Rroxscaffold_2G00092980

Vacuolar protein-sorting-associated protein 11 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012419
Physical Location & Seq
Reverse (-)
14412334 .. 14417086
4753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092980.1

Sequence Viewer

Length: 2871 bp
ATGTACCAGTGGAGGAAATTCGAGTTCTTCGAGGAGAAATTGGCGGGAAAGTGCGCGATTCCCGAGGAAGTAACGGGCAAGATCGAGTGCTGTTCGAGCGGGCGAGGCAAGGTGGTGATCGGCTGCGACGACGGCACCGTCAGCTTCCTCGACCGCAGCCTCCACTTCAGCTACGGCTTCCAAGCTCACTCCTCCGTTCTCTTCCTTCAGCAGCTCAAGCAAAGAAACTTTTTGGTGACAATTGGAGACGATGAACAAATCGCGGCGCAGCAATCAAGGTGCCTCAAGGTCTTTGACCTAGATAGGATACAGCCCGAGGGTTCGAGCTCGACGAGCCCCGATTGCATTGGCATCTTGAGGATTTTTACCAACCAATTTCCTGAAGCAAAGATTACATCGTTTTTAGTACTAGAGGAAGCGCCGCCGATACTACTTATAGCTATTGGATTAGATAATGGCTGCATTTACTGCATTAAAGGGGATATTGCACGGGAGCGTATCACCCGGTTCAAGCTCCAGGTGAATAATGTTTCTGACAAGAGCCAGTGCTCGATCACAGGATTGGGGTTCAGAGTGGATGGTCAGGCCCTTCAGTTGTTTGCTGTGACACCGTCTTCTGTGAGCTTGTTCATCTTGCAGAATCAACCGTCAAGGGGGCAAACCCTAGATCAGATTGGAGGCAATGTTAACAGTGTGGCCATGAGTGATCGTGGGGAGTTGATAATTGGTCGACCTGAGGCTGTGTATTTTTATGAAGTTGATGGGCGTGGTCCTTGTTGGGCTTTTGAAGGAGAAAAGAAATTTTTAGGCTGGTTTCGTGGATATCTTCTGTGTATTATAGAAGATCAGAGAAGTGGTAACCACACTTTCAACATATATGACCTGAAGAACCATTTAATTGCCCACAGCCTTGTGGTAAAAGAGGTTTCTCACATGCTCTGCGAATGGGGTAGCATAATACTTATAATGGCTGACAAGTCCGCTTTGTGCATTGGGGAGAAGGATATGGAGAGCAAGTTAGATATGCTTTTTAAGAAGAATCTATATACGGTTGCCATCAACCTTGTCCAGAGTCAGCAAGCTGATGCTGCCGCAACTGCTGAAGTGCTAAGAAAGTATGGTGATCATCTTTATAGCAAGCAAGATTATGATGAGGCAATGGCCCAGTATATTCATACCATTGGTCACCTTGAGCCATCTTATGTTATACAAAAGTTTCTAGATGCGCAACGAATCTACAATCTTACGAATTACTTGGAGAAATTACATGAGAAGGGGCTTGCTTCTAAAGATCATACCACTCTTCTATTGAACTGCTACACCAAACTGAAAGATGTTGACAAGCTAAATGTGTTCATTAAAAGTGAGGATGGTCTTGGGGAACATAGGTTTGATGTGGAGACGGCAATACGGGTCTGCCGTGCTACCAACTACCATGAGCATGCAATGTATGTTGCTAAGAAGGCAGGGAAGCATGAGTGGTACTTGAAGATCTTACTTGAAGACCTTGGTAGATATGAGGAAGCCTTGCAATATATTTCAAGCCTTGAGCCAAGCCAAGCTGGAGTAACAGTCAAAGAGTACGGTAAGATTCTGATAGAGCACAAGCCCGAGGAGACAATTGAGATACTGATGAAGCTGTGCACTGAGGATGGAGAATCAGCCAAGAGAGGACGCACTAATGGTGCATACTTAACTATGTTACCATCTCCTGTTGATTTCCTGAACATCTTCATCCATCATCTTCAGTCACTTATGGTTTTCCTTGAAAAGTATACCAACAAGGTCAAAGACTCTCCTGCTCAAGTAGAAATCCATAACACACTCTTGGAGTTGTATTTGTCGAATGACTTGAACTTCCCGTTGATGTCGCAAGCTAGCAATGGTGGAGATATAAGTGTTAGGGCTATTAGACCAGGAGCAGCCACAATGTCAAAAGCTGAGTTGAATGGGAAATTTGTTGCTGATGGCAAAGACTTAACTCAGGAAATGGACCGTATGGAAAGGCAAGAGAAAGGACTAAGACTGCTAAAGAGTGCATGGCCGTCTGAGCTGGAACATCCGCTTTATGATGTTGATCTTGCCATAATCCTCTGTGAAATGAATGAATTTAAGGAAGGGCTTCTCTATATATATGAGAAAATGAAACTCTATAAAGAGGTGATTTCTTGCTACATGCAAGCCCATGACCATGAAGGTTTGATTGCATGCTGCAAACGATTAGGGGATTCAGGTAAGGGTGGGGACCCAACTCTTTGGGCAGATCTGCTGAAATATTTTGGTGAACTTGGAGAAGATTGTTCCAAAGAAGTGAAGGAAGTTTTGACATATATAGAAAGAGATGATATCTTGCCTCCTATTATTGTTCTTCAAACACTGTCCAGAAATCCATGCCTCACACTCTCTGTCATTAAGGAGTACATTGCTCGAAAGCTTGAACAAGAATCAAAACTGATTGAAGAGGACCGTCGAGCTATTGAGAAGTATCAGGAAGCAACATTGACAATGAGAAAAGAAATTCAGGATCTTAGGACAAATGCAAGGATCTTTCAGCTCAGCAAGTGCACTGCATGTACTTTCACTCTTGATCTCCCTGCTGTACATTTTATGTGTATCCACTCATTCCATCAGCGTTGCCTTGGTGATAATGAGAAAGAATGCCCCGTATGTGCTCCTGAATACAGATCTGTTCTAGAAATGAAAACAAGTTTAGAGCAAAACTCCAAAGATCAGGATCGGTTCTTCCAGCAAGTTAAAAATTCAAAGGATGGTTTTTCTGTAATCGCTGAGTATTTCGGGAAGGGGGTCATTAGCAAAACCAGTAATGGACCCACAGGTGCTTCTTCTTTGATCAGCAGCAACAGCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000323 GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005768 GO:0005769 GO:0005770 GO:0005773 GO:0005774 GO:0005856 GO:0005884 GO:0006810 GO:0006887 GO:0006904 GO:0006906 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0008150 GO:0008333 GO:0009705 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010256 GO:0010604 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019222 GO:0019904 GO:0019905 GO:0022406 GO:0030139 GO:0030674 GO:0030897 GO:0031090 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031338 GO:0031410 GO:0031982 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032879 GO:0032940 GO:0032991 GO:0033043 GO:0033263 GO:0034058 GO:0035542 GO:0042144 GO:0042176 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046903 GO:0046907 GO:0048278 GO:0048284 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051049 GO:0051050 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051640 GO:0051641 GO:0051649 GO:0060090 GO:0060255 GO:0060341 GO:0060627 GO:0061024 GO:0061025 GO:0065007 GO:0071840 GO:0080090 GO:0080171 GO:0090174 GO:0097576 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140029 GO:0140056 GO:1901998 GO:1902115 GO:1903362 GO:1903364 GO:1903649 GO:1903651 GO:2000641 GO:2000643
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin VPS11_C zf-C3H2C3

Protein Analysis

956

Amino Acids

108.22

Weight (kDa)

5.73

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEP5_VPS11_N PF23341 4 - 333 1.6e-90 PEP5/VPS11 N-terminal
VPS11_N PF23266 26 - 328 4.7e-116 VPS11-like, N-terminal beta-propeller
TPR_PEP5_VPS11 PF23356 378 - 618 1.6e-62 PEP5/VPS11 Clathrin repeat
Clathrin PF00637 398 - 537 1.4e-06 Region in Clathrin and VPS
TPR_PEP5_VPS11 PF23356 687 - 791 1.4e-17 PEP5/VPS11 Clathrin repeat
zf-C3H2C3 PF17122 855 - 890 6.8e-06 Zinc-finger
VPS11_C PF12451 895 - 937 1e-13 Vacuolar protein sorting protein 11 C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012419)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05170
fragaria_vesca FvH4_6g40910 FvH4_6g40910
malus_domestica MD09G1123500.v1.1 MD17G1113900.v1.1
prunus_persica Prupe.3G200600_v2.0.a1
pyrus_communis pycom09g04750 pycom17g10560
rosa_chinensis RchiOBHm_Chr2g0156491
rosa_laevigata RLG00000020917
rosa_multiflora Rmu_co8501807.1_g000001 Rmu_sc0005392.1_g000009
rosa_roxburghii Rroxscaffold_2G00092980
rosa_rugosa Rorug02G0453700
rosa_samantha Rh2AG519800 Rh2BG532100 Rh2CG504800 Rh2DG541100
rosa_wichuraiana Rw2G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 965
AasI GACNNNNNNGTC 1 cut(s) 137
Acc16I TGCGCA 1 cut(s) 1227
AccB1I GGYRCC 2 cut(s) 134, 279
AccBSI CCGCTC 1 cut(s) 99
AccI GTMKAC 2 cut(s) 730, 1775
AccII CGCG 2 cut(s) 56, 263
AciI CCGC 8 cut(s) 44, 99, 154, 263, 422, 981, 1092, 2063
AclWI GGATC 3 cut(s) 2532, 2552, 2742
AcoI YGGCCR 2 cut(s) 696, 2042
AcsI RAATTY 6 cut(s) 17, 800, 1955, 2108, 2517, 2758
AcuI CTGAAG 7 cut(s) 151, 191, 402, 575, 905, 1122, 1730
AfaI GTAC 7 cut(s) 5, 408, 1484, 1583, 2420, 2575, 2601
AfiI CCNNNNNNNGG 1 cut(s) 653
AjnI CCWGG 2 cut(s) 516, 1915
Alw21I GWGCWC 6 cut(s) 329, 551, 1605, 1646, 2567, 2674
Alw26I GTCTC 3 cut(s) 240, 1394, 1610
Alw44I GTGCAC 2 cut(s) 1642, 2563
AlwI GGATC 3 cut(s) 2532, 2552, 2742
Ama87I CYCGRG 3 cut(s) 62, 314, 1610
AoxI GGCC 4 cut(s) 585, 696, 1161, 2042
ApaLI GTGCAC 2 cut(s) 1642, 2563
ApeKI GCWGC 9 cut(s) 123, 156, 211, 268, 459, 1088, 1922, 2211, 2856
ApoI RAATTY 6 cut(s) 17, 800, 1955, 2108, 2517, 2758
Asp700I GAANNNNTTC 2 cut(s) 1730, 2121
AspLEI GCGC 4 cut(s) 56, 268, 421, 1228
AspS9I GGNCC 7 cut(s) 586, 770, 1162, 1993, 2245, 2464, 2828
AsuC2I CCSGG 1 cut(s) 505
AsuHPI GGTGA 9 cut(s) 127, 247, 493, 532, 1133, 1178, 2173, 2294, 2654
AsuNHI GCTAGC 1 cut(s) 1877
AvaI CYCGRG 3 cut(s) 62, 314, 1610
AvaII GGWCC 5 cut(s) 770, 1993, 2245, 2464, 2828
AxyI CCTNAGG 1 cut(s) 735
BaeGI GKGCMC 2 cut(s) 1646, 2567
BalI TGGCCA 1 cut(s) 698
BanI GGYRCC 2 cut(s) 134, 279
BanII GRGCYC 2 cut(s) 329, 338
BbsI GAAGAC 2 cut(s) 606, 1509
Bbv12I GWGCWC 6 cut(s) 329, 551, 1605, 1646, 2567, 2674
BbvI GCAGC 8 cut(s) 110, 168, 223, 280, 446, 1075, 1934, 2198
BceAI ACGGC 5 cut(s) 148, 190, 1404, 1419, 2029
BciT130I CCWGG 2 cut(s) 518, 1917
BciVI GTATCC 2 cut(s) 300, 2624
BclI TGATCA 2 cut(s) 1123, 2850
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 3 cut(s) 240, 1394, 1610
BfaI CTAG 6 cut(s) 299, 410, 665, 1220, 1878, 2693
BfoI RGCGCY 1 cut(s) 422
BfuI GTATCC 2 cut(s) 300, 2624
BglII AGATCT 3 cut(s) 1491, 2263, 2684
BlpI GCTNAGC 1 cut(s) 2555
BmcAI AGTACT 1 cut(s) 408
Bme1390I CCNGG 3 cut(s) 505, 518, 1917
Bme18I GGWCC 5 cut(s) 770, 1993, 2245, 2464, 2828
BmeT110I CYCGRG 3 cut(s) 62, 314, 1610
BmgT120I GGNCC 7 cut(s) 586, 770, 1162, 1993, 2245, 2464, 2828
BmiI GGNNCC 5 cut(s) 136, 281, 2246, 2247, 2830
BmrFI CCNGG 3 cut(s) 505, 518, 1917
BmrI ACTGGG 1 cut(s) 1159
BmsI GCATC 3 cut(s) 360, 1075, 1213
BmtI GCTAGC 1 cut(s) 1881
BmuI ACTGGG 1 cut(s) 1159
BpiI GAAGAC 2 cut(s) 606, 1509
BplI GAGNNNNNCTC 2 cut(s) 2705, 2737
BpmI CTGGAG 2 cut(s) 500, 1584
Bpu1102I GCTNAGC 1 cut(s) 2555
BpuEI CTTGAG 6 cut(s) 200, 269, 376, 1211, 1568, 1788
BpuMI CCSGG 1 cut(s) 505
BsaBI GATNNNNATC 2 cut(s) 2076, 2733
BsaJI CCNNGG 5 cut(s) 63, 315, 1507, 1611, 2638
BsaXI ACNNNNNCTCC 6 cut(s) 669, 699, 1001, 1031, 1557, 1587
Bsc4I CCNNNNNNNGG 1 cut(s) 653
Bse1I ACTGG 4 cut(s) 7, 544, 1165, 2820
Bse21I CCTNAGG 1 cut(s) 735
Bse3DI GCAATG 5 cut(s) 688, 1164, 1452, 1888, 2421
Bse8I GATNNNNATC 2 cut(s) 2076, 2733
BseBI CCWGG 2 cut(s) 518, 1917
BseDI CCNNGG 5 cut(s) 63, 315, 1507, 1611, 2638
BseGI GGATG 6 cut(s) 583, 1375, 1657, 1734, 2059, 2773
BseJI GATNNNNATC 2 cut(s) 2076, 2733
BseLI CCNNNNNNNGG 1 cut(s) 653
BseMI GCAATG 5 cut(s) 688, 1164, 1452, 1888, 2421
BseMII CTCAG 7 cut(s) 726, 1638, 1932, 1997, 2040, 2569, 2778
BseNI ACTGG 4 cut(s) 7, 544, 1165, 2820
BseRI GAGGAG 3 cut(s) 47, 181, 1628
BseSI GKGCMC 2 cut(s) 1646, 2567
BseXI GCAGC 8 cut(s) 110, 168, 223, 280, 446, 1075, 1934, 2198
Bsh1236I CGCG 2 cut(s) 56, 263
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 4 cut(s) 587, 698, 1163, 2044
BshNI GGYRCC 2 cut(s) 134, 279
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 6 cut(s) 329, 551, 1605, 1646, 2567, 2674
BsiHKCI CYCGRG 3 cut(s) 62, 314, 1610
BsiSI CCGG 1 cut(s) 505
BslFI GGGAC 1 cut(s) 2258
BslI CCNNNNNNNGG 1 cut(s) 653
BsmAI GTCTC 3 cut(s) 240, 1394, 1610
BsmBI CGTCTC 2 cut(s) 240, 1394
BsmFI GGGAC 1 cut(s) 2258
BsmI GAATGC 1 cut(s) 2663
BsnI GGCC 4 cut(s) 587, 698, 1163, 2044
BsoBI CYCGRG 3 cut(s) 62, 314, 1610
Bsp1286I GDGCHC 7 cut(s) 329, 338, 551, 1605, 1646, 2567, 2674
Bsp1407I TGTACA 1 cut(s) 2599
Bsp1720I GCTNAGC 1 cut(s) 2555
BspACI CCGC 8 cut(s) 44, 99, 154, 263, 422, 981, 1092, 2063
BspANI GGCC 4 cut(s) 587, 698, 1163, 2044
BspCNI CTCAG 7 cut(s) 727, 1639, 1933, 1996, 2041, 2568, 2779
BspFNI CGCG 2 cut(s) 56, 263
BspLI GGNNCC 5 cut(s) 136, 281, 2246, 2247, 2830
BspOI GCTAGC 1 cut(s) 1881
BspPI GGATC 3 cut(s) 2532, 2552, 2742
BspT107I GGYRCC 2 cut(s) 134, 279
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 5 cut(s) 688, 1164, 1452, 1888, 2421
BsrGI TGTACA 1 cut(s) 2599
BsrI ACTGG 4 cut(s) 7, 544, 1165, 2820
BssECI CCNNGG 5 cut(s) 63, 315, 1507, 1611, 2638
BssNAI GTATAC 1 cut(s) 1776
BssT1I CCWWGG 2 cut(s) 1507, 2638
Bst1107I GTATAC 1 cut(s) 1776
Bst2UI CCWGG 2 cut(s) 518, 1917
Bst6I CTCTTC 3 cut(s) 206, 1308, 2454
BstAPI GCANNNNNTGC 1 cut(s) 468
BstAUI TGTACA 1 cut(s) 2599
BstC8I GCNNGC 9 cut(s) 101, 1080, 1139, 1281, 1443, 1875, 1879, 2181, 2209
BstEII GGTNACC 2 cut(s) 857, 1184
BstF5I GGATG 6 cut(s) 583, 1375, 1657, 1734, 2059, 2773
BstFNI CGCG 2 cut(s) 56, 263
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 4 cut(s) 56, 268, 421, 1228
BstMAI GTCTC 3 cut(s) 240, 1394, 1610
BstMCI CGRYCG 1 cut(s) 154
BstNI CCWGG 2 cut(s) 518, 1917
BstNSI RCATGY 5 cut(s) 937, 1445, 2179, 2211, 2574
BstPI GGTNACC 2 cut(s) 857, 1184
BstSCI CCNGG 3 cut(s) 503, 516, 1915
BstSLI GKGCMC 2 cut(s) 1646, 2567
BstUI CGCG 2 cut(s) 56, 263
BstV1I GCAGC 8 cut(s) 110, 168, 223, 280, 446, 1075, 1934, 2198
BstV2I GAAGAC 2 cut(s) 606, 1509
BstX2I RGATCY 5 cut(s) 1491, 2263, 2524, 2544, 2684
BstXI CCANNNNNNTGG 1 cut(s) 2256
BstYI RGATCY 5 cut(s) 1491, 2263, 2524, 2544, 2684
BstZ17I GTATAC 1 cut(s) 1776
Bsu36I CCTNAGG 1 cut(s) 735
BsuI GTATCC 2 cut(s) 300, 2624
BsuRI GGCC 4 cut(s) 587, 698, 1163, 2044
BtsCI GGATG 6 cut(s) 583, 1375, 1657, 1734, 2059, 2773
BtsI GCAGTG 1 cut(s) 2565
BtsIMutI CAGTG 6 cut(s) 14, 551, 697, 1644, 2375, 2565
Cac8I GCNNGC 9 cut(s) 101, 1080, 1139, 1281, 1443, 1875, 1879, 2181, 2209
CfoI GCGC 4 cut(s) 56, 268, 421, 1228
Cfr13I GGNCC 7 cut(s) 586, 770, 1162, 1993, 2245, 2464, 2828
CseI GACGC 1 cut(s) 1683
Csp6I GTAC 7 cut(s) 4, 407, 1483, 1582, 2419, 2574, 2600
CviQI GTAC 7 cut(s) 4, 407, 1483, 1582, 2419, 2574, 2600
DrdI GACNNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 137
EaeI YGGCCR 2 cut(s) 696, 2042
Eam1104I CTCTTC 3 cut(s) 206, 1308, 2454
EarI CTCTTC 3 cut(s) 206, 1308, 2454
Ecl136II GAGCTC 1 cut(s) 327
Eco130I CCWWGG 2 cut(s) 1507, 2638
Eco24I GRGCYC 2 cut(s) 329, 338
Eco32I GATATC 2 cut(s) 824, 2347
Eco47I GGWCC 5 cut(s) 770, 1993, 2245, 2464, 2828
Eco53kI GAGCTC 1 cut(s) 327
Eco57I CTGAAG 7 cut(s) 151, 191, 402, 575, 905, 1122, 1730
Eco81I CCTNAGG 1 cut(s) 735
Eco88I CYCGRG 3 cut(s) 62, 314, 1610
Eco91I GGTNACC 2 cut(s) 857, 1184
EcoICRI GAGCTC 1 cut(s) 327
EcoO109I RGGNCCY 2 cut(s) 586, 2245
EcoO65I GGTNACC 2 cut(s) 857, 1184
EcoRII CCWGG 2 cut(s) 516, 1915
EcoRV GATATC 2 cut(s) 824, 2347
EcoT14I CCWWGG 2 cut(s) 1507, 2638
EcoT38I GRGCYC 2 cut(s) 329, 338
ErhI CCWWGG 2 cut(s) 1507, 2638
Esp3I CGTCTC 2 cut(s) 240, 1394
FaqI GGGAC 1 cut(s) 2258
FauI CCCGC 2 cut(s) 37, 92
FbaI TGATCA 2 cut(s) 1123, 2850
FblI GTMKAC 2 cut(s) 730, 1775
FokI GGATG 6 cut(s) 590, 1382, 1664, 1721, 2046, 2780
FriOI GRGCYC 2 cut(s) 329, 338
FspBI CTAG 6 cut(s) 299, 410, 665, 1220, 1878, 2693
FspI TGCGCA 1 cut(s) 1227
GlaI GCGC 4 cut(s) 55, 267, 420, 1227
GsuI CTGGAG 2 cut(s) 500, 1584
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 4 cut(s) 587, 698, 1163, 2044
HapII CCGG 1 cut(s) 505
HgaI GACGC 1 cut(s) 1683
HhaI GCGC 4 cut(s) 56, 268, 421, 1228
Hin6I GCGC 4 cut(s) 54, 266, 419, 1226
HinP1I GCGC 4 cut(s) 54, 266, 419, 1226
HincII GTYRAC 3 cut(s) 688, 731, 1339
HindII GTYRAC 3 cut(s) 688, 731, 1339
HindIII AAGCTT 1 cut(s) 2432
HpaI GTTAAC 1 cut(s) 688
HpaII CCGG 1 cut(s) 505
HphI GGTGA 9 cut(s) 127, 247, 493, 532, 1133, 1178, 2173, 2294, 2654
Hpy166II GTNNAC 7 cut(s) 688, 731, 1339, 1644, 1776, 2285, 2565
Hpy188I TCNGA 6 cut(s) 535, 572, 672, 849, 1596, 2050
Hpy8I GTNNAC 7 cut(s) 688, 731, 1339, 1644, 1776, 2285, 2565
Hpy99I CGWCG 4 cut(s) 131, 134, 334, 2472
HspAI GCGC 4 cut(s) 54, 266, 419, 1226
KflI GGGWCCC 1 cut(s) 2245
Ksp22I TGATCA 2 cut(s) 1123, 2850
KspAI GTTAAC 1 cut(s) 688
LmnI GCTCC 4 cut(s) 493, 519, 1919, 2677
Lsp1109I GCAGC 8 cut(s) 110, 168, 223, 280, 446, 1075, 1934, 2198
LweI GCATC 3 cut(s) 360, 1075, 1213
MaeI CTAG 6 cut(s) 299, 410, 665, 1220, 1878, 2693
MaeIII GTNAC 8 cut(s) 70, 235, 604, 857, 1184, 1567, 1701, 1749
MbiI CCGCTC 1 cut(s) 99
MfeI CAATTG 2 cut(s) 240, 1620
MflI RGATCY 5 cut(s) 1491, 2263, 2524, 2544, 2684
MhlI GDGCHC 7 cut(s) 329, 338, 551, 1605, 1646, 2567, 2674
MlsI TGGCCA 1 cut(s) 698
MluNI TGGCCA 1 cut(s) 698
MlyI GAGTC 2 cut(s) 1081, 1787
Mox20I TGGCCA 1 cut(s) 698
MroXI GAANNNNTTC 2 cut(s) 1730, 2121
MscI TGGCCA 1 cut(s) 698
MslI CAYNNNNRTG 2 cut(s) 1440, 2190
Msp20I TGGCCA 1 cut(s) 698
MspI CCGG 1 cut(s) 505
MspR9I CCNGG 3 cut(s) 505, 518, 1917
MunI CAATTG 2 cut(s) 240, 1620
Mva1269I GAATGC 1 cut(s) 2663
MvaI CCWGG 2 cut(s) 518, 1917
MvnI CGCG 2 cut(s) 56, 263
NciI CCSGG 1 cut(s) 505
NheI GCTAGC 1 cut(s) 1877
NlaIV GGNNCC 5 cut(s) 136, 281, 2246, 2247, 2830
NmuCI GTSAC 4 cut(s) 235, 604, 1184, 1749
NsbI TGCGCA 1 cut(s) 1227
NspI RCATGY 5 cut(s) 937, 1445, 2179, 2211, 2574
PaeI GCATGC 2 cut(s) 1445, 2211
PcsI WCGNNNNNNNCGW 5 cut(s) 27, 126, 135, 329, 1417
PctI GAATGC 1 cut(s) 2663
PdmI GAANNNNTTC 2 cut(s) 1730, 2121
PfeI GAWTC 8 cut(s) 58, 640, 1039, 1233, 1591, 1658, 2228, 2444
PflFI GACNNNGTC 1 cut(s) 610
PleI GAGTC 2 cut(s) 1080, 1787
PpsI GAGTC 2 cut(s) 1080, 1787
PpuMI RGGWCCY 1 cut(s) 2245
PsiI TTATAA 1 cut(s) 965
Psp124BI GAGCTC 1 cut(s) 329
Psp5II RGGWCCY 1 cut(s) 2245
Psp6I CCWGG 2 cut(s) 516, 1915
PspEI GGTNACC 2 cut(s) 857, 1184
PspGI CCWGG 2 cut(s) 516, 1915
PspN4I GGNNCC 5 cut(s) 136, 281, 2246, 2247, 2830
PspPI GGNCC 7 cut(s) 586, 770, 1162, 1993, 2245, 2464, 2828
PspPPI RGGWCCY 1 cut(s) 2245
PsuI RGATCY 5 cut(s) 1491, 2263, 2524, 2544, 2684
PsyI GACNNNGTC 1 cut(s) 610
RsaI GTAC 7 cut(s) 5, 408, 1484, 1583, 2420, 2575, 2601
RsaNI GTAC 7 cut(s) 4, 407, 1483, 1582, 2419, 2574, 2600
RseI CAYNNNNRTG 2 cut(s) 1440, 2190
SacI GAGCTC 1 cut(s) 329
SalI GTCGAC 1 cut(s) 729
Sau96I GGNCC 7 cut(s) 586, 770, 1162, 1993, 2245, 2464, 2828
ScaI AGTACT 1 cut(s) 408
SchI GAGTC 2 cut(s) 1081, 1787
ScrFI CCNGG 3 cut(s) 505, 518, 1917
SduI GDGCHC 7 cut(s) 329, 338, 551, 1605, 1646, 2567, 2674
SfaNI GCATC 3 cut(s) 360, 1075, 1213
SinI GGWCC 5 cut(s) 770, 1993, 2245, 2464, 2828
SmiMI CAYNNNNRTG 2 cut(s) 1440, 2190
SmlI CTYRAG 6 cut(s) 215, 284, 355, 1190, 1547, 1803
SmoI CTYRAG 6 cut(s) 215, 284, 355, 1190, 1547, 1803
SphI GCATGC 2 cut(s) 1445, 2211
SsiI CCGC 8 cut(s) 44, 99, 154, 263, 422, 981, 1092, 2063
SspI AATATT 1 cut(s) 2276
SspMI CTAG 6 cut(s) 299, 410, 665, 1220, 1878, 2693
SstI GAGCTC 1 cut(s) 329
StyD4I CCNGG 3 cut(s) 503, 516, 1915
StyI CCWWGG 2 cut(s) 1507, 2638
TatI WGTACW 4 cut(s) 406, 2418, 2573, 2599
TauI GCSGC 3 cut(s) 266, 424, 1094
TfiI GAWTC 8 cut(s) 58, 640, 1039, 1233, 1591, 1658, 2228, 2444
TscAI CASTG 6 cut(s) 14, 551, 697, 1651, 2382, 2572
TseFI GTSAC 4 cut(s) 235, 604, 1184, 1749
TseI GCWGC 9 cut(s) 123, 156, 211, 268, 459, 1088, 1922, 2211, 2856
Tsp45I GTSAC 4 cut(s) 235, 604, 1184, 1749
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 6 cut(s) 14, 551, 697, 1651, 2382, 2572
Tth111I GACNNNGTC 1 cut(s) 610
VneI GTGCAC 2 cut(s) 1642, 2563
VpaK11BI GGWCC 5 cut(s) 770, 1993, 2245, 2464, 2828
XapI RAATTY 6 cut(s) 17, 800, 1955, 2108, 2517, 2758
XbaI TCTAGA 2 cut(s) 1219, 2692
XceI RCATGY 5 cut(s) 937, 1445, 2179, 2211, 2574
XmiI GTMKAC 2 cut(s) 730, 1775
XmnI GAANNNNTTC 2 cut(s) 1730, 2121
XspI CTAG 6 cut(s) 299, 410, 665, 1220, 1878, 2693
ZrmI AGTACT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.