pycom111g05370

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0015224
Physical Location & Seq
Forward (+)
3448504 .. 3449160
657 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g05370.1

Sequence Viewer

Length: 594 bp
ATGGCAGCTGAGCCCCTGGAACACTGTTCGCAGTTTCCCCCAAACCGAAACCCTAGAAGACCTCTGACTGAGACAGCCATGGCGATGAAGCACCTTCTTACAACCCTAGCTCGCCGACGGCCCCACAGACCGCCGGCTGCCCTAACAGCAGCCGTTCGATCCCAGTCAACCTCACCCGCCGTCGCCACGTCATCTTCATCCCCATCCCTACCCTCCCCTCCTCCACCAACCGCCATGGTCTACGATCGATTGGCCGAGGAAGTCAAATCGAAGATCAAAAGGCTCGAGAATCCGGACCCGAGGTTCCTCAAGTACGGGTCGCCCCACCCGAGTCTCACGGACCACAGCCACATCCTCACCGCACCCGAGACACGTGTCACCACGCTCCCCAACGGCCTCCGGGTTGCCACCGAGTCAAACCTCGCGTCTAAGACCGCCACCGTCGGGGTCTGGATCGACGCCGGGTCAAGGTTCGAGAACGACGAGACCAACGGCACCGCACATTTCTTGGAGCACATGATCGATTCAAGGGGACGGAGCAGAGGACCGCGCGTGATCTCGAGGAGGAGGTCGAGAACATGGGAGGCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

198

Amino Acids

21.75

Weight (kDa)

10.76

Isoelectric Point (pI)

73.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 134 - 174 6.7e-14 Insulinase (Peptidase family M16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 494
AccI GTMKAC 1 cut(s) 240
AccII CGCG 3 cut(s) 425, 550, 552
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 7 cut(s) 131, 177, 231, 360, 435, 498, 548
AclWI GGATC 2 cut(s) 153, 461
AcoI YGGCCR 1 cut(s) 252
AcvI CACGTG 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 2 cut(s) 444, 468
AflIII ACRYGT 2 cut(s) 371, 373
AgsI TTSAA 1 cut(s) 528
AhdI GACNNNNNGTC 1 cut(s) 463
AjiI CACGTC 1 cut(s) 189
AjnI CCWGG 1 cut(s) 15
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
AluBI AGCT 2 cut(s) 8, 110
AluI AGCT 2 cut(s) 8, 110
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 4 cut(s) 65, 338, 362, 479
AlwI GGATC 2 cut(s) 153, 461
Ama87I CYCGRG 5 cut(s) 284, 298, 328, 365, 559
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 4 cut(s) 119, 252, 394, 585
ApeKI GCWGC 3 cut(s) 5, 137, 149
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AspLEI GCGC 1 cut(s) 552
AspS9I GGNCC 4 cut(s) 120, 295, 340, 545
AsuC2I CCSGG 2 cut(s) 401, 463
AsuHPI GGTGA 3 cut(s) 165, 349, 370
AvaI CYCGRG 5 cut(s) 284, 298, 328, 365, 559
AvaII GGWCC 3 cut(s) 295, 340, 545
BanI GGYRCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 15
BbrPI CACGTG 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 516
BbvI GCAGC 3 cut(s) 17, 124, 161
BccI CCATC 1 cut(s) 211
BceAI ACGGC 5 cut(s) 134, 137, 164, 409, 508
BciT130I CCWGG 1 cut(s) 17
BcnI CCSGG 2 cut(s) 401, 463
BcoDI GTCTC 4 cut(s) 65, 338, 362, 479
BfaI CTAG 2 cut(s) 54, 107
BisI GCNGC 3 cut(s) 6, 138, 150
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 3 cut(s) 7, 139, 151
Bme1390I CCNGG 3 cut(s) 17, 401, 463
Bme18I GGWCC 3 cut(s) 295, 340, 545
BmeRI GACNNNNNGTC 1 cut(s) 463
BmeT110I CYCGRG 5 cut(s) 284, 298, 328, 365, 559
BmgBI CACGTC 1 cut(s) 189
BmgT120I GGNCC 4 cut(s) 120, 295, 340, 545
BmiI GGNNCC 4 cut(s) 122, 297, 305, 496
BmrFI CCNGG 3 cut(s) 17, 401, 463
BmrI ACTGGG 1 cut(s) 157
BmuI ACTGGG 1 cut(s) 157
BoxI GACNNNNGTC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 64
Bpu1102I GCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 2 cut(s) 401, 463
Bsa29I ATCGAT 2 cut(s) 247, 522
BsaAI YACGTR 1 cut(s) 374
BsaHI GRCGYC 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 5 cut(s) 15, 78, 234, 255, 299
BsaWI WCCGGW 1 cut(s) 292
Bsc4I CCNNNNNNNGG 2 cut(s) 444, 468
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 163
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 1 cut(s) 17
BseCI ATCGAT 2 cut(s) 247, 522
BseDI CCNNGG 5 cut(s) 15, 78, 234, 255, 299
BseGI GGATG 3 cut(s) 197, 203, 351
BseLI CCNNNNNNNGG 2 cut(s) 444, 468
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 163
BseRI GAGGAG 3 cut(s) 210, 577, 580
BseXI GCAGC 3 cut(s) 17, 124, 161
Bsh1236I CGCG 3 cut(s) 425, 550, 552
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 4 cut(s) 121, 254, 396, 587
BshNI GGYRCC 1 cut(s) 494
BshVI ATCGAT 2 cut(s) 247, 522
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 516
BsiHKCI CYCGRG 5 cut(s) 284, 298, 328, 365, 559
BsiSI CCGG 4 cut(s) 134, 293, 400, 462
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 2 cut(s) 444, 468
BsmAI GTCTC 4 cut(s) 65, 338, 362, 479
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 4 cut(s) 121, 254, 396, 587
Bso31I GGTCTC 1 cut(s) 479
BsoBI CYCGRG 5 cut(s) 284, 298, 328, 365, 559
Bsp1286I GDGCHC 2 cut(s) 15, 516
Bsp13I TCCGGA 1 cut(s) 292
Bsp143I GATC 6 cut(s) 158, 244, 273, 453, 519, 555
Bsp1720I GCTNAGC 1 cut(s) 9
Bsp19I CCATGG 2 cut(s) 78, 234
BspACI CCGC 7 cut(s) 131, 177, 231, 360, 435, 498, 548
BspANI GGCC 4 cut(s) 121, 254, 396, 587
BspCNI CTCAG 1 cut(s) 61
BspDI ATCGAT 2 cut(s) 247, 522
BspEI TCCGGA 1 cut(s) 292
BspFNI CGCG 3 cut(s) 425, 550, 552
BspLI GGNNCC 4 cut(s) 122, 297, 305, 496
BspPI GGATC 2 cut(s) 153, 461
BspT107I GGYRCC 1 cut(s) 494
BspTNI GGTCTC 1 cut(s) 479
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 1 cut(s) 163
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 5 cut(s) 15, 78, 234, 255, 299
BssMI GATC 6 cut(s) 158, 244, 273, 453, 519, 555
BssNI GRCGYC 1 cut(s) 459
BssT1I CCWWGG 2 cut(s) 78, 234
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 2 cut(s) 26, 442
BstACI GRCGYC 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 112, 135
BstDEI CTNAG 3 cut(s) 9, 69, 429
BstDSI CCRYGG 2 cut(s) 78, 234
BstF5I GGATG 3 cut(s) 197, 203, 351
BstFNI CGCG 3 cut(s) 425, 550, 552
BstHHI GCGC 1 cut(s) 552
BstKTI GATC 6 cut(s) 161, 247, 276, 456, 522, 558
BstMAI GTCTC 4 cut(s) 65, 338, 362, 479
BstMBI GATC 6 cut(s) 158, 244, 273, 453, 519, 555
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 1 cut(s) 17
BstPAI GACNNNNGTC 1 cut(s) 374
BstSCI CCNGG 3 cut(s) 15, 399, 461
BstUI CGCG 3 cut(s) 425, 550, 552
BstV1I GCAGC 3 cut(s) 17, 124, 161
BstV2I GAAGAC 1 cut(s) 64
Bsu15I ATCGAT 2 cut(s) 247, 522
BsuRI GGCC 4 cut(s) 121, 254, 396, 587
BsuTUI ATCGAT 2 cut(s) 247, 522
BtgI CCRYGG 2 cut(s) 78, 234
BtgZI GCGATG 1 cut(s) 98
BtrI CACGTC 1 cut(s) 189
BtsCI GGATG 3 cut(s) 197, 203, 351
BtsIMutI CAGTG 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 112, 135
CfoI GCGC 1 cut(s) 552
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 4 cut(s) 120, 295, 340, 545
ClaI ATCGAT 2 cut(s) 247, 522
CseI GACGC 2 cut(s) 414, 467
Csp6I GTAC 1 cut(s) 313
CviAII CATG 4 cut(s) 79, 235, 517, 579
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 3 cut(s) 9, 69, 429
DpnI GATC 6 cut(s) 160, 246, 275, 455, 521, 557
DpnII GATC 6 cut(s) 158, 244, 273, 453, 519, 555
DriI GACNNNNNGTC 1 cut(s) 463
EaeI YGGCCR 1 cut(s) 252
Eam1105I GACNNNNNGTC 1 cut(s) 463
Eco130I CCWWGG 2 cut(s) 78, 234
Eco24I GRGCYC 1 cut(s) 15
Eco31I GGTCTC 1 cut(s) 479
Eco47I GGWCC 3 cut(s) 295, 340, 545
Eco72I CACGTG 1 cut(s) 374
Eco88I CYCGRG 5 cut(s) 284, 298, 328, 365, 559
EcoRII CCWGG 1 cut(s) 15
EcoT14I CCWWGG 2 cut(s) 78, 234
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 2 cut(s) 78, 234
FaeI CATG 4 cut(s) 82, 238, 520, 582
FaiI YATR 4 cut(s) 80, 236, 518, 580
FaqI GGGAC 1 cut(s) 546
FatI CATG 4 cut(s) 78, 234, 516, 578
FauI CCCGC 1 cut(s) 184
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 3 cut(s) 6, 138, 150
FokI GGATG 3 cut(s) 184, 190, 338
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 3 cut(s) 6, 138, 150
FspBI CTAG 2 cut(s) 54, 107
GlaI GCGC 1 cut(s) 551
GluI GCNGC 3 cut(s) 6, 138, 150
HaeIII GGCC 4 cut(s) 121, 254, 396, 587
HapII CCGG 4 cut(s) 134, 293, 400, 462
HgaI GACGC 2 cut(s) 414, 467
HhaI GCGC 1 cut(s) 552
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 4 cut(s) 82, 238, 520, 582
Hin6I GCGC 1 cut(s) 550
HinP1I GCGC 1 cut(s) 550
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HinfI GANTC 4 cut(s) 289, 331, 413, 524
HpaII CCGG 4 cut(s) 134, 293, 400, 462
HphI GGTGA 3 cut(s) 165, 349, 370
Hpy166II GTNNAC 2 cut(s) 168, 241
Hpy188I TCNGA 1 cut(s) 66
Hpy188III TCNNGA 6 cut(s) 286, 293, 451, 475, 559, 573
Hpy8I GTNNAC 2 cut(s) 168, 241
Hpy99I CGWCG 5 cut(s) 120, 185, 446, 461, 485
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 2 cut(s) 26, 442
HpyCH4IV ACGT 2 cut(s) 188, 373
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 3 cut(s) 9, 69, 429
HpySE526I ACGT 2 cut(s) 188, 373
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 4 cut(s) 82, 238, 520, 582
HspAI GCGC 1 cut(s) 550
Kpn2I TCCGGA 1 cut(s) 292
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 6 cut(s) 158, 244, 273, 453, 519, 555
LmnI GCTCC 3 cut(s) 390, 511, 537
LpnPI CCDG 8 cut(s) 2, 29, 147, 176, 306, 413, 436, 475
Lsp1109I GCAGC 3 cut(s) 17, 124, 161
MaeI CTAG 2 cut(s) 54, 107
MaeII ACGT 2 cut(s) 188, 373
MaeIII GTNAC 1 cut(s) 376
MalI GATC 6 cut(s) 160, 246, 275, 455, 521, 557
MboI GATC 6 cut(s) 158, 244, 273, 453, 519, 555
MboII GAAGA 3 cut(s) 69, 186, 283
MhlI GDGCHC 2 cut(s) 15, 516
MlyI GAGTC 2 cut(s) 340, 422
MroI TCCGGA 1 cut(s) 292
MroNI GCCGGC 1 cut(s) 133
MslI CAYNNNNRTG 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 4 cut(s) 134, 293, 400, 462
MspR9I CCNGG 3 cut(s) 17, 401, 463
MvaI CCWGG 1 cut(s) 17
MvnI CGCG 3 cut(s) 425, 550, 552
MwoI GCNNNNNNNGC 1 cut(s) 146
NaeI GCCGGC 1 cut(s) 135
NciI CCSGG 2 cut(s) 401, 463
NcoI CCATGG 2 cut(s) 78, 234
NdeII GATC 6 cut(s) 158, 244, 273, 453, 519, 555
NgoMIV GCCGGC 1 cut(s) 133
NlaIII CATG 4 cut(s) 82, 238, 520, 582
NlaIV GGNNCC 4 cut(s) 122, 297, 305, 496
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 376
PaeR7I CTCGAG 2 cut(s) 284, 559
PcsI WCGNNNNNNNCGW 2 cut(s) 326, 480
PdiI GCCGGC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 289, 524
PkrI GCNGC 3 cut(s) 7, 139, 151
Ple19I CGATCG 1 cut(s) 247
PleI GAGTC 2 cut(s) 339, 421
PmaCI CACGTG 1 cut(s) 374
PmlI CACGTG 1 cut(s) 374
PpsI GAGTC 2 cut(s) 339, 421
Ppu21I YACGTR 1 cut(s) 374
PshAI GACNNNNGTC 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 15
PspCI CACGTG 1 cut(s) 374
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 4 cut(s) 122, 297, 305, 496
PspPI GGNCC 4 cut(s) 120, 295, 340, 545
PvuI CGATCG 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 83
SatI GCNGC 3 cut(s) 6, 138, 150
Sau3AI GATC 6 cut(s) 158, 244, 273, 453, 519, 555
Sau96I GGNCC 4 cut(s) 120, 295, 340, 545
SchI GAGTC 2 cut(s) 340, 422
ScrFI CCNGG 3 cut(s) 17, 401, 463
SduI GDGCHC 2 cut(s) 15, 516
Sfr274I CTCGAG 2 cut(s) 284, 559
SinI GGWCC 3 cut(s) 295, 340, 545
SlaI CTCGAG 2 cut(s) 284, 559
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 3 cut(s) 284, 308, 559
SmoI CTYRAG 3 cut(s) 284, 308, 559
SsiI CCGC 7 cut(s) 131, 177, 231, 360, 435, 498, 548
SspMI CTAG 2 cut(s) 54, 107
StyD4I CCNGG 3 cut(s) 15, 399, 461
StyI CCWWGG 2 cut(s) 78, 234
TaaI ACNGT 2 cut(s) 26, 442
TaiI ACGT 2 cut(s) 191, 376
TaqI TCGA 9 cut(s) 157, 247, 269, 285, 456, 474, 522, 560, 572
TfiI GAWTC 2 cut(s) 289, 524
TscAI CASTG 1 cut(s) 29
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 3 cut(s) 5, 137, 149
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 2 cut(s) 101, 186
TspGWI ACGGA 2 cut(s) 353, 550
TspRI CASTG 1 cut(s) 29
VpaK11BI GGWCC 3 cut(s) 295, 340, 545
XhoI CTCGAG 2 cut(s) 284, 559
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 2 cut(s) 54, 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.