FvH4_6g43130

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009775
Physical Location & Seq
Reverse (-)
33425335 .. 33427407
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43130.t1

Sequence Viewer

Length: 384 bp
ATGTTGGGTTTTAGCAGAAACGTTGTCGTCAAGAAGTCTTACAAGTCGGAAGTCCCTCTCGAGAAAAGGGAAGCTGAAGCTGCTCGCATCAGAGAGCAGCATCCTGATAGAGTACCGGTGATTGTTGAAAAGGCTGAAAAGAGTGATGTGCCTGATATTGAGAAGAACAAATACCTGGTGCCTGCCGATATTACTGTTGGGCAGTTAGTCTTTGTGATCCGGAAAAAGATCAAGCTTGATGCTGACCAGGCAATTTTCGTTTTCGTTAAGGATATTTTGCCACCTACAGCTGCTTTGATGTCTTCAATTTATGAGGAAAATAAGGACGAGGATGGTTTTCTTTATATGACATACAGTGGCGAGAGCACATTTGGGACGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

128

Amino Acids

14.38

Weight (kDa)

5.51

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 22 - 125 4.6e-48 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 178
AccIII TCCGGA 1 cut(s) 219
AclI AACGTT 1 cut(s) 21
AclWI GGATC 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 1 cut(s) 114
AgeI ACCGGT 1 cut(s) 115
AgsI TTSAA 2 cut(s) 128, 306
AjiI CACGTC 1 cut(s) 378
AjnI CCWGG 2 cut(s) 174, 246
AluBI AGCT 4 cut(s) 74, 80, 235, 290
AluI AGCT 4 cut(s) 74, 80, 235, 290
Alw21I GWGCWC 1 cut(s) 368
AlwI GGATC 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 59
Aor13HI TCCGGA 1 cut(s) 219
ApeKI GCWGC 3 cut(s) 80, 97, 290
AsiGI ACCGGT 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 59
BanI GGYRCC 1 cut(s) 178
BarI GAAGNNNNNNTAC 2 cut(s) 155, 187
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 3 cut(s) 67, 109, 277
BccI CCATC 1 cut(s) 326
BciT130I CCWGG 2 cut(s) 176, 248
BfmI CTRYAG 1 cut(s) 285
BisI GCNGC 3 cut(s) 81, 98, 291
BlsI GCNGC 3 cut(s) 82, 99, 292
Bme1390I CCNGG 2 cut(s) 176, 248
BmeT110I CYCGRG 1 cut(s) 59
BmgBI CACGTC 1 cut(s) 378
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 2 cut(s) 176, 248
BmsI GCATC 3 cut(s) 96, 109, 229
BpiI GAAGAC 1 cut(s) 294
BsaWI WCCGGW 2 cut(s) 115, 219
Bse118I RCCGGY 1 cut(s) 115
BseAI TCCGGA 1 cut(s) 219
BseBI CCWGG 2 cut(s) 176, 248
BseGI GGATG 2 cut(s) 100, 337
BseXI GCAGC 3 cut(s) 67, 109, 277
BshNI GGYRCC 1 cut(s) 178
BshTI ACCGGT 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 368
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 2 cut(s) 116, 220
BslFI GGGAC 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 38
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 368
Bsp13I TCCGGA 1 cut(s) 219
Bsp143I GATC 2 cut(s) 216, 228
BspEI TCCGGA 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 180
BspPI GGATC 1 cut(s) 211
BspT107I GGYRCC 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 115
BssAI RCCGGY 1 cut(s) 115
BssMI GATC 2 cut(s) 216, 228
Bst2UI CCWGG 2 cut(s) 176, 248
Bst4CI ACNGT 2 cut(s) 196, 356
BstC8I GCNNGC 2 cut(s) 85, 183
BstF5I GGATG 2 cut(s) 100, 337
BstKTI GATC 2 cut(s) 219, 231
BstMBI GATC 2 cut(s) 216, 228
BstMWI GCNNNNNNNGC 2 cut(s) 80, 248
BstNI CCWGG 2 cut(s) 176, 248
BstSCI CCNGG 2 cut(s) 174, 246
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 3 cut(s) 67, 109, 277
BstV2I GAAGAC 1 cut(s) 294
BtrI CACGTC 1 cut(s) 378
BtsCI GGATG 2 cut(s) 100, 337
BtsIMutI CAGTG 1 cut(s) 361
Cac8I GCNNGC 2 cut(s) 85, 183
Cfr10I RCCGGY 1 cut(s) 115
CsiI ACCWGGT 1 cut(s) 174
Csp6I GTAC 1 cut(s) 113
CspAI ACCGGT 1 cut(s) 115
CviJI RGCY 5 cut(s) 74, 80, 134, 235, 290
CviKI_1 RGCY 5 cut(s) 74, 80, 134, 235, 290
CviQI GTAC 1 cut(s) 113
DpnI GATC 2 cut(s) 218, 230
DpnII GATC 2 cut(s) 216, 228
Eco57I CTGAAG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 174, 246
FaiI YATR 4 cut(s) 312, 345, 347, 352
FaqI GGGAC 1 cut(s) 38
Fnu4HI GCNGC 3 cut(s) 81, 98, 291
FokI GGATG 2 cut(s) 87, 344
Fsp4HI GCNGC 3 cut(s) 81, 98, 291
GluI GCNGC 3 cut(s) 81, 98, 291
HapII CCGG 2 cut(s) 116, 220
HindIII AAGCTT 1 cut(s) 233
HpaII CCGG 2 cut(s) 116, 220
HphI GGTGA 1 cut(s) 130
Hpy188I TCNGA 2 cut(s) 49, 92
Hpy188III TCNNGA 5 cut(s) 31, 59, 61, 104, 220
HpyCH4III ACNGT 2 cut(s) 196, 356
HpyCH4IV ACGT 2 cut(s) 21, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 248
HpySE526I ACGT 2 cut(s) 21, 377
Kpn2I TCCGGA 1 cut(s) 219
Kzo9I GATC 2 cut(s) 216, 228
LpnPI CCDG 9 cut(s) 117, 129, 161, 165, 188, 195, 233, 233, 260
Lsp1109I GCAGC 3 cut(s) 67, 109, 277
LweI GCATC 3 cut(s) 96, 109, 229
MabI ACCWGGT 1 cut(s) 174
MaeII ACGT 2 cut(s) 21, 377
MalI GATC 2 cut(s) 218, 230
MboI GATC 2 cut(s) 216, 228
MboII GAAGA 2 cut(s) 175, 294
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 2 cut(s) 252, 306
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 3 cut(s) 66, 307, 322
MroI TCCGGA 1 cut(s) 219
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 2 cut(s) 116, 220
MspR9I CCNGG 2 cut(s) 176, 248
MvaI CCWGG 2 cut(s) 176, 248
MwoI GCNNNNNNNGC 2 cut(s) 80, 248
NdeII GATC 2 cut(s) 216, 228
NlaIV GGNNCC 1 cut(s) 180
PaeR7I CTCGAG 1 cut(s) 59
PinAI ACCGGT 1 cut(s) 115
PkrI GCNGC 3 cut(s) 82, 99, 292
Psp1406I AACGTT 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 174, 246
PspGI CCWGG 2 cut(s) 174, 246
PspN4I GGNNCC 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 3 cut(s) 81, 98, 291
Sau3AI GATC 2 cut(s) 216, 228
ScrFI CCNGG 2 cut(s) 176, 248
SduI GDGCHC 1 cut(s) 368
SetI ASST 8 cut(s) 24, 76, 82, 177, 237, 286, 292, 380
SexAI ACCWGGT 1 cut(s) 174
SfaNI GCATC 3 cut(s) 96, 109, 229
SfcI CTRYAG 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 2 cut(s) 252, 306
StyD4I CCNGG 2 cut(s) 174, 246
TaaI ACNGT 2 cut(s) 196, 356
TaiI ACGT 2 cut(s) 24, 380
TaqI TCGA 1 cut(s) 60
TasI AATT 2 cut(s) 252, 306
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 361
TseI GCWGC 3 cut(s) 80, 97, 290
TspRI CASTG 1 cut(s) 361
XhoI CTCGAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.