Prupe.3G256900_v2.0.a1

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0015224
Physical Location & Seq
Reverse (-)
24481828 .. 24485482
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G256900.1

Sequence Viewer

Length: 1599 bp
ATGGCGATGAAGCACCTCCTAACAACCATAGCTCGCCGTCGGCCGCACAGACCCCCGGCCGCTCTCACAGTAGCCGTACGATCCTCATCAACCTCACCCGCCGTCGCTGAGTCACCTTTGGCACCATCCTTACCCTCGCCTCCTCCACCATCCGCTATGATCTACGACCGCTTGGCCGAGGACGTCAAATCGAAGATCAGGAGGCTCGAGAACCCGGACCCGAGGTTCCTCAAGTACGGGTCGCCCCACCCGAGTATCGCGGACCATACCCACATCCTGACCGCACCCGAGACACGTGTCACCACACTCCCCAACGGCCTGCGAGTGGCCACCGAGTCGAACCTCGCGGCCCAGACGGCAACCGTCGGGGTCTGGATCGATGCTGGGTCGCGGTTCGAGAACGACGCGACCAACGGCACCGCGCATTTCTTGGAGCACATGATCTTCAAAGGGACCGACCAGAGGTCAGCGCGTCAGCTCGAGGAGGAAGTCGAGAACATGGGAGGTCACTTGAATGCGTACACATCGAGGGAGCAGACCACCTACTATGCCAAGGTGCTGCAGTCGGATGTGCCCAAGGCGCTCTCTATTTTGGCTGATATCTTGCAGAACTCCAAGTTTGATGATAACCGTATTCTTCGCGAGCGCGAAGTGATTTTGAGGGAAATGGAGGAGGTCGAGAAACAACCCGATGAAGTTATTTTTGACCATCTGCATGCAACTGCATTCCAGTACTCTCCTCTAGCCAGAACTATTCTTGGACCTGCTAACAATATTAAGACTATCTCCAAAGAACATCTTCAAAGCTATATCAAAACTCACTATACCGCACCCAGAATGGTTATTGCTGCTTCAGGAGCTGTTAGGCATGAAGATATTGTTGAGACAGTTAAAGGTTTGTTTACAAAATTGTCAGGAGATCCAACCACGGCTAGTCAGTTGGTTGCAAAAGAACCTTCCTTTTTTACTGGTTCTGAGGTCAGAATGTTTGATAGTGACTTGCCTTTGGCACATTTCGCGGTTGCATTCAATGGAGCATCTTGGACAGATCCAGACTCTATTCCTCTAATGGTCATGCAGGCTATGTTAGGTTCATGGAATAAAAATGCTGGTGGTGGAAAGCACATGGGTTCAGAGCTGGCACAACGGGTTGCCATAAATGACATAGCAGACAGCTATATGGCTTTCAACACCAACTATAAGGATACTGGCTTATTTGGTGTTTTTGCTACTGCTAAGCCGGATTGCTTAGATGATTTGGCCTATTCAATAATGTATGAAATAACCAAATTAGTTTATCGAGTTTCAGAAGCTGACGTGATCCGTGCTCGTAATCAGTTGAAATCTTCCCTGCTCCTTCACTTGGATGGAACAAGTGCCGTAGCTGAAGATATTGGGCGCCAGCTACTTACATATGGTCGAAGAATCCCATTAGCTGAATTGTTTGCTAGAATTGATGCTGTTAATGCAAGCACCATCAAACGTGTTGCAAACCGATTTATCTATGACAGGGATATTGCAATTGCTTCCATGGGTCCTGTCGGGGAGTTGCCCGACTACAACTGGTTCAGACGCCGGACCTACTGGAACAGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

533

Amino Acids

59.22

Weight (kDa)

6.78

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 772
AccB1I GGYRCC 3 cut(s) 121, 416, 1398
AccBSI CCGCTC 1 cut(s) 62
AccII CGCG 9 cut(s) 260, 347, 391, 407, 422, 472, 642, 648, 1019
AclWI GGATC 5 cut(s) 75, 383, 914, 1043, 1315
AcoI YGGCCR 4 cut(s) 41, 57, 174, 327
AcuI CTGAAG 2 cut(s) 837, 1407
AcvI CACGTG 1 cut(s) 296
AcyI GRCGYC 3 cut(s) 183, 1399, 1573
AfaI GTAC 4 cut(s) 78, 236, 521, 734
AfiI CCNNNNNNNGG 3 cut(s) 325, 462, 1363
AflIII ACRYGT 3 cut(s) 293, 295, 1483
AgsI TTSAA 7 cut(s) 448, 514, 803, 1030, 1189, 1269, 1342
AhdI GACNNNNNGTC 1 cut(s) 463
AjiI CACGTC 1 cut(s) 1318
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
Alw21I GWGCWC 2 cut(s) 438, 1330
Alw26I GTCTC 2 cut(s) 284, 878
AlwI GGATC 5 cut(s) 75, 383, 914, 1043, 1315
AlwNI CAGNNNCTG 2 cut(s) 860, 1313
Ama87I CYCGRG 5 cut(s) 206, 220, 250, 287, 479
AoxI GGCC 7 cut(s) 41, 57, 174, 316, 327, 348, 1260
ApeKI GCWGC 2 cut(s) 559, 848
Asp700I GAANNNNTTC 1 cut(s) 798
AspLEI GCGC 5 cut(s) 424, 472, 583, 648, 1401
AspS9I GGNCC 7 cut(s) 217, 262, 349, 453, 761, 1535, 1578
AsuC2I CCSGG 2 cut(s) 56, 215
AsuHPI GGTGA 3 cut(s) 87, 105, 292
AvaI CYCGRG 5 cut(s) 206, 220, 250, 287, 479
AvaII GGWCC 6 cut(s) 217, 262, 453, 761, 1535, 1578
BaeGI GKGCMC 1 cut(s) 576
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 3 cut(s) 121, 416, 1398
BbrPI CACGTG 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 438, 1330
BbvI GCAGC 2 cut(s) 546, 835
BccI CCATC 5 cut(s) 133, 157, 717, 1361, 1484
BceAI ACGGC 8 cut(s) 21, 59, 86, 331, 372, 430, 945, 1364
BciVI GTATCC 1 cut(s) 1198
BcnI CCSGG 2 cut(s) 56, 215
BcoDI GTCTC 2 cut(s) 284, 878
BfaI CTAG 3 cut(s) 743, 933, 1449
BfmI CTRYAG 1 cut(s) 560
BfoI RGCGCY 2 cut(s) 584, 1402
BfuAI ACCTGC 1 cut(s) 772
BfuI GTATCC 1 cut(s) 1198
BglI GCCNNNNNGGC 1 cut(s) 356
BisI GCNGC 5 cut(s) 44, 60, 348, 560, 849
BlpI GCTNAGC 1 cut(s) 1236
BlsI GCNGC 5 cut(s) 45, 61, 349, 561, 850
BmcAI AGTACT 1 cut(s) 734
Bme1390I CCNGG 2 cut(s) 56, 215
Bme18I GGWCC 6 cut(s) 217, 262, 453, 761, 1535, 1578
BmeRI GACNNNNNGTC 1 cut(s) 463
BmeT110I CYCGRG 5 cut(s) 206, 220, 250, 287, 479
BmgBI CACGTC 1 cut(s) 1318
BmgT120I GGNCC 7 cut(s) 217, 262, 349, 453, 761, 1535, 1578
BmiI GGNNCC 7 cut(s) 123, 219, 227, 418, 454, 1400, 1536
BmrFI CCNGG 2 cut(s) 56, 215
BmsI GCATC 3 cut(s) 370, 1046, 1447
BoxI GACNNNNGTC 1 cut(s) 296
Bpu1102I GCTNAGC 1 cut(s) 1236
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 2 cut(s) 56, 215
Bsa29I ATCGAT 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 85
BsaHI GRCGYC 3 cut(s) 183, 1399, 1573
BsaJI CCNNGG 7 cut(s) 54, 177, 221, 552, 576, 927, 1530
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 462, 1363
Bse1I ACTGG 5 cut(s) 730, 973, 1213, 1568, 1589
Bse8I GATNNNNATC 1 cut(s) 85
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 7 cut(s) 54, 177, 221, 552, 576, 927, 1530
BseGI GGATG 5 cut(s) 125, 149, 273, 574, 1372
BseJI GATNNNNATC 1 cut(s) 85
BseLI CCNNNNNNNGG 3 cut(s) 325, 462, 1363
BseMII CTCAG 2 cut(s) 99, 966
BseNI ACTGG 5 cut(s) 730, 973, 1213, 1568, 1589
BseRI GAGGAG 4 cut(s) 132, 497, 686, 729
BseSI GKGCMC 1 cut(s) 576
BseX3I CGGCCG 2 cut(s) 41, 57
BseXI GCAGC 2 cut(s) 546, 835
BseYI CCCAGC 1 cut(s) 383
Bsh1236I CGCG 9 cut(s) 260, 347, 391, 407, 422, 472, 642, 648, 1019
Bsh1285I CGRYCG 3 cut(s) 44, 60, 169
BshFI GGCC 7 cut(s) 43, 59, 176, 318, 329, 350, 1262
BshNI GGYRCC 3 cut(s) 121, 416, 1398
BshVI ATCGAT 1 cut(s) 378
BsiEI CGRYCG 3 cut(s) 44, 60, 169
BsiHKAI GWGCWC 2 cut(s) 438, 1330
BsiHKCI CYCGRG 5 cut(s) 206, 220, 250, 287, 479
BsiSI CCGG 4 cut(s) 56, 215, 1241, 1576
BsiWI CGTACG 1 cut(s) 76
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 3 cut(s) 325, 462, 1363
BsmAI GTCTC 2 cut(s) 284, 878
BsmFI GGGAC 1 cut(s) 466
BsmI GAATGC 3 cut(s) 520, 725, 1025
BsnI GGCC 7 cut(s) 43, 59, 176, 318, 329, 350, 1262
BsoBI CYCGRG 5 cut(s) 206, 220, 250, 287, 479
Bsp1286I GDGCHC 3 cut(s) 438, 576, 1330
Bsp143I GATC 8 cut(s) 80, 159, 195, 375, 441, 919, 1048, 1320
Bsp1720I GCTNAGC 1 cut(s) 1236
Bsp19I CCATGG 1 cut(s) 1530
Bsp68I TCGCGA 1 cut(s) 642
BspANI GGCC 7 cut(s) 43, 59, 176, 318, 329, 350, 1262
BspCNI CTCAG 2 cut(s) 100, 967
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 9 cut(s) 260, 347, 391, 407, 422, 472, 642, 648, 1019
BspLI GGNNCC 7 cut(s) 123, 219, 227, 418, 454, 1400, 1536
BspMAI CTGCAG 1 cut(s) 564
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 5 cut(s) 75, 383, 914, 1043, 1315
BspT107I GGYRCC 3 cut(s) 121, 416, 1398
BsrBI CCGCTC 1 cut(s) 62
BsrI ACTGG 5 cut(s) 730, 973, 1213, 1568, 1589
BssECI CCNNGG 7 cut(s) 54, 177, 221, 552, 576, 927, 1530
BssMI GATC 8 cut(s) 80, 159, 195, 375, 441, 919, 1048, 1320
BssNI GRCGYC 3 cut(s) 183, 1399, 1573
BssT1I CCWWGG 3 cut(s) 552, 576, 1530
Bst4CI ACNGT 4 cut(s) 70, 364, 632, 889
BstACI GRCGYC 3 cut(s) 183, 1399, 1573
BstBAI YACGTR 1 cut(s) 296
BstC8I GCNNGC 8 cut(s) 34, 320, 644, 717, 1080, 1140, 1403, 1471
BstDEI CTNAG 4 cut(s) 108, 975, 1236, 1249
BstDSI CCRYGG 2 cut(s) 927, 1530
BstF5I GGATG 5 cut(s) 125, 149, 273, 574, 1372
BstFNI CGCG 9 cut(s) 260, 347, 391, 407, 422, 472, 642, 648, 1019
BstH2I RGCGCY 2 cut(s) 584, 1402
BstHHI GCGC 5 cut(s) 424, 472, 583, 648, 1401
BstKTI GATC 8 cut(s) 83, 162, 198, 378, 444, 922, 1051, 1323
BstMAI GTCTC 2 cut(s) 284, 878
BstMBI GATC 8 cut(s) 80, 159, 195, 375, 441, 919, 1048, 1320
BstMCI CGRYCG 3 cut(s) 44, 60, 169
BstMWI GCNNNNNNNGC 5 cut(s) 356, 580, 857, 1016, 1466
BstNSI RCATGY 1 cut(s) 719
BstPAI GACNNNNGTC 1 cut(s) 296
BstSCI CCNGG 2 cut(s) 54, 213
BstSFI CTRYAG 1 cut(s) 560
BstSLI GKGCMC 1 cut(s) 576
BstUI CGCG 9 cut(s) 260, 347, 391, 407, 422, 472, 642, 648, 1019
BstV1I GCAGC 2 cut(s) 546, 835
BstX2I RGATCY 2 cut(s) 919, 1048
BstYI RGATCY 2 cut(s) 919, 1048
BstZI CGGCCG 2 cut(s) 41, 57
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 1198
BsuRI GGCC 7 cut(s) 43, 59, 176, 318, 329, 350, 1262
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 2 cut(s) 927, 1530
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 1 cut(s) 1318
BtsCI GGATG 5 cut(s) 125, 149, 273, 574, 1372
BtuMI TCGCGA 1 cut(s) 642
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 8 cut(s) 34, 320, 644, 717, 1080, 1140, 1403, 1471
CaiI CAGNNNCTG 2 cut(s) 860, 1313
CfoI GCGC 5 cut(s) 424, 472, 583, 648, 1401
Cfr13I GGNCC 7 cut(s) 217, 262, 349, 453, 761, 1535, 1578
ClaI ATCGAT 1 cut(s) 378
CseI GACGC 3 cut(s) 413, 461, 1581
Csp6I GTAC 4 cut(s) 77, 235, 520, 733
CviAII CATG 8 cut(s) 439, 499, 716, 869, 1075, 1095, 1126, 1531
CviQI GTAC 4 cut(s) 77, 235, 520, 733
DdeI CTNAG 4 cut(s) 108, 975, 1236, 1249
DinI GGCGCC 1 cut(s) 1400
DpnI GATC 8 cut(s) 82, 161, 197, 377, 443, 921, 1050, 1322
DpnII GATC 8 cut(s) 80, 159, 195, 375, 441, 919, 1048, 1320
DriI GACNNNNNGTC 1 cut(s) 463
EaeI YGGCCR 4 cut(s) 41, 57, 174, 327
EagI CGGCCG 2 cut(s) 41, 57
Eam1105I GACNNNNNGTC 1 cut(s) 463
EclXI CGGCCG 2 cut(s) 41, 57
Eco130I CCWWGG 3 cut(s) 552, 576, 1530
Eco32I GATATC 1 cut(s) 601
Eco47I GGWCC 6 cut(s) 217, 262, 453, 761, 1535, 1578
Eco52I CGGCCG 2 cut(s) 41, 57
Eco57I CTGAAG 2 cut(s) 837, 1407
Eco72I CACGTG 1 cut(s) 296
Eco88I CYCGRG 5 cut(s) 206, 220, 250, 287, 479
EcoO109I RGGNCCY 1 cut(s) 1535
EcoRV GATATC 1 cut(s) 601
EcoT14I CCWWGG 3 cut(s) 552, 576, 1530
EgeI GGCGCC 1 cut(s) 1400
EheI GGCGCC 1 cut(s) 1400
ErhI CCWWGG 3 cut(s) 552, 576, 1530
FaeI CATG 8 cut(s) 442, 502, 719, 872, 1078, 1098, 1129, 1534
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FaqI GGGAC 1 cut(s) 466
FatI CATG 8 cut(s) 438, 498, 715, 868, 1074, 1094, 1125, 1530
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 1414
Fnu4HI GCNGC 5 cut(s) 44, 60, 348, 560, 849
FokI GGATG 5 cut(s) 112, 136, 260, 581, 1379
Fsp4HI GCNGC 5 cut(s) 44, 60, 348, 560, 849
FspBI CTAG 3 cut(s) 743, 933, 1449
GlaI GCGC 5 cut(s) 423, 471, 582, 647, 1400
GluI GCNGC 5 cut(s) 44, 60, 348, 560, 849
GsaI CCCAGC 1 cut(s) 387
HaeII RGCGCY 2 cut(s) 584, 1402
HaeIII GGCC 7 cut(s) 43, 59, 176, 318, 329, 350, 1262
HapII CCGG 4 cut(s) 56, 215, 1241, 1576
HgaI GACGC 3 cut(s) 413, 461, 1581
HhaI GCGC 5 cut(s) 424, 472, 583, 648, 1401
Hin1I GRCGYC 3 cut(s) 183, 1399, 1573
Hin1II CATG 8 cut(s) 442, 502, 719, 872, 1078, 1098, 1129, 1534
Hin6I GCGC 5 cut(s) 422, 470, 581, 646, 1399
HinP1I GCGC 5 cut(s) 422, 470, 581, 646, 1399
HinfI GANTC 4 cut(s) 110, 335, 1055, 1425
HpaII CCGG 4 cut(s) 56, 215, 1241, 1576
HphI GGTGA 3 cut(s) 87, 105, 292
Hpy166II GTNNAC 2 cut(s) 522, 903
Hpy188I TCNGA 6 cut(s) 568, 976, 983, 1135, 1309, 1571
Hpy8I GTNNAC 2 cut(s) 522, 903
Hpy99I CGWCG 4 cut(s) 42, 107, 368, 407
HpyAV CCTTC 2 cut(s) 966, 1367
HpyCH4III ACNGT 4 cut(s) 70, 364, 632, 889
HpyCH4IV ACGT 4 cut(s) 183, 295, 1317, 1483
HpyF10VI GCNNNNNNNGC 5 cut(s) 356, 580, 857, 1016, 1466
HpyF3I CTNAG 4 cut(s) 108, 975, 1236, 1249
HpySE526I ACGT 4 cut(s) 183, 295, 1317, 1483
Hsp92I GRCGYC 3 cut(s) 183, 1399, 1573
Hsp92II CATG 8 cut(s) 442, 502, 719, 872, 1078, 1098, 1129, 1534
HspAI GCGC 5 cut(s) 422, 470, 581, 646, 1399
KasI GGCGCC 1 cut(s) 1398
Kzo9I GATC 8 cut(s) 80, 159, 195, 375, 441, 919, 1048, 1320
LmnI GCTCC 5 cut(s) 433, 532, 857, 1034, 1359
Lsp1109I GCAGC 2 cut(s) 546, 835
LweI GCATC 3 cut(s) 370, 1046, 1447
MaeI CTAG 3 cut(s) 743, 933, 1449
MaeII ACGT 4 cut(s) 183, 295, 1317, 1483
MaeIII GTNAC 4 cut(s) 111, 298, 506, 995
MalI GATC 8 cut(s) 82, 161, 197, 377, 443, 921, 1050, 1322
MbiI CCGCTC 1 cut(s) 62
MboI GATC 8 cut(s) 80, 159, 195, 375, 441, 919, 1048, 1320
MboII GAAGA 8 cut(s) 205, 436, 629, 791, 884, 1338, 1400, 1434
MfeI CAATTG 1 cut(s) 1521
MflI RGATCY 2 cut(s) 919, 1048
MhlI GDGCHC 3 cut(s) 438, 576, 1330
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 5 cut(s) 908, 1289, 1439, 1452, 1521
MluNI TGGCCA 1 cut(s) 329
Mly113I GGCGCC 1 cut(s) 1399
MlyI GAGTC 3 cut(s) 119, 344, 1049
MmeI TCCRAC 2 cut(s) 546, 947
Mox20I TGGCCA 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 798
MscI TGGCCA 1 cut(s) 329
MseI TTAA 3 cut(s) 777, 891, 1464
MslI CAYNNNNRTG 3 cut(s) 513, 714, 1365
Msp20I TGGCCA 1 cut(s) 329
MspI CCGG 4 cut(s) 56, 215, 1241, 1576
MspR9I CCNGG 2 cut(s) 56, 215
MunI CAATTG 1 cut(s) 1521
Mva1269I GAATGC 3 cut(s) 520, 725, 1025
MvnI CGCG 9 cut(s) 260, 347, 391, 407, 422, 472, 642, 648, 1019
MwoI GCNNNNNNNGC 5 cut(s) 356, 580, 857, 1016, 1466
NarI GGCGCC 1 cut(s) 1399
NciI CCSGG 2 cut(s) 56, 215
NcoI CCATGG 1 cut(s) 1530
NdeI CATATG 1 cut(s) 1414
NdeII GATC 8 cut(s) 80, 159, 195, 375, 441, 919, 1048, 1320
NlaIII CATG 8 cut(s) 442, 502, 719, 872, 1078, 1098, 1129, 1534
NlaIV GGNNCC 7 cut(s) 123, 219, 227, 418, 454, 1400, 1536
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 4 cut(s) 111, 298, 506, 995
NruI TCGCGA 1 cut(s) 642
NspI RCATGY 1 cut(s) 719
PaeI GCATGC 1 cut(s) 719
PaeR7I CTCGAG 2 cut(s) 206, 479
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PctI GAATGC 3 cut(s) 520, 725, 1025
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 1 cut(s) 1425
Pfl23II CGTACG 1 cut(s) 76
PkrI GCNGC 5 cut(s) 45, 61, 349, 561, 850
PleI GAGTC 3 cut(s) 118, 343, 1049
PluTI GGCGCC 1 cut(s) 1402
PmaCI CACGTG 1 cut(s) 296
PmlI CACGTG 1 cut(s) 296
PpsI GAGTC 3 cut(s) 118, 343, 1049
Ppu21I YACGTR 1 cut(s) 296
PpuMI RGGWCCY 1 cut(s) 1535
PshAI GACNNNNGTC 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 1535
PspCI CACGTG 1 cut(s) 296
PspFI CCCAGC 1 cut(s) 383
PspLI CGTACG 1 cut(s) 76
PspN4I GGNNCC 7 cut(s) 123, 219, 227, 418, 454, 1400, 1536
PspPI GGNCC 7 cut(s) 217, 262, 349, 453, 761, 1535, 1578
PspPPI RGGWCCY 1 cut(s) 1535
PspXI VCTCGAGB 1 cut(s) 479
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 2 cut(s) 860, 1313
PsuI RGATCY 2 cut(s) 919, 1048
RruI TCGCGA 1 cut(s) 642
RsaI GTAC 4 cut(s) 78, 236, 521, 734
RsaNI GTAC 4 cut(s) 77, 235, 520, 733
RseI CAYNNNNRTG 3 cut(s) 513, 714, 1365
SaqAI TTAA 3 cut(s) 777, 891, 1464
SatI GCNGC 5 cut(s) 44, 60, 348, 560, 849
Sau3AI GATC 8 cut(s) 80, 159, 195, 375, 441, 919, 1048, 1320
Sau96I GGNCC 7 cut(s) 217, 262, 349, 453, 761, 1535, 1578
ScaI AGTACT 1 cut(s) 734
SchI GAGTC 3 cut(s) 119, 344, 1049
ScrFI CCNGG 2 cut(s) 56, 215
SduI GDGCHC 3 cut(s) 438, 576, 1330
SfaNI GCATC 3 cut(s) 370, 1046, 1447
SfcI CTRYAG 1 cut(s) 560
SfoI GGCGCC 1 cut(s) 1400
Sfr274I CTCGAG 2 cut(s) 206, 479
SinI GGWCC 6 cut(s) 217, 262, 453, 761, 1535, 1578
SlaI CTCGAG 2 cut(s) 206, 479
SmiMI CAYNNNNRTG 3 cut(s) 513, 714, 1365
SmlI CTYRAG 3 cut(s) 206, 230, 479
SmoI CTYRAG 3 cut(s) 206, 230, 479
SphI GCATGC 1 cut(s) 719
Sse9I AATT 5 cut(s) 908, 1289, 1439, 1452, 1521
SspDI GGCGCC 1 cut(s) 1398
SspI AATATT 1 cut(s) 775
SspMI CTAG 3 cut(s) 743, 933, 1449
StyD4I CCNGG 2 cut(s) 54, 213
StyI CCWWGG 3 cut(s) 552, 576, 1530
TaaI ACNGT 4 cut(s) 70, 364, 632, 889
TaiI ACGT 4 cut(s) 186, 298, 1320, 1486
TaqII GACCGA 1 cut(s) 470
TasI AATT 5 cut(s) 908, 1289, 1439, 1452, 1521
TatI WGTACW 1 cut(s) 732
TauI GCSGC 3 cut(s) 46, 62, 350
TfiI GAWTC 1 cut(s) 1425
Tru1I TTAA 3 cut(s) 777, 891, 1464
Tru9I TTAA 3 cut(s) 777, 891, 1464
TseFI GTSAC 4 cut(s) 111, 298, 506, 995
TseI GCWGC 2 cut(s) 559, 848
Tsp45I GTSAC 4 cut(s) 111, 298, 506, 995
TspDTI ATGAA 5 cut(s) 23, 708, 885, 1083, 1293
TspGWI ACGGA 1 cut(s) 1313
VpaK11BI GGWCC 6 cut(s) 217, 262, 453, 761, 1535, 1578
XceI RCATGY 1 cut(s) 719
XhoI CTCGAG 2 cut(s) 206, 479
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 3 cut(s) 743, 933, 1449
ZraI GACGTC 1 cut(s) 184
ZrmI AGTACT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.