Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0003362

Physical Location & Seq

Genomic Coordinates Forward (+)

66764240 .. 66769601

Gene Structure 5362 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG473100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2220 bp

ATGGTTGCTATTGGGTCTGCAGCATTGCCATTGCGACGACTCGAAAGCTCAATGGAGGACGACGGGTCGTATAAACGGGGTAGGAAGAAAATGGGTATGTCTAGAAAGCCTGAAAAACAAGGTCAAGGTCAAGGTCGGTCCTTTGATGCTGGTGTTAGTAAAACTGCATCCGGGCGGGGCTCGAATCGGGAGAGCTATTCGGCACCGCAGACTGTTGTGAGGAAACAGGTTGACCCCGAAACGGCAAAGTACTTTTCGGAGATTGCTAACCTTTTCGAGAGTGATGGGATTGATATTGAGGAGCGGTCGTCTTTATGTAGTAATGCGCTTGAAGAAACTAGGGGGAAAGAGCTGGAGCTTGCGACGGATTATATTATTAGTCACACTTTGCAGACTCTAGTTGAGGGCTGTGAAGTTGATTGCCTTTGTGCTTTTCTCCGAAGCTGTGCGAAGAACTTCCCTTTGATTGCAATGGATAAGTCTGGATCACATGTAGTTGAGTCTGCCATCAGGGCTTTATCTAAGCATCTTCAGGAGAGTGATGCGTATGCTGTTATTGAGGACACTCTAAATACAATATGCAAGGTAATTGTAAAGAATCCTGTTGATCTGATGTGTGATTGCTACGGATCTCATGTTCTACGAAGTCTTCTCTCTCTTTTTAAAGGAGTGCCGTTGGATTCTTCTGAGTTCCGAATGAGGAAGACATCTTCTGCTCTACCAGAACGGTTTAATTTCAAGGCATCGCGTTCAGACACGGATGTTTCACCACATTTTATACCTGGATTCCCGGATTTACTGAAGTTTCTTGTTTCCGGGATGTTAAAATGTGCTCGAAACAACATCAAAACCCTGCAAGTTGATCAGTACAGCAGTTTGGTTCTACAGACTACTCTGAAGTTGTTAGCAGGGGATGAAGAAGAGCTGTTGCAAATAATTCCCATTCTTATTGGATGTACCAGGGAAGATATTGAAGAAGAGAACTCCATTGAACCAACTATTGTGAGAGATGTTTTAGGTTTGATGAAAGAAACAGCATTTAGCCATTTAATGGAGCTTGTTTTGGAAGTAGCTCCTGAAGTCTTGTACAATGGAATCTTCTCAAAGATCTTCAGGAACTCTTTATTTGACCTTTCGTCCCATCATTGTGGGAATTTTGTCATCCAAGCACTTATTTCTCATACAAGGGATCAGGATCAAATGGAGATAATCTGGGAGGAACTTGGTTCGAATTTCAAGGATCTGCTTAAAATGGGAAGATCTGGTGTTATTGCTTCTCTTATCGCTGCAAGTCAAAGGCTTCATAGTTACGAACACAAGTGTTGTCAGGCTCTTGCTGCTGCAGTATATTCAAGCAGTGAGTCTTCAACATGCATTGTTCCCCGTATACTGTTTCTTGATAGCTACTTCAATTGTGAAGATAAGTCTAATTGGAGTTGGCCAAGCGGAGTTAAAATGCACGTCATGGGTTCTTTGATACTACAAGAAGTTTTTCGATTTCAAATTGAATTTATACAGCCTTTTATTACAAGTATTACATCCATCGCAGCTGATAATGTCCTTGAAGCTGCTAAGGATCCAGGGGGTGCTCGTGTTATTGAAGCATTTTTAAGTTCTAATGCTTCTGCAAAACTGAAACGCCGATTAGTTATGAGGCTTCGTGGACATTTTGGAGAGCTAGCATTGCACTCGTCAGGTTCCTTTACTGTTGAAAAGTGCTTTACTACTGGTAACATTTCACAGAGGGAAGCCATAGTATCTGAGTTGGTAGCCGTGCAAAGTGAGCTTTGGAATACAAGGCAAGGTCCTCATCTTATGAGGAAACTAGATGTTGATGGATTTGCTGCTAGACCTGAGCAGTGGAGGTCCAGACAAGAATCAAAGCAATCAACTTTGAGAGACTTTTCTGCTACATTTGGCTCTAGTGAGACCAAACCATCAAACAATAGTGAGACTGAGCAATCAAAGAAGAGCAGCTTTCTTTCTGATGATTATAAGAAAAAATCAAAAGTGGGAAAAACAGTTGACCATCACCAGGCATCTCCTGCACCATTTTTGAGTACAACAGGCTTCAAACGTAAGCCCAAGGAAAAAGAACAGAGCAACAAGAAGTTTGCAAGAGTCTCGGTAGATGAGGATGTTTCTAAAGTGAAGAATAAGTCAAAGAAGAAAAGGCATGGTGACGATGCTTTTTCTAAATCCCCCAAGTTGAAAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0008150 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0010033 GO:0010252 GO:0031974 GO:0031981 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0065007 GO:0065008 GO:0070013 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUF

Protein Analysis

739

Amino Acids

82.34

Weight (kDa)

8.31

Isoelectric Point (pI)

50.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF_NOP9	PF22493	115 - 599	4.2e-66	NOP9-like PUF repeat domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003362)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72320 AT1G72320 AT1G72320 AT1G72320
fragaria_vesca	FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860 FvH4_6g35860
malus_domestica	MD09G1172500.v1.1
prunus_persica	Prupe.3G030900_v2.0.a1 Prupe.3G030900_v2.0.a1 Prupe.3G030900_v2.0.a1 Prupe.3G030900_v2.0.a1 Prupe.3G030900_v2.0.a1 Prupe.3G030900_v2.0.a1
pyrus_communis	pycom09g09000
rosa_chinensis	RchiOBHm_Chr2g0147901
rosa_laevigata	RLG00000020291
rosa_multiflora	Rmu_sc0000308.1_g000059
rosa_roxburghii	Rroxscaffold_2G00099210
rosa_rugosa	Rorug02G0402900
rosa_samantha	Rh2AG459800 Rh2BG473100 Rh2CG447100 Rh2DG482000
rosa_wichuraiana	Rw2G037610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1995
AccB1I	GGYRCC	1 cut(s)	202
AccB7I	CCANNNNNTGG	1 cut(s)	1051
AccBSI	CCGCTC	1 cut(s)	304
AccI	GTMKAC	1 cut(s)	1387
AccII	CGCG	1 cut(s)	748
AciI	CCGC	4 cut(s)	175, 206, 304, 1446
AclWI	GGATC	7 cut(s)	493, 637, 1197, 1203, 1248, 1571, 1584
AcoI	YGGCCR	1 cut(s)	1439
AcsI	RAATTY	3 cut(s)	1153, 1231, 1508
AcuI	CTGAAG	5 cut(s)	515, 821, 917, 1096, 1098
AfaI	GTAC	5 cut(s)	251, 869, 958, 1088, 2062
AfiI	CCNNNNNNNGG	3 cut(s)	241, 1051, 2035
AflIII	ACRYGT	1 cut(s)	490
AhdI	GACNNNNNGTC	2 cut(s)	64, 1135
AjiI	CACGTC	1 cut(s)	1462
AjnI	CCWGG	4 cut(s)	781, 959, 1579, 2034
AloI	GAACNNNNNNTCC	2 cut(s)	621, 653
Alw21I	GWGCWC	2 cut(s)	835, 1591
Alw26I	GTCTC	4 cut(s)	1893, 1922, 1946, 2128
AlwI	GGATC	7 cut(s)	493, 637, 1197, 1203, 1248, 1571, 1584
AoxI	GGCC	1 cut(s)	1439
ApeKI	GCWGC	8 cut(s)	20, 1286, 1337, 1340, 1547, 1568, 1844, 1974
ApoI	RAATTY	3 cut(s)	1153, 1231, 1508
Asp700I	GAANNNNTTC	2 cut(s)	455, 1491
AspLEI	GCGC	1 cut(s)	328
AspS9I	GGNCC	3 cut(s)	138, 1805, 1866
AsuC2I	CCSGG	3 cut(s)	172, 791, 817
AsuHPI	GGTGA	3 cut(s)	759, 2024, 2192
AsuII	TTCGAA	1 cut(s)	1229
AsuNHI	GCTAGC	1 cut(s)	1678
AvaII	GGWCC	3 cut(s)	138, 1805, 1866
BalI	TGGCCA	1 cut(s)	1441
BamHI	GGATCC	1 cut(s)	1576
BanI	GGYRCC	1 cut(s)	202
BanII	GRGCYC	1 cut(s)	182
BauI	CACGAG	1 cut(s)	1590
BbsI	GAAGAC	3 cut(s)	641, 710, 1356
Bbv12I	GWGCWC	2 cut(s)	835, 1591
BbvI	GCAGC	8 cut(s)	32, 1273, 1324, 1327, 1555, 1559, 1831, 1986
BccI	CCATC	7 cut(s)	278, 515, 1149, 1550, 1829, 1945, 2037
BceAI	ACGGC	3 cut(s)	258, 658, 1757
BcgI	CGANNNNNNTGC	4 cut(s)	1671, 1705, 2106, 2140
BciT130I	CCWGG	4 cut(s)	783, 961, 1581, 2036
BclI	TGATCA	1 cut(s)	862
BcnI	CCSGG	3 cut(s)	172, 791, 817
BcoDI	GTCTC	4 cut(s)	1893, 1922, 1946, 2128
BfaI	CTAG	7 cut(s)	102, 339, 398, 1679, 1826, 1848, 1923
BfmI	CTRYAG	3 cut(s)	18, 884, 1341
BglI	GCCNNNNNGGC	1 cut(s)	512
BglII	AGATCT	2 cut(s)	1107, 1259
BisI	GCNGC	8 cut(s)	21, 1287, 1338, 1341, 1548, 1569, 1845, 1975
BlsI	GCNGC	8 cut(s)	22, 1288, 1339, 1342, 1549, 1570, 1846, 1976
BmcAI	AGTACT	1 cut(s)	251
Bme1390I	CCNGG	7 cut(s)	172, 783, 791, 817, 961, 1581, 2036
Bme18I	GGWCC	3 cut(s)	138, 1805, 1866
BmeRI	GACNNNNNGTC	2 cut(s)	64, 1135
BmgBI	CACGTC	1 cut(s)	1462
BmgT120I	GGNCC	3 cut(s)	138, 1805, 1866
BmiI	GGNNCC	3 cut(s)	204, 1578, 1699
BmrFI	CCNGG	7 cut(s)	172, 783, 791, 817, 961, 1581, 2036
BmsI	GCATC	7 cut(s)	136, 176, 532, 535, 752, 2048, 2176
BmtI	GCTAGC	1 cut(s)	1682
BpiI	GAAGAC	3 cut(s)	641, 710, 1356
BpmI	CTGGAG	1 cut(s)	374
Bpu10I	CCTNAGC	2 cut(s)	1572, 1854
Bpu14I	TTCGAA	1 cut(s)	1229
BpuMI	CCSGG	3 cut(s)	172, 791, 817
BsaBI	GATNNNNATC	1 cut(s)	1194
BsaI	GGTCTC	1 cut(s)	1922
BsaJI	CCNNGG	3 cut(s)	960, 1580, 2085
BsaXI	ACNNNNNCTCC	2 cut(s)	293, 323
Bsc4I	CCNNNNNNNGG	3 cut(s)	241, 1051, 2035
Bse1I	ACTGG	1 cut(s)	1732
Bse3DI	GCAATG	4 cut(s)	23, 29, 477, 1682
Bse8I	GATNNNNATC	1 cut(s)	1194
BseBI	CCWGG	4 cut(s)	783, 961, 1581, 2036
BseDI	CCNNGG	3 cut(s)	960, 1580, 2085
BseGI	GGATG	8 cut(s)	167, 766, 825, 919, 959, 1161, 1538, 2143
BseJI	GATNNNNATC	1 cut(s)	1194
BseLI	CCNNNNNNNGG	3 cut(s)	241, 1051, 2035
BseMI	GCAATG	4 cut(s)	23, 29, 477, 1682
BseMII	CTCAG	4 cut(s)	678, 1752, 1845, 1947
BseNI	ACTGG	1 cut(s)	1732
BseRI	GAGGAG	1 cut(s)	314
BseXI	GCAGC	8 cut(s)	32, 1273, 1324, 1327, 1555, 1559, 1831, 1986
BsgI	GTGCAG	1 cut(s)	2031
Bsh1236I	CGCG	1 cut(s)	748
Bsh1285I	CGRYCG	1 cut(s)	308
BshFI	GGCC	1 cut(s)	1441
BshNI	GGYRCC	1 cut(s)	202
BsiEI	CGRYCG	1 cut(s)	308
BsiHKAI	GWGCWC	2 cut(s)	835, 1591
BsiSI	CCGG	3 cut(s)	171, 791, 816
BslFI	GGGAC	1 cut(s)	1123
BslI	CCNNNNNNNGG	3 cut(s)	241, 1051, 2035
BsmAI	GTCTC	4 cut(s)	1893, 1922, 1946, 2128
BsmFI	GGGAC	1 cut(s)	1123
BsnI	GGCC	1 cut(s)	1441
Bso31I	GGTCTC	1 cut(s)	1922
Bsp119I	TTCGAA	1 cut(s)	1229
Bsp1286I	GDGCHC	3 cut(s)	182, 835, 1591
Bsp1407I	TGTACA	1 cut(s)	1086
BspACI	CCGC	4 cut(s)	175, 206, 304, 1446
BspANI	GGCC	1 cut(s)	1441
BspCNI	CTCAG	4 cut(s)	679, 1753, 1846, 1948
BspFNI	CGCG	1 cut(s)	748
BspLI	GGNNCC	3 cut(s)	204, 1578, 1699
BspMAI	CTGCAG	2 cut(s)	22, 1345
BspOI	GCTAGC	1 cut(s)	1682
BspPI	GGATC	7 cut(s)	493, 637, 1197, 1203, 1248, 1571, 1584
BspQI	GCTCTTC	2 cut(s)	915, 1964
BspT104I	TTCGAA	1 cut(s)	1229
BspT107I	GGYRCC	1 cut(s)	202
BspTNI	GGTCTC	1 cut(s)	1922
BsrBI	CCGCTC	1 cut(s)	304
BsrDI	GCAATG	4 cut(s)	23, 29, 477, 1682
BsrGI	TGTACA	1 cut(s)	1086
BsrI	ACTGG	1 cut(s)	1732
BssECI	CCNNGG	3 cut(s)	960, 1580, 2085
BssNAI	GTATAC	1 cut(s)	1388
BssSI	CACGAG	1 cut(s)	1590
BssT1I	CCWWGG	1 cut(s)	2085
Bst1107I	GTATAC	1 cut(s)	1388
Bst2BI	CACGAG	1 cut(s)	1590
Bst2UI	CCWGG	4 cut(s)	783, 961, 1581, 2036
Bst4CI	ACNGT	5 cut(s)	214, 729, 1392, 1708, 2023
Bst6I	CTCTTC	3 cut(s)	915, 972, 1964
BstAPI	GCANNNNNTGC	1 cut(s)	2045
BstAUI	TGTACA	1 cut(s)	1086
BstBI	TTCGAA	1 cut(s)	1229
BstC8I	GCNNGC	2 cut(s)	360, 1680
BstDEI	CTNAG	6 cut(s)	522, 687, 1572, 1761, 1854, 1956
BstF5I	GGATG	8 cut(s)	167, 766, 825, 919, 959, 1161, 1538, 2143
BstFNI	CGCG	1 cut(s)	748
BstHHI	GCGC	1 cut(s)	328
BstMAI	GTCTC	4 cut(s)	1893, 1922, 1946, 2128
BstMCI	CGRYCG	1 cut(s)	308
BstMWI	GCNNNNNNNGC	5 cut(s)	512, 1337, 1684, 1783, 2045
BstNI	CCWGG	4 cut(s)	783, 961, 1581, 2036
BstNSI	RCATGY	2 cut(s)	494, 1374
BstSCI	CCNGG	7 cut(s)	170, 781, 789, 815, 959, 1579, 2034
BstSFI	CTRYAG	3 cut(s)	18, 884, 1341
BstUI	CGCG	1 cut(s)	748
BstV1I	GCAGC	8 cut(s)	32, 1273, 1324, 1327, 1555, 1559, 1831, 1986
BstV2I	GAAGAC	3 cut(s)	641, 710, 1356
BstX2I	RGATCY	5 cut(s)	629, 1107, 1240, 1259, 1576
BstXI	CCANNNNNNTGG	1 cut(s)	1148
BstYI	RGATCY	5 cut(s)	629, 1107, 1240, 1259, 1576
BstZ17I	GTATAC	1 cut(s)	1388
BsuRI	GGCC	1 cut(s)	1441
BtgZI	GCGATG	2 cut(s)	729, 1528
BtrI	CACGTC	1 cut(s)	1462
BtsCI	GGATG	8 cut(s)	167, 766, 825, 919, 959, 1161, 1538, 2143
BtsI	GCAGTG	2 cut(s)	1363, 1865
BtsIMutI	CAGTG	2 cut(s)	1363, 1865
Cac8I	GCNNGC	2 cut(s)	360, 1680
CfoI	GCGC	1 cut(s)	328
Cfr13I	GGNCC	3 cut(s)	138, 1805, 1866
Csp6I	GTAC	5 cut(s)	250, 868, 957, 1087, 2061
CviAII	CATG	6 cut(s)	491, 635, 1371, 1465, 2177, 2217
CviQI	GTAC	5 cut(s)	250, 868, 957, 1087, 2061
DdeI	CTNAG	6 cut(s)	522, 687, 1572, 1761, 1854, 1956
DraI	TTTAAA	1 cut(s)	664
DriI	GACNNNNNGTC	2 cut(s)	64, 1135
EaeI	YGGCCR	1 cut(s)	1439
Eam1104I	CTCTTC	3 cut(s)	915, 972, 1964
Eam1105I	GACNNNNNGTC	2 cut(s)	64, 1135
EarI	CTCTTC	3 cut(s)	915, 972, 1964
Eco130I	CCWWGG	1 cut(s)	2085
Eco24I	GRGCYC	1 cut(s)	182
Eco31I	GGTCTC	1 cut(s)	1922
Eco47I	GGWCC	3 cut(s)	138, 1805, 1866
Eco57I	CTGAAG	5 cut(s)	515, 821, 917, 1096, 1098
EcoO109I	RGGNCCY	1 cut(s)	1805
EcoRII	CCWGG	4 cut(s)	781, 959, 1579, 2034
EcoT14I	CCWWGG	1 cut(s)	2085
EcoT22I	ATGCAT	1 cut(s)	1376
EcoT38I	GRGCYC	1 cut(s)	182
ErhI	CCWWGG	1 cut(s)	2085
FaeI	CATG	6 cut(s)	494, 638, 1374, 1468, 2180, 2220
FalI	AAGNNNNNCTT	2 cut(s)	1961, 1993
FaqI	GGGAC	1 cut(s)	1123
FatI	CATG	6 cut(s)	490, 634, 1370, 1464, 2176, 2216
FauI	CCCGC	1 cut(s)	168
FbaI	TGATCA	1 cut(s)	862
FblI	GTMKAC	1 cut(s)	1387
Fnu4HI	GCNGC	8 cut(s)	21, 1287, 1338, 1341, 1548, 1569, 1845, 1975
FokI	GGATG	8 cut(s)	154, 773, 832, 926, 966, 1148, 1525, 2150
FriOI	GRGCYC	1 cut(s)	182
Fsp4HI	GCNGC	8 cut(s)	21, 1287, 1338, 1341, 1548, 1569, 1845, 1975
FspBI	CTAG	7 cut(s)	102, 339, 398, 1679, 1826, 1848, 1923
GlaI	GCGC	1 cut(s)	327
GluI	GCNGC	8 cut(s)	21, 1287, 1338, 1341, 1548, 1569, 1845, 1975
GsuI	CTGGAG	1 cut(s)	374
HaeIII	GGCC	1 cut(s)	1441
HapII	CCGG	3 cut(s)	171, 791, 816
HhaI	GCGC	1 cut(s)	328
Hin1II	CATG	6 cut(s)	494, 638, 1374, 1468, 2180, 2220
Hin6I	GCGC	1 cut(s)	326
HinP1I	GCGC	1 cut(s)	326
HincII	GTYRAC	2 cut(s)	232, 2026
HindII	GTYRAC	2 cut(s)	232, 2026
HpaII	CCGG	3 cut(s)	171, 791, 816
HphI	GGTGA	3 cut(s)	759, 2024, 2192
Hpy166II	GTNNAC	4 cut(s)	232, 1388, 1664, 2026
Hpy188I	TCNGA	9 cut(s)	259, 440, 612, 688, 695, 754, 897, 1762, 1987
Hpy8I	GTNNAC	4 cut(s)	232, 1388, 1664, 2026
Hpy99I	CGWCG	3 cut(s)	39, 65, 367
HpyCH4III	ACNGT	5 cut(s)	214, 729, 1392, 1708, 2023
HpyCH4IV	ACGT	2 cut(s)	1461, 2077
HpyF10VI	GCNNNNNNNGC	5 cut(s)	512, 1337, 1684, 1783, 2045
HpyF3I	CTNAG	6 cut(s)	522, 687, 1572, 1761, 1854, 1956
HpySE526I	ACGT	2 cut(s)	1461, 2077
Hsp92II	CATG	6 cut(s)	494, 638, 1374, 1468, 2180, 2220
HspAI	GCGC	1 cut(s)	326
Ksp22I	TGATCA	1 cut(s)	862
LguI	GCTCTTC	2 cut(s)	915, 1964
LmnI	GCTCC	4 cut(s)	301, 355, 1054, 1078
Lsp1109I	GCAGC	8 cut(s)	32, 1273, 1324, 1327, 1555, 1559, 1831, 1986
LweI	GCATC	7 cut(s)	136, 176, 532, 535, 752, 2048, 2176
MaeI	CTAG	7 cut(s)	102, 339, 398, 1679, 1826, 1848, 1923
MaeII	ACGT	2 cut(s)	1461, 2077
MaeIII	GTNAC	4 cut(s)	380, 1307, 1730, 2180
MbiI	CCGCTC	1 cut(s)	304
MfeI	CAATTG	1 cut(s)	1411
MflI	RGATCY	5 cut(s)	629, 1107, 1240, 1259, 1576
MhlI	GDGCHC	3 cut(s)	182, 835, 1591
MlsI	TGGCCA	1 cut(s)	1441
MluCI	AATT	9 cut(s)	588, 733, 936, 1153, 1231, 1411, 1429, 1503, 1508
MluNI	TGGCCA	1 cut(s)	1441
MlyI	GAGTC	5 cut(s)	33, 388, 509, 1370, 2130
MmeI	TCCRAC	1 cut(s)	657
Mox20I	TGGCCA	1 cut(s)	1441
Mph1103I	ATGCAT	1 cut(s)	1376
MroXI	GAANNNNTTC	2 cut(s)	455, 1491
MscI	TGGCCA	1 cut(s)	1441
MseI	TTAA	7 cut(s)	663, 732, 824, 1049, 1248, 1452, 1610
MslI	CAYNNNNRTG	1 cut(s)	1146
Msp20I	TGGCCA	1 cut(s)	1441
MspA1I	CMGCKG	1 cut(s)	1550
MspI	CCGG	3 cut(s)	171, 791, 816
MspR9I	CCNGG	7 cut(s)	172, 783, 791, 817, 961, 1581, 2036
MunI	CAATTG	1 cut(s)	1411
MvaI	CCWGG	4 cut(s)	783, 961, 1581, 2036
MvnI	CGCG	1 cut(s)	748
MwoI	GCNNNNNNNGC	5 cut(s)	512, 1337, 1684, 1783, 2045
NciI	CCSGG	3 cut(s)	172, 791, 817
NheI	GCTAGC	1 cut(s)	1678
NlaIII	CATG	6 cut(s)	494, 638, 1374, 1468, 2180, 2220
NlaIV	GGNNCC	3 cut(s)	204, 1578, 1699
NmuCI	GTSAC	2 cut(s)	380, 2180
NsiI	ATGCAT	1 cut(s)	1376
NspI	RCATGY	2 cut(s)	494, 1374
NspV	TTCGAA	1 cut(s)	1229
PciI	ACATGT	1 cut(s)	490
PciSI	GCTCTTC	2 cut(s)	915, 1964
PdmI	GAANNNNTTC	2 cut(s)	455, 1491
PfeI	GAWTC	6 cut(s)	184, 598, 680, 786, 1095, 1877
PflMI	CCANNNNNTGG	1 cut(s)	1051
PfoI	TCCNGGA	2 cut(s)	789, 815
PkrI	GCNGC	8 cut(s)	22, 1288, 1339, 1342, 1549, 1570, 1846, 1976
PleI	GAGTC	5 cut(s)	33, 388, 508, 1369, 2129
PpsI	GAGTC	5 cut(s)	33, 388, 508, 1369, 2129
PpuMI	RGGWCCY	1 cut(s)	1805
PscI	ACATGT	1 cut(s)	490
PsiI	TTATAA	1 cut(s)	1995
Psp5II	RGGWCCY	1 cut(s)	1805
Psp6I	CCWGG	4 cut(s)	781, 959, 1579, 2034
PspGI	CCWGG	4 cut(s)	781, 959, 1579, 2034
PspN4I	GGNNCC	3 cut(s)	204, 1578, 1699
PspPI	GGNCC	3 cut(s)	138, 1805, 1866
PspPPI	RGGWCCY	1 cut(s)	1805
PstI	CTGCAG	2 cut(s)	22, 1345
PsuI	RGATCY	5 cut(s)	629, 1107, 1240, 1259, 1576
PvuII	CAGCTG	1 cut(s)	1550
RsaI	GTAC	5 cut(s)	251, 869, 958, 1088, 2062
RsaNI	GTAC	5 cut(s)	250, 868, 957, 1087, 2061
RseI	CAYNNNNRTG	1 cut(s)	1146
SapI	GCTCTTC	2 cut(s)	915, 1964
SaqAI	TTAA	7 cut(s)	663, 732, 824, 1049, 1248, 1452, 1610
SatI	GCNGC	8 cut(s)	21, 1287, 1338, 1341, 1548, 1569, 1845, 1975
Sau96I	GGNCC	3 cut(s)	138, 1805, 1866
ScaI	AGTACT	1 cut(s)	251
SchI	GAGTC	5 cut(s)	33, 388, 509, 1370, 2130
ScrFI	CCNGG	7 cut(s)	172, 783, 791, 817, 961, 1581, 2036
SduI	GDGCHC	3 cut(s)	182, 835, 1591
SfaNI	GCATC	7 cut(s)	136, 176, 532, 535, 752, 2048, 2176
SfcI	CTRYAG	3 cut(s)	18, 884, 1341
SfuI	TTCGAA	1 cut(s)	1229
SinI	GGWCC	3 cut(s)	138, 1805, 1866
SmiMI	CAYNNNNRTG	1 cut(s)	1146
Sse9I	AATT	9 cut(s)	588, 733, 936, 1153, 1231, 1411, 1429, 1503, 1508
SsiI	CCGC	4 cut(s)	175, 206, 304, 1446
SspMI	CTAG	7 cut(s)	102, 339, 398, 1679, 1826, 1848, 1923
StyD4I	CCNGG	7 cut(s)	170, 781, 789, 815, 959, 1579, 2034
StyI	CCWWGG	1 cut(s)	2085
TaaI	ACNGT	5 cut(s)	214, 729, 1392, 1708, 2023
TaiI	ACGT	2 cut(s)	1464, 2080
TaqI	TCGA	6 cut(s)	42, 182, 276, 835, 1229, 1495
TaqII	GACCGA	1 cut(s)	126
TasI	AATT	9 cut(s)	588, 733, 936, 1153, 1231, 1411, 1429, 1503, 1508
TatI	WGTACW	4 cut(s)	249, 867, 1086, 2060
TfiI	GAWTC	6 cut(s)	184, 598, 680, 786, 1095, 1877
Tru1I	TTAA	7 cut(s)	663, 732, 824, 1049, 1248, 1452, 1610
Tru9I	TTAA	7 cut(s)	663, 732, 824, 1049, 1248, 1452, 1610
TscAI	CASTG	2 cut(s)	1363, 1865
TseFI	GTSAC	2 cut(s)	380, 2180
TseI	GCWGC	8 cut(s)	20, 1286, 1337, 1340, 1547, 1568, 1844, 1974
Tsp45I	GTSAC	2 cut(s)	380, 2180
TspDTI	ATGAA	3 cut(s)	930, 1040, 1292
TspGWI	ACGGA	3 cut(s)	380, 642, 773
TspRI	CASTG	2 cut(s)	1363, 1865
Van91I	CCANNNNNTGG	1 cut(s)	1051
VpaK11BI	GGWCC	3 cut(s)	138, 1805, 1866
XapI	RAATTY	3 cut(s)	1153, 1231, 1508
XbaI	TCTAGA	1 cut(s)	101
XceI	RCATGY	2 cut(s)	494, 1374
XmiI	GTMKAC	1 cut(s)	1387
XmnI	GAANNNNTTC	2 cut(s)	455, 1491
XspI	CTAG	7 cut(s)	102, 339, 398, 1679, 1826, 1848, 1923
ZrmI	AGTACT	1 cut(s)	251
Zsp2I	ATGCAT	1 cut(s)	1376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.