FvH4_6g40910

Vacuolar protein-sorting-associated protein 11 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012419
Physical Location & Seq
Forward (+)
32338947 .. 32344565
5619 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g40910.t2

Sequence Viewer

Length: 2838 bp
ATGTACCAGTGGCGGAAATTCGAGTTCTTCGAGGAGAAATTGGCGGGAAAGTGCGCGATTCCCGAGGAAGTAAGCGGCAAGATCGAGTGCTGCTCCAGCGGCCGAGGGAAGGTGGTGATCGGCTGCGACGACGGCTCCGTCAGCTTCCTCGACCGCAGCCTCCACTTCAGCTACGGCTTCCAAGCTCACTCCTCCGTTCTCTTCCTTCAGCAGCTCAAGCAAAGGAACTTTCTGGTGACGATTGGAGACGATGAGCAAATCTCGTCGCAGCAATCGAGGTGCTTGAAGGTGTTTGATCTAGATAGGATACAGCCGGAAGGGACTAGCTCGACGAGCCCCGATTGCATTGGTATCTTGAGGATTTTTACTAACCAATTTCCGGAAGCTAAGATTACGTCGTTTTTAGTACTAGAGGAAGCGCCTCCGATATTGCTTATAGCTATTGGATTAGATAATGGCTGCATTTACTGCATTAAAGGGGATATTGCACGGGAGCGTATCACGCGGTTCAAGCTCCAGGTGAATAATGTTTCTGACAAGAGCCAGTGCGCGATCACTGGATTGGGATTCAGAGTGGATGGTCAAGCTCTTCAGTTGTTTGCTGTGACACCGTCTTCTGTGAGCTTGTTCATCTTGCAGAATCAGCCGTCAAGGGGGCAAACATTGGATCAGATTGGAGGCAATGTTAACAGTGTTGCCATGAGTGATCGCGGGGAGTTGATAATTGGTCGACCTGAGGCTGTGTATTTTTATGAAGTTGATGGGCGTGGTCCTTGTTGGGCTTTTGAAGGAGAAAAGAAGTTTTTAGGGTGGTTCCGTGGATATCTTCTGTGCATTATTGAAGATCAAAGAAGTGGTAACCACACTTTCAACATATATGACCTGAAGAACCATTTGATTGCCCACAGCCTTGTGGTGAAAGAGGTTTCACACCTGCTCTGTGAATGGGGTAACATAATACTTATAATGGCTGATAAGTCCGCTTTGTGCATCGGGGAGAAGGATATGGAGAGCAAGTTAGATATGCTTTTTAAGAAGAATCTATATACAGTTGCCATCAACCTTGTTCAGAGTCAGCAAGCTGATGCTGCTGCAACTGCTGAAGTGCTGAGAAAGTATGGTGATCATCTATATAGCAAGCAAGATTATGATGAGGCAATGGCGCAGTATATCCATACCATTGGTCACCTTGAGCCGTCTTATGTTATACAAAAGTTTCTAGATGCACAACGAATCTACAACCTTACTAATTACTTGGAAAAATTACACGAGAAGGGGCTTGCTTCTAAAGATCATACCACTCTTCTATTGAACTGCTACACCAAATTGAAAGATGTTGACAAGCTAAACGTGTTCATCAAAAGTGAGGATGGTTTTGGGGAACATAAGTTTGATGTCGAGACGGCAATACGGGTCTGCCGCGCTACCAACTACCATGAACATGCTATGTATGTCGCTAAGAAGGCAGGGAAGCATGAGTGGTACTTGAAGATCTTACTTGAAGACCTTGGCAGATATGAGGAAGCCTTGCAATATATTTCAAGCCTTGAGCCAAGCCAAGCAGGAGTAACAGTCAAAGAGTATGGTAAGATTCTCATAGAGCACAAGCCCGTGGAGACAATTGAGATACTTATGAGGCTGTGCACCGAGGATGGAGAATCAGCCAAGAGAGGAGGTGCTAATGGTGCATACTTAACTATGTTACCATCTCCTGTTGATTTCCTCAACATCTTCATCCATCATCTTCAGTCGCTTATGGTTTTCCTTGAAAAGTATACCAACAAGGTCAAAGACTCTCCTGCTCAAGTAGAAATCCATAACACACTCTTGGAGTTGTATTTGTCAAATGACTTGAACTTCCCATTGATGTCACAAGCTAGCAATGGTGGTGAAATTAGTGTTAGGTCTACTAGACCAGGAGCAGGGGCAATGTCCAATGGGAAGTTTGTTGCTGATGGCAAAGACTTAACTCAGGAAATGGACCGTATGGAAAGGCAAGAGAAAGGGCTCAGGCTGCTTAAGAGTGCATGGCCGTCTGAGCTGGAACATCCGCTATATGATGTTGATCTTGCTATAATCCTCTGTGAAATGAATGACTTTAAGGAAGGGCTTCTCTATATATATGAGAAGATGAAACTCTACAAAGAGGTGATTTCTTGCTACATGCAAGCCCATGACCATGAAGGTTTGATTTCATGCTGCAAACGATTAGGGGATTCAGGTAAGGGTGGGGACCCAACTCTTTGGGCAGATCTGTTGAAATATTTTGGTGAACTTGGAGAAGATTGTTCCAAAGAAGTGAAGGAAGTTTTGACTTATATAGAAAGGGATGATATCTTGCCTCCTATTATTGTTCTTCAAACACTGTCCAAAAATCCATGCCTCACACTCTCTGTCATTAAGGATTACATTGCTCGAAAGCTTGAACAAGAATCAAAACTGATTGAAGAGGACCGTCGATCTATTGAGAAGTATCAGGAAGCAACATTGACAATGAGAAAAGAAATTCAGGATCTCAGGACAAATGCAAGGATCTTTCAGCTCAGCAAGTGCACCGCATGTACTTTCACCCTTGATCTCCCTGCTGTACATTTTATGTGTATCCACTCATTCCATCAGCGTTGCCTCGGTGATAATGAGAAAGAATGCCCTGTATGTGCTCCTGAATACAGATCTGTTCTAGAATTGAAAACAAGTTTAGAGCAAAACTCTAAAGATCAGGATCGGTTCTTCCAGCAAGTTAAAAGTTCAAAGGATGGGTTTTCTGTAATCGCTGAGTATTTCGGGAAGGGGGTCATTAGCAAAACCAGTAATGGACCCACAGGTGCTTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000323 GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005768 GO:0005769 GO:0005770 GO:0005773 GO:0005774 GO:0005856 GO:0005884 GO:0006810 GO:0006887 GO:0006904 GO:0006906 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0008150 GO:0008333 GO:0009705 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010256 GO:0010604 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019222 GO:0019904 GO:0019905 GO:0022406 GO:0030139 GO:0030674 GO:0030897 GO:0031090 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031338 GO:0031410 GO:0031982 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032879 GO:0032940 GO:0032991 GO:0033043 GO:0033263 GO:0034058 GO:0035542 GO:0042144 GO:0042176 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046903 GO:0046907 GO:0048278 GO:0048284 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051049 GO:0051050 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051640 GO:0051641 GO:0051649 GO:0060090 GO:0060255 GO:0060341 GO:0060627 GO:0061024 GO:0061025 GO:0065007 GO:0071840 GO:0080090 GO:0080171 GO:0090174 GO:0097576 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140029 GO:0140056 GO:1901998 GO:1902115 GO:1903362 GO:1903364 GO:1903649 GO:1903651 GO:2000641 GO:2000643
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin VPS11_C zf-C3H2C3

Protein Analysis

946

Amino Acids

106.86

Weight (kDa)

5.72

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEP5_VPS11_N PF23341 4 - 333 3.7e-90 PEP5/VPS11 N-terminal
VPS11_N PF23266 25 - 328 1.8e-116 VPS11-like, N-terminal beta-propeller
TPR_PEP5_VPS11 PF23356 378 - 618 1e-61 PEP5/VPS11 Clathrin repeat
Clathrin PF00637 398 - 537 1.6e-06 Region in Clathrin and VPS
TPR_PEP5_VPS11 PF23356 683 - 787 1.4e-17 PEP5/VPS11 Clathrin repeat
zf-C3H2C3 PF17122 851 - 886 6.7e-06 Zinc-finger
VPS11_C PF12451 891 - 933 2.1e-13 Vacuolar protein sorting protein 11 C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012419)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05170
fragaria_vesca FvH4_6g40910 FvH4_6g40910
malus_domestica MD09G1123500.v1.1 MD17G1113900.v1.1
prunus_persica Prupe.3G200600_v2.0.a1
pyrus_communis pycom09g04750 pycom17g10560
rosa_chinensis RchiOBHm_Chr2g0156491
rosa_laevigata RLG00000020917
rosa_multiflora Rmu_co8501807.1_g000001 Rmu_sc0005392.1_g000009
rosa_roxburghii Rroxscaffold_2G00092980
rosa_rugosa Rorug02G0453700
rosa_samantha Rh2AG519800 Rh2BG532100 Rh2CG504800 Rh2DG541100
rosa_wichuraiana Rw2G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 965
AarI CACCTGC 1 cut(s) 942
AasI GACNNNNNNGTC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 942
AccI GTMKAC 3 cut(s) 730, 1775, 1907
AccII CGCG 5 cut(s) 56, 505, 551, 711, 1422
AccIII TCCGGA 1 cut(s) 379
AclWI GGATC 4 cut(s) 675, 2520, 2540, 2730
AcoI YGGCCR 2 cut(s) 100, 2030
AcsI RAATTY 2 cut(s) 17, 2505
AcuI CTGAAG 6 cut(s) 151, 191, 575, 905, 1122, 1730
AfaI GTAC 5 cut(s) 5, 408, 1484, 2563, 2589
AfiI CCNNNNNNNGG 4 cut(s) 109, 379, 653, 1922
AflII CTTAAG 1 cut(s) 2018
AflIII ACRYGT 1 cut(s) 1350
AjnI CCWGG 2 cut(s) 516, 1915
Alw21I GWGCWC 4 cut(s) 1605, 1646, 2555, 2662
Alw26I GTCTC 3 cut(s) 240, 1394, 1610
Alw44I GTGCAC 2 cut(s) 1642, 2551
AlwI GGATC 4 cut(s) 675, 2520, 2540, 2730
Ama87I CYCGRG 1 cut(s) 62
Aor13HI TCCGGA 1 cut(s) 379
AoxI GGCC 2 cut(s) 100, 2030
ApaLI GTGCAC 2 cut(s) 1642, 2551
ApoI RAATTY 2 cut(s) 17, 2505
Asp700I GAANNNNTTC 1 cut(s) 2109
AspLEI GCGC 5 cut(s) 56, 421, 551, 1165, 1424
AspS9I GGNCC 5 cut(s) 770, 1981, 2233, 2452, 2816
AsuNHI GCTAGC 1 cut(s) 1877
AvaI CYCGRG 1 cut(s) 62
AvaII GGWCC 5 cut(s) 770, 1981, 2233, 2452, 2816
AxyI CCTNAGG 1 cut(s) 735
BaeGI GKGCMC 2 cut(s) 1646, 2555
BanII GRGCYC 2 cut(s) 338, 2010
BauI CACGAG 1 cut(s) 1268
BbsI GAAGAC 2 cut(s) 606, 1509
Bbv12I GWGCWC 4 cut(s) 1605, 1646, 2555, 2662
BceAI ACGGC 6 cut(s) 148, 190, 631, 1180, 1419, 2017
BciT130I CCWGG 2 cut(s) 518, 1917
BciVI GTATCC 2 cut(s) 300, 2612
BclI TGATCA 1 cut(s) 1123
BcoDI GTCTC 3 cut(s) 240, 1394, 1610
BfaI CTAG 7 cut(s) 299, 324, 410, 1220, 1878, 1911, 2681
BfoI RGCGCY 1 cut(s) 422
BfrI CTTAAG 1 cut(s) 2018
BfuAI ACCTGC 1 cut(s) 942
BfuI GTATCC 2 cut(s) 300, 2612
BglII AGATCT 3 cut(s) 1491, 2251, 2672
BlpI GCTNAGC 1 cut(s) 2543
BmcAI AGTACT 1 cut(s) 408
Bme1390I CCNGG 2 cut(s) 518, 1917
Bme18I GGWCC 5 cut(s) 770, 1981, 2233, 2452, 2816
BmeT110I CYCGRG 1 cut(s) 62
BmgT120I GGNCC 5 cut(s) 770, 1981, 2233, 2452, 2816
BmiI GGNNCC 5 cut(s) 136, 815, 2234, 2235, 2818
BmrFI CCNGG 2 cut(s) 518, 1917
BmsI GCATC 3 cut(s) 999, 1075, 1213
BmtI GCTAGC 1 cut(s) 1881
BpiI GAAGAC 2 cut(s) 606, 1509
BplI GAGNNNNNCTC 6 cut(s) 77, 109, 245, 277, 2693, 2725
BpmI CTGGAG 2 cut(s) 79, 500
Bpu10I CCTNAGC 1 cut(s) 2009
Bpu1102I GCTNAGC 1 cut(s) 2543
BpuEI CTTGAG 5 cut(s) 200, 376, 1211, 1568, 1788
BsaBI GATNNNNATC 2 cut(s) 2064, 2721
BsaJI CCNNGG 7 cut(s) 63, 103, 817, 1507, 1611, 1647, 2626
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 669, 699, 1001, 1031, 1557, 1587
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 379, 653, 1922
Bse1I ACTGG 4 cut(s) 7, 544, 562, 2808
Bse21I CCTNAGG 1 cut(s) 735
Bse3DI GCAATG 5 cut(s) 688, 1164, 1888, 1935, 2409
Bse8I GATNNNNATC 2 cut(s) 2064, 2721
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 2 cut(s) 518, 1917
BseDI CCNNGG 7 cut(s) 63, 103, 817, 1507, 1611, 1647, 2626
BseGI GGATG 7 cut(s) 583, 1375, 1657, 1734, 2047, 2335, 2761
BseJI GATNNNNATC 2 cut(s) 2064, 2721
BseLI CCNNNNNNNGG 4 cut(s) 109, 379, 653, 1922
BseMI GCAATG 5 cut(s) 688, 1164, 1888, 1935, 2409
BseMII CTCAG 8 cut(s) 726, 1100, 1985, 2023, 2028, 2530, 2557, 2766
BseNI ACTGG 4 cut(s) 7, 544, 562, 2808
BseRI GAGGAG 3 cut(s) 47, 181, 1686
BseSI GKGCMC 2 cut(s) 1646, 2555
BseX3I CGGCCG 1 cut(s) 100
Bsh1236I CGCG 5 cut(s) 56, 505, 551, 711, 1422
Bsh1285I CGRYCG 2 cut(s) 103, 154
BshFI GGCC 2 cut(s) 102, 2032
BsiEI CGRYCG 2 cut(s) 103, 154
BsiHKAI GWGCWC 4 cut(s) 1605, 1646, 2555, 2662
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 314, 380
BslFI GGGAC 2 cut(s) 334, 2246
BslI CCNNNNNNNGG 4 cut(s) 109, 379, 653, 1922
BsmAI GTCTC 3 cut(s) 240, 1394, 1610
BsmBI CGTCTC 2 cut(s) 240, 1394
BsmFI GGGAC 2 cut(s) 334, 2246
BsmI GAATGC 1 cut(s) 2651
BsnI GGCC 2 cut(s) 102, 2032
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 6 cut(s) 338, 1605, 1646, 2010, 2555, 2662
Bsp13I TCCGGA 1 cut(s) 379
Bsp1407I TGTACA 1 cut(s) 2587
Bsp1720I GCTNAGC 1 cut(s) 2543
BspANI GGCC 2 cut(s) 102, 2032
BspCNI CTCAG 8 cut(s) 727, 1101, 1984, 2022, 2029, 2529, 2556, 2767
BspEI TCCGGA 1 cut(s) 379
BspFNI CGCG 5 cut(s) 56, 505, 551, 711, 1422
BspLI GGNNCC 5 cut(s) 136, 815, 2234, 2235, 2818
BspMI ACCTGC 1 cut(s) 942
BspOI GCTAGC 1 cut(s) 1881
BspPI GGATC 4 cut(s) 675, 2520, 2540, 2730
BspQI GCTCTTC 1 cut(s) 594
BspTI CTTAAG 1 cut(s) 2018
BsrDI GCAATG 5 cut(s) 688, 1164, 1888, 1935, 2409
BsrGI TGTACA 1 cut(s) 2587
BsrI ACTGG 4 cut(s) 7, 544, 562, 2808
BssECI CCNNGG 7 cut(s) 63, 103, 817, 1507, 1611, 1647, 2626
BssNAI GTATAC 1 cut(s) 1776
BssSI CACGAG 1 cut(s) 1268
BssT1I CCWWGG 1 cut(s) 1507
Bst1107I GTATAC 1 cut(s) 1776
Bst2BI CACGAG 1 cut(s) 1268
Bst2UI CCWGG 2 cut(s) 518, 1917
Bst4CI ACNGT 7 cut(s) 612, 692, 1051, 1573, 1985, 2367, 2456
Bst6I CTCTTC 4 cut(s) 206, 594, 1308, 2442
BstAFI CTTAAG 1 cut(s) 2018
BstAPI GCANNNNNTGC 1 cut(s) 468
BstAUI TGTACA 1 cut(s) 2587
BstC8I GCNNGC 5 cut(s) 1080, 1139, 1281, 1879, 2169
BstDSI CCRYGG 2 cut(s) 817, 1611
BstEII GGTNACC 2 cut(s) 857, 1184
BstF5I GGATG 7 cut(s) 583, 1375, 1657, 1734, 2047, 2335, 2761
BstFNI CGCG 5 cut(s) 56, 505, 551, 711, 1422
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 5 cut(s) 56, 421, 551, 1165, 1424
BstMAI GTCTC 3 cut(s) 240, 1394, 1610
BstMCI CGRYCG 2 cut(s) 103, 154
BstNI CCWGG 2 cut(s) 518, 1917
BstNSI RCATGY 3 cut(s) 1445, 2167, 2562
BstPI GGTNACC 2 cut(s) 857, 1184
BstSCI CCNGG 2 cut(s) 516, 1915
BstSLI GKGCMC 2 cut(s) 1646, 2555
BstUI CGCG 5 cut(s) 56, 505, 551, 711, 1422
BstV2I GAAGAC 2 cut(s) 606, 1509
BstX2I RGATCY 5 cut(s) 1491, 2251, 2512, 2532, 2672
BstXI CCANNNNNNTGG 2 cut(s) 1181, 2244
BstYI RGATCY 5 cut(s) 1491, 2251, 2512, 2532, 2672
BstZ17I GTATAC 1 cut(s) 1776
BstZI CGGCCG 1 cut(s) 100
Bsu36I CCTNAGG 1 cut(s) 735
BsuI GTATCC 2 cut(s) 300, 2612
BsuRI GGCC 2 cut(s) 102, 2032
BtgI CCRYGG 2 cut(s) 817, 1611
BtsCI GGATG 7 cut(s) 583, 1375, 1657, 1734, 2047, 2335, 2761
BtsIMutI CAGTG 5 cut(s) 14, 551, 555, 697, 2363
BveI ACCTGC 1 cut(s) 942
Cac8I GCNNGC 5 cut(s) 1080, 1139, 1281, 1879, 2169
CfoI GCGC 5 cut(s) 56, 421, 551, 1165, 1424
Cfr13I GGNCC 5 cut(s) 770, 1981, 2233, 2452, 2816
Csp6I GTAC 5 cut(s) 4, 407, 1483, 2562, 2588
CviQI GTAC 5 cut(s) 4, 407, 1483, 2562, 2588
DrdI GACNNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 137
EaeI YGGCCR 2 cut(s) 100, 2030
EagI CGGCCG 1 cut(s) 100
Eam1104I CTCTTC 4 cut(s) 206, 594, 1308, 2442
EarI CTCTTC 4 cut(s) 206, 594, 1308, 2442
EciI GGCGGA 1 cut(s) 28
EclXI CGGCCG 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 1507
Eco24I GRGCYC 2 cut(s) 338, 2010
Eco32I GATATC 2 cut(s) 824, 2335
Eco47I GGWCC 5 cut(s) 770, 1981, 2233, 2452, 2816
Eco52I CGGCCG 1 cut(s) 100
Eco57I CTGAAG 6 cut(s) 151, 191, 575, 905, 1122, 1730
Eco81I CCTNAGG 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 62
Eco91I GGTNACC 2 cut(s) 857, 1184
EcoO109I RGGNCCY 1 cut(s) 2233
EcoO65I GGTNACC 2 cut(s) 857, 1184
EcoRII CCWGG 2 cut(s) 516, 1915
EcoRV GATATC 2 cut(s) 824, 2335
EcoT14I CCWWGG 1 cut(s) 1507
EcoT38I GRGCYC 2 cut(s) 338, 2010
ErhI CCWWGG 1 cut(s) 1507
Esp3I CGTCTC 2 cut(s) 240, 1394
FaqI GGGAC 2 cut(s) 334, 2246
FauI CCCGC 2 cut(s) 37, 704
FbaI TGATCA 1 cut(s) 1123
FblI GTMKAC 3 cut(s) 730, 1775, 1907
FokI GGATG 7 cut(s) 590, 1382, 1664, 1721, 2034, 2342, 2768
FriOI GRGCYC 2 cut(s) 338, 2010
FspBI CTAG 7 cut(s) 299, 324, 410, 1220, 1878, 1911, 2681
GlaI GCGC 5 cut(s) 55, 420, 550, 1164, 1423
GsuI CTGGAG 2 cut(s) 79, 500
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 2 cut(s) 102, 2032
HapII CCGG 2 cut(s) 314, 380
HhaI GCGC 5 cut(s) 56, 421, 551, 1165, 1424
Hin6I GCGC 5 cut(s) 54, 419, 549, 1163, 1422
HinP1I GCGC 5 cut(s) 54, 419, 549, 1163, 1422
HincII GTYRAC 3 cut(s) 688, 731, 1339
HindII GTYRAC 3 cut(s) 688, 731, 1339
HindIII AAGCTT 1 cut(s) 2420
HpaI GTTAAC 1 cut(s) 688
HpaII CCGG 2 cut(s) 314, 380
Hpy166II GTNNAC 8 cut(s) 688, 731, 1339, 1644, 1776, 1908, 2273, 2553
Hpy188I TCNGA 6 cut(s) 426, 535, 572, 672, 1071, 2038
Hpy8I GTNNAC 8 cut(s) 688, 731, 1339, 1644, 1776, 1908, 2273, 2553
Hpy99I CGWCG 6 cut(s) 131, 134, 268, 334, 400, 2460
HpyCH4III ACNGT 7 cut(s) 612, 692, 1051, 1573, 1985, 2367, 2456
HpyCH4IV ACGT 2 cut(s) 395, 1350
HpySE526I ACGT 2 cut(s) 395, 1350
HspAI GCGC 5 cut(s) 54, 419, 549, 1163, 1422
KflI GGGWCCC 1 cut(s) 2233
Kpn2I TCCGGA 1 cut(s) 379
Ksp22I TGATCA 1 cut(s) 1123
KspAI GTTAAC 1 cut(s) 688
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 6 cut(s) 98, 140, 493, 519, 1919, 2665
LweI GCATC 3 cut(s) 999, 1075, 1213
MaeI CTAG 7 cut(s) 299, 324, 410, 1220, 1878, 1911, 2681
MaeII ACGT 2 cut(s) 395, 1350
MaeIII GTNAC 8 cut(s) 235, 604, 857, 950, 1184, 1567, 1701, 1869
MfeI CAATTG 1 cut(s) 1620
MflI RGATCY 5 cut(s) 1491, 2251, 2512, 2532, 2672
MhlI GDGCHC 6 cut(s) 338, 1605, 1646, 2010, 2555, 2662
MlyI GAGTC 2 cut(s) 1081, 1787
MroI TCCGGA 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 2109
MseI TTAA 9 cut(s) 474, 687, 1032, 1694, 1967, 2019, 2100, 2400, 2742
MslI CAYNNNNRTG 2 cut(s) 1440, 2178
MspA1I CMGCKG 1 cut(s) 99
MspCI CTTAAG 1 cut(s) 2018
MspI CCGG 2 cut(s) 314, 380
MspR9I CCNGG 2 cut(s) 518, 1917
MunI CAATTG 1 cut(s) 1620
Mva1269I GAATGC 1 cut(s) 2651
MvaI CCWGG 2 cut(s) 518, 1917
MvnI CGCG 5 cut(s) 56, 505, 551, 711, 1422
NheI GCTAGC 1 cut(s) 1877
NlaIV GGNNCC 5 cut(s) 136, 815, 2234, 2235, 2818
NmeAIII GCCGAG 1 cut(s) 128
NmuCI GTSAC 4 cut(s) 235, 604, 1184, 1869
NspI RCATGY 3 cut(s) 1445, 2167, 2562
PaqCI CACCTGC 1 cut(s) 942
PciSI GCTCTTC 1 cut(s) 594
PcsI WCGNNNNNNNCGW 4 cut(s) 27, 126, 135, 272
PctI GAATGC 1 cut(s) 2651
PdmI GAANNNNTTC 1 cut(s) 2109
PfeI GAWTC 9 cut(s) 58, 567, 640, 1039, 1233, 1591, 1658, 2216, 2432
PflFI GACNNNGTC 1 cut(s) 610
PleI GAGTC 2 cut(s) 1080, 1787
PpsI GAGTC 2 cut(s) 1080, 1787
PpuMI RGGWCCY 1 cut(s) 2233
PsiI TTATAA 1 cut(s) 965
Psp5II RGGWCCY 1 cut(s) 2233
Psp6I CCWGG 2 cut(s) 516, 1915
PspEI GGTNACC 2 cut(s) 857, 1184
PspGI CCWGG 2 cut(s) 516, 1915
PspN4I GGNNCC 5 cut(s) 136, 815, 2234, 2235, 2818
PspPI GGNCC 5 cut(s) 770, 1981, 2233, 2452, 2816
PspPPI RGGWCCY 1 cut(s) 2233
PsuI RGATCY 5 cut(s) 1491, 2251, 2512, 2532, 2672
PsyI GACNNNGTC 1 cut(s) 610
RsaI GTAC 5 cut(s) 5, 408, 1484, 2563, 2589
RsaNI GTAC 5 cut(s) 4, 407, 1483, 2562, 2588
RseI CAYNNNNRTG 2 cut(s) 1440, 2178
SalI GTCGAC 1 cut(s) 729
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 9 cut(s) 474, 687, 1032, 1694, 1967, 2019, 2100, 2400, 2742
Sau96I GGNCC 5 cut(s) 770, 1981, 2233, 2452, 2816
ScaI AGTACT 1 cut(s) 408
SchI GAGTC 2 cut(s) 1081, 1787
ScrFI CCNGG 2 cut(s) 518, 1917
SduI GDGCHC 6 cut(s) 338, 1605, 1646, 2010, 2555, 2662
SfaNI GCATC 3 cut(s) 999, 1075, 1213
SinI GGWCC 5 cut(s) 770, 1981, 2233, 2452, 2816
SmiMI CAYNNNNRTG 2 cut(s) 1440, 2178
SmlI CTYRAG 6 cut(s) 215, 355, 1190, 1547, 1803, 2018
SmoI CTYRAG 6 cut(s) 215, 355, 1190, 1547, 1803, 2018
SspI AATATT 1 cut(s) 2264
SspMI CTAG 7 cut(s) 299, 324, 410, 1220, 1878, 1911, 2681
StyD4I CCNGG 2 cut(s) 516, 1915
StyI CCWWGG 1 cut(s) 1507
TaaI ACNGT 7 cut(s) 612, 692, 1051, 1573, 1985, 2367, 2456
TaiI ACGT 2 cut(s) 398, 1353
TatI WGTACW 3 cut(s) 406, 2561, 2587
TauI GCSGC 3 cut(s) 78, 102, 1422
TfiI GAWTC 9 cut(s) 58, 567, 640, 1039, 1233, 1591, 1658, 2216, 2432
Tru1I TTAA 9 cut(s) 474, 687, 1032, 1694, 1967, 2019, 2100, 2400, 2742
Tru9I TTAA 9 cut(s) 474, 687, 1032, 1694, 1967, 2019, 2100, 2400, 2742
TscAI CASTG 5 cut(s) 14, 551, 562, 697, 2370
TseFI GTSAC 4 cut(s) 235, 604, 1184, 1869
Tsp45I GTSAC 4 cut(s) 235, 604, 1184, 1869
TspDTI ATGAA 9 cut(s) 619, 768, 1345, 1452, 1723, 2105, 2147, 2184, 2196
TspGWI ACGGA 3 cut(s) 127, 184, 806
TspRI CASTG 5 cut(s) 14, 551, 562, 697, 2370
Tth111I GACNNNGTC 1 cut(s) 610
Vha464I CTTAAG 1 cut(s) 2018
VneI GTGCAC 2 cut(s) 1642, 2551
VpaK11BI GGWCC 5 cut(s) 770, 1981, 2233, 2452, 2816
XapI RAATTY 2 cut(s) 17, 2505
XbaI TCTAGA 3 cut(s) 298, 1219, 2680
XceI RCATGY 3 cut(s) 1445, 2167, 2562
XmiI GTMKAC 3 cut(s) 730, 1775, 1907
XmnI GAANNNNTTC 1 cut(s) 2109
XspI CTAG 7 cut(s) 299, 324, 410, 1220, 1878, 1911, 2681
ZrmI AGTACT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.