Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0010099

Physical Location & Seq

Genomic Coordinates Forward (+)

37274912 .. 37280025

Gene Structure 5114 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g49910.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3483 bp

ATGGCCTTCTCGTCCTTCAGCGCTCGCCGCGGAGGCGCGTGGCCCCACTCGCTCCTCCCCTCCACCACCGCCTCCTCCAAATCGAAGCACACCCGAAAGCCCCGGCGACGCCTCCTCCTCCGCGACTTCCTCTTCGCCAACTTCTTCACCATCGGCCTCTCCGTCTCCCTCTTCTTCTTCTTCCTCCTCCTCCTCCGCTACGGCGTCCCGCACCCGATCACCGCCGGCTTCAAGTACTCCAGGTCCCCGATCCGCTTCTCCAAGCCGCCACGTAAGCCCGTCGCCCGAAAACCCGGCCAAAACGACGACGTCTCCGGCGCCGCCGTGGACATCACCACCAAGGAGCTTTACGACAAGATTGAATTCTCCGACGTCGACGGCGGGCCGTGGAAGCAAGGATGGAGAGTCGGTTACAGAGGCGACGAGTGGGACTCTGAGAAGCTGAAGGTGGTTGTGGTGCCGCACTCGCATAACGATCCCGGTTGGAAGCTCACGGTGGCTGAGTACTATGAGAGGCAGTCCAGGCATATACTCGACACCATTGTTGCTACGCTTTCCAAGGATACTCGGCGCAAGTTTATATGGGAAGAGATGTCGTATCTGGAAAGGTGGTGGAAAGATTCCGCTGATGACAAAAGAGAATTGTTCACGAATCTGGTAAAGAATGGTCAGCTAGAGATCGTTGGAGGTGGCTGGGTGATGAATGATGAGGCTAATTCACATTACTATGCCATAATTGAACAGATCACAGAAGGAAATTTGTGGCTGAATGAAACAGTAGGTGTTATTCCTAAGAATTCTTGGGCAATAGATCCCTTTGGCTACTCATCTACCATGGCATATCTTCTGCGCCGTATGGGTTTTGAAAATATGCTCATTCAGAGAACCCATTATGAGCTTAAGAAAGAGCTTGCACTGCACAAGAATTTGGAGTATATTTGGCGTCAGAGCTGGGATGTGGATGAATCTACTGATATTTTTGTCCACATGATGCCCTTTTATTCTTATGATGTTCCACATACTTGTGGGCCAGAGCCAGCTATTTGTTGTCAGTTCGACTTTGCTCGTATGCGTGGCTTTATGTATGAATTTTGTCCTTGGGGAGATAATCCGGTAGAGACCAACCAAGAAAATGTGCAGGAGCGGGCACTTCTACTACTAGATCAATATAAGAAGAAATCAACACTATACAGGACAAATACACTTCTTATTCCTCTTGGAGATGATTTTCGCTACGTTAGCATAGAGGAAGCTGAAGCTCAGTTTAGGAATTATCAGATGTTATTTGATTATATCAATTCTAATCCCAGTTTAAATGCTGAGGCGCATTTTGGAACTTTGGAGGACTACTTTCGAACCCTTCGTGAGGAGGCTGAAAGAATAAATCACACACGTCCTGGGGAAATTGGTTCCGGTCAGGTTGGAGGTTTTCCTTCTCTTTCGGGTGACTTCTTCACTTATGCTGATAGGCAGCAGGACTATTGGAGTGGTTATTATGTATCAAGGCCTTTCTTCAAGGCTGTTGATCGAGTACTAGAGCATACACTTCGTGCCACGGATATGATGATGGCTTTCCTGCTTGGGTACTGTGGGAGAGCACAATGTGAAAAGTTGCCCATCGGGTTTTCTTACAAGCTAGCAGCTGCTAGAAGGAATTTAGCTCTTTTTCAGCATCATGATGGTGTGACTGGTACCGCTAAAGATCATGTTGTCCTGGATTATGGGATGCGGATGCACACTTCCTTGCAGGACTTGCAGATTTTCATGTCTAAAGCTATTGAGGTGTTGCTTGGAATACGCCATGACAAATATGATATTAACCCTTCTCAGTTTGAGCCAGAACAGGTGAGATCTAAATATGATGTTCAGCCTGTTCATAGAGCGATCATGGCTCGTGAAGGAACCCGCCAAACAGTAGTCCTTTTCAATCCCTCGGAGCAGATAAGAGAAGAGGTTGTGATGGTTATTGTTAACCGTCCTGATGTTACTGTTCTAGACTTGAACTGGACATGTGTCCCAAGCCAAATATCCCCTGAATTGCAGCATGATAAAAGCAAAATCTTCACTGGAAGGCATCGTGTCTACTGGCAGGCTTCTGTTCCTGCTCTGGGGTTGCAGACATATTATATAACTAATGGATTTGCTGGATGTGAAAAGGCGAAGCCAGCAAAAATTAGATACTTCTCAAAGTCTGGTTCTTTCTCTTGCCCAACTCCATATCCTTGTTCGAAAGTAGAAGCTGATGTGGCTGAAATCCAGAACAGGCATCAGACACTCACTTTTGATGTCAATCATGGTCTATTGCAGAAAATAAGCTATAAAACTGGGACCCAAAATGTTGTGGGTGAAGAAATAGCTATGTATTCTAGCTGGGGAAGCGGAGCATATCTTTTTAAACCTGATGGCGATGCGCAGCCTATCATTGCAGCAGGTGGGCAGATGGTGATCTCTGAGGGACCTTTGGTGCAGGAAGTATATTCTTATCCAAGTACACAATGGGAGAAGAGCCCCATTTCTCATAGCACTCGTCTCTACAACGGAGAAAATACTGTACAGGAGTTTCTCATTGAGAAGGAGTATCATGTTGAGCTTCTCGACCAGCAGTTTAATGACAGGGAGTTGATAGTTAGATACAAAACAGATATTGACAACAAAAGAGTATTCTTTTCTGATTTAAATGGCTTTCAGATGAGCAGGAGAGAAACCTACAATAAAATCCCCCTTCAGGGCAATTACTACCCTATGCCTTCTCTTGCATTCATGCAGGGATCCAATGGTCAGCGGTTTTCTGTTCATTCCCGGCAGTCATTGGGTGTGGCAAGCCTTAAAAATGGATGGTTGGAGATTATGCTTGACCGTCGTTTGGTAAGAGATGACGGACGTGGTCTTGGACAAGGAGTGATGGACAACCGTGCGATGAATGTAGTCTTCCACATCCTTGTAGAGGCCAATATTTCTTCTGCATCAAACCCAGTTTCTAACCCATTGCCATTAAACCCCTCTCTTCTCTCCCACCGTGTCGGTGCTGACCTGAACTATCCATTGCATGCATTCGTTTCCAAGAAGCCAGAGGATTTGTCAGTGCAACCACCCCTGAGATCATTCTCACCCTTAGCTGCACCCTTACCGTGTGATCTGCATATTGTAAGTTTAAAGGTTCCCCAACCTTTAAAGTTCTCTCAGCCACCACTTGAAGATTCTAGATTTGTCTTAACATTACAGAGACGAAGTTGGGACTCTTCATATTGCCGGAAGGGCAGGTCTAATTGCACTAGATTTGCTGATGAAACTGTGAATTTGCTCAACATGTTTCGGGAGCTCACTGTATCGAATGGGAGACCAACTTCCTTAAATCTTCTACATGAAGACACAGATATGCTTGGGTATCCTGAGCAGTTTGGAGATGTTGCTGCAGAAGGACAAGTCCTCATTTCCCCCATGGAAATACAGGCTTACAAGATGGAGTTACAGCCACACCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0004572 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006486 GO:0006487 GO:0006491 GO:0006517 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0015923 GO:0015924 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0034645 GO:0036211 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0070085 GO:0071704 GO:0097708 GO:0098588 GO:0098791 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko00513 ko01100 map00510 map00513 map01100

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1161

Amino Acids

133.01

Weight (kDa)

6.73

Isoelectric Point (pI)

44.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	149 - 250	2.2e-32	Glycosyl hydrolases family 38 N-terminal domain
Glyco_hydro_38N	PF01074	287 - 526	3.1e-43	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	533 - 635	1.5e-20	Alpha mannosidase middle domain
Glyco_hydro_38C	PF07748	791 - 1000	1e-31	Glycosyl hydrolases family 38 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010099)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14950
fragaria_vesca	FvH4_6g49910 FvH4_6g49910
malus_domestica	MD09G1040900.v1.1 MD17G1042200.v1.1
prunus_persica	Prupe.3G276800_v2.0.a1
pyrus_communis	pycom111g03190 pycom17g03770
rosa_chinensis	RchiOBHm_Chr2g0170081
rosa_laevigata	RLG00000021920
rosa_multiflora	Rmu_co8321257.1_g000001 Rmu_sc0013078.1_g000007 Rmu_sc0013453.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081600
rosa_rugosa	Rorug02G0545500 Rorug02G0545600.1
rosa_samantha	Rh2AG617700 Rh2BG629200 Rh2CG598600 Rh2DG641000
rosa_wichuraiana	Rw2G051140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2420
AatII	GACGTC	2 cut(s)	312, 375
Acc16I	TGCGCA	1 cut(s)	2412
Acc36I	ACCTGC	2 cut(s)	2420, 3249
Acc65I	GGTACC	1 cut(s)	1691
AccB1I	GGYRCC	3 cut(s)	317, 457, 1691
AccBSI	CCGCTC	1 cut(s)	1144
AccI	GTMKAC	2 cut(s)	375, 2082
AccII	CGCG	3 cut(s)	30, 38, 123
AclWI	GGATC	5 cut(s)	244, 470, 806, 2763, 2776
AcoI	YGGCCR	1 cut(s)	295
AcsI	RAATTY	7 cut(s)	362, 757, 796, 925, 1088, 1654, 3295
AcuI	CTGAAG	3 cut(s)	464, 1275, 2708
AcyI	GRCGYC	6 cut(s)	109, 204, 309, 318, 372, 943
AdeI	CACNNNGTG	2 cut(s)	1550, 1604
AfaI	GTAC	7 cut(s)	236, 506, 1533, 1586, 1693, 2491, 2553
AfeI	AGCGCT	1 cut(s)	22
AfiI	CCNNNNNNNGG	6 cut(s)	1366, 2108, 2725, 2726, 2863, 2944
AflII	CTTAAG	1 cut(s)	899
AflIII	ACRYGT	3 cut(s)	1391, 2009, 3306
AgsI	TTSAA	8 cut(s)	232, 362, 740, 866, 1516, 1927, 2002, 3194
AjiI	CACGTC	2 cut(s)	1394, 2882
AjnI	CCWGG	4 cut(s)	239, 521, 1396, 1713
AleI	CACNNNNGTG	1 cut(s)	3478
Alw21I	GWGCWC	2 cut(s)	1600, 3321
Alw26I	GTCTC	6 cut(s)	169, 316, 1112, 2534, 3217, 3331
AlwI	GGATC	5 cut(s)	244, 470, 806, 2763, 2776
Aor51HI	AGCGCT	1 cut(s)	22
AoxI	GGCC	8 cut(s)	3, 41, 154, 295, 383, 1028, 1505, 2946
ApeKI	GCWGC	8 cut(s)	1471, 1640, 1643, 2041, 2413, 2426, 3116, 3410
ApoI	RAATTY	7 cut(s)	362, 757, 796, 925, 1088, 1654, 3295
ArsI	GACNNNNNNTTYG	2 cut(s)	116, 148
Asp718I	GGTACC	1 cut(s)	1691
AspLEI	GCGC	7 cut(s)	23, 38, 320, 573, 852, 1327, 2413
AspS9I	GGNCC	6 cut(s)	42, 243, 383, 1028, 2328, 2456
AsuC2I	CCSGG	4 cut(s)	103, 294, 480, 2800
AsuHPI	GGTGA	9 cut(s)	139, 211, 325, 709, 1457, 1858, 2357, 2455, 3099
AsuII	TTCGAA	2 cut(s)	1354, 2228
AsuNHI	GCTAGC	1 cut(s)	1636
AvaII	GGWCC	3 cut(s)	243, 2328, 2456
BaeGI	GKGCMC	1 cut(s)	1150
BamHI	GGATCC	1 cut(s)	2768
BanI	GGYRCC	3 cut(s)	317, 457, 1691
BanII	GRGCYC	2 cut(s)	2510, 3321
BauI	CACGAG	1 cut(s)	1893
BbsI	GAAGAC	2 cut(s)	2920, 3372
Bbv12I	GWGCWC	2 cut(s)	1600, 3321
BbvCI	CCTCAGC	1 cut(s)	1320
BbvI	GCAGC	8 cut(s)	1483, 1630, 1652, 2053, 2425, 2438, 3103, 3397
BceAI	ACGGC	5 cut(s)	217, 308, 370, 394, 837
BcgI	CGANNNNNNTGC	2 cut(s)	1529, 1563
BciT130I	CCWGG	4 cut(s)	241, 523, 1398, 1715
BciVI	GTATCC	2 cut(s)	556, 3396
BcnI	CCSGG	4 cut(s)	103, 294, 480, 2800
BcoDI	GTCTC	6 cut(s)	169, 316, 1112, 2534, 3217, 3331
BfaI	CTAG	9 cut(s)	674, 1160, 1535, 1637, 1647, 1994, 2367, 3201, 3273
BfmI	CTRYAG	1 cut(s)	3411
BfoI	RGCGCY	2 cut(s)	24, 321
BfrI	CTTAAG	1 cut(s)	899
BfuAI	ACCTGC	2 cut(s)	2420, 3249
BfuI	GTATCC	2 cut(s)	556, 3396
BglI	GCCNNNNNGGC	2 cut(s)	33, 3255
BglII	AGATCT	1 cut(s)	1850
BmcAI	AGTACT	3 cut(s)	236, 506, 1533
Bme1390I	CCNGG	8 cut(s)	103, 241, 294, 480, 523, 1398, 1715, 2800
Bme18I	GGWCC	3 cut(s)	243, 2328, 2456
BmgBI	CACGTC	2 cut(s)	1394, 2882
BmgT120I	GGNCC	6 cut(s)	42, 243, 383, 1028, 2328, 2456
BmrFI	CCNGG	8 cut(s)	103, 241, 294, 480, 523, 1398, 1715, 2800
BmrI	ACTGGG	3 cut(s)	1302, 2334, 2966
BmsI	GCATC	8 cut(s)	981, 1681, 1716, 1722, 2083, 2275, 2398, 2972
BmtI	GCTAGC	1 cut(s)	1640
BmuI	ACTGGG	3 cut(s)	1302, 2334, 2966
BoxI	GACNNNNGTC	1 cut(s)	2012
BpiI	GAAGAC	2 cut(s)	2920, 3372
BplI	GAGNNNNNCTC	2 cut(s)	416, 448
BpmI	CTGGAG	1 cut(s)	223
Bpu10I	CCTNAGC	3 cut(s)	1320, 3112, 3390
Bpu14I	TTCGAA	2 cut(s)	1354, 2228
BpuMI	CCSGG	4 cut(s)	103, 294, 480, 2800
BsaAI	YACGTR	1 cut(s)	272
BsaBI	GATNNNNATC	2 cut(s)	2289, 2444
BsaHI	GRCGYC	6 cut(s)	109, 204, 309, 318, 372, 943
BsaI	GGTCTC	2 cut(s)	1112, 3331
BsaWI	WCCGGW	2 cut(s)	1111, 1412
BsaXI	ACNNNNNCTCC	6 cut(s)	99, 129, 296, 326, 394, 424
Bsc4I	CCNNNNNNNGG	6 cut(s)	1366, 2108, 2725, 2726, 2863, 2944
Bse118I	RCCGGY	1 cut(s)	224
Bse1I	ACTGG	8 cut(s)	1308, 1693, 2009, 2071, 2090, 2329, 2972, 3478
Bse3DI	GCAATG	3 cut(s)	2421, 2984, 3041
Bse8I	GATNNNNATC	2 cut(s)	2289, 2444
BseBI	CCWGG	4 cut(s)	241, 523, 1398, 1715
BseGI	GGATG	8 cut(s)	404, 961, 967, 1731, 1737, 2153, 2840, 2934
BseJI	GATNNNNATC	2 cut(s)	2289, 2444
BseLI	CCNNNNNNNGG	6 cut(s)	1366, 2108, 2725, 2726, 2863, 2944
BseMI	GCAATG	3 cut(s)	2421, 2984, 3041
BseMII	CTCAG	9 cut(s)	426, 492, 1274, 1311, 1841, 2442, 3086, 3194, 3381
BseNI	ACTGG	8 cut(s)	1308, 1693, 2009, 2071, 2090, 2329, 2972, 3478
BseRI	GAGGAG	8 cut(s)	44, 64, 104, 107, 176, 179, 182, 1382
BseSI	GKGCMC	1 cut(s)	1150
BseXI	GCAGC	8 cut(s)	1483, 1630, 1652, 2053, 2425, 2438, 3103, 3397
BseYI	CCCAGC	3 cut(s)	693, 951, 2370
BsgI	GTGCAG	4 cut(s)	902, 1157, 2486, 3102
Bsh1236I	CGCG	3 cut(s)	30, 38, 123
BshFI	GGCC	8 cut(s)	5, 43, 156, 297, 385, 1030, 1507, 2948
BshNI	GGYRCC	3 cut(s)	317, 457, 1691
BsiHKAI	GWGCWC	2 cut(s)	1600, 3321
BsiSI	CCGG	9 cut(s)	103, 225, 294, 315, 480, 1112, 1413, 2800, 3250
BslFI	GGGAC	7 cut(s)	191, 229, 443, 2000, 2341, 2469, 3248
BslI	CCNNNNNNNGG	6 cut(s)	1366, 2108, 2725, 2726, 2863, 2944
BsmAI	GTCTC	6 cut(s)	169, 316, 1112, 2534, 3217, 3331
BsmBI	CGTCTC	4 cut(s)	169, 316, 2534, 3217
BsmFI	GGGAC	7 cut(s)	191, 229, 443, 2000, 2341, 2469, 3248
BsmI	GAATGC	2 cut(s)	2756, 3050
BsnI	GGCC	8 cut(s)	5, 43, 156, 297, 385, 1030, 1507, 2948
Bso31I	GGTCTC	2 cut(s)	1112, 3331
Bsp119I	TTCGAA	2 cut(s)	1354, 2228
Bsp1286I	GDGCHC	4 cut(s)	1150, 1600, 2510, 3321
Bsp1407I	TGTACA	1 cut(s)	2551
Bsp19I	CCATGG	2 cut(s)	834, 3438
BspANI	GGCC	8 cut(s)	5, 43, 156, 297, 385, 1030, 1507, 2948
BspCNI	CTCAG	9 cut(s)	427, 493, 1273, 1312, 1840, 2443, 3087, 3193, 3382
BspFNI	CGCG	3 cut(s)	30, 38, 123
BspHI	TCATGA	1 cut(s)	1675
BspMAI	CTGCAG	1 cut(s)	3415
BspMI	ACCTGC	2 cut(s)	2420, 3249
BspOI	GCTAGC	1 cut(s)	1640
BspPI	GGATC	5 cut(s)	244, 470, 806, 2763, 2776
BspQI	GCTCTTC	1 cut(s)	2498
BspT104I	TTCGAA	2 cut(s)	1354, 2228
BspT107I	GGYRCC	3 cut(s)	317, 457, 1691
BspTI	CTTAAG	1 cut(s)	899
BspTNI	GGTCTC	2 cut(s)	1112, 3331
BsrBI	CCGCTC	1 cut(s)	1144
BsrDI	GCAATG	3 cut(s)	2421, 2984, 3041
BsrFI	RCCGGY	1 cut(s)	224
BsrGI	TGTACA	1 cut(s)	2551
BsrI	ACTGG	8 cut(s)	1308, 1693, 2009, 2071, 2090, 2329, 2972, 3478
BssAI	RCCGGY	1 cut(s)	224
BssNI	GRCGYC	6 cut(s)	109, 204, 309, 318, 372, 943
BssSI	CACGAG	1 cut(s)	1893
BssT1I	CCWWGG	5 cut(s)	339, 558, 834, 1097, 3438
Bst2BI	CACGAG	1 cut(s)	1893
Bst2UI	CCWGG	4 cut(s)	241, 523, 1398, 1715
Bst6I	CTCTTC	7 cut(s)	137, 176, 582, 1944, 2498, 3009, 3244
BstACI	GRCGYC	6 cut(s)	109, 204, 309, 318, 372, 943
BstAFI	CTTAAG	1 cut(s)	899
BstAPI	GCANNNNNTGC	1 cut(s)	1753
BstAUI	TGTACA	1 cut(s)	2551
BstBAI	YACGTR	1 cut(s)	272
BstBI	TTCGAA	2 cut(s)	1354, 2228
BstDSI	CCRYGG	6 cut(s)	28, 324, 386, 834, 1554, 3438
BstENI	CCTNNNNNAGG	2 cut(s)	1364, 2942
BstF5I	GGATG	8 cut(s)	404, 961, 967, 1731, 1737, 2153, 2840, 2934
BstFNI	CGCG	3 cut(s)	30, 38, 123
BstH2I	RGCGCY	2 cut(s)	24, 321
BstHHI	GCGC	7 cut(s)	23, 38, 320, 573, 852, 1327, 2413
BstMAI	GTCTC	6 cut(s)	169, 316, 1112, 2534, 3217, 3331
BstNI	CCWGG	4 cut(s)	241, 523, 1398, 1715
BstNSI	RCATGY	3 cut(s)	2013, 3050, 3310
BstPAI	GACNNNNGTC	1 cut(s)	2012
BstSCI	CCNGG	8 cut(s)	101, 239, 292, 478, 521, 1396, 1713, 2798
BstSFI	CTRYAG	1 cut(s)	3411
BstSLI	GKGCMC	1 cut(s)	1150
BstUI	CGCG	3 cut(s)	30, 38, 123
BstV1I	GCAGC	8 cut(s)	1483, 1630, 1652, 2053, 2425, 2438, 3103, 3397
BstV2I	GAAGAC	2 cut(s)	2920, 3372
BstX2I	RGATCY	3 cut(s)	811, 1850, 2768
BstYI	RGATCY	3 cut(s)	811, 1850, 2768
BsuI	GTATCC	2 cut(s)	556, 3396
BsuRI	GGCC	8 cut(s)	5, 43, 156, 297, 385, 1030, 1507, 2948
BtgI	CCRYGG	6 cut(s)	28, 324, 386, 834, 1554, 3438
BtgZI	GCGATG	2 cut(s)	2421, 2930
BtrI	CACGTC	2 cut(s)	1394, 2882
BtsCI	GGATG	8 cut(s)	404, 961, 967, 1731, 1737, 2153, 2840, 2934
BtsI	GCAGTG	1 cut(s)	914
BtsIMutI	CAGTG	4 cut(s)	914, 2064, 3087, 3321
BveI	ACCTGC	2 cut(s)	2420, 3249
CciI	TCATGA	1 cut(s)	1675
CfoI	GCGC	7 cut(s)	23, 38, 320, 573, 852, 1327, 2413
Cfr10I	RCCGGY	1 cut(s)	224
Cfr13I	GGNCC	6 cut(s)	42, 243, 383, 1028, 2328, 2456
Cfr42I	CCGCGG	1 cut(s)	31
CseI	GACGC	3 cut(s)	117, 193, 932
Csp6I	GTAC	7 cut(s)	235, 505, 1532, 1585, 1692, 2490, 2552
CviQI	GTAC	7 cut(s)	235, 505, 1532, 1585, 1692, 2490, 2552
DinI	GGCGCC	1 cut(s)	319
DraI	TTTAAA	5 cut(s)	1314, 2395, 2676, 3153, 3171
DraIII	CACNNNGTG	2 cut(s)	1550, 1604
EaeI	YGGCCR	1 cut(s)	295
Eam1104I	CTCTTC	7 cut(s)	137, 176, 582, 1944, 2498, 3009, 3244
EarI	CTCTTC	7 cut(s)	137, 176, 582, 1944, 2498, 3009, 3244
Ecl136II	GAGCTC	1 cut(s)	3319
Eco130I	CCWWGG	5 cut(s)	339, 558, 834, 1097, 3438
Eco147I	AGGCCT	1 cut(s)	1507
Eco24I	GRGCYC	2 cut(s)	2510, 3321
Eco31I	GGTCTC	2 cut(s)	1112, 3331
Eco47I	GGWCC	3 cut(s)	243, 2328, 2456
Eco47III	AGCGCT	1 cut(s)	22
Eco53kI	GAGCTC	1 cut(s)	3319
Eco57I	CTGAAG	3 cut(s)	464, 1275, 2708
EcoICRI	GAGCTC	1 cut(s)	3319
EcoNI	CCTNNNNNAGG	2 cut(s)	1364, 2942
EcoO109I	RGGNCCY	3 cut(s)	243, 2328, 2456
EcoRI	GAATTC	2 cut(s)	362, 796
EcoRII	CCWGG	4 cut(s)	239, 521, 1396, 1713
EcoT14I	CCWWGG	5 cut(s)	339, 558, 834, 1097, 3438
EcoT22I	ATGCAT	1 cut(s)	3052
EcoT38I	GRGCYC	2 cut(s)	2510, 3321
EgeI	GGCGCC	1 cut(s)	319
EheI	GGCGCC	1 cut(s)	319
ErhI	CCWWGG	5 cut(s)	339, 558, 834, 1097, 3438
Esp3I	CGTCTC	4 cut(s)	169, 316, 2534, 3217
FaqI	GGGAC	7 cut(s)	191, 229, 443, 2000, 2341, 2469, 3248
FauI	CCCGC	4 cut(s)	216, 374, 1137, 1913
FblI	GTMKAC	2 cut(s)	375, 2082
FokI	GGATG	8 cut(s)	411, 968, 974, 1738, 1744, 2160, 2847, 2921
FriOI	GRGCYC	2 cut(s)	2510, 3321
FspBI	CTAG	9 cut(s)	674, 1160, 1535, 1637, 1647, 1994, 2367, 3201, 3273
FspI	TGCGCA	1 cut(s)	2412
GlaI	GCGC	7 cut(s)	22, 37, 319, 572, 851, 1326, 2412
GsaI	CCCAGC	3 cut(s)	697, 955, 2374
GsuI	CTGGAG	1 cut(s)	223
HaeII	RGCGCY	2 cut(s)	24, 321
HaeIII	GGCC	8 cut(s)	5, 43, 156, 297, 385, 1030, 1507, 2948
HapII	CCGG	9 cut(s)	103, 225, 294, 315, 480, 1112, 1413, 2800, 3250
HgaI	GACGC	3 cut(s)	117, 193, 932
HhaI	GCGC	7 cut(s)	23, 38, 320, 573, 852, 1327, 2413
Hin1I	GRCGYC	6 cut(s)	109, 204, 309, 318, 372, 943
Hin6I	GCGC	7 cut(s)	21, 36, 318, 571, 850, 1325, 2411
HinP1I	GCGC	7 cut(s)	21, 36, 318, 571, 850, 1325, 2411
HincII	GTYRAC	2 cut(s)	376, 1972
HindII	GTYRAC	2 cut(s)	376, 1972
HinfI	GANTC	7 cut(s)	405, 431, 620, 652, 965, 3197, 3236
HpaI	GTTAAC	1 cut(s)	1972
HpaII	CCGG	9 cut(s)	103, 225, 294, 315, 480, 1112, 1413, 2800, 3250
HphI	GGTGA	9 cut(s)	139, 211, 325, 709, 1457, 1858, 2357, 2455, 3099
Hpy166II	GTNNAC	7 cut(s)	328, 376, 648, 985, 1972, 2083, 2492
Hpy8I	GTNNAC	7 cut(s)	328, 376, 648, 985, 1972, 2083, 2492
Hpy99I	CGWCG	9 cut(s)	111, 284, 308, 311, 374, 377, 380, 425, 2862
HpyCH4IV	ACGT	6 cut(s)	271, 309, 372, 1236, 1393, 2881
HpySE526I	ACGT	6 cut(s)	271, 309, 372, 1236, 1393, 2881
Hsp92I	GRCGYC	6 cut(s)	109, 204, 309, 318, 372, 943
HspAI	GCGC	7 cut(s)	21, 36, 318, 571, 850, 1325, 2411
KasI	GGCGCC	1 cut(s)	317
KflI	GGGWCCC	1 cut(s)	2328
KpnI	GGTACC	1 cut(s)	1695
KroI	GCCGGC	1 cut(s)	224
KroNI	GCCGGC	1 cut(s)	226
KspAI	GTTAAC	1 cut(s)	1972
KspI	CCGCGG	1 cut(s)	31
LguI	GCTCTTC	1 cut(s)	2498
LmnI	GCTCC	6 cut(s)	57, 343, 1141, 1936, 2381, 3316
Lsp1109I	GCAGC	8 cut(s)	1483, 1630, 1652, 2053, 2425, 2438, 3103, 3397
LweI	GCATC	8 cut(s)	981, 1681, 1716, 1722, 2083, 2275, 2398, 2972
MaeI	CTAG	9 cut(s)	674, 1160, 1535, 1637, 1647, 1994, 2367, 3201, 3273
MaeII	ACGT	6 cut(s)	271, 309, 372, 1236, 1393, 2881
MaeIII	GTNAC	5 cut(s)	410, 1445, 1684, 1984, 3465
MbiI	CCGCTC	1 cut(s)	1144
MflI	RGATCY	3 cut(s)	811, 1850, 2768
MhlI	GDGCHC	4 cut(s)	1150, 1600, 2510, 3321
Mly113I	GGCGCC	1 cut(s)	318
MlyI	GAGTC	3 cut(s)	414, 425, 3230
MmeI	TCCRAC	5 cut(s)	393, 464, 664, 1402, 2820
Mph1103I	ATGCAT	1 cut(s)	3052
MroNI	GCCGGC	1 cut(s)	224
MslI	CAYNNNNRTG	7 cut(s)	726, 1023, 1559, 1677, 1680, 3374, 3478
MspA1I	CMGCKG	4 cut(s)	30, 626, 1643, 2782
MspCI	CTTAAG	1 cut(s)	899
MspI	CCGG	9 cut(s)	103, 225, 294, 315, 480, 1112, 1413, 2800, 3250
MspR9I	CCNGG	8 cut(s)	103, 241, 294, 480, 523, 1398, 1715, 2800
Mva1269I	GAATGC	2 cut(s)	2756, 3050
MvaI	CCWGG	4 cut(s)	241, 523, 1398, 1715
MvnI	CGCG	3 cut(s)	30, 38, 123
NaeI	GCCGGC	1 cut(s)	226
NarI	GGCGCC	1 cut(s)	318
NciI	CCSGG	4 cut(s)	103, 294, 480, 2800
NcoI	CCATGG	2 cut(s)	834, 3438
NgoMIV	GCCGGC	1 cut(s)	224
NheI	GCTAGC	1 cut(s)	1636
NmeAIII	GCCGAG	1 cut(s)	547
NmuCI	GTSAC	2 cut(s)	1445, 1684
NsbI	TGCGCA	1 cut(s)	2412
NsiI	ATGCAT	1 cut(s)	3052
NspI	RCATGY	3 cut(s)	2013, 3050, 3310
NspV	TTCGAA	2 cut(s)	1354, 2228
OliI	CACNNNNGTG	1 cut(s)	3478
PaeI	GCATGC	1 cut(s)	3050
PagI	TCATGA	1 cut(s)	1675
PaqCI	CACCTGC	1 cut(s)	2420
PceI	AGGCCT	1 cut(s)	1507
PciI	ACATGT	2 cut(s)	2009, 3306
PciSI	GCTCTTC	1 cut(s)	2498
PcsI	WCGNNNNNNNCGW	2 cut(s)	159, 1360
PctI	GAATGC	2 cut(s)	2756, 3050
PdiI	GCCGGC	1 cut(s)	226
PfeI	GAWTC	4 cut(s)	620, 652, 965, 3197
PflFI	GACNNNGTC	2 cut(s)	308, 2883
PfoI	TCCNGGA	1 cut(s)	1713
PleI	GAGTC	3 cut(s)	413, 425, 3230
PluTI	GGCGCC	1 cut(s)	321
PpsI	GAGTC	3 cut(s)	413, 425, 3230
Ppu21I	YACGTR	1 cut(s)	272
PpuMI	RGGWCCY	3 cut(s)	243, 2328, 2456
PscI	ACATGT	2 cut(s)	2009, 3306
PshAI	GACNNNNGTC	1 cut(s)	2012
Psp124BI	GAGCTC	1 cut(s)	3321
Psp5II	RGGWCCY	3 cut(s)	243, 2328, 2456
Psp6I	CCWGG	4 cut(s)	239, 521, 1396, 1713
PspFI	CCCAGC	3 cut(s)	693, 951, 2370
PspGI	CCWGG	4 cut(s)	239, 521, 1396, 1713
PspPI	GGNCC	6 cut(s)	42, 243, 383, 1028, 2328, 2456
PspPPI	RGGWCCY	3 cut(s)	243, 2328, 2456
PstI	CTGCAG	1 cut(s)	3415
PsuI	RGATCY	3 cut(s)	811, 1850, 2768
PsyI	GACNNNGTC	2 cut(s)	308, 2883
PvuII	CAGCTG	1 cut(s)	1643
RsaI	GTAC	7 cut(s)	236, 506, 1533, 1586, 1693, 2491, 2553
RsaNI	GTAC	7 cut(s)	235, 505, 1532, 1585, 1692, 2490, 2552
RseI	CAYNNNNRTG	7 cut(s)	726, 1023, 1559, 1677, 1680, 3374, 3478
SacI	GAGCTC	1 cut(s)	3321
SacII	CCGCGG	1 cut(s)	31
SalI	GTCGAC	1 cut(s)	374
SapI	GCTCTTC	1 cut(s)	2498
Sau96I	GGNCC	6 cut(s)	42, 243, 383, 1028, 2328, 2456
ScaI	AGTACT	3 cut(s)	236, 506, 1533
SchI	GAGTC	3 cut(s)	414, 425, 3230
ScrFI	CCNGG	8 cut(s)	103, 241, 294, 480, 523, 1398, 1715, 2800
SduI	GDGCHC	4 cut(s)	1150, 1600, 2510, 3321
SfaNI	GCATC	8 cut(s)	981, 1681, 1716, 1722, 2083, 2275, 2398, 2972
SfcI	CTRYAG	1 cut(s)	3411
SfoI	GGCGCC	1 cut(s)	319
Sfr303I	CCGCGG	1 cut(s)	31
SfuI	TTCGAA	2 cut(s)	1354, 2228
SgrBI	CCGCGG	1 cut(s)	31
SgrDI	CGTCGACG	1 cut(s)	374
SinI	GGWCC	3 cut(s)	243, 2328, 2456
SmiI	ATTTAAAT	1 cut(s)	2676
SmiMI	CAYNNNNRTG	7 cut(s)	726, 1023, 1559, 1677, 1680, 3374, 3478
SmlI	CTYRAG	1 cut(s)	899
SmoI	CTYRAG	1 cut(s)	899
SphI	GCATGC	1 cut(s)	3050
SseBI	AGGCCT	1 cut(s)	1507
SspDI	GGCGCC	1 cut(s)	317
SspI	AATATT	1 cut(s)	2953
SspMI	CTAG	9 cut(s)	674, 1160, 1535, 1637, 1647, 1994, 2367, 3201, 3273
SstI	GAGCTC	1 cut(s)	3321
StuI	AGGCCT	1 cut(s)	1507
StyD4I	CCNGG	8 cut(s)	101, 239, 292, 478, 521, 1396, 1713, 2798
StyI	CCWWGG	5 cut(s)	339, 558, 834, 1097, 3438
SwaI	ATTTAAAT	1 cut(s)	2676
TaiI	ACGT	6 cut(s)	274, 312, 375, 1239, 1396, 2884
TaqI	TCGA	9 cut(s)	83, 375, 534, 1056, 1354, 1528, 2228, 2595, 3329
TatI	WGTACW	5 cut(s)	234, 504, 1531, 2489, 2551
TauI	GCSGC	4 cut(s)	30, 268, 323, 463
TfiI	GAWTC	4 cut(s)	620, 652, 965, 3197
TscAI	CASTG	4 cut(s)	921, 2071, 3087, 3328
TseFI	GTSAC	2 cut(s)	1445, 1684
TseI	GCWGC	8 cut(s)	1471, 1640, 1643, 2041, 2413, 2426, 3116, 3410
Tsp45I	GTSAC	2 cut(s)	1445, 1684
TspGWI	ACGGA	4 cut(s)	151, 1571, 2553, 2892
TspRI	CASTG	4 cut(s)	921, 2071, 3087, 3328
Tth111I	GACNNNGTC	2 cut(s)	308, 2883
Vha464I	CTTAAG	1 cut(s)	899
VpaK11BI	GGWCC	3 cut(s)	243, 2328, 2456
XagI	CCTNNNNNAGG	2 cut(s)	1364, 2942
XapI	RAATTY	7 cut(s)	362, 757, 796, 925, 1088, 1654, 3295
XbaI	TCTAGA	2 cut(s)	1993, 3200
XceI	RCATGY	3 cut(s)	2013, 3050, 3310
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2493
XmiI	GTMKAC	2 cut(s)	375, 2082
XspI	CTAG	9 cut(s)	674, 1160, 1535, 1637, 1647, 1994, 2367, 3201, 3273
ZraI	GACGTC	2 cut(s)	310, 373
ZrmI	AGTACT	3 cut(s)	236, 506, 1533
Zsp2I	ATGCAT	1 cut(s)	3052

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.