MD17G1057400.v1.1

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015224
Physical Location & Seq
Forward (+)
4687280 .. 4691247
3968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1057400.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGCGATGAAGCACCTTCTTACTACCCTAGCTCGCCGCCGGCCCCACAGACCGCCGGCTTCTCTCACCGCAGCCGTCCGATCCCAATCGACCTCGCCCGCCGTCGCTACGTCACCTTCATCCCCATCCCTCCCCTCCCCTCCTCCACCAACCGCCATGATCTACGATCGATTGGCCGAGGGCGTCAAATCGAAGATCAAAAGGCTCGAGAACCCGGACCCGAGGTTCCTTAAGTACGGGTCACCCCACCCGAGTCTCACGGACCACAGCCACATCCTCACCGCACCCGAGACACGTGTCACCACACTCCCCAACGGCCTTCGGGTGGCCACCGAGTCGAACCTCGCGTCTAAGACCGCCACTGTCGGTGTCTGGATCGACGCCGGGTCAAGGTTCGAGGACGACGAGACCAACGGCACTGCGCATTTCCTGGAGCACATGATCTTCAAGGGGACGGAGCAGAGGACGGCCCGTGATCTGGAGGAGGAGGTCGAGAACATGGGAGGTCACTTGAATGCGTACACATCGAGGGAGCAGACCACGTACTATGCCAAGGTGCTCGATTCCGATGTGCCGAAGGCACTTTCGATTTTGGCTGATATCTTGCAGAACTCCAAGTTCGATGAGGACAGGATTTTGCGCGAGCGCGATGTGATTTTGAGGGAAATGGAGGAGGTCGAGAAACAACCCGAAGAAGTCATTTTTGACCATCTGCATGCAACTGCATTCCAGTACTCTCCTCTAGCCAGAACTATTCTTGGACCTGCTGACAACATTAAGACCATCTCCAAAGAGCATCTTCAGAACTATATCAAAACACATTACACTGCTCCCAGAATGGTTATTGCTGCTTCAGGAGCTATCAAGCATGAAGATCTTGTTGGGTCAGTTAAGGGTTTGTTCACAAAACTCTCAGGAGATCCTACCACCGCGGGTCAGTTGGTTGCGAAAGAACCTTCATTTTTTACTGGTTCTGAGGTCAGAATTGTGGATGATGACTTGCCTTTGGCACATTTTGCGGTTGCGTTCAGTGGTGCATCTTGGACTGATCCCGACTCTATTCCTCTAATGGTCATGCAAGCGATGCTGGGTTCATGGAATAAAAATGCTGGTGGTGGAAAGCACATGGGTTCAGAGCTGGCACAGAGGGTTGCCATTAATGACATAGCAGAAAGCTATATGTCTTTCAACACCAACTATAAGGATACTGGCTTATTTGGTGTTTTTGCTACTGCTCCGGCGGATTGCTTAGATGATTTGGCCTATGCAATCATGTATGAGGTCACCAAGTTAGTTTATCGAGTTTCAGAAGCTGATGTTATTCGTGCTCGTAATCAGTTGAAATCTTCGCTGCTTCTGCATTTGGATGGGACAAGTGCTGTAGCTGAAGATATTGGGCGCCAGTTACTTACATATGGTCGGAGAATCCCCTTGCCCGAGCTGTTTGCTAGAATCGATTCTGTTGATGCAAGCACCATCAAACGTGTTGCCAACCGATTCATCTATGACAGGGATATCGCAATTGCAGCCATGGGTCCGGTTACAAAATTGCCTGACTACAACTGGTTCAGACGCCGCACCTACTGGAACAGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

533

Amino Acids

59.09

Weight (kDa)

6.37

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 108 - 255 1.5e-55 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 262 - 447 9.8e-36 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 423
Acc36I ACCTGC 1 cut(s) 772
AccB1I GGYRCC 1 cut(s) 1398
AccII CGCG 4 cut(s) 347, 642, 648, 932
AclWI GGATC 4 cut(s) 75, 383, 914, 1043
AcoI YGGCCR 2 cut(s) 174, 327
AcuI CTGAAG 3 cut(s) 785, 837, 1407
AcvI CACGTG 1 cut(s) 296
AcyI GRCGYC 4 cut(s) 183, 381, 1399, 1573
AfaI GTAC 4 cut(s) 236, 521, 545, 734
AfiI CCNNNNNNNGG 3 cut(s) 325, 390, 478
AflII CTTAAG 1 cut(s) 230
AflIII ACRYGT 3 cut(s) 293, 295, 1483
AgsI TTSAA 4 cut(s) 448, 514, 1189, 1342
AhdI GACNNNNNGTC 1 cut(s) 385
AjnI CCWGG 1 cut(s) 429
AjuI GAANNNNNNNTTGG 2 cut(s) 864, 896
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
AluBI AGCT 7 cut(s) 32, 860, 1138, 1176, 1313, 1385, 1441
AluI AGCT 7 cut(s) 32, 860, 1138, 1176, 1313, 1385, 1441
Alw21I GWGCWC 3 cut(s) 438, 561, 1330
Alw26I GTCTC 3 cut(s) 260, 284, 401
AlwI GGATC 4 cut(s) 75, 383, 914, 1043
AlwNI CAGNNNCTG 1 cut(s) 1313
Ama87I CYCGRG 5 cut(s) 206, 220, 250, 287, 1436
AoxI GGCC 6 cut(s) 41, 174, 316, 327, 468, 1260
ApeKI GCWGC 4 cut(s) 71, 848, 1351, 1526
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AseI ATTAAT 1 cut(s) 1158
Asp700I GAANNNNTTC 1 cut(s) 1456
AspLEI GCGC 4 cut(s) 424, 642, 648, 1401
AspS9I GGNCC 6 cut(s) 42, 217, 262, 469, 761, 1535
AsuC2I CCSGG 2 cut(s) 215, 385
AsuHPI GGTGA 6 cut(s) 58, 105, 234, 271, 292, 1276
AvaI CYCGRG 5 cut(s) 206, 220, 250, 287, 1436
AvaII GGWCC 4 cut(s) 217, 262, 761, 1535
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 1 cut(s) 1398
BbrPI CACGTG 1 cut(s) 296
Bbv12I GWGCWC 3 cut(s) 438, 561, 1330
BbvI GCAGC 4 cut(s) 83, 835, 1338, 1538
BccI CCATC 5 cut(s) 133, 717, 791, 1361, 1484
BceAI ACGGC 5 cut(s) 59, 86, 331, 430, 483
BcgI CGANNNNNNTGC 2 cut(s) 1427, 1461
BciT130I CCWGG 1 cut(s) 431
BciVI GTATCC 1 cut(s) 1198
BcnI CCSGG 2 cut(s) 215, 385
BcoDI GTCTC 3 cut(s) 260, 284, 401
BfaI CTAG 3 cut(s) 29, 743, 1449
BfmI CTRYAG 1 cut(s) 1380
BfoI RGCGCY 1 cut(s) 1402
BfrI CTTAAG 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 772
BfuI GTATCC 1 cut(s) 1198
BglII AGATCT 1 cut(s) 874
BisI GCNGC 6 cut(s) 37, 72, 849, 1352, 1527, 1576
BlsI GCNGC 6 cut(s) 38, 73, 850, 1353, 1528, 1577
BmcAI AGTACT 1 cut(s) 734
Bme1390I CCNGG 3 cut(s) 215, 385, 431
Bme18I GGWCC 4 cut(s) 217, 262, 761, 1535
BmeRI GACNNNNNGTC 1 cut(s) 385
BmeT110I CYCGRG 5 cut(s) 206, 220, 250, 287, 1436
BmgT120I GGNCC 6 cut(s) 42, 217, 262, 469, 761, 1535
BmiI GGNNCC 5 cut(s) 44, 219, 227, 1400, 1536
BmrFI CCNGG 3 cut(s) 215, 385, 431
BmsI GCATC 4 cut(s) 805, 1046, 1074, 1456
BoxI GACNNNNGTC 1 cut(s) 296
BpmI CTGGAG 2 cut(s) 452, 500
BpuMI CCSGG 2 cut(s) 215, 385
Bsa29I ATCGAT 2 cut(s) 169, 1455
BsaAI YACGTR 2 cut(s) 296, 543
BsaBI GATNNNNATC 1 cut(s) 85
BsaHI GRCGYC 4 cut(s) 183, 381, 1399, 1573
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 5 cut(s) 177, 221, 552, 930, 1530
BsaWI WCCGGW 1 cut(s) 1537
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 390, 478
Bse118I RCCGGY 2 cut(s) 39, 55
Bse1I ACTGG 6 cut(s) 730, 973, 1213, 1402, 1568, 1589
Bse8I GATNNNNATC 1 cut(s) 85
BseBI CCWGG 1 cut(s) 431
BseCI ATCGAT 2 cut(s) 169, 1455
BseDI CCNNGG 5 cut(s) 177, 221, 552, 930, 1530
BseGI GGATG 5 cut(s) 119, 125, 273, 997, 1372
BseJI GATNNNNATC 1 cut(s) 85
BseLI CCNNNNNNNGG 3 cut(s) 325, 390, 478
BseMII CTCAG 2 cut(s) 927, 966
BseNI ACTGG 6 cut(s) 730, 973, 1213, 1402, 1568, 1589
BseRI GAGGAG 5 cut(s) 132, 497, 500, 686, 729
BseXI GCAGC 4 cut(s) 83, 835, 1338, 1538
BseYI CCCAGC 1 cut(s) 1087
Bsh1236I CGCG 4 cut(s) 347, 642, 648, 932
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 6 cut(s) 43, 176, 318, 329, 470, 1262
BshNI GGYRCC 1 cut(s) 1398
BshVI ATCGAT 2 cut(s) 169, 1455
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 3 cut(s) 438, 561, 1330
BsiHKCI CYCGRG 5 cut(s) 206, 220, 250, 287, 1436
BsiSI CCGG 6 cut(s) 40, 56, 215, 384, 1238, 1538
BslFI GGGAC 2 cut(s) 466, 1384
BslI CCNNNNNNNGG 3 cut(s) 325, 390, 478
BsmAI GTCTC 3 cut(s) 260, 284, 401
BsmFI GGGAC 2 cut(s) 466, 1384
BsmI GAATGC 2 cut(s) 520, 725
BsnI GGCC 6 cut(s) 43, 176, 318, 329, 470, 1262
Bso31I GGTCTC 1 cut(s) 401
BsoBI CYCGRG 5 cut(s) 206, 220, 250, 287, 1436
Bsp1286I GDGCHC 3 cut(s) 438, 561, 1330
Bsp19I CCATGG 1 cut(s) 1530
BspANI GGCC 6 cut(s) 43, 176, 318, 329, 470, 1262
BspCNI CTCAG 2 cut(s) 926, 967
BspDI ATCGAT 2 cut(s) 169, 1455
BspFNI CGCG 4 cut(s) 347, 642, 648, 932
BspLI GGNNCC 5 cut(s) 44, 219, 227, 1400, 1536
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 4 cut(s) 75, 383, 914, 1043
BspT107I GGYRCC 1 cut(s) 1398
BspTI CTTAAG 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 401
BsrFI RCCGGY 2 cut(s) 39, 55
BsrI ACTGG 6 cut(s) 730, 973, 1213, 1402, 1568, 1589
BssAI RCCGGY 2 cut(s) 39, 55
BssECI CCNNGG 5 cut(s) 177, 221, 552, 930, 1530
BssNI GRCGYC 4 cut(s) 183, 381, 1399, 1573
BssT1I CCWWGG 2 cut(s) 552, 1530
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 1 cut(s) 364
BstACI GRCGYC 4 cut(s) 183, 381, 1399, 1573
BstAFI CTTAAG 1 cut(s) 230
BstAPI GCANNNNNTGC 2 cut(s) 1016, 1084
BstBAI YACGTR 2 cut(s) 296, 543
BstC8I GCNNGC 9 cut(s) 34, 41, 57, 99, 644, 717, 1080, 1140, 1471
BstDEI CTNAG 4 cut(s) 351, 913, 975, 1249
BstDSI CCRYGG 2 cut(s) 930, 1530
BstEII GGTNACC 2 cut(s) 240, 1282
BstF5I GGATG 5 cut(s) 119, 125, 273, 997, 1372
BstFNI CGCG 4 cut(s) 347, 642, 648, 932
BstH2I RGCGCY 1 cut(s) 1402
BstHHI GCGC 4 cut(s) 424, 642, 648, 1401
BstMAI GTCTC 3 cut(s) 260, 284, 401
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 5 cut(s) 857, 1016, 1084, 1357, 1526
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 1 cut(s) 719
BstPAI GACNNNNGTC 1 cut(s) 296
BstPI GGTNACC 2 cut(s) 240, 1282
BstSCI CCNGG 3 cut(s) 213, 383, 429
BstSFI CTRYAG 1 cut(s) 1380
BstUI CGCG 4 cut(s) 347, 642, 648, 932
BstV1I GCAGC 4 cut(s) 83, 835, 1338, 1538
BstX2I RGATCY 2 cut(s) 874, 919
BstYI RGATCY 2 cut(s) 874, 919
Bsu15I ATCGAT 2 cut(s) 169, 1455
BsuI GTATCC 1 cut(s) 1198
BsuRI GGCC 6 cut(s) 43, 176, 318, 329, 470, 1262
BsuTUI ATCGAT 2 cut(s) 169, 1455
BtgI CCRYGG 2 cut(s) 930, 1530
BtgZI GCGATG 3 cut(s) 20, 663, 1097
BtsCI GGATG 5 cut(s) 119, 125, 273, 997, 1372
BtsI GCAGTG 2 cut(s) 417, 825
BtsIMutI CAGTG 4 cut(s) 360, 417, 825, 1036
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 9 cut(s) 34, 41, 57, 99, 644, 717, 1080, 1140, 1471
CaiI CAGNNNCTG 1 cut(s) 1313
CfoI GCGC 4 cut(s) 424, 642, 648, 1401
Cfr10I RCCGGY 2 cut(s) 39, 55
Cfr13I GGNCC 6 cut(s) 42, 217, 262, 469, 761, 1535
Cfr42I CCGCGG 1 cut(s) 933
ClaI ATCGAT 2 cut(s) 169, 1455
CseI GACGC 4 cut(s) 172, 336, 389, 1581
Csp6I GTAC 4 cut(s) 235, 520, 544, 733
CviQI GTAC 4 cut(s) 235, 520, 544, 733
DdeI CTNAG 4 cut(s) 351, 913, 975, 1249
DinI GGCGCC 1 cut(s) 1400
DriI GACNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 2 cut(s) 174, 327
Eam1105I GACNNNNNGTC 1 cut(s) 385
EciI GGCGGA 1 cut(s) 1256
Eco130I CCWWGG 2 cut(s) 552, 1530
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 2 cut(s) 601, 1516
Eco47I GGWCC 4 cut(s) 217, 262, 761, 1535
Eco57I CTGAAG 3 cut(s) 785, 837, 1407
Eco72I CACGTG 1 cut(s) 296
Eco88I CYCGRG 5 cut(s) 206, 220, 250, 287, 1436
Eco91I GGTNACC 2 cut(s) 240, 1282
EcoO65I GGTNACC 2 cut(s) 240, 1282
EcoRII CCWGG 1 cut(s) 429
EcoRV GATATC 2 cut(s) 601, 1516
EcoT14I CCWWGG 2 cut(s) 552, 1530
EgeI GGCGCC 1 cut(s) 1400
EheI GGCGCC 1 cut(s) 1400
ErhI CCWWGG 2 cut(s) 552, 1530
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FaqI GGGAC 2 cut(s) 466, 1384
FauI CCCGC 2 cut(s) 106, 925
FauNDI CATATG 1 cut(s) 1414
Fnu4HI GCNGC 6 cut(s) 37, 72, 849, 1352, 1527, 1576
FokI GGATG 5 cut(s) 106, 112, 260, 1004, 1379
Fsp4HI GCNGC 6 cut(s) 37, 72, 849, 1352, 1527, 1576
FspBI CTAG 3 cut(s) 29, 743, 1449
FspI TGCGCA 1 cut(s) 423
GlaI GCGC 4 cut(s) 423, 641, 647, 1400
GluI GCNGC 6 cut(s) 37, 72, 849, 1352, 1527, 1576
GsaI CCCAGC 1 cut(s) 1091
GsuI CTGGAG 2 cut(s) 452, 500
HaeII RGCGCY 1 cut(s) 1402
HaeIII GGCC 6 cut(s) 43, 176, 318, 329, 470, 1262
HapII CCGG 6 cut(s) 40, 56, 215, 384, 1238, 1538
HgaI GACGC 4 cut(s) 172, 336, 389, 1581
HhaI GCGC 4 cut(s) 424, 642, 648, 1401
Hin1I GRCGYC 4 cut(s) 183, 381, 1399, 1573
Hin6I GCGC 4 cut(s) 422, 640, 646, 1399
HinP1I GCGC 4 cut(s) 422, 640, 646, 1399
HinfI GANTC 8 cut(s) 253, 335, 563, 1055, 1425, 1452, 1457, 1497
HpaII CCGG 6 cut(s) 40, 56, 215, 384, 1238, 1538
HphI GGTGA 6 cut(s) 58, 105, 234, 271, 292, 1276
Hpy166II GTNNAC 2 cut(s) 522, 903
Hpy188I TCNGA 9 cut(s) 80, 568, 804, 976, 983, 1135, 1309, 1422, 1571
Hpy188III TCNNGA 8 cut(s) 208, 373, 479, 493, 679, 855, 915, 1052
Hpy8I GTNNAC 2 cut(s) 522, 903
Hpy99I CGWCG 3 cut(s) 107, 383, 407
HpyAV CCTTC 5 cut(s) 26, 126, 329, 571, 966
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 4 cut(s) 110, 295, 542, 1483
HpyF10VI GCNNNNNNNGC 5 cut(s) 857, 1016, 1084, 1357, 1526
HpyF3I CTNAG 4 cut(s) 351, 913, 975, 1249
HpySE526I ACGT 4 cut(s) 110, 295, 542, 1483
Hsp92I GRCGYC 4 cut(s) 183, 381, 1399, 1573
HspAI GCGC 4 cut(s) 422, 640, 646, 1399
KasI GGCGCC 1 cut(s) 1398
KroI GCCGGC 2 cut(s) 39, 55
KroNI GCCGGC 2 cut(s) 41, 57
KspI CCGCGG 1 cut(s) 933
LmnI GCTCC 6 cut(s) 433, 457, 532, 835, 857, 1240
Lsp1109I GCAGC 4 cut(s) 83, 835, 1338, 1538
LweI GCATC 4 cut(s) 805, 1046, 1074, 1456
MaeI CTAG 3 cut(s) 29, 743, 1449
MaeII ACGT 4 cut(s) 110, 295, 542, 1483
MaeIII GTNAC 7 cut(s) 111, 240, 298, 506, 1282, 1404, 1540
MboII GAAGA 7 cut(s) 205, 436, 704, 791, 884, 1338, 1400
MfeI CAATTG 1 cut(s) 1521
MflI RGATCY 2 cut(s) 874, 919
MhlI GDGCHC 3 cut(s) 438, 561, 1330
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 3 cut(s) 984, 1521, 1547
MluNI TGGCCA 1 cut(s) 329
Mly113I GGCGCC 1 cut(s) 1399
MlyI GAGTC 3 cut(s) 262, 344, 1049
MmeI TCCRAC 1 cut(s) 1400
Mox20I TGGCCA 1 cut(s) 329
MroNI GCCGGC 2 cut(s) 39, 55
MroXI GAANNNNTTC 1 cut(s) 1456
MscI TGGCCA 1 cut(s) 329
MseI TTAA 4 cut(s) 231, 777, 891, 1158
MslI CAYNNNNRTG 3 cut(s) 513, 714, 1365
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 932
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 6 cut(s) 40, 56, 215, 384, 1238, 1538
MspR9I CCNGG 3 cut(s) 215, 385, 431
MunI CAATTG 1 cut(s) 1521
Mva1269I GAATGC 2 cut(s) 520, 725
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 4 cut(s) 347, 642, 648, 932
MwoI GCNNNNNNNGC 5 cut(s) 857, 1016, 1084, 1357, 1526
NaeI GCCGGC 2 cut(s) 41, 57
NarI GGCGCC 1 cut(s) 1399
NciI CCSGG 2 cut(s) 215, 385
NcoI CCATGG 1 cut(s) 1530
NdeI CATATG 1 cut(s) 1414
NgoMIV GCCGGC 2 cut(s) 39, 55
NlaIV GGNNCC 5 cut(s) 44, 219, 227, 1400, 1536
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 5 cut(s) 111, 240, 298, 506, 1282
NsbI TGCGCA 1 cut(s) 423
NspI RCATGY 1 cut(s) 719
PaeI GCATGC 1 cut(s) 719
PaeR7I CTCGAG 1 cut(s) 206
PcsI WCGNNNNNNNCGW 2 cut(s) 344, 402
PctI GAATGC 2 cut(s) 520, 725
PdiI GCCGGC 2 cut(s) 41, 57
PdmI GAANNNNTTC 1 cut(s) 1456
PfeI GAWTC 5 cut(s) 563, 1425, 1452, 1457, 1497
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 6 cut(s) 38, 73, 850, 1353, 1528, 1577
Ple19I CGATCG 1 cut(s) 169
PleI GAGTC 3 cut(s) 261, 343, 1049
PluTI GGCGCC 1 cut(s) 1402
PmaCI CACGTG 1 cut(s) 296
PmlI CACGTG 1 cut(s) 296
PpsI GAGTC 3 cut(s) 261, 343, 1049
Ppu21I YACGTR 2 cut(s) 296, 543
PshAI GACNNNNGTC 1 cut(s) 296
PshBI ATTAAT 1 cut(s) 1158
Psp6I CCWGG 1 cut(s) 429
PspCI CACGTG 1 cut(s) 296
PspEI GGTNACC 2 cut(s) 240, 1282
PspFI CCCAGC 1 cut(s) 1087
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 5 cut(s) 44, 219, 227, 1400, 1536
PspPI GGNCC 6 cut(s) 42, 217, 262, 469, 761, 1535
PstNI CAGNNNCTG 1 cut(s) 1313
PsuI RGATCY 2 cut(s) 874, 919
PvuI CGATCG 1 cut(s) 169
RsaI GTAC 4 cut(s) 236, 521, 545, 734
RsaNI GTAC 4 cut(s) 235, 520, 544, 733
RseI CAYNNNNRTG 3 cut(s) 513, 714, 1365
SacII CCGCGG 1 cut(s) 933
SaqAI TTAA 4 cut(s) 231, 777, 891, 1158
SatI GCNGC 6 cut(s) 37, 72, 849, 1352, 1527, 1576
Sau96I GGNCC 6 cut(s) 42, 217, 262, 469, 761, 1535
ScaI AGTACT 1 cut(s) 734
SchI GAGTC 3 cut(s) 262, 344, 1049
ScrFI CCNGG 3 cut(s) 215, 385, 431
SduI GDGCHC 3 cut(s) 438, 561, 1330
SfaNI GCATC 4 cut(s) 805, 1046, 1074, 1456
SfcI CTRYAG 1 cut(s) 1380
SfoI GGCGCC 1 cut(s) 1400
Sfr274I CTCGAG 1 cut(s) 206
Sfr303I CCGCGG 1 cut(s) 933
SgrBI CCGCGG 1 cut(s) 933
SinI GGWCC 4 cut(s) 217, 262, 761, 1535
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 3 cut(s) 513, 714, 1365
SmlI CTYRAG 2 cut(s) 206, 230
SmoI CTYRAG 2 cut(s) 206, 230
SphI GCATGC 1 cut(s) 719
Sse9I AATT 3 cut(s) 984, 1521, 1547
SspDI GGCGCC 1 cut(s) 1398
SspMI CTAG 3 cut(s) 29, 743, 1449
StyD4I CCNGG 3 cut(s) 213, 383, 429
StyI CCWWGG 2 cut(s) 552, 1530
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 4 cut(s) 113, 298, 545, 1486
TasI AATT 3 cut(s) 984, 1521, 1547
TatI WGTACW 1 cut(s) 732
TauI GCSGC 2 cut(s) 39, 1578
TfiI GAWTC 5 cut(s) 563, 1425, 1452, 1457, 1497
Tru1I TTAA 4 cut(s) 231, 777, 891, 1158
Tru9I TTAA 4 cut(s) 231, 777, 891, 1158
TscAI CASTG 4 cut(s) 367, 424, 832, 1036
TseFI GTSAC 5 cut(s) 111, 240, 298, 506, 1282
TseI GCWGC 4 cut(s) 71, 848, 1351, 1526
Tsp45I GTSAC 5 cut(s) 111, 240, 298, 506, 1282
TspDTI ATGAA 6 cut(s) 23, 108, 885, 948, 1083, 1489
TspGWI ACGGA 2 cut(s) 275, 470
TspRI CASTG 4 cut(s) 367, 424, 832, 1036
Vha464I CTTAAG 1 cut(s) 230
VpaK11BI GGWCC 4 cut(s) 217, 262, 761, 1535
VspI ATTAAT 1 cut(s) 1158
XceI RCATGY 1 cut(s) 719
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 1456
XspI CTAG 3 cut(s) 29, 743, 1449
ZrmI AGTACT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.