Rmu_ssc0000393.1_g000021
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000393.1
OG0011709
Physical Location & Seq
Reverse (-)
124827 .. 126727
1901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000393.1_g000021.1.cds

Sequence Viewer

Length: 1473 bp
atgccaaactctaattcaactgcagcagctgaagaaacgaaggttgaatcctttggctgccaaggagggcacagtgaggaggagaattatgaacttgttaatgccaaagccgaaatgaatgaggtcagaaaagaaaatgcgaggctgaaaaagacgttagagcatatgatgaaggattaccagtccctacagttgcgctttttcgacatcctcaaacaagagtctcattccaagaaggctacaatgagtgccagcacttctcatatcaaaatcgaagaagctcatgatcagctcttgtctctttgccttggaagaagtccaagagaccctaaaaatattagtgatgagagcagacttgtacaagttgatcatcaccaagacttgaaggccaaccttagtcttgaattaggctctaaattgcagttgccgaccgagcttgtattgagcggtccaattcccgagaatagttccgaagagcctaaggaagcagaagcgtccccatcaagtacaaaaacaccaaggacaataggaaacgaagatgatgaagcttcgcagcaggccaatgctgcaaaaagagctagggtttccgtgagagctcgatgtgatacccccacgatgaatgatggatgccaatggagaaaatatggacagaagattgcgaaaggaaatccatgcccccgagcatactatcgatgcacggttgcaccagcatgccctgttagaaagcaggtgcaaagatgtgttgatgacatgtctatcttgatcaccacctacgaaggaacccacaaccatccacttccaatgacagccacttccattgcttccaccacctctgccgcggcttccatgttattgtccggttcttcgacatctcagcttccaggtcttggttccagttttggtctctttgataattccagacaacaacagctctacttgtctaaattttcctcatctcctttgctacccacggttactctagacctcacatcctctccatccccttcatcaacccaataccgcacatttcgtccaagtgttagcttttgttcctcagaatccaacatcaatttaccaaccagttggggcaatgggtaccctaaattcgggacaccgtttgacagaaagaaccaagagtgtttccaacccaatcaccaaccctcttctcaggtgtccttaacagagacattgactaaggcaatcacatcagacccaaatttcaagtcggtaattgctgtagcactttcatctatggttggaggtggtgctgcaagttatggaagccaaggaataggagaaagattagggcaacacttgccacagaatgcgaaaggctgcgcatcaagcattttcaacagtctgtcatcttctgattctcagaagggaaacttgacgcttctccagcctccactgccatttcccgagtccaagagcacttcgtcttcccctgccatcagggataaaagtctctaa

Protein Analysis

490

Amino Acids

53.17

Weight (kDa)

7.94

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011709)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15130 AT5G15130
fragaria_vesca FvH4_6g49140 FvH4_6g49140
malus_domestica MD09G1048200.v1.1 MD09G1048300.v1.1 MD17G1048400.v1.1
prunus_persica Prupe.3G270800_v2.0.a1
pyrus_communis pycom111g03910
rosa_chinensis RchiOBHm_Chr2g0169011
rosa_laevigata RLG00000021823
rosa_multiflora Rmu_ssc0000393.1_g000021
rosa_roxburghii Rroxscaffold_2G00082540
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG607700 Rh2BG620200 Rh2CG589200 Rh2DG631000
rosa_wichuraiana Rw2G050390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 718
Acc16I TGCGCA 1 cut(s) 1339
Acc36I ACCTGC 1 cut(s) 718
Acc65I GGTACC 1 cut(s) 1095
AccB1I GGYRCC 1 cut(s) 1095
AccB7I CCANNNNNTGG 1 cut(s) 887
AccBSI CCGCTC 1 cut(s) 447
AccII CGCG 1 cut(s) 839
AciI CCGC 4 cut(s) 447, 837, 839, 1021
AcsI RAATTY 3 cut(s) 944, 1103, 1216
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 3 cut(s) 360, 508, 1097
AfiI CCNNNNNNNGG 2 cut(s) 887, 1106
AflIII ACRYGT 1 cut(s) 750
AgsI TTSAA 6 cut(s) 18, 47, 385, 404, 1222, 1354
AjnI CCWGG 1 cut(s) 880
AjuI GAANNNNNNNTTGG 1 cut(s) 30
Alw21I GWGCWC 2 cut(s) 598, 1436
Alw26I GTCTC 5 cut(s) 228, 303, 318, 908, 1178
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 3 cut(s) 458, 678, 1421
AoxI GGCC 2 cut(s) 387, 558
ApeKI GCWGC 7 cut(s) 23, 26, 57, 553, 566, 1268, 1335
ApoI RAATTY 3 cut(s) 944, 1103, 1216
ArsI GACNNNNNNTTYG 2 cut(s) 1208, 1240
Asp718I GGTACC 1 cut(s) 1095
AspLEI GCGC 2 cut(s) 198, 1340
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 3 cut(s) 365, 757, 1145
AvaI CYCGRG 3 cut(s) 458, 678, 1421
AvaII GGWCC 1 cut(s) 449
AxyI CCTNAGG 1 cut(s) 480
BaeGI GKGCMC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 1095
BanII GRGCYC 1 cut(s) 598
BbsI GAAGAC 1 cut(s) 1434
Bbv12I GWGCWC 2 cut(s) 598, 1436
BbvI GCAGC 7 cut(s) 35, 38, 44, 553, 565, 1255, 1322
BccI CCATC 5 cut(s) 508, 617, 798, 1006, 1460
BciT130I CCWGG 1 cut(s) 882
BclI TGATCA 3 cut(s) 286, 367, 762
BcoDI GTCTC 5 cut(s) 228, 303, 318, 908, 1178
BfaI CTAG 2 cut(s) 579, 980
BfmI CTRYAG 3 cut(s) 21, 188, 1236
BfuAI ACCTGC 1 cut(s) 718
BisI GCNGC 9 cut(s) 24, 27, 58, 554, 567, 837, 840, 1269, 1336
BlsI GCNGC 9 cut(s) 25, 28, 59, 555, 568, 838, 841, 1270, 1337
Bme1390I CCNGG 1 cut(s) 882
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 3 cut(s) 458, 678, 1421
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 3 cut(s) 781, 892, 1097
BmrFI CCNGG 1 cut(s) 882
BmsI GCATC 3 cut(s) 617, 683, 1349
BpiI GAAGAC 1 cut(s) 1434
BpmI CTGGAG 1 cut(s) 1385
Bsa29I ATCGAT 1 cut(s) 691
BsaI GGTCTC 2 cut(s) 318, 908
BsaJI CCNNGG 6 cut(s) 61, 307, 518, 837, 969, 1285
BsaWI WCCGGW 1 cut(s) 857
BsaXI ACNNNNNCTCC 2 cut(s) 979, 1009
Bsc4I CCNNNNNNNGG 2 cut(s) 887, 1106
Bse1I ACTGG 3 cut(s) 181, 894, 1080
Bse21I CCTNAGG 1 cut(s) 480
Bse3DI GCAATG 2 cut(s) 816, 1096
BseBI CCWGG 1 cut(s) 882
BseCI ATCGAT 1 cut(s) 691
BseDI CCNNGG 6 cut(s) 61, 307, 518, 837, 969, 1285
BseGI GGATG 5 cut(s) 207, 632, 790, 989, 998
BseLI CCNNNNNNNGG 2 cut(s) 887, 1106
BseMI GCAATG 2 cut(s) 816, 1096
BseMII CTCAG 4 cut(s) 887, 1068, 1181, 1391
BseNI ACTGG 3 cut(s) 181, 894, 1080
BseRI GAGGAG 2 cut(s) 92, 95
BseSI GKGCMC 1 cut(s) 72
BseXI GCAGC 7 cut(s) 35, 38, 44, 553, 565, 1255, 1322
Bsh1236I CGCG 1 cut(s) 839
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 2 cut(s) 389, 560
BshNI GGYRCC 1 cut(s) 1095
BshVI ATCGAT 1 cut(s) 691
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 2 cut(s) 598, 1436
BsiHKCI CYCGRG 3 cut(s) 458, 678, 1421
BsiSI CCGG 1 cut(s) 858
BslFI GGGAC 3 cut(s) 169, 481, 1123
BslI CCNNNNNNNGG 2 cut(s) 887, 1106
BsmAI GTCTC 5 cut(s) 228, 303, 318, 908, 1178
BsmFI GGGAC 3 cut(s) 169, 481, 1123
BsmI GAATGC 1 cut(s) 1330
BsnI GGCC 2 cut(s) 389, 560
Bso31I GGTCTC 2 cut(s) 318, 908
BsoBI CYCGRG 3 cut(s) 458, 678, 1421
Bsp1286I GDGCHC 3 cut(s) 72, 598, 1436
Bsp1407I TGTACA 1 cut(s) 358
Bsp143I GATC 3 cut(s) 286, 367, 762
BspACI CCGC 4 cut(s) 447, 837, 839, 1021
BspANI GGCC 2 cut(s) 389, 560
BspCNI CTCAG 4 cut(s) 886, 1067, 1180, 1390
BspDI ATCGAT 1 cut(s) 691
BspFNI CGCG 1 cut(s) 839
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 3 cut(s) 781, 892, 1097
BspMAI CTGCAG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 718
BspQI GCTCTTC 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 1095
BspTNI GGTCTC 2 cut(s) 318, 908
BsrBI CCGCTC 1 cut(s) 447
BsrDI GCAATG 2 cut(s) 816, 1096
BsrGI TGTACA 1 cut(s) 358
BsrI ACTGG 3 cut(s) 181, 894, 1080
BssECI CCNNGG 6 cut(s) 61, 307, 518, 837, 969, 1285
BssMI GATC 3 cut(s) 286, 367, 762
BssT1I CCWWGG 4 cut(s) 61, 307, 518, 1285
Bst2UI CCWGG 1 cut(s) 882
Bst4CI ACNGT 6 cut(s) 74, 192, 700, 973, 1116, 1358
Bst6I CTCTTC 2 cut(s) 468, 1168
BstAPI GCANNNNNTGC 1 cut(s) 1315
BstAUI TGTACA 1 cut(s) 358
BstC8I GCNNGC 3 cut(s) 253, 558, 712
BstDEI CTNAG 7 cut(s) 395, 480, 873, 1054, 1167, 1194, 1377
BstDSI CCRYGG 2 cut(s) 837, 969
BstF5I GGATG 5 cut(s) 207, 632, 790, 989, 998
BstFNI CGCG 1 cut(s) 839
BstHHI GCGC 2 cut(s) 198, 1340
BstKTI GATC 3 cut(s) 289, 370, 765
BstMAI GTCTC 5 cut(s) 228, 303, 318, 908, 1178
BstMBI GATC 3 cut(s) 286, 367, 762
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 7 cut(s) 433, 566, 575, 1315, 1344, 1402, 1411
BstNI CCWGG 1 cut(s) 882
BstNSI RCATGY 2 cut(s) 714, 754
BstSCI CCNGG 1 cut(s) 880
BstSFI CTRYAG 3 cut(s) 21, 188, 1236
BstSLI GKGCMC 1 cut(s) 72
BstUI CGCG 1 cut(s) 839
BstV1I GCAGC 7 cut(s) 35, 38, 44, 553, 565, 1255, 1322
BstV2I GAAGAC 1 cut(s) 1434
BstXI CCANNNNNNTGG 1 cut(s) 1083
Bsu15I ATCGAT 1 cut(s) 691
Bsu36I CCTNAGG 1 cut(s) 480
BsuRI GGCC 2 cut(s) 389, 560
BsuTUI ATCGAT 1 cut(s) 691
BtgI CCRYGG 2 cut(s) 837, 969
BtsCI GGATG 5 cut(s) 207, 632, 790, 989, 998
BtsI GCAGTG 1 cut(s) 1409
BtsIMutI CAGTG 2 cut(s) 79, 1409
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 3 cut(s) 253, 558, 712
CaiI CAGNNNCTG 1 cut(s) 29
CciI TCATGA 1 cut(s) 283
CfoI GCGC 2 cut(s) 198, 1340
Cfr13I GGNCC 1 cut(s) 449
Cfr42I CCGCGG 1 cut(s) 840
ClaI ATCGAT 1 cut(s) 691
CseI GACGC 2 cut(s) 483, 1402
Csp6I GTAC 3 cut(s) 359, 507, 1096
CspCI CAANNNNNGTGG 2 cut(s) 799, 834
CviAII CATG 5 cut(s) 284, 672, 711, 751, 847
CviQI GTAC 3 cut(s) 359, 507, 1096
DdeI CTNAG 7 cut(s) 395, 480, 873, 1054, 1167, 1194, 1377
DpnI GATC 3 cut(s) 288, 369, 764
DpnII GATC 3 cut(s) 286, 367, 762
Eam1104I CTCTTC 2 cut(s) 468, 1168
EarI CTCTTC 2 cut(s) 468, 1168
Ecl136II GAGCTC 1 cut(s) 596
Eco130I CCWWGG 4 cut(s) 61, 307, 518, 1285
Eco24I GRGCYC 1 cut(s) 598
Eco31I GGTCTC 2 cut(s) 318, 908
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 51
Eco81I CCTNAGG 1 cut(s) 480
Eco88I CYCGRG 3 cut(s) 458, 678, 1421
EcoICRI GAGCTC 1 cut(s) 596
EcoRII CCWGG 1 cut(s) 880
EcoT14I CCWWGG 4 cut(s) 61, 307, 518, 1285
EcoT38I GRGCYC 1 cut(s) 598
ErhI CCWWGG 4 cut(s) 61, 307, 518, 1285
FaeI CATG 5 cut(s) 287, 675, 714, 754, 850
FalI AAGNNNNNCTT 2 cut(s) 1373, 1405
FaqI GGGAC 3 cut(s) 169, 481, 1123
FatI CATG 5 cut(s) 283, 671, 710, 750, 846
FauNDI CATATG 1 cut(s) 165
FbaI TGATCA 3 cut(s) 286, 367, 762
Fnu4HI GCNGC 9 cut(s) 24, 27, 58, 554, 567, 837, 840, 1269, 1336
FokI GGATG 5 cut(s) 194, 639, 777, 976, 985
FriOI GRGCYC 1 cut(s) 598
Fsp4HI GCNGC 9 cut(s) 24, 27, 58, 554, 567, 837, 840, 1269, 1336
FspBI CTAG 2 cut(s) 579, 980
FspI TGCGCA 1 cut(s) 1339
GlaI GCGC 2 cut(s) 197, 1339
GluI GCNGC 9 cut(s) 24, 27, 58, 554, 567, 837, 840, 1269, 1336
GsuI CTGGAG 1 cut(s) 1385
HaeIII GGCC 2 cut(s) 389, 560
HapII CCGG 1 cut(s) 858
HgaI GACGC 2 cut(s) 483, 1402
HhaI GCGC 2 cut(s) 198, 1340
Hin1II CATG 5 cut(s) 287, 675, 714, 754, 850
Hin6I GCGC 2 cut(s) 196, 1338
HinP1I GCGC 2 cut(s) 196, 1338
HindIII AAGCTT 1 cut(s) 546
HinfI GANTC 5 cut(s) 47, 221, 1058, 1373, 1424
HpaII CCGG 1 cut(s) 858
HphI GGTGA 3 cut(s) 365, 757, 1145
Hpy188I TCNGA 6 cut(s) 128, 472, 1057, 1210, 1372, 1380
Hpy188III TCNNGA 8 cut(s) 284, 401, 458, 760, 918, 980, 1108, 1421
HpyAV CCTTC 7 cut(s) 34, 166, 229, 379, 770, 1014, 1375
HpyCH4III ACNGT 6 cut(s) 74, 192, 700, 973, 1116, 1358
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 7 cut(s) 23, 421, 569, 696, 704, 733, 1271
HpyF10VI GCNNNNNNNGC 7 cut(s) 433, 566, 575, 1315, 1344, 1402, 1411
HpyF3I CTNAG 7 cut(s) 395, 480, 873, 1054, 1167, 1194, 1377
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 5 cut(s) 287, 675, 714, 754, 850
HspAI GCGC 2 cut(s) 196, 1338
KpnI GGTACC 1 cut(s) 1099
Ksp22I TGATCA 3 cut(s) 286, 367, 762
KspI CCGCGG 1 cut(s) 840
Kzo9I GATC 3 cut(s) 286, 367, 762
LguI GCTCTTC 1 cut(s) 468
Lsp1109I GCAGC 7 cut(s) 35, 38, 44, 553, 565, 1255, 1322
LweI GCATC 3 cut(s) 617, 683, 1349
MaeI CTAG 2 cut(s) 579, 980
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 973
MalI GATC 3 cut(s) 288, 369, 764
MbiI CCGCTC 1 cut(s) 447
MboI GATC 3 cut(s) 286, 367, 762
MhlI GDGCHC 3 cut(s) 72, 598, 1436
MlyI GAGTC 2 cut(s) 230, 1433
MmeI TCCRAC 3 cut(s) 1086, 1168, 1237
MseI TTAA 2 cut(s) 99, 1178
MslI CAYNNNNRTG 1 cut(s) 709
MspA1I CMGCKG 2 cut(s) 29, 839
MspI CCGG 1 cut(s) 858
MspR9I CCNGG 1 cut(s) 882
Mva1269I GAATGC 1 cut(s) 1330
MvaI CCWGG 1 cut(s) 882
MvnI CGCG 1 cut(s) 839
MwoI GCNNNNNNNGC 7 cut(s) 433, 566, 575, 1315, 1344, 1402, 1411
NdeI CATATG 1 cut(s) 165
NdeII GATC 3 cut(s) 286, 367, 762
NlaIII CATG 5 cut(s) 287, 675, 714, 754, 850
NlaIV GGNNCC 3 cut(s) 781, 892, 1097
NsbI TGCGCA 1 cut(s) 1339
NspI RCATGY 2 cut(s) 714, 754
PaeI GCATGC 1 cut(s) 714
PagI TCATGA 1 cut(s) 283
PaqCI CACCTGC 1 cut(s) 718
PciI ACATGT 1 cut(s) 750
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 1 cut(s) 1330
PfeI GAWTC 3 cut(s) 47, 1058, 1373
PflMI CCANNNNNTGG 1 cut(s) 887
PkrI GCNGC 9 cut(s) 25, 28, 59, 555, 568, 838, 841, 1270, 1337
PleI GAGTC 2 cut(s) 229, 1432
PpsI GAGTC 2 cut(s) 229, 1432
PscI ACATGT 1 cut(s) 750
Psp124BI GAGCTC 1 cut(s) 598
Psp6I CCWGG 1 cut(s) 880
PspGI CCWGG 1 cut(s) 880
PspN4I GGNNCC 3 cut(s) 781, 892, 1097
PspPI GGNCC 1 cut(s) 449
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 3 cut(s) 360, 508, 1097
RsaNI GTAC 3 cut(s) 359, 507, 1096
RseI CAYNNNNRTG 1 cut(s) 709
SacI GAGCTC 1 cut(s) 598
SacII CCGCGG 1 cut(s) 840
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 2 cut(s) 99, 1178
SatI GCNGC 9 cut(s) 24, 27, 58, 554, 567, 837, 840, 1269, 1336
Sau3AI GATC 3 cut(s) 286, 367, 762
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 2 cut(s) 230, 1433
ScrFI CCNGG 1 cut(s) 882
SduI GDGCHC 3 cut(s) 72, 598, 1436
SfaNI GCATC 3 cut(s) 617, 683, 1349
SfcI CTRYAG 3 cut(s) 21, 188, 1236
Sfr303I CCGCGG 1 cut(s) 840
SgrBI CCGCGG 1 cut(s) 840
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 709
SphI GCATGC 1 cut(s) 714
SsiI CCGC 4 cut(s) 447, 837, 839, 1021
SspI AATATT 1 cut(s) 337
SspMI CTAG 2 cut(s) 579, 980
SstI GAGCTC 1 cut(s) 598
StyD4I CCNGG 1 cut(s) 880
StyI CCWWGG 4 cut(s) 61, 307, 518, 1285
TaaI ACNGT 6 cut(s) 74, 192, 700, 973, 1116, 1358
TaiI ACGT 1 cut(s) 158
TaqI TCGA 5 cut(s) 204, 273, 598, 691, 866
TaqII GACCGA 1 cut(s) 446
TatI WGTACW 2 cut(s) 358, 506
TauI GCSGC 2 cut(s) 839, 842
TfiI GAWTC 3 cut(s) 47, 1058, 1373
Tru1I TTAA 2 cut(s) 99, 1178
Tru9I TTAA 2 cut(s) 99, 1178
TscAI CASTG 2 cut(s) 79, 1416
TseI GCWGC 7 cut(s) 23, 26, 57, 553, 566, 1268, 1335
TspDTI ATGAA 7 cut(s) 105, 131, 185, 558, 632, 996, 1236
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 2 cut(s) 79, 1416
Van91I CCANNNNNTGG 1 cut(s) 887
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 3 cut(s) 944, 1103, 1216
XbaI TCTAGA 1 cut(s) 979
XceI RCATGY 2 cut(s) 714, 754
XspI CTAG 2 cut(s) 579, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.