Rorug02G0474600

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009775
Physical Location & Seq
Reverse (-)
60591237 .. 60601221
9985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0474600.1

Sequence Viewer

Length: 888 bp
ATGTCTTCTTCTAAGGTTTCCATGGTGCTTCTTCTTTCTTTTTTCATAGCTTCTATTCTAGCCATGACTGCCTTAGCTGGCAATTTCTTTCAAGAGTTTGACATCACTTTTGGCGATCAACGCGTTAAAATACTCGAAGGAGGAAAATTTCTGATGCTCAACCTCGACCAGTATTCTGGCTCTGGTTTCAAATCCAAGAACGAGTACTTATTCGGAAGGATCGACATGCAAATCAAACTTGTACCTGGAAACTCAGCTGGCACTGTCACTACATACTATTTATCTTCTGAAGGTCCAACTCATGATGAAATCGACTTCGAGTTTTTGGGCAACTCATCTGGAGATCCCTACACTCTCCACACCAATGTATTCAGTCAGGGCAAAGGGGATAGAGAACAACAATTCCATCTTTGGTTTGATCCCACAAAGGCCTTCCACACCTACTCATTTGTCTGGAACTCCCAACGCATTATATTCTTTGTCGACAACATTCCAATCCGAGTGTTCAACAACATGGAATCATTTGGTGTTCCATTCCCCAAAAACCAGCCAATGAGGATTTACTCGAGTCTGTGGAATGCCGACGACTGGGCTACACAAGGCGGGCGTGTGAAGACTGACTGGACGCAAGCTCCTTTCACTGCTTCGTACCGAAACTTCAAGGCCAATGCTTGTGTTGCTGGATCCTCGTCCTCCTCCTGCGGTGTCGCCACCGTTGCATCGACTGATAAGACACAGCAGAGTGCATGGCAGACTCAAACGCTTGATGCTGCAGGGCGAAATAGGCTTCGATGGGTGCAACAAAAGTTCATGGTGTACAACTACTGCTCTGACCTCAAAAGATTCCCCCAAGGCCTTCCGGTCGAATGCAAGCGATCAAGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

295

Amino Acids

33.42

Weight (kDa)

8.8

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 31 - 209 2.6e-65 Glycosyl hydrolases family 16
XET_C PF06955 246 - 290 2.9e-19 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 483
AccII CGCG 1 cut(s) 123
AciI CCGC 2 cut(s) 603, 702
AclWI GGATC 5 cut(s) 227, 338, 413, 678, 691
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 4 cut(s) 206, 243, 650, 818
AfiI CCNNNNNNNGG 1 cut(s) 588
AflIII ACRYGT 1 cut(s) 121
AgsI TTSAA 4 cut(s) 92, 190, 508, 661
AjnI CCWGG 1 cut(s) 244
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
AluBI AGCT 4 cut(s) 50, 77, 257, 632
AluI AGCT 4 cut(s) 50, 77, 257, 632
AlwI GGATC 5 cut(s) 227, 338, 413, 678, 691
Ama87I CYCGRG 1 cut(s) 565
AoxI GGCC 3 cut(s) 429, 663, 853
ApeKI GCWGC 1 cut(s) 770
ApoI RAATTY 1 cut(s) 146
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
AspS9I GGNCC 1 cut(s) 293
AvaI CYCGRG 1 cut(s) 565
AvaII GGWCC 1 cut(s) 293
BamHI GGATCC 1 cut(s) 683
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BbsI GAAGAC 1 cut(s) 620
BbvI GCAGC 1 cut(s) 757
BccI CCATC 2 cut(s) 414, 786
BciT130I CCWGG 1 cut(s) 246
BfaI CTAG 2 cut(s) 59, 886
BfmI CTRYAG 1 cut(s) 771
BisI GCNGC 1 cut(s) 771
BlsI GCNGC 1 cut(s) 772
BmcAI AGTACT 1 cut(s) 206
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 1 cut(s) 685
BmrFI CCNGG 1 cut(s) 246
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 3 cut(s) 144, 728, 757
BmuI ACTGGG 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 620
BpmI CTGGAG 1 cut(s) 360
Bpu10I CCTNAGC 1 cut(s) 73
BsaJI CCNNGG 2 cut(s) 21, 850
BsaWI WCCGGW 1 cut(s) 859
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse1I ACTGG 3 cut(s) 169, 593, 626
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 2 cut(s) 21, 850
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 3 cut(s) 169, 593, 626
BseRI GAGGAG 1 cut(s) 685
BseXI GCAGC 1 cut(s) 757
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 864
BshFI GGCC 3 cut(s) 431, 665, 855
BsiEI CGRYCG 1 cut(s) 864
BsiHKCI CYCGRG 1 cut(s) 565
BsiSI CCGG 1 cut(s) 860
BslI CCNNNNNNNGG 1 cut(s) 588
BsmI GAATGC 2 cut(s) 583, 872
BsnI GGCC 3 cut(s) 431, 665, 855
BsoBI CYCGRG 1 cut(s) 565
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 6 cut(s) 115, 219, 343, 418, 683, 875
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 2 cut(s) 603, 702
BspANI GGCC 3 cut(s) 431, 665, 855
BspCNI CTCAG 1 cut(s) 266
BspFNI CGCG 1 cut(s) 123
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 685
BspMAI CTGCAG 1 cut(s) 775
BspPI GGATC 5 cut(s) 227, 338, 413, 678, 691
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 3 cut(s) 169, 593, 626
BssECI CCNNGG 2 cut(s) 21, 850
BssMI GATC 6 cut(s) 115, 219, 343, 418, 683, 875
BssT1I CCWWGG 2 cut(s) 21, 850
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 265, 715
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 5 cut(s) 79, 259, 605, 630, 872
BstDEI CTNAG 3 cut(s) 12, 73, 253
BstDSI CCRYGG 1 cut(s) 21
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 6 cut(s) 118, 222, 346, 421, 686, 878
BstMBI GATC 6 cut(s) 115, 219, 343, 418, 683, 875
BstMCI CGRYCG 1 cut(s) 864
BstMWI GCNNNNNNNGC 5 cut(s) 68, 120, 677, 716, 784
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 771
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 757
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 2 cut(s) 343, 683
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 2 cut(s) 343, 683
BsuRI GGCC 3 cut(s) 431, 665, 855
BtgI CCRYGG 1 cut(s) 21
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 2 cut(s) 261, 639
Cac8I GCNNGC 5 cut(s) 79, 259, 605, 630, 872
CciI TCATGA 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 1 cut(s) 634
Csp6I GTAC 4 cut(s) 205, 242, 649, 817
CviAII CATG 7 cut(s) 22, 64, 226, 302, 514, 747, 811
CviQI GTAC 4 cut(s) 205, 242, 649, 817
DdeI CTNAG 3 cut(s) 12, 73, 253
DpnI GATC 6 cut(s) 117, 221, 345, 420, 685, 877
DpnII GATC 6 cut(s) 115, 219, 343, 418, 683, 875
Eco130I CCWWGG 2 cut(s) 21, 850
Eco147I AGGCCT 2 cut(s) 431, 855
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 21, 850
ErhI CCWWGG 2 cut(s) 21, 850
FaeI CATG 7 cut(s) 25, 67, 229, 305, 517, 750, 814
FatI CATG 7 cut(s) 21, 63, 225, 301, 513, 746, 810
FauI CCCGC 1 cut(s) 596
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 1 cut(s) 771
Fsp4HI GCNGC 1 cut(s) 771
FspBI CTAG 2 cut(s) 59, 886
GluI GCNGC 1 cut(s) 771
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 3 cut(s) 431, 665, 855
HapII CCGG 1 cut(s) 860
HgaI GACGC 1 cut(s) 634
Hin1II CATG 7 cut(s) 25, 67, 229, 305, 517, 750, 814
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 4 cut(s) 518, 568, 754, 843
HpaII CCGG 1 cut(s) 860
Hpy166II GTNNAC 2 cut(s) 484, 817
Hpy188I TCNGA 5 cut(s) 153, 215, 289, 500, 832
Hpy188III TCNNGA 4 cut(s) 92, 302, 339, 454
Hpy8I GTNNAC 2 cut(s) 484, 817
Hpy99I CGWCG 1 cut(s) 587
HpyAV CCTTC 5 cut(s) 131, 210, 284, 442, 866
HpyCH4III ACNGT 2 cut(s) 265, 715
HpyCH4V TGCA 6 cut(s) 229, 719, 746, 773, 799, 870
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 120, 677, 716, 784
HpyF3I CTNAG 3 cut(s) 12, 73, 253
Hsp92II CATG 7 cut(s) 25, 67, 229, 305, 517, 750, 814
Kzo9I GATC 6 cut(s) 115, 219, 343, 418, 683, 875
LmnI GCTCC 1 cut(s) 637
Lsp1109I GCAGC 1 cut(s) 757
LweI GCATC 3 cut(s) 144, 728, 757
MaeI CTAG 2 cut(s) 59, 886
MaeIII GTNAC 1 cut(s) 265
MalI GATC 6 cut(s) 117, 221, 345, 420, 685, 877
MboI GATC 6 cut(s) 115, 219, 343, 418, 683, 875
MboII GAAGA 3 cut(s) 23, 276, 625
MflI RGATCY 2 cut(s) 343, 683
MluCI AATT 3 cut(s) 82, 146, 401
MluI ACGCGT 1 cut(s) 121
MlyI GAGTC 2 cut(s) 577, 748
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 7 cut(s) 134, 173, 549, 697, 703, 706, 845
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 1 cut(s) 860
MspR9I CCNGG 1 cut(s) 246
Mva1269I GAATGC 2 cut(s) 583, 872
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 5 cut(s) 68, 120, 677, 716, 784
NcoI CCATGG 1 cut(s) 21
NdeII GATC 6 cut(s) 115, 219, 343, 418, 683, 875
NlaIII CATG 7 cut(s) 25, 67, 229, 305, 517, 750, 814
NlaIV GGNNCC 1 cut(s) 685
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 565
PagI TCATGA 1 cut(s) 301
PceI AGGCCT 2 cut(s) 431, 855
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 2 cut(s) 583, 872
PfeI GAWTC 2 cut(s) 518, 843
PkrI GCNGC 1 cut(s) 772
PleI GAGTC 2 cut(s) 576, 748
PpsI GAGTC 2 cut(s) 576, 748
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 1 cut(s) 293
PspXI VCTCGAGB 1 cut(s) 565
PstI CTGCAG 1 cut(s) 775
PsuI RGATCY 2 cut(s) 343, 683
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 4 cut(s) 206, 243, 650, 818
RsaNI GTAC 4 cut(s) 205, 242, 649, 817
RseI CAYNNNNRTG 1 cut(s) 363
SalI GTCGAC 1 cut(s) 482
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 1 cut(s) 771
Sau3AI GATC 6 cut(s) 115, 219, 343, 418, 683, 875
Sau96I GGNCC 1 cut(s) 293
ScaI AGTACT 1 cut(s) 206
SchI GAGTC 2 cut(s) 577, 748
ScrFI CCNGG 1 cut(s) 246
SfaNI GCATC 3 cut(s) 144, 728, 757
SfcI CTRYAG 1 cut(s) 771
Sfr274I CTCGAG 1 cut(s) 565
SinI GGWCC 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 565
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
Sse9I AATT 3 cut(s) 82, 146, 401
SseBI AGGCCT 2 cut(s) 431, 855
SsiI CCGC 2 cut(s) 603, 702
SspMI CTAG 2 cut(s) 59, 886
StuI AGGCCT 2 cut(s) 431, 855
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 2 cut(s) 21, 850
TaaI ACNGT 2 cut(s) 265, 715
TasI AATT 3 cut(s) 82, 146, 401
TatI WGTACW 2 cut(s) 204, 816
TfiI GAWTC 2 cut(s) 518, 843
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 2 cut(s) 268, 646
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 1 cut(s) 770
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 3 cut(s) 34, 321, 799
TspRI CASTG 2 cut(s) 268, 646
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 146
XceI RCATGY 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 565
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 2 cut(s) 59, 886
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.