Rmu_sc0006218.1_g000030

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006218.1
OG0015224
Physical Location & Seq
Reverse (-)
138391 .. 142548
4158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006218.1_g000030.1.cds

Sequence Viewer

Length: 1992 bp
atggcgatgaagcacctgctcacagcgctggcccgccgccggtcggtcaaggcctcgccggcgctgacccaagccgtccgatccagctccacctcacccgccgtcgcatcctccctctcaaccccaccgcccaggcctcctccgccgaccgccatggtctacgatcgcctggccgaggacgtcaaatccaagctccagaggctcgagaaccccgacctgaggttcctcaagtacgggtcgccccacccgaccctgacggatcactcgcacatcctcaccgcgccggagacacgcgtcaccgtcctccccaacggtttgagagtcgcgacggagtcgaacctggccgcgaagaccgcgaccgtcggggtctggatcgactcggggtcgaggttcgagaacgatgagaacaacggcacggcgcatttcctggagcacatgatcttcaaagggacggagaagaggacggcgctggatctggaggtggaggtggagaacatgggagggcacttgaatgcttatacctctagggagcagacgacctactacgccaaggtgctcgataaggacgtgccccaggcattgtctatattggctgatatcttgcagaactcgaggttcgatgagaaccggattagccgagagcgtgacgtgattttgagggaaatggaggaggttgagaagcaacctgatgaagtcgtttttgaccatctacatgcaagtgcattccagtactctcctttggccagaactatacttgggcctgctgcaaacattaagactatttccaaagagcatcttcagaattatattaaaacacactacactgctcccagaatggtgattgctgcttcaggagctattaagcatgaagaccttgttgggcaagttaagggtttgtttacaaacttatcagctgatccaacgacagcaagtcagttggttgcaaaagaaccttcatttttcaccggttctgaggtcagaattgttgatgatgacttgccttatgcaaactttgctgttgcattcagtggagcatcctggacagatccagactctattgctctaatggtcatgcaagcaatgttgggttcatggagtaaaagtgtcaatggtggaaagcacatgggttctgagttgacgcagagggttgctattaacgaaatagcagaaagcataatggctttcaacaccaactacaaggacactggcttattcggcgtttttgcaactgcgaagccggaagctttggatgatttggcctatgcaataatgtatgagataaccaagttagtttatcgagtttctgaaactgatgtcattcgtgctcgcaatcagttaaaatcttctctccttcttcacttggatgggacaagtcctgtagctgaagatattgggcgccagctactcacatatggtcgaagaatcccattgcccgagttgtttgctagaattgatgctgttgatgcaagcaccgtcaaacgggttgcaaaccggtttatctatgacagggtaagactacaatctgcgataactgttgtgtgtaaaattgcccgactacaactggttcagacgcaggacatactggaacagatactagatgttcttctgtgcgttaattatttcatcatcatcaactttcatgatcgatttgatcttggaatattttattgcttattgtccaagttagtttatcgagtttctgaaactgatgtcattcgtgctcgcaatcagttaaaatcttctctccttcttcacttggatgggacaagtcctgtagctgaagatattgggcgccagctactcacatatggtcgaagaatcccattgcccgagttgtttgctagaattgatgctgttgatgcaagcaccgtcaaacgggttgcaaaccggtttatctatgacagggatattgcaattgttgccatgggtcctgttcagaaattgcccgactacaactggttcagacgcaggacatactggaacagatactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

663

Amino Acids

74.31

Weight (kDa)

7.75

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 24
AatII GACGTC 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 24
AccB1I GGYRCC 2 cut(s) 1395, 1791
AccI GTMKAC 1 cut(s) 159
AccII CGCG 5 cut(s) 281, 294, 326, 347, 356
AciI CCGC 9 cut(s) 34, 37, 99, 128, 143, 150, 279, 345, 354
AclWI GGATC 6 cut(s) 75, 267, 380, 480, 911, 1040
AcoI YGGCCR 3 cut(s) 171, 342, 741
AcuI CTGAAG 4 cut(s) 782, 834, 1404, 1800
AcyI GRCGYC 3 cut(s) 180, 1396, 1792
AfaI GTAC 2 cut(s) 233, 731
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 6 cut(s) 39, 43, 175, 219, 1479, 1875
AflIII ACRYGT 1 cut(s) 292
AgeI ACCGGT 3 cut(s) 965, 1491, 1887
AgsI TTSAA 3 cut(s) 445, 511, 1186
AhdI GACNNNNNGTC 2 cut(s) 382, 930
AjiI CACGTC 2 cut(s) 568, 649
AjnI CCWGG 6 cut(s) 131, 168, 339, 426, 573, 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 861, 893
AluBI AGCT 9 cut(s) 87, 193, 857, 914, 1244, 1382, 1402, 1778, 1798
AluI AGCT 9 cut(s) 87, 193, 857, 914, 1244, 1382, 1402, 1778, 1798
Alw21I GWGCWC 4 cut(s) 435, 558, 1327, 1723
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 6 cut(s) 75, 267, 380, 480, 911, 1040
Ama87I CYCGRG 5 cut(s) 203, 379, 610, 1433, 1829
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 8 cut(s) 30, 51, 134, 171, 342, 741, 758, 1257
ApeKI GCWGC 2 cut(s) 764, 845
AsiGI ACCGGT 3 cut(s) 965, 1491, 1887
AspLEI GCGC 7 cut(s) 28, 64, 283, 421, 469, 1398, 1794
AspS9I GGNCC 3 cut(s) 31, 758, 1928
AsuHPI GGTGA 5 cut(s) 87, 268, 289, 850, 955
AvaI CYCGRG 5 cut(s) 203, 379, 610, 1433, 1829
AvaII GGWCC 1 cut(s) 1928
AxyI CCTNAGG 1 cut(s) 218
BaeGI GKGCMC 2 cut(s) 507, 573
BalI TGGCCA 1 cut(s) 743
BanI GGYRCC 2 cut(s) 1395, 1791
BbsI GAAGAC 2 cut(s) 356, 876
Bbv12I GWGCWC 4 cut(s) 435, 558, 1327, 1723
BbvI GCAGC 2 cut(s) 751, 832
BccI CCATC 3 cut(s) 714, 1358, 1754
BceAI ACGGC 5 cut(s) 59, 86, 427, 432, 480
BcgI CGANNNNNNTGC 4 cut(s) 1424, 1458, 1820, 1854
BciT130I CCWGG 6 cut(s) 133, 170, 341, 428, 575, 1039
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 5 cut(s) 525, 1446, 1595, 1842, 1990
BfmI CTRYAG 2 cut(s) 1377, 1773
BfoI RGCGCY 5 cut(s) 29, 65, 470, 1399, 1795
BfuAI ACCTGC 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 4 cut(s) 37, 345, 765, 846
BlsI GCNGC 4 cut(s) 38, 346, 766, 847
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 6 cut(s) 133, 170, 341, 428, 575, 1039
Bme18I GGWCC 1 cut(s) 1928
BmeRI GACNNNNNGTC 2 cut(s) 382, 930
BmeT110I CYCGRG 5 cut(s) 203, 379, 610, 1433, 1829
BmgBI CACGTC 2 cut(s) 568, 649
BmgT120I GGNCC 3 cut(s) 31, 758, 1928
BmiI GGNNCC 4 cut(s) 224, 1397, 1793, 1929
BmrFI CCNGG 6 cut(s) 133, 170, 341, 428, 575, 1039
BmsI GCATC 7 cut(s) 116, 802, 1043, 1444, 1453, 1840, 1849
BoxI GACNNNNGTC 1 cut(s) 293
BpiI GAAGAC 2 cut(s) 356, 876
BpmI CTGGAG 3 cut(s) 179, 449, 497
BpuEI CTTGAG 1 cut(s) 212
Bsa29I ATCGAT 1 cut(s) 1645
BsaHI GRCGYC 3 cut(s) 180, 1396, 1792
BsaJI CCNNGG 6 cut(s) 131, 153, 174, 549, 573, 1923
BsaWI WCCGGW 4 cut(s) 627, 965, 1491, 1887
BsaXI ACNNNNNCTCC 2 cut(s) 1087, 1117
Bsc4I CCNNNNNNNGG 6 cut(s) 39, 43, 175, 219, 1479, 1875
Bse118I RCCGGY 5 cut(s) 39, 58, 965, 1491, 1887
Bse1I ACTGG 6 cut(s) 727, 1210, 1566, 1587, 1961, 1982
Bse21I CCTNAGG 1 cut(s) 218
Bse3DI GCAATG 3 cut(s) 1086, 1427, 1823
BseBI CCWGG 6 cut(s) 133, 170, 341, 428, 575, 1039
BseCI ATCGAT 1 cut(s) 1645
BseDI CCNNGG 6 cut(s) 131, 153, 174, 549, 573, 1923
BseGI GGATG 6 cut(s) 107, 270, 1034, 1255, 1369, 1765
BseLI CCNNNNNNNGG 6 cut(s) 39, 43, 175, 219, 1479, 1875
BseMI GCAATG 3 cut(s) 1086, 1427, 1823
BseMII CTCAG 3 cut(s) 209, 963, 1122
BseNI ACTGG 6 cut(s) 727, 1210, 1566, 1587, 1961, 1982
BseRI GAGGAG 2 cut(s) 129, 683
BseSI GKGCMC 2 cut(s) 507, 573
BseXI GCAGC 2 cut(s) 751, 832
Bsh1236I CGCG 5 cut(s) 281, 294, 326, 347, 356
Bsh1285I CGRYCG 4 cut(s) 44, 150, 166, 360
BshFI GGCC 8 cut(s) 32, 53, 136, 173, 344, 743, 760, 1259
BshNI GGYRCC 2 cut(s) 1395, 1791
BshTI ACCGGT 3 cut(s) 965, 1491, 1887
BshVI ATCGAT 1 cut(s) 1645
BsiEI CGRYCG 4 cut(s) 44, 150, 166, 360
BsiHKAI GWGCWC 4 cut(s) 435, 558, 1327, 1723
BsiHKCI CYCGRG 5 cut(s) 203, 379, 610, 1433, 1829
BsiSI CCGG 8 cut(s) 40, 59, 284, 628, 966, 1238, 1492, 1888
BslFI GGGAC 3 cut(s) 463, 1381, 1777
BslI CCNNNNNNNGG 6 cut(s) 39, 43, 175, 219, 1479, 1875
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 3 cut(s) 463, 1381, 1777
BsmI GAATGC 3 cut(s) 517, 722, 1022
BsnI GGCC 8 cut(s) 32, 53, 136, 173, 344, 743, 760, 1259
BsoBI CYCGRG 5 cut(s) 203, 379, 610, 1433, 1829
Bsp1286I GDGCHC 6 cut(s) 435, 507, 558, 573, 1327, 1723
Bsp19I CCATGG 2 cut(s) 153, 1923
Bsp68I TCGCGA 1 cut(s) 326
BspACI CCGC 9 cut(s) 34, 37, 99, 128, 143, 150, 279, 345, 354
BspANI GGCC 8 cut(s) 32, 53, 136, 173, 344, 743, 760, 1259
BspCNI CTCAG 3 cut(s) 210, 964, 1123
BspDI ATCGAT 1 cut(s) 1645
BspFNI CGCG 5 cut(s) 281, 294, 326, 347, 356
BspHI TCATGA 1 cut(s) 1639
BspLI GGNNCC 4 cut(s) 224, 1397, 1793, 1929
BspMI ACCTGC 1 cut(s) 24
BspPI GGATC 6 cut(s) 75, 267, 380, 480, 911, 1040
BspT107I GGYRCC 2 cut(s) 1395, 1791
BsrDI GCAATG 3 cut(s) 1086, 1427, 1823
BsrFI RCCGGY 5 cut(s) 39, 58, 965, 1491, 1887
BsrI ACTGG 6 cut(s) 727, 1210, 1566, 1587, 1961, 1982
BssAI RCCGGY 5 cut(s) 39, 58, 965, 1491, 1887
BssECI CCNNGG 6 cut(s) 131, 153, 174, 549, 573, 1923
BssNI GRCGYC 3 cut(s) 180, 1396, 1792
BssT1I CCWWGG 3 cut(s) 153, 549, 1923
Bst2UI CCWGG 6 cut(s) 133, 170, 341, 428, 575, 1039
Bst4CI ACNGT 6 cut(s) 301, 314, 361, 1474, 1534, 1870
Bst6I CTCTTC 1 cut(s) 452
BstACI GRCGYC 3 cut(s) 180, 1396, 1792
BstAPI GCANNNNNTGC 2 cut(s) 1013, 1919
BstDEI CTNAG 3 cut(s) 218, 972, 1131
BstDSI CCRYGG 2 cut(s) 153, 1923
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 6 cut(s) 107, 270, 1034, 1255, 1369, 1765
BstFNI CGCG 5 cut(s) 281, 294, 326, 347, 356
BstH2I RGCGCY 5 cut(s) 29, 65, 470, 1399, 1795
BstHHI GCGC 7 cut(s) 28, 64, 283, 421, 469, 1398, 1794
BstMAI GTCTC 1 cut(s) 281
BstMCI CGRYCG 4 cut(s) 44, 150, 166, 360
BstNI CCWGG 6 cut(s) 133, 170, 341, 428, 575, 1039
BstNSI RCATGY 1 cut(s) 716
BstPAI GACNNNNGTC 1 cut(s) 293
BstSCI CCNGG 6 cut(s) 131, 168, 339, 426, 573, 1037
BstSFI CTRYAG 2 cut(s) 1377, 1773
BstSLI GKGCMC 2 cut(s) 507, 573
BstUI CGCG 5 cut(s) 281, 294, 326, 347, 356
BstV1I GCAGC 2 cut(s) 751, 832
BstV2I GAAGAC 2 cut(s) 356, 876
BstX2I RGATCY 2 cut(s) 472, 1045
BstYI RGATCY 2 cut(s) 472, 1045
Bsu15I ATCGAT 1 cut(s) 1645
Bsu36I CCTNAGG 1 cut(s) 218
BsuRI GGCC 8 cut(s) 32, 53, 136, 173, 344, 743, 760, 1259
BsuTUI ATCGAT 1 cut(s) 1645
BtgI CCRYGG 2 cut(s) 153, 1923
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 2 cut(s) 568, 649
BtsCI GGATG 6 cut(s) 107, 270, 1034, 1255, 1369, 1765
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 3 cut(s) 822, 1033, 1203
BtuMI TCGCGA 1 cut(s) 326
BveI ACCTGC 1 cut(s) 24
CciI TCATGA 1 cut(s) 1639
CfoI GCGC 7 cut(s) 28, 64, 283, 421, 469, 1398, 1794
Cfr10I RCCGGY 5 cut(s) 39, 58, 965, 1491, 1887
Cfr13I GGNCC 3 cut(s) 31, 758, 1928
ClaI ATCGAT 1 cut(s) 1645
CseI GACGC 4 cut(s) 283, 1147, 1579, 1974
Csp6I GTAC 2 cut(s) 232, 730
CspAI ACCGGT 3 cut(s) 965, 1491, 1887
CviQI GTAC 2 cut(s) 232, 730
DdeI CTNAG 3 cut(s) 218, 972, 1131
DinI GGCGCC 2 cut(s) 1397, 1793
DriI GACNNNNNGTC 2 cut(s) 382, 930
EaeI YGGCCR 3 cut(s) 171, 342, 741
Eam1104I CTCTTC 1 cut(s) 452
Eam1105I GACNNNNNGTC 2 cut(s) 382, 930
EarI CTCTTC 1 cut(s) 452
EciI GGCGGA 1 cut(s) 132
Eco130I CCWWGG 3 cut(s) 153, 549, 1923
Eco147I AGGCCT 2 cut(s) 53, 136
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 1 cut(s) 1928
Eco47III AGCGCT 1 cut(s) 27
Eco57I CTGAAG 4 cut(s) 782, 834, 1404, 1800
Eco81I CCTNAGG 1 cut(s) 218
Eco88I CYCGRG 5 cut(s) 203, 379, 610, 1433, 1829
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 1928
EcoRII CCWGG 6 cut(s) 131, 168, 339, 426, 573, 1037
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 3 cut(s) 153, 549, 1923
EgeI GGCGCC 2 cut(s) 1397, 1793
EheI GGCGCC 2 cut(s) 1397, 1793
ErhI CCWWGG 3 cut(s) 153, 549, 1923
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 3 cut(s) 463, 1381, 1777
FauI CCCGC 2 cut(s) 41, 106
FauNDI CATATG 2 cut(s) 1411, 1807
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 4 cut(s) 37, 345, 765, 846
FokI GGATG 6 cut(s) 94, 257, 1021, 1262, 1376, 1772
Fsp4HI GCNGC 4 cut(s) 37, 345, 765, 846
FspBI CTAG 5 cut(s) 525, 1446, 1595, 1842, 1990
GlaI GCGC 7 cut(s) 27, 63, 282, 420, 468, 1397, 1793
GluI GCNGC 4 cut(s) 37, 345, 765, 846
GsuI CTGGAG 3 cut(s) 179, 449, 497
HaeII RGCGCY 5 cut(s) 29, 65, 470, 1399, 1795
HaeIII GGCC 8 cut(s) 32, 53, 136, 173, 344, 743, 760, 1259
HapII CCGG 8 cut(s) 40, 59, 284, 628, 966, 1238, 1492, 1888
HgaI GACGC 4 cut(s) 283, 1147, 1579, 1974
HhaI GCGC 7 cut(s) 28, 64, 283, 421, 469, 1398, 1794
Hin1I GRCGYC 3 cut(s) 180, 1396, 1792
Hin6I GCGC 7 cut(s) 26, 62, 281, 419, 467, 1396, 1792
HinP1I GCGC 7 cut(s) 26, 62, 281, 419, 467, 1396, 1792
HincII GTYRAC 1 cut(s) 1137
HindII GTYRAC 1 cut(s) 1137
HindIII AAGCTT 1 cut(s) 1242
HinfI GANTC 6 cut(s) 321, 332, 377, 1052, 1422, 1818
HpaII CCGG 8 cut(s) 40, 59, 284, 628, 966, 1238, 1492, 1888
HphI GGTGA 5 cut(s) 87, 268, 289, 850, 955
Hpy166II GTNNAC 3 cut(s) 160, 900, 1137
Hpy188III TCNNGA 9 cut(s) 196, 205, 325, 370, 394, 476, 852, 1049, 1640
Hpy8I GTNNAC 3 cut(s) 160, 900, 1137
Hpy99I CGWCG 3 cut(s) 107, 331, 365
HpyAV CCTTC 3 cut(s) 963, 1361, 1757
HpyCH4III ACNGT 6 cut(s) 301, 314, 361, 1474, 1534, 1870
HpyCH4IV ACGT 3 cut(s) 180, 567, 648
HpyF3I CTNAG 3 cut(s) 218, 972, 1131
HpySE526I ACGT 3 cut(s) 180, 567, 648
Hsp92I GRCGYC 3 cut(s) 180, 1396, 1792
HspAI GCGC 7 cut(s) 26, 62, 281, 419, 467, 1396, 1792
KasI GGCGCC 2 cut(s) 1395, 1791
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
LmnI GCTCC 7 cut(s) 92, 198, 430, 529, 832, 854, 1031
Lsp1109I GCAGC 2 cut(s) 751, 832
LweI GCATC 7 cut(s) 116, 802, 1043, 1444, 1453, 1840, 1849
MaeI CTAG 5 cut(s) 525, 1446, 1595, 1842, 1990
MaeII ACGT 3 cut(s) 180, 567, 648
MaeIII GTNAC 2 cut(s) 295, 644
MfeI CAATTG 1 cut(s) 1914
MflI RGATCY 2 cut(s) 472, 1045
MhlI GDGCHC 6 cut(s) 435, 507, 558, 573, 1327, 1723
MlsI TGGCCA 1 cut(s) 743
MluCI AATT 8 cut(s) 802, 981, 1449, 1545, 1615, 1845, 1914, 1940
MluI ACGCGT 1 cut(s) 292
MluNI TGGCCA 1 cut(s) 743
Mly113I GGCGCC 2 cut(s) 1396, 1792
MlyI GAGTC 4 cut(s) 330, 341, 371, 1046
MmeI TCCRAC 1 cut(s) 944
Mox20I TGGCCA 1 cut(s) 743
MreI CGCCGGCG 1 cut(s) 58
MroNI GCCGGC 1 cut(s) 58
MscI TGGCCA 1 cut(s) 743
MseI TTAA 8 cut(s) 774, 810, 861, 888, 1155, 1337, 1614, 1733
MslI CAYNNNNRTG 5 cut(s) 510, 711, 717, 1362, 1758
Msp20I TGGCCA 1 cut(s) 743
MspA1I CMGCKG 1 cut(s) 914
MspI CCGG 8 cut(s) 40, 59, 284, 628, 966, 1238, 1492, 1888
MspR9I CCNGG 6 cut(s) 133, 170, 341, 428, 575, 1039
MunI CAATTG 1 cut(s) 1914
Mva1269I GAATGC 3 cut(s) 517, 722, 1022
MvaI CCWGG 6 cut(s) 133, 170, 341, 428, 575, 1039
MvnI CGCG 5 cut(s) 281, 294, 326, 347, 356
NaeI GCCGGC 1 cut(s) 60
NarI GGCGCC 2 cut(s) 1396, 1792
NcoI CCATGG 2 cut(s) 153, 1923
NdeI CATATG 2 cut(s) 1411, 1807
NgoMIV GCCGGC 1 cut(s) 58
NlaIV GGNNCC 4 cut(s) 224, 1397, 1793, 1929
NmeAIII GCCGAG 2 cut(s) 199, 662
NmuCI GTSAC 2 cut(s) 295, 644
NruI TCGCGA 1 cut(s) 326
NspI RCATGY 1 cut(s) 716
PaeR7I CTCGAG 2 cut(s) 203, 610
PagI TCATGA 1 cut(s) 1639
PaqCI CACCTGC 1 cut(s) 24
PceI AGGCCT 2 cut(s) 53, 136
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 245, 564
PctI GAATGC 3 cut(s) 517, 722, 1022
PdiI GCCGGC 1 cut(s) 60
PfeI GAWTC 2 cut(s) 1422, 1818
PflFI GACNNNGTC 1 cut(s) 331
PfoI TCCNGGA 2 cut(s) 426, 1037
PinAI ACCGGT 3 cut(s) 965, 1491, 1887
PkrI GCNGC 4 cut(s) 38, 346, 766, 847
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 4 cut(s) 329, 340, 371, 1046
PluTI GGCGCC 2 cut(s) 1399, 1795
PpsI GAGTC 4 cut(s) 329, 340, 371, 1046
PpuMI RGGWCCY 1 cut(s) 1928
PshAI GACNNNNGTC 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 1928
Psp6I CCWGG 6 cut(s) 131, 168, 339, 426, 573, 1037
PspGI CCWGG 6 cut(s) 131, 168, 339, 426, 573, 1037
PspN4I GGNNCC 4 cut(s) 224, 1397, 1793, 1929
PspPI GGNCC 3 cut(s) 31, 758, 1928
PspPPI RGGWCCY 1 cut(s) 1928
PspXI VCTCGAGB 1 cut(s) 610
PsuI RGATCY 2 cut(s) 472, 1045
PsyI GACNNNGTC 1 cut(s) 331
PvuI CGATCG 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 914
RruI TCGCGA 1 cut(s) 326
RsaI GTAC 2 cut(s) 233, 731
RsaNI GTAC 2 cut(s) 232, 730
RseI CAYNNNNRTG 5 cut(s) 510, 711, 717, 1362, 1758
SaqAI TTAA 8 cut(s) 774, 810, 861, 888, 1155, 1337, 1614, 1733
SatI GCNGC 4 cut(s) 37, 345, 765, 846
Sau96I GGNCC 3 cut(s) 31, 758, 1928
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 4 cut(s) 330, 341, 371, 1046
ScrFI CCNGG 6 cut(s) 133, 170, 341, 428, 575, 1039
SduI GDGCHC 6 cut(s) 435, 507, 558, 573, 1327, 1723
SfaNI GCATC 7 cut(s) 116, 802, 1043, 1444, 1453, 1840, 1849
SfcI CTRYAG 2 cut(s) 1377, 1773
SfoI GGCGCC 2 cut(s) 1397, 1793
Sfr274I CTCGAG 2 cut(s) 203, 610
SgrAI CRCCGGYG 1 cut(s) 58
SinI GGWCC 1 cut(s) 1928
SlaI CTCGAG 2 cut(s) 203, 610
SmiMI CAYNNNNRTG 5 cut(s) 510, 711, 717, 1362, 1758
SmlI CTYRAG 3 cut(s) 203, 227, 610
SmoI CTYRAG 3 cut(s) 203, 227, 610
Sse9I AATT 8 cut(s) 802, 981, 1449, 1545, 1615, 1845, 1914, 1940
SseBI AGGCCT 2 cut(s) 53, 136
SsiI CCGC 9 cut(s) 34, 37, 99, 128, 143, 150, 279, 345, 354
SspDI GGCGCC 2 cut(s) 1395, 1791
SspI AATATT 1 cut(s) 1662
SspMI CTAG 5 cut(s) 525, 1446, 1595, 1842, 1990
StuI AGGCCT 2 cut(s) 53, 136
StyD4I CCNGG 6 cut(s) 131, 168, 339, 426, 573, 1037
StyI CCWWGG 3 cut(s) 153, 549, 1923
TaaI ACNGT 6 cut(s) 301, 314, 361, 1474, 1534, 1870
TaiI ACGT 3 cut(s) 183, 570, 651
TaqII GACCGA 1 cut(s) 34
TasI AATT 8 cut(s) 802, 981, 1449, 1545, 1615, 1845, 1914, 1940
TatI WGTACW 1 cut(s) 729
TauI GCSGC 2 cut(s) 39, 347
TfiI GAWTC 2 cut(s) 1422, 1818
Tru1I TTAA 8 cut(s) 774, 810, 861, 888, 1155, 1337, 1614, 1733
Tru9I TTAA 8 cut(s) 774, 810, 861, 888, 1155, 1337, 1614, 1733
TscAI CASTG 3 cut(s) 829, 1033, 1210
TseFI GTSAC 2 cut(s) 295, 644
TseI GCWGC 2 cut(s) 764, 845
Tsp45I GTSAC 2 cut(s) 295, 644
TspDTI ATGAA 7 cut(s) 23, 705, 882, 945, 1080, 1612, 1628
TspGWI ACGGA 3 cut(s) 272, 344, 467
TspRI CASTG 3 cut(s) 829, 1033, 1210
Tth111I GACNNNGTC 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 1928
XagI CCTNNNNNAGG 1 cut(s) 173
XceI RCATGY 1 cut(s) 716
XhoI CTCGAG 2 cut(s) 203, 610
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 5 cut(s) 525, 1446, 1595, 1842, 1990
ZraI GACGTC 1 cut(s) 181
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.