MD09G1064100.v1.1

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015224
Physical Location & Seq
Forward (+)
4393581 .. 4397779
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1064100.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGCGATGAAGCACCTTCTTACAACCCTAGCTCGCCGACGGCCCCACAGACCGCCGGCTGCTCTAACAGCAGCCGTTCGATCCCAGTCAACCTCACCCGCCGTCGCCACGTCATCTTCATCCCCATCCCTACCCTCCCCTCCTCCACCAACCGCCATGGTCTACGATCGATTGGCCGAGGAAGTCAAATCGAAGATCAAAAGGCTCGAGAATCCGGACCCGAGGTTCCTCAAGTACGGGTCGCCCCACCCGAGTCTCACGGACCACAGCCACATCCTCACCGCACCCGAGACACGTGTCACCACGCTCCCCAACGGCCTCCGGGTCGCCACCGAGTCGAACCTCGCGTCTAAGACCGCCACCGTCGGGGTCTGGATCGACGCCGGGTCAAGGTTCGAGAACGACGAGACCAACGGCACCGCACATTTCTTGGAGCACATGATCTTCAAGGGGACGGAGCAGAGGACCGCGCGTGATCTCGAGGAGGAGGTCGAGAACATGGGAGGCCATTTGAATGCGTACACATCGAGGGAGCAGACCACGTACTATGCCAAGGTGCTCCATTCAGATGTGCCAAAAGCGCTTTCGATTTTAGCTGATATCTTGCAGAACTCCAAGTTCGATGATGACCGGATTTTGCGCGAGCGCGATGTGATTTTAAGGGAAATGGAGGAGGTTGAGAAACAACCCGATGAAGTCATTTTTGACCATCTGCATGCAACTGCATTCCAGTACTCTCCTCTAGCCAGAACTATTCTTGGACCTTCTGACAACATTAAGACCATCTCCAAAGAGCATCTTCAGAACTATATCAAAACACATTACACTGCTCCCAGAATGGTTATTGCTGCTTCAGGAGCTATTAAGCATGAAGATCTTGTTGGTTCAGTTAAGGGGTTGTTTACTAAACTGTCTGGAGATCCTGCCACGGCCAGTCAGTTGGTTGCGGAAGAACCTTCCTTTTTCACTGGTTCTGAGGTCAGAATTGTGGATGATGACTTGCCTTTGGCACATTTTGCGGTTGCATTCAGCGGAGCGTCTTGGACAGATCCAGACTCTATTCCTCTAATGGTCATGCAGGCAATGTTGGGCTCATGGAATAAAAATGTTGGTGGTGGAAAGCACATGGGTTCAGAGCTGGCACAGAGGGTTGCCATTAATGACATTGCAGAAAACTATATGGCTTTCAACACCAACTACAAGGATACTGGCTTATTTGGTGTATTTGCTACTGCTAAGGAGGATTGCTTAGATGATTTGGCCTATGCAATTATGTATGAGGTAACCAAGTTAGTTTATCGAGTTTCAGAAGCTGATGTTATTCGTGCGCGCAATCAGTTGAAATCTTCTCTCCTCCTTCACTTGGATGGGACAAGTGCCGTAGCTGAAGATATTGGACGCCAGCTACTTACATATGGTCGAAGAATCCCGTTAGCCGAAATGTGTGCTCGAATCGATTCTGTTGATGCAAGCACCATCAAACGTGTTGCAAACCGGTTTATCTATGACAGGGATATTGCAATTGCAGCCATGGGTCCCGTTACAAAATTGCCCGACTACAACTGGTTCAGACGCCGCACCTACTGGAACAGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

533

Amino Acids

59.21

Weight (kDa)

6.3

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 108 - 255 6e-56 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 262 - 447 1e-35 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 416
AccI GTMKAC 1 cut(s) 162
AccII CGCG 6 cut(s) 347, 470, 472, 642, 648, 1330
AccIII TCCGGA 1 cut(s) 214
AclWI GGATC 4 cut(s) 75, 383, 914, 1043
AcoI YGGCCR 2 cut(s) 174, 930
AcuI CTGAAG 3 cut(s) 785, 837, 1407
AcvI CACGTG 1 cut(s) 296
AcyI GRCGYC 3 cut(s) 381, 1399, 1573
AfaI GTAC 4 cut(s) 236, 521, 545, 734
AfeI AGCGCT 1 cut(s) 582
AfiI CCNNNNNNNGG 3 cut(s) 366, 390, 1363
AflIII ACRYGT 3 cut(s) 293, 295, 1483
AgeI ACCGGT 1 cut(s) 1494
AgsI TTSAA 4 cut(s) 448, 514, 1189, 1342
AhdI GACNNNNNGTC 1 cut(s) 385
AjiI CACGTC 1 cut(s) 111
AjuI GAANNNNNNNTTGG 4 cut(s) 568, 600, 864, 896
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
AluBI AGCT 7 cut(s) 32, 596, 860, 1138, 1313, 1385, 1405
AluI AGCT 7 cut(s) 32, 596, 860, 1138, 1313, 1385, 1405
Alw21I GWGCWC 3 cut(s) 438, 561, 1450
Alw26I GTCTC 3 cut(s) 260, 284, 401
AlwI GGATC 4 cut(s) 75, 383, 914, 1043
AlwNI CAGNNNCTG 1 cut(s) 1313
Ama87I CYCGRG 5 cut(s) 206, 220, 250, 287, 479
Aor13HI TCCGGA 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 582
AoxI GGCC 6 cut(s) 41, 174, 316, 505, 930, 1260
ApeKI GCWGC 4 cut(s) 59, 71, 848, 1526
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AseI ATTAAT 1 cut(s) 1158
AsiGI ACCGGT 1 cut(s) 1494
Asp700I GAANNNNTTC 1 cut(s) 1456
AspLEI GCGC 6 cut(s) 472, 583, 642, 648, 1330, 1332
AspS9I GGNCC 6 cut(s) 42, 217, 262, 465, 761, 1535
AsuC2I CCSGG 2 cut(s) 323, 385
AsuHPI GGTGA 3 cut(s) 87, 271, 292
AvaI CYCGRG 5 cut(s) 206, 220, 250, 287, 479
AvaII GGWCC 5 cut(s) 217, 262, 465, 761, 1535
BanI GGYRCC 1 cut(s) 416
BanII GRGCYC 1 cut(s) 1094
BbrPI CACGTG 1 cut(s) 296
Bbv12I GWGCWC 3 cut(s) 438, 561, 1450
BbvI GCAGC 4 cut(s) 46, 83, 835, 1538
BccI CCATC 5 cut(s) 133, 717, 791, 1361, 1484
BceAI ACGGC 7 cut(s) 56, 59, 86, 331, 430, 945, 1364
BcgI CGANNNNNNTGC 2 cut(s) 1427, 1461
BciVI GTATCC 1 cut(s) 1198
BcnI CCSGG 2 cut(s) 323, 385
BcoDI GTCTC 3 cut(s) 260, 284, 401
BfaI CTAG 2 cut(s) 29, 743
BfoI RGCGCY 1 cut(s) 584
BfuI GTATCC 1 cut(s) 1198
BglII AGATCT 1 cut(s) 874
BisI GCNGC 5 cut(s) 60, 72, 849, 1527, 1576
BlsI GCNGC 5 cut(s) 61, 73, 850, 1528, 1577
BmcAI AGTACT 1 cut(s) 734
Bme1390I CCNGG 2 cut(s) 323, 385
Bme18I GGWCC 5 cut(s) 217, 262, 465, 761, 1535
BmeRI GACNNNNNGTC 1 cut(s) 385
BmeT110I CYCGRG 5 cut(s) 206, 220, 250, 287, 479
BmgBI CACGTC 1 cut(s) 111
BmgT120I GGNCC 6 cut(s) 42, 217, 262, 465, 761, 1535
BmiI GGNNCC 6 cut(s) 44, 219, 227, 418, 1536, 1537
BmrFI CCNGG 2 cut(s) 323, 385
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 2 cut(s) 805, 1456
BmuI ACTGGG 1 cut(s) 79
BoxI GACNNNNGTC 1 cut(s) 296
BpmI CTGGAG 1 cut(s) 936
Bpu10I CCTNAGC 1 cut(s) 1236
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 2 cut(s) 323, 385
Bsa29I ATCGAT 2 cut(s) 169, 1455
BsaAI YACGTR 2 cut(s) 296, 543
BsaHI GRCGYC 3 cut(s) 381, 1399, 1573
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 6 cut(s) 156, 177, 221, 552, 927, 1530
BsaWI WCCGGW 3 cut(s) 214, 630, 1494
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 390, 1363
Bse118I RCCGGY 2 cut(s) 55, 1494
Bse1I ACTGG 7 cut(s) 85, 730, 933, 973, 1213, 1568, 1589
Bse3DI GCAATG 2 cut(s) 1089, 1164
BseAI TCCGGA 1 cut(s) 214
BseCI ATCGAT 2 cut(s) 169, 1455
BseDI CCNNGG 6 cut(s) 156, 177, 221, 552, 927, 1530
BseGI GGATG 5 cut(s) 119, 125, 273, 997, 1372
BseLI CCNNNNNNNGG 3 cut(s) 366, 390, 1363
BseMI GCAATG 2 cut(s) 1089, 1164
BseMII CTCAG 1 cut(s) 966
BseNI ACTGG 7 cut(s) 85, 730, 933, 973, 1213, 1568, 1589
BsePI GCGCGC 1 cut(s) 1328
BseRI GAGGAG 6 cut(s) 132, 497, 500, 686, 729, 1343
BseXI GCAGC 4 cut(s) 46, 83, 835, 1538
Bsh1236I CGCG 6 cut(s) 347, 470, 472, 642, 648, 1330
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 6 cut(s) 43, 176, 318, 507, 932, 1262
BshNI GGYRCC 1 cut(s) 416
BshTI ACCGGT 1 cut(s) 1494
BshVI ATCGAT 2 cut(s) 169, 1455
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 3 cut(s) 438, 561, 1450
BsiHKCI CYCGRG 5 cut(s) 206, 220, 250, 287, 479
BsiSI CCGG 6 cut(s) 56, 215, 322, 384, 631, 1495
BslFI GGGAC 3 cut(s) 466, 1384, 1521
BslI CCNNNNNNNGG 3 cut(s) 366, 390, 1363
BsmAI GTCTC 3 cut(s) 260, 284, 401
BsmFI GGGAC 3 cut(s) 466, 1384, 1521
BsmI GAATGC 3 cut(s) 520, 725, 1025
BsnI GGCC 6 cut(s) 43, 176, 318, 507, 932, 1262
Bso31I GGTCTC 1 cut(s) 401
BsoBI CYCGRG 5 cut(s) 206, 220, 250, 287, 479
Bsp1286I GDGCHC 4 cut(s) 438, 561, 1094, 1450
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 9 cut(s) 80, 166, 195, 375, 441, 475, 874, 919, 1048
Bsp19I CCATGG 2 cut(s) 156, 1530
BspANI GGCC 6 cut(s) 43, 176, 318, 507, 932, 1262
BspCNI CTCAG 1 cut(s) 967
BspDI ATCGAT 2 cut(s) 169, 1455
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 6 cut(s) 347, 470, 472, 642, 648, 1330
BspLI GGNNCC 6 cut(s) 44, 219, 227, 418, 1536, 1537
BspPI GGATC 4 cut(s) 75, 383, 914, 1043
BspT107I GGYRCC 1 cut(s) 416
BspTNI GGTCTC 1 cut(s) 401
BsrDI GCAATG 2 cut(s) 1089, 1164
BsrFI RCCGGY 2 cut(s) 55, 1494
BsrI ACTGG 7 cut(s) 85, 730, 933, 973, 1213, 1568, 1589
BssAI RCCGGY 2 cut(s) 55, 1494
BssECI CCNNGG 6 cut(s) 156, 177, 221, 552, 927, 1530
BssHII GCGCGC 1 cut(s) 1328
BssMI GATC 9 cut(s) 80, 166, 195, 375, 441, 475, 874, 919, 1048
BssNI GRCGYC 3 cut(s) 381, 1399, 1573
BssT1I CCWWGG 3 cut(s) 156, 552, 1530
Bst4CI ACNGT 2 cut(s) 364, 912
BstACI GRCGYC 3 cut(s) 381, 1399, 1573
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstBAI YACGTR 2 cut(s) 296, 543
BstC8I GCNNGC 9 cut(s) 34, 57, 644, 717, 1080, 1140, 1330, 1403, 1471
BstDEI CTNAG 4 cut(s) 351, 975, 1236, 1249
BstDSI CCRYGG 3 cut(s) 156, 927, 1530
BstEII GGTNACC 1 cut(s) 1282
BstF5I GGATG 5 cut(s) 119, 125, 273, 997, 1372
BstFNI CGCG 6 cut(s) 347, 470, 472, 642, 648, 1330
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 6 cut(s) 472, 583, 642, 648, 1330, 1332
BstKTI GATC 9 cut(s) 83, 169, 198, 378, 444, 478, 877, 922, 1051
BstMAI GTCTC 3 cut(s) 260, 284, 401
BstMBI GATC 9 cut(s) 80, 166, 195, 375, 441, 475, 874, 919, 1048
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 5 cut(s) 68, 580, 857, 1016, 1526
BstNSI RCATGY 1 cut(s) 719
BstPAI GACNNNNGTC 1 cut(s) 296
BstPI GGTNACC 1 cut(s) 1282
BstSCI CCNGG 2 cut(s) 321, 383
BstUI CGCG 6 cut(s) 347, 470, 472, 642, 648, 1330
BstV1I GCAGC 4 cut(s) 46, 83, 835, 1538
BstX2I RGATCY 3 cut(s) 874, 919, 1048
BstXI CCANNNNNNTGG 1 cut(s) 940
BstYI RGATCY 3 cut(s) 874, 919, 1048
Bsu15I ATCGAT 2 cut(s) 169, 1455
BsuI GTATCC 1 cut(s) 1198
BsuRI GGCC 6 cut(s) 43, 176, 318, 507, 932, 1262
BsuTUI ATCGAT 2 cut(s) 169, 1455
BtgI CCRYGG 3 cut(s) 156, 927, 1530
BtgZI GCGATG 2 cut(s) 20, 663
BtrI CACGTC 1 cut(s) 111
BtsCI GGATG 5 cut(s) 119, 125, 273, 997, 1372
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 2 cut(s) 825, 966
Cac8I GCNNGC 9 cut(s) 34, 57, 644, 717, 1080, 1140, 1330, 1403, 1471
CaiI CAGNNNCTG 1 cut(s) 1313
CfoI GCGC 6 cut(s) 472, 583, 642, 648, 1330, 1332
Cfr10I RCCGGY 2 cut(s) 55, 1494
Cfr13I GGNCC 6 cut(s) 42, 217, 262, 465, 761, 1535
ClaI ATCGAT 2 cut(s) 169, 1455
CseI GACGC 5 cut(s) 336, 389, 1026, 1407, 1581
Csp6I GTAC 4 cut(s) 235, 520, 544, 733
CspAI ACCGGT 1 cut(s) 1494
CviAII CATG 9 cut(s) 157, 439, 499, 716, 869, 1075, 1095, 1126, 1531
CviQI GTAC 4 cut(s) 235, 520, 544, 733
DdeI CTNAG 4 cut(s) 351, 975, 1236, 1249
DpnI GATC 9 cut(s) 82, 168, 197, 377, 443, 477, 876, 921, 1050
DpnII GATC 9 cut(s) 80, 166, 195, 375, 441, 475, 874, 919, 1048
DriI GACNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 2 cut(s) 174, 930
Eam1105I GACNNNNNGTC 1 cut(s) 385
Eco130I CCWWGG 3 cut(s) 156, 552, 1530
Eco24I GRGCYC 1 cut(s) 1094
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 601
Eco47I GGWCC 5 cut(s) 217, 262, 465, 761, 1535
Eco47III AGCGCT 1 cut(s) 582
Eco57I CTGAAG 3 cut(s) 785, 837, 1407
Eco72I CACGTG 1 cut(s) 296
Eco88I CYCGRG 5 cut(s) 206, 220, 250, 287, 479
Eco91I GGTNACC 1 cut(s) 1282
EcoO109I RGGNCCY 1 cut(s) 1535
EcoO65I GGTNACC 1 cut(s) 1282
EcoRV GATATC 1 cut(s) 601
EcoT14I CCWWGG 3 cut(s) 156, 552, 1530
EcoT38I GRGCYC 1 cut(s) 1094
ErhI CCWWGG 3 cut(s) 156, 552, 1530
FaeI CATG 9 cut(s) 160, 442, 502, 719, 872, 1078, 1098, 1129, 1534
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FaqI GGGAC 3 cut(s) 466, 1384, 1521
FatI CATG 9 cut(s) 156, 438, 498, 715, 868, 1074, 1094, 1125, 1530
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 1414
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 5 cut(s) 60, 72, 849, 1527, 1576
FokI GGATG 5 cut(s) 106, 112, 260, 1004, 1379
FriOI GRGCYC 1 cut(s) 1094
Fsp4HI GCNGC 5 cut(s) 60, 72, 849, 1527, 1576
FspBI CTAG 2 cut(s) 29, 743
GlaI GCGC 6 cut(s) 471, 582, 641, 647, 1329, 1331
GluI GCNGC 5 cut(s) 60, 72, 849, 1527, 1576
GsuI CTGGAG 1 cut(s) 936
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 6 cut(s) 43, 176, 318, 507, 932, 1262
HapII CCGG 6 cut(s) 56, 215, 322, 384, 631, 1495
HgaI GACGC 5 cut(s) 336, 389, 1026, 1407, 1581
HhaI GCGC 6 cut(s) 472, 583, 642, 648, 1330, 1332
Hin1I GRCGYC 3 cut(s) 381, 1399, 1573
Hin1II CATG 9 cut(s) 160, 442, 502, 719, 872, 1078, 1098, 1129, 1534
Hin6I GCGC 6 cut(s) 470, 581, 640, 646, 1328, 1330
HinP1I GCGC 6 cut(s) 470, 581, 640, 646, 1328, 1330
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HinfI GANTC 7 cut(s) 211, 253, 335, 1055, 1425, 1452, 1457
HpaII CCGG 6 cut(s) 56, 215, 322, 384, 631, 1495
HphI GGTGA 3 cut(s) 87, 271, 292
Hpy166II GTNNAC 4 cut(s) 90, 163, 522, 903
Hpy188I TCNGA 8 cut(s) 568, 769, 804, 976, 983, 1135, 1309, 1571
Hpy188III TCNNGA 9 cut(s) 208, 215, 373, 397, 479, 493, 855, 915, 1052
Hpy8I GTNNAC 4 cut(s) 90, 163, 522, 903
Hpy99I CGWCG 5 cut(s) 42, 107, 368, 383, 407
HpyAV CCTTC 4 cut(s) 26, 774, 966, 1367
HpyCH4III ACNGT 2 cut(s) 364, 912
HpyCH4IV ACGT 4 cut(s) 110, 295, 542, 1483
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 580, 857, 1016, 1526
HpyF3I CTNAG 4 cut(s) 351, 975, 1236, 1249
HpySE526I ACGT 4 cut(s) 110, 295, 542, 1483
Hsp92I GRCGYC 3 cut(s) 381, 1399, 1573
Hsp92II CATG 9 cut(s) 160, 442, 502, 719, 872, 1078, 1098, 1129, 1534
HspAI GCGC 6 cut(s) 470, 581, 640, 646, 1328, 1330
KflI GGGWCCC 1 cut(s) 1535
Kpn2I TCCGGA 1 cut(s) 214
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Kzo9I GATC 9 cut(s) 80, 166, 195, 375, 441, 475, 874, 919, 1048
LmnI GCTCC 8 cut(s) 312, 433, 457, 532, 564, 835, 857, 1034
Lsp1109I GCAGC 4 cut(s) 46, 83, 835, 1538
LweI GCATC 2 cut(s) 805, 1456
MaeI CTAG 2 cut(s) 29, 743
MaeII ACGT 4 cut(s) 110, 295, 542, 1483
MaeIII GTNAC 3 cut(s) 298, 1282, 1540
MalI GATC 9 cut(s) 82, 168, 197, 377, 443, 477, 876, 921, 1050
MboI GATC 9 cut(s) 80, 166, 195, 375, 441, 475, 874, 919, 1048
MboII GAAGA 9 cut(s) 108, 205, 436, 791, 884, 962, 1338, 1400, 1434
MfeI CAATTG 1 cut(s) 1521
MflI RGATCY 3 cut(s) 874, 919, 1048
MhlI GDGCHC 4 cut(s) 438, 561, 1094, 1450
MluCI AATT 4 cut(s) 984, 1269, 1521, 1547
MlyI GAGTC 3 cut(s) 262, 344, 1049
MroI TCCGGA 1 cut(s) 214
MroNI GCCGGC 1 cut(s) 55
MroXI GAANNNNTTC 1 cut(s) 1456
MseI TTAA 5 cut(s) 659, 777, 864, 891, 1158
MslI CAYNNNNRTG 4 cut(s) 513, 567, 714, 1365
MspA1I CMGCKG 1 cut(s) 1032
MspI CCGG 6 cut(s) 56, 215, 322, 384, 631, 1495
MspR9I CCNGG 2 cut(s) 323, 385
MunI CAATTG 1 cut(s) 1521
Mva1269I GAATGC 3 cut(s) 520, 725, 1025
MvnI CGCG 6 cut(s) 347, 470, 472, 642, 648, 1330
MwoI GCNNNNNNNGC 5 cut(s) 68, 580, 857, 1016, 1526
NaeI GCCGGC 1 cut(s) 57
NciI CCSGG 2 cut(s) 323, 385
NcoI CCATGG 2 cut(s) 156, 1530
NdeI CATATG 1 cut(s) 1414
NdeII GATC 9 cut(s) 80, 166, 195, 375, 441, 475, 874, 919, 1048
NgoMIV GCCGGC 1 cut(s) 55
NlaIII CATG 9 cut(s) 160, 442, 502, 719, 872, 1078, 1098, 1129, 1534
NlaIV GGNNCC 6 cut(s) 44, 219, 227, 418, 1536, 1537
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 298
NspI RCATGY 1 cut(s) 719
PaeI GCATGC 1 cut(s) 719
PaeR7I CTCGAG 2 cut(s) 206, 479
PauI GCGCGC 1 cut(s) 1328
PcsI WCGNNNNNNNCGW 3 cut(s) 248, 344, 402
PctI GAATGC 3 cut(s) 520, 725, 1025
PdiI GCCGGC 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 1456
PfeI GAWTC 4 cut(s) 211, 1425, 1452, 1457
PinAI ACCGGT 1 cut(s) 1494
PkrI GCNGC 5 cut(s) 61, 73, 850, 1528, 1577
Ple19I CGATCG 1 cut(s) 169
PleI GAGTC 3 cut(s) 261, 343, 1049
PmaCI CACGTG 1 cut(s) 296
PmlI CACGTG 1 cut(s) 296
PpsI GAGTC 3 cut(s) 261, 343, 1049
Ppu21I YACGTR 2 cut(s) 296, 543
PpuMI RGGWCCY 1 cut(s) 1535
PshAI GACNNNNGTC 1 cut(s) 296
PshBI ATTAAT 1 cut(s) 1158
Psp5II RGGWCCY 1 cut(s) 1535
PspCI CACGTG 1 cut(s) 296
PspEI GGTNACC 1 cut(s) 1282
PspN4I GGNNCC 6 cut(s) 44, 219, 227, 418, 1536, 1537
PspPI GGNCC 6 cut(s) 42, 217, 262, 465, 761, 1535
PspPPI RGGWCCY 1 cut(s) 1535
PstNI CAGNNNCTG 1 cut(s) 1313
PsuI RGATCY 3 cut(s) 874, 919, 1048
PteI GCGCGC 1 cut(s) 1328
PvuI CGATCG 1 cut(s) 169
RsaI GTAC 4 cut(s) 236, 521, 545, 734
RsaNI GTAC 4 cut(s) 235, 520, 544, 733
RseI CAYNNNNRTG 4 cut(s) 513, 567, 714, 1365
SaqAI TTAA 5 cut(s) 659, 777, 864, 891, 1158
SatI GCNGC 5 cut(s) 60, 72, 849, 1527, 1576
Sau3AI GATC 9 cut(s) 80, 166, 195, 375, 441, 475, 874, 919, 1048
Sau96I GGNCC 6 cut(s) 42, 217, 262, 465, 761, 1535
ScaI AGTACT 1 cut(s) 734
SchI GAGTC 3 cut(s) 262, 344, 1049
ScrFI CCNGG 2 cut(s) 323, 385
SduI GDGCHC 4 cut(s) 438, 561, 1094, 1450
SfaNI GCATC 2 cut(s) 805, 1456
Sfr274I CTCGAG 2 cut(s) 206, 479
SinI GGWCC 5 cut(s) 217, 262, 465, 761, 1535
SlaI CTCGAG 2 cut(s) 206, 479
SmiMI CAYNNNNRTG 4 cut(s) 513, 567, 714, 1365
SmlI CTYRAG 3 cut(s) 206, 230, 479
SmoI CTYRAG 3 cut(s) 206, 230, 479
SphI GCATGC 1 cut(s) 719
Sse9I AATT 4 cut(s) 984, 1269, 1521, 1547
SspMI CTAG 2 cut(s) 29, 743
StyD4I CCNGG 2 cut(s) 321, 383
StyI CCWWGG 3 cut(s) 156, 552, 1530
TaaI ACNGT 2 cut(s) 364, 912
TaiI ACGT 4 cut(s) 113, 298, 545, 1486
TasI AATT 4 cut(s) 984, 1269, 1521, 1547
TatI WGTACW 1 cut(s) 732
TauI GCSGC 1 cut(s) 1578
TfiI GAWTC 4 cut(s) 211, 1425, 1452, 1457
Tru1I TTAA 5 cut(s) 659, 777, 864, 891, 1158
Tru9I TTAA 5 cut(s) 659, 777, 864, 891, 1158
TscAI CASTG 2 cut(s) 832, 973
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 4 cut(s) 59, 71, 848, 1526
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 4 cut(s) 23, 108, 708, 885
TspGWI ACGGA 2 cut(s) 275, 470
TspRI CASTG 2 cut(s) 832, 973
VpaK11BI GGWCC 5 cut(s) 217, 262, 465, 761, 1535
VspI ATTAAT 1 cut(s) 1158
XceI RCATGY 1 cut(s) 719
XhoI CTCGAG 2 cut(s) 206, 479
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 1456
XspI CTAG 2 cut(s) 29, 743
ZrmI AGTACT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.