MD14G1065300.v1.1

vacuolar protein sorting-associated protein 18 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016149
Physical Location & Seq
Reverse (-)
6937003 .. 6952659
15657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1065300.v1.1.491

Sequence Viewer

Length: 2967 bp
ATGGATTCAGGGAGGCAGGTGTTCACGGTGGATCTTCTGGAACGATATGCTGCCAAGGGGCGCGGAAATATTACCTATATGGCTACTGGAAACGACGTCATTTTGCTCGGAACCAGTAAAGGTTGGATCATTCGACACGATTTCGGCGTCGGCGATTCGTTTGATATTGATCTTTCGGTGGGTCGTCCTGGGGAGCAATCCATCCATAGAGTTTTTGTTGATCCTGGAGGGAGCCACTGCATTGCCACCGTTGTTGGGAGTGGGGGTGCTGATACGTTCTATACTCATGCAAAATGGACCAAGCCCCGTCTTTTAACCAAGCTAAAAGGTCTTGTCGTGAATGCCGTTGCCTGGAACAGACAACAGATTACAGAAGCTTCAACAAAGGAAGTCATTCTCGGTACCGACAACGGCCAACTTTATGAAATGGCAGTCGACGAGAAGGATAAGAAGGAGAAATACCTAAAGTTTTTGTTCGAATTATTGGAACTTCCAGAGGCTTTCATGAGTTTGCAGATGGAAATGGCAACCATTCTCAGTGGGACTAGATACTATGTAATGGCTGTAACTCCTACAAGACTCTATTCTTTTACTGGTATTGGATCACTAGAAACTGTTTTTGCTGGTTACTCGGAACAAACAGTGCATTTCATGGAACTTCCTGGTGAAATTCCTAACAGTGAATTGCATTTCTATATCAAGCAAAGAAGAGCAATACATTTTGCATGGCTTTCTGGTGCTGGTATATATCATGGTGGTTTAAATTTTGGAGCACAACATAGTTCTCCTGATGGAGATGAAAATTTTGTGGAGAACAAGGCTCTTTTGAACTATTCAACTTTGAGTGAGGGTTCTGAAGCGGTGAAACCCAGTTCTATGGCTGTTTCAGAATTTCATTTCTTGCTTCTTATCGGGAATAGGGTTAAGGTTGTAAATAGAATAAGTGAGCAAACTATTGAGGAACTTCAGTTTGAGCAAACACCTGAAGCAGTTTCAAGGGGTGTCACTGGATTGTGTAGTGATGCCACTGCTGGGTTGTTCTATGCATATGATCAAAACTCTGTATTTCAGGTGTCTGTTAATGATGAAGGCCGAGACATGTGGAAAGTTTACCTGGACATGAAGGAGTACGCTGCTGCTTTGGCAAATTGCCGCGACCCACTTCAGAGGGACCAAGTATATTTGGTGCAGGTCGCTGAAGCTGCATTTGCCGCCAAGGATTATCTTAGAGCAGCATCATTCTATGCAAAAATTAATTACATATTGTCGTTCGAGGAGATCAGTTTGAAGTTTATCACTGTAAATGAACAGGATGCCTTAAGAACTTTTCTATTGCGGAAACTTGATAGCCTTGCGGTGGATGACAAATGCCAAGTAACAATGATTTCGACGTGGGCAACTGAACTGTACTTGGATAAGATAAATCGTCTACTATTGGAAGATGATACTGCAGTGGATAATCGTAATTCAGAGTACCACTTGATCATTAAAGAGTTTCGTGCATTTCTTAGTGACTCCAAGGTTGTTTTGGATGAGGCAACTACCATGAGACTTTTAGAAAGTTATGGCCGAGTCGAAGAGTTGGTTTTTTTTGCTAGTCTGAAGGAACAGTATGAAATTGTAGTCCACCATTATATTCAGCAAGGAGAGGCAAAGAAAGCATTGGAGGTGCTCCAAAAACCTACTGTTCCTATAGATCTTCAGTACAAGTTTGCACCAGACCTAATCATGCTTGATGCATATGAAGCTGTAGAGTCATGGATGACTACAAACAACCTCAATCCAAGGAAACTCATTCCTGCAATGATGCGTTATTCAAGTGAACCACATGCAAGAAATGAAACACACGAAGTCATCAAATATTTGGAATACTGTGTTCATCGTTTGCATAATGAGGATCCTGGTGTTCACAACTTGCTATTATCACTATATGCCAAACAGGAAGACGACAGTGCACTTTTGCGTTTCCTTCAATTCAAATTCGGAAAAGGACGGGAAATTGGCCCAGAGTTCTTCTATGATCCCAAGTATGCTTTACGCCTTTGCCTCAAGGAAAAGCGAATGCGTGCCTGTGTTCATATATATAGTATGATGTCCATGCATGAAGAAGCAGTTGCTCTTGCCCTGCAGGTTGATCCTGAGCTTGCTATGGCTGAGGCTGACAAGGTTGAAGATGATGAGGACCTGAGAAAGAAGCTTTGGCTCATGGTTGCGAAGCATGTTATAGAACAGGAAAAGGGAGCTAAAAGGGAGAACATTCGGAAGGCAATAGCATTTCTTAAAGAAACTGATGGCCTTTTAAAGATAGAGGATATATTACCATTCTTTCCGGACTTTGCCCTCATTGATGACTTCAAGGAGGCAATTTGCTCATCATTAGAGGATTACAACAATCAAATTGAAGAACTGAAGCAAGAGATGAATGATGCAACACATGGTGCCGATAACATTAGAAATGACATTAGTGCACTTGCTCAAAGATATGCTGTGATTGATCGTGATGAAGAATGCGGGGTTTGTCGGCGTAAGATTTTAACTGTGGGTAGGGAGTATCAGTTGGCTAGAGGCTATGCATCCGTAGGCCAAATGGCTCCATTCTATGTGTTTCCATGCGGACATGCCTTCCATGCCGAATGCCTGATTGCCCACGTCACACGTAGTACAAATGAAGCTCAAGCAGAGTATATACTGGATCTGCAGAAGCAGCTTACTCTACTTGACGGGGAAGCTAGGAAGGATTCAAATGGTAGCCTTACTGAGGAAACCATAACAAGCATGGCCCCCGTAGATAAGCTGCGGTCGCAGTTGGATGATGCAGTGGCAAGCGAATGCCCATTTTGTGGGGACCTAATGATCCGTGAGATCTCTCTACCTTTCATCCTTCCAGAGGAGCAACAGCAGAATACATCATGGGAGATAAAGTCACATAATCTTGGACACCAGAGGAGCCTGGGTTTATCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006904 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007275 GO:0007610 GO:0007632 GO:0007634 GO:0008104 GO:0008150 GO:0008333 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009653 GO:0009705 GO:0009790 GO:0009987 GO:0010015 GO:0010256 GO:0010876 GO:0012505 GO:0015711 GO:0015718 GO:0015721 GO:0015849 GO:0015850 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0021700 GO:0022406 GO:0022622 GO:0030154 GO:0030674 GO:0030897 GO:0030903 GO:0031090 GO:0031338 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032879 GO:0032940 GO:0032991 GO:0033036 GO:0033043 GO:0033059 GO:0033263 GO:0035542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043473 GO:0043476 GO:0043482 GO:0043485 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045176 GO:0046903 GO:0046907 GO:0046942 GO:0048066 GO:0048069 GO:0048278 GO:0048364 GO:0048468 GO:0048513 GO:0048568 GO:0048731 GO:0048757 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0050931 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0051716 GO:0060034 GO:0060035 GO:0060036 GO:0060090 GO:0060627 GO:0065007 GO:0071702 GO:0071840 GO:0080171 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099402 GO:0140029 GO:0140056 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin Pep3_Vps18

Protein Analysis

989

Amino Acids

112.07

Weight (kDa)

5.16

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pep3_Vps18 PF05131 16 - 360 1.7e-81 Pep3/Vps18/deep orange beta-propeller domain
Clathrin PF00637 608 - 745 3.3e-08 Region in Clathrin and VPS
VPS18_RING_C PF26148 866 - 953 9.3e-07 VPS18 RING C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12470
fragaria_vesca FvH4_6g44560 FvH4_6g44560
malus_domestica MD14G1065300.v1.1 MD17G1084700.v1.1
prunus_persica Prupe.3G231000_v2.0.a1
pyrus_communis pycom17g08190
rosa_chinensis RchiOBHm_Chr2g0161771
rosa_laevigata RLG00000021298
rosa_multiflora Rmu_sc0003662.1_g000015
rosa_roxburghii Rroxscaffold_2G00088620
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2BG571500 Rh2DG580400
rosa_wichuraiana Rw2G046160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 7
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 3 cut(s) 7, 1180, 2119
Acc65I GGTACC 1 cut(s) 401
AccB1I GGYRCC 2 cut(s) 401, 2438
AccB7I CCANNNNNTGG 1 cut(s) 2840
AccI GTMKAC 2 cut(s) 435, 1429
AccII CGCG 2 cut(s) 63, 1155
AccIII TCCGGA 1 cut(s) 2329
AciI CCGC 9 cut(s) 63, 860, 1153, 1212, 1336, 1355, 2511, 2613, 2797
AcoI YGGCCR 2 cut(s) 412, 1567
AcsI RAATTY 5 cut(s) 669, 763, 802, 890, 1979
AcuI CTGAAG 8 cut(s) 876, 950, 1005, 1148, 1218, 1622, 1685, 2429
AcyI GRCGYC 2 cut(s) 96, 147
AdeI CACNNNGTG 1 cut(s) 2438
AfaI GTAC 6 cut(s) 403, 1130, 1409, 1475, 1706, 2662
AfiI CCNNNNNNNGG 7 cut(s) 255, 351, 1032, 1357, 2758, 2840, 2887
AflII CTTAAG 1 cut(s) 1318
AflIII ACRYGT 2 cut(s) 1098, 2654
AjiI CACGTC 2 cut(s) 1392, 2650
AjnI CCWGG 7 cut(s) 187, 223, 350, 661, 1113, 1900, 2949
AjuI GAANNNNNNNTTGG 2 cut(s) 2182, 2214
Alw21I GWGCWC 4 cut(s) 775, 1674, 1957, 2470
Alw26I GTCTC 2 cut(s) 1089, 1543
Alw44I GTGCAC 2 cut(s) 1953, 2466
Aor13HI TCCGGA 1 cut(s) 2329
AoxI GGCC 7 cut(s) 412, 1090, 1567, 2002, 2293, 2581, 2778
ApaLI GTGCAC 2 cut(s) 1953, 2466
ApeKI GCWGC 7 cut(s) 50, 1133, 1136, 1202, 1232, 2704, 2794
ApoI RAATTY 5 cut(s) 669, 763, 802, 890, 1979
AseI ATTAAT 1 cut(s) 1254
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 401
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 6 cut(s) 297, 1171, 2003, 2182, 2779, 2845
AsuHPI GGTGA 2 cut(s) 677, 874
AsuII TTCGAA 1 cut(s) 477
AvaII GGWCC 4 cut(s) 297, 1171, 2182, 2845
BaeGI GKGCMC 2 cut(s) 1957, 2470
BaeI ACNNNNGTAYC 2 cut(s) 1437, 1470
BamHI GGATCC 1 cut(s) 1897
BanI GGYRCC 2 cut(s) 401, 2438
BarI GAAGNNNNNNTAC 2 cut(s) 443, 475
BbsI GAAGAC 1 cut(s) 1950
Bbv12I GWGCWC 4 cut(s) 775, 1674, 1957, 2470
BbvCI CCTCAGC 1 cut(s) 2154
BbvI GCAGC 7 cut(s) 37, 1120, 1123, 1189, 1244, 2716, 2781
BccI CCATC 4 cut(s) 209, 511, 785, 2285
BceAI ACGGC 2 cut(s) 329, 427
BciT130I CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
BclI TGATCA 2 cut(s) 1051, 1482
BcoDI GTCTC 2 cut(s) 1089, 1543
BfaI CTAG 5 cut(s) 546, 608, 1596, 2562, 2730
BfmI CTRYAG 5 cut(s) 1449, 1692, 1749, 2126, 2696
BfrI CTTAAG 1 cut(s) 1318
BfuAI ACCTGC 3 cut(s) 7, 1180, 2119
BglII AGATCT 2 cut(s) 1696, 2862
BisI GCNGC 9 cut(s) 51, 1134, 1137, 1153, 1203, 1212, 1233, 2705, 2795
BlsI GCNGC 9 cut(s) 52, 1135, 1138, 1154, 1204, 1213, 1234, 2706, 2796
Bme1390I CCNGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
Bme18I GGWCC 4 cut(s) 297, 1171, 2182, 2845
BmgBI CACGTC 2 cut(s) 1392, 2650
BmgT120I GGNCC 6 cut(s) 297, 1171, 2003, 2182, 2779, 2845
BmrFI CCNGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 8 cut(s) 1012, 1244, 1303, 1726, 1797, 2416, 2582, 2803
BmuI ACTGGG 1 cut(s) 864
BpiI GAAGAC 1 cut(s) 1950
BpmI CTGGAG 1 cut(s) 246
Bpu10I CCTNAGC 2 cut(s) 2139, 2154
Bpu14I TTCGAA 1 cut(s) 477
BpuEI CTTGAG 2 cut(s) 2033, 2658
BsaAI YACGTR 1 cut(s) 2657
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 2 cut(s) 96, 147
BsaJI CCNNGG 6 cut(s) 54, 188, 1215, 1518, 1784, 2950
BsaWI WCCGGW 1 cut(s) 2329
BsaXI ACNNNNNCTCC 7 cut(s) 250, 280, 2540, 2570, 2906, 2936, 2938
Bsc4I CCNNNNNNNGG 7 cut(s) 255, 351, 1032, 1357, 2758, 2840, 2887
Bse1I ACTGG 6 cut(s) 91, 114, 598, 870, 1012, 2694
Bse3DI GCAATG 2 cut(s) 240, 1809
Bse8I GATNNNNATC 1 cut(s) 168
BseAI TCCGGA 1 cut(s) 2329
BseBI CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
BseDI CCNNGG 6 cut(s) 54, 188, 1215, 1518, 1784, 2950
BseGI GGATG 8 cut(s) 201, 1318, 1366, 1537, 1767, 2573, 2815, 2877
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 7 cut(s) 255, 351, 1032, 1357, 2758, 2840, 2887
BseMI GCAATG 2 cut(s) 240, 1809
BseMII CTCAG 5 cut(s) 550, 2130, 2145, 2177, 2748
BseNI ACTGG 6 cut(s) 91, 114, 598, 870, 1012, 2694
BseRI GAGGAG 3 cut(s) 1289, 2903, 2959
BseSI GKGCMC 2 cut(s) 1957, 2470
BseXI GCAGC 7 cut(s) 37, 1120, 1123, 1189, 1244, 2716, 2781
BseYI CCCAGC 1 cut(s) 1031
BsgI GTGCAG 1 cut(s) 1208
Bsh1236I CGCG 2 cut(s) 63, 1155
Bsh1285I CGRYCG 1 cut(s) 2801
BshFI GGCC 7 cut(s) 414, 1092, 1569, 2004, 2295, 2583, 2780
BshNI GGYRCC 2 cut(s) 401, 2438
BsiEI CGRYCG 1 cut(s) 2801
BsiHKAI GWGCWC 4 cut(s) 775, 1674, 1957, 2470
BsiSI CCGG 1 cut(s) 2330
BslFI GGGAC 3 cut(s) 556, 1184, 2858
BslI CCNNNNNNNGG 7 cut(s) 255, 351, 1032, 1357, 2758, 2840, 2887
BsmAI GTCTC 2 cut(s) 1089, 1543
BsmFI GGGAC 3 cut(s) 556, 1184, 2858
BsmI GAATGC 5 cut(s) 346, 2067, 2513, 2639, 2834
BsnI GGCC 7 cut(s) 414, 1092, 1569, 2004, 2295, 2583, 2780
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 4 cut(s) 775, 1674, 1957, 2470
Bsp13I TCCGGA 1 cut(s) 2329
BspACI CCGC 9 cut(s) 63, 860, 1153, 1212, 1336, 1355, 2511, 2613, 2797
BspANI GGCC 7 cut(s) 414, 1092, 1569, 2004, 2295, 2583, 2780
BspCNI CTCAG 5 cut(s) 549, 2131, 2146, 2178, 2749
BspEI TCCGGA 1 cut(s) 2329
BspFNI CGCG 2 cut(s) 63, 1155
BspHI TCATGA 1 cut(s) 504
BspMAI CTGCAG 3 cut(s) 1453, 2130, 2700
BspMI ACCTGC 3 cut(s) 7, 1180, 2119
BspQI GCTCTTC 1 cut(s) 703
BspT104I TTCGAA 1 cut(s) 477
BspT107I GGYRCC 2 cut(s) 401, 2438
BspTI CTTAAG 1 cut(s) 1318
BsrDI GCAATG 2 cut(s) 240, 1809
BsrI ACTGG 6 cut(s) 91, 114, 598, 870, 1012, 2694
BssECI CCNNGG 6 cut(s) 54, 188, 1215, 1518, 1784, 2950
BssNI GRCGYC 2 cut(s) 96, 147
BssT1I CCWWGG 4 cut(s) 54, 1215, 1518, 1784
Bst2UI CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
Bst6I CTCTTC 2 cut(s) 703, 1572
BstACI GRCGYC 2 cut(s) 96, 147
BstAFI CTTAAG 1 cut(s) 1318
BstBAI YACGTR 1 cut(s) 2657
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 3 cut(s) 2067, 2145, 2824
BstDEI CTNAG 7 cut(s) 536, 1226, 1508, 2139, 2154, 2186, 2757
BstENI CCTNNNNNAGG 2 cut(s) 2756, 2885
BstF5I GGATG 8 cut(s) 201, 1318, 1366, 1537, 1767, 2573, 2815, 2877
BstFNI CGCG 2 cut(s) 63, 1155
BstHHI GCGC 1 cut(s) 63
BstMAI GTCTC 2 cut(s) 1089, 1543
BstMCI CGRYCG 1 cut(s) 2801
BstMWI GCNNNNNNNGC 9 cut(s) 1142, 1202, 1208, 1211, 1658, 1745, 2627, 2704, 2800
BstNI CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
BstNSI RCATGY 4 cut(s) 1102, 1832, 2222, 2621
BstSCI CCNGG 7 cut(s) 187, 223, 350, 661, 1113, 1900, 2949
BstSFI CTRYAG 5 cut(s) 1449, 1692, 1749, 2126, 2696
BstSLI GKGCMC 2 cut(s) 1957, 2470
BstUI CGCG 2 cut(s) 63, 1155
BstV1I GCAGC 7 cut(s) 37, 1120, 1123, 1189, 1244, 2716, 2781
BstV2I GAAGAC 1 cut(s) 1950
BstX2I RGATCY 5 cut(s) 31, 1696, 1897, 2692, 2862
BstXI CCANNNNNNTGG 1 cut(s) 877
BstYI RGATCY 5 cut(s) 31, 1696, 1897, 2692, 2862
BsuRI GGCC 7 cut(s) 414, 1092, 1569, 2004, 2295, 2583, 2780
BtrI CACGTC 2 cut(s) 1392, 2650
BtsCI GGATG 8 cut(s) 201, 1318, 1366, 1537, 1767, 2573, 2815, 2877
BtsI GCAGTG 4 cut(s) 235, 1026, 1458, 2823
BveI ACCTGC 3 cut(s) 7, 1180, 2119
Cac8I GCNNGC 3 cut(s) 2067, 2145, 2824
CciI TCATGA 1 cut(s) 504
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 6 cut(s) 297, 1171, 2003, 2182, 2779, 2845
CseI GACGC 1 cut(s) 136
Csp6I GTAC 6 cut(s) 402, 1129, 1408, 1474, 1705, 2661
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviQI GTAC 6 cut(s) 402, 1129, 1408, 1474, 1705, 2661
DdeI CTNAG 7 cut(s) 536, 1226, 1508, 2139, 2154, 2186, 2757
DraI TTTAAA 2 cut(s) 762, 2301
DraIII CACNNNGTG 1 cut(s) 2438
EaeI YGGCCR 2 cut(s) 412, 1567
Eam1104I CTCTTC 2 cut(s) 703, 1572
EarI CTCTTC 2 cut(s) 703, 1572
Eco130I CCWWGG 4 cut(s) 54, 1215, 1518, 1784
Eco47I GGWCC 4 cut(s) 297, 1171, 2182, 2845
Eco57I CTGAAG 8 cut(s) 876, 950, 1005, 1148, 1218, 1622, 1685, 2429
EcoNI CCTNNNNNAGG 2 cut(s) 2756, 2885
EcoO109I RGGNCCY 2 cut(s) 2182, 2845
EcoRII CCWGG 7 cut(s) 187, 223, 350, 661, 1113, 1900, 2949
EcoT14I CCWWGG 4 cut(s) 54, 1215, 1518, 1784
EcoT22I ATGCAT 4 cut(s) 1048, 1741, 2103, 2575
ErhI CCWWGG 4 cut(s) 54, 1215, 1518, 1784
FaqI GGGAC 3 cut(s) 556, 1184, 2858
FauI CCCGC 1 cut(s) 2504
FauNDI CATATG 2 cut(s) 1048, 1741
FbaI TGATCA 2 cut(s) 1051, 1482
FblI GTMKAC 2 cut(s) 435, 1429
Fnu4HI GCNGC 9 cut(s) 51, 1134, 1137, 1153, 1203, 1212, 1233, 2705, 2795
FokI GGATG 8 cut(s) 188, 1325, 1373, 1544, 1774, 2560, 2822, 2864
Fsp4HI GCNGC 9 cut(s) 51, 1134, 1137, 1153, 1203, 1212, 1233, 2705, 2795
FspBI CTAG 5 cut(s) 546, 608, 1596, 2562, 2730
GlaI GCGC 1 cut(s) 62
GluI GCNGC 9 cut(s) 51, 1134, 1137, 1153, 1203, 1212, 1233, 2705, 2795
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 7 cut(s) 414, 1092, 1569, 2004, 2295, 2583, 2780
HapII CCGG 1 cut(s) 2330
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 2 cut(s) 96, 147
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HindIII AAGCTT 2 cut(s) 375, 2195
HinfI GANTC 7 cut(s) 5, 155, 579, 1514, 1572, 1754, 2738
HpaII CCGG 1 cut(s) 2330
HphI GGTGA 2 cut(s) 677, 874
Hpy166II GTNNAC 9 cut(s) 24, 436, 1111, 1430, 1627, 1823, 1909, 1955, 2468
Hpy188I TCNGA 9 cut(s) 110, 634, 856, 889, 1167, 1471, 1602, 1985, 2262
Hpy8I GTNNAC 9 cut(s) 24, 436, 1111, 1430, 1627, 1823, 1909, 1955, 2468
Hpy99I CGWCG 4 cut(s) 98, 152, 440, 1393
HpyCH4IV ACGT 5 cut(s) 96, 275, 1391, 2649, 2656
HpyF10VI GCNNNNNNNGC 9 cut(s) 1142, 1202, 1208, 1211, 1658, 1745, 2627, 2704, 2800
HpyF3I CTNAG 7 cut(s) 536, 1226, 1508, 2139, 2154, 2186, 2757
HpySE526I ACGT 5 cut(s) 96, 275, 1391, 2649, 2656
Hsp92I GRCGYC 2 cut(s) 96, 147
HspAI GCGC 1 cut(s) 61
Kpn2I TCCGGA 1 cut(s) 2329
KpnI GGTACC 1 cut(s) 405
Ksp22I TGATCA 2 cut(s) 1051, 1482
LguI GCTCTTC 1 cut(s) 703
LmnI GCTCC 8 cut(s) 193, 231, 770, 1677, 2240, 2596, 2890, 2946
Lsp1109I GCAGC 7 cut(s) 37, 1120, 1123, 1189, 1244, 2716, 2781
LweI GCATC 8 cut(s) 1012, 1244, 1303, 1726, 1797, 2416, 2582, 2803
MaeI CTAG 5 cut(s) 546, 608, 1596, 2562, 2730
MaeII ACGT 5 cut(s) 96, 275, 1391, 2649, 2656
MaeIII GTNAC 7 cut(s) 565, 626, 1003, 1375, 1511, 2650, 2922
MflI RGATCY 5 cut(s) 31, 1696, 1897, 2692, 2862
MhlI GDGCHC 4 cut(s) 775, 1674, 1957, 2470
MlyI GAGTC 4 cut(s) 573, 1508, 1581, 1763
MmeI TCCRAC 2 cut(s) 104, 2787
Mph1103I ATGCAT 4 cut(s) 1048, 1741, 2103, 2575
MroI TCCGGA 1 cut(s) 2329
MroXI GAANNNNTTC 1 cut(s) 393
MspCI CTTAAG 1 cut(s) 1318
MspI CCGG 1 cut(s) 2330
MspR9I CCNGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
Mva1269I GAATGC 5 cut(s) 346, 2067, 2513, 2639, 2834
MvaI CCWGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
MvnI CGCG 2 cut(s) 63, 1155
MwoI GCNNNNNNNGC 9 cut(s) 1142, 1202, 1208, 1211, 1658, 1745, 2627, 2704, 2800
NdeI CATATG 2 cut(s) 1048, 1741
NmeAIII GCCGAG 2 cut(s) 1118, 1595
NmuCI GTSAC 4 cut(s) 1003, 1511, 2650, 2922
NsiI ATGCAT 4 cut(s) 1048, 1741, 2103, 2575
NspI RCATGY 4 cut(s) 1102, 1832, 2222, 2621
NspV TTCGAA 1 cut(s) 477
PagI TCATGA 1 cut(s) 504
PaqCI CACCTGC 1 cut(s) 7
PciI ACATGT 1 cut(s) 1098
PciSI GCTCTTC 1 cut(s) 703
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 150, 342
PctI GAATGC 5 cut(s) 346, 2067, 2513, 2639, 2834
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 5, 155, 2738
PflMI CCANNNNNTGG 1 cut(s) 2840
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 9 cut(s) 52, 1135, 1138, 1154, 1204, 1213, 1234, 2706, 2796
PleI GAGTC 4 cut(s) 573, 1508, 1580, 1762
PpsI GAGTC 4 cut(s) 573, 1508, 1580, 1762
Ppu21I YACGTR 1 cut(s) 2657
PpuMI RGGWCCY 2 cut(s) 2182, 2845
PscI ACATGT 1 cut(s) 1098
PshBI ATTAAT 1 cut(s) 1254
Psp5II RGGWCCY 2 cut(s) 2182, 2845
Psp6I CCWGG 7 cut(s) 187, 223, 350, 661, 1113, 1900, 2949
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 7 cut(s) 187, 223, 350, 661, 1113, 1900, 2949
PspPI GGNCC 6 cut(s) 297, 1171, 2003, 2182, 2779, 2845
PspPPI RGGWCCY 2 cut(s) 2182, 2845
PstI CTGCAG 3 cut(s) 1453, 2130, 2700
PsuI RGATCY 5 cut(s) 31, 1696, 1897, 2692, 2862
RsaI GTAC 6 cut(s) 403, 1130, 1409, 1475, 1706, 2662
RsaNI GTAC 6 cut(s) 402, 1129, 1408, 1474, 1705, 2661
SalI GTCGAC 1 cut(s) 434
SapI GCTCTTC 1 cut(s) 703
SatI GCNGC 9 cut(s) 51, 1134, 1137, 1153, 1203, 1212, 1233, 2705, 2795
Sau96I GGNCC 6 cut(s) 297, 1171, 2003, 2182, 2779, 2845
SbfI CCTGCAGG 1 cut(s) 2130
SchI GAGTC 4 cut(s) 573, 1508, 1581, 1763
ScrFI CCNGG 7 cut(s) 189, 225, 352, 663, 1115, 1902, 2951
SdaI CCTGCAGG 1 cut(s) 2130
SduI GDGCHC 4 cut(s) 775, 1674, 1957, 2470
SfaNI GCATC 8 cut(s) 1012, 1244, 1303, 1726, 1797, 2416, 2582, 2803
SfcI CTRYAG 5 cut(s) 1449, 1692, 1749, 2126, 2696
SfuI TTCGAA 1 cut(s) 477
SinI GGWCC 4 cut(s) 297, 1171, 2182, 2845
SmlI CTYRAG 3 cut(s) 1318, 2048, 2673
SmoI CTYRAG 3 cut(s) 1318, 2048, 2673
Sse8387I CCTGCAGG 1 cut(s) 2130
SsiI CCGC 9 cut(s) 63, 860, 1153, 1212, 1336, 1355, 2511, 2613, 2797
SspI AATATT 2 cut(s) 70, 1862
SspMI CTAG 5 cut(s) 546, 608, 1596, 2562, 2730
StyD4I CCNGG 7 cut(s) 187, 223, 350, 661, 1113, 1900, 2949
StyI CCWWGG 4 cut(s) 54, 1215, 1518, 1784
TaiI ACGT 5 cut(s) 99, 278, 1394, 2652, 2659
TaqI TCGA 6 cut(s) 133, 435, 477, 1272, 1388, 1575
TatI WGTACW 3 cut(s) 1407, 1704, 2660
TauI GCSGC 2 cut(s) 1155, 1214
TfiI GAWTC 3 cut(s) 5, 155, 2738
TseFI GTSAC 4 cut(s) 1003, 1511, 2650, 2922
TseI GCWGC 7 cut(s) 50, 1133, 1136, 1202, 1232, 2704, 2794
Tsp45I GTSAC 4 cut(s) 1003, 1511, 2650, 2922
TspGWI ACGGA 2 cut(s) 2566, 2846
Van91I CCANNNNNTGG 1 cut(s) 2840
Vha464I CTTAAG 1 cut(s) 1318
VneI GTGCAC 2 cut(s) 1953, 2466
VpaK11BI GGWCC 4 cut(s) 297, 1171, 2182, 2845
VspI ATTAAT 1 cut(s) 1254
XagI CCTNNNNNAGG 2 cut(s) 2756, 2885
XapI RAATTY 5 cut(s) 669, 763, 802, 890, 1979
XceI RCATGY 4 cut(s) 1102, 1832, 2222, 2621
XcmI CCANNNNNNNNNTGG 2 cut(s) 1525, 2773
XmiI GTMKAC 2 cut(s) 435, 1429
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 5 cut(s) 546, 608, 1596, 2562, 2730
ZraI GACGTC 1 cut(s) 97
Zsp2I ATGCAT 4 cut(s) 1048, 1741, 2103, 2575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.