FvH4_6g44560

vacuolar protein sorting-associated protein 18 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016149
Physical Location & Seq
Reverse (-)
34315250 .. 34325462
10213 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44560.t5

Sequence Viewer

Length: 2964 bp
ATGGATTCGGCGAGGCAGGTTTTCACGGTGGACCTTCTAGAAAGGTACGCCGCGAAAGGCCGCGGAGTCATCACGTGTATGGCTGCCGGAAATGACGTCATTGTGCTCGGAACTAGTAAAGGTTGGATAATCCGGCATGATTTCGGGGTCGGAGATTCGTTCGATTTTGATCTCTCTACGGGTCGTCCGGGGGAGCATTCGATTCATAGAGTTTTTGTTGATCCTGGAGGGAGCCACTGTATCGCCTGCATTGTTGGTACCGGTGGTGCTGATACTTTCTATATGCATGCGAAATGGAGCAAGCCGCGTGTTTTGACCAAGTTGAAAGGCCTTGTTGTGAATGCTGTTGCCTGGAACAGACAGCAGATTACAGAAGTTTCGACAAAGGAAGTTATTCTTGGTACAGACAACGGACAACTTTATGAGATTGCTGTGGATGAGAAGGATAAGAAGGAGAAGTATGTGAAGTTTCTTTATGAACTAATTGAACTTCCTGAGGCCTTCATGAGTTTGCAGATGGAAACTGCCACGATTCTGAACGGAACTAGATACTATGTGATGGCTGTTACTCCTACACGACTCTATTCTTATACAGGAATTGGATTGTTGGATGCAATTTTTGCTAGTTACTTGGAGCATCCGGTGCGTTTCATGGAGCTTCCCGGTGAAATACCTAACAGTGAATTGCATTTCTATATCAAGCAAAGACGAGCAGTACATTTTGCATGGCTTTCTGGAGCTGGAATCTATAATGGCGGTTTAAATTTTGGTGCTCAACATAGTTCTTCAACTGGAGATGAGAATTTTGTGGAGAACAAGGCTCTTCTGGCATATTCAAAATTGTCTGAGAGTTCTGAAGTTGTGATGCCCACTTCTATGGCCGTTTCAGAATTTCATTTCCTGCTTCTTATCGGGAATAAGGTTAAGGTTGTAAATAGAATAAGTGAGCAAATTATAGAGGAACTTCAGTTTGATCAAACATCCGAATCAGCTTCAAGGGGTATCATTGGATTATGTAGTGATGCCACAGCTGGACTGTTCTATGCTTATGACCAGAACTCTGTATTTCAGGTGTCTGTTAATGATGAAGGCCGAGATATGTGGAAAGTTTATCTGGACATGAAAGAGTATGCTGCTGCTTTGGCAAATTGTCGCGACCCACTTCAAAGGGACCAAGTATATTTAGTACAGGCTGAGGCTGCATTTAACTCAAAAGATTATCTCAGAGCAGCATCTTTCTATGCAAAGATAAACTACATATTATCATTCGAGGAGATCACTCTGAAGTTTATCACTGTCAATGAACAGGATGCTTTGAGAACCTTTTTGTTGCGGAAACTCGACTGTCTTGCGAAGGATGACAAGTGCCAAATAACCATGATTTCAACGTGGACAACTGAATTGTACTTAGATAAGATAAATCGCCTACTGTTGGAAGATGACACTGCATTGGAAAATCGGAATTCAGAGTATCAATCTATTATTAAAGAGTTTCGTGCATTTCTGAGTGACTCCAAGGATGTATTGGATGAAGCAACCACAATGAGACTATTAGAAAGTTATGGTCGAGTTGAAGAGTTGGTATTTTTTGCTAGTCTGAAAGAACAATATGAAATTGTAGTGCACCATTATATTCAGCAAGGAGAAGCGAAGAAGGCACTGGAGGTTCTTCAAAAACCTTCTGTTCCTATTGATCTTCAGTATAAGTTTGCTCCTGACCTAATTATGCTTGACGCATATGAAGCTGTGGAGTCATGGATGGCGACAAACAACCTCAACCCTAGGAAACTCATTCCGGCAATGATGCGCTATTCGAGTGAACCGCATGCAAAAAATGAGACGCATGAAGTCATCAAATACTTGGAATACTGTGTTCACCGTTTGCACAATGAGGACCCTGGAGTTCACAACTTGCTTTTATCACTATATGCCAAGCAGGAAGATGATAGTGCACTTTTGCGCTTCCTACAATTTAAATTTGGAAAAGGACGGGAAAGTGGCCCTGAATTCTTCTATGATCCCAAGTATGCTTTACGCCTTTGCCTTAAAGAAAAAAGAATGCGTGCCTGTGTTCATATATACAGTATGATGTCAATGCATGAAGAAGCAGTTGCTCTCGCCCTCCAGGTTGATCCTGAGCTTGCTATGGCTGAAGCTGACAAGGTTGAAGATGATGAGGACTTGAGAAAGAAGCTCTGGCTCATGGTTGCGAAGCATGTAATAGAACAGGAGAAGGGGGCTAAGAGGGAGAACATACGGAAGGCAATAGCATTTCTTAAAGAAACAGATGGCCTTTTAAAGATAGAGGATATATTACCATTCTTTCCAGACTTTGCCCTAATTGATGACTTTAAGGAGGCAATTTGCTCATCATTAGAGGATTACAATAATCAAATTGAACAACTCAAACAAGAGATGAATGATGCGACACATGGTGCTGACAACATCAGAAATGACATTAGTGCCCTTGCTCAAAGATATGCTGTGATTGATCGTGATGAAGAATGTGGGGTGTGTCGGCGTAAAATATTAACTGTCGGTAGGGAGTATCAGTTGAGTAGGGGCTATTCAACAGTAGGGCAAATGGCCCCCTTTTATGTCTTTCCTTGTGGACATGCCTTCCATGCACAATGCCTGATTGCCCATGTTACACGTAGCACTAATGAAGCTCAAGCAGAGTATATTCTGGATCTGCAGAAGCAACTCACTCTATTGGATGGGGAATCTAGGAAGGATTCAAATGGTCCCCTGACTGACGAAACTTTAACAAGCATGGCTCCTGTAGACAAGCTCCGATCACAGTTGGATGATGCTGTGGCGAGCGAGTGCCCATTTTGTGGGGACTTAATGATCCGCGAGATCTCTCTGCCTTTCATTCTTCCCGAGGAACAATATTCAAGTACTTCATGGGATATACAATCGCGTAATCTTGGAAACCAGAGGAGTCTTTCTTTATCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006904 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007275 GO:0007610 GO:0007632 GO:0007634 GO:0008104 GO:0008150 GO:0008333 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009653 GO:0009705 GO:0009790 GO:0009987 GO:0010015 GO:0010256 GO:0010876 GO:0012505 GO:0015711 GO:0015718 GO:0015721 GO:0015849 GO:0015850 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0021700 GO:0022406 GO:0022622 GO:0030154 GO:0030674 GO:0030897 GO:0030903 GO:0031090 GO:0031338 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032879 GO:0032940 GO:0032991 GO:0033036 GO:0033043 GO:0033059 GO:0033263 GO:0035542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043473 GO:0043476 GO:0043482 GO:0043485 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045176 GO:0046903 GO:0046907 GO:0046942 GO:0048066 GO:0048069 GO:0048278 GO:0048364 GO:0048468 GO:0048513 GO:0048568 GO:0048731 GO:0048757 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0050931 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0051716 GO:0060034 GO:0060035 GO:0060036 GO:0060090 GO:0060627 GO:0065007 GO:0071702 GO:0071840 GO:0080171 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099402 GO:0140029 GO:0140056 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin Pep3_Vps18

Protein Analysis

988

Amino Acids

112.12

Weight (kDa)

5.17

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin PF00637 152 - 285 3.1e-08 Region in Clathrin and VPS
VPS18_RING_C PF26148 406 - 493 7.7e-07 VPS18 RING C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12470
fragaria_vesca FvH4_6g44560 FvH4_6g44560
malus_domestica MD14G1065300.v1.1 MD17G1084700.v1.1
prunus_persica Prupe.3G231000_v2.0.a1
pyrus_communis pycom17g08190
rosa_chinensis RchiOBHm_Chr2g0161771
rosa_laevigata RLG00000021298
rosa_multiflora Rmu_sc0003662.1_g000015
rosa_roxburghii Rroxscaffold_2G00088620
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2BG571500 Rh2DG580400
rosa_wichuraiana Rw2G046160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 7
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 257
AccB7I CCANNNNNTGG 1 cut(s) 2837
AccI GTMKAC 1 cut(s) 2784
AccII CGCG 6 cut(s) 53, 63, 307, 1155, 2856, 2923
AciI CCGC 8 cut(s) 51, 61, 63, 305, 756, 1333, 1823, 2854
AclWI GGATC 5 cut(s) 215, 2012, 2126, 2697, 2845
AcoI YGGCCR 1 cut(s) 879
AcsI RAATTY 6 cut(s) 763, 802, 890, 1462, 1976, 2006
AcuI CTGAAG 5 cut(s) 876, 950, 1304, 1682, 2172
AcvI CACGTG 1 cut(s) 75
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 2435
AfaI GTAC 7 cut(s) 47, 259, 403, 717, 1188, 1406, 2902
AfiI CCNNNNNNNGG 2 cut(s) 1432, 2837
AflIII ACRYGT 2 cut(s) 74, 2651
AgeI ACCGGT 1 cut(s) 260
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 4 cut(s) 223, 350, 1897, 2124
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
Alw21I GWGCWC 4 cut(s) 108, 775, 1626, 1954
Alw26I GTCTC 2 cut(s) 1540, 1832
Alw44I GTGCAC 2 cut(s) 1622, 1950
AlwI GGATC 5 cut(s) 215, 2012, 2126, 2697, 2845
Ama87I CYCGRG 1 cut(s) 2882
AoxI GGCC 8 cut(s) 58, 328, 498, 879, 1090, 1999, 2290, 2586
ApaLI GTGCAC 2 cut(s) 1622, 1950
ApeKI GCWGC 5 cut(s) 83, 1133, 1136, 1199, 1229
ApoI RAATTY 6 cut(s) 763, 802, 890, 1462, 1976, 2006
AsiGI ACCGGT 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 257
AspA2I CCTAGG 1 cut(s) 1781
AspLEI GCGC 2 cut(s) 1809, 1962
AspS9I GGNCC 6 cut(s) 31, 1171, 1894, 2000, 2587, 2744
AsuC2I CCSGG 2 cut(s) 189, 663
AsuHPI GGTGA 2 cut(s) 677, 1868
AvaI CYCGRG 1 cut(s) 2882
AvaII GGWCC 4 cut(s) 31, 1171, 1894, 2744
AvrII CCTAGG 1 cut(s) 1781
AxyI CCTNAGG 1 cut(s) 495
BaeGI GKGCMC 4 cut(s) 1626, 1954, 2467, 2831
BanI GGYRCC 1 cut(s) 257
BbrPI CACGTG 1 cut(s) 75
Bbv12I GWGCWC 4 cut(s) 108, 775, 1626, 1954
BbvCI CCTCAGC 1 cut(s) 1194
BbvI GCAGC 5 cut(s) 70, 1120, 1123, 1186, 1241
BccI CCATC 5 cut(s) 511, 553, 1753, 2282, 2711
BceAI ACGGC 1 cut(s) 866
BcgI CGANNNNNNTGC 2 cut(s) 1331, 1365
BciT130I CCWGG 4 cut(s) 225, 352, 1899, 2126
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 2 cut(s) 189, 663
BcoDI GTCTC 2 cut(s) 1540, 1832
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 7 cut(s) 38, 114, 546, 624, 1593, 1782, 2727
BfmI CTRYAG 2 cut(s) 2693, 2781
BfuAI ACCTGC 1 cut(s) 7
BglII AGATCT 1 cut(s) 2859
BisI GCNGC 8 cut(s) 51, 61, 84, 305, 1134, 1137, 1200, 1230
BlnI CCTAGG 1 cut(s) 1781
BlsI GCNGC 8 cut(s) 52, 62, 85, 306, 1135, 1138, 1201, 1231
BmcAI AGTACT 1 cut(s) 2902
Bme1390I CCNGG 6 cut(s) 189, 225, 352, 663, 1899, 2126
Bme18I GGWCC 4 cut(s) 31, 1171, 1894, 2744
BmeT110I CYCGRG 1 cut(s) 2882
BmgT120I GGNCC 6 cut(s) 31, 1171, 1894, 2000, 2587, 2744
BmiI GGNNCC 7 cut(s) 233, 259, 1172, 1896, 2589, 2746, 2778
BmrFI CCNGG 6 cut(s) 189, 225, 352, 663, 1899, 2126
BmsI GCATC 9 cut(s) 601, 646, 855, 1012, 1241, 1300, 1794, 2413, 2800
BpmI CTGGAG 6 cut(s) 246, 756, 813, 1682, 1920, 2108
Bpu10I CCTNAGC 2 cut(s) 1194, 2136
BpuEI CTTGAG 2 cut(s) 2203, 2655
BpuMI CCSGG 2 cut(s) 189, 663
BsaAI YACGTR 2 cut(s) 75, 2654
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 6 cut(s) 61, 188, 1515, 1781, 1897, 2883
BsaWI WCCGGW 2 cut(s) 260, 640
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 2537, 2567
Bsc4I CCNNNNNNNGG 2 cut(s) 1432, 2837
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 796, 1665
Bse21I CCTNAGG 1 cut(s) 495
Bse3DI GCAATG 1 cut(s) 1806
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 4 cut(s) 225, 352, 1899, 2126
BseDI CCNNGG 6 cut(s) 61, 188, 1515, 1781, 1897, 2883
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 1432, 2837
BseMI GCAATG 1 cut(s) 1806
BseMII CTCAG 6 cut(s) 486, 837, 1185, 1237, 1496, 2127
BseNI ACTGG 2 cut(s) 796, 1665
BseRI GAGGAG 2 cut(s) 1286, 2956
BseSI GKGCMC 4 cut(s) 1626, 1954, 2467, 2831
BseXI GCAGC 5 cut(s) 70, 1120, 1123, 1186, 1241
Bsh1236I CGCG 6 cut(s) 53, 63, 307, 1155, 2856, 2923
BshFI GGCC 8 cut(s) 60, 330, 500, 881, 1092, 2001, 2292, 2588
BshNI GGYRCC 1 cut(s) 257
BshTI ACCGGT 1 cut(s) 260
BsiHKAI GWGCWC 4 cut(s) 108, 775, 1626, 1954
BsiHKCI CYCGRG 1 cut(s) 2882
BsiSI CCGG 7 cut(s) 87, 133, 188, 261, 641, 663, 1796
BslFI GGGAC 3 cut(s) 1184, 2730, 2855
BslI CCNNNNNNNGG 2 cut(s) 1432, 2837
BsmAI GTCTC 2 cut(s) 1540, 1832
BsmBI CGTCTC 1 cut(s) 1832
BsmFI GGGAC 3 cut(s) 1184, 2730, 2855
BsmI GAATGC 3 cut(s) 196, 346, 2064
BsnI GGCC 8 cut(s) 60, 330, 500, 881, 1092, 2001, 2292, 2588
BsoBI CYCGRG 1 cut(s) 2882
Bsp1286I GDGCHC 6 cut(s) 108, 775, 1626, 1954, 2467, 2831
Bsp68I TCGCGA 1 cut(s) 1155
BspACI CCGC 8 cut(s) 51, 61, 63, 305, 756, 1333, 1823, 2854
BspANI GGCC 8 cut(s) 60, 330, 500, 881, 1092, 2001, 2292, 2588
BspCNI CTCAG 6 cut(s) 487, 838, 1186, 1236, 1497, 2128
BspFNI CGCG 6 cut(s) 53, 63, 307, 1155, 2856, 2923
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 7 cut(s) 233, 259, 1172, 1896, 2589, 2746, 2778
BspMAI CTGCAG 1 cut(s) 2697
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 5 cut(s) 215, 2012, 2126, 2697, 2845
BspQI GCTCTTC 1 cut(s) 828
BspT107I GGYRCC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 1806
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 2 cut(s) 796, 1665
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 6 cut(s) 61, 188, 1515, 1781, 1897, 2883
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 1515, 1781
Bst2UI CCWGG 4 cut(s) 225, 352, 1899, 2126
Bst6I CTCTTC 2 cut(s) 828, 1569
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 2 cut(s) 620, 643
BstBAI YACGTR 2 cut(s) 75, 2654
BstC8I GCNNGC 7 cut(s) 247, 288, 302, 1827, 2064, 2142, 2821
BstDEI CTNAG 8 cut(s) 495, 846, 1194, 1223, 1408, 1505, 2136, 2241
BstDSI CCRYGG 1 cut(s) 61
BstFNI CGCG 6 cut(s) 53, 63, 307, 1155, 2856, 2923
BstHHI GCGC 2 cut(s) 1809, 1962
BstMAI GTCTC 2 cut(s) 1540, 1832
BstMWI GCNNNNNNNGC 8 cut(s) 620, 643, 827, 1142, 1199, 1655, 1742, 2624
BstNI CCWGG 4 cut(s) 225, 352, 1899, 2126
BstNSI RCATGY 4 cut(s) 290, 1829, 2219, 2618
BstSCI CCNGG 6 cut(s) 187, 223, 350, 661, 1897, 2124
BstSFI CTRYAG 2 cut(s) 2693, 2781
BstSLI GKGCMC 4 cut(s) 1626, 1954, 2467, 2831
BstUI CGCG 6 cut(s) 53, 63, 307, 1155, 2856, 2923
BstV1I GCAGC 5 cut(s) 70, 1120, 1123, 1186, 1241
BstX2I RGATCY 2 cut(s) 2689, 2859
BstXI CCANNNNNNTGG 1 cut(s) 877
BstYI RGATCY 2 cut(s) 2689, 2859
Bsu36I CCTNAGG 1 cut(s) 495
BsuRI GGCC 8 cut(s) 60, 330, 500, 881, 1092, 2001, 2292, 2588
BtgI CCRYGG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 5 cut(s) 235, 685, 1293, 1443, 1658
BtuMI TCGCGA 1 cut(s) 1155
BveI ACCTGC 1 cut(s) 7
Cac8I GCNNGC 7 cut(s) 247, 288, 302, 1827, 2064, 2142, 2821
CciI TCATGA 1 cut(s) 504
CfoI GCGC 2 cut(s) 1809, 1962
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 6 cut(s) 31, 1171, 1894, 2000, 2587, 2744
Cfr42I CCGCGG 1 cut(s) 64
CseI GACGC 2 cut(s) 1742, 1849
Csp6I GTAC 7 cut(s) 46, 258, 402, 716, 1187, 1405, 2901
CspAI ACCGGT 1 cut(s) 260
CviQI GTAC 7 cut(s) 46, 258, 402, 716, 1187, 1405, 2901
DdeI CTNAG 8 cut(s) 495, 846, 1194, 1223, 1408, 1505, 2136, 2241
DraI TTTAAA 3 cut(s) 762, 1975, 2298
DraIII CACNNNGTG 1 cut(s) 2435
EaeI YGGCCR 1 cut(s) 879
Eam1104I CTCTTC 2 cut(s) 828, 1569
EarI CTCTTC 2 cut(s) 828, 1569
Eco130I CCWWGG 2 cut(s) 1515, 1781
Eco147I AGGCCT 2 cut(s) 330, 500
Eco47I GGWCC 4 cut(s) 31, 1171, 1894, 2744
Eco57I CTGAAG 5 cut(s) 876, 950, 1304, 1682, 2172
Eco72I CACGTG 1 cut(s) 75
Eco81I CCTNAGG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 2882
EcoO109I RGGNCCY 1 cut(s) 1894
EcoRI GAATTC 2 cut(s) 1462, 2006
EcoRII CCWGG 4 cut(s) 223, 350, 1897, 2124
EcoT14I CCWWGG 2 cut(s) 1515, 1781
EcoT22I ATGCAT 2 cut(s) 288, 2100
ErhI CCWWGG 2 cut(s) 1515, 1781
Esp3I CGTCTC 1 cut(s) 1832
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 3 cut(s) 1184, 2730, 2855
FauNDI CATATG 1 cut(s) 1738
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 2784
Fnu4HI GCNGC 8 cut(s) 51, 61, 84, 305, 1134, 1137, 1200, 1230
Fsp4HI GCNGC 8 cut(s) 51, 61, 84, 305, 1134, 1137, 1200, 1230
FspBI CTAG 7 cut(s) 38, 114, 546, 624, 1593, 1782, 2727
GlaI GCGC 2 cut(s) 1808, 1961
GluI GCNGC 8 cut(s) 51, 61, 84, 305, 1134, 1137, 1200, 1230
GsuI CTGGAG 6 cut(s) 246, 756, 813, 1682, 1920, 2108
HaeIII GGCC 8 cut(s) 60, 330, 500, 881, 1092, 2001, 2292, 2588
HapII CCGG 7 cut(s) 87, 133, 188, 261, 641, 663, 1796
HgaI GACGC 2 cut(s) 1742, 1849
HhaI GCGC 2 cut(s) 1809, 1962
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 2 cut(s) 1807, 1960
HinP1I GCGC 2 cut(s) 1807, 1960
HpaII CCGG 7 cut(s) 87, 133, 188, 261, 641, 663, 1796
HphI GGTGA 2 cut(s) 677, 1868
Hpy166II GTNNAC 9 cut(s) 31, 1392, 1624, 1820, 1876, 1906, 1952, 2612, 2785
Hpy8I GTNNAC 9 cut(s) 31, 1392, 1624, 1820, 1876, 1906, 1952, 2612, 2785
HpyCH4IV ACGT 4 cut(s) 74, 96, 1388, 2653
HpyF10VI GCNNNNNNNGC 8 cut(s) 620, 643, 827, 1142, 1199, 1655, 1742, 2624
HpyF3I CTNAG 8 cut(s) 495, 846, 1194, 1223, 1408, 1505, 2136, 2241
HpySE526I ACGT 4 cut(s) 74, 96, 1388, 2653
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 2 cut(s) 1807, 1960
KpnI GGTACC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 973
KspI CCGCGG 1 cut(s) 64
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 9 cut(s) 193, 231, 297, 634, 655, 737, 1717, 2782, 2796
Lsp1109I GCAGC 5 cut(s) 70, 1120, 1123, 1186, 1241
LweI GCATC 9 cut(s) 601, 646, 855, 1012, 1241, 1300, 1794, 2413, 2800
MaeI CTAG 7 cut(s) 38, 114, 546, 624, 1593, 1782, 2727
MaeII ACGT 4 cut(s) 74, 96, 1388, 2653
MaeIII GTNAC 4 cut(s) 565, 626, 1508, 2647
MflI RGATCY 2 cut(s) 2689, 2859
MhlI GDGCHC 6 cut(s) 108, 775, 1626, 1954, 2467, 2831
MlyI GAGTC 5 cut(s) 75, 573, 1505, 1760, 2953
MmeI TCCRAC 5 cut(s) 104, 130, 588, 1413, 2784
Mph1103I ATGCAT 2 cut(s) 288, 2100
MroXI GAANNNNTTC 1 cut(s) 393
MslI CAYNNNNRTG 2 cut(s) 77, 875
MspA1I CMGCKG 2 cut(s) 63, 1031
MspI CCGG 7 cut(s) 87, 133, 188, 261, 641, 663, 1796
MspR9I CCNGG 6 cut(s) 189, 225, 352, 663, 1899, 2126
Mva1269I GAATGC 3 cut(s) 196, 346, 2064
MvaI CCWGG 4 cut(s) 225, 352, 1899, 2126
MvnI CGCG 6 cut(s) 53, 63, 307, 1155, 2856, 2923
MwoI GCNNNNNNNGC 8 cut(s) 620, 643, 827, 1142, 1199, 1655, 1742, 2624
NciI CCSGG 2 cut(s) 189, 663
NdeI CATATG 1 cut(s) 1738
NlaIV GGNNCC 7 cut(s) 233, 259, 1172, 1896, 2589, 2746, 2778
NmeAIII GCCGAG 1 cut(s) 1118
NmuCI GTSAC 1 cut(s) 1508
NruI TCGCGA 1 cut(s) 1155
NsiI ATGCAT 2 cut(s) 288, 2100
NspI RCATGY 4 cut(s) 290, 1829, 2219, 2618
PaeI GCATGC 2 cut(s) 290, 1829
PagI TCATGA 1 cut(s) 504
PceI AGGCCT 2 cut(s) 330, 500
PciSI GCTCTTC 1 cut(s) 828
PctI GAATGC 3 cut(s) 196, 346, 2064
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 8 cut(s) 5, 155, 202, 532, 744, 986, 2723, 2735
PflMI CCANNNNNTGG 1 cut(s) 2837
PfoI TCCNGGA 1 cut(s) 223
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 8 cut(s) 52, 62, 85, 306, 1135, 1138, 1201, 1231
PleI GAGTC 5 cut(s) 74, 573, 1505, 1759, 2952
PmaCI CACGTG 1 cut(s) 75
PmlI CACGTG 1 cut(s) 75
PpsI GAGTC 5 cut(s) 74, 573, 1505, 1759, 2952
Ppu21I YACGTR 2 cut(s) 75, 2654
PpuMI RGGWCCY 1 cut(s) 1894
Psp5II RGGWCCY 1 cut(s) 1894
Psp6I CCWGG 4 cut(s) 223, 350, 1897, 2124
PspCI CACGTG 1 cut(s) 75
PspGI CCWGG 4 cut(s) 223, 350, 1897, 2124
PspN4I GGNNCC 7 cut(s) 233, 259, 1172, 1896, 2589, 2746, 2778
PspPI GGNCC 6 cut(s) 31, 1171, 1894, 2000, 2587, 2744
PspPPI RGGWCCY 1 cut(s) 1894
PstI CTGCAG 1 cut(s) 2697
PsuI RGATCY 2 cut(s) 2689, 2859
PvuII CAGCTG 1 cut(s) 1031
RruI TCGCGA 1 cut(s) 1155
RsaI GTAC 7 cut(s) 47, 259, 403, 717, 1188, 1406, 2902
RsaNI GTAC 7 cut(s) 46, 258, 402, 716, 1187, 1405, 2901
RseI CAYNNNNRTG 2 cut(s) 77, 875
SacII CCGCGG 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 828
SatI GCNGC 8 cut(s) 51, 61, 84, 305, 1134, 1137, 1200, 1230
Sau96I GGNCC 6 cut(s) 31, 1171, 1894, 2000, 2587, 2744
ScaI AGTACT 1 cut(s) 2902
SchI GAGTC 5 cut(s) 75, 573, 1505, 1760, 2953
ScrFI CCNGG 6 cut(s) 189, 225, 352, 663, 1899, 2126
SduI GDGCHC 6 cut(s) 108, 775, 1626, 1954, 2467, 2831
SfaNI GCATC 9 cut(s) 601, 646, 855, 1012, 1241, 1300, 1794, 2413, 2800
SfcI CTRYAG 2 cut(s) 2693, 2781
Sfr303I CCGCGG 1 cut(s) 64
SgrBI CCGCGG 1 cut(s) 64
SinI GGWCC 4 cut(s) 31, 1171, 1894, 2744
SmiI ATTTAAAT 1 cut(s) 1975
SmiMI CAYNNNNRTG 2 cut(s) 77, 875
SmlI CTYRAG 2 cut(s) 2182, 2670
SmoI CTYRAG 2 cut(s) 2182, 2670
SpeI ACTAGT 1 cut(s) 113
SphI GCATGC 2 cut(s) 290, 1829
SseBI AGGCCT 2 cut(s) 330, 500
SsiI CCGC 8 cut(s) 51, 61, 63, 305, 756, 1333, 1823, 2854
SspI AATATT 2 cut(s) 2529, 2894
SspMI CTAG 7 cut(s) 38, 114, 546, 624, 1593, 1782, 2727
StuI AGGCCT 2 cut(s) 330, 500
StyD4I CCNGG 6 cut(s) 187, 223, 350, 661, 1897, 2124
StyI CCWWGG 2 cut(s) 1515, 1781
SwaI ATTTAAAT 1 cut(s) 1975
TaiI ACGT 4 cut(s) 77, 99, 1391, 2656
TaqI TCGA 7 cut(s) 162, 200, 380, 1269, 1341, 1567, 1814
TatI WGTACW 4 cut(s) 715, 1186, 1404, 2900
TauI GCSGC 3 cut(s) 53, 63, 307
TfiI GAWTC 8 cut(s) 5, 155, 202, 532, 744, 986, 2723, 2735
TscAI CASTG 5 cut(s) 242, 685, 1300, 1450, 1665
TseFI GTSAC 1 cut(s) 1508
TseI GCWGC 5 cut(s) 83, 1133, 1136, 1199, 1229
Tsp45I GTSAC 1 cut(s) 1508
TspGWI ACGGA 3 cut(s) 426, 555, 2272
TspRI CASTG 5 cut(s) 242, 685, 1300, 1450, 1665
Van91I CCANNNNNTGG 1 cut(s) 2837
VneI GTGCAC 2 cut(s) 1622, 1950
VpaK11BI GGWCC 4 cut(s) 31, 1171, 1894, 2744
XapI RAATTY 6 cut(s) 763, 802, 890, 1462, 1976, 2006
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 4 cut(s) 290, 1829, 2219, 2618
XcmI CCANNNNNNNNNTGG 1 cut(s) 1522
XmaJI CCTAGG 1 cut(s) 1781
XmiI GTMKAC 1 cut(s) 2784
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 7 cut(s) 38, 114, 546, 624, 1593, 1782, 2727
ZraI GACGTC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 2902
Zsp2I ATGCAT 2 cut(s) 288, 2100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.