MD17G1113900.v1.1

Vacuolar protein-sorting-associated protein 11 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012419
Physical Location & Seq
Reverse (-)
9766361 .. 9770750
4390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1113900.v1.1.491

Sequence Viewer

Length: 2862 bp
ATGTACCAGTGGAGGAAATTCGAGTTCTTCGAGGAGAAATTGGCGGGAAAGTGCGCGATTCCCGAGGAAGTGGAAGGGAAGATCGAGTGTTGCTCGAGCGGCAGAGGGAAGGTGGTGATCGGCTGCGATGACGGCACAGTCAGCTTCCTCGACCGCGGCCTCAACTACAGCTACGGATTCCAAGCTCACTCCTCCTCCGCGCTGTTCCTTCAGCAGCTCAAGCAACGCAACTATTTGGTGACAATTGGAGAAGATGAACAAATTACTCCGCAGCAATCAGCAATGTGCCTCAAGGTTTTCGACCTGGATAGGATGCAGTCCGAGGGCTCGAGCTCAAGCACAACGAGCCCTGATTGCATTGGAATCTTGAGGATATTCACTAACCAATTTCCTGAAGCAAAGATTACATCGTTTTTAGTACTAGAGGAAGCACCTCCGATATTGCTTATAGCTATTGGATTAGATAATGGCTCAATTTACTGCATTAAAGGGGATATTGCACGAGAGCGAATCACCCGGTTCAAGCTTCAGGTGGAAAACCTTTCTGACAAGAGCCAGTCCTCGGTTACAGGACTGGGGTTCAGAGTGGACGGTCAAGCGCTTCAGTTGTTTGCTGTGACTCCGTCTTCTGTGAGCTTGTTCATCTTGCAGAATCAACCCTCAAACACAAGGAGGCAGACCCTAGATCAGATTGGAAGTAATGTGAACAGTGTTGCGATGAGTGATCGCTTGGAGTTGATAATTGGTCGACCTGAAGCAGTTTACTTTTATGAAGTTGATGGGCGTGGACCATGTTGGGCTTTTGAAGGAGAAAAGAAATTTTTAGGGTGGTTTCGTGGATACCTTCTTTGTGTTATTGCAGATCAAAGAAATAGCAGCAACACTTTCAACATTTATGACCTGAAGAACCGTTTAATTGCTCACAGCCTAGTGGTTAAAGAGGTTTCTCACATGCTTTGTGAATGGGGTAACATAATACTTATCATGGCTGACAAGTCAGCTTTGTGCATTGGGGAGAAGGATATGGAGAGCAAGTTAGATATGCTCTTTAAGAAGAATCTTTACACTGTTGCCATCAACCTTGTTCAAAGTCAGCAAGCAGATGCTGCCGCAACTGCAGAAGTGCTGAGGAAGTATGGGGATCATCTATATAGCAAGCAAGATTATGATGAGGCAATGGCCCAGTATATCCATACCATTGGTCACCTTGAGCCTTCTTATGTGATACAGAAGTTTCTAGATGCACAACGAATCTACAACCTGACAAATTACTTGGAAAAATTGCATGAGAAGGGGCTTGCTTCCAAAGATCATACCACTCTTCTACTGAATTGCTACACCAAATTGAAAGATGTTGACAAGCTAAATGTGTTCATTAAAAGTGAGGATGGTATTGGGGAACACAAATTTGATGTGGAGACTGCAATAAGAGTCTGCCGTGCTACCAATTACCATGAACATGCAATGTATGTTGCTAAAAAGGCTGGGAAGCACGAATGGTATTTAAAGATCTTACTTGAAGATCTTGGCCGATATGAGGAAGCCTTGCAATATATTTCAAGCCTTGAACCAAGCCAAGCTGGAGCGACAGTGGAAGAGTATGGTAAGATTCTTATAGAGCACAAGCCTGTGGAAACAATTGAGATACTCATGAGGCTGTGCACTGAGGATGGCGAATCATCCAAGAGAGGAGCTGCTAATGGTGCGTACTTGACAATGCTGCCATCTCCAGTTGATTTTCTCAACATCTTCATCCATCATCTTCCCTGGCTTATGGATTTTCTTGAAAAATATACCAACAAGGTCAAGGACTCTCCTGCTCAAGTAGAAATCCATAACACACTCTTGGAGTTGTACTTGTCCAATGACTTGAGCTTCACATCATTATCACAAGCTAGCAATGGCGAAGATCTGAATCTCAGGGCTAGATCAGGTGCAGCTGCAACATCAAGAAGCCAGTCCAATGGGAAATTGTTTGCTGATACCAAAGATTCAAACAAGGAAAAGGACCGTTTGGAAAAGCAAGAGAAAGGGGTACAACTTCTTAAGAGCGCATGGCCATCTGACCAGGAACTTCCGCTTTATGATGTTGACCTTGCCATAATTCTTTGTGAAATGAATGCATTTAAGGAAGGTCTTCTATATCTCTATGAAAAATTGAAACTCTATAAAGAGGTGATTGCTTGCTACATGCAAGCTCATGACCATGAGGGTTTGATTGCATGCTGCAAACGACTAGGGGATTCAGGTAAGGGCGGGGACCCAACTCTTTGGGCAGATCTGCTAAAATATTTTGGTGAACTTGGAGAAGATTGCTCCAAAGAAGTGAAGGAAGTTTTGACATATATAGAAAGGGATGACATACTGCCTCCTATCATTGTTCTTCAAACACTATCCAGAAATCCATGTCTCACACTCTCTGTCATTAAGGATTACATTGCTAGAAAGCTTGAACAAGAATCAAAATTGATAGAAGAGGACCGTCGAGCAATTGAGAAGTATCAGGAAACCACATCGGCAATGAGAAACGAAATTCAGGATCTTAGGACAAATGCCAGAATCTTTCAGCTCAGCAAGTGCACTGCATGCACTTTCACCCTCGATCTCCCAGCTGTACATTTTATGTGTATCCACTCGTTCCATCAGCGTTGCCTCGGCGATAATGAGAAGGAATGTCCTGTGTGTGCTCCTGAATACAAATCTGTTCTCGAAACAAAAAGAAGTCTAGAGCAGAACTCAAAAGATCAGGATCGGTTCTTCCAGCAAGTTAAAAGTTCAAAGGACGGTTTTTCTGTGATCGCCGAGTATTTTGGGAAGGGGGTGATCAGCAAAACCACTAATGGAACCACAGGTGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000323 GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005768 GO:0005769 GO:0005770 GO:0005773 GO:0005774 GO:0005856 GO:0005884 GO:0006810 GO:0006887 GO:0006904 GO:0006906 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0008150 GO:0008333 GO:0009705 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010256 GO:0010604 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019222 GO:0019904 GO:0019905 GO:0022406 GO:0030139 GO:0030674 GO:0030897 GO:0031090 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031338 GO:0031410 GO:0031982 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032879 GO:0032940 GO:0032991 GO:0033043 GO:0033263 GO:0034058 GO:0035542 GO:0042144 GO:0042176 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046903 GO:0046907 GO:0048278 GO:0048284 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051049 GO:0051050 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051640 GO:0051641 GO:0051649 GO:0060090 GO:0060255 GO:0060341 GO:0060627 GO:0061024 GO:0061025 GO:0065007 GO:0071840 GO:0080090 GO:0080171 GO:0090174 GO:0097576 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140029 GO:0140056 GO:1901998 GO:1902115 GO:1903362 GO:1903364 GO:1903649 GO:1903651 GO:2000641 GO:2000643
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin VPS11_C zf-C3H2C3

Protein Analysis

954

Amino Acids

107.75

Weight (kDa)

5.57

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEP5_VPS11_N PF23341 4 - 339 1.7e-93 PEP5/VPS11 N-terminal
VPS11_N PF23266 25 - 334 1.6e-120 VPS11-like, N-terminal beta-propeller
TPR_PEP5_VPS11 PF23356 384 - 625 3.8e-62 PEP5/VPS11 Clathrin repeat
Clathrin PF00637 404 - 544 7.2e-07 Region in Clathrin and VPS
TPR_PEP5_VPS11 PF23356 664 - 796 2.8e-18 PEP5/VPS11 Clathrin repeat
zf-C3H2C3 PF17122 860 - 895 6.8e-06 Zinc-finger
VPS11_C PF12451 900 - 942 3.9e-14 Vacuolar protein sorting protein 11 C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012419)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05170
fragaria_vesca FvH4_6g40910 FvH4_6g40910
malus_domestica MD09G1123500.v1.1 MD17G1113900.v1.1
prunus_persica Prupe.3G200600_v2.0.a1
pyrus_communis pycom09g04750 pycom17g10560
rosa_chinensis RchiOBHm_Chr2g0156491
rosa_laevigata RLG00000020917
rosa_multiflora Rmu_co8501807.1_g000001 Rmu_sc0005392.1_g000009
rosa_roxburghii Rroxscaffold_2G00092980
rosa_rugosa Rorug02G0453700
rosa_samantha Rh2AG519800 Rh2BG532100 Rh2CG504800 Rh2DG541100
rosa_wichuraiana Rw2G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 137
AccBSI CCGCTC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 748
AccII CGCG 3 cut(s) 56, 156, 200
AciI CCGC 9 cut(s) 44, 99, 154, 156, 198, 269, 1110, 2078, 2256
AclWI GGATC 3 cut(s) 1149, 2547, 2757
AcoI YGGCCR 2 cut(s) 1528, 2057
AcsI RAATTY 4 cut(s) 17, 818, 1406, 2532
AcuI CTGAAG 6 cut(s) 194, 414, 512, 587, 774, 923
AfaI GTAC 6 cut(s) 5, 420, 1709, 1856, 2037, 2616
AfeI AGCGCT 1 cut(s) 600
AfiI CCNNNNNNNGG 2 cut(s) 562, 1537
AflII CTTAAG 1 cut(s) 2045
AjnI CCWGG 3 cut(s) 303, 1766, 2067
Alw21I GWGCWC 5 cut(s) 335, 1623, 1664, 2582, 2689
Alw26I GTCTC 2 cut(s) 1412, 2414
Alw44I GTGCAC 2 cut(s) 1660, 2578
AlwI GGATC 3 cut(s) 1149, 2547, 2757
AlwNI CAGNNNCTG 1 cut(s) 1106
Ama87I CYCGRG 3 cut(s) 62, 94, 328
Aor51HI AGCGCT 1 cut(s) 600
AoxI GGCC 4 cut(s) 157, 1179, 1528, 2057
ApaLI GTGCAC 2 cut(s) 1660, 2578
ApoI RAATTY 4 cut(s) 17, 818, 1406, 2532
Asp700I GAANNNNTTC 1 cut(s) 2136
AspLEI GCGC 4 cut(s) 56, 202, 601, 2054
AspS9I GGNCC 5 cut(s) 788, 1180, 2008, 2260, 2479
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 8 cut(s) 127, 250, 505, 1196, 2188, 2309, 2587, 2833
AsuNHI GCTAGC 1 cut(s) 1895
AvaI CYCGRG 3 cut(s) 62, 94, 328
AvaII GGWCC 4 cut(s) 788, 2008, 2260, 2479
BaeGI GKGCMC 2 cut(s) 1664, 2582
BaeI ACNNNNGTAYC 2 cut(s) 1217, 1250
BalI TGGCCA 1 cut(s) 2059
BanII GRGCYC 3 cut(s) 329, 335, 350
BarI GAAGNNNNNNTAC 2 cut(s) 1046, 1078
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 2 cut(s) 618, 2129
Bbv12I GWGCWC 5 cut(s) 335, 1623, 1664, 2582, 2689
BbvCI CCTCAGC 1 cut(s) 1127
BccI CCATC 8 cut(s) 773, 1082, 1382, 1664, 1732, 1764, 2068, 2649
BceAI ACGGC 2 cut(s) 148, 1422
BciT130I CCWGG 3 cut(s) 305, 1768, 2069
BciVI GTATCC 2 cut(s) 833, 2639
BclI TGATCA 1 cut(s) 2823
BcnI CCSGG 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 1412, 2414
BfaI CTAG 9 cut(s) 422, 683, 929, 1238, 1896, 1926, 2237, 2442, 2726
BfmI CTRYAG 2 cut(s) 166, 1116
BfoI RGCGCY 1 cut(s) 602
BfrI CTTAAG 1 cut(s) 2045
BfuI GTATCC 2 cut(s) 833, 2639
BglII AGATCT 4 cut(s) 1509, 1522, 1909, 2278
BlpI GCTNAGC 1 cut(s) 2570
BmcAI AGTACT 1 cut(s) 420
Bme1390I CCNGG 4 cut(s) 305, 517, 1768, 2069
Bme18I GGWCC 4 cut(s) 788, 2008, 2260, 2479
BmeT110I CYCGRG 3 cut(s) 62, 94, 328
BmgT120I GGNCC 5 cut(s) 788, 1180, 2008, 2260, 2479
BmiI GGNNCC 3 cut(s) 2261, 2262, 2845
BmrFI CCNGG 4 cut(s) 305, 517, 1768, 2069
BmrI ACTGGG 2 cut(s) 584, 1177
BmsI GCATC 3 cut(s) 303, 1093, 1231
BmtI GCTAGC 1 cut(s) 1899
BmuI ACTGGG 2 cut(s) 584, 1177
BpiI GAAGAC 2 cut(s) 618, 2129
BplI GAGNNNNNCTC 4 cut(s) 77, 109, 2720, 2752
BpmI CTGGAG 2 cut(s) 1602, 1713
Bpu10I CCTNAGC 1 cut(s) 1127
Bpu1102I GCTNAGC 1 cut(s) 2570
BpuEI CTTGAG 7 cut(s) 203, 275, 319, 388, 1229, 1806, 1891
BpuMI CCSGG 1 cut(s) 517
BsaBI GATNNNNATC 2 cut(s) 1914, 2748
BsaJI CCNNGG 6 cut(s) 63, 154, 321, 561, 1766, 2653
BsaXI ACNNNNNCTCC 6 cut(s) 179, 209, 1019, 1049, 1575, 1605
Bsc4I CCNNNNNNNGG 2 cut(s) 562, 1537
Bse1I ACTGG 6 cut(s) 7, 556, 579, 1183, 1730, 1957
Bse3DI GCAATG 6 cut(s) 288, 1182, 1470, 1906, 2436, 2526
Bse8I GATNNNNATC 2 cut(s) 1914, 2748
BseBI CCWGG 3 cut(s) 305, 1768, 2069
BseDI CCNNGG 6 cut(s) 63, 154, 321, 561, 1766, 2653
BseGI GGATG 6 cut(s) 318, 1393, 1675, 1679, 1752, 2362
BseJI GATNNNNATC 2 cut(s) 1914, 2748
BseLI CCNNNNNNNGG 2 cut(s) 562, 1537
BseMI GCAATG 6 cut(s) 288, 1182, 1470, 1906, 2436, 2526
BseMII CTCAG 4 cut(s) 1118, 1656, 1933, 2584
BseNI ACTGG 6 cut(s) 7, 556, 579, 1183, 1730, 1957
BseRI GAGGAG 4 cut(s) 47, 181, 184, 1704
BseSI GKGCMC 2 cut(s) 1664, 2582
BseYI CCCAGC 2 cut(s) 1484, 2608
BsgI GTGCAG 1 cut(s) 1956
Bsh1236I CGCG 3 cut(s) 56, 156, 200
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 4 cut(s) 159, 1181, 1530, 2059
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 5 cut(s) 335, 1623, 1664, 2582, 2689
BsiHKCI CYCGRG 3 cut(s) 62, 94, 328
BsiSI CCGG 1 cut(s) 517
BslFI GGGAC 1 cut(s) 2273
BslI CCNNNNNNNGG 2 cut(s) 562, 1537
BsmAI GTCTC 2 cut(s) 1412, 2414
BsmFI GGGAC 1 cut(s) 2273
BsmI GAATGC 1 cut(s) 2125
BsnI GGCC 4 cut(s) 159, 1181, 1530, 2059
BsoBI CYCGRG 3 cut(s) 62, 94, 328
Bsp1286I GDGCHC 7 cut(s) 329, 335, 350, 1623, 1664, 2582, 2689
Bsp1407I TGTACA 1 cut(s) 2614
Bsp1720I GCTNAGC 1 cut(s) 2570
BspACI CCGC 9 cut(s) 44, 99, 154, 156, 198, 269, 1110, 2078, 2256
BspANI GGCC 4 cut(s) 159, 1181, 1530, 2059
BspCNI CTCAG 4 cut(s) 1119, 1657, 1932, 2583
BspFNI CGCG 3 cut(s) 56, 156, 200
BspHI TCATGA 2 cut(s) 1650, 2200
BspLI GGNNCC 3 cut(s) 2261, 2262, 2845
BspMAI CTGCAG 1 cut(s) 1120
BspOI GCTAGC 1 cut(s) 1899
BspPI GGATC 3 cut(s) 1149, 2547, 2757
BspTI CTTAAG 1 cut(s) 2045
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 6 cut(s) 288, 1182, 1470, 1906, 2436, 2526
BsrGI TGTACA 1 cut(s) 2614
BsrI ACTGG 6 cut(s) 7, 556, 579, 1183, 1730, 1957
BssECI CCNNGG 6 cut(s) 63, 154, 321, 561, 1766, 2653
BssSI CACGAG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 3 cut(s) 305, 1768, 2069
Bst4CI ACNGT 9 cut(s) 139, 593, 710, 911, 1069, 1591, 2012, 2483, 2786
Bst6I CTCTTC 3 cut(s) 1326, 1590, 2469
BstAFI CTTAAG 1 cut(s) 2045
BstAPI GCANNNNNTGC 2 cut(s) 1106, 2586
BstAUI TGTACA 1 cut(s) 2614
BstC8I GCNNGC 8 cut(s) 1098, 1157, 1299, 1897, 2185, 2196, 2224, 2587
BstDEI CTNAG 5 cut(s) 1127, 1665, 1919, 2543, 2570
BstDSI CCRYGG 1 cut(s) 154
BstEII GGTNACC 1 cut(s) 1202
BstF5I GGATG 6 cut(s) 318, 1393, 1675, 1679, 1752, 2362
BstFNI CGCG 3 cut(s) 56, 156, 200
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 4 cut(s) 56, 202, 601, 2054
BstMAI GTCTC 2 cut(s) 1412, 2414
BstMCI CGRYCG 1 cut(s) 154
BstNI CCWGG 3 cut(s) 305, 1768, 2069
BstNSI RCATGY 5 cut(s) 955, 1463, 2194, 2226, 2589
BstPI GGTNACC 1 cut(s) 1202
BstSCI CCNGG 4 cut(s) 303, 515, 1766, 2067
BstSFI CTRYAG 2 cut(s) 166, 1116
BstSLI GKGCMC 2 cut(s) 1664, 2582
BstUI CGCG 3 cut(s) 56, 156, 200
BstV2I GAAGAC 2 cut(s) 618, 2129
BstX2I RGATCY 5 cut(s) 1509, 1522, 1909, 2278, 2539
BstXI CCANNNNNNTGG 3 cut(s) 1199, 1964, 2271
BstYI RGATCY 5 cut(s) 1509, 1522, 1909, 2278, 2539
BsuI GTATCC 2 cut(s) 833, 2639
BsuRI GGCC 4 cut(s) 159, 1181, 1530, 2059
BtgI CCRYGG 1 cut(s) 154
BtgZI GCGATG 2 cut(s) 141, 731
BtsCI GGATG 6 cut(s) 318, 1393, 1675, 1679, 1752, 2362
BtsI GCAGTG 1 cut(s) 2580
BtsIMutI CAGTG 6 cut(s) 14, 715, 1065, 1596, 1662, 2580
Cac8I GCNNGC 8 cut(s) 1098, 1157, 1299, 1897, 2185, 2196, 2224, 2587
CaiI CAGNNNCTG 1 cut(s) 1106
CciI TCATGA 2 cut(s) 1650, 2200
CfoI GCGC 4 cut(s) 56, 202, 601, 2054
Cfr13I GGNCC 5 cut(s) 788, 1180, 2008, 2260, 2479
Cfr42I CCGCGG 1 cut(s) 157
Csp6I GTAC 6 cut(s) 4, 419, 1708, 1855, 2036, 2615
CviQI GTAC 6 cut(s) 4, 419, 1708, 1855, 2036, 2615
DdeI CTNAG 5 cut(s) 1127, 1665, 1919, 2543, 2570
DraI TTTAAA 1 cut(s) 1506
DrdI GACNNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 137
EaeI YGGCCR 2 cut(s) 1528, 2057
Eam1104I CTCTTC 3 cut(s) 1326, 1590, 2469
EarI CTCTTC 3 cut(s) 1326, 1590, 2469
Ecl136II GAGCTC 1 cut(s) 333
Eco24I GRGCYC 3 cut(s) 329, 335, 350
Eco47I GGWCC 4 cut(s) 788, 2008, 2260, 2479
Eco47III AGCGCT 1 cut(s) 600
Eco53kI GAGCTC 1 cut(s) 333
Eco57I CTGAAG 6 cut(s) 194, 414, 512, 587, 774, 923
Eco88I CYCGRG 3 cut(s) 62, 94, 328
Eco91I GGTNACC 1 cut(s) 1202
EcoICRI GAGCTC 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 2260
EcoO65I GGTNACC 1 cut(s) 1202
EcoRII CCWGG 3 cut(s) 303, 1766, 2067
EcoT22I ATGCAT 1 cut(s) 2125
EcoT38I GRGCYC 3 cut(s) 329, 335, 350
FaqI GGGAC 1 cut(s) 2273
FauI CCCGC 2 cut(s) 37, 2249
FbaI TGATCA 1 cut(s) 2823
FblI GTMKAC 1 cut(s) 748
FokI GGATG 6 cut(s) 325, 1400, 1666, 1682, 1739, 2369
FriOI GRGCYC 3 cut(s) 329, 335, 350
FspBI CTAG 9 cut(s) 422, 683, 929, 1238, 1896, 1926, 2237, 2442, 2726
GlaI GCGC 4 cut(s) 55, 201, 600, 2053
GsaI CCCAGC 2 cut(s) 1488, 2612
GsuI CTGGAG 2 cut(s) 1602, 1713
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 4 cut(s) 159, 1181, 1530, 2059
HapII CCGG 1 cut(s) 517
HhaI GCGC 4 cut(s) 56, 202, 601, 2054
Hin6I GCGC 4 cut(s) 54, 200, 599, 2052
HinP1I GCGC 4 cut(s) 54, 200, 599, 2052
HincII GTYRAC 3 cut(s) 749, 1357, 2092
HindII GTYRAC 3 cut(s) 749, 1357, 2092
HindIII AAGCTT 2 cut(s) 524, 2447
HpaII CCGG 1 cut(s) 517
HphI GGTGA 8 cut(s) 127, 250, 505, 1196, 2188, 2309, 2587, 2833
Hpy188I TCNGA 7 cut(s) 322, 438, 547, 584, 690, 1914, 2065
Hpy99I CGWCG 1 cut(s) 2487
HpyCH4III ACNGT 9 cut(s) 139, 593, 710, 911, 1069, 1591, 2012, 2483, 2786
HpyF3I CTNAG 5 cut(s) 1127, 1665, 1919, 2543, 2570
HspAI GCGC 4 cut(s) 54, 200, 599, 2052
KflI GGGWCCC 1 cut(s) 2260
Ksp22I TGATCA 1 cut(s) 2823
KspI CCGCGG 1 cut(s) 157
LmnI GCTCC 4 cut(s) 1583, 1691, 2321, 2692
LweI GCATC 3 cut(s) 303, 1093, 1231
MaeI CTAG 9 cut(s) 422, 683, 929, 1238, 1896, 1926, 2237, 2442, 2726
MaeIII GTNAC 5 cut(s) 238, 565, 616, 968, 1202
MbiI CCGCTC 1 cut(s) 99
MfeI CAATTG 3 cut(s) 243, 1638, 2490
MflI RGATCY 5 cut(s) 1509, 1522, 1909, 2278, 2539
MhlI GDGCHC 7 cut(s) 329, 335, 350, 1623, 1664, 2582, 2689
MlsI TGGCCA 1 cut(s) 2059
MluNI TGGCCA 1 cut(s) 2059
MlyI GAGTC 3 cut(s) 613, 1440, 1805
Mox20I TGGCCA 1 cut(s) 2059
Mph1103I ATGCAT 1 cut(s) 2125
MroXI GAANNNNTTC 1 cut(s) 2136
MscI TGGCCA 1 cut(s) 2059
MslI CAYNNNNRTG 2 cut(s) 1458, 2205
Msp20I TGGCCA 1 cut(s) 2059
MspA1I CMGCKG 3 cut(s) 156, 1940, 2612
MspCI CTTAAG 1 cut(s) 2045
MspI CCGG 1 cut(s) 517
MspR9I CCNGG 4 cut(s) 305, 517, 1768, 2069
MunI CAATTG 3 cut(s) 243, 1638, 2490
Mva1269I GAATGC 1 cut(s) 2125
MvaI CCWGG 3 cut(s) 305, 1768, 2069
MvnI CGCG 3 cut(s) 56, 156, 200
NciI CCSGG 1 cut(s) 517
NheI GCTAGC 1 cut(s) 1895
NlaIV GGNNCC 3 cut(s) 2261, 2262, 2845
NmeAIII GCCGAG 2 cut(s) 2634, 2827
NmuCI GTSAC 3 cut(s) 238, 616, 1202
NsiI ATGCAT 1 cut(s) 2125
NspI RCATGY 5 cut(s) 955, 1463, 2194, 2226, 2589
PaeI GCATGC 2 cut(s) 2226, 2589
PaeR7I CTCGAG 2 cut(s) 94, 328
PagI TCATGA 2 cut(s) 1650, 2200
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 1 cut(s) 2125
PdmI GAANNNNTTC 1 cut(s) 2136
PflFI GACNNNGTC 1 cut(s) 622
PleI GAGTC 3 cut(s) 613, 1439, 1805
PpsI GAGTC 3 cut(s) 613, 1439, 1805
PpuMI RGGWCCY 1 cut(s) 2260
Psp124BI GAGCTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 2260
Psp6I CCWGG 3 cut(s) 303, 1766, 2067
PspEI GGTNACC 1 cut(s) 1202
PspFI CCCAGC 2 cut(s) 1484, 2608
PspGI CCWGG 3 cut(s) 303, 1766, 2067
PspN4I GGNNCC 3 cut(s) 2261, 2262, 2845
PspPI GGNCC 5 cut(s) 788, 1180, 2008, 2260, 2479
PspPPI RGGWCCY 1 cut(s) 2260
PspXI VCTCGAGB 2 cut(s) 94, 328
PstI CTGCAG 1 cut(s) 1120
PstNI CAGNNNCTG 1 cut(s) 1106
PsuI RGATCY 5 cut(s) 1509, 1522, 1909, 2278, 2539
PsyI GACNNNGTC 1 cut(s) 622
PvuII CAGCTG 2 cut(s) 1940, 2612
RsaI GTAC 6 cut(s) 5, 420, 1709, 1856, 2037, 2616
RsaNI GTAC 6 cut(s) 4, 419, 1708, 1855, 2036, 2615
RseI CAYNNNNRTG 2 cut(s) 1458, 2205
SacI GAGCTC 1 cut(s) 335
SacII CCGCGG 1 cut(s) 157
SalI GTCGAC 1 cut(s) 747
Sau96I GGNCC 5 cut(s) 788, 1180, 2008, 2260, 2479
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 3 cut(s) 613, 1440, 1805
ScrFI CCNGG 4 cut(s) 305, 517, 1768, 2069
SduI GDGCHC 7 cut(s) 329, 335, 350, 1623, 1664, 2582, 2689
SfaNI GCATC 3 cut(s) 303, 1093, 1231
SfcI CTRYAG 2 cut(s) 166, 1116
Sfr274I CTCGAG 2 cut(s) 94, 328
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 4 cut(s) 788, 2008, 2260, 2479
SlaI CTCGAG 2 cut(s) 94, 328
SmiMI CAYNNNNRTG 2 cut(s) 1458, 2205
SphI GCATGC 2 cut(s) 2226, 2589
SsiI CCGC 9 cut(s) 44, 99, 154, 156, 198, 269, 1110, 2078, 2256
SspI AATATT 1 cut(s) 2291
SspMI CTAG 9 cut(s) 422, 683, 929, 1238, 1896, 1926, 2237, 2442, 2726
SstI GAGCTC 1 cut(s) 335
StyD4I CCNGG 4 cut(s) 303, 515, 1766, 2067
TaaI ACNGT 9 cut(s) 139, 593, 710, 911, 1069, 1591, 2012, 2483, 2786
TatI WGTACW 3 cut(s) 418, 1854, 2614
TauI GCSGC 3 cut(s) 102, 159, 1112
TscAI CASTG 6 cut(s) 14, 715, 1072, 1596, 1669, 2587
TseFI GTSAC 3 cut(s) 238, 616, 1202
Tsp45I GTSAC 3 cut(s) 238, 616, 1202
TspDTI ATGAA 8 cut(s) 270, 631, 786, 1363, 1470, 1741, 2132, 2166
TspGWI ACGGA 2 cut(s) 189, 612
TspRI CASTG 6 cut(s) 14, 715, 1072, 1596, 1669, 2587
Tth111I GACNNNGTC 1 cut(s) 622
Vha464I CTTAAG 1 cut(s) 2045
VneI GTGCAC 2 cut(s) 1660, 2578
VpaK11BI GGWCC 4 cut(s) 788, 2008, 2260, 2479
XapI RAATTY 4 cut(s) 17, 818, 1406, 2532
XbaI TCTAGA 2 cut(s) 1237, 2725
XceI RCATGY 5 cut(s) 955, 1463, 2194, 2226, 2589
XhoI CTCGAG 2 cut(s) 94, 328
XmiI GTMKAC 1 cut(s) 748
XmnI GAANNNNTTC 1 cut(s) 2136
XspI CTAG 9 cut(s) 422, 683, 929, 1238, 1896, 1926, 2237, 2442, 2726
ZrmI AGTACT 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 2125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.