MD17G1048400.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011709
Physical Location & Seq
Forward (+)
3528939 .. 3531555
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1048400.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGAGAATGCCAAACTCCAACAGCAGATCATCTGCAGAAAAGAAAGTTGATCAATCTGTTGGATATCAGGAGGTTCACAGGCGGGTTTTAAGAAAGGGGAGTGAACTGGATGATGCCAAAGCTGAAATGGGAGAGGTGCGAGAAGAAAACGCTAGGTTAAAAATGACGTTAAATCATATACAGAAGGATTACCAGTCTCTCCAGCTGCGGTTTTTCGACATCCTCAGACAAGAGGCTTCCAAAAACGCTGTAAATGTTGCCGGTGATCGAATAATCGAAGAACCAGAGCAGCTCTTGTCTCTTTGTCTTGGAAGAAGTCCAAGGGAGCCTACAAGTGATCGTGAAACTACTACCAGCAGCAACTTCACAAAACTAGTACAGGTTGATGATGGTGAAGATAACTTGAAGGCCAATCTTACTCTTGGATTAGGTTCTAATGAGACTAGTTTGGAGGAACCTAATAAGGATCGTGCAGAAGCTGGAAGTGGAGACGCAACGTCTCCATTGAGTAAGACGCCGAAGACGATGAGAAATGAAGACGGTGAACCTCCAACGCAGGTCAATGCAAAACGAGCTAGGGTTTCTGTGAGAACTAGATGTGATACCCCAACGATGAATGATGGTTGCCAATGGAGAAAATATGGACAGAAGATTTCAAAAGGAAATCCATGCCCACGAGCGTACTATCGGTGCACAGTTGCACCAGCATGCCCTGTGAGAAAGCAGGTGCAAAGATGCTATGAGGATATGTCTATCTTAATCACCACCTATGAAGGAACTCACAACCACCCGCTTCCATTCACAGCCACTTACTTGGCTTCCACCACCTCTGCCGCGGCTTCCATGCTATTGTCCGGCTCTTGTACATCTCACCCGGGTTTCAGTACCACATCCACCCTACTCAATGGATCAGAATTTAGTGCCTTCGATAACACAAGAACACAACTCTACATGCCCAAATCTTCCCAACCTTTGCTCCCCACAATCACTCTCGACCTCACTGCCTCTCCACCCTCTTCTAGCACATTTCCTTCAAGTCTAAGCTTTTGTTCCCCATCAGATTCCAACATATTCCCCACAAGTTGGGACAATGGATACCTCAAACATGGCTTGCACCAGTTCGACAAAGCTCAAGTTGGATCCTTCAATCTTGGAAAGCCATCGCAAGAGTATTTCCACCAAAATTATGCAGAAATGATCCACCAAGCTTCTTCCCAGGTGTCCTTGACAGAATCATTAACCAAGGCAATCACGTCCAACCCGAATTTCAAGTCGGTAATTGCATTAGCACTTTCATCTATGGTCGGAAGTGGAGGTGGTGCCACTCATAGGAACCAAGGCGAGAGTGGCGAAAGAATCAACACTTGA

Protein Analysis

456

Amino Acids

49.96

Weight (kDa)

7.59

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 206 - 264 1.9e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011709)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15130 AT5G15130
fragaria_vesca FvH4_6g49140 FvH4_6g49140
malus_domestica MD09G1048200.v1.1 MD09G1048300.v1.1 MD17G1048400.v1.1
prunus_persica Prupe.3G270800_v2.0.a1
pyrus_communis pycom111g03910
rosa_chinensis RchiOBHm_Chr2g0169011
rosa_laevigata RLG00000021823
rosa_multiflora Rmu_ssc0000393.1_g000021
rosa_roxburghii Rroxscaffold_2G00082540
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG607700 Rh2BG620200 Rh2CG589200 Rh2DG631000
rosa_wichuraiana Rw2G050390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 715
Acc36I ACCTGC 2 cut(s) 547, 715
AccB1I GGYRCC 1 cut(s) 1319
AccB7I CCANNNNNTGG 1 cut(s) 1083
AccII CGCG 1 cut(s) 836
AciI CCGC 5 cut(s) 82, 208, 791, 834, 836
AclWI GGATC 5 cut(s) 474, 916, 1134, 1147, 1192
AcsI RAATTY 2 cut(s) 914, 1264
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 4 cut(s) 378, 683, 865, 886
AfiI CCNNNNNNNGG 2 cut(s) 1083, 1329
AgsI TTSAA 5 cut(s) 406, 657, 1035, 1147, 1270
AhdI GACNNNNNGTC 1 cut(s) 496
AhlI ACTAGT 2 cut(s) 373, 443
AjiI CACGTC 1 cut(s) 1254
AjnI CCWGG 1 cut(s) 1215
AjuI GAANNNNNNNTTGG 2 cut(s) 1250, 1282
AluBI AGCT 8 cut(s) 122, 205, 292, 479, 575, 1044, 1130, 1208
AluI AGCT 8 cut(s) 122, 205, 292, 479, 575, 1044, 1130, 1208
Alw21I GWGCWC 1 cut(s) 695
Alw26I GTCTC 5 cut(s) 201, 303, 434, 483, 504
Alw44I GTGCAC 1 cut(s) 691
AlwI GGATC 5 cut(s) 474, 916, 1134, 1147, 1192
AlwNI CAGNNNCTG 1 cut(s) 479
Ama87I CYCGRG 1 cut(s) 874
AoxI GGCC 1 cut(s) 408
ApaLI GTGCAC 1 cut(s) 691
ApeKI GCWGC 3 cut(s) 205, 289, 357
ApoI RAATTY 2 cut(s) 914, 1264
ArsI GACNNNNNNTTYG 2 cut(s) 1256, 1288
AsuC2I CCSGG 2 cut(s) 875, 876
AsuHPI GGTGA 5 cut(s) 275, 404, 554, 754, 863
AvaI CYCGRG 1 cut(s) 874
BaeGI GKGCMC 1 cut(s) 695
BamHI GGATCC 1 cut(s) 1139
BanI GGYRCC 1 cut(s) 1319
BauI CACGAG 1 cut(s) 675
BbsI GAAGAC 2 cut(s) 527, 543
Bbv12I GWGCWC 1 cut(s) 695
BbvI GCAGC 3 cut(s) 192, 301, 369
BccI CCATC 4 cut(s) 383, 614, 1063, 1168
BcgI CGANNNNNNTGC 2 cut(s) 983, 1017
BciT130I CCWGG 1 cut(s) 1217
BciVI GTATCC 1 cut(s) 1088
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 2 cut(s) 875, 876
BcoDI GTCTC 5 cut(s) 201, 303, 434, 483, 504
BcuI ACTAGT 2 cut(s) 373, 443
BfaI CTAG 6 cut(s) 153, 374, 444, 576, 594, 1020
BfmI CTRYAG 1 cut(s) 33
BfuAI ACCTGC 2 cut(s) 547, 715
BfuI GTATCC 1 cut(s) 1088
BisI GCNGC 5 cut(s) 206, 290, 358, 834, 837
BlsI GCNGC 5 cut(s) 207, 291, 359, 835, 838
Bme1390I CCNGG 3 cut(s) 875, 876, 1217
BmeRI GACNNNNNGTC 1 cut(s) 496
BmeT110I CYCGRG 1 cut(s) 874
BmgBI CACGTC 1 cut(s) 1254
BmiI GGNNCC 5 cut(s) 327, 456, 1141, 1321, 1334
BmrFI CCNGG 3 cut(s) 875, 876, 1217
BmsI GCATC 2 cut(s) 103, 725
BpiI GAAGAC 2 cut(s) 527, 543
BpmI CTGGAG 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 1116
BpuMI CCSGG 2 cut(s) 875, 876
BsaHI GRCGYC 1 cut(s) 515
BsaJI CCNNGG 6 cut(s) 320, 834, 874, 1215, 1242, 1336
BsaXI ACNNNNNCTCC 4 cut(s) 960, 990, 991, 1021
Bsc4I CCNNNNNNNGG 2 cut(s) 1083, 1329
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 3 cut(s) 111, 193, 1117
BseBI CCWGG 1 cut(s) 1217
BseDI CCNNGG 6 cut(s) 320, 834, 874, 1215, 1242, 1336
BseGI GGATG 3 cut(s) 115, 219, 890
BseLI CCNNNNNNNGG 2 cut(s) 1083, 1329
BseMII CTCAG 1 cut(s) 238
BseNI ACTGG 3 cut(s) 111, 193, 1117
BseSI GKGCMC 1 cut(s) 695
BseXI GCAGC 3 cut(s) 192, 301, 369
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 836
BshFI GGCC 1 cut(s) 410
BshNI GGYRCC 1 cut(s) 1319
BsiHKAI GWGCWC 1 cut(s) 695
BsiHKCI CYCGRG 1 cut(s) 874
BsiSI CCGG 3 cut(s) 261, 855, 875
BslFI GGGAC 1 cut(s) 1100
BslI CCNNNNNNNGG 2 cut(s) 1083, 1329
BsmAI GTCTC 5 cut(s) 201, 303, 434, 483, 504
BsmBI CGTCTC 2 cut(s) 483, 504
BsmFI GGGAC 1 cut(s) 1100
BsmI GAATGC 1 cut(s) 12
BsnI GGCC 1 cut(s) 410
BsoBI CYCGRG 1 cut(s) 874
Bsp1286I GDGCHC 1 cut(s) 695
Bsp1407I TGTACA 1 cut(s) 863
Bsp143I GATC 8 cut(s) 26, 49, 265, 337, 466, 908, 1139, 1197
BspACI CCGC 5 cut(s) 82, 208, 791, 834, 836
BspANI GGCC 1 cut(s) 410
BspCNI CTCAG 1 cut(s) 237
BspFNI CGCG 1 cut(s) 836
BspLI GGNNCC 5 cut(s) 327, 456, 1141, 1321, 1334
BspMAI CTGCAG 1 cut(s) 37
BspMI ACCTGC 2 cut(s) 547, 715
BspPI GGATC 5 cut(s) 474, 916, 1134, 1147, 1192
BspT107I GGYRCC 1 cut(s) 1319
BsrFI RCCGGY 1 cut(s) 260
BsrGI TGTACA 1 cut(s) 863
BsrI ACTGG 3 cut(s) 111, 193, 1117
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 6 cut(s) 320, 834, 874, 1215, 1242, 1336
BssMI GATC 8 cut(s) 26, 49, 265, 337, 466, 908, 1139, 1197
BssNI GRCGYC 1 cut(s) 515
BssSI CACGAG 1 cut(s) 675
BssT1I CCWWGG 3 cut(s) 320, 1242, 1336
Bst2BI CACGAG 1 cut(s) 675
Bst2UI CCWGG 1 cut(s) 1217
Bst4CI ACNGT 2 cut(s) 542, 697
Bst6I CTCTTC 1 cut(s) 1021
BstACI GRCGYC 1 cut(s) 515
BstAUI TGTACA 1 cut(s) 863
BstC8I GCNNGC 2 cut(s) 709, 1112
BstDEI CTNAG 2 cut(s) 224, 1040
BstDSI CCRYGG 1 cut(s) 834
BstF5I GGATG 3 cut(s) 115, 219, 890
BstFNI CGCG 1 cut(s) 836
BstKTI GATC 8 cut(s) 29, 52, 268, 340, 469, 911, 1142, 1200
BstMAI GTCTC 5 cut(s) 201, 303, 434, 483, 504
BstMBI GATC 8 cut(s) 26, 49, 265, 337, 466, 908, 1139, 1197
BstMWI GCNNNNNNNGC 2 cut(s) 572, 1347
BstNI CCWGG 1 cut(s) 1217
BstNSI RCATGY 2 cut(s) 711, 955
BstSCI CCNGG 3 cut(s) 873, 874, 1215
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 695
BstUI CGCG 1 cut(s) 836
BstV1I GCAGC 3 cut(s) 192, 301, 369
BstV2I GAAGAC 2 cut(s) 527, 543
BstX2I RGATCY 1 cut(s) 1139
BstXI CCANNNNNNTGG 1 cut(s) 814
BstYI RGATCY 1 cut(s) 1139
BsuI GTATCC 1 cut(s) 1088
BsuRI GGCC 1 cut(s) 410
BtgI CCRYGG 1 cut(s) 834
BtgZI GCGATG 1 cut(s) 1146
BtrI CACGTC 1 cut(s) 1254
BtsCI GGATG 3 cut(s) 115, 219, 890
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 1 cut(s) 999
BveI ACCTGC 2 cut(s) 547, 715
Cac8I GCNNGC 2 cut(s) 709, 1112
CaiI CAGNNNCTG 1 cut(s) 479
Cfr10I RCCGGY 1 cut(s) 260
Cfr42I CCGCGG 1 cut(s) 837
Cfr9I CCCGGG 1 cut(s) 874
CseI GACGC 2 cut(s) 500, 523
Csp6I GTAC 4 cut(s) 377, 682, 864, 885
CviAII CATG 5 cut(s) 669, 708, 844, 952, 1106
CviQI GTAC 4 cut(s) 377, 682, 864, 885
DdeI CTNAG 2 cut(s) 224, 1040
DpnI GATC 8 cut(s) 28, 51, 267, 339, 468, 910, 1141, 1199
DpnII GATC 8 cut(s) 26, 49, 265, 337, 466, 908, 1139, 1197
DriI GACNNNNNGTC 1 cut(s) 496
Eam1104I CTCTTC 1 cut(s) 1021
Eam1105I GACNNNNNGTC 1 cut(s) 496
EarI CTCTTC 1 cut(s) 1021
Eco130I CCWWGG 3 cut(s) 320, 1242, 1336
Eco32I GATATC 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 874
EcoRII CCWGG 1 cut(s) 1215
EcoRV GATATC 1 cut(s) 65
EcoT14I CCWWGG 3 cut(s) 320, 1242, 1336
ErhI CCWWGG 3 cut(s) 320, 1242, 1336
Esp3I CGTCTC 2 cut(s) 483, 504
FaeI CATG 5 cut(s) 672, 711, 847, 955, 1109
FaqI GGGAC 1 cut(s) 1100
FatI CATG 5 cut(s) 668, 707, 843, 951, 1105
FauI CCCGC 2 cut(s) 75, 798
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 5 cut(s) 206, 290, 358, 834, 837
FokI GGATG 3 cut(s) 122, 206, 877
Fsp4HI GCNGC 5 cut(s) 206, 290, 358, 834, 837
FspBI CTAG 6 cut(s) 153, 374, 444, 576, 594, 1020
GluI GCNGC 5 cut(s) 206, 290, 358, 834, 837
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 1 cut(s) 410
HapII CCGG 3 cut(s) 261, 855, 875
HgaI GACGC 2 cut(s) 500, 523
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 5 cut(s) 672, 711, 847, 955, 1109
HindIII AAGCTT 2 cut(s) 1042, 1206
HinfI GANTC 3 cut(s) 1061, 1232, 1356
HpaII CCGG 3 cut(s) 261, 855, 875
HphI GGTGA 5 cut(s) 275, 404, 554, 754, 863
Hpy166II GTNNAC 4 cut(s) 76, 104, 545, 693
Hpy188I TCNGA 4 cut(s) 227, 913, 1060, 1307
Hpy188III TCNNGA 3 cut(s) 68, 341, 992
Hpy8I GTNNAC 4 cut(s) 76, 104, 545, 693
HpyAV CCTTC 6 cut(s) 178, 400, 767, 934, 1041, 1153
HpyCH4III ACNGT 2 cut(s) 542, 697
HpyCH4IV ACGT 3 cut(s) 167, 497, 1253
HpyCH4V TGCA 9 cut(s) 35, 473, 566, 693, 701, 730, 1114, 1190, 1283
HpyF10VI GCNNNNNNNGC 2 cut(s) 572, 1347
HpyF3I CTNAG 2 cut(s) 224, 1040
HpySE526I ACGT 3 cut(s) 167, 497, 1253
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 5 cut(s) 672, 711, 847, 955, 1109
Ksp22I TGATCA 1 cut(s) 49
KspI CCGCGG 1 cut(s) 837
Kzo9I GATC 8 cut(s) 26, 49, 265, 337, 466, 908, 1139, 1197
LmnI GCTCC 2 cut(s) 325, 981
Lsp1109I GCAGC 3 cut(s) 192, 301, 369
LweI GCATC 2 cut(s) 103, 725
MaeI CTAG 6 cut(s) 153, 374, 444, 576, 594, 1020
MaeII ACGT 3 cut(s) 167, 497, 1253
MalI GATC 8 cut(s) 28, 51, 267, 339, 468, 910, 1141, 1199
MboI GATC 8 cut(s) 26, 49, 265, 337, 466, 908, 1139, 1197
MflI RGATCY 1 cut(s) 1139
MhlI GDGCHC 1 cut(s) 695
MluCI AATT 4 cut(s) 914, 1183, 1264, 1278
MmeI TCCRAC 7 cut(s) 40, 42, 575, 1089, 1117, 1281, 1285
MseI TTAA 5 cut(s) 89, 158, 170, 758, 1238
MslI CAYNNNNRTG 1 cut(s) 706
MspA1I CMGCKG 2 cut(s) 205, 836
MspI CCGG 3 cut(s) 261, 855, 875
MspR9I CCNGG 3 cut(s) 875, 876, 1217
Mva1269I GAATGC 1 cut(s) 12
MvaI CCWGG 1 cut(s) 1217
MvnI CGCG 1 cut(s) 836
MwoI GCNNNNNNNGC 2 cut(s) 572, 1347
NciI CCSGG 2 cut(s) 875, 876
NdeII GATC 8 cut(s) 26, 49, 265, 337, 466, 908, 1139, 1197
NlaIII CATG 5 cut(s) 672, 711, 847, 955, 1109
NlaIV GGNNCC 5 cut(s) 327, 456, 1141, 1321, 1334
NspI RCATGY 2 cut(s) 711, 955
PaeI GCATGC 1 cut(s) 711
PaqCI CACCTGC 1 cut(s) 715
PcsI WCGNNNNNNNCGW 2 cut(s) 521, 1259
PctI GAATGC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 1061, 1232, 1356
PflMI CCANNNNNTGG 1 cut(s) 1083
PkrI GCNGC 5 cut(s) 207, 291, 359, 835, 838
Psp6I CCWGG 1 cut(s) 1215
PspGI CCWGG 1 cut(s) 1215
PspN4I GGNNCC 5 cut(s) 327, 456, 1141, 1321, 1334
PstI CTGCAG 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 1139
PvuII CAGCTG 1 cut(s) 205
RsaI GTAC 4 cut(s) 378, 683, 865, 886
RsaNI GTAC 4 cut(s) 377, 682, 864, 885
RseI CAYNNNNRTG 1 cut(s) 706
SacII CCGCGG 1 cut(s) 837
SaqAI TTAA 5 cut(s) 89, 158, 170, 758, 1238
SatI GCNGC 5 cut(s) 206, 290, 358, 834, 837
Sau3AI GATC 8 cut(s) 26, 49, 265, 337, 466, 908, 1139, 1197
ScrFI CCNGG 3 cut(s) 875, 876, 1217
SduI GDGCHC 1 cut(s) 695
SfaNI GCATC 2 cut(s) 103, 725
SfcI CTRYAG 1 cut(s) 33
Sfr303I CCGCGG 1 cut(s) 837
SgrBI CCGCGG 1 cut(s) 837
SmaI CCCGGG 1 cut(s) 876
SmiMI CAYNNNNRTG 1 cut(s) 706
SmlI CTYRAG 1 cut(s) 1131
SmoI CTYRAG 1 cut(s) 1131
SpeI ACTAGT 2 cut(s) 373, 443
SphI GCATGC 1 cut(s) 711
Sse9I AATT 4 cut(s) 914, 1183, 1264, 1278
SsiI CCGC 5 cut(s) 82, 208, 791, 834, 836
SspMI CTAG 6 cut(s) 153, 374, 444, 576, 594, 1020
StyD4I CCNGG 3 cut(s) 873, 874, 1215
StyI CCWWGG 3 cut(s) 320, 1242, 1336
TaaI ACNGT 2 cut(s) 542, 697
TaiI ACGT 3 cut(s) 170, 500, 1256
TaqI TCGA 6 cut(s) 216, 268, 276, 927, 993, 1122
TasI AATT 4 cut(s) 914, 1183, 1264, 1278
TatI WGTACW 2 cut(s) 376, 863
TauI GCSGC 2 cut(s) 836, 839
TfiI GAWTC 3 cut(s) 1061, 1232, 1356
Tru1I TTAA 5 cut(s) 89, 158, 170, 758, 1238
Tru9I TTAA 5 cut(s) 89, 158, 170, 758, 1238
TscAI CASTG 1 cut(s) 1006
TseI GCWGC 3 cut(s) 205, 289, 357
TspDTI ATGAA 4 cut(s) 549, 629, 786, 1284
TspMI CCCGGG 1 cut(s) 874
TspRI CASTG 1 cut(s) 1006
Van91I CCANNNNNTGG 1 cut(s) 1083
VneI GTGCAC 1 cut(s) 691
XapI RAATTY 2 cut(s) 914, 1264
XceI RCATGY 2 cut(s) 711, 955
XcmI CCANNNNNNNNNTGG 2 cut(s) 124, 1343
XmaI CCCGGG 1 cut(s) 874
XspI CTAG 6 cut(s) 153, 374, 444, 576, 594, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.