Rh5AG271200

Belongs to the ATG8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009775
Physical Location & Seq
Forward (+)
35952031 .. 35955193
3163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG271200.1

Sequence Viewer

Length: 360 bp
ATGGCCAAAAGCTCCTTCAAGCTGGAACACCCTCTGGAGAGGAGGCAGGCAGAAGCTGCTCGTATCAGGGAGAAGTATCCTGACAGAATTCCGGTTATTGTGGAGAAGGCTGAAAGGAGTGACATACCAGACATTGACAAGAAGAAGTACCTGGTTCCTGCTGATCTGACTGTTGGGCAGTTTGTTTATGTGGTCCGGAAGAGGATAAAACTTAGTGCTGAGAAAGCCATATTTATATTTGTCAAGAACATTTTGCCACCTACTGCTGCCATGATGTCGGCAATCTATGAGGAAAACAAAGACGAGGATGGTTTCCTTTATATGACTTACAGTGGAGAAAATACATTTGGATCCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

119

Amino Acids

13.72

Weight (kDa)

8.78

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 14 - 117 8.1e-52 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 195
AclWI GGATC 2 cut(s) 345, 358
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 87
AfaI GTAC 1 cut(s) 149
AgsI TTSAA 1 cut(s) 19
AjnI CCWGG 1 cut(s) 150
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 330
AluBI AGCT 3 cut(s) 12, 22, 56
AluI AGCT 3 cut(s) 12, 22, 56
AlwI GGATC 2 cut(s) 345, 358
AlwNI CAGNNNCTG 1 cut(s) 56
Aor13HI TCCGGA 1 cut(s) 195
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 56, 266
ApoI RAATTY 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 193
AvaII GGWCC 1 cut(s) 193
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 350
BbvI GCAGC 2 cut(s) 43, 253
BccI CCATC 1 cut(s) 302
BciT130I CCWGG 1 cut(s) 152
BciVI GTATCC 1 cut(s) 87
BfuI GTATCC 1 cut(s) 87
BisI GCNGC 2 cut(s) 57, 267
BlsI GCNGC 2 cut(s) 58, 268
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 2 cut(s) 156, 352
BmrFI CCNGG 1 cut(s) 152
BpmI CTGGAG 1 cut(s) 56
BsaWI WCCGGW 2 cut(s) 91, 195
BseAI TCCGGA 1 cut(s) 195
BseBI CCWGG 1 cut(s) 152
BseGI GGATG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 210
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 2 cut(s) 43, 253
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 2 cut(s) 92, 196
BsnI GGCC 1 cut(s) 5
Bsp13I TCCGGA 1 cut(s) 195
Bsp143I GATC 2 cut(s) 163, 350
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 211
BspEI TCCGGA 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 156, 352
BspPI GGATC 2 cut(s) 345, 358
BssMI GATC 2 cut(s) 163, 350
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 2 cut(s) 172, 332
Bst6I CTCTTC 1 cut(s) 194
BstAPI GCANNNNNTGC 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 2 cut(s) 212, 219
BstF5I GGATG 1 cut(s) 313
BstKTI GATC 2 cut(s) 166, 353
BstMBI GATC 2 cut(s) 163, 350
BstMWI GCNNNNNNNGC 2 cut(s) 56, 224
BstNI CCWGG 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 43, 253
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
BsuI GTATCC 1 cut(s) 87
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 48
CaiI CAGNNNCTG 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 193
CsiI ACCWGGT 1 cut(s) 150
Csp6I GTAC 1 cut(s) 148
CviAII CATG 1 cut(s) 271
CviJI RGCY 6 cut(s) 5, 12, 22, 56, 110, 227
CviKI_1 RGCY 6 cut(s) 5, 12, 22, 56, 110, 227
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 2 cut(s) 212, 219
DpnI GATC 2 cut(s) 165, 352
DpnII GATC 2 cut(s) 163, 350
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 193
EcoRI GAATTC 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 150
FaeI CATG 1 cut(s) 274
FaiI YATR 8 cut(s) 125, 189, 230, 236, 272, 288, 321, 323
FatI CATG 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 57, 267
FokI GGATG 1 cut(s) 320
Fsp4HI GCNGC 2 cut(s) 57, 267
GluI GCNGC 2 cut(s) 57, 267
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 92, 196
Hin1II CATG 1 cut(s) 274
HpaII CCGG 2 cut(s) 92, 196
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 4 cut(s) 35, 80, 196, 244
HpyAV CCTTC 2 cut(s) 25, 100
HpyCH4III ACNGT 2 cut(s) 172, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 224
HpyF3I CTNAG 2 cut(s) 212, 219
Hsp92II CATG 1 cut(s) 274
Kpn2I TCCGGA 1 cut(s) 195
Kzo9I GATC 2 cut(s) 163, 350
LmnI GCTCC 1 cut(s) 17
Lsp1109I GCAGC 2 cut(s) 43, 253
MabI ACCWGGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 119
MalI GATC 2 cut(s) 165, 352
MboI GATC 2 cut(s) 163, 350
MboII GAAGA 2 cut(s) 154, 211
MflI RGATCY 1 cut(s) 350
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 87
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 33, 36, 42, 195, 283, 298
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 195
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 358
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 92, 196
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 2 cut(s) 56, 224
NdeII GATC 2 cut(s) 163, 350
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 2 cut(s) 156, 352
NmuCI GTSAC 1 cut(s) 119
PkrI GCNGC 2 cut(s) 58, 268
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 2 cut(s) 156, 352
PspPI GGNCC 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 350
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SaqAI TTAA 1 cut(s) 358
SatI GCNGC 2 cut(s) 57, 267
Sau3AI GATC 2 cut(s) 163, 350
Sau96I GGNCC 1 cut(s) 193
ScrFI CCNGG 1 cut(s) 152
SetI ASST 5 cut(s) 14, 24, 58, 153, 262
SexAI ACCWGGT 1 cut(s) 150
SinI GGWCC 1 cut(s) 193
Sse9I AATT 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 172, 332
TasI AATT 1 cut(s) 87
Tru1I TTAA 1 cut(s) 358
Tru9I TTAA 1 cut(s) 358
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 2 cut(s) 56, 266
Tsp45I GTSAC 1 cut(s) 119
TspRI CASTG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.