RLG00000021590

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015224
Physical Location & Seq
Reverse (-)
76631479 .. 76634728
3250 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021590

Sequence Viewer

Length: 1596 bp
ATGGCGATGAAGCACCTGCTCACAGCGCTGGCCCGCCGCCGGTCGGTCAAGGCCTCACCGGCGCTGGCCCAAGCCGCCCGATCCAGCTCCACCTCACCCGCCGTCGCACCTTCTCTCTCAACCCCACTGCCCAGGCCTCCTCCGCCGACCGCCATGGTCTACGATCGCCTCGCCGAGGACGTCAAATCCAAGCTCCAGAGGCTCGAGAACCCCGACCCGAGGTTCCTCAAGTACGGGTCGCCCCACCCGACCCTGACGGATCATTCGCACATCCTCACCGCCCCGGAGACACGCGTCACCGTCCTCCCCAACGGTTTGAGAGTCGCGACGGAGTCGAACCTCGCCGCGAAGACCGCGACCGTCGGGGTCTGGATCGACTCGGGGTCGAGATTCGAGAACGATGAGAACAACGGCACGGCGCATTTCCTGGAGCACATGATCTTCAAGGGGACGGAGAAGAGGACGGCGCTGGATCTGGAGGTGGAGGTGGAGAACATGGGAGGGCACTTGAATGCTTATACCTCCAGGGAGCAGACGACGTACTACGCCAAGGTGCTCGATAAGGACGTGCCCCAAGCATTGTCTATTTTGGCTGATATCTTGCAGAACTCGAGGTTCGATGAGAACCGGATTAGCAGAGAGCGTGATGTGATTTTGAGGGAAATGGAGGAGGTTGAGAAGCAACCTGATGAAGTCGTTTTTGACCATCTACATGCAAGTGCATTCCAGTACTCTCCTTTGGCCAGAACTATACTTGGTCCTGCTGCAAACATTAAGACTATTTCCAAAGAGCATCTTCAGAATTATATTAAAACACACTACACAGCTCCCAGAATGGTGATTGCTGCTTCAGGAGCTATTAAGCATGAAGACCTTGTTGGGCAAGTTAAGGGTTTATTTACAAATTTATCAGCTGATCCAACGACAGCTAGTCAGTTGGTTGCAAAAGAACCTTCATTTTTCACCGGTTCTGAGGTCAGAATTGTTGATGATGACTTGCCTTATGCAAACTTTGCTGTTGCATTCAGTGGAGCATCCTGGACAGATCCAGACTCTATTGCTCTAATGGTCATGCAGGCAATGTTGGGTTCATGGAGTAAAAGTGTCAATGGTGGAAAGCACATGGGTTCTGAGTTGACGCAGAGGGTTGCCATTAACGAAATAGCAGAAAGCATAATGGCTTTCAACACCAACTACAAGGACACTGGCTTATTCGGTGTTTTTGCTACTGCAAAGCCGGATGCTTTGGATGATTTGGCCTATGCAATAATGTATGAGATAACCAAGTTAGTTTATCGAGTTTCTGAAGCTGATGTCATTCGTGCTCGCAATCAGTTAAAATCTTCTCTCCTTCTTCACTTGGATGGGACAAGTCCTGTAGCTGAAGATATTGGGCGCCAGCTACTCACATATGGTCGAAGAATCCCATTGCCCGAGTTGTTTGCTAGAATTGATGCTGTTGATGCAAGCACCGTCAAACGGGTTGCAAACCGGTTTATCTATGACAGGGATGTTGCAATTGTTGCCATGGGTCCTGTTCAGAAATTGCCCGACTACAACTGGTTCAGACGCAGGACATACTGGAACAGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

532

Amino Acids

59.03

Weight (kDa)

6.72

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 107 - 254 1.9e-56 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 261 - 446 2.2e-37 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 24
AatII GACGTC 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 1395
AccI GTMKAC 1 cut(s) 159
AccII CGCG 4 cut(s) 294, 326, 347, 356
AciI CCGC 9 cut(s) 34, 37, 75, 99, 143, 150, 279, 345, 354
AclWI GGATC 6 cut(s) 75, 267, 380, 480, 911, 1040
AcoI YGGCCR 1 cut(s) 741
AcsI RAATTY 1 cut(s) 904
AcuI CTGAAG 4 cut(s) 782, 834, 1326, 1404
AcyI GRCGYC 2 cut(s) 180, 1396
AfaI GTAC 3 cut(s) 233, 542, 731
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 5 cut(s) 39, 43, 175, 219, 1479
AflIII ACRYGT 1 cut(s) 292
AgeI ACCGGT 2 cut(s) 965, 1491
AgsI TTSAA 3 cut(s) 445, 511, 1186
AhdI GACNNNNNGTC 2 cut(s) 382, 930
AjiI CACGTC 1 cut(s) 568
AjnI CCWGG 4 cut(s) 131, 426, 524, 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 861, 893
AluBI AGCT 9 cut(s) 87, 193, 827, 857, 914, 929, 1310, 1382, 1402
AluI AGCT 9 cut(s) 87, 193, 827, 857, 914, 929, 1310, 1382, 1402
Alw21I GWGCWC 3 cut(s) 435, 558, 1327
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 6 cut(s) 75, 267, 380, 480, 911, 1040
Ama87I CYCGRG 5 cut(s) 203, 217, 379, 610, 1433
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 6 cut(s) 30, 51, 66, 134, 741, 1257
ApeKI GCWGC 2 cut(s) 764, 845
ApoI RAATTY 1 cut(s) 904
ArsI GACNNNNNNTTYG 2 cut(s) 247, 279
AsiGI ACCGGT 2 cut(s) 965, 1491
AspLEI GCGC 5 cut(s) 28, 64, 421, 469, 1398
AspS9I GGNCC 4 cut(s) 31, 67, 758, 1532
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 6 cut(s) 48, 87, 268, 289, 850, 955
AvaI CYCGRG 5 cut(s) 203, 217, 379, 610, 1433
AvaII GGWCC 2 cut(s) 758, 1532
BaeGI GKGCMC 2 cut(s) 507, 573
BalI TGGCCA 1 cut(s) 743
BanI GGYRCC 1 cut(s) 1395
BbsI GAAGAC 2 cut(s) 356, 876
Bbv12I GWGCWC 3 cut(s) 435, 558, 1327
BbvI GCAGC 2 cut(s) 751, 832
BccI CCATC 2 cut(s) 714, 1358
BceAI ACGGC 4 cut(s) 86, 427, 432, 480
BcgI CGANNNNNNTGC 2 cut(s) 1424, 1458
BciT130I CCWGG 4 cut(s) 133, 428, 526, 1039
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 3 cut(s) 930, 1446, 1594
BfmI CTRYAG 1 cut(s) 1377
BfoI RGCGCY 4 cut(s) 29, 65, 470, 1399
BfuAI ACCTGC 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 5 cut(s) 37, 75, 345, 765, 846
BlsI GCNGC 5 cut(s) 38, 76, 346, 766, 847
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 5 cut(s) 133, 284, 428, 526, 1039
Bme18I GGWCC 2 cut(s) 758, 1532
BmeRI GACNNNNNGTC 2 cut(s) 382, 930
BmeT110I CYCGRG 5 cut(s) 203, 217, 379, 610, 1433
BmgBI CACGTC 1 cut(s) 568
BmgT120I GGNCC 4 cut(s) 31, 67, 758, 1532
BmiI GGNNCC 3 cut(s) 224, 1397, 1533
BmrFI CCNGG 5 cut(s) 133, 284, 428, 526, 1039
BmsI GCATC 5 cut(s) 802, 1043, 1231, 1444, 1453
BoxI GACNNNNGTC 1 cut(s) 293
BpiI GAAGAC 2 cut(s) 356, 876
BpmI CTGGAG 4 cut(s) 179, 449, 497, 508
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 284
BsaHI GRCGYC 2 cut(s) 180, 1396
BsaJI CCNNGG 8 cut(s) 131, 153, 174, 218, 282, 525, 549, 1527
BsaWI WCCGGW 3 cut(s) 627, 965, 1491
BsaXI ACNNNNNCTCC 2 cut(s) 1087, 1117
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 43, 175, 219, 1479
Bse118I RCCGGY 4 cut(s) 39, 58, 965, 1491
Bse1I ACTGG 4 cut(s) 727, 1210, 1565, 1586
Bse3DI GCAATG 2 cut(s) 1086, 1427
BseBI CCWGG 4 cut(s) 133, 428, 526, 1039
BseDI CCNNGG 8 cut(s) 131, 153, 174, 218, 282, 525, 549, 1527
BseGI GGATG 6 cut(s) 270, 1034, 1246, 1255, 1369, 1516
BseLI CCNNNNNNNGG 5 cut(s) 39, 43, 175, 219, 1479
BseMI GCAATG 2 cut(s) 1086, 1427
BseMII CTCAG 2 cut(s) 963, 1122
BseNI ACTGG 4 cut(s) 727, 1210, 1565, 1586
BseRI GAGGAG 2 cut(s) 129, 683
BseSI GKGCMC 2 cut(s) 507, 573
BseXI GCAGC 2 cut(s) 751, 832
Bsh1236I CGCG 4 cut(s) 294, 326, 347, 356
Bsh1285I CGRYCG 4 cut(s) 44, 150, 166, 360
BshFI GGCC 6 cut(s) 32, 53, 68, 136, 743, 1259
BshNI GGYRCC 1 cut(s) 1395
BshTI ACCGGT 2 cut(s) 965, 1491
BsiEI CGRYCG 4 cut(s) 44, 150, 166, 360
BsiHKAI GWGCWC 3 cut(s) 435, 558, 1327
BsiHKCI CYCGRG 5 cut(s) 203, 217, 379, 610, 1433
BsiSI CCGG 7 cut(s) 40, 59, 284, 628, 966, 1238, 1492
BslFI GGGAC 2 cut(s) 463, 1381
BslI CCNNNNNNNGG 5 cut(s) 39, 43, 175, 219, 1479
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 2 cut(s) 463, 1381
BsmI GAATGC 3 cut(s) 517, 722, 1022
BsnI GGCC 6 cut(s) 32, 53, 68, 136, 743, 1259
BsoBI CYCGRG 5 cut(s) 203, 217, 379, 610, 1433
Bsp1286I GDGCHC 5 cut(s) 435, 507, 558, 573, 1327
Bsp143I GATC 8 cut(s) 80, 163, 259, 372, 438, 472, 916, 1045
Bsp19I CCATGG 2 cut(s) 153, 1527
Bsp68I TCGCGA 1 cut(s) 326
BspACI CCGC 9 cut(s) 34, 37, 75, 99, 143, 150, 279, 345, 354
BspANI GGCC 6 cut(s) 32, 53, 68, 136, 743, 1259
BspCNI CTCAG 2 cut(s) 964, 1123
BspFNI CGCG 4 cut(s) 294, 326, 347, 356
BspLI GGNNCC 3 cut(s) 224, 1397, 1533
BspMI ACCTGC 1 cut(s) 24
BspPI GGATC 6 cut(s) 75, 267, 380, 480, 911, 1040
BspT107I GGYRCC 1 cut(s) 1395
BsrDI GCAATG 2 cut(s) 1086, 1427
BsrFI RCCGGY 4 cut(s) 39, 58, 965, 1491
BsrI ACTGG 4 cut(s) 727, 1210, 1565, 1586
BssAI RCCGGY 4 cut(s) 39, 58, 965, 1491
BssECI CCNNGG 8 cut(s) 131, 153, 174, 218, 282, 525, 549, 1527
BssMI GATC 8 cut(s) 80, 163, 259, 372, 438, 472, 916, 1045
BssNI GRCGYC 2 cut(s) 180, 1396
BssT1I CCWWGG 3 cut(s) 153, 549, 1527
Bst2UI CCWGG 4 cut(s) 133, 428, 526, 1039
Bst4CI ACNGT 4 cut(s) 301, 314, 361, 1474
Bst6I CTCTTC 1 cut(s) 452
BstACI GRCGYC 2 cut(s) 180, 1396
BstAPI GCANNNNNTGC 2 cut(s) 1013, 1523
BstC8I GCNNGC 7 cut(s) 30, 34, 66, 1077, 1327, 1400, 1468
BstDEI CTNAG 2 cut(s) 972, 1131
BstDSI CCRYGG 2 cut(s) 153, 1527
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 6 cut(s) 270, 1034, 1246, 1255, 1369, 1516
BstFNI CGCG 4 cut(s) 294, 326, 347, 356
BstH2I RGCGCY 4 cut(s) 29, 65, 470, 1399
BstHHI GCGC 5 cut(s) 28, 64, 421, 469, 1398
BstKTI GATC 8 cut(s) 83, 166, 262, 375, 441, 475, 919, 1048
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 8 cut(s) 80, 163, 259, 372, 438, 472, 916, 1045
BstMCI CGRYCG 4 cut(s) 44, 150, 166, 360
BstNI CCWGG 4 cut(s) 133, 428, 526, 1039
BstNSI RCATGY 1 cut(s) 716
BstPAI GACNNNNGTC 1 cut(s) 293
BstSCI CCNGG 5 cut(s) 131, 282, 426, 524, 1037
BstSFI CTRYAG 1 cut(s) 1377
BstSLI GKGCMC 2 cut(s) 507, 573
BstUI CGCG 4 cut(s) 294, 326, 347, 356
BstV1I GCAGC 2 cut(s) 751, 832
BstV2I GAAGAC 2 cut(s) 356, 876
BstX2I RGATCY 2 cut(s) 472, 1045
BstYI RGATCY 2 cut(s) 472, 1045
BsuRI GGCC 6 cut(s) 32, 53, 68, 136, 743, 1259
BtgI CCRYGG 2 cut(s) 153, 1527
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 1 cut(s) 568
BtsCI GGATG 6 cut(s) 270, 1034, 1246, 1255, 1369, 1516
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 3 cut(s) 125, 1033, 1203
BtuMI TCGCGA 1 cut(s) 326
BveI ACCTGC 1 cut(s) 24
Cac8I GCNNGC 7 cut(s) 30, 34, 66, 1077, 1327, 1400, 1468
CfoI GCGC 5 cut(s) 28, 64, 421, 469, 1398
Cfr10I RCCGGY 4 cut(s) 39, 58, 965, 1491
Cfr13I GGNCC 4 cut(s) 31, 67, 758, 1532
CseI GACGC 3 cut(s) 283, 1147, 1578
Csp6I GTAC 3 cut(s) 232, 541, 730
CspAI ACCGGT 2 cut(s) 965, 1491
CviAII CATG 9 cut(s) 154, 436, 496, 713, 866, 1072, 1092, 1123, 1528
CviQI GTAC 3 cut(s) 232, 541, 730
DdeI CTNAG 2 cut(s) 972, 1131
DinI GGCGCC 1 cut(s) 1397
DpnI GATC 8 cut(s) 82, 165, 261, 374, 440, 474, 918, 1047
DpnII GATC 8 cut(s) 80, 163, 259, 372, 438, 472, 916, 1045
DriI GACNNNNNGTC 2 cut(s) 382, 930
EaeI YGGCCR 1 cut(s) 741
Eam1104I CTCTTC 1 cut(s) 452
Eam1105I GACNNNNNGTC 2 cut(s) 382, 930
EarI CTCTTC 1 cut(s) 452
EciI GGCGGA 1 cut(s) 132
Eco130I CCWWGG 3 cut(s) 153, 549, 1527
Eco147I AGGCCT 2 cut(s) 53, 136
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 2 cut(s) 758, 1532
Eco47III AGCGCT 1 cut(s) 27
Eco57I CTGAAG 4 cut(s) 782, 834, 1326, 1404
Eco88I CYCGRG 5 cut(s) 203, 217, 379, 610, 1433
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 1532
EcoRII CCWGG 4 cut(s) 131, 426, 524, 1037
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 3 cut(s) 153, 549, 1527
EgeI GGCGCC 1 cut(s) 1397
EheI GGCGCC 1 cut(s) 1397
ErhI CCWWGG 3 cut(s) 153, 549, 1527
FaeI CATG 9 cut(s) 157, 439, 499, 716, 869, 1075, 1095, 1126, 1531
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 2 cut(s) 463, 1381
FatI CATG 9 cut(s) 153, 435, 495, 712, 865, 1071, 1091, 1122, 1527
FauI CCCGC 2 cut(s) 41, 106
FauNDI CATATG 1 cut(s) 1411
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 5 cut(s) 37, 75, 345, 765, 846
FokI GGATG 6 cut(s) 257, 1021, 1253, 1262, 1376, 1523
Fsp4HI GCNGC 5 cut(s) 37, 75, 345, 765, 846
FspBI CTAG 3 cut(s) 930, 1446, 1594
GlaI GCGC 5 cut(s) 27, 63, 420, 468, 1397
GluI GCNGC 5 cut(s) 37, 75, 345, 765, 846
GsuI CTGGAG 4 cut(s) 179, 449, 497, 508
HaeII RGCGCY 4 cut(s) 29, 65, 470, 1399
HaeIII GGCC 6 cut(s) 32, 53, 68, 136, 743, 1259
HapII CCGG 7 cut(s) 40, 59, 284, 628, 966, 1238, 1492
HgaI GACGC 3 cut(s) 283, 1147, 1578
HhaI GCGC 5 cut(s) 28, 64, 421, 469, 1398
Hin1I GRCGYC 2 cut(s) 180, 1396
Hin1II CATG 9 cut(s) 157, 439, 499, 716, 869, 1075, 1095, 1126, 1531
Hin6I GCGC 5 cut(s) 26, 62, 419, 467, 1396
HinP1I GCGC 5 cut(s) 26, 62, 419, 467, 1396
HincII GTYRAC 1 cut(s) 1137
HindII GTYRAC 1 cut(s) 1137
HinfI GANTC 6 cut(s) 321, 332, 377, 390, 1052, 1422
HpaII CCGG 7 cut(s) 40, 59, 284, 628, 966, 1238, 1492
HphI GGTGA 6 cut(s) 48, 87, 268, 289, 850, 955
Hpy166II GTNNAC 2 cut(s) 160, 1137
Hpy188I TCNGA 7 cut(s) 801, 973, 980, 1132, 1306, 1542, 1568
Hpy188III TCNNGA 9 cut(s) 196, 205, 325, 370, 387, 394, 476, 852, 1049
Hpy8I GTNNAC 2 cut(s) 160, 1137
Hpy99I CGWCG 4 cut(s) 107, 331, 365, 541
HpyAV CCTTC 3 cut(s) 120, 963, 1361
HpyCH4III ACNGT 4 cut(s) 301, 314, 361, 1474
HpyCH4IV ACGT 3 cut(s) 180, 539, 567
HpyF3I CTNAG 2 cut(s) 972, 1131
HpySE526I ACGT 3 cut(s) 180, 539, 567
Hsp92I GRCGYC 2 cut(s) 180, 1396
Hsp92II CATG 9 cut(s) 157, 439, 499, 716, 869, 1075, 1095, 1126, 1531
HspAI GCGC 5 cut(s) 26, 62, 419, 467, 1396
KasI GGCGCC 1 cut(s) 1395
Kzo9I GATC 8 cut(s) 80, 163, 259, 372, 438, 472, 916, 1045
LmnI GCTCC 7 cut(s) 92, 198, 430, 529, 832, 854, 1031
Lsp1109I GCAGC 2 cut(s) 751, 832
LweI GCATC 5 cut(s) 802, 1043, 1231, 1444, 1453
MaeI CTAG 3 cut(s) 930, 1446, 1594
MaeII ACGT 3 cut(s) 180, 539, 567
MaeIII GTNAC 1 cut(s) 295
MalI GATC 8 cut(s) 82, 165, 261, 374, 440, 474, 918, 1047
MboI GATC 8 cut(s) 80, 163, 259, 372, 438, 472, 916, 1045
MboII GAAGA 9 cut(s) 361, 433, 469, 788, 881, 1335, 1346, 1397, 1431
MfeI CAATTG 1 cut(s) 1518
MflI RGATCY 2 cut(s) 472, 1045
MhlI GDGCHC 5 cut(s) 435, 507, 558, 573, 1327
MlsI TGGCCA 1 cut(s) 743
MluCI AATT 6 cut(s) 802, 904, 981, 1449, 1518, 1544
MluI ACGCGT 1 cut(s) 292
MluNI TGGCCA 1 cut(s) 743
Mly113I GGCGCC 1 cut(s) 1396
MlyI GAGTC 4 cut(s) 330, 341, 371, 1046
MmeI TCCRAC 1 cut(s) 944
Mox20I TGGCCA 1 cut(s) 743
MscI TGGCCA 1 cut(s) 743
MseI TTAA 6 cut(s) 774, 810, 861, 888, 1155, 1337
MslI CAYNNNNRTG 4 cut(s) 510, 711, 717, 1362
Msp20I TGGCCA 1 cut(s) 743
MspA1I CMGCKG 1 cut(s) 914
MspI CCGG 7 cut(s) 40, 59, 284, 628, 966, 1238, 1492
MspR9I CCNGG 5 cut(s) 133, 284, 428, 526, 1039
MunI CAATTG 1 cut(s) 1518
Mva1269I GAATGC 3 cut(s) 517, 722, 1022
MvaI CCWGG 4 cut(s) 133, 428, 526, 1039
MvnI CGCG 4 cut(s) 294, 326, 347, 356
NarI GGCGCC 1 cut(s) 1396
NciI CCSGG 1 cut(s) 284
NcoI CCATGG 2 cut(s) 153, 1527
NdeI CATATG 1 cut(s) 1411
NdeII GATC 8 cut(s) 80, 163, 259, 372, 438, 472, 916, 1045
NlaIII CATG 9 cut(s) 157, 439, 499, 716, 869, 1075, 1095, 1126, 1531
NlaIV GGNNCC 3 cut(s) 224, 1397, 1533
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 295
NruI TCGCGA 1 cut(s) 326
NspI RCATGY 1 cut(s) 716
PaeR7I CTCGAG 2 cut(s) 203, 610
PaqCI CACCTGC 1 cut(s) 24
PceI AGGCCT 2 cut(s) 53, 136
PcsI WCGNNNNNNNCGW 4 cut(s) 177, 210, 245, 564
PctI GAATGC 3 cut(s) 517, 722, 1022
PfeI GAWTC 2 cut(s) 390, 1422
PflFI GACNNNGTC 1 cut(s) 331
PfoI TCCNGGA 2 cut(s) 426, 1037
PinAI ACCGGT 2 cut(s) 965, 1491
PkrI GCNGC 5 cut(s) 38, 76, 346, 766, 847
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 4 cut(s) 329, 340, 371, 1046
PluTI GGCGCC 1 cut(s) 1399
PpsI GAGTC 4 cut(s) 329, 340, 371, 1046
PpuMI RGGWCCY 1 cut(s) 1532
PshAI GACNNNNGTC 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 1532
Psp6I CCWGG 4 cut(s) 131, 426, 524, 1037
PspGI CCWGG 4 cut(s) 131, 426, 524, 1037
PspN4I GGNNCC 3 cut(s) 224, 1397, 1533
PspPI GGNCC 4 cut(s) 31, 67, 758, 1532
PspPPI RGGWCCY 1 cut(s) 1532
PspXI VCTCGAGB 1 cut(s) 610
PsuI RGATCY 2 cut(s) 472, 1045
PsyI GACNNNGTC 1 cut(s) 331
PvuI CGATCG 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 914
RruI TCGCGA 1 cut(s) 326
RsaI GTAC 3 cut(s) 233, 542, 731
RsaNI GTAC 3 cut(s) 232, 541, 730
RseI CAYNNNNRTG 4 cut(s) 510, 711, 717, 1362
SaqAI TTAA 6 cut(s) 774, 810, 861, 888, 1155, 1337
SatI GCNGC 5 cut(s) 37, 75, 345, 765, 846
Sau3AI GATC 8 cut(s) 80, 163, 259, 372, 438, 472, 916, 1045
Sau96I GGNCC 4 cut(s) 31, 67, 758, 1532
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 4 cut(s) 330, 341, 371, 1046
ScrFI CCNGG 5 cut(s) 133, 284, 428, 526, 1039
SduI GDGCHC 5 cut(s) 435, 507, 558, 573, 1327
SfaNI GCATC 5 cut(s) 802, 1043, 1231, 1444, 1453
SfcI CTRYAG 1 cut(s) 1377
SfoI GGCGCC 1 cut(s) 1397
Sfr274I CTCGAG 2 cut(s) 203, 610
SgrAI CRCCGGYG 1 cut(s) 58
SinI GGWCC 2 cut(s) 758, 1532
SlaI CTCGAG 2 cut(s) 203, 610
SmiMI CAYNNNNRTG 4 cut(s) 510, 711, 717, 1362
SmlI CTYRAG 3 cut(s) 203, 227, 610
SmoI CTYRAG 3 cut(s) 203, 227, 610
Sse9I AATT 6 cut(s) 802, 904, 981, 1449, 1518, 1544
SseBI AGGCCT 2 cut(s) 53, 136
SsiI CCGC 9 cut(s) 34, 37, 75, 99, 143, 150, 279, 345, 354
SspDI GGCGCC 1 cut(s) 1395
SspMI CTAG 3 cut(s) 930, 1446, 1594
StuI AGGCCT 2 cut(s) 53, 136
StyD4I CCNGG 5 cut(s) 131, 282, 426, 524, 1037
StyI CCWWGG 3 cut(s) 153, 549, 1527
TaaI ACNGT 4 cut(s) 301, 314, 361, 1474
TaiI ACGT 3 cut(s) 183, 542, 570
TaqII GACCGA 1 cut(s) 34
TasI AATT 6 cut(s) 802, 904, 981, 1449, 1518, 1544
TatI WGTACW 1 cut(s) 729
TauI GCSGC 3 cut(s) 39, 77, 347
TfiI GAWTC 2 cut(s) 390, 1422
Tru1I TTAA 6 cut(s) 774, 810, 861, 888, 1155, 1337
Tru9I TTAA 6 cut(s) 774, 810, 861, 888, 1155, 1337
TscAI CASTG 3 cut(s) 132, 1033, 1210
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 2 cut(s) 764, 845
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 5 cut(s) 23, 705, 882, 945, 1080
TspGWI ACGGA 3 cut(s) 272, 344, 467
TspRI CASTG 3 cut(s) 132, 1033, 1210
Tth111I GACNNNGTC 1 cut(s) 331
VpaK11BI GGWCC 2 cut(s) 758, 1532
XagI CCTNNNNNAGG 1 cut(s) 173
XapI RAATTY 1 cut(s) 904
XceI RCATGY 1 cut(s) 716
XhoI CTCGAG 2 cut(s) 203, 610
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 3 cut(s) 930, 1446, 1594
ZraI GACGTC 1 cut(s) 181
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.