FvH4_6g47100

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015224
Physical Location & Seq
Reverse (-)
35789995 .. 35793856
3862 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47100.t1

Sequence Viewer

Length: 1581 bp
ATGACGATGAAGCACCTCCTCACTGCGCTGGCTCGCCGCCGGTCGCCGGCGATGAAGCAGGCCGTCCGATCCAGCTCCACGGCCGTCGCAGGCCCCTCGCTCTCAACCCCGCTCCCCAGGCCGCCGCCGCCGACCGCCATGGTCTACGATCGCCTCGCCGAGGACGTCAAATCGAAGCTCCAGAAGCTCGAGAACCCTGACCCGAGGTTCCTCAAGTACGGGTCGCCTCACCCGGCCGTGACGGATCACTCGCACATCCTCACCGCGCCGGAGACACGTGTCACGGTCCTCCCGAACGGTCTGAGAGTCGCGACGGAGTCGAACCTGGCCGCGAAGACCGCGACGGTCGGGGTGTGGATCGACTCCGGGTCGAGATTCGAGAACGACGAGAACAACGGCACGGCGCATTTTCTGGAGCACATGATCTTCAAAGGGACGAAGAAGAGGACGGCGCTGGATCTGGAGGTGGAGGTGGAGAACATGGGAGGGCATTTGAATGCGTATACGTCCAGGGAGCAGACGACGTACTACGCTAAGGTGCTGGACAAGGACGTGCCCCAGGCATTGTCGATTTTGGCTGATATCTTGCAGAACTCCAATTTTGATGGGAACCGGATTCGGAGAGAGCGCGATGTGATTTTGAGGGAAATGGAGGAGGTTGAGAAGCAACCTGATGAAGTCGTTTTTGACCATCTACATGCAAGTGCATTCCAGTACTCTCCTTTGGCCAGAACTATTCTTGGGCCTGCTGCAAACATTAAGACTATTTCCAGAGAGCATCTTCAGAATTATATAAAAACACACTACACTGCTCCCAGAATGGTGATTGCTGGTTCAGGAGCTATTAAGCATGAAGATCTTGTTGGGCAAGTTGAGGGTTTGTTTACAAACTTGTCAGCTGATCCAACAACAGCTAGTCAATTAGTTGCAAAAGAACCTTCATTTTTCACCGGTTCTGAGGTCAGAATTGTTGATGATGACTTGCCTTATGCAAATTTTGCTGTTGCATTTGAGGGAGCATCCTGGACAGACCCAGACTCTATTGCTCTAATGGTCATGCAGGCAATGTTGGGCTCATGGAGTAAAGCTATCAATGGTGGAAAGCACATGGGTTCTGAGTTGACACAGAGAGTTGCTATTAATGGCATAGCAGAAAGCATGATGGCTTTCAACACCAACTACAAGGACACCGGCTTATTCGGCGTTTTCGCTACTGCAGAGCCGGATGGTTTGGATGATTTGGCCTATGCAATAATGTATGAGATAACCAAGTTAGTTTATCGAGTTTCTGATGCTGATGTTGTTCGTGCTTGCAATCAGTTAAAATCTTCTCTCCTCCTACACTTGGATGGAACAAGTCCTGTAGCTGAAGATATTGGGCGCCAGCTACTCACATATGGTCGAAGAATCCCGTTAGCTGAGTTGTTTGCTAGAATTGATGCTGTTGATGCAAGCACTATCAAGCGGGTTGCAAACCGGTTTATTTATGACAGGGATGTTGCAATTGTTGCCATGGGTCCTATTCAGAAATTGCCCGACTACAACTGGTTCAGACGCAGAACATACTGGAACAGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

527

Amino Acids

58.44

Weight (kDa)

6.41

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 102 - 249 8.7e-56 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 255 - 441 9.7e-38 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AccB1I GGYRCC 1 cut(s) 1380
AccBSI CCGCTC 1 cut(s) 112
AccI GTMKAC 2 cut(s) 144, 503
AccII CGCG 5 cut(s) 266, 311, 332, 341, 630
AclWI GGATC 5 cut(s) 63, 252, 365, 465, 896
AcoI YGGCCR 4 cut(s) 81, 234, 327, 726
AcsI RAATTY 1 cut(s) 994
AcuI CTGAAG 2 cut(s) 767, 1389
AcvI CACGTG 1 cut(s) 278
AcyI GRCGYC 2 cut(s) 165, 1381
AfaI GTAC 3 cut(s) 218, 527, 716
AfiI CCNNNNNNNGG 3 cut(s) 46, 160, 1345
AflIII ACRYGT 2 cut(s) 275, 277
AgeI ACCGGT 2 cut(s) 950, 1476
AgsI TTSAA 3 cut(s) 430, 496, 1171
AhdI GACNNNNNGTC 1 cut(s) 367
AjiI CACGTC 1 cut(s) 553
AjnI CCWGG 5 cut(s) 116, 324, 509, 558, 1022
AjuI GAANNNNNNNTTGG 2 cut(s) 846, 878
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 5 cut(s) 63, 252, 365, 465, 896
Ama87I CYCGRG 2 cut(s) 188, 202
AoxI GGCC 9 cut(s) 60, 81, 91, 119, 234, 327, 726, 743, 1242
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 1 cut(s) 994
AseI ATTAAT 1 cut(s) 1140
AsiGI ACCGGT 2 cut(s) 950, 1476
AspLEI GCGC 6 cut(s) 28, 268, 406, 454, 630, 1383
AspS9I GGNCC 4 cut(s) 92, 286, 743, 1517
AsuC2I CCSGG 2 cut(s) 233, 367
AsuHPI GGTGA 4 cut(s) 221, 253, 835, 940
AvaI CYCGRG 2 cut(s) 188, 202
AvaII GGWCC 2 cut(s) 286, 1517
BaeGI GKGCMC 1 cut(s) 558
BalI TGGCCA 1 cut(s) 728
BanI GGYRCC 1 cut(s) 1380
BanII GRGCYC 1 cut(s) 1076
BbrPI CACGTG 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 420
BbvI GCAGC 1 cut(s) 736
BccI CCATC 5 cut(s) 599, 699, 1156, 1220, 1343
BceAI ACGGC 7 cut(s) 47, 68, 96, 221, 412, 417, 465
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 5 cut(s) 118, 326, 511, 560, 1024
BcnI CCSGG 2 cut(s) 233, 367
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 3 cut(s) 915, 1431, 1579
BfmI CTRYAG 2 cut(s) 1215, 1362
BfoI RGCGCY 2 cut(s) 455, 1384
BglII AGATCT 1 cut(s) 856
BisI GCNGC 6 cut(s) 37, 122, 125, 128, 330, 750
BlsI GCNGC 6 cut(s) 38, 123, 126, 129, 331, 751
BmcAI AGTACT 1 cut(s) 716
Bme1390I CCNGG 7 cut(s) 118, 233, 326, 367, 511, 560, 1024
Bme18I GGWCC 2 cut(s) 286, 1517
BmeRI GACNNNNNGTC 1 cut(s) 367
BmeT110I CYCGRG 2 cut(s) 188, 202
BmgBI CACGTC 1 cut(s) 553
BmgT120I GGNCC 4 cut(s) 92, 286, 743, 1517
BmiI GGNNCC 5 cut(s) 94, 209, 611, 1382, 1518
BmrFI CCNGG 7 cut(s) 118, 233, 326, 367, 511, 560, 1024
BmsI GCATC 5 cut(s) 787, 1028, 1282, 1429, 1438
BoxI GACNNNNGTC 1 cut(s) 278
BpiI GAAGAC 1 cut(s) 341
BpmI CTGGAG 3 cut(s) 164, 434, 482
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 197
BpuMI CCSGG 2 cut(s) 233, 367
BsaAI YACGTR 1 cut(s) 278
BsaHI GRCGYC 2 cut(s) 165, 1381
BsaJI CCNNGG 8 cut(s) 78, 116, 138, 159, 203, 510, 558, 1512
BsaWI WCCGGW 3 cut(s) 612, 950, 1476
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 160, 1345
Bse118I RCCGGY 5 cut(s) 39, 46, 950, 1190, 1476
Bse1I ACTGG 3 cut(s) 712, 1550, 1571
Bse3DI GCAATG 1 cut(s) 1071
BseBI CCWGG 5 cut(s) 118, 326, 511, 560, 1024
BseDI CCNNGG 8 cut(s) 78, 116, 138, 159, 203, 510, 558, 1512
BseGI GGATG 6 cut(s) 255, 1019, 1231, 1240, 1354, 1501
BseLI CCNNNNNNNGG 3 cut(s) 46, 160, 1345
BseMI GCAATG 1 cut(s) 1071
BseMII CTCAG 4 cut(s) 293, 948, 1107, 1410
BseNI ACTGG 3 cut(s) 712, 1550, 1571
BseRI GAGGAG 3 cut(s) 8, 668, 1325
BseSI GKGCMC 1 cut(s) 558
BseX3I CGGCCG 2 cut(s) 81, 234
BseXI GCAGC 1 cut(s) 736
Bsh1236I CGCG 5 cut(s) 266, 311, 332, 341, 630
Bsh1285I CGRYCG 6 cut(s) 44, 84, 135, 151, 237, 348
BshFI GGCC 9 cut(s) 62, 83, 93, 121, 236, 329, 728, 745, 1244
BshNI GGYRCC 1 cut(s) 1380
BshTI ACCGGT 2 cut(s) 950, 1476
BsiEI CGRYCG 6 cut(s) 44, 84, 135, 151, 237, 348
BsiHKAI GWGCWC 1 cut(s) 420
BsiHKCI CYCGRG 2 cut(s) 188, 202
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 3 cut(s) 46, 160, 1345
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 2 cut(s) 502, 707
BsnI GGCC 9 cut(s) 62, 83, 93, 121, 236, 329, 728, 745, 1244
BsoBI CYCGRG 2 cut(s) 188, 202
Bsp1286I GDGCHC 3 cut(s) 420, 558, 1076
Bsp143I GATC 8 cut(s) 68, 148, 244, 357, 423, 457, 856, 901
Bsp19I CCATGG 2 cut(s) 138, 1512
Bsp68I TCGCGA 1 cut(s) 311
BspANI GGCC 9 cut(s) 62, 83, 93, 121, 236, 329, 728, 745, 1244
BspCNI CTCAG 4 cut(s) 294, 949, 1108, 1411
BspFNI CGCG 5 cut(s) 266, 311, 332, 341, 630
BspLI GGNNCC 5 cut(s) 94, 209, 611, 1382, 1518
BspMAI CTGCAG 1 cut(s) 1219
BspPI GGATC 5 cut(s) 63, 252, 365, 465, 896
BspT107I GGYRCC 1 cut(s) 1380
BsrBI CCGCTC 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 1071
BsrFI RCCGGY 5 cut(s) 39, 46, 950, 1190, 1476
BsrI ACTGG 3 cut(s) 712, 1550, 1571
BssAI RCCGGY 5 cut(s) 39, 46, 950, 1190, 1476
BssECI CCNNGG 8 cut(s) 78, 116, 138, 159, 203, 510, 558, 1512
BssMI GATC 8 cut(s) 68, 148, 244, 357, 423, 457, 856, 901
BssNAI GTATAC 1 cut(s) 504
BssNI GRCGYC 2 cut(s) 165, 1381
BssT1I CCWWGG 2 cut(s) 138, 1512
Bst1107I GTATAC 1 cut(s) 504
Bst2UI CCWGG 5 cut(s) 118, 326, 511, 560, 1024
Bst4CI ACNGT 3 cut(s) 286, 299, 346
Bst6I CTCTTC 1 cut(s) 437
BstACI GRCGYC 2 cut(s) 165, 1381
BstAPI GCANNNNNTGC 2 cut(s) 998, 1508
BstBAI YACGTR 1 cut(s) 278
BstDEI CTNAG 5 cut(s) 302, 534, 957, 1116, 1419
BstDSI CCRYGG 3 cut(s) 78, 138, 1512
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 6 cut(s) 255, 1019, 1231, 1240, 1354, 1501
BstFNI CGCG 5 cut(s) 266, 311, 332, 341, 630
BstH2I RGCGCY 2 cut(s) 455, 1384
BstHHI GCGC 6 cut(s) 28, 268, 406, 454, 630, 1383
BstKTI GATC 8 cut(s) 71, 151, 247, 360, 426, 460, 859, 904
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 8 cut(s) 68, 148, 244, 357, 423, 457, 856, 901
BstMCI CGRYCG 6 cut(s) 44, 84, 135, 151, 237, 348
BstMWI GCNNNNNNNGC 8 cut(s) 118, 127, 184, 338, 998, 1200, 1448, 1508
BstNI CCWGG 5 cut(s) 118, 326, 511, 560, 1024
BstNSI RCATGY 1 cut(s) 701
BstPAI GACNNNNGTC 1 cut(s) 278
BstSCI CCNGG 7 cut(s) 116, 231, 324, 365, 509, 558, 1022
BstSFI CTRYAG 2 cut(s) 1215, 1362
BstSLI GKGCMC 1 cut(s) 558
BstUI CGCG 5 cut(s) 266, 311, 332, 341, 630
BstV1I GCAGC 1 cut(s) 736
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 2 cut(s) 457, 856
BstYI RGATCY 2 cut(s) 457, 856
BstZ17I GTATAC 1 cut(s) 504
BstZI CGGCCG 2 cut(s) 81, 234
BsuRI GGCC 9 cut(s) 62, 83, 93, 121, 236, 329, 728, 745, 1244
BtgI CCRYGG 3 cut(s) 78, 138, 1512
BtgZI GCGATG 2 cut(s) 65, 645
BtrI CACGTC 1 cut(s) 553
BtsCI GGATG 6 cut(s) 255, 1019, 1231, 1240, 1354, 1501
BtsI GCAGTG 2 cut(s) 21, 807
BtsIMutI CAGTG 2 cut(s) 21, 807
BtuMI TCGCGA 1 cut(s) 311
CfoI GCGC 6 cut(s) 28, 268, 406, 454, 630, 1383
Cfr10I RCCGGY 5 cut(s) 39, 46, 950, 1190, 1476
Cfr13I GGNCC 4 cut(s) 92, 286, 743, 1517
CseI GACGC 1 cut(s) 1563
Csp6I GTAC 3 cut(s) 217, 526, 715
CspAI ACCGGT 2 cut(s) 950, 1476
CviQI GTAC 3 cut(s) 217, 526, 715
DdeI CTNAG 5 cut(s) 302, 534, 957, 1116, 1419
DinI GGCGCC 1 cut(s) 1382
DpnI GATC 8 cut(s) 70, 150, 246, 359, 425, 459, 858, 903
DpnII GATC 8 cut(s) 68, 148, 244, 357, 423, 457, 856, 901
DriI GACNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 4 cut(s) 81, 234, 327, 726
EagI CGGCCG 2 cut(s) 81, 234
Eam1104I CTCTTC 1 cut(s) 437
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 437
EclXI CGGCCG 2 cut(s) 81, 234
Eco130I CCWWGG 2 cut(s) 138, 1512
Eco24I GRGCYC 1 cut(s) 1076
Eco32I GATATC 1 cut(s) 583
Eco47I GGWCC 2 cut(s) 286, 1517
Eco52I CGGCCG 2 cut(s) 81, 234
Eco57I CTGAAG 2 cut(s) 767, 1389
Eco72I CACGTG 1 cut(s) 278
Eco88I CYCGRG 2 cut(s) 188, 202
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 2 cut(s) 92, 1517
EcoRII CCWGG 5 cut(s) 116, 324, 509, 558, 1022
EcoRV GATATC 1 cut(s) 583
EcoT14I CCWWGG 2 cut(s) 138, 1512
EcoT38I GRGCYC 1 cut(s) 1076
EgeI GGCGCC 1 cut(s) 1382
EheI GGCGCC 1 cut(s) 1382
ErhI CCWWGG 2 cut(s) 138, 1512
FaqI GGGAC 1 cut(s) 448
FauI CCCGC 2 cut(s) 117, 1458
FauNDI CATATG 1 cut(s) 1396
FblI GTMKAC 2 cut(s) 144, 503
Fnu4HI GCNGC 6 cut(s) 37, 122, 125, 128, 330, 750
FokI GGATG 6 cut(s) 242, 1006, 1238, 1247, 1361, 1508
FriOI GRGCYC 1 cut(s) 1076
Fsp4HI GCNGC 6 cut(s) 37, 122, 125, 128, 330, 750
FspBI CTAG 3 cut(s) 915, 1431, 1579
GlaI GCGC 6 cut(s) 27, 267, 405, 453, 629, 1382
GluI GCNGC 6 cut(s) 37, 122, 125, 128, 330, 750
GsuI CTGGAG 3 cut(s) 164, 434, 482
HaeII RGCGCY 2 cut(s) 455, 1384
HaeIII GGCC 9 cut(s) 62, 83, 93, 121, 236, 329, 728, 745, 1244
HgaI GACGC 1 cut(s) 1563
HhaI GCGC 6 cut(s) 28, 268, 406, 454, 630, 1383
Hin1I GRCGYC 2 cut(s) 165, 1381
Hin6I GCGC 6 cut(s) 26, 266, 404, 452, 628, 1381
HinP1I GCGC 6 cut(s) 26, 266, 404, 452, 628, 1381
HincII GTYRAC 1 cut(s) 1122
HindII GTYRAC 1 cut(s) 1122
HinfI GANTC 7 cut(s) 306, 317, 362, 375, 616, 1037, 1407
HphI GGTGA 4 cut(s) 221, 253, 835, 940
Hpy166II GTNNAC 4 cut(s) 145, 504, 885, 1122
Hpy8I GTNNAC 4 cut(s) 145, 504, 885, 1122
Hpy99I CGWCG 5 cut(s) 89, 316, 346, 389, 526
HpyAV CCTTC 1 cut(s) 948
HpyCH4III ACNGT 3 cut(s) 286, 299, 346
HpyCH4IV ACGT 5 cut(s) 165, 277, 506, 524, 552
HpyF10VI GCNNNNNNNGC 8 cut(s) 118, 127, 184, 338, 998, 1200, 1448, 1508
HpyF3I CTNAG 5 cut(s) 302, 534, 957, 1116, 1419
HpySE526I ACGT 5 cut(s) 165, 277, 506, 524, 552
Hsp92I GRCGYC 2 cut(s) 165, 1381
HspAI GCGC 6 cut(s) 26, 266, 404, 452, 628, 1381
KasI GGCGCC 1 cut(s) 1380
KroI GCCGGC 1 cut(s) 46
KroNI GCCGGC 1 cut(s) 48
Kzo9I GATC 8 cut(s) 68, 148, 244, 357, 423, 457, 856, 901
LmnI GCTCC 8 cut(s) 80, 117, 183, 415, 514, 817, 839, 1016
Lsp1109I GCAGC 1 cut(s) 736
LweI GCATC 5 cut(s) 787, 1028, 1282, 1429, 1438
MaeI CTAG 3 cut(s) 915, 1431, 1579
MaeII ACGT 5 cut(s) 165, 277, 506, 524, 552
MaeIII GTNAC 2 cut(s) 238, 280
MalI GATC 8 cut(s) 70, 150, 246, 359, 425, 459, 858, 903
MbiI CCGCTC 1 cut(s) 112
MboI GATC 8 cut(s) 68, 148, 244, 357, 423, 457, 856, 901
MboII GAAGA 9 cut(s) 346, 418, 451, 454, 773, 866, 1320, 1382, 1416
MfeI CAATTG 1 cut(s) 1503
MflI RGATCY 2 cut(s) 457, 856
MhlI GDGCHC 3 cut(s) 420, 558, 1076
MlsI TGGCCA 1 cut(s) 728
MluCI AATT 8 cut(s) 598, 787, 920, 966, 994, 1434, 1503, 1529
MluNI TGGCCA 1 cut(s) 728
Mly113I GGCGCC 1 cut(s) 1381
MlyI GAGTC 4 cut(s) 315, 326, 356, 1031
MmeI TCCRAC 1 cut(s) 929
Mox20I TGGCCA 1 cut(s) 728
MreI CGCCGGCG 1 cut(s) 46
MroNI GCCGGC 1 cut(s) 46
MscI TGGCCA 1 cut(s) 728
MseI TTAA 4 cut(s) 759, 846, 1140, 1322
MslI CAYNNNNRTG 4 cut(s) 495, 696, 702, 1347
Msp20I TGGCCA 1 cut(s) 728
MspA1I CMGCKG 1 cut(s) 899
MspR9I CCNGG 7 cut(s) 118, 233, 326, 367, 511, 560, 1024
MunI CAATTG 1 cut(s) 1503
Mva1269I GAATGC 2 cut(s) 502, 707
MvaI CCWGG 5 cut(s) 118, 326, 511, 560, 1024
MvnI CGCG 5 cut(s) 266, 311, 332, 341, 630
MwoI GCNNNNNNNGC 8 cut(s) 118, 127, 184, 338, 998, 1200, 1448, 1508
NaeI GCCGGC 1 cut(s) 48
NarI GGCGCC 1 cut(s) 1381
NciI CCSGG 2 cut(s) 233, 367
NcoI CCATGG 2 cut(s) 138, 1512
NdeI CATATG 1 cut(s) 1396
NdeII GATC 8 cut(s) 68, 148, 244, 357, 423, 457, 856, 901
NgoMIV GCCGGC 1 cut(s) 46
NlaIV GGNNCC 5 cut(s) 94, 209, 611, 1382, 1518
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 2 cut(s) 238, 280
NruI TCGCGA 1 cut(s) 311
NspI RCATGY 1 cut(s) 701
PaeR7I CTCGAG 1 cut(s) 188
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 290, 384
PctI GAATGC 2 cut(s) 502, 707
PdiI GCCGGC 1 cut(s) 48
PfeI GAWTC 3 cut(s) 375, 616, 1407
PflFI GACNNNGTC 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 1022
PinAI ACCGGT 2 cut(s) 950, 1476
PkrI GCNGC 6 cut(s) 38, 123, 126, 129, 331, 751
Ple19I CGATCG 1 cut(s) 151
PleI GAGTC 4 cut(s) 314, 325, 356, 1031
PluTI GGCGCC 1 cut(s) 1384
PmaCI CACGTG 1 cut(s) 278
PmlI CACGTG 1 cut(s) 278
PpsI GAGTC 4 cut(s) 314, 325, 356, 1031
Ppu21I YACGTR 1 cut(s) 278
PpuMI RGGWCCY 1 cut(s) 1517
PshAI GACNNNNGTC 1 cut(s) 278
PshBI ATTAAT 1 cut(s) 1140
Psp5II RGGWCCY 1 cut(s) 1517
Psp6I CCWGG 5 cut(s) 116, 324, 509, 558, 1022
PspCI CACGTG 1 cut(s) 278
PspGI CCWGG 5 cut(s) 116, 324, 509, 558, 1022
PspN4I GGNNCC 5 cut(s) 94, 209, 611, 1382, 1518
PspPI GGNCC 4 cut(s) 92, 286, 743, 1517
PspPPI RGGWCCY 1 cut(s) 1517
PstI CTGCAG 1 cut(s) 1219
PsuI RGATCY 2 cut(s) 457, 856
PsyI GACNNNGTC 1 cut(s) 316
PvuI CGATCG 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 899
RruI TCGCGA 1 cut(s) 311
RsaI GTAC 3 cut(s) 218, 527, 716
RsaNI GTAC 3 cut(s) 217, 526, 715
RseI CAYNNNNRTG 4 cut(s) 495, 696, 702, 1347
SaqAI TTAA 4 cut(s) 759, 846, 1140, 1322
SatI GCNGC 6 cut(s) 37, 122, 125, 128, 330, 750
Sau3AI GATC 8 cut(s) 68, 148, 244, 357, 423, 457, 856, 901
Sau96I GGNCC 4 cut(s) 92, 286, 743, 1517
ScaI AGTACT 1 cut(s) 716
SchI GAGTC 4 cut(s) 315, 326, 356, 1031
ScrFI CCNGG 7 cut(s) 118, 233, 326, 367, 511, 560, 1024
SduI GDGCHC 3 cut(s) 420, 558, 1076
SfaNI GCATC 5 cut(s) 787, 1028, 1282, 1429, 1438
SfcI CTRYAG 2 cut(s) 1215, 1362
SfoI GGCGCC 1 cut(s) 1382
Sfr274I CTCGAG 1 cut(s) 188
SgrAI CRCCGGYG 1 cut(s) 46
SinI GGWCC 2 cut(s) 286, 1517
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 4 cut(s) 495, 696, 702, 1347
SmlI CTYRAG 2 cut(s) 188, 212
SmoI CTYRAG 2 cut(s) 188, 212
Sse9I AATT 8 cut(s) 598, 787, 920, 966, 994, 1434, 1503, 1529
SspDI GGCGCC 1 cut(s) 1380
SspMI CTAG 3 cut(s) 915, 1431, 1579
StyD4I CCNGG 7 cut(s) 116, 231, 324, 365, 509, 558, 1022
StyI CCWWGG 2 cut(s) 138, 1512
TaaI ACNGT 3 cut(s) 286, 299, 346
TaiI ACGT 5 cut(s) 168, 280, 509, 527, 555
TaqI TCGA 9 cut(s) 173, 189, 320, 360, 371, 378, 569, 1282, 1402
TasI AATT 8 cut(s) 598, 787, 920, 966, 994, 1434, 1503, 1529
TatI WGTACW 1 cut(s) 714
TauI GCSGC 5 cut(s) 39, 124, 127, 130, 332
TfiI GAWTC 3 cut(s) 375, 616, 1407
Tru1I TTAA 4 cut(s) 759, 846, 1140, 1322
Tru9I TTAA 4 cut(s) 759, 846, 1140, 1322
TscAI CASTG 2 cut(s) 28, 814
TseFI GTSAC 2 cut(s) 238, 280
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 2 cut(s) 238, 280
TspDTI ATGAA 5 cut(s) 23, 68, 690, 867, 930
TspGWI ACGGA 2 cut(s) 257, 329
TspRI CASTG 2 cut(s) 28, 814
Tth111I GACNNNGTC 1 cut(s) 316
VpaK11BI GGWCC 2 cut(s) 286, 1517
VspI ATTAAT 1 cut(s) 1140
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 1 cut(s) 994
XceI RCATGY 1 cut(s) 701
XhoI CTCGAG 1 cut(s) 188
XmiI GTMKAC 2 cut(s) 144, 503
XspI CTAG 3 cut(s) 915, 1431, 1579
ZraI GACGTC 1 cut(s) 166
ZrmI AGTACT 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.