RLG00000021823
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011709
Physical Location & Seq
Reverse (-)
78592964 .. 78594859
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021823

Sequence Viewer

Length: 1467 bp
ATGCCAAACTCTAATTCAACAGCAGCAGCTGAAGAAACGAAGGTTGAATCCTTTGGCTACCAAGAAGGGCACAGTGAGGAGAAGAATTATGAACTTGTTAATGCCAAAGCCGAAATGAATGAGGTCAGAGAAGAAAATGCGAGACTGAAAAAGACGTTAGAGCATATGATGAAGGATTACCAGTCTCTACAGTTGCGCTTTTTTGACATCCTCAAACAAGAGTCTCATTCCAAGAAGGCTACAATGAGTGCCAGCACCTCTCATATCGAAATCGAAGAAGCTCATGATCAGCTCTTGTCTCTTTGCCTTGGAAGAAGTCCAAGAGACCCTAAAAATATTAGTGATGAGAGCAAAATTATACAAGTTGATCATCACCAAGACTTGAAGGCCAACCTTACTCTTGAATTAGGCTCTAAATTGCAGTTGCCGACGGAGCTTGTATTGAGCGGTCCAATTCCCGAGAATAGTTCCGAAGAGCCCAAGGAAGCAGAAGCGTCCCCATCAAGTACTAAAACCCCAAAGACAATGAGAAACGAAGATGATGAAGCTTCGCAGCAGGCCAATGCTGCAAAAAGAGCTAGGGTTTCCGTGAGAGCTCGATGTGATACCCCCACGATGAATGATGGATGCCAATGGAGAAAATATGGACAGAAGATTGCGAAAGGAAATCCATGCCCCCGAGCATACTATCGATGCACGGTTGCACCAGCATGCCCTGTTAGAAAGCAGGTGCAAAGATGTATTGATGACATGTCTATCTTGATCACCACCTACGAAGGAACCCACAACCATCCACTTCCAATGACAGCCACTCCCATGGCTTCCACCACCTCTGCCGCGGCTTCCATGTTATTGTCCGGCTCTTCGACATCTCAGCTTCCAGGTCTTGGTTCCAGTTTTGGTCTCTTCGATAATTCCAGACGACAACAGCTCTACTTGCCTAAATTTTCCTCATCTCCTTTGCTACCCACAGTTACTCTAGACCTCACATCTTCTCCATCCCCTTCATCAACCCAATACTGCACATTTCCTCCAAGTGTTAGCTTTTGTTCCTCAGAATCCAACATCAATTTACCGACCAGTTGGGGCAATGGGTACCCTAAATTCGGGACACCGTTTGACAGAAAGAACCAAGAGTGTTTCCAACCCAATCACCAAGCCTCTTCTCAGGTGTCCTTAACAGAGACATTGACCAAGGCAATCACATCAGACCCAAATTTCAAGTCGGTAATTGCTGTAGCGCTTTCATCTATGGTTGGAGGTGGTGCTGCAAGTTATGGAAGCCAAGGAATAGGGGAAAGGCAACACTTGACACAGAATGCGAAAGGCTGCGCATCAAGCATTTTCAACAGTTTGTCATCTTCCGATTCTCAGAAGGGAAACTTGACGCTTTTGCACCCTCCACCGCCATTTCCCCAGTCCAAGAGCACTTCGTCTTCCCCTGCCATCAGGGATAAAAGTCTCTAA

Protein Analysis

489

Amino Acids

53.16

Weight (kDa)

7.16

Isoelectric Point (pI)

58.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 207 - 265 1.8e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011709)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15130 AT5G15130
fragaria_vesca FvH4_6g49140 FvH4_6g49140
malus_domestica MD09G1048200.v1.1 MD09G1048300.v1.1 MD17G1048400.v1.1
prunus_persica Prupe.3G270800_v2.0.a1
pyrus_communis pycom111g03910
rosa_chinensis RchiOBHm_Chr2g0169011
rosa_laevigata RLG00000021823
rosa_multiflora Rmu_ssc0000393.1_g000021
rosa_roxburghii Rroxscaffold_2G00082540
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG607700 Rh2BG620200 Rh2CG589200 Rh2DG631000
rosa_wichuraiana Rw2G050390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 718
Acc16I TGCGCA 1 cut(s) 1333
Acc36I ACCTGC 1 cut(s) 718
Acc65I GGTACC 1 cut(s) 1095
AccB1I GGYRCC 1 cut(s) 1095
AccB7I CCANNNNNTGG 1 cut(s) 887
AccBSI CCGCTC 1 cut(s) 447
AccII CGCG 1 cut(s) 839
AciI CCGC 4 cut(s) 447, 837, 839, 1406
AcsI RAATTY 3 cut(s) 944, 1103, 1216
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 2 cut(s) 508, 1097
AfeI AGCGCT 1 cut(s) 1242
AfiI CCNNNNNNNGG 2 cut(s) 887, 1106
AflIII ACRYGT 1 cut(s) 750
AgsI TTSAA 6 cut(s) 18, 47, 385, 404, 1222, 1348
AjnI CCWGG 1 cut(s) 880
AjuI GAANNNNNNNTTGG 1 cut(s) 30
Alw21I GWGCWC 2 cut(s) 598, 1430
Alw26I GTCTC 7 cut(s) 136, 189, 228, 303, 318, 908, 1178
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 2 cut(s) 458, 678
Aor51HI AGCGCT 1 cut(s) 1242
AoxI GGCC 2 cut(s) 387, 558
ApeKI GCWGC 6 cut(s) 23, 26, 553, 566, 1268, 1329
ApoI RAATTY 3 cut(s) 944, 1103, 1216
ArsI GACNNNNNNTTYG 2 cut(s) 1208, 1240
Asp718I GGTACC 1 cut(s) 1095
AspLEI GCGC 3 cut(s) 198, 1243, 1334
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 3 cut(s) 365, 757, 1145
AvaI CYCGRG 2 cut(s) 458, 678
AvaII GGWCC 1 cut(s) 449
BaeGI GKGCMC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 1095
BanII GRGCYC 2 cut(s) 480, 598
BbsI GAAGAC 1 cut(s) 1428
Bbv12I GWGCWC 2 cut(s) 598, 1430
BbvI GCAGC 6 cut(s) 35, 38, 553, 565, 1255, 1316
BccI CCATC 5 cut(s) 508, 617, 798, 1006, 1454
BciT130I CCWGG 1 cut(s) 882
BclI TGATCA 3 cut(s) 286, 367, 762
BcoDI GTCTC 7 cut(s) 136, 189, 228, 303, 318, 908, 1178
BfaI CTAG 2 cut(s) 579, 980
BfmI CTRYAG 2 cut(s) 188, 1236
BfoI RGCGCY 1 cut(s) 1244
BfuAI ACCTGC 1 cut(s) 718
BisI GCNGC 8 cut(s) 24, 27, 554, 567, 837, 840, 1269, 1330
BlsI GCNGC 8 cut(s) 25, 28, 555, 568, 838, 841, 1270, 1331
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 882
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 2 cut(s) 458, 678
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 3 cut(s) 781, 892, 1097
BmrFI CCNGG 1 cut(s) 882
BmrI ACTGGG 1 cut(s) 1411
BmsI GCATC 3 cut(s) 617, 683, 1343
BmuI ACTGGG 1 cut(s) 1411
BpiI GAAGAC 1 cut(s) 1428
Bsa29I ATCGAT 1 cut(s) 691
BsaI GGTCTC 2 cut(s) 318, 908
BsaJI CCNNGG 6 cut(s) 307, 480, 816, 837, 1194, 1285
BsaXI ACNNNNNCTCC 6 cut(s) 796, 826, 979, 1009, 1015, 1045
Bsc4I CCNNNNNNNGG 2 cut(s) 887, 1106
Bse1I ACTGG 4 cut(s) 181, 894, 1080, 1417
Bse3DI GCAATG 1 cut(s) 1096
BseBI CCWGG 1 cut(s) 882
BseCI ATCGAT 1 cut(s) 691
BseDI CCNNGG 6 cut(s) 307, 480, 816, 837, 1194, 1285
BseGI GGATG 4 cut(s) 207, 632, 790, 998
BseLI CCNNNNNNNGG 2 cut(s) 887, 1106
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 4 cut(s) 887, 1068, 1181, 1385
BseNI ACTGG 4 cut(s) 181, 894, 1080, 1417
BseRI GAGGAG 1 cut(s) 92
BseSI GKGCMC 1 cut(s) 72
BseXI GCAGC 6 cut(s) 35, 38, 553, 565, 1255, 1316
BsgI GTGCAG 1 cut(s) 1006
Bsh1236I CGCG 1 cut(s) 839
BshFI GGCC 2 cut(s) 389, 560
BshNI GGYRCC 1 cut(s) 1095
BshVI ATCGAT 1 cut(s) 691
BsiHKAI GWGCWC 2 cut(s) 598, 1430
BsiHKCI CYCGRG 2 cut(s) 458, 678
BsiSI CCGG 1 cut(s) 858
BslFI GGGAC 2 cut(s) 481, 1123
BslI CCNNNNNNNGG 2 cut(s) 887, 1106
BsmAI GTCTC 7 cut(s) 136, 189, 228, 303, 318, 908, 1178
BsmFI GGGAC 2 cut(s) 481, 1123
BsmI GAATGC 1 cut(s) 1324
BsnI GGCC 2 cut(s) 389, 560
Bso31I GGTCTC 2 cut(s) 318, 908
BsoBI CYCGRG 2 cut(s) 458, 678
Bsp1286I GDGCHC 4 cut(s) 72, 480, 598, 1430
Bsp143I GATC 3 cut(s) 286, 367, 762
Bsp19I CCATGG 1 cut(s) 816
BspACI CCGC 4 cut(s) 447, 837, 839, 1406
BspANI GGCC 2 cut(s) 389, 560
BspCNI CTCAG 4 cut(s) 886, 1067, 1180, 1384
BspDI ATCGAT 1 cut(s) 691
BspFNI CGCG 1 cut(s) 839
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 3 cut(s) 781, 892, 1097
BspMI ACCTGC 1 cut(s) 718
BspQI GCTCTTC 2 cut(s) 468, 868
BspT107I GGYRCC 1 cut(s) 1095
BspTNI GGTCTC 2 cut(s) 318, 908
BsrBI CCGCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 1096
BsrI ACTGG 4 cut(s) 181, 894, 1080, 1417
BssECI CCNNGG 6 cut(s) 307, 480, 816, 837, 1194, 1285
BssMI GATC 3 cut(s) 286, 367, 762
BssT1I CCWWGG 5 cut(s) 307, 480, 816, 1194, 1285
Bst2UI CCWGG 1 cut(s) 882
Bst4CI ACNGT 6 cut(s) 74, 192, 700, 973, 1116, 1352
Bst6I CTCTTC 4 cut(s) 468, 868, 911, 1168
BstC8I GCNNGC 3 cut(s) 253, 558, 712
BstDEI CTNAG 4 cut(s) 873, 1054, 1167, 1371
BstDSI CCRYGG 2 cut(s) 816, 837
BstF5I GGATG 4 cut(s) 207, 632, 790, 998
BstFNI CGCG 1 cut(s) 839
BstH2I RGCGCY 1 cut(s) 1244
BstHHI GCGC 3 cut(s) 198, 1243, 1334
BstKTI GATC 3 cut(s) 289, 370, 765
BstMAI GTCTC 7 cut(s) 136, 189, 228, 303, 318, 908, 1178
BstMBI GATC 3 cut(s) 286, 367, 762
BstMWI GCNNNNNNNGC 5 cut(s) 433, 566, 575, 937, 1338
BstNI CCWGG 1 cut(s) 882
BstNSI RCATGY 2 cut(s) 714, 754
BstSCI CCNGG 1 cut(s) 880
BstSFI CTRYAG 2 cut(s) 188, 1236
BstSLI GKGCMC 1 cut(s) 72
BstUI CGCG 1 cut(s) 839
BstV1I GCAGC 6 cut(s) 35, 38, 553, 565, 1255, 1316
BstV2I GAAGAC 1 cut(s) 1428
BstXI CCANNNNNNTGG 1 cut(s) 817
Bsu15I ATCGAT 1 cut(s) 691
BsuRI GGCC 2 cut(s) 389, 560
BsuTUI ATCGAT 1 cut(s) 691
BtgI CCRYGG 2 cut(s) 816, 837
BtsCI GGATG 4 cut(s) 207, 632, 790, 998
BtsIMutI CAGTG 1 cut(s) 79
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 3 cut(s) 253, 558, 712
CaiI CAGNNNCTG 1 cut(s) 29
CciI TCATGA 1 cut(s) 283
CfoI GCGC 3 cut(s) 198, 1243, 1334
Cfr13I GGNCC 1 cut(s) 449
Cfr42I CCGCGG 1 cut(s) 840
ClaI ATCGAT 1 cut(s) 691
CseI GACGC 2 cut(s) 483, 1396
Csp6I GTAC 2 cut(s) 507, 1096
CviAII CATG 6 cut(s) 284, 672, 711, 751, 817, 847
CviQI GTAC 2 cut(s) 507, 1096
DdeI CTNAG 4 cut(s) 873, 1054, 1167, 1371
DpnI GATC 3 cut(s) 288, 369, 764
DpnII GATC 3 cut(s) 286, 367, 762
Eam1104I CTCTTC 4 cut(s) 468, 868, 911, 1168
EarI CTCTTC 4 cut(s) 468, 868, 911, 1168
Ecl136II GAGCTC 1 cut(s) 596
Eco130I CCWWGG 5 cut(s) 307, 480, 816, 1194, 1285
Eco24I GRGCYC 2 cut(s) 480, 598
Eco31I GGTCTC 2 cut(s) 318, 908
Eco47I GGWCC 1 cut(s) 449
Eco47III AGCGCT 1 cut(s) 1242
Eco53kI GAGCTC 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 51
Eco88I CYCGRG 2 cut(s) 458, 678
EcoICRI GAGCTC 1 cut(s) 596
EcoRII CCWGG 1 cut(s) 880
EcoT14I CCWWGG 5 cut(s) 307, 480, 816, 1194, 1285
EcoT38I GRGCYC 2 cut(s) 480, 598
ErhI CCWWGG 5 cut(s) 307, 480, 816, 1194, 1285
FaeI CATG 6 cut(s) 287, 675, 714, 754, 820, 850
FalI AAGNNNNNCTT 2 cut(s) 1367, 1399
FaqI GGGAC 2 cut(s) 481, 1123
FatI CATG 6 cut(s) 283, 671, 710, 750, 816, 846
FauNDI CATATG 1 cut(s) 165
FbaI TGATCA 3 cut(s) 286, 367, 762
Fnu4HI GCNGC 8 cut(s) 24, 27, 554, 567, 837, 840, 1269, 1330
FokI GGATG 4 cut(s) 194, 639, 777, 985
FriOI GRGCYC 2 cut(s) 480, 598
Fsp4HI GCNGC 8 cut(s) 24, 27, 554, 567, 837, 840, 1269, 1330
FspBI CTAG 2 cut(s) 579, 980
FspI TGCGCA 1 cut(s) 1333
GlaI GCGC 3 cut(s) 197, 1242, 1333
GluI GCNGC 8 cut(s) 24, 27, 554, 567, 837, 840, 1269, 1330
HaeII RGCGCY 1 cut(s) 1244
HaeIII GGCC 2 cut(s) 389, 560
HapII CCGG 1 cut(s) 858
HgaI GACGC 2 cut(s) 483, 1396
HhaI GCGC 3 cut(s) 198, 1243, 1334
Hin1II CATG 6 cut(s) 287, 675, 714, 754, 820, 850
Hin6I GCGC 3 cut(s) 196, 1241, 1332
HinP1I GCGC 3 cut(s) 196, 1241, 1332
HindIII AAGCTT 1 cut(s) 546
HinfI GANTC 4 cut(s) 47, 221, 1058, 1367
HpaII CCGG 1 cut(s) 858
HphI GGTGA 3 cut(s) 365, 757, 1145
Hpy188I TCNGA 6 cut(s) 128, 472, 1057, 1210, 1366, 1374
Hpy188III TCNNGA 7 cut(s) 284, 401, 458, 760, 918, 980, 1108
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 8 cut(s) 34, 59, 166, 229, 379, 770, 1014, 1369
HpyCH4III ACNGT 6 cut(s) 74, 192, 700, 973, 1116, 1352
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 8 cut(s) 421, 569, 696, 704, 733, 1023, 1271, 1396
HpyF10VI GCNNNNNNNGC 5 cut(s) 433, 566, 575, 937, 1338
HpyF3I CTNAG 4 cut(s) 873, 1054, 1167, 1371
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 6 cut(s) 287, 675, 714, 754, 820, 850
HspAI GCGC 3 cut(s) 196, 1241, 1332
KpnI GGTACC 1 cut(s) 1099
Ksp22I TGATCA 3 cut(s) 286, 367, 762
KspI CCGCGG 1 cut(s) 840
Kzo9I GATC 3 cut(s) 286, 367, 762
LguI GCTCTTC 2 cut(s) 468, 868
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 6 cut(s) 35, 38, 553, 565, 1255, 1316
LweI GCATC 3 cut(s) 617, 683, 1343
MaeI CTAG 2 cut(s) 579, 980
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 973
MalI GATC 3 cut(s) 288, 369, 764
MbiI CCGCTC 1 cut(s) 447
MboI GATC 3 cut(s) 286, 367, 762
MhlI GDGCHC 4 cut(s) 72, 480, 598, 1430
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 3 cut(s) 1086, 1168, 1237
MseI TTAA 2 cut(s) 99, 1178
MslI CAYNNNNRTG 2 cut(s) 709, 815
MspA1I CMGCKG 2 cut(s) 29, 839
MspI CCGG 1 cut(s) 858
MspR9I CCNGG 1 cut(s) 882
Mva1269I GAATGC 1 cut(s) 1324
MvaI CCWGG 1 cut(s) 882
MvnI CGCG 1 cut(s) 839
MwoI GCNNNNNNNGC 5 cut(s) 433, 566, 575, 937, 1338
NcoI CCATGG 1 cut(s) 816
NdeI CATATG 1 cut(s) 165
NdeII GATC 3 cut(s) 286, 367, 762
NlaIII CATG 6 cut(s) 287, 675, 714, 754, 820, 850
NlaIV GGNNCC 3 cut(s) 781, 892, 1097
NsbI TGCGCA 1 cut(s) 1333
NspI RCATGY 2 cut(s) 714, 754
PaeI GCATGC 1 cut(s) 714
PagI TCATGA 1 cut(s) 283
PaqCI CACCTGC 1 cut(s) 718
PciI ACATGT 1 cut(s) 750
PciSI GCTCTTC 2 cut(s) 468, 868
PctI GAATGC 1 cut(s) 1324
PfeI GAWTC 3 cut(s) 47, 1058, 1367
PflMI CCANNNNNTGG 1 cut(s) 887
PkrI GCNGC 8 cut(s) 25, 28, 555, 568, 838, 841, 1270, 1331
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PscI ACATGT 1 cut(s) 750
Psp124BI GAGCTC 1 cut(s) 598
Psp6I CCWGG 1 cut(s) 880
PspGI CCWGG 1 cut(s) 880
PspN4I GGNNCC 3 cut(s) 781, 892, 1097
PspPI GGNCC 1 cut(s) 449
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 2 cut(s) 508, 1097
RsaNI GTAC 2 cut(s) 507, 1096
RseI CAYNNNNRTG 2 cut(s) 709, 815
SacI GAGCTC 1 cut(s) 598
SacII CCGCGG 1 cut(s) 840
SapI GCTCTTC 2 cut(s) 468, 868
SaqAI TTAA 2 cut(s) 99, 1178
SatI GCNGC 8 cut(s) 24, 27, 554, 567, 837, 840, 1269, 1330
Sau3AI GATC 3 cut(s) 286, 367, 762
Sau96I GGNCC 1 cut(s) 449
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 882
SduI GDGCHC 4 cut(s) 72, 480, 598, 1430
SfaNI GCATC 3 cut(s) 617, 683, 1343
SfcI CTRYAG 2 cut(s) 188, 1236
Sfr303I CCGCGG 1 cut(s) 840
SgrBI CCGCGG 1 cut(s) 840
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 2 cut(s) 709, 815
SphI GCATGC 1 cut(s) 714
SsiI CCGC 4 cut(s) 447, 837, 839, 1406
SspI AATATT 1 cut(s) 337
SspMI CTAG 2 cut(s) 579, 980
SstI GAGCTC 1 cut(s) 598
StyD4I CCNGG 1 cut(s) 880
StyI CCWWGG 5 cut(s) 307, 480, 816, 1194, 1285
TaaI ACNGT 6 cut(s) 74, 192, 700, 973, 1116, 1352
TaiI ACGT 1 cut(s) 158
TaqI TCGA 6 cut(s) 267, 273, 598, 691, 866, 909
TatI WGTACW 1 cut(s) 506
TauI GCSGC 2 cut(s) 839, 842
TfiI GAWTC 3 cut(s) 47, 1058, 1367
Tru1I TTAA 2 cut(s) 99, 1178
Tru9I TTAA 2 cut(s) 99, 1178
TscAI CASTG 1 cut(s) 79
TseI GCWGC 6 cut(s) 23, 26, 553, 566, 1268, 1329
TspDTI ATGAA 7 cut(s) 105, 131, 185, 558, 632, 996, 1236
TspGWI ACGGA 2 cut(s) 446, 577
TspRI CASTG 1 cut(s) 79
Van91I CCANNNNNTGG 1 cut(s) 887
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 3 cut(s) 944, 1103, 1216
XbaI TCTAGA 1 cut(s) 979
XceI RCATGY 2 cut(s) 714, 754
XspI CTAG 2 cut(s) 579, 980
ZrmI AGTACT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.