Prupe.3G231000_v2.0.a1

vacuolar protein sorting-associated protein 18 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0016149
Physical Location & Seq
Reverse (-)
22833080 .. 22843661
10582 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G231000.1

Sequence Viewer

Length: 2964 bp
ATGGATTCAGGTAGGCAGGTGTTCACGGTGGATCTTCTAGAAAGATATGCCGCCAAGGGGCGCGGAGTTATCACCTGTATGGCTGCCGGAAACGACGTCATTTTGTTGGGAACCAGTAAAGGTTGGATCATTCGACACGATTTTGGCCTCGGCGATTCATATGATATTGATCTCTCCGCGGGTCGTCCTGGGGAGCAATCCATCCATAGAGTTTTTGTTGATCCTGGAGGGAGCCACTGTATTGCCACTGTTGTTGGTAGTGGGGGTGCTGATACTTTCTATACTCATGCAAAATGGACCAAGCCTCGTATTTTAACCAAGCTCAAGGGTCTTGTTGTAAATGCCGTCGCCTGGAACAGACAACAGATAACAGAAGCTTCGACAAAGGAAGTCATTCTCGGTACAGACAATGGCCAACTTCATGAAATGGCTGTGGATGAGAAGGATAAGAAGGAGAAATATGTGAAGTTTCTTTTTGAACTATTAGAACTTCCAGAGGCTTTTATGAGTTTGCAGATGGAAACTGGGACCATTCTCAATGGAACTAGATACTATATAATGGCTGTAACTCCTACACGACTCTATTCTTTTACTGGTATTGGATTGCTGGAAACTGTTTTTGCTAGTTACTTAGATCATGTGGTGCATTTCATGGAACTTCCTGGTGAAATACCGAACAGTGAACTGCATTTCTATATCAAGCAAAGGAGAGCGGTACATTTTGCATGGCTTTCTGGTGCTGGTATCTATCACGGTGGTTTAAATTTTGGGGCACAACATAGTTCTCCTAATGGAGATGAAAATTTTGTGGAGAACAAGGCTCTTTTGAACTATTCAAGTTTGAGTGAGGGTGCTGAATTGGTGAAACCCAGTTCTATGACAGTTTCAGAATTTCATTTCTTGCTTCTTATCGGGAATAAGGTTAAGGTTGTAAACAGAATAAGTGAGCAAATTATTGAGGAACTTCAGTTTGATCAAACACCTGAGTCAGTTTCAAGGGGTGTCATTGGATTGTGCAGTGATGCCACTGCTGGGTTGTTCTATGCATATGATCAAAACTCTGTATTTCAGGTGTCTGTTAATGATGAAGGCCGAGACATGTGGAAAGTCTACCTGGACATGAAAGAGTATGCTGCTGCTCTGGCAAATTGTCGTGACCCACTTCAGCGGGACCAAGTATATTTGGTGCAGGCTGAAGCTGCATTTGCCTCCAAGGATTATCTTAGAGCAGCATCTTTCTATGCAAAGATTAATTATATATTATCATTCGAGGAGATCACTCTGAAGTTCATCACTGTAAATGAACAGGATGCTTTGAGGACCTTTCTATTGCGAAAACTTGATAGCCTTGCGAAGGATGACAAATGCCAAATAACAATGATTTCAACATGGGCAACTGAATTGTACTTGGATAAGATAAATCGGCTACTATTGGAAGATGACACTGCGTTAGATAATCGTAATTCAGAGTATCACTCGATCATGAAAGAATTTCGTGCATTTCTTAGTGACTGCAAGGATGTATTGGATGAGGCAACTACCATGAGACTTTTAGAAAGTTATGGTCGAGTTGAAGAGTTGGTGTTTTTCGCTAGTCTGAAGGAACTGCATGAAATTGTAGTTCACCATTATATTCAGCAAGGGGAGGCAAAGAAAGCATTGGAGGTGCTTCAAAAACCTTCTGTCCCAATAGATCTTCAGTACAAGTTTGCCCCGGACCTCATCATGCTAGATGCATATGAAGCTGTAGAGTCATGGATGGCAACAAACAACCTCAACCCAAGGAAACTCATTCCTGCAATGATGCGTTATTCAAGTGAACCACATGCAAGAAATGAAACACACGAAGTCATCAAATATTTGGAATACTGTGTTCATCGTTTGCATAACGAGGATCCTGGAGTTCACAACTTGCTATTATCGCTATATGCCAAGCAGGAAGATGACAGTGCACTTTTGCGTTTCCTACAATTCAAGTTTGGAAAAGGACGGGAAAATGGCCCAGAGTTCTTCTATGATCCCAAGTATGCTTTACGCCTTTGCCTCAAGGAAAAGCGAATGCGTGCCTGTGTTCATATATACAGTATGATGTCCATGCATGAAGAAGCAGTTGCTCTTGCCCTACAGGTTGATCCTGAGCTTGCTATGGCTGAAGCTGACAAGGTTGAAGACGATGAGGACTTGAGAAAGAAGCTTTGGCTCATGGTTGCGAAGCATGTTATAGAACAGGAAAAGGGAGCTAAAAGGGAGAACATACGAAAGGCAATAGCATTTCTTAAAGAAACTGATGGCCTTTTAAAGATAGAGGATATATTACCATTCTTTCCTGATTTTGCCCTGATTGATGACTTCAAGGAGGCAATTTGCTCATCATTAGAGGATTACAACAATCAAATCGAACTACTGAAGCAGGAGATGAATGATGCCACACATGGTGCAGATAACATTAGAAACGACATTAGTGCACTGGCTCAGAGATATGCTGTGATTGATCGTGATGAAGAATGTGGGGTTTGTCAGCGTAAGATTTTAACTGTGCGTAAGGAGTATCAGTTGGCTAGGGGCTATACATCAGTTGGCCAAATGGCCCCATTTTATGTCTTTCCATGTGGACATGCCTTCCATGCGGAATGCCTGATTGCCCATGTCACACGTAGTACAAATGAATCTCAAGCAGAGTATATACTGGATCTGCAGAAGCAGCTTACTCTACTTGACGGGGAAGCTAGGAAGGATACAAATGGTAGCCTAACTGAGGAAACCATAACAAGCATGGCGCCCGTAGATAAGCTCCGATCACAGTTGGACGATGCCGTCGCCAGCGAATGCCCATTTTGTGGGGACCTAATGATCCGTGAGATCTCTCTGCCTTTCATCCTGCCAGAGGAGCAACAGCAGAATAATTCATGGGAGATAAATTCACGTAATCTTGGAAACCAGAGGAGCCTGTCTTTAAGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006904 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007275 GO:0007610 GO:0007632 GO:0007634 GO:0008104 GO:0008150 GO:0008333 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009653 GO:0009705 GO:0009790 GO:0009987 GO:0010015 GO:0010256 GO:0010876 GO:0012505 GO:0015711 GO:0015718 GO:0015721 GO:0015849 GO:0015850 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0021700 GO:0022406 GO:0022622 GO:0030154 GO:0030674 GO:0030897 GO:0030903 GO:0031090 GO:0031338 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032879 GO:0032940 GO:0032991 GO:0033036 GO:0033043 GO:0033059 GO:0033263 GO:0035542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043473 GO:0043476 GO:0043482 GO:0043485 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045176 GO:0046903 GO:0046907 GO:0046942 GO:0048066 GO:0048069 GO:0048278 GO:0048364 GO:0048468 GO:0048513 GO:0048568 GO:0048731 GO:0048757 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0050931 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0051716 GO:0060034 GO:0060035 GO:0060036 GO:0060090 GO:0060627 GO:0065007 GO:0071702 GO:0071840 GO:0080171 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099402 GO:0140029 GO:0140056 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin Pep3_Vps18

Protein Analysis

988

Amino Acids

112.06

Weight (kDa)

5.22

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12470
fragaria_vesca FvH4_6g44560 FvH4_6g44560
malus_domestica MD14G1065300.v1.1 MD17G1084700.v1.1
prunus_persica Prupe.3G231000_v2.0.a1
pyrus_communis pycom17g08190
rosa_chinensis RchiOBHm_Chr2g0161771
rosa_laevigata RLG00000021298
rosa_multiflora Rmu_sc0003662.1_g000015
rosa_roxburghii Rroxscaffold_2G00088620
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2BG571500 Rh2DG580400
rosa_wichuraiana Rw2G046160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 7
AasI GACNNNNNNGTC 1 cut(s) 2813
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 7
AccB1I GGYRCC 1 cut(s) 2776
AccB7I CCANNNNNTGG 1 cut(s) 2837
AccBSI CCGCTC 1 cut(s) 713
AccI GTMKAC 1 cut(s) 1110
AccII CGCG 2 cut(s) 63, 179
AciI CCGC 7 cut(s) 51, 63, 177, 179, 713, 1168, 2627
AclWI GGATC 9 cut(s) 39, 134, 215, 1889, 1902, 2012, 2126, 2697, 2845
AcoI YGGCCR 2 cut(s) 412, 2578
AcsI RAATTY 5 cut(s) 763, 802, 890, 1490, 2917
AcuI CTGAAG 8 cut(s) 950, 1148, 1215, 1304, 1619, 1682, 2172, 2426
AcyI GRCGYC 2 cut(s) 96, 2777
AdeI CACNNNGTG 1 cut(s) 2435
AfaI GTAC 5 cut(s) 403, 717, 1406, 1703, 2659
AfiI CCNNNNNNNGG 7 cut(s) 57, 351, 1032, 1354, 2755, 2837, 2884
AflIII ACRYGT 2 cut(s) 1098, 2651
AjnI CCWGG 6 cut(s) 187, 223, 350, 661, 1113, 1897
AjuI GAANNNNNNNTTGG 2 cut(s) 2179, 2211
Alw21I GWGCWC 2 cut(s) 1954, 2467
Alw26I GTCTC 2 cut(s) 1089, 1540
Alw44I GTGCAC 2 cut(s) 1950, 2463
AlwI GGATC 9 cut(s) 39, 134, 215, 1889, 1902, 2012, 2126, 2697, 2845
AoxI GGCC 7 cut(s) 145, 412, 1090, 1999, 2290, 2578, 2586
ApaLI GTGCAC 2 cut(s) 1950, 2463
ApeKI GCWGC 6 cut(s) 83, 1133, 1136, 1199, 1229, 2701
ApoI RAATTY 5 cut(s) 763, 802, 890, 1490, 2917
AseI ATTAAT 1 cut(s) 1251
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 2 cut(s) 63, 2779
AspS9I GGNCC 8 cut(s) 297, 528, 1171, 1320, 1717, 2000, 2587, 2842
AsuC2I CCSGG 1 cut(s) 1715
AsuHPI GGTGA 4 cut(s) 64, 677, 874, 1616
AvaII GGWCC 6 cut(s) 297, 528, 1171, 1320, 1717, 2842
BaeGI GKGCMC 3 cut(s) 775, 1954, 2467
BalI TGGCCA 2 cut(s) 414, 2580
BamHI GGATCC 1 cut(s) 1894
BanI GGYRCC 1 cut(s) 2776
BbsI GAAGAC 1 cut(s) 2175
Bbv12I GWGCWC 2 cut(s) 1954, 2467
BbvI GCAGC 6 cut(s) 70, 1120, 1123, 1186, 1241, 2713
BccI CCATC 4 cut(s) 209, 511, 1753, 2282
BceAI ACGGC 2 cut(s) 329, 2798
BcgI CGANNNNNNTGC 2 cut(s) 2444, 2478
BciT130I CCWGG 6 cut(s) 189, 225, 352, 663, 1115, 1899
BciVI GTATCC 1 cut(s) 2728
BclI TGATCA 2 cut(s) 973, 1051
BcnI CCSGG 1 cut(s) 1715
BcoDI GTCTC 2 cut(s) 1089, 1540
BfaI CTAG 7 cut(s) 38, 546, 624, 1593, 1730, 2559, 2727
BfmI CTRYAG 3 cut(s) 1746, 2123, 2693
BfoI RGCGCY 1 cut(s) 2780
BfuAI ACCTGC 1 cut(s) 7
BfuI GTATCC 1 cut(s) 2728
BglII AGATCT 2 cut(s) 1693, 2859
BisI GCNGC 7 cut(s) 51, 84, 1134, 1137, 1200, 1230, 2702
BlsI GCNGC 7 cut(s) 52, 85, 1135, 1138, 1201, 1231, 2703
Bme1390I CCNGG 7 cut(s) 189, 225, 352, 663, 1115, 1715, 1899
Bme18I GGWCC 6 cut(s) 297, 528, 1171, 1320, 1717, 2842
BmgT120I GGNCC 8 cut(s) 297, 528, 1171, 1320, 1717, 2000, 2587, 2842
BmiI GGNNCC 9 cut(s) 112, 233, 529, 1172, 1896, 2589, 2778, 2843, 2945
BmrFI CCNGG 7 cut(s) 189, 225, 352, 663, 1115, 1715, 1899
BmrI ACTGGG 2 cut(s) 534, 864
BmsI GCATC 7 cut(s) 1012, 1241, 1300, 1723, 1794, 2413, 2800
BmuI ACTGGG 2 cut(s) 534, 864
BpiI GAAGAC 1 cut(s) 2175
BplI GAGNNNNNCTC 2 cut(s) 1460, 1492
BpmI CTGGAG 2 cut(s) 246, 1920
Bpu10I CCTNAGC 1 cut(s) 2136
BpuEI CTTGAG 4 cut(s) 308, 2030, 2203, 2655
BpuMI CCSGG 1 cut(s) 1715
BsaAI YACGTR 2 cut(s) 2654, 2924
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 2 cut(s) 96, 2777
BsaJI CCNNGG 7 cut(s) 54, 148, 177, 188, 1212, 1713, 1781
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 2537, 2567
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 351, 1032, 1354, 2755, 2837, 2884
Bse1I ACTGG 6 cut(s) 114, 529, 598, 870, 2472, 2691
Bse3DI GCAATG 1 cut(s) 1806
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 6 cut(s) 189, 225, 352, 663, 1115, 1899
BseDI CCNNGG 7 cut(s) 54, 148, 177, 188, 1212, 1713, 1781
BseGI GGATG 8 cut(s) 201, 442, 1315, 1363, 1525, 1534, 1764, 2874
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 7 cut(s) 57, 351, 1032, 1354, 2755, 2837, 2884
BseMI GCAATG 1 cut(s) 1806
BseMII CTCAG 4 cut(s) 975, 2127, 2486, 2745
BseNI ACTGG 6 cut(s) 114, 529, 598, 870, 2472, 2691
BseRI GAGGAG 3 cut(s) 1286, 2900, 2956
BseSI GKGCMC 3 cut(s) 775, 1954, 2467
BseXI GCAGC 6 cut(s) 70, 1120, 1123, 1186, 1241, 2713
BseYI CCCAGC 1 cut(s) 1031
BsgI GTGCAG 3 cut(s) 1036, 1208, 2457
Bsh1236I CGCG 2 cut(s) 63, 179
BshFI GGCC 7 cut(s) 147, 414, 1092, 2001, 2292, 2580, 2588
BshNI GGYRCC 1 cut(s) 2776
BsiHKAI GWGCWC 2 cut(s) 1954, 2467
BsiSI CCGG 2 cut(s) 87, 1715
BslFI GGGAC 4 cut(s) 541, 1184, 1670, 2855
BslI CCNNNNNNNGG 7 cut(s) 57, 351, 1032, 1354, 2755, 2837, 2884
BsmAI GTCTC 2 cut(s) 1089, 1540
BsmFI GGGAC 4 cut(s) 541, 1184, 1670, 2855
BsmI GAATGC 3 cut(s) 2064, 2636, 2831
BsnI GGCC 7 cut(s) 147, 414, 1092, 2001, 2292, 2580, 2588
Bsp1286I GDGCHC 3 cut(s) 775, 1954, 2467
BspACI CCGC 7 cut(s) 51, 63, 177, 179, 713, 1168, 2627
BspANI GGCC 7 cut(s) 147, 414, 1092, 2001, 2292, 2580, 2588
BspCNI CTCAG 4 cut(s) 976, 2128, 2485, 2746
BspFNI CGCG 2 cut(s) 63, 179
BspHI TCATGA 2 cut(s) 421, 1482
BspLI GGNNCC 9 cut(s) 112, 233, 529, 1172, 1896, 2589, 2778, 2843, 2945
BspMAI CTGCAG 1 cut(s) 2697
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 9 cut(s) 39, 134, 215, 1889, 1902, 2012, 2126, 2697, 2845
BspT107I GGYRCC 1 cut(s) 2776
BsrBI CCGCTC 1 cut(s) 713
BsrDI GCAATG 1 cut(s) 1806
BsrI ACTGG 6 cut(s) 114, 529, 598, 870, 2472, 2691
BssECI CCNNGG 7 cut(s) 54, 148, 177, 188, 1212, 1713, 1781
BssNI GRCGYC 2 cut(s) 96, 2777
BssT1I CCWWGG 3 cut(s) 54, 1212, 1781
Bst2UI CCWGG 6 cut(s) 189, 225, 352, 663, 1115, 1899
Bst6I CTCTTC 1 cut(s) 1569
BstACI GRCGYC 2 cut(s) 96, 2777
BstBAI YACGTR 2 cut(s) 2654, 2924
BstC8I GCNNGC 4 cut(s) 1191, 2064, 2142, 2821
BstDEI CTNAG 7 cut(s) 631, 984, 1223, 1505, 2136, 2472, 2754
BstDSI CCRYGG 1 cut(s) 177
BstENI CCTNNNNNAGG 3 cut(s) 1352, 2753, 2882
BstF5I GGATG 8 cut(s) 201, 442, 1315, 1363, 1525, 1534, 1764, 2874
BstFNI CGCG 2 cut(s) 63, 179
BstH2I RGCGCY 1 cut(s) 2780
BstHHI GCGC 2 cut(s) 63, 2779
BstMAI GTCTC 2 cut(s) 1089, 1540
BstMWI GCNNNNNNNGC 9 cut(s) 1142, 1199, 1205, 1655, 1742, 1921, 2624, 2701, 2887
BstNI CCWGG 6 cut(s) 189, 225, 352, 663, 1115, 1899
BstNSI RCATGY 4 cut(s) 1102, 1829, 2219, 2618
BstSCI CCNGG 7 cut(s) 187, 223, 350, 661, 1113, 1713, 1897
BstSFI CTRYAG 3 cut(s) 1746, 2123, 2693
BstSLI GKGCMC 3 cut(s) 775, 1954, 2467
BstUI CGCG 2 cut(s) 63, 179
BstV1I GCAGC 6 cut(s) 70, 1120, 1123, 1186, 1241, 2713
BstV2I GAAGAC 1 cut(s) 2175
BstX2I RGATCY 5 cut(s) 31, 1693, 1894, 2689, 2859
BstYI RGATCY 5 cut(s) 31, 1693, 1894, 2689, 2859
BsuI GTATCC 1 cut(s) 2728
BsuRI GGCC 7 cut(s) 147, 414, 1092, 2001, 2292, 2580, 2588
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 8 cut(s) 201, 442, 1315, 1363, 1525, 1534, 1764, 2874
BtsI GCAGTG 3 cut(s) 1024, 1026, 1443
BtsIMutI CAGTG 9 cut(s) 235, 246, 685, 1024, 1026, 1293, 1443, 1954, 2465
BveI ACCTGC 1 cut(s) 7
Cac8I GCNNGC 4 cut(s) 1191, 2064, 2142, 2821
CciI TCATGA 2 cut(s) 421, 1482
CfoI GCGC 2 cut(s) 63, 2779
Cfr13I GGNCC 8 cut(s) 297, 528, 1171, 1320, 1717, 2000, 2587, 2842
Cfr42I CCGCGG 1 cut(s) 180
Csp6I GTAC 5 cut(s) 402, 716, 1405, 1702, 2658
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviQI GTAC 5 cut(s) 402, 716, 1405, 1702, 2658
DdeI CTNAG 7 cut(s) 631, 984, 1223, 1505, 2136, 2472, 2754
DinI GGCGCC 1 cut(s) 2778
DraI TTTAAA 2 cut(s) 762, 2298
DraIII CACNNNGTG 1 cut(s) 2435
DrdI GACNNNNNNGTC 1 cut(s) 2813
DseDI GACNNNNNNGTC 1 cut(s) 2813
EaeI YGGCCR 2 cut(s) 412, 2578
Eam1104I CTCTTC 1 cut(s) 1569
EarI CTCTTC 1 cut(s) 1569
Eco130I CCWWGG 3 cut(s) 54, 1212, 1781
Eco47I GGWCC 6 cut(s) 297, 528, 1171, 1320, 1717, 2842
Eco57I CTGAAG 8 cut(s) 950, 1148, 1215, 1304, 1619, 1682, 2172, 2426
EcoNI CCTNNNNNAGG 3 cut(s) 1352, 2753, 2882
EcoO109I RGGNCCY 2 cut(s) 1320, 2842
EcoRII CCWGG 6 cut(s) 187, 223, 350, 661, 1113, 1897
EcoT14I CCWWGG 3 cut(s) 54, 1212, 1781
EcoT22I ATGCAT 3 cut(s) 1048, 1738, 2100
EgeI GGCGCC 1 cut(s) 2778
EheI GGCGCC 1 cut(s) 2778
ErhI CCWWGG 3 cut(s) 54, 1212, 1781
FaqI GGGAC 4 cut(s) 541, 1184, 1670, 2855
FauI CCCGC 2 cut(s) 172, 1161
FauNDI CATATG 3 cut(s) 160, 1048, 1738
FbaI TGATCA 2 cut(s) 973, 1051
FblI GTMKAC 1 cut(s) 1110
Fnu4HI GCNGC 7 cut(s) 51, 84, 1134, 1137, 1200, 1230, 2702
FokI GGATG 8 cut(s) 188, 449, 1322, 1370, 1532, 1541, 1771, 2861
Fsp4HI GCNGC 7 cut(s) 51, 84, 1134, 1137, 1200, 1230, 2702
FspBI CTAG 7 cut(s) 38, 546, 624, 1593, 1730, 2559, 2727
GlaI GCGC 2 cut(s) 62, 2778
GluI GCNGC 7 cut(s) 51, 84, 1134, 1137, 1200, 1230, 2702
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 2 cut(s) 246, 1920
HaeII RGCGCY 1 cut(s) 2780
HaeIII GGCC 7 cut(s) 147, 414, 1092, 2001, 2292, 2580, 2588
HapII CCGG 2 cut(s) 87, 1715
HhaI GCGC 2 cut(s) 63, 2779
Hin1I GRCGYC 2 cut(s) 96, 2777
Hin6I GCGC 2 cut(s) 61, 2777
HinP1I GCGC 2 cut(s) 61, 2777
HindIII AAGCTT 2 cut(s) 375, 2192
HinfI GANTC 6 cut(s) 5, 155, 579, 986, 1751, 2666
HpaII CCGG 2 cut(s) 87, 1715
HphI GGTGA 4 cut(s) 64, 677, 874, 1616
Hpy188I TCNGA 6 cut(s) 889, 1284, 1468, 1599, 2475, 2795
Hpy188III TCNNGA 9 cut(s) 38, 422, 494, 913, 1154, 1483, 2135, 2327, 2495
Hpy99I CGWCG 3 cut(s) 98, 350, 2819
HpyAV CCTTC 8 cut(s) 436, 445, 1082, 1348, 1594, 1689, 2629, 2725
HpyCH4IV ACGT 3 cut(s) 96, 2653, 2923
HpyF10VI GCNNNNNNNGC 9 cut(s) 1142, 1199, 1205, 1655, 1742, 1921, 2624, 2701, 2887
HpyF3I CTNAG 7 cut(s) 631, 984, 1223, 1505, 2136, 2472, 2754
HpySE526I ACGT 3 cut(s) 96, 2653, 2923
Hsp92I GRCGYC 2 cut(s) 96, 2777
HspAI GCGC 2 cut(s) 61, 2777
KasI GGCGCC 1 cut(s) 2776
Ksp22I TGATCA 2 cut(s) 973, 1051
KspI CCGCGG 1 cut(s) 180
LmnI GCTCC 6 cut(s) 193, 231, 2237, 2796, 2887, 2943
Lsp1109I GCAGC 6 cut(s) 70, 1120, 1123, 1186, 1241, 2713
LweI GCATC 7 cut(s) 1012, 1241, 1300, 1723, 1794, 2413, 2800
MaeI CTAG 7 cut(s) 38, 546, 624, 1593, 1730, 2559, 2727
MaeII ACGT 3 cut(s) 96, 2653, 2923
MaeIII GTNAC 5 cut(s) 565, 626, 1154, 1508, 2647
MbiI CCGCTC 1 cut(s) 713
MboII GAAGA 9 cut(s) 26, 1448, 1586, 1688, 1952, 2002, 2114, 2180, 2513
MflI RGATCY 5 cut(s) 31, 1693, 1894, 2689, 2859
MhlI GDGCHC 3 cut(s) 775, 1954, 2467
MlsI TGGCCA 2 cut(s) 414, 2580
MluNI TGGCCA 2 cut(s) 414, 2580
Mly113I GGCGCC 1 cut(s) 2777
MlyI GAGTC 3 cut(s) 573, 995, 1760
MmeI TCCRAC 2 cut(s) 104, 2784
Mox20I TGGCCA 2 cut(s) 414, 2580
Mph1103I ATGCAT 3 cut(s) 1048, 1738, 2100
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 2 cut(s) 414, 2580
MseI TTAA 9 cut(s) 314, 761, 924, 1080, 1251, 2277, 2297, 2531, 2954
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 2 cut(s) 414, 2580
MspA1I CMGCKG 2 cut(s) 179, 1168
MspI CCGG 2 cut(s) 87, 1715
MspR9I CCNGG 7 cut(s) 189, 225, 352, 663, 1115, 1715, 1899
Mva1269I GAATGC 3 cut(s) 2064, 2636, 2831
MvaI CCWGG 6 cut(s) 189, 225, 352, 663, 1115, 1899
MvnI CGCG 2 cut(s) 63, 179
MwoI GCNNNNNNNGC 9 cut(s) 1142, 1199, 1205, 1655, 1742, 1921, 2624, 2701, 2887
NarI GGCGCC 1 cut(s) 2777
NciI CCSGG 1 cut(s) 1715
NdeI CATATG 3 cut(s) 160, 1048, 1738
NlaIV GGNNCC 9 cut(s) 112, 233, 529, 1172, 1896, 2589, 2778, 2843, 2945
NmeAIII GCCGAG 2 cut(s) 129, 1118
NmuCI GTSAC 3 cut(s) 1154, 1508, 2647
NsiI ATGCAT 3 cut(s) 1048, 1738, 2100
NspI RCATGY 4 cut(s) 1102, 1829, 2219, 2618
PagI TCATGA 2 cut(s) 421, 1482
PaqCI CACCTGC 1 cut(s) 7
PciI ACATGT 1 cut(s) 1098
PctI GAATGC 3 cut(s) 2064, 2636, 2831
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 5, 155, 2666
PflMI CCANNNNNTGG 1 cut(s) 2837
PfoI TCCNGGA 2 cut(s) 223, 1897
PkrI GCNGC 7 cut(s) 52, 85, 1135, 1138, 1201, 1231, 2703
PleI GAGTC 3 cut(s) 573, 994, 1759
PluTI GGCGCC 1 cut(s) 2780
PpsI GAGTC 3 cut(s) 573, 994, 1759
Ppu21I YACGTR 2 cut(s) 2654, 2924
PpuMI RGGWCCY 2 cut(s) 1320, 2842
PscI ACATGT 1 cut(s) 1098
PshBI ATTAAT 1 cut(s) 1251
Psp5II RGGWCCY 2 cut(s) 1320, 2842
Psp6I CCWGG 6 cut(s) 187, 223, 350, 661, 1113, 1897
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 6 cut(s) 187, 223, 350, 661, 1113, 1897
PspN4I GGNNCC 9 cut(s) 112, 233, 529, 1172, 1896, 2589, 2778, 2843, 2945
PspPI GGNCC 8 cut(s) 297, 528, 1171, 1320, 1717, 2000, 2587, 2842
PspPPI RGGWCCY 2 cut(s) 1320, 2842
PstI CTGCAG 1 cut(s) 2697
PsuI RGATCY 5 cut(s) 31, 1693, 1894, 2689, 2859
RsaI GTAC 5 cut(s) 403, 717, 1406, 1703, 2659
RsaNI GTAC 5 cut(s) 402, 716, 1405, 1702, 2658
RseI CAYNNNNRTG 1 cut(s) 77
SacII CCGCGG 1 cut(s) 180
SaqAI TTAA 9 cut(s) 314, 761, 924, 1080, 1251, 2277, 2297, 2531, 2954
SatI GCNGC 7 cut(s) 51, 84, 1134, 1137, 1200, 1230, 2702
Sau96I GGNCC 8 cut(s) 297, 528, 1171, 1320, 1717, 2000, 2587, 2842
SchI GAGTC 3 cut(s) 573, 995, 1760
ScrFI CCNGG 7 cut(s) 189, 225, 352, 663, 1115, 1715, 1899
SduI GDGCHC 3 cut(s) 775, 1954, 2467
SfaNI GCATC 7 cut(s) 1012, 1241, 1300, 1723, 1794, 2413, 2800
SfcI CTRYAG 3 cut(s) 1746, 2123, 2693
SfoI GGCGCC 1 cut(s) 2778
Sfr303I CCGCGG 1 cut(s) 180
SgrBI CCGCGG 1 cut(s) 180
SinI GGWCC 6 cut(s) 297, 528, 1171, 1320, 1717, 2842
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 4 cut(s) 323, 2045, 2182, 2670
SmoI CTYRAG 4 cut(s) 323, 2045, 2182, 2670
SsiI CCGC 7 cut(s) 51, 63, 177, 179, 713, 1168, 2627
SspDI GGCGCC 1 cut(s) 2776
SspI AATATT 1 cut(s) 1859
SspMI CTAG 7 cut(s) 38, 546, 624, 1593, 1730, 2559, 2727
StyD4I CCNGG 7 cut(s) 187, 223, 350, 661, 1113, 1713, 1897
StyI CCWWGG 3 cut(s) 54, 1212, 1781
TaiI ACGT 3 cut(s) 99, 2656, 2926
TaqI TCGA 6 cut(s) 133, 380, 1269, 1478, 1567, 2397
TatI WGTACW 3 cut(s) 1404, 1701, 2657
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 3 cut(s) 5, 155, 2666
Tru1I TTAA 9 cut(s) 314, 761, 924, 1080, 1251, 2277, 2297, 2531, 2954
Tru9I TTAA 9 cut(s) 314, 761, 924, 1080, 1251, 2277, 2297, 2531, 2954
TscAI CASTG 9 cut(s) 242, 253, 685, 1024, 1033, 1300, 1450, 1954, 2472
TseFI GTSAC 3 cut(s) 1154, 1508, 2647
TseI GCWGC 6 cut(s) 83, 1133, 1136, 1199, 1229, 2701
Tsp45I GTSAC 3 cut(s) 1154, 1508, 2647
TspGWI ACGGA 1 cut(s) 2843
TspRI CASTG 9 cut(s) 242, 253, 685, 1024, 1033, 1300, 1450, 1954, 2472
Van91I CCANNNNNTGG 1 cut(s) 2837
VneI GTGCAC 2 cut(s) 1950, 2463
VpaK11BI GGWCC 6 cut(s) 297, 528, 1171, 1320, 1717, 2842
VspI ATTAAT 1 cut(s) 1251
XagI CCTNNNNNAGG 3 cut(s) 1352, 2753, 2882
XapI RAATTY 5 cut(s) 763, 802, 890, 1490, 2917
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 4 cut(s) 1102, 1829, 2219, 2618
XcmI CCANNNNNNNNNTGG 1 cut(s) 2770
XmiI GTMKAC 1 cut(s) 1110
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 7 cut(s) 38, 546, 624, 1593, 1730, 2559, 2727
ZraI GACGTC 1 cut(s) 97
Zsp2I ATGCAT 3 cut(s) 1048, 1738, 2100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.