Rw2G046160

vacuolar protein sorting-associated protein 18 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016149
Physical Location & Seq
Reverse (-)
73483691 .. 73493296
9606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G046160.1

Sequence Viewer

Length: 2964 bp
ATGGATTCGGGGCGGCAGGTTTTCACGGTGGACCTTCTAGAAAGGTACGCCGCGAAGGGACGCGGAGTCATCACTTGTATGGCGGCCGGAAACGACGTCATTGTGCTCGGAACTAGTAAAGGATGGATAATCCGGCACGATTTTGGGGTCGGCGATTCGTTCGATATTGATCTCTCTGCGGGTCGTCCCGGGGAGCATTCAATTCATAGAGTTTTTGTTGATCCTGGAGGGAGCCACTGTATCGCCAACATTGCTGGTACCGGGGGTGCCGATACTTTCTATACTCATGCGAAATGGAGCAAGCCGCGTCCTTTGGCCAAGTTGAAAGGTCTTGTGGTGAATGCTGTTGCCTGGAATAGACAGCAGATAACAGAAGTTTCAACAAAGGAAGTTATTCTCGGTACAGACAATGGCCAACTTTATGAGATTGCTGTGGATGAGAAGGATAAGAAGGAGAAGTATGTGAAGTTTCTGTATGAACTAACTGAACTTCCAGAGGCTTTCATGAGTTTGCAGATGGAAACTGCGACAATTCTGAATGGAACTAGATACTATGTGATGGCTGTTACTCCTACACGACTCTATTCCTATACGGGAATTGGATTGTTGGATGCTATTTTTGCTAGTTACTTGGAGCATCCGGTGCGTTTCATGGAACTTCCCGGTGAAATACCTAACAGTGAATTGCATTTCTATATCAAGCAAAGACGAGCAGTACATTTTGCATGGCTTTCTGGAGCTGGAATCTATAATGGCGGTTTAAATTTTGGTTCTCAGCATAGTTCTTCAACTGGAGATGAAAATTTTGTGGAGAACAAAGCTCTTCTGGCATATTCAAAATTGTCTGAGAATACTGAAGTTGCGGTGCCCAGTTCTATGGCCGTTTCGGAATTTCATTTCTTGCTTCTTATCGGGAATAAGGTTAAGGTTGTGAATAGAATAAGTGAGCAAATTATTGAGGAACTTCAGTTTGATCAAACATCTGAATCAGCTTCAAGGGGTATCATTGGATTATGTAGTGATGCCACAGCTGGACTGTTCTATGCTTATGACCAGAACTCTGTATTTCAGGTTTCTGTTAATGATGAAGGCCGAGATATGTGGAAAGTTTATCTGGACATGAAAGAGTATGCTGCTGCTTTGGCAAATTGTCGTGACCCACTTCAGAGGGACCAAGTATATTTAGTACAGGCTGAGGCTGCATTTACCTCAAAAGATTATCTCAGAGCAGCATCTTTCTATGCAAAGATAAACTACATATTATCATTCGAGGAGATCACTCTGAAGTTTATCACTGTCAACGAACAGGACGCTTTGAGAACCTTTTTGTTACGGAAACTTGACTGTCTTGCGAAGGATGACAAGTGCCAAATAACCATGATTTCAACGTGGGCAACTGAATTGTACTTGGATAAGATAAATCGCCTACTATTAGAAGATGACACTGCATTGGAAAATCGGAATTCAGAGTATCAATCAATTATTAAAGAGTTTCGTGCATTTCTGAGTGACTCCAAGGATGTATTGGATGAAGCAACCACGATGAGACTATTAGAAAGTTATGGTCGAGTTGAAGAGTTGGTATTTTTTGCTAGTCTGAAGGAACAATATGAAATTGTAGTGCACCATTATATTCAGCAAGGAGAGGCGAAGAAAGCATTGGAGGTTCTTCAAAAACCTTCTGTTGCTATTGATCTTCAGTATAAGTTTGCTCCTGACCTAATCATGCTTGATGCATACGAAGCTGTGGAGTCATGGATGGCCACAAACAACCTCAACCCTAGGAAACTCATTCCTGCAATGATGCGCTATTCGAGTGAACCACATGCAAAAAATGAAACACATGAAGTCATTAAATACTTGGAGTATTGTGTTCACCGTTTGCACAATGAGGACCCTGGAGTTCACAACTTGCTTTTATCACTATATGCCAAGCAGGAAGATGATAGTGCACTTTTGCGTTTCCTACAATTTAAGTTTGGAAAAGGACGGGAAAGTGGCCCTGAATTCTTCTACGATCCCAAGTATGCTTTACGCCTTTGCCTTAAAGAAAAGCGAATGCGTGCCTGCGTTCATATATACAGTATGATGTCCATGCATGAAGAAGCAGTTGCTCTCGCCCTACAGGTTGATCCTGAGCTTGCTATGGCTGAAGCTGACAAGGTTGAAGACGATGAGGACTTGAGAAAGAAGCTCTGGCTCATGGTTGCGAAGCATGTAATAGAACAGGAGAAGGGAGCTAAGAGGGAGAACATACGGAAGGCAATAGCGTTTCTTAAAGAAACAGATGGCCTTTTAAAGATAGAGGATATATTACCATTCTTTCCAGACTTTGCCCTGATTGATGACTTTAAGGAGGCAATTTGCTCATCATTAGAGGATTACAATAATCAAATCGAACAACTGAAGCAAGAGATGAATGATGCGACACATGGTGCCGACAACATCAGAAATGACATTAGTGCCCTTGCTCAAAGATATGCTGTGATTGATCGTGATGAAGAATGTGGGGTGTGTCGGCGTAAAATTTTAACTGTTGGTAGGGAGTATCAGATGACTAGGGGCTATTCATCAGTAGGGCAAATGGCCCCCTTTTATGTCTTTCCTTGTGGACATGCCTTCCATGCACAATGCCTGATTGCCCATGTTACACGTAGCACAAATGAAGCTCAAGCAGAGTATATTCTGGATCTGCAGAAGCAACTCAGTCTATTGGACGGAGAAGCTAGGAAGGATTCAAATGGGCCCCTGACTGACGAGACCATAACAAGCATGGCCCCTGTAGACAAGCTCCGATCACAGTTGGATGATGCTGTGGCCAGCGAATGCCCATTTTGTGGGGACTTAATGATCCGCGAGATCTCTCTGCCTTTCATTCTTCCTGAGGAACAGTATCAGAGTACTTCATGGGATATACAGTCGCGTAATCTTGGAAACCAGAGGAGTCTTTCTTTGACTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006904 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007275 GO:0007610 GO:0007632 GO:0007634 GO:0008104 GO:0008150 GO:0008333 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009653 GO:0009705 GO:0009790 GO:0009987 GO:0010015 GO:0010256 GO:0010876 GO:0012505 GO:0015711 GO:0015718 GO:0015721 GO:0015849 GO:0015850 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0021700 GO:0022406 GO:0022622 GO:0030154 GO:0030674 GO:0030897 GO:0030903 GO:0031090 GO:0031338 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032879 GO:0032940 GO:0032991 GO:0033036 GO:0033043 GO:0033059 GO:0033263 GO:0035542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043473 GO:0043476 GO:0043482 GO:0043485 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045176 GO:0046903 GO:0046907 GO:0046942 GO:0048066 GO:0048069 GO:0048278 GO:0048364 GO:0048468 GO:0048513 GO:0048568 GO:0048731 GO:0048757 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0050931 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0051716 GO:0060034 GO:0060035 GO:0060036 GO:0060090 GO:0060627 GO:0065007 GO:0071702 GO:0071840 GO:0080171 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099402 GO:0140029 GO:0140056 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin Pep3_Vps18

Protein Analysis

987

Amino Acids

111.91

Weight (kDa)

5.17

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pep3_Vps18 PF05131 18 - 360 6.9e-82 Pep3/Vps18/deep orange beta-propeller domain
Clathrin PF00637 611 - 744 8e-08 Region in Clathrin and VPS
VPS18_RING_C PF26148 865 - 952 1.6e-06 VPS18 RING C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12470
fragaria_vesca FvH4_6g44560 FvH4_6g44560
malus_domestica MD14G1065300.v1.1 MD17G1084700.v1.1
prunus_persica Prupe.3G231000_v2.0.a1
pyrus_communis pycom17g08190
rosa_chinensis RchiOBHm_Chr2g0161771
rosa_laevigata RLG00000021298
rosa_multiflora Rmu_sc0003662.1_g000015
rosa_roxburghii Rroxscaffold_2G00088620
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2BG571500 Rh2DG580400
rosa_wichuraiana Rw2G046160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 7
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 4 cut(s) 257, 266, 865, 2435
AccB7I CCANNNNNTGG 1 cut(s) 2837
AccI GTMKAC 1 cut(s) 2784
AccII CGCG 5 cut(s) 53, 63, 307, 2856, 2923
AciI CCGC 9 cut(s) 13, 51, 63, 83, 179, 305, 756, 863, 2854
AclWI GGATC 5 cut(s) 215, 2012, 2126, 2697, 2845
AcoI YGGCCR 6 cut(s) 84, 315, 412, 879, 1761, 2817
AcsI RAATTY 6 cut(s) 763, 802, 890, 1462, 2006, 2526
AcuI CTGAAG 8 cut(s) 876, 950, 1148, 1304, 1619, 1682, 2172, 2426
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 2435
AfaI GTAC 7 cut(s) 47, 259, 403, 717, 1188, 1406, 2902
AfiI CCNNNNNNNGG 1 cut(s) 2837
AflIII ACRYGT 1 cut(s) 2651
AhdI GACNNNNNGTC 1 cut(s) 65
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 3 cut(s) 223, 350, 1897
AjuI GAANNNNNNNTTGG 2 cut(s) 1643, 1675
Alw21I GWGCWC 3 cut(s) 108, 1626, 1954
Alw26I GTCTC 2 cut(s) 1540, 2753
Alw44I GTGCAC 2 cut(s) 1622, 1950
AlwI GGATC 5 cut(s) 215, 2012, 2126, 2697, 2845
Ama87I CYCGRG 1 cut(s) 188
ApaI GGGCCC 1 cut(s) 2748
ApaLI GTGCAC 2 cut(s) 1622, 1950
ApeKI GCWGC 4 cut(s) 1133, 1136, 1199, 1229
ApoI RAATTY 6 cut(s) 763, 802, 890, 1462, 2006, 2526
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 257
AspA2I CCTAGG 1 cut(s) 1781
AspLEI GCGC 1 cut(s) 1809
AspS9I GGNCC 8 cut(s) 31, 1171, 1894, 2000, 2587, 2744, 2745, 2776
AsuC2I CCSGG 4 cut(s) 189, 190, 262, 663
AsuHPI GGTGA 3 cut(s) 349, 677, 1868
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 3 cut(s) 31, 1171, 1894
AvrII CCTAGG 1 cut(s) 1781
AxyI CCTNAGG 1 cut(s) 2883
BaeGI GKGCMC 5 cut(s) 870, 1626, 1954, 2467, 2748
BalI TGGCCA 4 cut(s) 317, 414, 1763, 2819
BanI GGYRCC 4 cut(s) 257, 266, 865, 2435
BanII GRGCYC 1 cut(s) 2748
BbsI GAAGAC 1 cut(s) 2175
Bbv12I GWGCWC 3 cut(s) 108, 1626, 1954
BbvCI CCTCAGC 1 cut(s) 1194
BbvI GCAGC 4 cut(s) 1120, 1123, 1186, 1241
BccI CCATC 5 cut(s) 117, 511, 553, 1753, 2282
BceAI ACGGC 1 cut(s) 866
BciT130I CCWGG 3 cut(s) 225, 352, 1899
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 4 cut(s) 189, 190, 262, 663
BcoDI GTCTC 2 cut(s) 1540, 2753
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 8 cut(s) 38, 114, 546, 624, 1593, 1782, 2559, 2727
BfmI CTRYAG 3 cut(s) 2123, 2693, 2781
BfuAI ACCTGC 1 cut(s) 7
BglII AGATCT 1 cut(s) 2859
BisI GCNGC 8 cut(s) 14, 51, 84, 305, 1134, 1137, 1200, 1230
BlnI CCTAGG 1 cut(s) 1781
BlsI GCNGC 8 cut(s) 15, 52, 85, 306, 1135, 1138, 1201, 1231
BmcAI AGTACT 1 cut(s) 2902
Bme1390I CCNGG 7 cut(s) 189, 190, 225, 262, 352, 663, 1899
Bme18I GGWCC 3 cut(s) 31, 1171, 1894
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 8 cut(s) 31, 1171, 1894, 2000, 2587, 2744, 2745, 2776
BmrFI CCNGG 7 cut(s) 189, 190, 225, 262, 352, 663, 1899
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 8 cut(s) 601, 646, 1012, 1241, 1723, 1794, 2413, 2800
BmuI ACTGGG 1 cut(s) 864
BpiI GAAGAC 1 cut(s) 2175
BpmI CTGGAG 4 cut(s) 246, 756, 813, 1920
Bpu10I CCTNAGC 2 cut(s) 1194, 2136
BpuEI CTTGAG 2 cut(s) 2203, 2655
BpuMI CCSGG 4 cut(s) 189, 190, 262, 663
BsaAI YACGTR 1 cut(s) 2654
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 2753
BsaJI CCNNGG 6 cut(s) 188, 189, 261, 1515, 1781, 1897
BsaWI WCCGGW 1 cut(s) 640
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 1 cut(s) 2837
Bse1I ACTGG 2 cut(s) 796, 870
Bse21I CCTNAGG 1 cut(s) 2883
Bse3DI GCAATG 2 cut(s) 249, 1806
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 3 cut(s) 225, 352, 1899
BseDI CCNNGG 6 cut(s) 188, 189, 261, 1515, 1781, 1897
BseGI GGATG 9 cut(s) 128, 442, 616, 637, 1363, 1525, 1534, 1764, 2812
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 2837
BseMI GCAATG 2 cut(s) 249, 1806
BseMII CTCAG 8 cut(s) 788, 837, 1185, 1237, 1496, 2127, 2719, 2874
BseNI ACTGG 2 cut(s) 796, 870
BseRI GAGGAG 2 cut(s) 1286, 2956
BseSI GKGCMC 5 cut(s) 870, 1626, 1954, 2467, 2748
BseX3I CGGCCG 1 cut(s) 84
BseXI GCAGC 4 cut(s) 1120, 1123, 1186, 1241
Bsh1236I CGCG 5 cut(s) 53, 63, 307, 2856, 2923
Bsh1285I CGRYCG 1 cut(s) 87
BshNI GGYRCC 4 cut(s) 257, 266, 865, 2435
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 3 cut(s) 108, 1626, 1954
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 6 cut(s) 87, 133, 189, 261, 641, 663
BslFI GGGAC 4 cut(s) 72, 171, 1184, 2855
BslI CCNNNNNNNGG 1 cut(s) 2837
BsmAI GTCTC 2 cut(s) 1540, 2753
BsmFI GGGAC 4 cut(s) 72, 171, 1184, 2855
BsmI GAATGC 4 cut(s) 196, 346, 2064, 2831
Bso31I GGTCTC 1 cut(s) 2753
BsoBI CYCGRG 1 cut(s) 188
Bsp120I GGGCCC 1 cut(s) 2744
Bsp1286I GDGCHC 6 cut(s) 108, 870, 1626, 1954, 2467, 2748
BspACI CCGC 9 cut(s) 13, 51, 63, 83, 179, 305, 756, 863, 2854
BspCNI CTCAG 8 cut(s) 787, 838, 1186, 1236, 1497, 2128, 2718, 2875
BspFNI CGCG 5 cut(s) 53, 63, 307, 2856, 2923
BspHI TCATGA 1 cut(s) 504
BspMAI CTGCAG 1 cut(s) 2697
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 5 cut(s) 215, 2012, 2126, 2697, 2845
BspQI GCTCTTC 1 cut(s) 828
BspT107I GGYRCC 4 cut(s) 257, 266, 865, 2435
BspTNI GGTCTC 1 cut(s) 2753
BsrDI GCAATG 2 cut(s) 249, 1806
BsrI ACTGG 2 cut(s) 796, 870
BssECI CCNNGG 6 cut(s) 188, 189, 261, 1515, 1781, 1897
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 1515, 1781
Bst2UI CCWGG 3 cut(s) 225, 352, 1899
Bst6I CTCTTC 2 cut(s) 828, 1569
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 643
BstBAI YACGTR 1 cut(s) 2654
BstC8I GCNNGC 5 cut(s) 302, 2064, 2068, 2142, 2821
BstDEI CTNAG 9 cut(s) 774, 846, 1194, 1223, 1505, 2136, 2241, 2705, 2883
BstF5I GGATG 9 cut(s) 128, 442, 616, 637, 1363, 1525, 1534, 1764, 2812
BstFNI CGCG 5 cut(s) 53, 63, 307, 2856, 2923
BstHHI GCGC 1 cut(s) 1809
BstMAI GTCTC 2 cut(s) 1540, 2753
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 9 cut(s) 251, 620, 643, 827, 1142, 1199, 1655, 1742, 2624
BstNI CCWGG 3 cut(s) 225, 352, 1899
BstNSI RCATGY 3 cut(s) 1829, 2219, 2618
BstSCI CCNGG 7 cut(s) 187, 188, 223, 260, 350, 661, 1897
BstSFI CTRYAG 3 cut(s) 2123, 2693, 2781
BstSLI GKGCMC 5 cut(s) 870, 1626, 1954, 2467, 2748
BstUI CGCG 5 cut(s) 53, 63, 307, 2856, 2923
BstV1I GCAGC 4 cut(s) 1120, 1123, 1186, 1241
BstV2I GAAGAC 1 cut(s) 2175
BstX2I RGATCY 2 cut(s) 2689, 2859
BstXI CCANNNNNNTGG 1 cut(s) 877
BstYI RGATCY 2 cut(s) 2689, 2859
BstZI CGGCCG 1 cut(s) 84
Bsu36I CCTNAGG 1 cut(s) 2883
BtsCI GGATG 9 cut(s) 128, 442, 616, 637, 1363, 1525, 1534, 1764, 2812
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 4 cut(s) 235, 685, 1293, 1443
BveI ACCTGC 1 cut(s) 7
Cac8I GCNNGC 5 cut(s) 302, 2064, 2068, 2142, 2821
CciI TCATGA 1 cut(s) 504
CfoI GCGC 1 cut(s) 1809
Cfr13I GGNCC 8 cut(s) 31, 1171, 1894, 2000, 2587, 2744, 2745, 2776
Cfr9I CCCGGG 1 cut(s) 188
CseI GACGC 3 cut(s) 69, 296, 1319
Csp6I GTAC 7 cut(s) 46, 258, 402, 716, 1187, 1405, 2901
CviQI GTAC 7 cut(s) 46, 258, 402, 716, 1187, 1405, 2901
DdeI CTNAG 9 cut(s) 774, 846, 1194, 1223, 1505, 2136, 2241, 2705, 2883
DraI TTTAAA 2 cut(s) 762, 2298
DraIII CACNNNGTG 1 cut(s) 2435
DriI GACNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 6 cut(s) 84, 315, 412, 879, 1761, 2817
EagI CGGCCG 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 828, 1569
Eam1105I GACNNNNNGTC 1 cut(s) 65
EarI CTCTTC 2 cut(s) 828, 1569
EclXI CGGCCG 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 1515, 1781
Eco24I GRGCYC 1 cut(s) 2748
Eco31I GGTCTC 1 cut(s) 2753
Eco47I GGWCC 3 cut(s) 31, 1171, 1894
Eco52I CGGCCG 1 cut(s) 84
Eco57I CTGAAG 8 cut(s) 876, 950, 1148, 1304, 1619, 1682, 2172, 2426
Eco81I CCTNAGG 1 cut(s) 2883
Eco88I CYCGRG 1 cut(s) 188
EcoO109I RGGNCCY 2 cut(s) 1894, 2745
EcoRI GAATTC 2 cut(s) 1462, 2006
EcoRII CCWGG 3 cut(s) 223, 350, 1897
EcoT14I CCWWGG 2 cut(s) 1515, 1781
EcoT22I ATGCAT 2 cut(s) 1738, 2100
EcoT38I GRGCYC 1 cut(s) 2748
ErhI CCWWGG 2 cut(s) 1515, 1781
FaqI GGGAC 4 cut(s) 72, 171, 1184, 2855
FauI CCCGC 1 cut(s) 172
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 2784
Fnu4HI GCNGC 8 cut(s) 14, 51, 84, 305, 1134, 1137, 1200, 1230
FokI GGATG 9 cut(s) 135, 449, 623, 624, 1370, 1532, 1541, 1771, 2819
FriOI GRGCYC 1 cut(s) 2748
Fsp4HI GCNGC 8 cut(s) 14, 51, 84, 305, 1134, 1137, 1200, 1230
FspBI CTAG 8 cut(s) 38, 114, 546, 624, 1593, 1782, 2559, 2727
GlaI GCGC 1 cut(s) 1808
GluI GCNGC 8 cut(s) 14, 51, 84, 305, 1134, 1137, 1200, 1230
GsuI CTGGAG 4 cut(s) 246, 756, 813, 1920
HapII CCGG 6 cut(s) 87, 133, 189, 261, 641, 663
HgaI GACGC 3 cut(s) 69, 296, 1319
HhaI GCGC 1 cut(s) 1809
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 1 cut(s) 1807
HinP1I GCGC 1 cut(s) 1807
HincII GTYRAC 1 cut(s) 1300
HindII GTYRAC 1 cut(s) 1300
HpaII CCGG 6 cut(s) 87, 133, 189, 261, 641, 663
HphI GGTGA 3 cut(s) 349, 677, 1868
Hpy166II GTNNAC 9 cut(s) 31, 1300, 1624, 1820, 1876, 1906, 1952, 2612, 2785
Hpy8I GTNNAC 9 cut(s) 31, 1300, 1624, 1820, 1876, 1906, 1952, 2612, 2785
Hpy99I CGWCG 1 cut(s) 98
HpyCH4IV ACGT 3 cut(s) 96, 1388, 2653
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 620, 643, 827, 1142, 1199, 1655, 1742, 2624
HpyF3I CTNAG 9 cut(s) 774, 846, 1194, 1223, 1505, 2136, 2241, 2705, 2883
HpySE526I ACGT 3 cut(s) 96, 1388, 2653
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 1 cut(s) 1807
KpnI GGTACC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 973
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 8 cut(s) 193, 231, 297, 634, 737, 1717, 2237, 2796
Lsp1109I GCAGC 4 cut(s) 1120, 1123, 1186, 1241
LweI GCATC 8 cut(s) 601, 646, 1012, 1241, 1723, 1794, 2413, 2800
MaeI CTAG 8 cut(s) 38, 114, 546, 624, 1593, 1782, 2559, 2727
MaeII ACGT 3 cut(s) 96, 1388, 2653
MaeIII GTNAC 6 cut(s) 565, 626, 1154, 1329, 1508, 2647
MflI RGATCY 2 cut(s) 2689, 2859
MhlI GDGCHC 6 cut(s) 108, 870, 1626, 1954, 2467, 2748
MlsI TGGCCA 4 cut(s) 317, 414, 1763, 2819
MluNI TGGCCA 4 cut(s) 317, 414, 1763, 2819
MlyI GAGTC 5 cut(s) 75, 573, 1505, 1760, 2953
MmeI TCCRAC 2 cut(s) 588, 2784
Mox20I TGGCCA 4 cut(s) 317, 414, 1763, 2819
Mph1103I ATGCAT 2 cut(s) 1738, 2100
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 4 cut(s) 317, 414, 1763, 2819
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 4 cut(s) 317, 414, 1763, 2819
MspA1I CMGCKG 1 cut(s) 1031
MspI CCGG 6 cut(s) 87, 133, 189, 261, 641, 663
MspR9I CCNGG 7 cut(s) 189, 190, 225, 262, 352, 663, 1899
Mva1269I GAATGC 4 cut(s) 196, 346, 2064, 2831
MvaI CCWGG 3 cut(s) 225, 352, 1899
MvnI CGCG 5 cut(s) 53, 63, 307, 2856, 2923
MwoI GCNNNNNNNGC 9 cut(s) 251, 620, 643, 827, 1142, 1199, 1655, 1742, 2624
NciI CCSGG 4 cut(s) 189, 190, 262, 663
NmeAIII GCCGAG 1 cut(s) 1118
NmuCI GTSAC 2 cut(s) 1154, 1508
NsiI ATGCAT 2 cut(s) 1738, 2100
NspI RCATGY 3 cut(s) 1829, 2219, 2618
PagI TCATGA 1 cut(s) 504
PciSI GCTCTTC 1 cut(s) 828
PctI GAATGC 4 cut(s) 196, 346, 2064, 2831
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 5 cut(s) 5, 155, 744, 986, 2735
PflMI CCANNNNNTGG 1 cut(s) 2837
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 8 cut(s) 15, 52, 85, 306, 1135, 1138, 1201, 1231
PleI GAGTC 5 cut(s) 74, 573, 1505, 1759, 2952
PpsI GAGTC 5 cut(s) 74, 573, 1505, 1759, 2952
Ppu21I YACGTR 1 cut(s) 2654
PpuMI RGGWCCY 1 cut(s) 1894
Psp5II RGGWCCY 1 cut(s) 1894
Psp6I CCWGG 3 cut(s) 223, 350, 1897
PspGI CCWGG 3 cut(s) 223, 350, 1897
PspOMI GGGCCC 1 cut(s) 2744
PspPI GGNCC 8 cut(s) 31, 1171, 1894, 2000, 2587, 2744, 2745, 2776
PspPPI RGGWCCY 1 cut(s) 1894
PstI CTGCAG 1 cut(s) 2697
PsuI RGATCY 2 cut(s) 2689, 2859
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 7 cut(s) 47, 259, 403, 717, 1188, 1406, 2902
RsaNI GTAC 7 cut(s) 46, 258, 402, 716, 1187, 1405, 2901
RseI CAYNNNNRTG 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 828
SatI GCNGC 8 cut(s) 14, 51, 84, 305, 1134, 1137, 1200, 1230
Sau96I GGNCC 8 cut(s) 31, 1171, 1894, 2000, 2587, 2744, 2745, 2776
ScaI AGTACT 1 cut(s) 2902
SchI GAGTC 5 cut(s) 75, 573, 1505, 1760, 2953
ScrFI CCNGG 7 cut(s) 189, 190, 225, 262, 352, 663, 1899
SduI GDGCHC 6 cut(s) 108, 870, 1626, 1954, 2467, 2748
SfaNI GCATC 8 cut(s) 601, 646, 1012, 1241, 1723, 1794, 2413, 2800
SfcI CTRYAG 3 cut(s) 2123, 2693, 2781
SinI GGWCC 3 cut(s) 31, 1171, 1894
SmaI CCCGGG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 2182, 2670
SmoI CTYRAG 2 cut(s) 2182, 2670
SpeI ACTAGT 1 cut(s) 113
SsiI CCGC 9 cut(s) 13, 51, 63, 83, 179, 305, 756, 863, 2854
SspMI CTAG 8 cut(s) 38, 114, 546, 624, 1593, 1782, 2559, 2727
StyD4I CCNGG 7 cut(s) 187, 188, 223, 260, 350, 661, 1897
StyI CCWWGG 2 cut(s) 1515, 1781
TaiI ACGT 3 cut(s) 99, 1391, 2656
TaqI TCGA 5 cut(s) 162, 1269, 1567, 1814, 2397
TatI WGTACW 4 cut(s) 715, 1186, 1404, 2900
TauI GCSGC 4 cut(s) 16, 53, 86, 307
TfiI GAWTC 5 cut(s) 5, 155, 744, 986, 2735
TscAI CASTG 4 cut(s) 242, 685, 1300, 1450
TseFI GTSAC 2 cut(s) 1154, 1508
TseI GCWGC 4 cut(s) 1133, 1136, 1199, 1229
Tsp45I GTSAC 2 cut(s) 1154, 1508
TspGWI ACGGA 3 cut(s) 1348, 2272, 2733
TspMI CCCGGG 1 cut(s) 188
TspRI CASTG 4 cut(s) 242, 685, 1300, 1450
Van91I CCANNNNNTGG 1 cut(s) 2837
VneI GTGCAC 2 cut(s) 1622, 1950
VpaK11BI GGWCC 3 cut(s) 31, 1171, 1894
XapI RAATTY 6 cut(s) 763, 802, 890, 1462, 2006, 2526
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 3 cut(s) 1829, 2219, 2618
XcmI CCANNNNNNNNNTGG 2 cut(s) 1522, 2770
XmaI CCCGGG 1 cut(s) 188
XmaJI CCTAGG 1 cut(s) 1781
XmiI GTMKAC 1 cut(s) 2784
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 8 cut(s) 38, 114, 546, 624, 1593, 1782, 2559, 2727
ZraI GACGTC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 2902
Zsp2I ATGCAT 2 cut(s) 1738, 2100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.