Rh2DG517000

Pleckstrin homology domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012240
Physical Location & Seq
Forward (+)
74048173 .. 74052484
4312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG517000.1

Sequence Viewer

Length: 1419 bp
ATGGCCTCCAATGGCGCTGCTCCGAGAGTTGCAGACCCAGAGAATAGCTTGGAGAAGATCAAGCGCCAGCTCGCTTCCGGTTCGGGTCGGAACTTGCTCCAGGGCCCACTTCTCAAGCGATCCGAGACTCTGCGAAAGTGGAACGAGCGGTGGATGATCTTAGACCCGACGACAGGGAGAATGGAATACAAGATTAGGAGAAATGAGCCGAGTGTGAAGGGAACCATTCTATTTGATGCGAATAGCACAATCGCCCTATCTCCAGTGAACTTTCATGGGTTGGCAAAGTATGATGGCTGCTGTTTCTATATTGGGACCCCGCAGAAAAAAGACTATTTCCTTTGTGCAGAAACTCCTGGTGCGGCCAGAGCTTGGGTATCAACGTTACAAGCAACACAGTTGGTCCTAAAGGCCCATAAAGAAGCTGTGAATTCCTTGAGCGGCAATGGTTCAGCAAAATTAGGAACTGTTGCTGCAGTAGTTGCTGCTGCAAATTCAACTGCTGCAGAGAGTTCTAAGGAAATTGAAGCAGCAATGCATATTTCTTTGAGAAATGCTTTGGGAATGATAACCCAGAAGCCAACTGATGGTCCCATGGATGATTTTGCAGTAATGAAGGAGACACTGAAGGTCAAAGATGAGGAACTACAAAATTTGGCTCGGGACTTACGAGCACGTGATTCAACAATTAAAGAACTTGCAGAAAAATTGACTGAGACTGCTGAATCTGCCGAGGCTGCAGCCTTAGCAGCTCATACAATTGATGAACAAAGGAGAATTGCTTGTGCAGAAATCGAGCGTTTAAGAAGAGATTCAGAGAAGCAGCGGGAATCTTCCAAGTTAAAGGAGGCTCATATGTGGCGTTCTGAACTTGCAAAAGCTAGAGAGCGTGTTGTGATATTAGAAGCAGCCGTTGTGAGAGCCGAAGAGAAGGTCAGGGTTGCAGATGCAGATGCCGAAGCTAGAATAAAGGAGGCTGTTCAGAAAGAATCAGCTGCGCTGAATGAGAAGCAGGAGCTCCATGTCTATGTAAATAAGTTACAAGCACAACTCCAAAGAGAGCACGTTGATATGAAGCAAGTGTTTGAGGAGAAGTCAGAGTCTTGCTCAGATATTGGCAATAGACCCCTGACAAAACATGTAGACTTATCTGAGGAAAATGTTGATAAAGCATGCCTCAGTGTTTCTAGATCGGTTCCAGTACCAGCTGAGAGTGTAGTTCACATGGCAGGGGATCAGGCCAACTTCCGGCCAGTTGGAGATGGTGAGTGGAGTGATATTCAGGCTACAGAGTCGAGGATAGCTGATGTAAGAGAAATCGCCCCCGAGTCAGAAGCAAGCAGCTTAGATATTCCTGTTGTTAGCCAACCAGTTGGTAACCATCATGATCAAGGGGGAAACTCTTTTCAACAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

472

Amino Acids

51.89

Weight (kDa)

5.89

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 33 - 131 7.1e-06 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 372, 587
AccBSI CCGCTC 2 cut(s) 148, 441
AccI GTMKAC 1 cut(s) 1143
AciI CCGC 5 cut(s) 148, 320, 362, 441, 826
AclI AACGTT 1 cut(s) 383
AclWI GGATC 2 cut(s) 114, 1242
AcoI YGGCCR 2 cut(s) 363, 1250
AcsI RAATTY 3 cut(s) 430, 493, 652
AcuI CTGAAG 1 cut(s) 647
AcvI CACGTG 1 cut(s) 677
AfaI GTAC 1 cut(s) 1203
AfiI CCNNNNNNNGG 4 cut(s) 173, 372, 587, 1248
AflIII ACRYGT 1 cut(s) 1138
AgsI TTSAA 4 cut(s) 498, 527, 684, 1409
AjnI CCWGG 2 cut(s) 99, 355
Alw21I GWGCWC 3 cut(s) 676, 1020, 1065
Alw26I GTCTC 3 cut(s) 119, 614, 710
AlwI GGATC 2 cut(s) 114, 1242
Ama87I CYCGRG 2 cut(s) 660, 1325
AoxI GGCC 6 cut(s) 3, 103, 363, 411, 1239, 1250
ApaI GGGCCC 1 cut(s) 107
ApoI RAATTY 3 cut(s) 430, 493, 652
ArsI GACNNNNNNTTYG 2 cut(s) 918, 950
Asp700I GAANNNNTTC 1 cut(s) 811
AspLEI GCGC 3 cut(s) 17, 66, 1000
AspS9I GGNCC 6 cut(s) 103, 104, 315, 403, 412, 590
AsuHPI GGTGA 1 cut(s) 1277
AvaI CYCGRG 2 cut(s) 660, 1325
AvaII GGWCC 3 cut(s) 315, 403, 590
BaeGI GKGCMC 1 cut(s) 107
BanII GRGCYC 2 cut(s) 107, 1020
BbrPI CACGTG 1 cut(s) 677
Bbv12I GWGCWC 3 cut(s) 676, 1020, 1065
BccI CCATC 4 cut(s) 287, 581, 1256, 1389
BceAI ACGGC 1 cut(s) 896
BciT130I CCWGG 2 cut(s) 101, 357
BclI TGATCA 1 cut(s) 1387
BcoDI GTCTC 3 cut(s) 119, 614, 710
BfaI CTAG 3 cut(s) 882, 963, 1188
BfmI CTRYAG 4 cut(s) 474, 504, 738, 1287
BfoI RGCGCY 2 cut(s) 18, 67
Bme1390I CCNGG 2 cut(s) 101, 357
Bme18I GGWCC 3 cut(s) 315, 403, 590
BmeT110I CYCGRG 2 cut(s) 660, 1325
BmgT120I GGNCC 6 cut(s) 103, 104, 315, 403, 412, 590
BmiI GGNNCC 6 cut(s) 105, 223, 316, 317, 592, 1197
BmrFI CCNGG 2 cut(s) 101, 357
BmsI GCATC 3 cut(s) 226, 937, 943
BplI GAGNNNNNCTC 2 cut(s) 1091, 1123
BpmI CTGGAG 2 cut(s) 83, 246
Bpu10I CCTNAGC 1 cut(s) 745
BpuEI CTTGAG 2 cut(s) 98, 457
BsaAI YACGTR 1 cut(s) 677
BsaJI CCNNGG 3 cut(s) 100, 594, 732
BsaWI WCCGGW 1 cut(s) 77
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 372, 587, 1248
Bse1I ACTGG 4 cut(s) 263, 1199, 1253, 1370
Bse3DI GCAATG 2 cut(s) 451, 540
BseBI CCWGG 2 cut(s) 101, 357
BseDI CCNNGG 3 cut(s) 100, 594, 732
BseGI GGATG 2 cut(s) 159, 604
BseLI CCNNNNNNNGG 4 cut(s) 173, 372, 587, 1248
BseMI GCAATG 2 cut(s) 451, 540
BseMII CTCAG 5 cut(s) 705, 1122, 1143, 1192, 1200
BseNI ACTGG 4 cut(s) 263, 1199, 1253, 1370
BseRI GAGGAG 1 cut(s) 1103
BseSI GKGCMC 1 cut(s) 107
BsgI GTGCAG 2 cut(s) 366, 807
BshFI GGCC 6 cut(s) 5, 105, 365, 413, 1241, 1252
BsiHKAI GWGCWC 3 cut(s) 676, 1020, 1065
BsiHKCI CYCGRG 2 cut(s) 660, 1325
BsiSI CCGG 2 cut(s) 78, 1249
BslFI GGGAC 3 cut(s) 328, 576, 677
BslI CCNNNNNNNGG 4 cut(s) 173, 372, 587, 1248
BsmAI GTCTC 3 cut(s) 119, 614, 710
BsmFI GGGAC 3 cut(s) 328, 576, 677
BsnI GGCC 6 cut(s) 5, 105, 365, 413, 1241, 1252
BsoBI CYCGRG 2 cut(s) 660, 1325
Bsp120I GGGCCC 1 cut(s) 103
Bsp1286I GDGCHC 4 cut(s) 107, 676, 1020, 1065
Bsp143I GATC 6 cut(s) 57, 119, 156, 1190, 1234, 1387
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 5 cut(s) 148, 320, 362, 441, 826
BspANI GGCC 6 cut(s) 5, 105, 365, 413, 1241, 1252
BspCNI CTCAG 5 cut(s) 706, 1121, 1144, 1191, 1201
BspHI TCATGA 1 cut(s) 1384
BspLI GGNNCC 6 cut(s) 105, 223, 316, 317, 592, 1197
BspMAI CTGCAG 3 cut(s) 478, 508, 742
BspPI GGATC 2 cut(s) 114, 1242
BsrBI CCGCTC 2 cut(s) 148, 441
BsrDI GCAATG 2 cut(s) 451, 540
BsrI ACTGG 4 cut(s) 263, 1199, 1253, 1370
BssECI CCNNGG 3 cut(s) 100, 594, 732
BssMI GATC 6 cut(s) 57, 119, 156, 1190, 1234, 1387
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 2 cut(s) 101, 357
Bst4CI ACNGT 2 cut(s) 399, 469
Bst6I CTCTTC 2 cut(s) 802, 921
BstAPI GCANNNNNTGC 1 cut(s) 482
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 4 cut(s) 68, 72, 1174, 1339
BstDEI CTNAG 9 cut(s) 160, 516, 714, 745, 1108, 1152, 1178, 1209, 1345
BstDSI CCRYGG 1 cut(s) 594
BstEII GGTNACC 1 cut(s) 1376
BstF5I GGATG 2 cut(s) 159, 604
BstH2I RGCGCY 2 cut(s) 18, 67
BstHHI GCGC 3 cut(s) 17, 66, 1000
BstKTI GATC 6 cut(s) 60, 122, 159, 1193, 1237, 1390
BstMAI GTCTC 3 cut(s) 119, 614, 710
BstMBI GATC 6 cut(s) 57, 119, 156, 1190, 1234, 1387
BstMWI GCNNNNNNNGC 6 cut(s) 368, 482, 728, 737, 746, 749
BstNI CCWGG 2 cut(s) 101, 357
BstNSI RCATGY 2 cut(s) 1142, 1176
BstPI GGTNACC 1 cut(s) 1376
BstSCI CCNGG 2 cut(s) 99, 355
BstSFI CTRYAG 4 cut(s) 474, 504, 738, 1287
BstSLI GKGCMC 1 cut(s) 107
BstXI CCANNNNNNTGG 1 cut(s) 1373
BsuRI GGCC 6 cut(s) 5, 105, 365, 413, 1241, 1252
BtgI CCRYGG 1 cut(s) 594
BtsCI GGATG 2 cut(s) 159, 604
BtsIMutI CAGTG 3 cut(s) 270, 623, 1186
Cac8I GCNNGC 4 cut(s) 68, 72, 1174, 1339
CciI TCATGA 1 cut(s) 1384
CfoI GCGC 3 cut(s) 17, 66, 1000
Cfr13I GGNCC 6 cut(s) 103, 104, 315, 403, 412, 590
Csp6I GTAC 1 cut(s) 1202
CviAII CATG 7 cut(s) 275, 595, 1022, 1139, 1173, 1225, 1385
CviQI GTAC 1 cut(s) 1202
DdeI CTNAG 9 cut(s) 160, 516, 714, 745, 1108, 1152, 1178, 1209, 1345
DpnI GATC 6 cut(s) 59, 121, 158, 1192, 1236, 1389
DpnII GATC 6 cut(s) 57, 119, 156, 1190, 1234, 1387
EaeI YGGCCR 2 cut(s) 363, 1250
Eam1104I CTCTTC 2 cut(s) 802, 921
EarI CTCTTC 2 cut(s) 802, 921
Ecl136II GAGCTC 1 cut(s) 1018
Eco130I CCWWGG 1 cut(s) 594
Eco24I GRGCYC 2 cut(s) 107, 1020
Eco47I GGWCC 3 cut(s) 315, 403, 590
Eco53kI GAGCTC 1 cut(s) 1018
Eco57I CTGAAG 1 cut(s) 647
Eco72I CACGTG 1 cut(s) 677
Eco88I CYCGRG 2 cut(s) 660, 1325
Eco91I GGTNACC 1 cut(s) 1376
EcoICRI GAGCTC 1 cut(s) 1018
EcoO109I RGGNCCY 2 cut(s) 103, 315
EcoO65I GGTNACC 1 cut(s) 1376
EcoRI GAATTC 1 cut(s) 430
EcoRII CCWGG 2 cut(s) 99, 355
EcoT14I CCWWGG 1 cut(s) 594
EcoT22I ATGCAT 1 cut(s) 540
EcoT38I GRGCYC 2 cut(s) 107, 1020
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 7 cut(s) 278, 598, 1025, 1142, 1176, 1228, 1388
FaqI GGGAC 3 cut(s) 328, 576, 677
FatI CATG 7 cut(s) 274, 594, 1021, 1138, 1172, 1224, 1384
FauI CCCGC 2 cut(s) 327, 819
FauNDI CATATG 1 cut(s) 855
FbaI TGATCA 1 cut(s) 1387
FblI GTMKAC 1 cut(s) 1143
FokI GGATG 2 cut(s) 166, 611
FriOI GRGCYC 2 cut(s) 107, 1020
FspBI CTAG 3 cut(s) 882, 963, 1188
GlaI GCGC 3 cut(s) 16, 65, 999
GsuI CTGGAG 2 cut(s) 83, 246
HaeII RGCGCY 2 cut(s) 18, 67
HaeIII GGCC 6 cut(s) 5, 105, 365, 413, 1241, 1252
HapII CCGG 2 cut(s) 78, 1249
HhaI GCGC 3 cut(s) 17, 66, 1000
Hin1II CATG 7 cut(s) 278, 598, 1025, 1142, 1176, 1228, 1388
Hin6I GCGC 3 cut(s) 15, 64, 998
HinP1I GCGC 3 cut(s) 15, 64, 998
HinfI GANTC 9 cut(s) 127, 680, 725, 812, 830, 989, 1100, 1292, 1328
HpaII CCGG 2 cut(s) 78, 1249
HphI GGTGA 1 cut(s) 1277
Hpy166II GTNNAC 3 cut(s) 268, 1144, 1222
Hpy188III TCNNGA 3 cut(s) 662, 1188, 1385
Hpy8I GTNNAC 3 cut(s) 268, 1144, 1222
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 4 cut(s) 211, 610, 622, 925
HpyCH4III ACNGT 2 cut(s) 399, 469
HpyCH4IV ACGT 3 cut(s) 383, 676, 1065
HpyF10VI GCNNNNNNNGC 6 cut(s) 368, 482, 728, 737, 746, 749
HpyF3I CTNAG 9 cut(s) 160, 516, 714, 745, 1108, 1152, 1178, 1209, 1345
HpySE526I ACGT 3 cut(s) 383, 676, 1065
Hsp92II CATG 7 cut(s) 278, 598, 1025, 1142, 1176, 1228, 1388
HspAI GCGC 3 cut(s) 15, 64, 998
KflI GGGWCCC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 1387
Kzo9I GATC 6 cut(s) 57, 119, 156, 1190, 1234, 1387
LmnI GCTCC 4 cut(s) 25, 102, 1015, 1023
LweI GCATC 3 cut(s) 226, 937, 943
MaeI CTAG 3 cut(s) 882, 963, 1188
MaeII ACGT 3 cut(s) 383, 676, 1065
MaeIII GTNAC 3 cut(s) 384, 1038, 1376
MalI GATC 6 cut(s) 59, 121, 158, 1192, 1236, 1389
MbiI CCGCTC 2 cut(s) 148, 441
MboI GATC 6 cut(s) 57, 119, 156, 1190, 1234, 1387
MboII GAAGA 4 cut(s) 67, 819, 825, 938
MfeI CAATTG 1 cut(s) 759
MhlI GDGCHC 4 cut(s) 107, 676, 1020, 1065
MluCI AATT 9 cut(s) 430, 458, 493, 522, 652, 687, 707, 759, 777
MlyI GAGTC 4 cut(s) 121, 1109, 1301, 1337
MmeI TCCRAC 2 cut(s) 68, 1237
MnlI CCTC 9 cut(s) 16, 634, 727, 841, 967, 1081, 1147, 1187, 1290
Mph1103I ATGCAT 1 cut(s) 540
MroXI GAANNNNTTC 1 cut(s) 811
MseI TTAA 3 cut(s) 690, 803, 842
MslI CAYNNNNRTG 1 cut(s) 1026
MspA1I CMGCKG 3 cut(s) 826, 995, 1208
MspI CCGG 2 cut(s) 78, 1249
MspR9I CCNGG 2 cut(s) 101, 357
MunI CAATTG 1 cut(s) 759
MvaI CCWGG 2 cut(s) 101, 357
MwoI GCNNNNNNNGC 6 cut(s) 368, 482, 728, 737, 746, 749
NcoI CCATGG 1 cut(s) 594
NdeI CATATG 1 cut(s) 855
NdeII GATC 6 cut(s) 57, 119, 156, 1190, 1234, 1387
NlaIII CATG 7 cut(s) 278, 598, 1025, 1142, 1176, 1228, 1388
NlaIV GGNNCC 6 cut(s) 105, 223, 316, 317, 592, 1197
NmeAIII GCCGAG 2 cut(s) 234, 757
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 2 cut(s) 1142, 1176
PaeI GCATGC 1 cut(s) 1176
PagI TCATGA 1 cut(s) 1384
PciI ACATGT 1 cut(s) 1138
PcsI WCGNNNNNNNCGW 1 cut(s) 667
PdmI GAANNNNTTC 1 cut(s) 811
PfeI GAWTC 5 cut(s) 680, 725, 812, 830, 989
PflMI CCANNNNNTGG 2 cut(s) 372, 587
PleI GAGTC 4 cut(s) 121, 1108, 1300, 1336
PmaCI CACGTG 1 cut(s) 677
PmlI CACGTG 1 cut(s) 677
PpsI GAGTC 4 cut(s) 121, 1108, 1300, 1336
Ppu21I YACGTR 1 cut(s) 677
PpuMI RGGWCCY 1 cut(s) 315
PscI ACATGT 1 cut(s) 1138
Psp124BI GAGCTC 1 cut(s) 1020
Psp1406I AACGTT 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 99, 355
PspCI CACGTG 1 cut(s) 677
PspEI GGTNACC 1 cut(s) 1376
PspGI CCWGG 2 cut(s) 99, 355
PspN4I GGNNCC 6 cut(s) 105, 223, 316, 317, 592, 1197
PspOMI GGGCCC 1 cut(s) 103
PspPI GGNCC 6 cut(s) 103, 104, 315, 403, 412, 590
PspPPI RGGWCCY 1 cut(s) 315
PstI CTGCAG 3 cut(s) 478, 508, 742
PvuII CAGCTG 2 cut(s) 995, 1208
RsaI GTAC 1 cut(s) 1203
RsaNI GTAC 1 cut(s) 1202
RseI CAYNNNNRTG 1 cut(s) 1026
SacI GAGCTC 1 cut(s) 1020
SaqAI TTAA 3 cut(s) 690, 803, 842
Sau3AI GATC 6 cut(s) 57, 119, 156, 1190, 1234, 1387
Sau96I GGNCC 6 cut(s) 103, 104, 315, 403, 412, 590
SchI GAGTC 4 cut(s) 121, 1109, 1301, 1337
ScrFI CCNGG 2 cut(s) 101, 357
SduI GDGCHC 4 cut(s) 107, 676, 1020, 1065
SfaNI GCATC 3 cut(s) 226, 937, 943
SfcI CTRYAG 4 cut(s) 474, 504, 738, 1287
SinI GGWCC 3 cut(s) 315, 403, 590
SmiMI CAYNNNNRTG 1 cut(s) 1026
SmlI CTYRAG 2 cut(s) 113, 436
SmoI CTYRAG 2 cut(s) 113, 436
SphI GCATGC 1 cut(s) 1176
Sse9I AATT 9 cut(s) 430, 458, 493, 522, 652, 687, 707, 759, 777
SsiI CCGC 5 cut(s) 148, 320, 362, 441, 826
SspMI CTAG 3 cut(s) 882, 963, 1188
SstI GAGCTC 1 cut(s) 1020
StyD4I CCNGG 2 cut(s) 99, 355
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 2 cut(s) 399, 469
TaiI ACGT 3 cut(s) 386, 679, 1068
TaqI TCGA 2 cut(s) 795, 1295
TasI AATT 9 cut(s) 430, 458, 493, 522, 652, 687, 707, 759, 777
TauI GCSGC 2 cut(s) 365, 444
TfiI GAWTC 5 cut(s) 680, 725, 812, 830, 989
Tru1I TTAA 3 cut(s) 690, 803, 842
Tru9I TTAA 3 cut(s) 690, 803, 842
TscAI CASTG 3 cut(s) 270, 630, 1186
TspDTI ATGAA 4 cut(s) 263, 629, 780, 1088
TspRI CASTG 3 cut(s) 270, 630, 1186
Van91I CCANNNNNTGG 2 cut(s) 372, 587
VpaK11BI GGWCC 3 cut(s) 315, 403, 590
XapI RAATTY 3 cut(s) 430, 493, 652
XbaI TCTAGA 1 cut(s) 1187
XceI RCATGY 2 cut(s) 1142, 1176
XmiI GTMKAC 1 cut(s) 1143
XmnI GAANNNNTTC 1 cut(s) 811
XspI CTAG 3 cut(s) 882, 963, 1188
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.