FvH4_6g45730

Converts proline to delta-1-pyrroline-5-carboxylate

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
Physical Location & Seq
Forward (+)
35028784 .. 35031791
3008 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45730.t1

Sequence Viewer

Length: 1482 bp
ATGGCAAACCGTGTGATCCCTTCAGCACAGAGAATCCTCAAGACGCTACGACCTCTCAACTCGGCTTCCTCCACCACCATCTCCGCCCTTTCGCCGATTACCTTCCCAGAGAAGGCACAACAACACAACCCTGGATTAGCGGAACAGCTGCTGCTGCCCAAGTCTGACGCGTTGTTCAACCTTGACGACGTGAACAACAGGCTGTTTGAGTCGGTTCCTACGGCGAAGCTCATCCGTTCGGCGGCGAATCTGCACATGGTTGCGATGGAGCCGATGGTGGATTTGGGAATGTGGATGATGAAGTCGAAGCTTATGACGACGCCGGTGGTTAAAGATCTCATTTTGGGGTTTATAAGGTCTACGTTTTATGAGCATTTCTGCGCTGGCGAGGACGCCGTCGGCACCGGTAAGTCGATTCGGGCGCTAAACGAGGCGGGGCTGAGGGGCATGCTGGTCTACGCGTTAGAGTACGCCAGCGACAACGAGTCGTGTGATCGGAACTTGCAGGGCTTCTTGGACACCGTTGAATCCACCAAGTCGCTTCCTCGTTCTTCTGTGAGCTTCATTATACTGAAGATTACTGCGATTTGCCCCATGAAGCTTCTGGAGCGTGTTAGTGACTTGCTGAGATGGCAACAGAGAGACCCTTCTCTCAATCTTCCATGGAAGCATGAGACTCTACCCATTTTCTCGGAATCCAGCCCGCTCTATCATACGATGGAAAAACCAGAACCTTTAACTCCAGAAGAAGAGCGTGATCTGGAGTTGGTCCATCAAAGACTACAAAGGCTATCCGAAAAGTGCCTAGAAGCCAATGTCCCCTTATCCGTGGATGCAGAGTACACATCGATTCAACCTTCCATTGATTACTTGACATATGCTTCAGCAATCAAGCACAACAAAGATGACAACCCAATTGTTTACGGAACAATCCAAGCTTACTTGAAAGATGCAAAGGAAAGAATGTTGCTAGCTTCCGAGGCTGCAGACAAGATGGGCATTCCCATGGGGTTCAAAGTGGTGAGGGGTGCTTATATGTCTAGTGAAAGTAAAAAAGCTTCTGCCTTGGGCTATCCATCTCCCATTCACAACAGCATTGAGGAAACACACGCATGCTTCAACGAGTGTGCTTCTTACATGCTTGAGAAAATTGGAAATGGCTCCGGAGCTGTTGTTCTTGCAACCCATAACGTAGAATCAGGGAAACTGGCTGTGAAAAAAGCACATGAAATTGGGGTCAGCAAGGTGCACCAGAAGCTCGAATTTGCTCAACTGTATGGAATGGCAGAAGCACTTTCCTTTGGTCTAAAAAATGCAGGGTTTCAAGTCAGCAAGTATATGCCCTTCGGACCAATAGAATTAGTGATGCCATACCTTTTACGGAGGGCTGAAGAAAACCGAGGGCTTTTATCTGCCTCAACTCTAGACAGACACCTTACCAGGGAGGAGTTGAAAAGGAGACTCAAAGCAGCAATTTTCTAA

Protein Analysis

494

Amino Acids

54.93

Weight (kDa)

7.21

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_dh PF01619 137 - 469 3.8e-66 Proline dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 401
AccBSI CCGCTC 1 cut(s) 706
AccI GTMKAC 2 cut(s) 359, 456
AccII CGCG 2 cut(s) 170, 461
AccIII TCCGGA 1 cut(s) 1163
AciI CCGC 5 cut(s) 84, 140, 242, 434, 704
AclWI GGATC 1 cut(s) 10
AcsI RAATTY 1 cut(s) 1262
AcuI CTGAAG 4 cut(s) 6, 593, 867, 1410
AcyI GRCGYC 2 cut(s) 320, 393
AfaI GTAC 2 cut(s) 470, 842
AfiI CCNNNNNNNGG 3 cut(s) 112, 241, 1441
AflIII ACRYGT 2 cut(s) 168, 459
AgeI ACCGGT 1 cut(s) 404
AgsI TTSAA 8 cut(s) 178, 527, 854, 946, 1015, 1120, 1325, 1453
AhdI GACNNNNNGTC 1 cut(s) 484
AjiI CACGTC 1 cut(s) 190
AjnI CCWGG 2 cut(s) 130, 1439
AloI GAACNNNNNNTCC 2 cut(s) 1158, 1190
Alw21I GWGCWC 1 cut(s) 1251
Alw26I GTCTC 3 cut(s) 636, 668, 1453
Alw44I GTGCAC 1 cut(s) 1247
AlwI GGATC 1 cut(s) 10
AlwNI CAGNNNCTG 1 cut(s) 151
Aor13HI TCCGGA 1 cut(s) 1163
ApaLI GTGCAC 1 cut(s) 1247
ApeKI GCWGC 5 cut(s) 148, 151, 154, 983, 1469
ApoI RAATTY 1 cut(s) 1262
AsiGI ACCGGT 1 cut(s) 404
AspLEI GCGC 2 cut(s) 383, 424
AspS9I GGNCC 2 cut(s) 769, 1349
AsuHPI GGTGA 1 cut(s) 1033
AsuNHI GCTAGC 1 cut(s) 970
AvaII GGWCC 2 cut(s) 769, 1349
BaeGI GKGCMC 1 cut(s) 1251
BanI GGYRCC 1 cut(s) 401
BarI GAAGNNNNNNTAC 4 cut(s) 1042, 1074, 1328, 1360
Bbv12I GWGCWC 1 cut(s) 1251
BbvCI CCTCAGC 1 cut(s) 440
BbvI GCAGC 4 cut(s) 135, 138, 141, 970
BccI CCATC 8 cut(s) 86, 259, 268, 624, 712, 780, 988, 1084
BceAI ACGGC 2 cut(s) 237, 380
BciT130I CCWGG 2 cut(s) 132, 1441
BcoDI GTCTC 3 cut(s) 636, 668, 1453
BfaI CTAG 4 cut(s) 806, 971, 1041, 1424
BfmI CTRYAG 1 cut(s) 984
BfoI RGCGCY 1 cut(s) 425
BglII AGATCT 1 cut(s) 334
BisI GCNGC 6 cut(s) 149, 152, 155, 243, 984, 1470
BlsI GCNGC 6 cut(s) 150, 153, 156, 244, 985, 1471
Bme1390I CCNGG 2 cut(s) 132, 1441
Bme18I GGWCC 2 cut(s) 769, 1349
BmeRI GACNNNNNGTC 1 cut(s) 484
BmgBI CACGTC 1 cut(s) 190
BmgT120I GGNCC 2 cut(s) 769, 1349
BmiI GGNNCC 4 cut(s) 216, 270, 403, 1162
BmrFI CCNGG 2 cut(s) 132, 1441
BmsI GCATC 3 cut(s) 823, 940, 1356
BmtI GCTAGC 1 cut(s) 974
BpmI CTGGAG 3 cut(s) 626, 726, 782
Bpu10I CCTNAGC 1 cut(s) 440
BpuEI CTTGAG 2 cut(s) 23, 1163
Bsa29I ATCGAT 1 cut(s) 848
BsaHI GRCGYC 2 cut(s) 320, 393
BsaI GGTCTC 1 cut(s) 636
BsaJI CCNNGG 8 cut(s) 130, 662, 828, 978, 1005, 1065, 1399, 1440
BsaWI WCCGGW 2 cut(s) 404, 1163
BsaXI ACNNNNNCTCC 2 cut(s) 1158, 1188
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 241, 1441
Bse118I RCCGGY 2 cut(s) 322, 404
Bse1I ACTGG 1 cut(s) 1212
BseAI TCCGGA 1 cut(s) 1163
BseBI CCWGG 2 cut(s) 132, 1441
BseCI ATCGAT 1 cut(s) 848
BseDI CCNNGG 8 cut(s) 130, 662, 828, 978, 1005, 1065, 1399, 1440
BseGI GGATG 3 cut(s) 231, 300, 838
BseLI CCNNNNNNNGG 3 cut(s) 112, 241, 1441
BseMII CTCAG 2 cut(s) 431, 617
BseNI ACTGG 1 cut(s) 1212
BseRI GAGGAG 1 cut(s) 1460
BseSI GKGCMC 1 cut(s) 1251
BseXI GCAGC 4 cut(s) 135, 138, 141, 970
BsgI GTGCAG 1 cut(s) 236
Bsh1236I CGCG 2 cut(s) 170, 461
BshNI GGYRCC 1 cut(s) 401
BshTI ACCGGT 1 cut(s) 404
BshVI ATCGAT 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 1251
BsiSI CCGG 3 cut(s) 323, 405, 1164
BslFI GGGAC 1 cut(s) 803
BslI CCNNNNNNNGG 3 cut(s) 112, 241, 1441
BsmAI GTCTC 3 cut(s) 636, 668, 1453
BsmFI GGGAC 1 cut(s) 803
BsmI GAATGC 1 cut(s) 999
Bso31I GGTCTC 1 cut(s) 636
Bsp1286I GDGCHC 1 cut(s) 1251
Bsp13I TCCGGA 1 cut(s) 1163
Bsp143I GATC 4 cut(s) 15, 334, 493, 757
Bsp19I CCATGG 2 cut(s) 662, 1005
BspACI CCGC 5 cut(s) 84, 140, 242, 434, 704
BspCNI CTCAG 2 cut(s) 432, 618
BspDI ATCGAT 1 cut(s) 848
BspEI TCCGGA 1 cut(s) 1163
BspFNI CGCG 2 cut(s) 170, 461
BspLI GGNNCC 4 cut(s) 216, 270, 403, 1162
BspMAI CTGCAG 1 cut(s) 988
BspOI GCTAGC 1 cut(s) 974
BspPI GGATC 1 cut(s) 10
BspQI GCTCTTC 1 cut(s) 744
BspT107I GGYRCC 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 636
BsrBI CCGCTC 1 cut(s) 706
BsrFI RCCGGY 2 cut(s) 322, 404
BsrI ACTGG 1 cut(s) 1212
BssAI RCCGGY 2 cut(s) 322, 404
BssECI CCNNGG 8 cut(s) 130, 662, 828, 978, 1005, 1065, 1399, 1440
BssMI GATC 4 cut(s) 15, 334, 493, 757
BssNI GRCGYC 2 cut(s) 320, 393
BssT1I CCWWGG 3 cut(s) 662, 1005, 1065
Bst2UI CCWGG 2 cut(s) 132, 1441
Bst4CI ACNGT 3 cut(s) 11, 523, 1275
Bst6I CTCTTC 1 cut(s) 744
BstACI GRCGYC 2 cut(s) 320, 393
BstC8I GCNNGC 6 cut(s) 385, 449, 475, 704, 972, 1114
BstDEI CTNAG 2 cut(s) 440, 626
BstDSI CCRYGG 3 cut(s) 662, 828, 1005
BstF5I GGATG 3 cut(s) 231, 300, 838
BstFNI CGCG 2 cut(s) 170, 461
BstH2I RGCGCY 1 cut(s) 425
BstHHI GCGC 2 cut(s) 383, 424
BstKTI GATC 4 cut(s) 18, 337, 496, 760
BstMAI GTCTC 3 cut(s) 636, 668, 1453
BstMBI GATC 4 cut(s) 15, 334, 493, 757
BstMWI GCNNNNNNNGC 5 cut(s) 154, 607, 631, 980, 1255
BstNI CCWGG 2 cut(s) 132, 1441
BstNSI RCATGY 3 cut(s) 451, 1116, 1141
BstSCI CCNGG 2 cut(s) 130, 1439
BstSFI CTRYAG 1 cut(s) 984
BstSLI GKGCMC 1 cut(s) 1251
BstUI CGCG 2 cut(s) 170, 461
BstV1I GCAGC 4 cut(s) 135, 138, 141, 970
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 848
BsuTUI ATCGAT 1 cut(s) 848
BtgI CCRYGG 3 cut(s) 662, 828, 1005
BtgZI GCGATG 1 cut(s) 278
BtrI CACGTC 1 cut(s) 190
BtsCI GGATG 3 cut(s) 231, 300, 838
Cac8I GCNNGC 6 cut(s) 385, 449, 475, 704, 972, 1114
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 2 cut(s) 383, 424
Cfr10I RCCGGY 2 cut(s) 322, 404
Cfr13I GGNCC 2 cut(s) 769, 1349
ClaI ATCGAT 1 cut(s) 848
CseI GACGC 4 cut(s) 52, 176, 328, 401
Csp6I GTAC 2 cut(s) 469, 841
CspAI ACCGGT 1 cut(s) 404
CviAII CATG 9 cut(s) 256, 448, 595, 663, 671, 1006, 1113, 1138, 1226
CviQI GTAC 2 cut(s) 469, 841
DdeI CTNAG 2 cut(s) 440, 626
DpnI GATC 4 cut(s) 17, 336, 495, 759
DpnII GATC 4 cut(s) 15, 334, 493, 757
DriI GACNNNNNGTC 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 744
Eam1105I GACNNNNNGTC 1 cut(s) 484
EarI CTCTTC 1 cut(s) 744
EciI GGCGGA 1 cut(s) 73
Eco130I CCWWGG 3 cut(s) 662, 1005, 1065
Eco31I GGTCTC 1 cut(s) 636
Eco47I GGWCC 2 cut(s) 769, 1349
Eco57I CTGAAG 4 cut(s) 6, 593, 867, 1410
EcoRII CCWGG 2 cut(s) 130, 1439
EcoT14I CCWWGG 3 cut(s) 662, 1005, 1065
ErhI CCWWGG 3 cut(s) 662, 1005, 1065
FaeI CATG 9 cut(s) 259, 451, 598, 666, 674, 1009, 1116, 1141, 1229
FaqI GGGAC 1 cut(s) 803
FatI CATG 9 cut(s) 255, 447, 594, 662, 670, 1005, 1112, 1137, 1225
FauI CCCGC 2 cut(s) 427, 711
FauNDI CATATG 1 cut(s) 877
FblI GTMKAC 2 cut(s) 359, 456
Fnu4HI GCNGC 6 cut(s) 149, 152, 155, 243, 984, 1470
FokI GGATG 3 cut(s) 218, 307, 845
Fsp4HI GCNGC 6 cut(s) 149, 152, 155, 243, 984, 1470
FspBI CTAG 4 cut(s) 806, 971, 1041, 1424
GlaI GCGC 2 cut(s) 382, 423
GluI GCNGC 6 cut(s) 149, 152, 155, 243, 984, 1470
GsuI CTGGAG 3 cut(s) 626, 726, 782
HaeII RGCGCY 1 cut(s) 425
HapII CCGG 3 cut(s) 323, 405, 1164
HgaI GACGC 4 cut(s) 52, 176, 328, 401
HhaI GCGC 2 cut(s) 383, 424
Hin1I GRCGYC 2 cut(s) 320, 393
Hin1II CATG 9 cut(s) 259, 451, 598, 666, 674, 1009, 1116, 1141, 1229
Hin6I GCGC 2 cut(s) 381, 422
HinP1I GCGC 2 cut(s) 381, 422
HindIII AAGCTT 4 cut(s) 308, 599, 936, 1056
HpaII CCGG 3 cut(s) 323, 405, 1164
HphI GGTGA 1 cut(s) 1033
Hpy166II GTNNAC 6 cut(s) 193, 360, 457, 843, 922, 1249
Hpy188I TCNGA 6 cut(s) 166, 498, 694, 796, 979, 1349
Hpy188III TCNNGA 6 cut(s) 40, 605, 743, 761, 1164, 1424
Hpy8I GTNNAC 6 cut(s) 193, 360, 457, 843, 922, 1249
Hpy99I CGWCG 3 cut(s) 191, 322, 401
HpyAV CCTTC 6 cut(s) 30, 106, 112, 657, 867, 1354
HpyCH4III ACNGT 3 cut(s) 11, 523, 1275
HpyCH4IV ACGT 3 cut(s) 189, 362, 1191
HpyCH4V TGCA 8 cut(s) 253, 505, 836, 953, 986, 1181, 1249, 1316
HpyF10VI GCNNNNNNNGC 5 cut(s) 154, 607, 631, 980, 1255
HpyF3I CTNAG 2 cut(s) 440, 626
HpySE526I ACGT 3 cut(s) 189, 362, 1191
Hsp92I GRCGYC 2 cut(s) 320, 393
Hsp92II CATG 9 cut(s) 259, 451, 598, 666, 674, 1009, 1116, 1141, 1229
HspAI GCGC 2 cut(s) 381, 422
Kpn2I TCCGGA 1 cut(s) 1163
Kzo9I GATC 4 cut(s) 15, 334, 493, 757
LguI GCTCTTC 1 cut(s) 744
LmnI GCTCC 4 cut(s) 268, 607, 1166, 1166
Lsp1109I GCAGC 4 cut(s) 135, 138, 141, 970
LweI GCATC 3 cut(s) 823, 940, 1356
MaeI CTAG 4 cut(s) 806, 971, 1041, 1424
MaeII ACGT 3 cut(s) 189, 362, 1191
MaeIII GTNAC 1 cut(s) 617
MalI GATC 4 cut(s) 17, 336, 495, 759
MbiI CCGCTC 1 cut(s) 706
MboI GATC 4 cut(s) 15, 334, 493, 757
MboII GAAGA 6 cut(s) 543, 586, 650, 758, 761, 1403
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 1251
MluCI AATT 6 cut(s) 915, 1149, 1230, 1262, 1358, 1473
MluI ACGCGT 2 cut(s) 168, 459
MlyI GAGTC 4 cut(s) 218, 494, 670, 1455
MroI TCCGGA 1 cut(s) 1163
MseI TTAA 2 cut(s) 330, 737
MslI CAYNNNNRTG 2 cut(s) 1004, 1111
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 3 cut(s) 323, 405, 1164
MspR9I CCNGG 2 cut(s) 132, 1441
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 999
MvaI CCWGG 2 cut(s) 132, 1441
MvnI CGCG 2 cut(s) 170, 461
MwoI GCNNNNNNNGC 5 cut(s) 154, 607, 631, 980, 1255
NcoI CCATGG 2 cut(s) 662, 1005
NdeI CATATG 1 cut(s) 877
NdeII GATC 4 cut(s) 15, 334, 493, 757
NheI GCTAGC 1 cut(s) 970
NlaIII CATG 9 cut(s) 259, 451, 598, 666, 674, 1009, 1116, 1141, 1229
NlaIV GGNNCC 4 cut(s) 216, 270, 403, 1162
NmeAIII GCCGAG 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 617
NspI RCATGY 3 cut(s) 451, 1116, 1141
PaeI GCATGC 2 cut(s) 451, 1116
PciSI GCTCTTC 1 cut(s) 744
PctI GAATGC 1 cut(s) 999
PfeI GAWTC 7 cut(s) 33, 247, 415, 527, 695, 850, 1196
PflFI GACNNNGTC 1 cut(s) 395
PinAI ACCGGT 1 cut(s) 404
PkrI GCNGC 6 cut(s) 150, 153, 156, 244, 985, 1471
PleI GAGTC 4 cut(s) 217, 493, 670, 1455
PpsI GAGTC 4 cut(s) 217, 493, 670, 1455
PsiI TTATAA 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 130, 1439
PspGI CCWGG 2 cut(s) 130, 1439
PspN4I GGNNCC 4 cut(s) 216, 270, 403, 1162
PspPI GGNCC 2 cut(s) 769, 1349
PstI CTGCAG 1 cut(s) 988
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 2 cut(s) 470, 842
RsaNI GTAC 2 cut(s) 469, 841
RseI CAYNNNNRTG 2 cut(s) 1004, 1111
SapI GCTCTTC 1 cut(s) 744
SaqAI TTAA 2 cut(s) 330, 737
SatI GCNGC 6 cut(s) 149, 152, 155, 243, 984, 1470
Sau3AI GATC 4 cut(s) 15, 334, 493, 757
Sau96I GGNCC 2 cut(s) 769, 1349
SchI GAGTC 4 cut(s) 218, 494, 670, 1455
ScrFI CCNGG 2 cut(s) 132, 1441
SduI GDGCHC 1 cut(s) 1251
SfaNI GCATC 3 cut(s) 823, 940, 1356
SfcI CTRYAG 1 cut(s) 984
SgrAI CRCCGGYG 1 cut(s) 322
SinI GGWCC 2 cut(s) 769, 1349
SmiMI CAYNNNNRTG 2 cut(s) 1004, 1111
SmlI CTYRAG 2 cut(s) 38, 1142
SmoI CTYRAG 2 cut(s) 38, 1142
SphI GCATGC 2 cut(s) 451, 1116
Sse9I AATT 6 cut(s) 915, 1149, 1230, 1262, 1358, 1473
SsiI CCGC 5 cut(s) 84, 140, 242, 434, 704
SspMI CTAG 4 cut(s) 806, 971, 1041, 1424
StyD4I CCNGG 2 cut(s) 130, 1439
StyI CCWWGG 3 cut(s) 662, 1005, 1065
TaaI ACNGT 3 cut(s) 11, 523, 1275
TaiI ACGT 3 cut(s) 192, 365, 1194
TaqI TCGA 4 cut(s) 305, 413, 848, 1260
TasI AATT 6 cut(s) 915, 1149, 1230, 1262, 1358, 1473
TatI WGTACW 1 cut(s) 840
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 7 cut(s) 33, 247, 415, 527, 695, 850, 1196
Tru1I TTAA 2 cut(s) 330, 737
Tru9I TTAA 2 cut(s) 330, 737
TseFI GTSAC 1 cut(s) 617
TseI GCWGC 5 cut(s) 148, 151, 154, 983, 1469
Tsp45I GTSAC 1 cut(s) 617
TspDTI ATGAA 4 cut(s) 314, 553, 611, 1242
TspGWI ACGGA 4 cut(s) 224, 817, 939, 1396
Tth111I GACNNNGTC 1 cut(s) 395
VneI GTGCAC 1 cut(s) 1247
VpaK11BI GGWCC 2 cut(s) 769, 1349
XapI RAATTY 1 cut(s) 1262
XbaI TCTAGA 1 cut(s) 1423
XceI RCATGY 3 cut(s) 451, 1116, 1141
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmiI GTMKAC 2 cut(s) 359, 456
XspI CTAG 4 cut(s) 806, 971, 1041, 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.