FvH4_6g38730

Pleckstrin homology domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012240
Physical Location & Seq
Forward (+)
30637966 .. 30642910
4945 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38730.t1

Sequence Viewer

Length: 1497 bp
ATGGCCTCCAATGGTGCTTCTCCGAGAGTTGCAGACCCAGAGAATAGCTTGGAGAAGATCAAGCGCCAGCTCGCTTCCGGGTCGGGTCGGAACTTGCTCCAGGGCCCACTTCTCAAGCGATCCGAGACTCTGCGGAAATGGAACGAGCGGTGGGTGATCTTGGACCCAACAACAGGAAGAATGGAATACAAGATTAGGAGAAATGAGCCGAGTGTGAAGGGGACCATTCTGTTTGATGCAAACAGCACAATCACCCTGTCTCCTGTGAACTTCCATGGGATGCCAAAGTATGACGGCTGCTGTTTCTATATTGGGACCCCGCAGAAAAAAGATTATTTCCTTTGTGCAGAAACTCCCGGTGCAGCTAGAGCTTGGGTGTCAACGTTACAAGCAACACAGTTGGTACTAAAGGCCCATAAAGAAGCTGTGAATTCCTTGAGTGGCAATGGTTCAGCAAAACTAGGTACTGTTGCAGCAGTTGTTGCTGCTGCGAATTCAACTGCTGCAGAGAGTTCAAAGGAAATTGAAGCAGCAATGCATATCTCTTTGAGAAATGCTTTGGGAATGATAACCCAGAAGCCAACCGATGGTCCCATGGATGATTTTGCAGTAATGAAGGAGACACTGAAGGTTAAAGATGAGGAACTACAAAATTTGGCTCGGGACGTACGAGCACGTGATTCAACAATTAAAGAACTTGCAGAGAAATTAACTGAGACTGCTGAATCTGCTGAGGCTGCAGCCTCAGCAGCTCATACAATTGATGAACAAAGGAGAATTGCTTGTGCAGAAATCGAGCGTTTAAGAAGAGAATCAGAGAAGCAGCAGGAATCTTCCATGTCAAAGCTAAAAGATTCTGAAGCAAAGGTTATGGACTTGATCAAAGAAAGAGAGCAAGTGATTAGGCAGAGAGACTCTGCTCATCAGGAGGCTCACATGTGGCGTTCTGAACTTGCTAAAGCTAGAGAGCGTGTTGTGATATTAGAGGCAGCCGTTGTCAGAGCCGAAGAGAAGGTCAGGGTTGCAGATTCAGATGCTGAAGCTAGAATAAAGGAGGCTGTGCAGAAAGAGTCAGCTGCACTGAATGACAAGCAGGAGCTCCTTGTCTATGTAAATAAGTTACAAGCACAACTCCAGAGAGAGCATGTGGATAAGCAAGTGTTCGAGGAGAAGTCAGAGTCTTGCTCAGATATTGGCAATACACCCCTGACAAAACATATAGACTTATCAGAGGAAAATGTTGATAAAGCATGCCTTAGTGTTTCTAAATCAGTTCCAGTACCGGGAGAGAGTGTAGTTCACATGTCAGGGGATCAAGCCAACCTCCGGCCAGTTGGAGATGGTGAATGGAGTGATATTCAGGCCACAGAGTCGAGGATAGCCGATGTAAGAGAAATCGCCCCCGAATCAGAAACAAGCAGTTTAGATATTCCTGTTGTTAGCCAACCAGTTGGTAACCATCACGATCAAGGGGGAAACTCTTCTCTACAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

499

Amino Acids

54.78

Weight (kDa)

5.66

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 33 - 131 1.8e-06 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 587
AccBSI CCGCTC 1 cut(s) 148
AciI CCGC 3 cut(s) 133, 148, 320
AclI AACGTT 1 cut(s) 383
AclWI GGATC 2 cut(s) 114, 1320
AcoI YGGCCR 1 cut(s) 1328
AcsI RAATTY 3 cut(s) 430, 493, 652
AcuI CTGAAG 3 cut(s) 647, 879, 1059
AcvI CACGTG 1 cut(s) 677
AfaI GTAC 4 cut(s) 405, 466, 669, 1281
AfiI CCNNNNNNNGG 4 cut(s) 173, 587, 1283, 1326
AflIII ACRYGT 2 cut(s) 936, 1302
AgsI TTSAA 4 cut(s) 498, 516, 527, 684
AjnI CCWGG 1 cut(s) 99
Alw21I GWGCWC 2 cut(s) 676, 1101
Alw26I GTCTC 5 cut(s) 119, 264, 614, 710, 906
AlwI GGATC 2 cut(s) 114, 1320
AlwNI CAGNNNCTG 1 cut(s) 1037
Ama87I CYCGRG 1 cut(s) 660
AoxI GGCC 5 cut(s) 3, 103, 411, 1328, 1362
ApaI GGGCCC 1 cut(s) 107
ApoI RAATTY 3 cut(s) 430, 493, 652
ArsI GACNNNNNNTTYG 2 cut(s) 999, 1031
Asp700I GAANNNNTTC 1 cut(s) 1480
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 7 cut(s) 103, 104, 163, 222, 315, 412, 590
AsuC2I CCSGG 3 cut(s) 79, 357, 1284
AsuHPI GGTGA 3 cut(s) 166, 244, 1355
AvaI CYCGRG 1 cut(s) 660
AvaII GGWCC 4 cut(s) 163, 222, 315, 590
BaeGI GKGCMC 1 cut(s) 107
BanII GRGCYC 2 cut(s) 107, 1101
BbrPI CACGTG 1 cut(s) 677
Bbv12I GWGCWC 2 cut(s) 676, 1101
BbvCI CCTCAGC 2 cut(s) 732, 745
BccI CCATC 3 cut(s) 581, 1334, 1467
BceAI ACGGC 2 cut(s) 310, 977
BciT130I CCWGG 1 cut(s) 101
BclI TGATCA 1 cut(s) 879
BcnI CCSGG 3 cut(s) 79, 357, 1284
BcoDI GTCTC 5 cut(s) 119, 264, 614, 710, 906
BfaI CTAG 4 cut(s) 366, 461, 963, 1044
BfmI CTRYAG 3 cut(s) 504, 738, 1487
BfoI RGCGCY 1 cut(s) 67
Bme1390I CCNGG 4 cut(s) 79, 101, 357, 1284
Bme18I GGWCC 4 cut(s) 163, 222, 315, 590
BmeT110I CYCGRG 1 cut(s) 660
BmgT120I GGNCC 7 cut(s) 103, 104, 163, 222, 315, 412, 590
BmiI GGNNCC 6 cut(s) 105, 165, 223, 316, 317, 592
BmrFI CCNGG 4 cut(s) 79, 101, 357, 1284
BmsI GCATC 3 cut(s) 226, 270, 1024
BplI GAGNNNNNCTC 2 cut(s) 1169, 1201
BpmI CTGGAG 2 cut(s) 83, 1118
Bpu10I CCTNAGC 2 cut(s) 732, 745
BpuEI CTTGAG 2 cut(s) 98, 457
BpuMI CCSGG 3 cut(s) 79, 357, 1284
BsaAI YACGTR 1 cut(s) 677
BsaJI CCNNGG 3 cut(s) 100, 274, 594
BsaXI ACNNNNNCTCC 4 cut(s) 244, 274, 1278, 1308
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 587, 1283, 1326
Bse1I ACTGG 3 cut(s) 1277, 1331, 1448
Bse3DI GCAATG 2 cut(s) 451, 540
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 3 cut(s) 100, 274, 594
BseGI GGATG 2 cut(s) 285, 604
BseLI CCNNNNNNNGG 4 cut(s) 173, 587, 1283, 1326
BseMI GCAATG 2 cut(s) 451, 540
BseMII CTCAG 4 cut(s) 705, 723, 759, 1200
BseNI ACTGG 3 cut(s) 1277, 1331, 1448
BseRI GAGGAG 1 cut(s) 1181
BseSI GKGCMC 1 cut(s) 107
BsgI GTGCAG 5 cut(s) 366, 381, 807, 1062, 1082
BshFI GGCC 5 cut(s) 5, 105, 413, 1330, 1364
BsiHKAI GWGCWC 2 cut(s) 676, 1101
BsiHKCI CYCGRG 1 cut(s) 660
BsiSI CCGG 4 cut(s) 78, 357, 1283, 1327
BsiWI CGTACG 1 cut(s) 667
BslFI GGGAC 4 cut(s) 235, 328, 576, 677
BslI CCNNNNNNNGG 4 cut(s) 173, 587, 1283, 1326
BsmAI GTCTC 5 cut(s) 119, 264, 614, 710, 906
BsmFI GGGAC 4 cut(s) 235, 328, 576, 677
BsnI GGCC 5 cut(s) 5, 105, 413, 1330, 1364
BsoBI CYCGRG 1 cut(s) 660
Bsp120I GGGCCC 1 cut(s) 103
Bsp1286I GDGCHC 3 cut(s) 107, 676, 1101
Bsp143I GATC 6 cut(s) 57, 119, 156, 879, 1312, 1465
Bsp19I CCATGG 2 cut(s) 274, 594
BspACI CCGC 3 cut(s) 133, 148, 320
BspANI GGCC 5 cut(s) 5, 105, 413, 1330, 1364
BspCNI CTCAG 4 cut(s) 706, 724, 758, 1199
BspLI GGNNCC 6 cut(s) 105, 165, 223, 316, 317, 592
BspMAI CTGCAG 2 cut(s) 508, 742
BspPI GGATC 2 cut(s) 114, 1320
BsrBI CCGCTC 1 cut(s) 148
BsrDI GCAATG 2 cut(s) 451, 540
BsrI ACTGG 3 cut(s) 1277, 1331, 1448
BssECI CCNNGG 3 cut(s) 100, 274, 594
BssMI GATC 6 cut(s) 57, 119, 156, 879, 1312, 1465
BssT1I CCWWGG 2 cut(s) 274, 594
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 2 cut(s) 399, 469
Bst6I CTCTTC 3 cut(s) 802, 1002, 1486
BstAPI GCANNNNNTGC 1 cut(s) 482
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 3 cut(s) 68, 72, 1252
BstDEI CTNAG 5 cut(s) 714, 732, 745, 1186, 1256
BstDSI CCRYGG 2 cut(s) 274, 594
BstEII GGTNACC 1 cut(s) 1454
BstF5I GGATG 2 cut(s) 285, 604
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 6 cut(s) 60, 122, 159, 882, 1315, 1468
BstMAI GTCTC 5 cut(s) 119, 264, 614, 710, 906
BstMBI GATC 6 cut(s) 57, 119, 156, 879, 1312, 1465
BstMWI GCNNNNNNNGC 6 cut(s) 368, 482, 728, 737, 746, 749
BstNI CCWGG 1 cut(s) 101
BstNSI RCATGY 4 cut(s) 940, 1148, 1254, 1306
BstPI GGTNACC 1 cut(s) 1454
BstSCI CCNGG 4 cut(s) 77, 99, 355, 1282
BstSFI CTRYAG 3 cut(s) 504, 738, 1487
BstSLI GKGCMC 1 cut(s) 107
BstXI CCANNNNNNTGG 1 cut(s) 1451
BsuRI GGCC 5 cut(s) 5, 105, 413, 1330, 1364
BtgI CCRYGG 2 cut(s) 274, 594
BtsCI GGATG 2 cut(s) 285, 604
BtsIMutI CAGTG 2 cut(s) 623, 1079
Cac8I GCNNGC 3 cut(s) 68, 72, 1252
CaiI CAGNNNCTG 1 cut(s) 1037
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 7 cut(s) 103, 104, 163, 222, 315, 412, 590
Csp6I GTAC 4 cut(s) 404, 465, 668, 1280
CviAII CATG 7 cut(s) 275, 595, 838, 937, 1145, 1251, 1303
CviQI GTAC 4 cut(s) 404, 465, 668, 1280
DdeI CTNAG 5 cut(s) 714, 732, 745, 1186, 1256
DpnI GATC 6 cut(s) 59, 121, 158, 881, 1314, 1467
DpnII GATC 6 cut(s) 57, 119, 156, 879, 1312, 1465
EaeI YGGCCR 1 cut(s) 1328
Eam1104I CTCTTC 3 cut(s) 802, 1002, 1486
EarI CTCTTC 3 cut(s) 802, 1002, 1486
Ecl136II GAGCTC 1 cut(s) 1099
Eco130I CCWWGG 2 cut(s) 274, 594
Eco24I GRGCYC 2 cut(s) 107, 1101
Eco47I GGWCC 4 cut(s) 163, 222, 315, 590
Eco53kI GAGCTC 1 cut(s) 1099
Eco57I CTGAAG 3 cut(s) 647, 879, 1059
Eco72I CACGTG 1 cut(s) 677
Eco88I CYCGRG 1 cut(s) 660
Eco91I GGTNACC 1 cut(s) 1454
EcoICRI GAGCTC 1 cut(s) 1099
EcoO109I RGGNCCY 2 cut(s) 103, 315
EcoO65I GGTNACC 1 cut(s) 1454
EcoRI GAATTC 2 cut(s) 430, 493
EcoRII CCWGG 1 cut(s) 99
EcoT14I CCWWGG 2 cut(s) 274, 594
EcoT22I ATGCAT 1 cut(s) 540
EcoT38I GRGCYC 2 cut(s) 107, 1101
ErhI CCWWGG 2 cut(s) 274, 594
FaeI CATG 7 cut(s) 278, 598, 841, 940, 1148, 1254, 1306
FalI AAGNNNNNCTT 2 cut(s) 1239, 1271
FaqI GGGAC 4 cut(s) 235, 328, 576, 677
FatI CATG 7 cut(s) 274, 594, 837, 936, 1144, 1250, 1302
FauI CCCGC 1 cut(s) 327
FbaI TGATCA 1 cut(s) 879
FokI GGATG 2 cut(s) 292, 611
FriOI GRGCYC 2 cut(s) 107, 1101
FspBI CTAG 4 cut(s) 366, 461, 963, 1044
GlaI GCGC 1 cut(s) 65
GsuI CTGGAG 2 cut(s) 83, 1118
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 5 cut(s) 5, 105, 413, 1330, 1364
HapII CCGG 4 cut(s) 78, 357, 1283, 1327
HhaI GCGC 1 cut(s) 66
Hin1II CATG 7 cut(s) 278, 598, 841, 940, 1148, 1254, 1306
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HpaII CCGG 4 cut(s) 78, 357, 1283, 1327
HphI GGTGA 3 cut(s) 166, 244, 1355
Hpy166II GTNNAC 3 cut(s) 268, 381, 1300
Hpy188III TCNNGA 4 cut(s) 662, 926, 1135, 1463
Hpy8I GTNNAC 3 cut(s) 268, 381, 1300
HpyAV CCTTC 4 cut(s) 211, 610, 622, 1006
HpyCH4III ACNGT 2 cut(s) 399, 469
HpyCH4IV ACGT 3 cut(s) 383, 666, 676
HpyF10VI GCNNNNNNNGC 6 cut(s) 368, 482, 728, 737, 746, 749
HpyF3I CTNAG 5 cut(s) 714, 732, 745, 1186, 1256
HpySE526I ACGT 3 cut(s) 383, 666, 676
Hsp92II CATG 7 cut(s) 278, 598, 841, 940, 1148, 1254, 1306
HspAI GCGC 1 cut(s) 64
KflI GGGWCCC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 6 cut(s) 57, 119, 156, 879, 1312, 1465
LmnI GCTCC 3 cut(s) 102, 1096, 1104
LweI GCATC 3 cut(s) 226, 270, 1024
MaeI CTAG 4 cut(s) 366, 461, 963, 1044
MaeII ACGT 3 cut(s) 383, 666, 676
MaeIII GTNAC 3 cut(s) 384, 1119, 1454
MalI GATC 6 cut(s) 59, 121, 158, 881, 1314, 1467
MbiI CCGCTC 1 cut(s) 148
MboI GATC 6 cut(s) 57, 119, 156, 879, 1312, 1465
MboII GAAGA 6 cut(s) 67, 189, 819, 825, 1019, 1473
MfeI CAATTG 1 cut(s) 759
MhlI GDGCHC 3 cut(s) 107, 676, 1101
MluCI AATT 8 cut(s) 430, 493, 522, 652, 687, 707, 759, 777
MlyI GAGTC 5 cut(s) 121, 908, 1079, 1187, 1379
MmeI TCCRAC 2 cut(s) 68, 1315
Mph1103I ATGCAT 1 cut(s) 540
MroXI GAANNNNTTC 1 cut(s) 1480
MseI TTAA 4 cut(s) 633, 690, 710, 803
MspA1I CMGCKG 1 cut(s) 1076
MspI CCGG 4 cut(s) 78, 357, 1283, 1327
MspR9I CCNGG 4 cut(s) 79, 101, 357, 1284
MunI CAATTG 1 cut(s) 759
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 6 cut(s) 368, 482, 728, 737, 746, 749
NciI CCSGG 3 cut(s) 79, 357, 1284
NcoI CCATGG 2 cut(s) 274, 594
NdeII GATC 6 cut(s) 57, 119, 156, 879, 1312, 1465
NlaIII CATG 7 cut(s) 278, 598, 841, 940, 1148, 1254, 1306
NlaIV GGNNCC 6 cut(s) 105, 165, 223, 316, 317, 592
NmeAIII GCCGAG 1 cut(s) 234
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 4 cut(s) 940, 1148, 1254, 1306
PaeI GCATGC 1 cut(s) 1254
PciI ACATGT 2 cut(s) 936, 1302
PcsI WCGNNNNNNNCGW 1 cut(s) 667
PdmI GAANNNNTTC 1 cut(s) 1480
PfeI GAWTC 7 cut(s) 680, 725, 812, 830, 854, 1028, 1406
Pfl23II CGTACG 1 cut(s) 667
PflMI CCANNNNNTGG 1 cut(s) 587
PleI GAGTC 5 cut(s) 121, 908, 1078, 1186, 1378
PmaCI CACGTG 1 cut(s) 677
PmlI CACGTG 1 cut(s) 677
PpsI GAGTC 5 cut(s) 121, 908, 1078, 1186, 1378
Ppu21I YACGTR 1 cut(s) 677
PpuMI RGGWCCY 1 cut(s) 315
PscI ACATGT 2 cut(s) 936, 1302
Psp124BI GAGCTC 1 cut(s) 1101
Psp1406I AACGTT 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 99
PspCI CACGTG 1 cut(s) 677
PspEI GGTNACC 1 cut(s) 1454
PspGI CCWGG 1 cut(s) 99
PspLI CGTACG 1 cut(s) 667
PspN4I GGNNCC 6 cut(s) 105, 165, 223, 316, 317, 592
PspOMI GGGCCC 1 cut(s) 103
PspPI GGNCC 7 cut(s) 103, 104, 163, 222, 315, 412, 590
PspPPI RGGWCCY 1 cut(s) 315
PstI CTGCAG 2 cut(s) 508, 742
PstNI CAGNNNCTG 1 cut(s) 1037
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 4 cut(s) 405, 466, 669, 1281
RsaNI GTAC 4 cut(s) 404, 465, 668, 1280
SacI GAGCTC 1 cut(s) 1101
SaqAI TTAA 4 cut(s) 633, 690, 710, 803
Sau3AI GATC 6 cut(s) 57, 119, 156, 879, 1312, 1465
Sau96I GGNCC 7 cut(s) 103, 104, 163, 222, 315, 412, 590
SchI GAGTC 5 cut(s) 121, 908, 1079, 1187, 1379
ScrFI CCNGG 4 cut(s) 79, 101, 357, 1284
SduI GDGCHC 3 cut(s) 107, 676, 1101
SfaNI GCATC 3 cut(s) 226, 270, 1024
SfcI CTRYAG 3 cut(s) 504, 738, 1487
SinI GGWCC 4 cut(s) 163, 222, 315, 590
SmlI CTYRAG 2 cut(s) 113, 436
SmoI CTYRAG 2 cut(s) 113, 436
SphI GCATGC 1 cut(s) 1254
Sse9I AATT 8 cut(s) 430, 493, 522, 652, 687, 707, 759, 777
SsiI CCGC 3 cut(s) 133, 148, 320
SspMI CTAG 4 cut(s) 366, 461, 963, 1044
SstI GAGCTC 1 cut(s) 1101
StyD4I CCNGG 4 cut(s) 77, 99, 355, 1282
StyI CCWWGG 2 cut(s) 274, 594
TaaI ACNGT 2 cut(s) 399, 469
TaiI ACGT 3 cut(s) 386, 669, 679
TaqI TCGA 3 cut(s) 795, 1164, 1373
TasI AATT 8 cut(s) 430, 493, 522, 652, 687, 707, 759, 777
TfiI GAWTC 7 cut(s) 680, 725, 812, 830, 854, 1028, 1406
Tru1I TTAA 4 cut(s) 633, 690, 710, 803
Tru9I TTAA 4 cut(s) 633, 690, 710, 803
TscAI CASTG 2 cut(s) 630, 1086
TspDTI ATGAA 2 cut(s) 629, 780
TspRI CASTG 2 cut(s) 630, 1086
Van91I CCANNNNNTGG 1 cut(s) 587
VpaK11BI GGWCC 4 cut(s) 163, 222, 315, 590
XapI RAATTY 3 cut(s) 430, 493, 652
XceI RCATGY 4 cut(s) 940, 1148, 1254, 1306
XmnI GAANNNNTTC 1 cut(s) 1480
XspI CTAG 4 cut(s) 366, 461, 963, 1044
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.