MD17G1084700.v1.1

vacuolar protein sorting-associated protein 18 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016149
Physical Location & Seq
Forward (+)
6967200 .. 6982033
14834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1084700.v1.1.491

Sequence Viewer

Length: 2958 bp
ATGGATTCAGGGAGGCAGGTGTTCACGGTGGATCTTCTGGAACGATATGCGGCCAAGGGGCGCGGAAATATCACCTGTATGGCTGCCGGAAACGACGTCATTTTGCTCGGAACCAGTAAAGGTTGGATCATTCGACACGATTTCGGCGTCGGCGATTCGTTTGATATTGATCTCTCGGTGGGTCGTCCTGGGGAGCAATCCATCCATAGAGTTTTTGTTGATCCTGGAGGGAGCCACTGCATTGCCACCATTGTTGGGAGTGGGGGTGCTGACACGTTCTATACTCATGCAAAATGGACCAAGCCCCGTCTTTTAACCAAGCTAAAGGGTCTTGTTATGAATGCCGTTGCCTGGAACAGACAACAGATTACAGAAGCTTCAACAAAGGAAGTCATTCTCGGTACAGACAACGGCCAACTTTATGAAATGGCAGTTGACGAGAAGGATAAGAAGGAGAAATACGTAAAGTTTTTGTTCGAATTATTAGAACTTCCTGAGGCTTTCATGGGTTTGCAGATGGAAACGGCAACCATTCTCAGTGGGACTAGATACTATGTAATGGCTGTAACTCCTACAAGACTCTATTCTTTTACTGGTATTGGATCACTGGAAACTGTTTTTGCTAGTTACTCGGAACAAACAGTGCATTTCATGGAACTTCCTGGTGAAATTCCTAACAGTGAACTGCATTTCTATATCAAGCAAAGAAGAGCAATACATTTTGCATGGCTTTCTGGTGCTGGTATATATCATGGTGGTTTAAATTTTGGAGCACAACATAGTTCTCCTGATGGAGATGAAAATTTTGTGGAGAACAAGGCTCTTTTGAACTATTCAACTTTGAGTGGGGGTTCTGAAGCGGTGAAACCCAGTTCTATGGCAGTTTCAGAATTTCATTTCCTGCTTCTTATCGGGAATAGGGTTAAGGTTGTAAATAGAATAAGTGAGCAAACTATTGAGGAGCTTCAGTTTGAGCAAACACCCGAAGCAGTTTCAAGGGGTGTCACTGGATTGTGTAGTGATGCCACTGCTGGGTTGTTCTATGCATATGATCAAAACTCTGTATTTCAGGTGTCTGTTAATGATGAAGGCCGAGACATGTGGAAAGTTTACCTGGACATGAAGGAGTATGCTGCTGCTTTGGCAAATTGCCGCGACCCACTTCAGAGGGACCAAGTATATTTGGCTGAAGCTGCATTTTCCGCCAAGGATTATCTTAGAGCAGCATCAGTCTATGCAAAAATTAATTACATATTGTCATTCGAGGAGATCACTTTGAAGTTTATCACTGTAAATGAACAGGATGCTTTAAGAACCTTTCTATTGTGGAAACTTGATAGCCTTGCGGTGGATGACAAATGCCAAGTAACAATGATTTCGACATGGGCAACTGAACTGTACTTGGATAAGATAAATCGTCTACTATTGGAAGATGATACTGCAGTGGATAATCGTAATTCTGAGTATCACTTGATCATTAAGGAGTTTCGTGCATTTCTTAGTGACTCCAAGGATGTTTTGGATGAGGCAACTACCATGAGACTTTTAGAAAGTTATGGCCGAGTCGAAGAATTGGTTTTTTTTGCTAGTCTGAAGGAACAGTATGAAATTGTAGTCCACCATTATATTCAGCAAGGAGAGGCAAAGAAAGCATTGGAGGTGCTCCAAAAACCTACTGTTCCTATAGATCTTCAGTACAAGTTTGCACCAGACCTAATCATGCTTGATGCATATGAAGCTGTGGAGTCATGGATGACTACAAACAACCTCAATCCAAGGAAACTCATTCCTGCAATGATGCGTTATTCAAGTGAACCACATGCAAGAAATGAAACACACGAAGTCATCAAATATTTGGAATACTGTGTTCATCGTTTGCATAATGAGGATCCTGGTGTTCACAACTTGCTATTATCACTATATGCCAAACAGGAAGACGACGGTGCACTTTTGCGTTTCCTTCAATTCAAGTTCGGAAAAGGACGGGAAATTGGCCCAGAGTTCTTCTATGATCCCAAGTATGCTTTACGCCTTTGCCTCAAGGAAAAGCGAATGCGTGCCTGTGTTCATATATATAGTATGATGTCCATGCATGAAGAAGCAGTTGCTCTTGCCCTGCAGGTTGATCCTGAGCTTGCTATGGCTGAGGCTGACAAGGTTGAAGATGATGAGGACCTGAGAAAGAAGCTTTGGCTCATGGTTGCGAAGCATGTTATAGAACAGGAAAGGGGAGCTAAAAGGGAGAACATTCGGAAGGCAATAGCATTTCTTAAGGAAACTGATGGCCTTTTAAAGATAGAGGATATATTACCGTTCTTCCCGGACTTTGCCCTCATTGACGACTTCAAGGAGGCAATTTGCTCGTCATTAGAGGATTACAACAATCAAATTGAAGAACTGAAGCAAGAGATGAATGATGCAACACATGGTGCCGATAACATTAGAAATGACATTAGTGCACTTGCTCAAAGATATGCTGTGATTGATCGTGATGAAGAATGCGGGGTTTGTCGGCGTAAGATTTTAACTGTGGGTAGGGAGTATCAGTTGGCTAGAGGCTATGCATCCGTAGGCCAAATGGCTCCATTCTATGTGTTTCCATGCGGACATGCCTTCCATGCCGAATGCCTGATTGCCCACGTCACACGTAGTACAAATGAAGCTCAAGCAGAGTATATATTGGATCTGCAGAAGCAGCTTACTCTACTTGACGGGGAAGCTAGGAAGGATTCAAATGGTAGCCTTACTGAGGAAACCATAACAAGCATGGCCCCCGTAGATAAGCTGCGGTCGCAGTTGGATGATGCAGTGGCCAGCGAATGCCCATTTTGTGGGGACTTGATGATCCGTGAGATCTCTCTACCTTTCATCCTGCCTGTGGAGCAACAGCAGAATACTTCATGGGAGATAAAGTCGCATAATCTTGGACACCAGAGGAGCCTGGGTTTATCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006904 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007275 GO:0007610 GO:0007632 GO:0007634 GO:0008104 GO:0008150 GO:0008333 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009653 GO:0009705 GO:0009790 GO:0009987 GO:0010015 GO:0010256 GO:0010876 GO:0012505 GO:0015711 GO:0015718 GO:0015721 GO:0015849 GO:0015850 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0021700 GO:0022406 GO:0022622 GO:0030154 GO:0030674 GO:0030897 GO:0030903 GO:0031090 GO:0031338 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032879 GO:0032940 GO:0032991 GO:0033036 GO:0033043 GO:0033059 GO:0033263 GO:0035542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043473 GO:0043476 GO:0043482 GO:0043485 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045176 GO:0046903 GO:0046907 GO:0046942 GO:0048066 GO:0048069 GO:0048278 GO:0048364 GO:0048468 GO:0048513 GO:0048568 GO:0048731 GO:0048757 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0050931 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0051716 GO:0060034 GO:0060035 GO:0060036 GO:0060090 GO:0060627 GO:0065007 GO:0071702 GO:0071840 GO:0080171 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099402 GO:0140029 GO:0140056 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin Pep3_Vps18

Protein Analysis

986

Amino Acids

111.58

Weight (kDa)

5.15

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pep3_Vps18 PF05131 15 - 360 2.5e-83 Pep3/Vps18/deep orange beta-propeller domain
Clathrin PF00637 605 - 743 4.6e-08 Region in Clathrin and VPS
VPS18_RING_C PF26148 863 - 950 9.2e-07 VPS18 RING C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12470
fragaria_vesca FvH4_6g44560 FvH4_6g44560
malus_domestica MD14G1065300.v1.1 MD17G1084700.v1.1
prunus_persica Prupe.3G231000_v2.0.a1
pyrus_communis pycom17g08190
rosa_chinensis RchiOBHm_Chr2g0161771
rosa_laevigata RLG00000021298
rosa_multiflora Rmu_sc0003662.1_g000015
rosa_roxburghii Rroxscaffold_2G00088620
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2BG571500 Rh2DG580400
rosa_wichuraiana Rw2G046160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 7
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 2 cut(s) 7, 2110
AccB1I GGYRCC 1 cut(s) 2429
AccB7I CCANNNNNTGG 1 cut(s) 2831
AccI GTMKAC 1 cut(s) 1420
AccII CGCG 2 cut(s) 63, 1155
AciI CCGC 9 cut(s) 50, 63, 860, 1153, 1203, 1346, 2502, 2604, 2788
AcoI YGGCCR 4 cut(s) 51, 412, 1558, 2811
AcsI RAATTY 4 cut(s) 669, 763, 802, 890
AcuI CTGAAG 7 cut(s) 876, 950, 1148, 1209, 1613, 1676, 2420
AcyI GRCGYC 2 cut(s) 96, 147
AdeI CACNNNGTG 1 cut(s) 2429
AfaI GTAC 4 cut(s) 403, 1400, 1697, 2653
AfiI CCNNNNNNNGG 7 cut(s) 255, 351, 1032, 1348, 2749, 2831, 2878
AflII CTTAAG 1 cut(s) 2270
AflIII ACRYGT 3 cut(s) 273, 1098, 2645
AjiI CACGTC 1 cut(s) 2641
AjnI CCWGG 7 cut(s) 187, 223, 350, 661, 1113, 1891, 2940
AjuI GAANNNNNNNTTGG 2 cut(s) 2173, 2205
Alw21I GWGCWC 4 cut(s) 775, 1665, 1948, 2461
Alw26I GTCTC 2 cut(s) 1089, 1534
Alw44I GTGCAC 2 cut(s) 1944, 2457
AoxI GGCC 9 cut(s) 51, 412, 1090, 1558, 1993, 2284, 2572, 2769, 2811
ApaLI GTGCAC 2 cut(s) 1944, 2457
ApeKI GCWGC 7 cut(s) 83, 1133, 1136, 1193, 1223, 2695, 2785
ApoI RAATTY 4 cut(s) 669, 763, 802, 890
AseI ATTAAT 1 cut(s) 1245
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 5 cut(s) 297, 1171, 1994, 2173, 2770
AsuC2I CCSGG 1 cut(s) 2321
AsuHPI GGTGA 3 cut(s) 64, 677, 874
AsuII TTCGAA 1 cut(s) 477
AvaII GGWCC 3 cut(s) 297, 1171, 2173
AxyI CCTNAGG 1 cut(s) 495
BaeGI GKGCMC 2 cut(s) 1948, 2461
BaeI ACNNNNGTAYC 2 cut(s) 1428, 1461
BalI TGGCCA 1 cut(s) 2813
BamHI GGATCC 1 cut(s) 1888
BanI GGYRCC 1 cut(s) 2429
BarI GAAGNNNNNNTAC 2 cut(s) 443, 475
BbsI GAAGAC 1 cut(s) 1941
Bbv12I GWGCWC 4 cut(s) 775, 1665, 1948, 2461
BbvCI CCTCAGC 1 cut(s) 2145
BbvI GCAGC 7 cut(s) 70, 1120, 1123, 1180, 1235, 2707, 2772
BccI CCATC 4 cut(s) 209, 511, 785, 2276
BceAI ACGGC 3 cut(s) 329, 427, 540
BcgI CGANNNNNNTGC 2 cut(s) 2343, 2377
BciT130I CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1893, 2942
BclI TGATCA 2 cut(s) 1051, 1473
BcnI CCSGG 1 cut(s) 2321
BcoDI GTCTC 2 cut(s) 1089, 1534
BfaI CTAG 5 cut(s) 546, 624, 1587, 2553, 2721
BfmI CTRYAG 4 cut(s) 1440, 1683, 2117, 2687
BfrI CTTAAG 1 cut(s) 2270
BfuAI ACCTGC 2 cut(s) 7, 2110
BglII AGATCT 2 cut(s) 1687, 2853
BisI GCNGC 9 cut(s) 51, 84, 1134, 1137, 1153, 1194, 1224, 2696, 2786
BlsI GCNGC 9 cut(s) 52, 85, 1135, 1138, 1154, 1195, 1225, 2697, 2787
Bme1390I CCNGG 8 cut(s) 189, 225, 352, 663, 1115, 1893, 2321, 2942
Bme18I GGWCC 3 cut(s) 297, 1171, 2173
BmgBI CACGTC 1 cut(s) 2641
BmgT120I GGNCC 5 cut(s) 297, 1171, 1994, 2173, 2770
BmiI GGNNCC 8 cut(s) 112, 233, 1172, 1890, 2431, 2583, 2772, 2939
BmrFI CCNGG 8 cut(s) 189, 225, 352, 663, 1115, 1893, 2321, 2942
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 8 cut(s) 1012, 1235, 1294, 1717, 1788, 2407, 2573, 2794
BmuI ACTGGG 1 cut(s) 864
BpiI GAAGAC 1 cut(s) 1941
BpmI CTGGAG 1 cut(s) 246
Bpu10I CCTNAGC 2 cut(s) 2130, 2145
Bpu14I TTCGAA 1 cut(s) 477
BpuEI CTTGAG 2 cut(s) 2024, 2649
BpuMI CCSGG 1 cut(s) 2321
BsaAI YACGTR 2 cut(s) 463, 2648
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 2 cut(s) 96, 147
BsaJI CCNNGG 6 cut(s) 54, 188, 1206, 1509, 1775, 2941
BsaXI ACNNNNNCTCC 9 cut(s) 250, 280, 953, 983, 2531, 2561, 2897, 2927, 2929
Bsc4I CCNNNNNNNGG 7 cut(s) 255, 351, 1032, 1348, 2749, 2831, 2878
Bse1I ACTGG 5 cut(s) 114, 598, 612, 870, 1012
Bse21I CCTNAGG 1 cut(s) 495
Bse3DI GCAATG 2 cut(s) 240, 1800
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1893, 2942
BseDI CCNNGG 6 cut(s) 54, 188, 1206, 1509, 1775, 2941
BseGI GGATG 9 cut(s) 201, 1309, 1357, 1519, 1528, 1758, 2564, 2806, 2868
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 7 cut(s) 255, 351, 1032, 1348, 2749, 2831, 2878
BseMI GCAATG 2 cut(s) 240, 1800
BseMII CTCAG 7 cut(s) 486, 550, 1452, 2121, 2136, 2168, 2739
BseNI ACTGG 5 cut(s) 114, 598, 612, 870, 1012
BseRI GAGGAG 3 cut(s) 974, 1280, 2950
BseSI GKGCMC 2 cut(s) 1948, 2461
BseXI GCAGC 7 cut(s) 70, 1120, 1123, 1180, 1235, 2707, 2772
BseYI CCCAGC 1 cut(s) 1031
Bsh1236I CGCG 2 cut(s) 63, 1155
Bsh1285I CGRYCG 1 cut(s) 2792
BshFI GGCC 9 cut(s) 53, 414, 1092, 1560, 1995, 2286, 2574, 2771, 2813
BshNI GGYRCC 1 cut(s) 2429
BsiEI CGRYCG 1 cut(s) 2792
BsiHKAI GWGCWC 4 cut(s) 775, 1665, 1948, 2461
BsiSI CCGG 2 cut(s) 87, 2321
BslFI GGGAC 3 cut(s) 556, 1184, 2849
BslI CCNNNNNNNGG 7 cut(s) 255, 351, 1032, 1348, 2749, 2831, 2878
BsmAI GTCTC 2 cut(s) 1089, 1534
BsmFI GGGAC 3 cut(s) 556, 1184, 2849
BsmI GAATGC 5 cut(s) 346, 2058, 2504, 2630, 2825
BsnI GGCC 9 cut(s) 53, 414, 1092, 1560, 1995, 2286, 2574, 2771, 2813
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 4 cut(s) 775, 1665, 1948, 2461
BspACI CCGC 9 cut(s) 50, 63, 860, 1153, 1203, 1346, 2502, 2604, 2788
BspANI GGCC 9 cut(s) 53, 414, 1092, 1560, 1995, 2286, 2574, 2771, 2813
BspCNI CTCAG 7 cut(s) 487, 549, 1453, 2122, 2137, 2169, 2740
BspFNI CGCG 2 cut(s) 63, 1155
BspLI GGNNCC 8 cut(s) 112, 233, 1172, 1890, 2431, 2583, 2772, 2939
BspMAI CTGCAG 3 cut(s) 1444, 2121, 2691
BspMI ACCTGC 2 cut(s) 7, 2110
BspQI GCTCTTC 1 cut(s) 703
BspT104I TTCGAA 1 cut(s) 477
BspT107I GGYRCC 1 cut(s) 2429
BspTI CTTAAG 1 cut(s) 2270
BsrDI GCAATG 2 cut(s) 240, 1800
BsrI ACTGG 5 cut(s) 114, 598, 612, 870, 1012
BssECI CCNNGG 6 cut(s) 54, 188, 1206, 1509, 1775, 2941
BssNI GRCGYC 2 cut(s) 96, 147
BssT1I CCWWGG 4 cut(s) 54, 1206, 1509, 1775
Bst2UI CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1893, 2942
Bst6I CTCTTC 1 cut(s) 703
BstACI GRCGYC 2 cut(s) 96, 147
BstAFI CTTAAG 1 cut(s) 2270
BstBAI YACGTR 2 cut(s) 463, 2648
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 3 cut(s) 2058, 2136, 2815
BstDEI CTNAG 9 cut(s) 495, 536, 1217, 1461, 1499, 2130, 2145, 2177, 2748
BstENI CCTNNNNNAGG 1 cut(s) 2747
BstF5I GGATG 9 cut(s) 201, 1309, 1357, 1519, 1528, 1758, 2564, 2806, 2868
BstFNI CGCG 2 cut(s) 63, 1155
BstHHI GCGC 1 cut(s) 63
BstMAI GTCTC 2 cut(s) 1089, 1534
BstMCI CGRYCG 1 cut(s) 2792
BstMWI GCNNNNNNNGC 9 cut(s) 1142, 1193, 1202, 1649, 1736, 2618, 2695, 2791, 2881
BstNI CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1893, 2942
BstNSI RCATGY 4 cut(s) 1102, 1823, 2213, 2612
BstSCI CCNGG 8 cut(s) 187, 223, 350, 661, 1113, 1891, 2319, 2940
BstSFI CTRYAG 4 cut(s) 1440, 1683, 2117, 2687
BstSLI GKGCMC 2 cut(s) 1948, 2461
BstSNI TACGTA 1 cut(s) 463
BstUI CGCG 2 cut(s) 63, 1155
BstV1I GCAGC 7 cut(s) 70, 1120, 1123, 1180, 1235, 2707, 2772
BstV2I GAAGAC 1 cut(s) 1941
BstX2I RGATCY 5 cut(s) 31, 1687, 1888, 2683, 2853
BstXI CCANNNNNNTGG 1 cut(s) 877
BstYI RGATCY 5 cut(s) 31, 1687, 1888, 2683, 2853
Bsu36I CCTNAGG 1 cut(s) 495
BsuRI GGCC 9 cut(s) 53, 414, 1092, 1560, 1995, 2286, 2574, 2771, 2813
BtrI CACGTC 1 cut(s) 2641
BtsCI GGATG 9 cut(s) 201, 1309, 1357, 1519, 1528, 1758, 2564, 2806, 2868
BtsI GCAGTG 4 cut(s) 235, 1026, 1449, 2814
BveI ACCTGC 2 cut(s) 7, 2110
Cac8I GCNNGC 3 cut(s) 2058, 2136, 2815
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 5 cut(s) 297, 1171, 1994, 2173, 2770
CseI GACGC 1 cut(s) 136
Csp6I GTAC 4 cut(s) 402, 1399, 1696, 2652
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviQI GTAC 4 cut(s) 402, 1399, 1696, 2652
DdeI CTNAG 9 cut(s) 495, 536, 1217, 1461, 1499, 2130, 2145, 2177, 2748
DraI TTTAAA 2 cut(s) 762, 2292
DraIII CACNNNGTG 1 cut(s) 2429
EaeI YGGCCR 4 cut(s) 51, 412, 1558, 2811
Eam1104I CTCTTC 1 cut(s) 703
EarI CTCTTC 1 cut(s) 703
EciI GGCGGA 1 cut(s) 1192
Eco105I TACGTA 1 cut(s) 463
Eco130I CCWWGG 4 cut(s) 54, 1206, 1509, 1775
Eco47I GGWCC 3 cut(s) 297, 1171, 2173
Eco57I CTGAAG 7 cut(s) 876, 950, 1148, 1209, 1613, 1676, 2420
Eco81I CCTNAGG 1 cut(s) 495
EcoNI CCTNNNNNAGG 1 cut(s) 2747
EcoO109I RGGNCCY 1 cut(s) 2173
EcoRII CCWGG 7 cut(s) 187, 223, 350, 661, 1113, 1891, 2940
EcoT14I CCWWGG 4 cut(s) 54, 1206, 1509, 1775
EcoT22I ATGCAT 4 cut(s) 1048, 1732, 2094, 2566
ErhI CCWWGG 4 cut(s) 54, 1206, 1509, 1775
FaqI GGGAC 3 cut(s) 556, 1184, 2849
FauI CCCGC 1 cut(s) 2495
FauNDI CATATG 2 cut(s) 1048, 1732
FbaI TGATCA 2 cut(s) 1051, 1473
FblI GTMKAC 1 cut(s) 1420
Fnu4HI GCNGC 9 cut(s) 51, 84, 1134, 1137, 1153, 1194, 1224, 2696, 2786
FokI GGATG 9 cut(s) 188, 1316, 1364, 1526, 1535, 1765, 2551, 2813, 2855
Fsp4HI GCNGC 9 cut(s) 51, 84, 1134, 1137, 1153, 1194, 1224, 2696, 2786
FspBI CTAG 5 cut(s) 546, 624, 1587, 2553, 2721
GlaI GCGC 1 cut(s) 62
GluI GCNGC 9 cut(s) 51, 84, 1134, 1137, 1153, 1194, 1224, 2696, 2786
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 9 cut(s) 53, 414, 1092, 1560, 1995, 2286, 2574, 2771, 2813
HapII CCGG 2 cut(s) 87, 2321
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 2 cut(s) 96, 147
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HindIII AAGCTT 2 cut(s) 375, 2186
HinfI GANTC 7 cut(s) 5, 155, 579, 1505, 1563, 1745, 2729
HpaII CCGG 2 cut(s) 87, 2321
HphI GGTGA 3 cut(s) 64, 677, 874
Hpy188I TCNGA 9 cut(s) 110, 634, 856, 889, 1167, 1462, 1593, 1976, 2253
Hpy188III TCNNGA 6 cut(s) 38, 494, 788, 913, 2129, 2489
Hpy99I CGWCG 3 cut(s) 98, 152, 1943
HpyAV CCTTC 9 cut(s) 436, 445, 1082, 1117, 1588, 1970, 2248, 2623, 2719
HpyCH4IV ACGT 5 cut(s) 96, 275, 462, 2640, 2647
HpyF10VI GCNNNNNNNGC 9 cut(s) 1142, 1193, 1202, 1649, 1736, 2618, 2695, 2791, 2881
HpyF3I CTNAG 9 cut(s) 495, 536, 1217, 1461, 1499, 2130, 2145, 2177, 2748
HpySE526I ACGT 5 cut(s) 96, 275, 462, 2640, 2647
Hsp92I GRCGYC 2 cut(s) 96, 147
HspAI GCGC 1 cut(s) 61
Ksp22I TGATCA 2 cut(s) 1051, 1473
LguI GCTCTTC 1 cut(s) 703
LmnI GCTCC 9 cut(s) 193, 231, 770, 961, 1668, 2231, 2587, 2881, 2937
Lsp1109I GCAGC 7 cut(s) 70, 1120, 1123, 1180, 1235, 2707, 2772
LweI GCATC 8 cut(s) 1012, 1235, 1294, 1717, 1788, 2407, 2573, 2794
MaeI CTAG 5 cut(s) 546, 624, 1587, 2553, 2721
MaeII ACGT 5 cut(s) 96, 275, 462, 2640, 2647
MaeIII GTNAC 6 cut(s) 565, 626, 1003, 1366, 1502, 2641
MflI RGATCY 5 cut(s) 31, 1687, 1888, 2683, 2853
MhlI GDGCHC 4 cut(s) 775, 1665, 1948, 2461
MlsI TGGCCA 1 cut(s) 2813
MluNI TGGCCA 1 cut(s) 2813
MlyI GAGTC 4 cut(s) 573, 1499, 1572, 1754
MmeI TCCRAC 2 cut(s) 104, 2778
Mox20I TGGCCA 1 cut(s) 2813
Mph1103I ATGCAT 4 cut(s) 1048, 1732, 2094, 2566
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 2813
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 2813
MspCI CTTAAG 1 cut(s) 2270
MspI CCGG 2 cut(s) 87, 2321
MspR9I CCNGG 8 cut(s) 189, 225, 352, 663, 1115, 1893, 2321, 2942
Mva1269I GAATGC 5 cut(s) 346, 2058, 2504, 2630, 2825
MvaI CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1893, 2942
MvnI CGCG 2 cut(s) 63, 1155
MwoI GCNNNNNNNGC 9 cut(s) 1142, 1193, 1202, 1649, 1736, 2618, 2695, 2791, 2881
NciI CCSGG 1 cut(s) 2321
NdeI CATATG 2 cut(s) 1048, 1732
NlaIV GGNNCC 8 cut(s) 112, 233, 1172, 1890, 2431, 2583, 2772, 2939
NmeAIII GCCGAG 2 cut(s) 1118, 1586
NmuCI GTSAC 3 cut(s) 1003, 1502, 2641
NsiI ATGCAT 4 cut(s) 1048, 1732, 2094, 2566
NspI RCATGY 4 cut(s) 1102, 1823, 2213, 2612
NspV TTCGAA 1 cut(s) 477
PaqCI CACCTGC 1 cut(s) 7
PciI ACATGT 1 cut(s) 1098
PciSI GCTCTTC 1 cut(s) 703
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 150
PctI GAATGC 5 cut(s) 346, 2058, 2504, 2630, 2825
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 5, 155, 2729
PflMI CCANNNNNTGG 1 cut(s) 2831
PfoI TCCNGGA 2 cut(s) 223, 2319
PkrI GCNGC 9 cut(s) 52, 85, 1135, 1138, 1154, 1195, 1225, 2697, 2787
PleI GAGTC 4 cut(s) 573, 1499, 1571, 1753
PpsI GAGTC 4 cut(s) 573, 1499, 1571, 1753
Ppu21I YACGTR 2 cut(s) 463, 2648
PpuMI RGGWCCY 1 cut(s) 2173
PscI ACATGT 1 cut(s) 1098
PshBI ATTAAT 1 cut(s) 1245
Psp5II RGGWCCY 1 cut(s) 2173
Psp6I CCWGG 7 cut(s) 187, 223, 350, 661, 1113, 1891, 2940
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 7 cut(s) 187, 223, 350, 661, 1113, 1891, 2940
PspN4I GGNNCC 8 cut(s) 112, 233, 1172, 1890, 2431, 2583, 2772, 2939
PspPI GGNCC 5 cut(s) 297, 1171, 1994, 2173, 2770
PspPPI RGGWCCY 1 cut(s) 2173
PstI CTGCAG 3 cut(s) 1444, 2121, 2691
PsuI RGATCY 5 cut(s) 31, 1687, 1888, 2683, 2853
RsaI GTAC 4 cut(s) 403, 1400, 1697, 2653
RsaNI GTAC 4 cut(s) 402, 1399, 1696, 2652
RseI CAYNNNNRTG 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 703
SatI GCNGC 9 cut(s) 51, 84, 1134, 1137, 1153, 1194, 1224, 2696, 2786
Sau96I GGNCC 5 cut(s) 297, 1171, 1994, 2173, 2770
SbfI CCTGCAGG 1 cut(s) 2121
SchI GAGTC 4 cut(s) 573, 1499, 1572, 1754
ScrFI CCNGG 8 cut(s) 189, 225, 352, 663, 1115, 1893, 2321, 2942
SdaI CCTGCAGG 1 cut(s) 2121
SduI GDGCHC 4 cut(s) 775, 1665, 1948, 2461
SfaNI GCATC 8 cut(s) 1012, 1235, 1294, 1717, 1788, 2407, 2573, 2794
SfcI CTRYAG 4 cut(s) 1440, 1683, 2117, 2687
SfuI TTCGAA 1 cut(s) 477
SinI GGWCC 3 cut(s) 297, 1171, 2173
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 3 cut(s) 2039, 2270, 2664
SmoI CTYRAG 3 cut(s) 2039, 2270, 2664
SnaBI TACGTA 1 cut(s) 463
Sse8387I CCTGCAGG 1 cut(s) 2121
SsiI CCGC 9 cut(s) 50, 63, 860, 1153, 1203, 1346, 2502, 2604, 2788
SspI AATATT 1 cut(s) 1853
SspMI CTAG 5 cut(s) 546, 624, 1587, 2553, 2721
StyD4I CCNGG 8 cut(s) 187, 223, 350, 661, 1113, 1891, 2319, 2940
StyI CCWWGG 4 cut(s) 54, 1206, 1509, 1775
TaiI ACGT 5 cut(s) 99, 278, 465, 2643, 2650
TaqI TCGA 5 cut(s) 133, 477, 1263, 1379, 1566
TatI WGTACW 3 cut(s) 1398, 1695, 2651
TauI GCSGC 2 cut(s) 53, 1155
TfiI GAWTC 3 cut(s) 5, 155, 2729
TseFI GTSAC 3 cut(s) 1003, 1502, 2641
TseI GCWGC 7 cut(s) 83, 1133, 1136, 1193, 1223, 2695, 2785
Tsp45I GTSAC 3 cut(s) 1003, 1502, 2641
TspGWI ACGGA 2 cut(s) 2557, 2837
Van91I CCANNNNNTGG 1 cut(s) 2831
Vha464I CTTAAG 1 cut(s) 2270
VneI GTGCAC 2 cut(s) 1944, 2457
VpaK11BI GGWCC 3 cut(s) 297, 1171, 2173
VspI ATTAAT 1 cut(s) 1245
XagI CCTNNNNNAGG 1 cut(s) 2747
XapI RAATTY 4 cut(s) 669, 763, 802, 890
XceI RCATGY 4 cut(s) 1102, 1823, 2213, 2612
XcmI CCANNNNNNNNNTGG 2 cut(s) 1516, 2764
XmiI GTMKAC 1 cut(s) 1420
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 5 cut(s) 546, 624, 1587, 2553, 2721
ZraI GACGTC 1 cut(s) 97
Zsp2I ATGCAT 4 cut(s) 1048, 1732, 2094, 2566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.