FvH4_6g49140
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011709
Physical Location & Seq
Reverse (-)
36880282 .. 36882397
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49140.t2

Sequence Viewer

Length: 1377 bp
ATGCCAAACTCTGATTCAACAGCAGCAGCAGCTGTTGGAACGAAGGTTGAATCCTTCGGCTACCAAGAAGGGCAGGAGAATTATGAACTTGTTAATGCCAAAGCCGAAATGAGTGAGGTCAGAGAAGAAAATGCGAGACTGAAGAAGACGTTGGAGCATATGACGAAGGATTACCAGTCTCTACAGTTGCGCTTTTTTGACATCCTCAAACAAGAGTCTCATTCCAAGAAGGCAACAATGAGTGCCACCACCTCCCATGTTGAAATTGAAGAACCTCATGATCAGCTCTTGTCTCTTTGCCTTGGAAGAAGCCCAAGAGACCCTAAAAATGTTAGTGATGAGGGCAAACTTGTACAAGCTGATCATCACCAAGACTCGAAGGCCAACCTTACTCTTGGATTAGGCTCTAAGTTGCAGTTGCCTACCGAGCTTGTATTGAGCGGTCCGATTCCGGAGACTAGTTCCGAAGAGCCTAAGGAATCAGAAGCCTCCCCATCAAGTACTAAAACACCAAAGACAATAAGAAATGAAGATGATGATGCTTCGCAGCAGGCCAATGCTGCAAAAAGAGCTAGGGTTTCTGTGAGAGCTCGATGTGACACCCCCACGATGATTGATGGATGCCAATGGAGAAAATACGGACAGAAGATTGCGAAAGGAAATCCATGCCCGCGAGCATACTACCGATGCACGGTTGCACCAGGATGCCCTGTTAGAAAGCAGGTGCAAAGATGTATTGATGACATGTCTATCTTGATCACCACCTATGAAGGAACCCACAACCACCCACTTCCAATGACAGCCACTTCCATGGCTTCCACCACCTCTGCCGCAGCTTCCATGTTATTGTCTGGCTCTTTGACATCTCAGCTTCCAGTTACTCTAGACCTCACATCTTCTCCTTCCCCTTCATCAAACCAATACTGCACATTTCCTCCAAGTCTAAGCTTTTGTTCCTCAGAATCTAACAGCAATTTACCACCCAGTTGGGGCAACGTGTACCCCAGATTTGGGACGCAGTTTGACAGAAAGAACCAAGAGTGTTTCCAACCCAATCACCAAGCCTCTTCCCAGGTGTCCTTAACAGAGACATTGACCAAGGCAATCACATCAGACCCAAATTTCAAGTCAGTCATTGCTGTAGCGCTTTCATCTATGGTTGGAGGTGGTGCAGCAAGTTATGGAAGCCAAGGAATAGGGGAAAGATTAGGACAACACTTGACACAGACTGGGAAAAGCCGCTCATCAAGCATTTTCAACAGCTTGTCATCTTCTGATTCTCAGAAGGGAAACTTGACGCTTCTCCAGCCTCCACTGCCATTTCCCGAGTCCAAGAGCACTACATCTTCCCCTGCCATCAGGGATAAAAGTCTCTAA

Protein Analysis

459

Amino Acids

49.6

Weight (kDa)

7.12

Isoelectric Point (pI)

54.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 206 - 263 9.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011709)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15130 AT5G15130
fragaria_vesca FvH4_6g49140 FvH4_6g49140
malus_domestica MD09G1048200.v1.1 MD09G1048300.v1.1 MD17G1048400.v1.1
prunus_persica Prupe.3G270800_v2.0.a1
pyrus_communis pycom111g03910
rosa_chinensis RchiOBHm_Chr2g0169011
rosa_laevigata RLG00000021823
rosa_multiflora Rmu_ssc0000393.1_g000021
rosa_roxburghii Rroxscaffold_2G00082540
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG607700 Rh2BG620200 Rh2CG589200 Rh2DG631000
rosa_wichuraiana Rw2G050390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 712
Acc36I ACCTGC 1 cut(s) 712
AccBSI CCGCTC 2 cut(s) 441, 1242
AccII CGCG 1 cut(s) 673
AccIII TCCGGA 1 cut(s) 451
AciI CCGC 4 cut(s) 441, 671, 831, 1240
AcsI RAATTY 1 cut(s) 1120
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 3 cut(s) 354, 502, 1001
AfeI AGCGCT 1 cut(s) 1146
AfiI CCNNNNNNNGG 4 cut(s) 691, 989, 1010, 1011
AflIII ACRYGT 2 cut(s) 744, 996
AgsI TTSAA 6 cut(s) 18, 50, 263, 269, 1126, 1258
AhlI ACTAGT 1 cut(s) 458
AjnI CCWGG 2 cut(s) 700, 1071
AjuI GAANNNNNNNTTGG 3 cut(s) 30, 134, 166
Alw21I GWGCWC 2 cut(s) 592, 1340
Alw26I GTCTC 7 cut(s) 130, 183, 222, 297, 312, 449, 1082
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 1325
Aor13HI TCCGGA 1 cut(s) 451
Aor51HI AGCGCT 1 cut(s) 1146
AoxI GGCC 2 cut(s) 381, 552
ApeKI GCWGC 7 cut(s) 23, 26, 29, 547, 560, 833, 1172
ApoI RAATTY 1 cut(s) 1120
ArsI GACNNNNNNTTYG 2 cut(s) 1112, 1144
AspLEI GCGC 2 cut(s) 192, 1147
AspS9I GGNCC 1 cut(s) 443
AsuHPI GGTGA 3 cut(s) 359, 751, 1049
AvaI CYCGRG 1 cut(s) 1325
AvaII GGWCC 1 cut(s) 443
AxyI CCTNAGG 1 cut(s) 474
BanII GRGCYC 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 152
Bbv12I GWGCWC 2 cut(s) 592, 1340
BbvI GCAGC 7 cut(s) 35, 38, 41, 547, 559, 845, 1184
BccI CCATC 3 cut(s) 502, 611, 1364
BciT130I CCWGG 2 cut(s) 702, 1073
BclI TGATCA 3 cut(s) 280, 361, 756
BcoDI GTCTC 7 cut(s) 130, 183, 222, 297, 312, 449, 1082
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 3 cut(s) 459, 573, 884
BfmI CTRYAG 2 cut(s) 182, 1140
BfoI RGCGCY 1 cut(s) 1148
BfuAI ACCTGC 1 cut(s) 712
BisI GCNGC 9 cut(s) 24, 27, 30, 548, 561, 831, 834, 1173, 1240
BlsI GCNGC 9 cut(s) 25, 28, 31, 549, 562, 832, 835, 1174, 1241
BmcAI AGTACT 1 cut(s) 502
Bme1390I CCNGG 2 cut(s) 702, 1073
Bme18I GGWCC 1 cut(s) 443
BmeT110I CYCGRG 1 cut(s) 1325
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 1 cut(s) 775
BmrFI CCNGG 2 cut(s) 702, 1073
BmrI ACTGGG 2 cut(s) 978, 1239
BmsI GCATC 4 cut(s) 529, 611, 677, 695
BmuI ACTGGG 2 cut(s) 978, 1239
BpiI GAAGAC 1 cut(s) 152
BpmI CTGGAG 1 cut(s) 1289
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 5 cut(s) 301, 810, 1071, 1098, 1189
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 4 cut(s) 883, 913, 919, 949
Bsc4I CCNNNNNNNGG 4 cut(s) 691, 989, 1010, 1011
Bse1I ACTGG 4 cut(s) 175, 875, 984, 1234
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 1134
BseAI TCCGGA 1 cut(s) 451
BseBI CCWGG 2 cut(s) 702, 1073
BseDI CCNNGG 5 cut(s) 301, 810, 1071, 1098, 1189
BseGI GGATG 3 cut(s) 201, 626, 710
BseLI CCNNNNNNNGG 4 cut(s) 691, 989, 1010, 1011
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 3 cut(s) 881, 972, 1295
BseNI ACTGG 4 cut(s) 175, 875, 984, 1234
BseXI GCAGC 7 cut(s) 35, 38, 41, 547, 559, 845, 1184
BsgI GTGCAG 2 cut(s) 910, 1191
Bsh1236I CGCG 1 cut(s) 673
BshFI GGCC 2 cut(s) 383, 554
BsiHKAI GWGCWC 2 cut(s) 592, 1340
BsiHKCI CYCGRG 1 cut(s) 1325
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 1027
BslI CCNNNNNNNGG 4 cut(s) 691, 989, 1010, 1011
BsmAI GTCTC 7 cut(s) 130, 183, 222, 297, 312, 449, 1082
BsmFI GGGAC 1 cut(s) 1027
BsnI GGCC 2 cut(s) 383, 554
Bso31I GGTCTC 1 cut(s) 312
BsoBI CYCGRG 1 cut(s) 1325
Bsp1286I GDGCHC 2 cut(s) 592, 1340
Bsp13I TCCGGA 1 cut(s) 451
Bsp1407I TGTACA 1 cut(s) 352
Bsp143I GATC 3 cut(s) 280, 361, 756
Bsp19I CCATGG 1 cut(s) 810
BspACI CCGC 4 cut(s) 441, 671, 831, 1240
BspANI GGCC 2 cut(s) 383, 554
BspCNI CTCAG 3 cut(s) 880, 971, 1294
BspEI TCCGGA 1 cut(s) 451
BspFNI CGCG 1 cut(s) 673
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 775
BspMI ACCTGC 1 cut(s) 712
BspQI GCTCTTC 1 cut(s) 462
BspTNI GGTCTC 1 cut(s) 312
BsrBI CCGCTC 2 cut(s) 441, 1242
BsrDI GCAATG 1 cut(s) 1134
BsrGI TGTACA 1 cut(s) 352
BsrI ACTGG 4 cut(s) 175, 875, 984, 1234
BssECI CCNNGG 5 cut(s) 301, 810, 1071, 1098, 1189
BssMI GATC 3 cut(s) 280, 361, 756
BssT1I CCWWGG 4 cut(s) 301, 810, 1098, 1189
Bst2UI CCWGG 2 cut(s) 702, 1073
Bst4CI ACNGT 2 cut(s) 186, 694
Bst6I CTCTTC 2 cut(s) 462, 1072
BstAUI TGTACA 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 552, 671, 675
BstDEI CTNAG 6 cut(s) 408, 474, 867, 944, 958, 1281
BstDSI CCRYGG 1 cut(s) 810
BstF5I GGATG 3 cut(s) 201, 626, 710
BstFNI CGCG 1 cut(s) 673
BstH2I RGCGCY 1 cut(s) 1148
BstHHI GCGC 2 cut(s) 192, 1147
BstKTI GATC 3 cut(s) 283, 364, 759
BstMAI GTCTC 7 cut(s) 130, 183, 222, 297, 312, 449, 1082
BstMBI GATC 3 cut(s) 280, 361, 756
BstMWI GCNNNNNNNGC 7 cut(s) 29, 427, 560, 569, 1248, 1306, 1315
BstNI CCWGG 2 cut(s) 702, 1073
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 2 cut(s) 700, 1071
BstSFI CTRYAG 2 cut(s) 182, 1140
BstUI CGCG 1 cut(s) 673
BstV1I GCAGC 7 cut(s) 35, 38, 41, 547, 559, 845, 1184
BstV2I GAAGAC 1 cut(s) 152
BstXI CCANNNNNNTGG 2 cut(s) 811, 987
Bsu36I CCTNAGG 1 cut(s) 474
BsuRI GGCC 2 cut(s) 383, 554
BtgI CCRYGG 1 cut(s) 810
BtsCI GGATG 3 cut(s) 201, 626, 710
BtsI GCAGTG 1 cut(s) 1313
BtsIMutI CAGTG 1 cut(s) 1313
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 3 cut(s) 552, 671, 675
CaiI CAGNNNCTG 1 cut(s) 32
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 192, 1147
Cfr13I GGNCC 1 cut(s) 443
CpoI CGGWCCG 1 cut(s) 443
CseI GACGC 2 cut(s) 1024, 1306
Csp6I GTAC 3 cut(s) 353, 501, 1000
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CspI CGGWCCG 1 cut(s) 443
CviAII CATG 6 cut(s) 257, 278, 666, 745, 811, 841
CviQI GTAC 3 cut(s) 353, 501, 1000
DdeI CTNAG 6 cut(s) 408, 474, 867, 944, 958, 1281
DpnI GATC 3 cut(s) 282, 363, 758
DpnII GATC 3 cut(s) 280, 361, 756
Eam1104I CTCTTC 2 cut(s) 462, 1072
EarI CTCTTC 2 cut(s) 462, 1072
Ecl136II GAGCTC 1 cut(s) 590
Eco130I CCWWGG 4 cut(s) 301, 810, 1098, 1189
Eco24I GRGCYC 1 cut(s) 592
Eco31I GGTCTC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 443
Eco47III AGCGCT 1 cut(s) 1146
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 161
Eco81I CCTNAGG 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 1325
EcoICRI GAGCTC 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 700, 1071
EcoT14I CCWWGG 4 cut(s) 301, 810, 1098, 1189
EcoT38I GRGCYC 1 cut(s) 592
ErhI CCWWGG 4 cut(s) 301, 810, 1098, 1189
FaeI CATG 6 cut(s) 260, 281, 669, 748, 814, 844
FalI AAGNNNNNCTT 2 cut(s) 1277, 1309
FaqI GGGAC 1 cut(s) 1027
FatI CATG 6 cut(s) 256, 277, 665, 744, 810, 840
FauI CCCGC 1 cut(s) 678
FauNDI CATATG 1 cut(s) 159
FbaI TGATCA 3 cut(s) 280, 361, 756
Fnu4HI GCNGC 9 cut(s) 24, 27, 30, 548, 561, 831, 834, 1173, 1240
FokI GGATG 3 cut(s) 188, 633, 717
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 9 cut(s) 24, 27, 30, 548, 561, 831, 834, 1173, 1240
FspBI CTAG 3 cut(s) 459, 573, 884
GlaI GCGC 2 cut(s) 191, 1146
GluI GCNGC 9 cut(s) 24, 27, 30, 548, 561, 831, 834, 1173, 1240
GsuI CTGGAG 1 cut(s) 1289
HaeII RGCGCY 1 cut(s) 1148
HaeIII GGCC 2 cut(s) 383, 554
HapII CCGG 1 cut(s) 452
HgaI GACGC 2 cut(s) 1024, 1306
HhaI GCGC 2 cut(s) 192, 1147
Hin1II CATG 6 cut(s) 260, 281, 669, 748, 814, 844
Hin6I GCGC 2 cut(s) 190, 1145
HinP1I GCGC 2 cut(s) 190, 1145
HindIII AAGCTT 1 cut(s) 946
HinfI GANTC 9 cut(s) 14, 50, 215, 374, 448, 479, 962, 1277, 1328
HpaII CCGG 1 cut(s) 452
HphI GGTGA 3 cut(s) 359, 751, 1049
Hpy166II GTNNAC 1 cut(s) 1000
Hpy188I TCNGA 9 cut(s) 13, 122, 447, 466, 484, 961, 1114, 1276, 1284
Hpy188III TCNNGA 5 cut(s) 278, 452, 754, 884, 1325
Hpy8I GTNNAC 1 cut(s) 1000
HpyCH4III ACNGT 2 cut(s) 186, 694
HpyCH4IV ACGT 2 cut(s) 149, 996
HpyCH4V TGCA 7 cut(s) 415, 563, 690, 698, 727, 927, 1172
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 427, 560, 569, 1248, 1306, 1315
HpyF3I CTNAG 6 cut(s) 408, 474, 867, 944, 958, 1281
HpySE526I ACGT 2 cut(s) 149, 996
Hsp92II CATG 6 cut(s) 260, 281, 669, 748, 814, 844
HspAI GCGC 2 cut(s) 190, 1145
Kpn2I TCCGGA 1 cut(s) 451
Ksp22I TGATCA 3 cut(s) 280, 361, 756
Kzo9I GATC 3 cut(s) 280, 361, 756
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 1 cut(s) 154
Lsp1109I GCAGC 7 cut(s) 35, 38, 41, 547, 559, 845, 1184
LweI GCATC 4 cut(s) 529, 611, 677, 695
MaeI CTAG 3 cut(s) 459, 573, 884
MaeII ACGT 2 cut(s) 149, 996
MaeIII GTNAC 2 cut(s) 596, 877
MalI GATC 3 cut(s) 282, 363, 758
MbiI CCGCTC 2 cut(s) 441, 1242
MboI GATC 3 cut(s) 280, 361, 756
MhlI GDGCHC 2 cut(s) 592, 1340
MluCI AATT 4 cut(s) 79, 264, 973, 1120
MlyI GAGTC 3 cut(s) 224, 368, 1337
MmeI TCCRAC 4 cut(s) 16, 132, 1072, 1141
MroI TCCGGA 1 cut(s) 451
MseI TTAA 2 cut(s) 93, 1082
MslI CAYNNNNRTG 2 cut(s) 703, 809
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 2 cut(s) 702, 1073
MvaI CCWGG 2 cut(s) 702, 1073
MvnI CGCG 1 cut(s) 673
MwoI GCNNNNNNNGC 7 cut(s) 29, 427, 560, 569, 1248, 1306, 1315
NcoI CCATGG 1 cut(s) 810
NdeI CATATG 1 cut(s) 159
NdeII GATC 3 cut(s) 280, 361, 756
NlaIII CATG 6 cut(s) 260, 281, 669, 748, 814, 844
NlaIV GGNNCC 1 cut(s) 775
NmuCI GTSAC 1 cut(s) 596
NspI RCATGY 1 cut(s) 748
PagI TCATGA 1 cut(s) 277
PaqCI CACCTGC 1 cut(s) 712
PciI ACATGT 1 cut(s) 744
PciSI GCTCTTC 1 cut(s) 462
PfeI GAWTC 6 cut(s) 14, 50, 448, 479, 962, 1277
PkrI GCNGC 9 cut(s) 25, 28, 31, 549, 562, 832, 835, 1174, 1241
PleI GAGTC 3 cut(s) 223, 368, 1336
PpsI GAGTC 3 cut(s) 223, 368, 1336
PscI ACATGT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 700, 1071
PspGI CCWGG 2 cut(s) 700, 1071
PspN4I GGNNCC 1 cut(s) 775
PspPI GGNCC 1 cut(s) 443
PstNI CAGNNNCTG 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 3 cut(s) 354, 502, 1001
RsaNI GTAC 3 cut(s) 353, 501, 1000
RseI CAYNNNNRTG 2 cut(s) 703, 809
Rsr2I CGGWCCG 1 cut(s) 443
RsrII CGGWCCG 1 cut(s) 443
SacI GAGCTC 1 cut(s) 592
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 93, 1082
SatI GCNGC 9 cut(s) 24, 27, 30, 548, 561, 831, 834, 1173, 1240
Sau3AI GATC 3 cut(s) 280, 361, 756
Sau96I GGNCC 1 cut(s) 443
ScaI AGTACT 1 cut(s) 502
SchI GAGTC 3 cut(s) 224, 368, 1337
ScrFI CCNGG 2 cut(s) 702, 1073
SduI GDGCHC 2 cut(s) 592, 1340
SfaNI GCATC 4 cut(s) 529, 611, 677, 695
SfcI CTRYAG 2 cut(s) 182, 1140
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 2 cut(s) 703, 809
SpeI ACTAGT 1 cut(s) 458
Sse9I AATT 4 cut(s) 79, 264, 973, 1120
SsiI CCGC 4 cut(s) 441, 671, 831, 1240
SspMI CTAG 3 cut(s) 459, 573, 884
SstI GAGCTC 1 cut(s) 592
StyD4I CCNGG 2 cut(s) 700, 1071
StyI CCWWGG 4 cut(s) 301, 810, 1098, 1189
TaaI ACNGT 2 cut(s) 186, 694
TaiI ACGT 2 cut(s) 152, 999
TaqI TCGA 2 cut(s) 377, 592
TasI AATT 4 cut(s) 79, 264, 973, 1120
TatI WGTACW 2 cut(s) 352, 500
TauI GCSGC 2 cut(s) 833, 1242
TfiI GAWTC 6 cut(s) 14, 50, 448, 479, 962, 1277
Tru1I TTAA 2 cut(s) 93, 1082
Tru9I TTAA 2 cut(s) 93, 1082
TscAI CASTG 1 cut(s) 1320
TseFI GTSAC 1 cut(s) 596
TseI GCWGC 7 cut(s) 23, 26, 29, 547, 560, 833, 1172
Tsp45I GTSAC 1 cut(s) 596
TspDTI ATGAA 5 cut(s) 99, 543, 783, 900, 1140
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 1 cut(s) 1320
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 1 cut(s) 1120
XbaI TCTAGA 1 cut(s) 883
XceI RCATGY 1 cut(s) 748
XspI CTAG 3 cut(s) 459, 573, 884
ZrmI AGTACT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.