RLG00000021142

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009775
Physical Location & Seq
Reverse (-)
72299402 .. 72301022
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021142

Sequence Viewer

Length: 360 bp
ATGGCCAAGAAATCGTATAAGTTGGCGATCCCTCTCGAACAAAGGGAAGCAGAAGCTGCTCGCATCAGAGAGAATTATCCTGACAGGGTACCGGTGATTGTTGAAAGGGCTGAAAAGAGTGATGTGCCTGATATTGAGAAGAACAAATACCTGGTGCCTGCCGATATTACTGTTGGGCAGTTTGTCTTTGTGATCCGAAAAAAGATCAAGCTTGATCCCGACAAGGCTATTTTCATTTTTGTTAAGAATATTTTGCCACCCACAGCTGCTTTGATGTCTGCAATTTATGAGGAAAATAAGGATGAAGATGGTTTTCTTTATATGACATATAGTGGCGAGAGTACGTTTGGGACGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

120

Amino Acids

13.56

Weight (kDa)

5.56

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 14 - 117 5.5e-49 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 88
AccB1I GGYRCC 2 cut(s) 88, 154
AclWI GGATC 3 cut(s) 22, 187, 209
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 2 cut(s) 90, 343
AgeI ACCGGT 1 cut(s) 91
AgsI TTSAA 1 cut(s) 104
AjiI CACGTC 1 cut(s) 354
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 3 cut(s) 56, 211, 266
AluI AGCT 3 cut(s) 56, 211, 266
AlwI GGATC 3 cut(s) 22, 187, 209
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 56, 266
AsiGI ACCGGT 1 cut(s) 91
Asp718I GGTACC 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 106
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 88, 154
BarI GAAGNNNNNNTAC 2 cut(s) 131, 163
BbvI GCAGC 2 cut(s) 43, 253
BccI CCATC 1 cut(s) 302
BciT130I CCWGG 1 cut(s) 152
BisI GCNGC 2 cut(s) 57, 267
BlsI GCNGC 2 cut(s) 58, 268
Bme1390I CCNGG 1 cut(s) 152
BmgBI CACGTC 1 cut(s) 354
BmiI GGNNCC 2 cut(s) 90, 156
BmrFI CCNGG 1 cut(s) 152
BmsI GCATC 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 91
Bse118I RCCGGY 1 cut(s) 91
BseBI CCWGG 1 cut(s) 152
BseGI GGATG 1 cut(s) 307
BseXI GCAGC 2 cut(s) 43, 253
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 88, 154
BshTI ACCGGT 1 cut(s) 91
BsiSI CCGG 1 cut(s) 92
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 4 cut(s) 27, 192, 204, 214
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 2 cut(s) 90, 156
BspPI GGATC 3 cut(s) 22, 187, 209
BspT107I GGYRCC 2 cut(s) 88, 154
BsrFI RCCGGY 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 91
BssMI GATC 4 cut(s) 27, 192, 204, 214
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 172
BstAPI GCANNNNNTGC 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 61, 159
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 4 cut(s) 30, 195, 207, 217
BstMBI GATC 4 cut(s) 27, 192, 204, 214
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 43, 253
BsuRI GGCC 1 cut(s) 5
BtrI CACGTC 1 cut(s) 354
BtsCI GGATG 1 cut(s) 307
Cac8I GCNNGC 2 cut(s) 61, 159
CaiI CAGNNNCTG 1 cut(s) 56
Cfr10I RCCGGY 1 cut(s) 91
CsiI ACCWGGT 1 cut(s) 150
Csp6I GTAC 2 cut(s) 89, 342
CspAI ACCGGT 1 cut(s) 91
CviJI RGCY 6 cut(s) 5, 56, 110, 211, 227, 266
CviKI_1 RGCY 6 cut(s) 5, 56, 110, 211, 227, 266
CviQI GTAC 2 cut(s) 89, 342
DpnI GATC 4 cut(s) 29, 194, 206, 216
DpnII GATC 4 cut(s) 27, 192, 204, 214
EaeI YGGCCR 1 cut(s) 3
EcoRII CCWGG 1 cut(s) 150
FaiI YATR 6 cut(s) 18, 288, 321, 323, 328, 330
Fnu4HI GCNGC 2 cut(s) 57, 267
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 2 cut(s) 57, 267
GluI GCNGC 2 cut(s) 57, 267
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 209
HpaII CCGG 1 cut(s) 92
HphI GGTGA 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 68, 197
Hpy188III TCNNGA 3 cut(s) 35, 80, 218
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 2 cut(s) 344, 353
HpyCH4V TGCA 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpySE526I ACGT 2 cut(s) 344, 353
KpnI GGTACC 1 cut(s) 92
Kzo9I GATC 4 cut(s) 27, 192, 204, 214
LpnPI CCDG 7 cut(s) 70, 93, 105, 137, 141, 164, 171
Lsp1109I GCAGC 2 cut(s) 43, 253
LweI GCATC 1 cut(s) 72
MabI ACCWGGT 1 cut(s) 150
MaeII ACGT 2 cut(s) 344, 353
MalI GATC 4 cut(s) 29, 194, 206, 216
MboI GATC 4 cut(s) 27, 192, 204, 214
MboII GAAGA 2 cut(s) 151, 317
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 73, 282
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 2 cut(s) 42, 283
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 243
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 56
NdeII GATC 4 cut(s) 27, 192, 204, 214
NlaIV GGNNCC 2 cut(s) 90, 156
PcsI WCGNNNNNNNCGW 1 cut(s) 350
PinAI ACCGGT 1 cut(s) 91
PkrI GCNGC 2 cut(s) 58, 268
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 2 cut(s) 90, 156
PstNI CAGNNNCTG 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 2 cut(s) 90, 343
RsaNI GTAC 2 cut(s) 89, 342
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 2 cut(s) 57, 267
Sau3AI GATC 4 cut(s) 27, 192, 204, 214
ScrFI CCNGG 1 cut(s) 152
SetI ASST 6 cut(s) 58, 153, 213, 268, 347, 356
SexAI ACCWGGT 1 cut(s) 150
SfaNI GCATC 1 cut(s) 72
Sse9I AATT 2 cut(s) 73, 282
SspI AATATT 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 2 cut(s) 347, 356
TaqI TCGA 1 cut(s) 36
TasI AATT 2 cut(s) 73, 282
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TseI GCWGC 2 cut(s) 56, 266
TspDTI ATGAA 2 cut(s) 223, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.