s) 9, 83, 548
EcoO109I RGGNCCY 1 cut(s) 2183
EcoRI GAATTC 2 cut(s) 2736, 2782
EcoRII CCWGG 7 cut(s) 1147, 1316, 1936, 2030, 2412, 2691, 2745
EcoRV GATATC 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 1733
EcoT38I GRGCYC 2 cut(s) 719, 2589
FaeI CATG 6 cut(s) 100, 923, 1142, 1731, 1825, 2834
FalI AAGNNNNNCTT 6 cut(s) 326, 358, 441, 473, 2339, 2371
FaqI GGGAC 5 cut(s) 387, 418, 1480, 2945, 2952
FatI CATG 6 cut(s) 96, 919, 1138, 1727, 1821, 2830
FauI CCCGC 3 cut(s) 705, 849, 2540
FbaI TGATCA 3 cut(s) 1228, 2005, 2913
FblI GTMKAC 2 cut(s) 1744, 2023
Fnu4HI GCNGC 8 cut(s) 63, 314, 1695, 1698, 1926, 2223, 2353, 2730
FokI GGATG 8 cut(s) 96, 153, 158, 1198, 1283, 1323, 1505, 1882
FriOI GRGCYC 2 cut(s) 719, 2589
Fsp4HI GCNGC 8 cut(s) 63, 314, 1695, 1698, 1926, 2223, 2353, 2730
GlaI GCGC 4 cut(s) 108, 453, 575, 2674
GluI GCNGC 8 cut(s) 63, 314, 1695, 1698, 1926, 2223, 2353, 2730
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 4 cut(s) 15, 1798, 2691, 2750
HapII CCGG 2 cut(s) 55, 1157
HgaI GACGC 3 cut(s) 236, 305, 980
HhaI GCGC 4 cut(s) 109, 454, 576, 2675
Hin1II CATG 6 cut(s) 100, 923, 1142, 1731, 1825, 2834
Hin6I GCGC 4 cut(s) 107, 452, 574, 2673
HinP1I GCGC 4 cut(s) 107, 452, 574, 2673
HincII GTYRAC 3 cut(s) 320, 784, 2404
HindII GTYRAC 3 cut(s) 320, 784, 2404
HindIII AAGCTT 2 cut(s) 1964, 2561
HinfI GANTC 9 cut(s) 721, 929, 1046, 1152, 1452, 1718, 2255, 2532, 2618
HpaII CCGG 2 cut(s) 55, 1157
HphI GGTGA 3 cut(s) 1125, 2402, 2807
Hpy166II GTNNAC 8 cut(s) 320, 784, 1745, 2024, 2042, 2404, 2515, 3002
Hpy8I GTNNAC 8 cut(s) 320, 784, 1745, 2024, 2042, 2404, 2515, 3002
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 2 cut(s) 2484, 2761
HpyCH4IV ACGT 4 cut(s) 610, 1818, 2457, 2844
HpyF3I CTNAG 9 cut(s) 241, 951, 1053, 1929, 1983, 2139, 2232, 2334, 2819
HpySE526I ACGT 4 cut(s) 610, 1818, 2457, 2844
Hsp92II CATG 6 cut(s) 100, 923, 1142, 1731, 1825, 2834
HspAI GCGC 4 cut(s) 107, 452, 574, 2673
Ksp22I TGATCA 3 cut(s) 1228, 2005, 2913
LguI GCTCTTC 2 cut(s) 1272, 2342
LmnI GCTCC 3 cut(s) 853, 1435, 2686
Lsp1109I GCAGC 8 cut(s) 49, 325, 1681, 1684, 1912, 2209, 2364, 2741
MaeII ACGT 4 cut(s) 610, 1818, 2457, 2844
MaeIII GTNAC 3 cut(s) 968, 1664, 2108
MbiI CCGCTC 1 cut(s) 856
MfeI CAATTG 1 cut(s) 1768
MflI RGATCY 8 cut(s) 157, 914, 1464, 1597, 1616, 1933, 2550, 2949
MhlI GDGCHC 4 cut(s) 719, 1201, 1947, 2589
MlsI TGGCCA 1 cut(s) 1798
MluI ACGCGT 1 cut(s) 972
MluNI TGGCCA 1 cut(s) 1798
MlyI GAGTC 2 cut(s) 938, 1727
MmeI TCCRAC 2 cut(s) 1023, 2927
Mox20I TGGCCA 1 cut(s) 1798
Mph1103I ATGCAT 1 cut(s) 1733
MroXI GAANNNNTTC 4 cut(s) 341, 884, 1848, 2473
MscI TGGCCA 1 cut(s) 1798
MslI CAYNNNNRTG 1 cut(s) 1503
Msp20I TGGCCA 1 cut(s) 1798
MspA1I CMGCKG 2 cut(s) 1907, 2501
MspI CCGG 2 cut(s) 55, 1157
MspR9I CCNGG 8 cut(s) 1149, 1157, 1318, 1938, 2032, 2414, 2693, 2747
MunI CAATTG 1 cut(s) 1768
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 7 cut(s) 1149, 1318, 1938, 2032, 2414, 2693, 2747
MvnI CGCG 2 cut(s) 974, 1114
NciI CCSGG 1 cut(s) 1157
NheI GCTAGC 2 cut(s) 116, 2056
NlaIII CATG 6 cut(s) 100, 923, 1142, 1731, 1825, 2834
NlaIV GGNNCC 6 cut(s) 306, 361, 406, 741, 1935, 2077
NmuCI GTSAC 1 cut(s) 968
NsiI ATGCAT 1 cut(s) 1733
NspI RCATGY 2 cut(s) 1142, 1731
NspV TTCGAA 2 cut(s) 1586, 2535
PaeR7I CTCGAG 1 cut(s) 548
PceI AGGCCT 2 cut(s) 15, 2750
PciI ACATGT 1 cut(s) 1138
PciSI GCTCTTC 2 cut(s) 1272, 2342
PcsI WCGNNNNNNNCGW 1 cut(s) 546
PctI GAATGC 1 cut(s) 269
PdmI GAANNNNTTC 4 cut(s) 341, 884, 1848, 2473
PfeI GAWTC 7 cut(s) 721, 1046, 1152, 1452, 2255, 2532, 2618
PflMI CCANNNNNTGG 1 cut(s) 1408
PfoI TCCNGGA 1 cut(s) 1155
PinAI ACCGGT 1 cut(s) 54
PkrI GCNGC 8 cut(s) 64, 315, 1696, 1699, 1927, 2224, 2354, 2731
PleI GAGTC 2 cut(s) 937, 1726
PmaCI CACGTG 1 cut(s) 2845
PmlI CACGTG 1 cut(s) 2845
PpsI GAGTC 2 cut(s) 937, 1726
Ppu21I YACGTR 1 cut(s) 2845
PpuMI RGGWCCY 1 cut(s) 2183
PscI ACATGT 1 cut(s) 1138
PshBI ATTAAT 1 cut(s) 2858
PsiI TTATAA 1 cut(s) 2373
Psp5II RGGWCCY 1 cut(s) 2183
Psp6I CCWGG 7 cut(s) 1147, 1316, 1936, 2030, 2412, 2691, 2745
PspCI CACGTG 1 cut(s) 2845
PspGI CCWGG 7 cut(s) 1147, 1316, 1936, 2030, 2412, 2691, 2745
PspN4I GGNNCC 6 cut(s) 306, 361, 406, 741, 1935, 2077
PspPI GGNCC 4 cut(s) 405, 672, 2183, 2244
PspPPI RGGWCCY 1 cut(s) 2183
PstI CTGCAG 1 cut(s) 1702
PstNI CAGNNNCTG 1 cut(s) 2987
PsuI RGATCY 8 cut(s) 157, 914, 1464, 1597, 1616, 1933, 2550, 2949
PvuII CAGCTG 2 cut(s) 1907, 2501
RseI CAYNNNNRTG 1 cut(s) 1503
SapI GCTCTTC 2 cut(s) 1272, 2342
SatI GCNGC 8 cut(s) 63, 314, 1695, 1698, 1926, 2223, 2353, 2730
Sau96I GGNCC 4 cut(s) 405, 672, 2183, 2244
ScaI AGTACT 3 cut(s) 803, 2541, 2722
SchI GAGTC 2 cut(s) 938, 1727
ScrFI CCNGG 8 cut(s) 1149, 1157, 1318, 1938, 2032, 2414, 2693, 2747
SduI GDGCHC 4 cut(s) 719, 1201, 1947, 2589
SfcI CTRYAG 2 cut(s) 388, 1698
Sfr274I CTCGAG 1 cut(s) 548
SfuI TTCGAA 2 cut(s) 1586, 2535
SinI GGWCC 4 cut(s) 405, 672, 2183, 2244
SlaI CTCGAG 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 1503
SmlI CTYRAG 2 cut(s) 548, 2564
SmoI CTYRAG 2 cut(s) 548, 2564
SseBI AGGCCT 2 cut(s) 15, 2750
SsiI CCGC 5 cut(s) 712, 743, 856, 2547, 2661
SspI AATATT 1 cut(s) 2901
StuI AGGCCT 2 cut(s) 15, 2750
StyD4I CCNGG 8 cut(s) 1147, 1155, 1316, 1936, 2030, 2412, 2691, 2745
TaiI ACGT 4 cut(s) 613, 1821, 2460, 2847
TaqI TCGA 9 cut(s) 136, 540, 549, 719, 828, 1201, 1586, 1852, 2535
TaqII GACCGA 2 cut(s) 122, 660
TatI WGTACW 9 cut(s) 237, 251, 801, 870, 1233, 1443, 2440, 2539, 2720
TfiI GAWTC 7 cut(s) 721, 1046, 1152, 1452, 2255, 2532, 2618
TscAI CASTG 5 cut(s) 1648, 1720, 2916, 2927, 2948
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 8 cut(s) 62, 313, 1694, 1697, 1925, 2222, 2352, 2729
Tsp45I GTSAC 1 cut(s) 968
TspRI CASTG 5 cut(s) 1648, 1720, 2916, 2927, 2948
Van91I CCANNNNNTGG 1 cut(s) 1408
VpaK11BI GGWCC 4 cut(s) 405, 672, 2183, 2244
VspI ATTAAT 1 cut(s) 2858
XapI RAATTY 6 cut(s) 204, 1510, 1588, 1865, 2736, 2782
XbaI TCTAGA 2 cut(s) 635, 2740
XceI RCATGY 2 cut(s) 1142, 1731
XhoI CTCGAG 1 cut(s) 548
XmiI GTMKAC 2 cut(s) 1744, 2023
XmnI GAANNNNTTC 4 cut(s) 341, 884, 1848, 2473
ZrmI AGTACT 3 cut(s) 803, 2541, 2722
Zsp2I ATGCAT 1 cut(s) 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.