MD09G1123500.v1.1

Vacuolar protein-sorting-associated protein 11 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012419
Physical Location & Seq
Reverse (-)
9533707 .. 9538160
4454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1123500.v1.1.491

Sequence Viewer

Length: 2859 bp
ATGTACCAGTGGAGGAAATTCGAGTTCTTCGAGGAGAAATTGGCGGGAAAGTGCGCGATTCCCGAGGAGGTGGAAGGGAAGATCGAGTGTTGCTCGAGCGGCAGAGGGAAGGTGGTGATCGGCTGCGACGACGGCACCATCAGCTTCCTGGACCGCGGCCTCAACTTCAGCTACGGATTCCAAGCTCACTCCTCCTCCGCGCTCTTCCTTCAGCAGCTCAAGCAACGCAACTATTTGGTGACAATTGGAGAAGATGAACAAATTACTCCGCAGCAATCGGCAATGTGCCTCAAGGTTTTCGACCTGGATAGAATGCAGTCCGAGGGCACGAGCTCAAGCACAACGAGCCCTGATTGCATTGGAATCTTGCGGATATTCACCAACCAATTTCCTGAAGCAAAGATTACATCATTTTTGGTACTAGAGGAGGCACCTCCGATACTGCTTATAGCTATTGGCTTGGATAATGGTTCGATTTACTGCATTAAAGGGGATATTGCTCGAGAACGAATCACCCGGTTTAAGCTTCAGGTGGAAATCCATTCTGACAAGAGCCAGTCCTCAATTACGGGACTGGGGTTCAGAGTGGACGGTCAAGCTCTTCAGTTGTTTGCTGTAACTCCTTCCTCTGTGAGCTTGTTCATCTTGCAGAATCAACCCTCAAACACAAGGCGGCAGACCCTAGATCAGATTGGAAGTAATATGAACAGTGTTGCAATGAGTGATCGCTTGTTGATAATTGGTCGACCTGAGGCCGTGTATTTTTATGAAGTTGATGGGCGTGGACCATGTTGGGCTTTTGAAGGAGAAAAGAAATTTTTAGGGTGGTTTCGTGGATACCTTCTTTGTGTTATTGCAGATCAAAGAAATAACAGCAACACTTTCAACATTTATGACTTGAAGAACCGTTTAATCGCTCACAGCCTAGCGGTCAAAGAGGTTTCTCACATGCTTTGTGAATGGGGTAACATAATACTTATCATGGCTGACAAATCAGCTTTGTGCATTGGGGAGAAGGATATGGAGAGCAAGTTAGATATGCTCTTTAAGAAGAATCTTTATACTGTTGCCATCAACCTTGTTCAAAGTCAGCAAGCGGATGCTGCCGCAACTGCAGAAGTGCTGAGAAAGTATGGGGATCATCTATATAGCAAGCAAGATTATGATGAGGCAATGGCACAGTATATCCATACCATTGGTCACCTCGAGCCTTCTTATGTGATACAGAAGTTTCTAGATGCACAACGAATCTACAACCTTACAAATTACTTGGAAAAATTGCATGAGAAGGGGCTTGCTTCCAAAGATCATACCACTCTTCTACTGAATTGCTATACGAAATTGAAAGATGTTGACAAACTAAATGTGTTCATTAAAAGTGAGGATGGTGTTGGGGAACATAAATTTGATGTGGAGACTGCAATAAGAGTCTGCCGTGCCACCAACTACCATGAGCATGCAATGTATGTTGCTAAAAAGGCTGGGAAGCACGAATGGTACTTAAAGATCTTACTTGAAGATCTTGGCCGATATGAGGAAGCTTTGCAATATATTTCAAGCCTTGAACCAAGCCAAGCTGGAGTGACAGTGAAAGAGTATGGTAAGATTCTTATAGAGCACAAGCCTGTGGAAACAATTGAGATACTCATGAGGCTGTGCACTGAGGATGGTGAATCATCCAAGAGAGGAGCCGCTAATGGTTCATACCTGACAATGTTGCCATCTCCGGTTGATTTTCTCAACATCTTCACCCATCATCTTCCCTGGCTTATGGATTTTCTTGAAAAATATACCGACAAGGTCAAGGACTCTCCTGCACAAGTAGAAATCCATAACACGCTCTTGGAGTTGTACTTGTCCACTGACTTGAGCTTCCCATCAATATCACAAGCTAGCAATGGTGAAGATCTTAATCTCAGGGCTAGATCAGGTGCAGCTGCAACATCAAGAAGCCAGTCCAATGGGAAATTGATTGCTGATACCAAAGATTCAAACAAGGAAAAGGACCGTTTTGAAAAGCAACAGAAAGGGCTACGACTTCTTAAGAGTGCATGGCCATCTGACCTGGAATTTCCGCTTTATGATGTTGACCTTGCCGTAATTCTTTGTGAAATGAATGCATTTAAGGAAGGCCTGTTGTATCTCTATGAAAAATTGAAACTGTATAAAGAGGTGATTGCTTGCTACATGCAAGCTCATGACCATGAGGGTTTGATTGATTGCTGCAAACGACTAGGGGATTCAGGGAAGGGTGGGGACCCCACTCTTTGGGCAGATCTGCTAAAATATTTTGGTGAACTTGGAGAAGATTGCTCCAAAGAAGTGAAGGAAGTTTTGACATATATAGAAAGGGATGACATATTGCCTCCTATTATTGTTCTTCAAACTTTGTCCAGAAATCCATGTCTCACACTCTCTGTCATTAAGGATTACATTGCCAGAAAGCTTGAACAAGAATCAAAGCTGATCGAAGAGGACCGTCGAGCAATTGAAAAGTATCAGGAAACCACATCTGCAATGAGAAACGAAATTCAGGATCTTAGGACAAATGCCAGAATCTTTCAGCTCAGCAAGTGCACTGCATGCACTTTCACCCTCGATCTCCCAGCTGTGCATTTTATGTGTATCCACTCGTTCCATCAGCGTTGCCTCGGGGATAATGAGAAGGAGTGTCCTGTATGTGCTCCTGAATACAAATCTGTTCTCGAAACAAAAAGAAGTCTAGAGCAGAACTCCAAAGATCAGGATCGGTTCTTCCAGCAAGTTAAAAGTTCAAAGGATGGTTTTTCTGTGATTGCCGAGTATTTTGGGAAGGGGGTGATCAGCAAAACCACTAATGGACCCACAGGTGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000323 GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005768 GO:0005769 GO:0005770 GO:0005773 GO:0005774 GO:0005856 GO:0005884 GO:0006810 GO:0006887 GO:0006904 GO:0006906 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0008150 GO:0008333 GO:0009705 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010256 GO:0010604 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019222 GO:0019904 GO:0019905 GO:0022406 GO:0030139 GO:0030674 GO:0030897 GO:0031090 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031338 GO:0031410 GO:0031982 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032879 GO:0032940 GO:0032991 GO:0033043 GO:0033263 GO:0034058 GO:0035542 GO:0042144 GO:0042176 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046903 GO:0046907 GO:0048278 GO:0048284 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051049 GO:0051050 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051640 GO:0051641 GO:0051649 GO:0060090 GO:0060255 GO:0060341 GO:0060627 GO:0061024 GO:0061025 GO:0065007 GO:0071840 GO:0080090 GO:0080171 GO:0090174 GO:0097576 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140029 GO:0140056 GO:1901998 GO:1902115 GO:1903362 GO:1903364 GO:1903649 GO:1903651 GO:2000641 GO:2000643
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin VPS11_C zf-C3H2C3

Protein Analysis

953

Amino Acids

107.78

Weight (kDa)

5.72

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEP5_VPS11_N PF23341 4 - 338 3.8e-93 PEP5/VPS11 N-terminal
VPS11_N PF23266 25 - 333 1.8e-117 VPS11-like, N-terminal beta-propeller
TPR_PEP5_VPS11 PF23356 383 - 622 6.6e-62 PEP5/VPS11 Clathrin repeat
Clathrin PF00637 403 - 542 1.9e-06 Region in Clathrin and VPS
TPR_PEP5_VPS11 PF23356 662 - 795 1.6e-17 PEP5/VPS11 Clathrin repeat
zf-C3H2C3 PF17122 859 - 894 6.8e-06 Zinc-finger
VPS11_C PF12451 899 - 941 3.9e-14 Vacuolar protein sorting protein 11 C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012419)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05170
fragaria_vesca FvH4_6g40910 FvH4_6g40910
malus_domestica MD09G1123500.v1.1 MD17G1113900.v1.1
prunus_persica Prupe.3G200600_v2.0.a1
pyrus_communis pycom09g04750 pycom17g10560
rosa_chinensis RchiOBHm_Chr2g0156491
rosa_laevigata RLG00000020917
rosa_multiflora Rmu_co8501807.1_g000001 Rmu_sc0005392.1_g000009
rosa_roxburghii Rroxscaffold_2G00092980
rosa_rugosa Rorug02G0453700
rosa_samantha Rh2AG519800 Rh2BG532100 Rh2CG504800 Rh2DG541100
rosa_wichuraiana Rw2G042850

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 134, 430
AccB7I CCANNNNNTGG 1 cut(s) 2268
AccBSI CCGCTC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 745
AccII CGCG 3 cut(s) 56, 156, 200
AclWI GGATC 3 cut(s) 1146, 2544, 2754
AcoI YGGCCR 2 cut(s) 1525, 2054
AcsI RAATTY 5 cut(s) 17, 815, 1403, 2069, 2529
AcuI CTGAAG 5 cut(s) 151, 194, 414, 512, 587
AfaI GTAC 4 cut(s) 5, 420, 1499, 1853
AfiI CCNNNNNNNGG 2 cut(s) 1534, 2268
AflII CTTAAG 1 cut(s) 2042
AjnI CCWGG 4 cut(s) 147, 303, 1763, 2064
Alw21I GWGCWC 5 cut(s) 335, 1620, 1661, 2579, 2686
Alw26I GTCTC 2 cut(s) 1409, 2411
Alw44I GTGCAC 2 cut(s) 1657, 2575
AlwI GGATC 3 cut(s) 1146, 2544, 2754
Ama87I CYCGRG 5 cut(s) 62, 94, 501, 1205, 2651
AoxI GGCC 5 cut(s) 157, 753, 1525, 2054, 2131
ApaLI GTGCAC 2 cut(s) 1657, 2575
ApeKI GCWGC 7 cut(s) 123, 214, 271, 1103, 1934, 1937, 2223
ApoI RAATTY 5 cut(s) 17, 815, 1403, 2069, 2529
AspLEI GCGC 2 cut(s) 56, 202
AspS9I GGNCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
AsuC2I CCSGG 1 cut(s) 517
AsuNHI GCTAGC 1 cut(s) 1892
AvaI CYCGRG 5 cut(s) 62, 94, 501, 1205, 2651
AvaII GGWCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
AxyI CCTNAGG 1 cut(s) 750
BaeGI GKGCMC 3 cut(s) 329, 1661, 2579
BaeI ACNNNNGTAYC 2 cut(s) 1214, 1247
BalI TGGCCA 1 cut(s) 2056
BanI GGYRCC 2 cut(s) 134, 430
BanII GRGCYC 2 cut(s) 335, 350
BauI CACGAG 1 cut(s) 328
Bbv12I GWGCWC 5 cut(s) 335, 1620, 1661, 2579, 2686
BbvI GCAGC 7 cut(s) 110, 226, 283, 1090, 1924, 1946, 2210
BceAI ACGGC 4 cut(s) 148, 740, 1419, 2081
BciT130I CCWGG 4 cut(s) 149, 305, 1765, 2066
BciVI GTATCC 2 cut(s) 830, 2636
BclI TGATCA 1 cut(s) 2820
BcnI CCSGG 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 1409, 2411
BfaI CTAG 8 cut(s) 422, 683, 926, 1235, 1893, 1923, 2234, 2723
BfmI CTRYAG 1 cut(s) 1113
BfrI CTTAAG 1 cut(s) 2042
BfuI GTATCC 2 cut(s) 830, 2636
BglII AGATCT 4 cut(s) 1506, 1519, 1906, 2275
BlpI GCTNAGC 1 cut(s) 2567
Bme1390I CCNGG 5 cut(s) 149, 305, 517, 1765, 2066
Bme18I GGWCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
BmeT110I CYCGRG 5 cut(s) 62, 94, 501, 1205, 2651
BmgT120I GGNCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
BmiI GGNNCC 6 cut(s) 136, 432, 1690, 2258, 2259, 2842
BmrFI CCNGG 5 cut(s) 149, 305, 517, 1765, 2066
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 2 cut(s) 1090, 1228
BmtI GCTAGC 1 cut(s) 1896
BmuI ACTGGG 1 cut(s) 584
BplI GAGNNNNNCTC 4 cut(s) 77, 109, 2717, 2749
BpmI CTGGAG 1 cut(s) 1599
Bpu1102I GCTNAGC 1 cut(s) 2567
BpuEI CTTGAG 4 cut(s) 203, 275, 319, 1888
BpuMI CCSGG 1 cut(s) 517
BsaBI GATNNNNATC 2 cut(s) 1911, 2745
BsaJI CCNNGG 5 cut(s) 63, 154, 321, 1763, 2650
BsaWI WCCGGW 1 cut(s) 1726
BsaXI ACNNNNNCTCC 6 cut(s) 179, 209, 1016, 1046, 1572, 1602
Bsc4I CCNNNNNNNGG 2 cut(s) 1534, 2268
Bse1I ACTGG 4 cut(s) 7, 556, 579, 1954
Bse21I CCTNAGG 1 cut(s) 750
Bse3DI GCAATG 7 cut(s) 288, 723, 1179, 1467, 1903, 2433, 2523
Bse8I GATNNNNATC 2 cut(s) 1911, 2745
BseBI CCWGG 4 cut(s) 149, 305, 1765, 2066
BseDI CCNNGG 5 cut(s) 63, 154, 321, 1763, 2650
BseGI GGATG 6 cut(s) 1105, 1390, 1672, 1676, 2359, 2785
BseJI GATNNNNATC 2 cut(s) 1911, 2745
BseLI CCNNNNNNNGG 2 cut(s) 1534, 2268
BseMI GCAATG 7 cut(s) 288, 723, 1179, 1467, 1903, 2433, 2523
BseMII CTCAG 5 cut(s) 741, 1115, 1653, 1930, 2581
BseNI ACTGG 4 cut(s) 7, 556, 579, 1954
BseRI GAGGAG 6 cut(s) 47, 80, 181, 184, 440, 1701
BseSI GKGCMC 3 cut(s) 329, 1661, 2579
BseXI GCAGC 7 cut(s) 110, 226, 283, 1090, 1924, 1946, 2210
BseYI CCCAGC 2 cut(s) 1481, 2605
BsgI GTGCAG 2 cut(s) 1800, 1953
Bsh1236I CGCG 3 cut(s) 56, 156, 200
BshFI GGCC 5 cut(s) 159, 755, 1527, 2056, 2133
BshNI GGYRCC 2 cut(s) 134, 430
BsiHKAI GWGCWC 5 cut(s) 335, 1620, 1661, 2579, 2686
BsiHKCI CYCGRG 5 cut(s) 62, 94, 501, 1205, 2651
BsiSI CCGG 2 cut(s) 517, 1727
BslFI GGGAC 2 cut(s) 585, 2270
BslI CCNNNNNNNGG 2 cut(s) 1534, 2268
BsmAI GTCTC 2 cut(s) 1409, 2411
BsmFI GGGAC 2 cut(s) 585, 2270
BsmI GAATGC 2 cut(s) 318, 2122
BsnI GGCC 5 cut(s) 159, 755, 1527, 2056, 2133
BsoBI CYCGRG 5 cut(s) 62, 94, 501, 1205, 2651
Bsp1286I GDGCHC 7 cut(s) 329, 335, 350, 1620, 1661, 2579, 2686
Bsp1720I GCTNAGC 1 cut(s) 2567
BspANI GGCC 5 cut(s) 159, 755, 1527, 2056, 2133
BspCNI CTCAG 5 cut(s) 742, 1116, 1654, 1929, 2580
BspFNI CGCG 3 cut(s) 56, 156, 200
BspHI TCATGA 2 cut(s) 1647, 2197
BspLI GGNNCC 6 cut(s) 136, 432, 1690, 2258, 2259, 2842
BspMAI CTGCAG 1 cut(s) 1117
BspOI GCTAGC 1 cut(s) 1896
BspPI GGATC 3 cut(s) 1146, 2544, 2754
BspQI GCTCTTC 2 cut(s) 209, 606
BspT107I GGYRCC 2 cut(s) 134, 430
BspTI CTTAAG 1 cut(s) 2042
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 7 cut(s) 288, 723, 1179, 1467, 1903, 2433, 2523
BsrI ACTGG 4 cut(s) 7, 556, 579, 1954
BssECI CCNNGG 5 cut(s) 63, 154, 321, 1763, 2650
BssSI CACGAG 1 cut(s) 328
Bst2BI CACGAG 1 cut(s) 328
Bst2UI CCWGG 4 cut(s) 149, 305, 1765, 2066
Bst4CI ACNGT 9 cut(s) 593, 710, 908, 1066, 1182, 1588, 2009, 2163, 2480
Bst6I CTCTTC 4 cut(s) 209, 606, 1323, 2466
BstAFI CTTAAG 1 cut(s) 2042
BstAPI GCANNNNNTGC 1 cut(s) 2583
BstC8I GCNNGC 8 cut(s) 1095, 1154, 1296, 1458, 1894, 2182, 2193, 2584
BstDEI CTNAG 6 cut(s) 750, 1124, 1662, 1916, 2540, 2567
BstDSI CCRYGG 1 cut(s) 154
BstEII GGTNACC 1 cut(s) 1199
BstF5I GGATG 6 cut(s) 1105, 1390, 1672, 1676, 2359, 2785
BstFNI CGCG 3 cut(s) 56, 156, 200
BstHHI GCGC 2 cut(s) 56, 202
BstMAI GTCTC 2 cut(s) 1409, 2411
BstNI CCWGG 4 cut(s) 149, 305, 1765, 2066
BstNSI RCATGY 4 cut(s) 952, 1460, 2191, 2586
BstPI GGTNACC 1 cut(s) 1199
BstSCI CCNGG 5 cut(s) 147, 303, 515, 1763, 2064
BstSFI CTRYAG 1 cut(s) 1113
BstSLI GKGCMC 3 cut(s) 329, 1661, 2579
BstUI CGCG 3 cut(s) 56, 156, 200
BstV1I GCAGC 7 cut(s) 110, 226, 283, 1090, 1924, 1946, 2210
BstX2I RGATCY 5 cut(s) 1506, 1519, 1906, 2275, 2536
BstXI CCANNNNNNTGG 2 cut(s) 1196, 1961
BstYI RGATCY 5 cut(s) 1506, 1519, 1906, 2275, 2536
Bsu36I CCTNAGG 1 cut(s) 750
BsuI GTATCC 2 cut(s) 830, 2636
BsuRI GGCC 5 cut(s) 159, 755, 1527, 2056, 2133
BtgI CCRYGG 1 cut(s) 154
BtsCI GGATG 6 cut(s) 1105, 1390, 1672, 1676, 2359, 2785
BtsI GCAGTG 1 cut(s) 2577
BtsIMutI CAGTG 6 cut(s) 14, 715, 1593, 1659, 1860, 2577
Cac8I GCNNGC 8 cut(s) 1095, 1154, 1296, 1458, 1894, 2182, 2193, 2584
CciI TCATGA 2 cut(s) 1647, 2197
CfoI GCGC 2 cut(s) 56, 202
Cfr13I GGNCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
Cfr42I CCGCGG 1 cut(s) 157
Csp6I GTAC 4 cut(s) 4, 419, 1498, 1852
CviQI GTAC 4 cut(s) 4, 419, 1498, 1852
DdeI CTNAG 6 cut(s) 750, 1124, 1662, 1916, 2540, 2567
EaeI YGGCCR 2 cut(s) 1525, 2054
Eam1104I CTCTTC 4 cut(s) 209, 606, 1323, 2466
EarI CTCTTC 4 cut(s) 209, 606, 1323, 2466
Ecl136II GAGCTC 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 2133
Eco24I GRGCYC 2 cut(s) 335, 350
Eco47I GGWCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
Eco53kI GAGCTC 1 cut(s) 333
Eco57I CTGAAG 5 cut(s) 151, 194, 414, 512, 587
Eco81I CCTNAGG 1 cut(s) 750
Eco88I CYCGRG 5 cut(s) 62, 94, 501, 1205, 2651
Eco91I GGTNACC 1 cut(s) 1199
EcoICRI GAGCTC 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 2257
EcoO65I GGTNACC 1 cut(s) 1199
EcoRII CCWGG 4 cut(s) 147, 303, 1763, 2064
EcoT22I ATGCAT 1 cut(s) 2122
EcoT38I GRGCYC 2 cut(s) 335, 350
FaqI GGGAC 2 cut(s) 585, 2270
FauI CCCGC 1 cut(s) 37
FbaI TGATCA 1 cut(s) 2820
FblI GTMKAC 1 cut(s) 745
FokI GGATG 6 cut(s) 1112, 1397, 1663, 1679, 2366, 2792
FriOI GRGCYC 2 cut(s) 335, 350
FspBI CTAG 8 cut(s) 422, 683, 926, 1235, 1893, 1923, 2234, 2723
GlaI GCGC 2 cut(s) 55, 201
GsaI CCCAGC 2 cut(s) 1485, 2609
GsuI CTGGAG 1 cut(s) 1599
HaeIII GGCC 5 cut(s) 159, 755, 1527, 2056, 2133
HapII CCGG 2 cut(s) 517, 1727
HhaI GCGC 2 cut(s) 56, 202
Hin6I GCGC 2 cut(s) 54, 200
HinP1I GCGC 2 cut(s) 54, 200
HincII GTYRAC 3 cut(s) 746, 1354, 2089
HindII GTYRAC 3 cut(s) 746, 1354, 2089
HindIII AAGCTT 3 cut(s) 524, 1539, 2444
HpaII CCGG 2 cut(s) 517, 1727
Hpy166II GTNNAC 9 cut(s) 589, 746, 785, 1354, 1659, 1860, 2089, 2297, 2577
Hpy188I TCNGA 6 cut(s) 322, 438, 547, 584, 690, 2062
Hpy8I GTNNAC 9 cut(s) 589, 746, 785, 1354, 1659, 1860, 2089, 2297, 2577
Hpy99I CGWCG 3 cut(s) 131, 134, 2484
HpyCH4III ACNGT 9 cut(s) 593, 710, 908, 1066, 1182, 1588, 2009, 2163, 2480
HpyF3I CTNAG 6 cut(s) 750, 1124, 1662, 1916, 2540, 2567
HspAI GCGC 2 cut(s) 54, 200
KflI GGGWCCC 1 cut(s) 2257
Ksp22I TGATCA 1 cut(s) 2820
KspI CCGCGG 1 cut(s) 157
LguI GCTCTTC 2 cut(s) 209, 606
LmnI GCTCC 3 cut(s) 1688, 2318, 2689
Lsp1109I GCAGC 7 cut(s) 110, 226, 283, 1090, 1924, 1946, 2210
LweI GCATC 2 cut(s) 1090, 1228
MaeI CTAG 8 cut(s) 422, 683, 926, 1235, 1893, 1923, 2234, 2723
MaeIII GTNAC 5 cut(s) 238, 616, 965, 1199, 1582
MbiI CCGCTC 1 cut(s) 99
MfeI CAATTG 3 cut(s) 243, 1635, 2487
MflI RGATCY 5 cut(s) 1506, 1519, 1906, 2275, 2536
MhlI GDGCHC 7 cut(s) 329, 335, 350, 1620, 1661, 2579, 2686
MlsI TGGCCA 1 cut(s) 2056
MluNI TGGCCA 1 cut(s) 2056
MlyI GAGTC 2 cut(s) 1437, 1802
Mox20I TGGCCA 1 cut(s) 2056
Mph1103I ATGCAT 1 cut(s) 2122
MscI TGGCCA 1 cut(s) 2056
MslI CAYNNNNRTG 2 cut(s) 1455, 2202
Msp20I TGGCCA 1 cut(s) 2056
MspA1I CMGCKG 3 cut(s) 156, 1937, 2609
MspCI CTTAAG 1 cut(s) 2042
MspI CCGG 2 cut(s) 517, 1727
MspR9I CCNGG 5 cut(s) 149, 305, 517, 1765, 2066
MunI CAATTG 3 cut(s) 243, 1635, 2487
Mva1269I GAATGC 2 cut(s) 318, 2122
MvaI CCWGG 4 cut(s) 149, 305, 1765, 2066
MvnI CGCG 3 cut(s) 56, 156, 200
NciI CCSGG 1 cut(s) 517
NheI GCTAGC 1 cut(s) 1892
NlaIV GGNNCC 6 cut(s) 136, 432, 1690, 2258, 2259, 2842
NmeAIII GCCGAG 1 cut(s) 2824
NmuCI GTSAC 3 cut(s) 238, 1199, 1582
NsiI ATGCAT 1 cut(s) 2122
NspI RCATGY 4 cut(s) 952, 1460, 2191, 2586
PaeI GCATGC 2 cut(s) 1460, 2586
PaeR7I CTCGAG 3 cut(s) 94, 501, 1205
PagI TCATGA 2 cut(s) 1647, 2197
PceI AGGCCT 1 cut(s) 2133
PciSI GCTCTTC 2 cut(s) 209, 606
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 126
PctI GAATGC 2 cut(s) 318, 2122
PflFI GACNNNGTC 1 cut(s) 1799
PflMI CCANNNNNTGG 1 cut(s) 2268
PfoI TCCNGGA 1 cut(s) 147
PleI GAGTC 2 cut(s) 1436, 1802
PpsI GAGTC 2 cut(s) 1436, 1802
PpuMI RGGWCCY 1 cut(s) 2257
Psp124BI GAGCTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 2257
Psp6I CCWGG 4 cut(s) 147, 303, 1763, 2064
PspEI GGTNACC 1 cut(s) 1199
PspFI CCCAGC 2 cut(s) 1481, 2605
PspGI CCWGG 4 cut(s) 147, 303, 1763, 2064
PspN4I GGNNCC 6 cut(s) 136, 432, 1690, 2258, 2259, 2842
PspPI GGNCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
PspPPI RGGWCCY 1 cut(s) 2257
PspXI VCTCGAGB 2 cut(s) 94, 1205
PstI CTGCAG 1 cut(s) 1117
PsuI RGATCY 5 cut(s) 1506, 1519, 1906, 2275, 2536
PsyI GACNNNGTC 1 cut(s) 1799
PvuII CAGCTG 2 cut(s) 1937, 2609
RsaI GTAC 4 cut(s) 5, 420, 1499, 1853
RsaNI GTAC 4 cut(s) 4, 419, 1498, 1852
RseI CAYNNNNRTG 2 cut(s) 1455, 2202
SacI GAGCTC 1 cut(s) 335
SacII CCGCGG 1 cut(s) 157
SalI GTCGAC 1 cut(s) 744
SapI GCTCTTC 2 cut(s) 209, 606
Sau96I GGNCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
SchI GAGTC 2 cut(s) 1437, 1802
ScrFI CCNGG 5 cut(s) 149, 305, 517, 1765, 2066
SduI GDGCHC 7 cut(s) 329, 335, 350, 1620, 1661, 2579, 2686
SfaNI GCATC 2 cut(s) 1090, 1228
SfcI CTRYAG 1 cut(s) 1113
Sfr274I CTCGAG 3 cut(s) 94, 501, 1205
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
SlaI CTCGAG 3 cut(s) 94, 501, 1205
SmiMI CAYNNNNRTG 2 cut(s) 1455, 2202
SmlI CTYRAG 8 cut(s) 94, 218, 290, 334, 501, 1205, 1867, 2042
SmoI CTYRAG 8 cut(s) 94, 218, 290, 334, 501, 1205, 1867, 2042
SphI GCATGC 2 cut(s) 1460, 2586
SseBI AGGCCT 1 cut(s) 2133
SspI AATATT 1 cut(s) 2288
SspMI CTAG 8 cut(s) 422, 683, 926, 1235, 1893, 1923, 2234, 2723
SstI GAGCTC 1 cut(s) 335
StuI AGGCCT 1 cut(s) 2133
StyD4I CCNGG 5 cut(s) 147, 303, 515, 1763, 2064
TaaI ACNGT 9 cut(s) 593, 710, 908, 1066, 1182, 1588, 2009, 2163, 2480
TatI WGTACW 1 cut(s) 1851
TauI GCSGC 5 cut(s) 102, 159, 676, 1109, 1694
TscAI CASTG 6 cut(s) 14, 715, 1593, 1666, 1867, 2584
TseFI GTSAC 3 cut(s) 238, 1199, 1582
TseI GCWGC 7 cut(s) 123, 214, 271, 1103, 1934, 1937, 2223
Tsp45I GTSAC 3 cut(s) 238, 1199, 1582
TspDTI ATGAA 8 cut(s) 270, 631, 719, 783, 1360, 1692, 2129, 2163
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 6 cut(s) 14, 715, 1593, 1666, 1867, 2584
Tth111I GACNNNGTC 1 cut(s) 1799
Van91I CCANNNNNTGG 1 cut(s) 2268
Vha464I CTTAAG 1 cut(s) 2042
VneI GTGCAC 2 cut(s) 1657, 2575
VpaK11BI GGWCC 6 cut(s) 151, 785, 2005, 2257, 2476, 2840
XapI RAATTY 5 cut(s) 17, 815, 1403, 2069, 2529
XbaI TCTAGA 2 cut(s) 1234, 2722
XceI RCATGY 4 cut(s) 952, 1460, 2191, 2586
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XhoI CTCGAG 3 cut(s) 94, 501, 1205
XmiI GTMKAC 1 cut(s) 745
XspI CTAG 8 cut(s) 422, 683, 926, 1235, 1893, 1923, 2234, 2723
Zsp2I ATGCAT 1 cut(s) 2122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.