MD09G1148200.v1.1

Pleckstrin homology domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012240
Physical Location & Seq
Reverse (-)
11656846 .. 11660879
4034 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1148200.v1.1.491

Sequence Viewer

Length: 1425 bp
ATGGCCACCACCAATGGCGCCTCTCCGAGAGCTGCGGACGCTGAGAACAGCTTGGAGAAGATCAAGCGCCAGCTCGCATCAGGTTCGGGTCGGAACTTGTTGCAGGGTCCACTTCTCAAGCGATCCGAGACTCTGAGAAAATGGAATGAGCGGTGGGTGATCTTAGACCCGACGACAGGGAGAATGGAATACAAGATTAGGAGAAATGAGCCGACTGTCAAGGGAACCATTCCATTTGATGCGAATAGCACAATCACCCTATCTCCCGTAAACTTCCATGGGTTGCAAAAGTATGACGGTTGCTGTTTCTATATTGGGACCCCGCAGAAAAAGGACTACTTCCTTTGTGCAGAGACTCCTGGGGCAGCTAGAGCTTGGGTAGCAACTCTACAAGCAACACAATTGGTTCTAAAGGCCCATAAAGAAGCTGTGAATTCCTTGAGTGGCAATGGCTCTGCAAAACTAGGAACGGTTGCAGCAGTAGTTGCTGCTGCCAATTCAACTGCTTCGGAGAGTTCTAAGGAAATCGAAGCAGCAATGCACATTTCTTTGAGAAATGCTTTGGGAGTGATAACCAATAAGCCAACTGATGGGCCAATGGATGATTTTGCAATTATGAAGGAGACACTTCGGGTCAAAGATGACGAACTACATAATTTGGCTAGGGACCTTCGTTCACGCGATTCAACAATTAAAGAAATAGCAGATAAATTAACTGAGACTGCCGAAGCTGCTGAGGCTGCAGCCTCTGCAGCTCATACAATTGATGAACAAAGGAAAATTGTTTGTGCAGAAATTGATCGCTTAAAAAAGGATTCAGCGAAGCAGCTGGAATCTTCCTTGTCAAAGCTAAAAGATTCTGAAGAAAAGGTTGCGACCTTGAGCAAAGAAAGAGACCAATTGACTAGGCAGAGAGATTCTGCTCTCCAAGAGGCACACATGTGGCGTTCTGAGCTCGCAAAAGCTAGAGAGCGTGTTGTGATATTAGAAGCAGCTGTTGTGAGGGCAGAGGAGAAGGTCAGGGTTGCCGAGGCAGATGCTCAAGCTAGAATAAAGGAGGCTGTGCAGAAAGAATCAGCCGCGCTGAAACACAAGCAAGAGCTTCTGGAATATGTGAATACGTTGCAAGCACAACTTCAGAGGCATCACATAGATACAAAGCAAGTGTTCGAGAAGTCTGAGTCTTGCTCAGATATTGGAAATACACCCTTGACTAAACATGTAGACTTGTCTGAAGAAAATGTTGATAAAGCATGCCTCAGTGTTTCTAGAGCAGTTCCGGTACCAGGGGAGAGTGTAGTCCACATGGCTGACGGTCAAGTCAACCTACAGCCAGTTGGTGACCGTGAATGGAGTGACATTCAGGCTCAAGAGGCAAGGATAGCCGACGTAAGAGAAGTCGACCCCCCAACCAGAAGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

475

Amino Acids

52.21

Weight (kDa)

6.63

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 34 - 132 5.6e-07 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1319
Acc65I GGTACC 1 cut(s) 1282
AccB1I GGYRCC 2 cut(s) 17, 1282
AccB7I CCANNNNNTGG 1 cut(s) 590
AccBSI CCGCTC 1 cut(s) 151
AccI GTMKAC 2 cut(s) 1224, 1401
AccII CGCG 2 cut(s) 681, 1082
AciI CCGC 4 cut(s) 35, 151, 323, 1080
AclWI GGATC 1 cut(s) 117
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 3 cut(s) 882, 1121, 1254
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 1 cut(s) 1284
AfiI CCNNNNNNNGG 3 cut(s) 176, 590, 1286
AflIII ACRYGT 2 cut(s) 939, 1219
AgsI TTSAA 2 cut(s) 501, 687
AjnI CCWGG 2 cut(s) 358, 1285
AleI CACNNNNGTG 1 cut(s) 940
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 5 cut(s) 122, 347, 617, 713, 888
AlwI GGATC 1 cut(s) 117
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 3 cut(s) 3, 414, 593
ApoI RAATTY 1 cut(s) 433
Asp718I GGTACC 1 cut(s) 1282
AspLEI GCGC 3 cut(s) 20, 69, 1084
AspS9I GGNCC 5 cut(s) 107, 318, 415, 593, 667
AsuHPI GGTGA 3 cut(s) 169, 247, 1352
AvaII GGWCC 3 cut(s) 107, 318, 667
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 17, 1282
BanII GRGCYC 1 cut(s) 957
Bbv12I GWGCWC 1 cut(s) 957
BbvCI CCTCAGC 1 cut(s) 735
BccI CCATC 1 cut(s) 584
BcgI CGANNNNNNTGC 4 cut(s) 66, 100, 1019, 1053
BciT130I CCWGG 2 cut(s) 360, 1287
BcoDI GTCTC 5 cut(s) 122, 347, 617, 713, 888
BfaI CTAG 7 cut(s) 369, 464, 663, 906, 966, 1047, 1269
BfmI CTRYAG 3 cut(s) 741, 750, 1328
BfoI RGCGCY 2 cut(s) 21, 70
Bme1390I CCNGG 2 cut(s) 360, 1287
Bme18I GGWCC 3 cut(s) 107, 318, 667
BmgT120I GGNCC 5 cut(s) 107, 318, 415, 593, 667
BmiI GGNNCC 7 cut(s) 19, 108, 226, 319, 320, 668, 1284
BmrFI CCNGG 2 cut(s) 360, 1287
BmsI GCATC 4 cut(s) 86, 229, 1027, 1153
BplI GAGNNNNNCTC 2 cut(s) 1172, 1204
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 5 cut(s) 101, 460, 901, 1026, 1353
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 888
BsaJI CCNNGG 4 cut(s) 277, 359, 1029, 1286
BsaWI WCCGGW 1 cut(s) 1279
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 590, 1286
Bse1I ACTGG 1 cut(s) 1334
Bse3DI GCAATG 2 cut(s) 454, 543
BseBI CCWGG 2 cut(s) 360, 1287
BseDI CCNNGG 4 cut(s) 277, 359, 1029, 1286
BseGI GGATG 1 cut(s) 607
BseLI CCNNNNNNNGG 3 cut(s) 176, 590, 1286
BseMI GCAATG 2 cut(s) 454, 543
BseMII CTCAG 8 cut(s) 33, 125, 708, 726, 942, 1170, 1203, 1273
BseNI ACTGG 1 cut(s) 1334
BseRI GAGGAG 1 cut(s) 1025
BsgI GTGCAG 3 cut(s) 369, 810, 1085
Bsh1236I CGCG 2 cut(s) 681, 1082
BshFI GGCC 3 cut(s) 5, 416, 595
BshNI GGYRCC 2 cut(s) 17, 1282
BsiHKAI GWGCWC 1 cut(s) 957
BsiSI CCGG 1 cut(s) 1280
BslFI GGGAC 2 cut(s) 331, 680
BslI CCNNNNNNNGG 3 cut(s) 176, 590, 1286
BsmAI GTCTC 5 cut(s) 122, 347, 617, 713, 888
BsmFI GGGAC 2 cut(s) 331, 680
BsnI GGCC 3 cut(s) 5, 416, 595
Bso31I GGTCTC 1 cut(s) 888
Bsp1286I GDGCHC 1 cut(s) 957
Bsp143I GATC 4 cut(s) 60, 122, 159, 799
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 4 cut(s) 35, 151, 323, 1080
BspANI GGCC 3 cut(s) 5, 416, 595
BspCNI CTCAG 8 cut(s) 34, 126, 709, 727, 943, 1171, 1202, 1272
BspFNI CGCG 2 cut(s) 681, 1082
BspLI GGNNCC 7 cut(s) 19, 108, 226, 319, 320, 668, 1284
BspMAI CTGCAG 2 cut(s) 745, 754
BspPI GGATC 1 cut(s) 117
BspT107I GGYRCC 2 cut(s) 17, 1282
BspTNI GGTCTC 1 cut(s) 888
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 2 cut(s) 454, 543
BsrI ACTGG 1 cut(s) 1334
BssECI CCNNGG 4 cut(s) 277, 359, 1029, 1286
BssMI GATC 4 cut(s) 60, 122, 159, 799
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 2 cut(s) 360, 1287
Bst4CI ACNGT 5 cut(s) 217, 299, 472, 1316, 1346
BstACI GRCGYC 1 cut(s) 18
BstAPI GCANNNNNTGC 2 cut(s) 485, 749
BstC8I GCNNGC 5 cut(s) 71, 75, 957, 1128, 1255
BstDSI CCRYGG 1 cut(s) 277
BstEII GGTNACC 1 cut(s) 1340
BstF5I GGATG 1 cut(s) 607
BstFNI CGCG 2 cut(s) 681, 1082
BstH2I RGCGCY 2 cut(s) 21, 70
BstHHI GCGC 3 cut(s) 20, 69, 1084
BstKTI GATC 4 cut(s) 63, 125, 162, 802
BstMAI GTCTC 5 cut(s) 122, 347, 617, 713, 888
BstMBI GATC 4 cut(s) 60, 122, 159, 799
BstNI CCWGG 2 cut(s) 360, 1287
BstNSI RCATGY 3 cut(s) 943, 1223, 1257
BstPI GGTNACC 1 cut(s) 1340
BstSCI CCNGG 2 cut(s) 358, 1285
BstSFI CTRYAG 3 cut(s) 741, 750, 1328
BstUI CGCG 2 cut(s) 681, 1082
BsuRI GGCC 3 cut(s) 5, 416, 595
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 1 cut(s) 607
BtsIMutI CAGTG 1 cut(s) 1267
Cac8I GCNNGC 5 cut(s) 71, 75, 957, 1128, 1255
CaiI CAGNNNCTG 1 cut(s) 749
CfoI GCGC 3 cut(s) 20, 69, 1084
Cfr13I GGNCC 5 cut(s) 107, 318, 415, 593, 667
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 1283
CviAII CATG 5 cut(s) 278, 940, 1220, 1254, 1306
CviQI GTAC 1 cut(s) 1283
DinI GGCGCC 1 cut(s) 19
DpnI GATC 4 cut(s) 62, 124, 161, 801
DpnII GATC 4 cut(s) 60, 122, 159, 799
DrdI GACNNNNNNGTC 1 cut(s) 1319
DseDI GACNNNNNNGTC 1 cut(s) 1319
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 955
Eco130I CCWWGG 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 957
Eco31I GGTCTC 1 cut(s) 888
Eco47I GGWCC 3 cut(s) 107, 318, 667
Eco53kI GAGCTC 1 cut(s) 955
Eco57I CTGAAG 3 cut(s) 882, 1121, 1254
Eco91I GGTNACC 1 cut(s) 1340
EcoICRI GAGCTC 1 cut(s) 955
EcoO109I RGGNCCY 2 cut(s) 318, 667
EcoO65I GGTNACC 1 cut(s) 1340
EcoRI GAATTC 1 cut(s) 433
EcoRII CCWGG 2 cut(s) 358, 1285
EcoT14I CCWWGG 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 957
EgeI GGCGCC 1 cut(s) 19
EheI GGCGCC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 5 cut(s) 281, 943, 1223, 1257, 1309
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 1119, 1151
FaqI GGGAC 2 cut(s) 331, 680
FatI CATG 5 cut(s) 277, 939, 1219, 1253, 1305
FauI CCCGC 1 cut(s) 330
FblI GTMKAC 2 cut(s) 1224, 1401
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 957
FspBI CTAG 7 cut(s) 369, 464, 663, 906, 966, 1047, 1269
GlaI GCGC 3 cut(s) 19, 68, 1083
HaeII RGCGCY 2 cut(s) 21, 70
HaeIII GGCC 3 cut(s) 5, 416, 595
HapII CCGG 1 cut(s) 1280
HgaI GACGC 1 cut(s) 47
HhaI GCGC 3 cut(s) 20, 69, 1084
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 5 cut(s) 281, 943, 1223, 1257, 1309
Hin6I GCGC 3 cut(s) 18, 67, 1082
HinP1I GCGC 3 cut(s) 18, 67, 1082
HincII GTYRAC 2 cut(s) 1324, 1402
HindII GTYRAC 2 cut(s) 1324, 1402
HinfI GANTC 9 cut(s) 130, 355, 683, 815, 833, 857, 917, 1073, 1181
HpaII CCGG 1 cut(s) 1280
HphI GGTGA 3 cut(s) 169, 247, 1352
Hpy166II GTNNAC 7 cut(s) 110, 271, 677, 1225, 1303, 1324, 1402
Hpy188III TCNNGA 4 cut(s) 1106, 1171, 1269, 1370
Hpy8I GTNNAC 7 cut(s) 110, 271, 677, 1225, 1303, 1324, 1402
Hpy99I CGWCG 2 cut(s) 175, 1391
HpyAV CCTTC 3 cut(s) 613, 680, 1009
HpyCH4III ACNGT 5 cut(s) 217, 299, 472, 1316, 1346
HpyCH4IV ACGT 2 cut(s) 1121, 1389
HpySE526I ACGT 2 cut(s) 1121, 1389
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 5 cut(s) 281, 943, 1223, 1257, 1309
HspAI GCGC 3 cut(s) 18, 67, 1082
KasI GGCGCC 1 cut(s) 17
KflI GGGWCCC 1 cut(s) 318
KpnI GGTACC 1 cut(s) 1286
Kzo9I GATC 4 cut(s) 60, 122, 159, 799
LweI GCATC 4 cut(s) 86, 229, 1027, 1153
MaeI CTAG 7 cut(s) 369, 464, 663, 906, 966, 1047, 1269
MaeII ACGT 2 cut(s) 1121, 1389
MaeIII GTNAC 2 cut(s) 1340, 1355
MalI GATC 4 cut(s) 62, 124, 161, 801
MbiI CCGCTC 1 cut(s) 151
MboI GATC 4 cut(s) 60, 122, 159, 799
MboII GAAGA 4 cut(s) 70, 828, 875, 1247
MfeI CAATTG 3 cut(s) 401, 762, 899
MhlI GDGCHC 1 cut(s) 957
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 18
MlyI GAGTC 3 cut(s) 124, 349, 1190
MmeI TCCRAC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 693, 713, 806
MslI CAYNNNNRTG 1 cut(s) 940
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 829, 995
MspI CCGG 1 cut(s) 1280
MspR9I CCNGG 2 cut(s) 360, 1287
MunI CAATTG 3 cut(s) 401, 762, 899
MvaI CCWGG 2 cut(s) 360, 1287
MvnI CGCG 2 cut(s) 681, 1082
NarI GGCGCC 1 cut(s) 18
NcoI CCATGG 1 cut(s) 277
NdeII GATC 4 cut(s) 60, 122, 159, 799
NlaIII CATG 5 cut(s) 281, 943, 1223, 1257, 1309
NlaIV GGNNCC 7 cut(s) 19, 108, 226, 319, 320, 668, 1284
NmeAIII GCCGAG 1 cut(s) 1054
NmuCI GTSAC 2 cut(s) 1340, 1355
NspI RCATGY 3 cut(s) 943, 1223, 1257
OliI CACNNNNGTG 1 cut(s) 940
PaeI GCATGC 1 cut(s) 1257
PciI ACATGT 2 cut(s) 939, 1219
PfeI GAWTC 6 cut(s) 683, 815, 833, 857, 917, 1073
PflMI CCANNNNNTGG 1 cut(s) 590
PleI GAGTC 3 cut(s) 124, 349, 1189
PluTI GGCGCC 1 cut(s) 21
PpsI GAGTC 3 cut(s) 124, 349, 1189
PpuMI RGGWCCY 2 cut(s) 318, 667
PscI ACATGT 2 cut(s) 939, 1219
Psp124BI GAGCTC 1 cut(s) 957
Psp5II RGGWCCY 2 cut(s) 318, 667
Psp6I CCWGG 2 cut(s) 358, 1285
PspEI GGTNACC 1 cut(s) 1340
PspGI CCWGG 2 cut(s) 358, 1285
PspN4I GGNNCC 7 cut(s) 19, 108, 226, 319, 320, 668, 1284
PspPI GGNCC 5 cut(s) 107, 318, 415, 593, 667
PspPPI RGGWCCY 2 cut(s) 318, 667
PstI CTGCAG 2 cut(s) 745, 754
PstNI CAGNNNCTG 1 cut(s) 749
PvuII CAGCTG 2 cut(s) 829, 995
RsaI GTAC 1 cut(s) 1284
RsaNI GTAC 1 cut(s) 1283
RseI CAYNNNNRTG 1 cut(s) 940
SacI GAGCTC 1 cut(s) 957
SalI GTCGAC 1 cut(s) 1400
SaqAI TTAA 3 cut(s) 693, 713, 806
Sau3AI GATC 4 cut(s) 60, 122, 159, 799
Sau96I GGNCC 5 cut(s) 107, 318, 415, 593, 667
SchI GAGTC 3 cut(s) 124, 349, 1190
ScrFI CCNGG 2 cut(s) 360, 1287
SduI GDGCHC 1 cut(s) 957
SfaNI GCATC 4 cut(s) 86, 229, 1027, 1153
SfcI CTRYAG 3 cut(s) 741, 750, 1328
SfoI GGCGCC 1 cut(s) 19
SinI GGWCC 3 cut(s) 107, 318, 667
SmiMI CAYNNNNRTG 1 cut(s) 940
SmlI CTYRAG 5 cut(s) 116, 439, 880, 1041, 1368
SmoI CTYRAG 5 cut(s) 116, 439, 880, 1041, 1368
SphI GCATGC 1 cut(s) 1257
SsiI CCGC 4 cut(s) 35, 151, 323, 1080
SspDI GGCGCC 1 cut(s) 17
SspMI CTAG 7 cut(s) 369, 464, 663, 906, 966, 1047, 1269
SstI GAGCTC 1 cut(s) 957
StyD4I CCNGG 2 cut(s) 358, 1285
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 5 cut(s) 217, 299, 472, 1316, 1346
TaiI ACGT 2 cut(s) 1124, 1392
TaqI TCGA 3 cut(s) 528, 1170, 1401
TauI GCSGC 1 cut(s) 1082
TfiI GAWTC 6 cut(s) 683, 815, 833, 857, 917, 1073
Tru1I TTAA 3 cut(s) 693, 713, 806
Tru9I TTAA 3 cut(s) 693, 713, 806
TscAI CASTG 1 cut(s) 1267
TseFI GTSAC 2 cut(s) 1340, 1355
Tsp45I GTSAC 2 cut(s) 1340, 1355
TspDTI ATGAA 2 cut(s) 632, 783
TspRI CASTG 1 cut(s) 1267
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 3 cut(s) 107, 318, 667
XapI RAATTY 1 cut(s) 433
XbaI TCTAGA 1 cut(s) 1268
XceI RCATGY 3 cut(s) 943, 1223, 1257
XmiI GTMKAC 2 cut(s) 1224, 1401
XspI CTAG 7 cut(s) 369, 464, 663, 906, 966, 1047, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.