Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0010099

Physical Location & Seq

Genomic Coordinates Forward (+)

86859867 .. 86864755

Gene Structure 4889 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG641000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGGATCAGATCACAGAAGGAAATTTGTGGCTGAATGAAACCGTGGGTGTTGTTCCTAAGAATTCGTGGGCAATAGATCCATTTGGTTACTCACCCACCATGGCATATCTTCTGCGCCGTATGGGTTTCGAAAATATGCTCATTCAGAGGACCCATTATGAGCTTAAGAAAGAACTTGCACTGCACAAGAATTTGGAGTATATATGGCGTCAGAGCTGGGATGTTGATGAATCTACTGATATTTTTGTCCACATGATGCCCTTTTATTCTTATGATATTCCACATACTTGTGGGCCAGAGCCAGCTATTTGTTGTCAGTTCGACTTTGCTCGTACGCGTGGCTCTATGTATGAACTTTGTCCTTGGGGAGACCATCCAGTAGAGACTAACCAAGAAAATGTGCGGGAGCGGGCACTTCTACTACTAGATCAATATAGGAAGAAATCAACACTGTACAGGACAAATACACTTCTTATTCCTCTTGGAGATGATTTCCGCTACGTTAGCATAGATGAAGCTGAAGCTCAGTTTAGGAATTATCAAATGTTATTTGATTATATCAATTCTAATCCCAGTTTAAATGCTGAGGCGCATTTTGGAACTTTGGAAGACTACTTTCGAACCATTCGTGAGGAGGCTGAAAGAATAAATTACTCACTTCCTGGGGAGATTGGTTCCGGTCAGGTTGGAGGTTTTCCTTCTTTGTCGGGTGACTTCTTTACTTACGCTGATAGGCAACAGGACTATTGGAGTGGTTATTATGTATCAAGGCCTTTCTTCAAGGCTGTTGATCGAGTACTAGAGCATACGCTTCGTACCACGGATATGATGATGGCTTTCCTGCTTGGGTACTGTGGGAGATCACAATGCGAAAAGTTACCCTTGGGGTTTTCCTACAAGTTGGCAGCTGCTAGAAGGAATTTAGCTCTTTTTCAGCATCATGATGGTGTAACTGGTACTGCTAAAGATCATGTTGTCCTGGATTATGGGACTCGGATGCACACTTCCTTGCAGGACTTGCAGATTTTCATGTCTAAAGCTATTGAAGTGTTGCTTGGAATACGCCATGACAAATATGATAACCCTTCTCAGTTTGAGGCAGAACAGGTGAGATCTAAATATGATGTTCAGCCTGTTCATAGAGCAATCATGGCTCGTGAAGGAACCCGCCAAACAGTGGTCCTTTTTAATCCCTTGGAGCAGATAAGAGAAGAGGTTGTGATGGTTATTGTTAACCGTCCCGATGTTACTGTTCTGAACTTGAACTGGACATGTGTCCCAAGCCAAATATCGCCTGAATTGCAGCATGATAAAAGCAAAATCTTCACTGGAAGGCATCGTGTCTACTGGCAGGCTTCTGTTCCTGCTCTGGGGTTGCAGACATATTATATAACTAATGGGTTTGTTGGATGTGAAAAGGCGAAACCAGCAAAACTTAGATACTTCTCAAAGTCTAGTTCTTTCTCTTGCCCCACTCCATATCCTTGTTCGAAAGTAGAAGCTGATGTGGCTGAAATCAAGAACAGGCATCAAACACTCACTTTTGATGTCAATCATGGTTTGTTGCAGAAAATAAGCTACAAAAATGGGTCCCAAAATGTTGTGGGTGAAGAAATAGCTATGTATTCTAGCTGGGGAAGCGGAGCATACCTTTTTAAACCTGATGGTGATGCGCAGCCTATCATTACAGCAGGTGGGCAGATGGTGATCTCTGAGGGACCTTTGGTGCAGGAAGTATATTCTTATCCAAGTACACAAAGCGAGAAGAGCCCCATTTCTCATAGCACCCGTCTCTACAACGGAGATAATACTGTACAGGAGTTTTTCATTGAGAAGGAATATCATGTTGAGCTCCTCGACCAGCAGTTTAATGACAGGGAGTTGATAGTTAGATACAGAACAGATATTGACAACAAAAAAGTATTCTTTTCTGATTTAAATGGCTTTCAGATGAGCAGGAGAGAAACCTACGATAAAATCCCCCTCCAGGGCAATTACTACCCTATGCCCTCTCTTGCATTCATGCAAGGATCCAATGGTCAGCGGTTTTCTGTCCATTCCCGGCAGTCATTGGGTGTGGCAAGCCTTAAAAATGGATGGTTGGAGATTATGCTTGACCGTCGTTTGGTAAGAGATGACGGACGTGGTCTTGGACAAGGAGTGATGGACAACCGTGCAATGAATGTAATCTTCCACATCCTTGTAGAGTCCAACATTTCTTGTGCATCAAACCCAGTTTCCAACCCATTGCCGTTAAACCCCTCTCTTCTTTCCCACCGTGTCGGTGCTGACCTGAACTATCCATTGCATTCATTCATTTCCAAGAAGCCAGATGATTTGTCAGTGCAACCACCCCCAAGATCATTCTCACCTCTAGCTGCACCCTTACCTTGTGATCTGCATATTGTAAGTTTAAAGGTTCCTCAACCTTTAAAATTCTCAGAGCTACCACTTGAAAATTCGAGGTTTGTCCTAACATTACAGAGACGAAGTTGGGACTCTTCATATTACCGGAAGGGCAGATCTGGTTGCACTAGGTTTGCTGATGAAAGTGTGAATTTGCTCAACATGTTCCGGGAGCTCACTGTATCGAATGCGAGAGCCACTTCATTAAATCTTCTACACGAAGACATAGATATGCTTGGATATACTGAGCAGTTTGGAGATGTTGCTCCAGAAGGGCAAGTCCTCATTTCTCCTATGGAAATACAGGCTTACAAGTTGGAGTTACAGCCACACCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0004572 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006486 GO:0006487 GO:0006491 GO:0006517 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0015923 GO:0015924 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0034645 GO:0036211 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0070085 GO:0071704 GO:0097708 GO:0098588 GO:0098791 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko00513 ko01100 map00510 map00513 map01100

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

914

Amino Acids

104.62

Weight (kDa)

6.03

Isoelectric Point (pI)

42.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	2 - 242	3e-43	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	249 - 351	1e-20	Alpha mannosidase middle domain
Glyco_hydro_38C	PF07748	506 - 715	4.4e-32	Glycosyl hydrolases family 38 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010099)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14950
fragaria_vesca	FvH4_6g49910 FvH4_6g49910
malus_domestica	MD09G1040900.v1.1 MD17G1042200.v1.1
prunus_persica	Prupe.3G276800_v2.0.a1
pyrus_communis	pycom111g03190 pycom17g03770
rosa_chinensis	RchiOBHm_Chr2g0170081
rosa_laevigata	RLG00000021920
rosa_multiflora	Rmu_co8321257.1_g000001 Rmu_sc0013078.1_g000007 Rmu_sc0013453.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081600
rosa_rugosa	Rorug02G0545500 Rorug02G0545600.1
rosa_samantha	Rh2AG617700 Rh2BG629200 Rh2CG598600 Rh2DG641000
rosa_wichuraiana	Rw2G051140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1682
Acc16I	TGCGCA	1 cut(s)	1674
Acc36I	ACCTGC	1 cut(s)	1682
AccB7I	CCANNNNNTGG	1 cut(s)	1177
AccBSI	CCGCTC	1 cut(s)	409
AccI	GTMKAC	1 cut(s)	1344
AccII	CGCG	1 cut(s)	337
AciI	CCGC	6 cut(s)	403, 409, 496, 1168, 1641, 2044
AclWI	GGATC	4 cut(s)	12, 71, 2025, 2038
AcsI	RAATTY	7 cut(s)	22, 61, 190, 919, 2435, 2458, 2557
AcuI	CTGAAG	1 cut(s)	540
AcyI	GRCGYC	1 cut(s)	208
AfaI	GTAC	8 cut(s)	334, 455, 798, 817, 851, 958, 1753, 1815
AfiI	CCNNNNNNNGG	5 cut(s)	1177, 1370, 1987, 1988, 2125
AflII	CTTAAG	1 cut(s)	164
AflIII	ACRYGT	3 cut(s)	335, 1271, 2568
AgsI	TTSAA	4 cut(s)	781, 1046, 1264, 2456
AjiI	CACGTC	1 cut(s)	2144
AjnI	CCWGG	3 cut(s)	661, 978, 1986
AjuI	GAANNNNNNNTTGG	2 cut(s)	1038, 1070
Alw21I	GWGCWC	2 cut(s)	1854, 2583
Alw26I	GTCTC	4 cut(s)	363, 377, 1796, 2479
AlwI	GGATC	4 cut(s)	12, 71, 2025, 2038
AoxI	GGCC	2 cut(s)	293, 770
ApeKI	GCWGC	5 cut(s)	905, 908, 1303, 1675, 2378
ApoI	RAATTY	7 cut(s)	22, 61, 190, 919, 2435, 2458, 2557
AspLEI	GCGC	3 cut(s)	117, 592, 1675
AspS9I	GGNCC	5 cut(s)	150, 293, 1180, 1590, 1718
AsuC2I	CCSGG	2 cut(s)	2062, 2576
AsuHPI	GGTGA	7 cut(s)	84, 722, 1120, 1619, 1679, 1717, 2361
AsuII	TTCGAA	3 cut(s)	129, 619, 1490
AvaII	GGWCC	4 cut(s)	150, 1180, 1590, 1718
BaeGI	GKGCMC	1 cut(s)	415
BamHI	GGATCC	1 cut(s)	2030
BanII	GRGCYC	3 cut(s)	1772, 1854, 2583
BauI	CACGAG	1 cut(s)	1155
BbsI	GAAGAC	2 cut(s)	615, 2634
Bbv12I	GWGCWC	2 cut(s)	1854, 2583
BbvCI	CCTCAGC	1 cut(s)	585
BbvI	GCAGC	5 cut(s)	895, 917, 1315, 1687, 2365
BccI	CCATC	8 cut(s)	381, 826, 938, 1216, 1658, 1696, 2091, 2158
BceAI	ACGGC	2 cut(s)	102, 2236
BcgI	CGANNNNNNTGC	2 cut(s)	794, 828
BciT130I	CCWGG	3 cut(s)	663, 980, 1988
BcnI	CCSGG	2 cut(s)	2062, 2576
BcoDI	GTCTC	4 cut(s)	363, 377, 1796, 2479
BfaI	CTAG	7 cut(s)	425, 800, 912, 1455, 1629, 2375, 2535
BfrI	CTTAAG	1 cut(s)	164
BfuAI	ACCTGC	1 cut(s)	1682
BglII	AGATCT	2 cut(s)	1112, 2522
BisI	GCNGC	5 cut(s)	906, 909, 1304, 1676, 2379
BlsI	GCNGC	5 cut(s)	907, 910, 1305, 1677, 2380
BmcAI	AGTACT	1 cut(s)	798
Bme1390I	CCNGG	5 cut(s)	663, 980, 1988, 2062, 2576
Bme18I	GGWCC	4 cut(s)	150, 1180, 1590, 1718
BmgBI	CACGTC	1 cut(s)	2144
BmgT120I	GGNCC	5 cut(s)	150, 293, 1180, 1590, 1718
BmiI	GGNNCC	8 cut(s)	152, 676, 1165, 1591, 1592, 1719, 2032, 2421
BmrFI	CCNGG	5 cut(s)	663, 980, 1988, 2062, 2576
BmrI	ACTGGG	2 cut(s)	567, 2228
BmsI	GCATC	7 cut(s)	246, 946, 987, 1345, 1537, 1660, 2234
BmuI	ACTGGG	2 cut(s)	567, 2228
BoxI	GACNNNNGTC	1 cut(s)	1274
BpiI	GAAGAC	2 cut(s)	615, 2634
BpmI	CTGGAG	2 cut(s)	1970, 2658
Bpu10I	CCTNAGC	1 cut(s)	585
Bpu14I	TTCGAA	3 cut(s)	129, 619, 1490
BpuMI	CCSGG	2 cut(s)	2062, 2576
BsaBI	GATNNNNATC	2 cut(s)	1551, 1706
BsaHI	GRCGYC	1 cut(s)	208
BsaI	GGTCTC	1 cut(s)	363
BsaJI	CCNNGG	8 cut(s)	42, 99, 362, 662, 819, 882, 1194, 1987
BsaWI	WCCGGW	2 cut(s)	677, 2511
Bsc4I	CCNNNNNNNGG	5 cut(s)	1177, 1370, 1987, 1988, 2125
Bse1I	ACTGG	7 cut(s)	377, 573, 958, 1271, 1333, 1352, 2234
Bse3DI	GCAATG	3 cut(s)	2184, 2246, 2303
Bse8I	GATNNNNATC	2 cut(s)	1551, 1706
BseBI	CCWGG	3 cut(s)	663, 980, 1988
BseDI	CCNNGG	8 cut(s)	42, 99, 362, 662, 819, 882, 1194, 1987
BseGI	GGATG	6 cut(s)	226, 373, 1002, 1415, 2102, 2196
BseJI	GATNNNNATC	2 cut(s)	1551, 1706
BseLI	CCNNNNNNNGG	5 cut(s)	1177, 1370, 1987, 1988, 2125
BseMI	GCAATG	3 cut(s)	2184, 2246, 2303
BseMII	CTCAG	6 cut(s)	539, 576, 1103, 1704, 2454, 2643
BseNI	ACTGG	7 cut(s)	377, 573, 958, 1271, 1333, 1352, 2234
BseRI	GAGGAG	2 cut(s)	647, 1844
BseSI	GKGCMC	1 cut(s)	415
BseXI	GCAGC	5 cut(s)	895, 917, 1315, 1687, 2365
BseYI	CCCAGC	2 cut(s)	216, 1632
BsgI	GTGCAG	3 cut(s)	167, 1748, 2364
Bsh1236I	CGCG	1 cut(s)	337
BshFI	GGCC	2 cut(s)	295, 772
BsiHKAI	GWGCWC	2 cut(s)	1854, 2583
BsiSI	CCGG	4 cut(s)	678, 2062, 2512, 2575
BsiWI	CGTACG	1 cut(s)	332
BslFI	GGGAC	6 cut(s)	1003, 1224, 1262, 1576, 1731, 2510
BslI	CCNNNNNNNGG	5 cut(s)	1177, 1370, 1987, 1988, 2125
BsmAI	GTCTC	4 cut(s)	363, 377, 1796, 2479
BsmBI	CGTCTC	2 cut(s)	1796, 2479
BsmFI	GGGAC	6 cut(s)	1003, 1224, 1262, 1576, 1731, 2510
BsmI	GAATGC	3 cut(s)	2018, 2308, 2599
BsnI	GGCC	2 cut(s)	295, 772
Bso31I	GGTCTC	1 cut(s)	363
Bsp119I	TTCGAA	3 cut(s)	129, 619, 1490
Bsp1286I	GDGCHC	4 cut(s)	415, 1772, 1854, 2583
Bsp1407I	TGTACA	2 cut(s)	453, 1813
Bsp19I	CCATGG	1 cut(s)	99
BspACI	CCGC	6 cut(s)	403, 409, 496, 1168, 1641, 2044
BspANI	GGCC	2 cut(s)	295, 772
BspCNI	CTCAG	6 cut(s)	538, 577, 1102, 1705, 2453, 2644
BspFNI	CGCG	1 cut(s)	337
BspHI	TCATGA	1 cut(s)	940
BspLI	GGNNCC	8 cut(s)	152, 676, 1165, 1591, 1592, 1719, 2032, 2421
BspMI	ACCTGC	1 cut(s)	1682
BspPI	GGATC	4 cut(s)	12, 71, 2025, 2038
BspQI	GCTCTTC	1 cut(s)	1760
BspT104I	TTCGAA	3 cut(s)	129, 619, 1490
BspTI	CTTAAG	1 cut(s)	164
BspTNI	GGTCTC	1 cut(s)	363
BsrBI	CCGCTC	1 cut(s)	409
BsrDI	GCAATG	3 cut(s)	2184, 2246, 2303
BsrGI	TGTACA	2 cut(s)	453, 1813
BsrI	ACTGG	7 cut(s)	377, 573, 958, 1271, 1333, 1352, 2234
BssECI	CCNNGG	8 cut(s)	42, 99, 362, 662, 819, 882, 1194, 1987
BssNI	GRCGYC	1 cut(s)	208
BssSI	CACGAG	1 cut(s)	1155
BssT1I	CCWWGG	4 cut(s)	99, 362, 882, 1194
Bst2BI	CACGAG	1 cut(s)	1155
Bst2UI	CCWGG	3 cut(s)	663, 980, 1988
Bst6I	CTCTTC	4 cut(s)	1206, 1760, 2271, 2506
BstACI	GRCGYC	1 cut(s)	208
BstAFI	CTTAAG	1 cut(s)	164
BstAPI	GCANNNNNTGC	1 cut(s)	1018
BstAUI	TGTACA	2 cut(s)	453, 1813
BstBI	TTCGAA	3 cut(s)	129, 619, 1490
BstC8I	GCNNGC	4 cut(s)	303, 411, 1353, 2083
BstDEI	CTNAG	8 cut(s)	57, 525, 585, 1089, 1436, 1713, 2440, 2652
BstDSI	CCRYGG	3 cut(s)	42, 99, 819
BstF5I	GGATG	6 cut(s)	226, 373, 1002, 1415, 2102, 2196
BstFNI	CGCG	1 cut(s)	337
BstHHI	GCGC	3 cut(s)	117, 592, 1675
BstMAI	GTCTC	4 cut(s)	363, 377, 1796, 2479
BstMWI	GCNNNNNNNGC	8 cut(s)	504, 1018, 1151, 1300, 1427, 1508, 1638, 1767
BstNI	CCWGG	3 cut(s)	663, 980, 1988
BstNSI	RCATGY	2 cut(s)	1275, 2572
BstPAI	GACNNNNGTC	1 cut(s)	1274
BstSCI	CCNGG	5 cut(s)	661, 978, 1986, 2060, 2574
BstSLI	GKGCMC	1 cut(s)	415
BstUI	CGCG	1 cut(s)	337
BstV1I	GCAGC	5 cut(s)	895, 917, 1315, 1687, 2365
BstV2I	GAAGAC	2 cut(s)	615, 2634
BstX2I	RGATCY	4 cut(s)	76, 1112, 2030, 2522
BstYI	RGATCY	4 cut(s)	76, 1112, 2030, 2522
BsuRI	GGCC	2 cut(s)	295, 772
BtgI	CCRYGG	3 cut(s)	42, 99, 819
BtrI	CACGTC	1 cut(s)	2144
BtsCI	GGATG	6 cut(s)	226, 373, 1002, 1415, 2102, 2196
BtsI	GCAGTG	1 cut(s)	179
BtsIMutI	CAGTG	6 cut(s)	179, 449, 1182, 1326, 2349, 2583
BveI	ACCTGC	1 cut(s)	1682
Cac8I	GCNNGC	4 cut(s)	303, 411, 1353, 2083
CciI	TCATGA	1 cut(s)	940
CfoI	GCGC	3 cut(s)	117, 592, 1675
Cfr13I	GGNCC	5 cut(s)	150, 293, 1180, 1590, 1718
CseI	GACGC	1 cut(s)	197
Csp6I	GTAC	8 cut(s)	333, 454, 797, 816, 850, 957, 1752, 1814
CviQI	GTAC	8 cut(s)	333, 454, 797, 816, 850, 957, 1752, 1814
DdeI	CTNAG	8 cut(s)	57, 525, 585, 1089, 1436, 1713, 2440, 2652
DraI	TTTAAA	5 cut(s)	579, 1657, 1938, 2415, 2433
Eam1104I	CTCTTC	4 cut(s)	1206, 1760, 2271, 2506
EarI	CTCTTC	4 cut(s)	1206, 1760, 2271, 2506
Ecl136II	GAGCTC	2 cut(s)	1852, 2581
Eco130I	CCWWGG	4 cut(s)	99, 362, 882, 1194
Eco147I	AGGCCT	1 cut(s)	772
Eco24I	GRGCYC	3 cut(s)	1772, 1854, 2583
Eco31I	GGTCTC	1 cut(s)	363
Eco47I	GGWCC	4 cut(s)	150, 1180, 1590, 1718
Eco53kI	GAGCTC	2 cut(s)	1852, 2581
Eco57I	CTGAAG	1 cut(s)	540
EcoICRI	GAGCTC	2 cut(s)	1852, 2581
EcoO109I	RGGNCCY	3 cut(s)	150, 1590, 1718
EcoRI	GAATTC	1 cut(s)	61
EcoRII	CCWGG	3 cut(s)	661, 978, 1986
EcoT14I	CCWWGG	4 cut(s)	99, 362, 882, 1194
EcoT38I	GRGCYC	3 cut(s)	1772, 1854, 2583
ErhI	CCWWGG	4 cut(s)	99, 362, 882, 1194
Esp3I	CGTCTC	2 cut(s)	1796, 2479
FalI	AAGNNNNNCTT	4 cut(s)	866, 898, 1038, 1070
FaqI	GGGAC	6 cut(s)	1003, 1224, 1262, 1576, 1731, 2510
FauI	CCCGC	3 cut(s)	396, 402, 1175
FblI	GTMKAC	1 cut(s)	1344
Fnu4HI	GCNGC	5 cut(s)	906, 909, 1304, 1676, 2379
FokI	GGATG	6 cut(s)	233, 360, 1009, 1422, 2109, 2183
FriOI	GRGCYC	3 cut(s)	1772, 1854, 2583
Fsp4HI	GCNGC	5 cut(s)	906, 909, 1304, 1676, 2379
FspBI	CTAG	7 cut(s)	425, 800, 912, 1455, 1629, 2375, 2535
FspI	TGCGCA	1 cut(s)	1674
GlaI	GCGC	3 cut(s)	116, 591, 1674
GluI	GCNGC	5 cut(s)	906, 909, 1304, 1676, 2379
GsaI	CCCAGC	2 cut(s)	220, 1636
GsuI	CTGGAG	2 cut(s)	1970, 2658
HaeIII	GGCC	2 cut(s)	295, 772
HapII	CCGG	4 cut(s)	678, 2062, 2512, 2575
HgaI	GACGC	1 cut(s)	197
HhaI	GCGC	3 cut(s)	117, 592, 1675
Hin1I	GRCGYC	1 cut(s)	208
Hin6I	GCGC	3 cut(s)	115, 590, 1673
HinP1I	GCGC	3 cut(s)	115, 590, 1673
HincII	GTYRAC	1 cut(s)	1234
HindII	GTYRAC	1 cut(s)	1234
HinfI	GANTC	4 cut(s)	230, 991, 2207, 2498
HpaI	GTTAAC	1 cut(s)	1234
HpaII	CCGG	4 cut(s)	678, 2062, 2512, 2575
HphI	GGTGA	7 cut(s)	84, 722, 1120, 1619, 1679, 1717, 2361
Hpy166II	GTNNAC	4 cut(s)	250, 1234, 1345, 1754
Hpy188I	TCNGA	9 cut(s)	9, 147, 213, 996, 1257, 1714, 1933, 1950, 2443
Hpy188III	TCNNGA	6 cut(s)	629, 941, 1157, 1241, 1519, 2675
Hpy8I	GTNNAC	4 cut(s)	250, 1234, 1345, 1754
Hpy99I	CGWCG	1 cut(s)	2124
HpyAV	CCTTC	9 cut(s)	11, 708, 909, 1095, 1154, 1326, 1828, 2509, 2672
HpyCH4IV	ACGT	2 cut(s)	501, 2143
HpyF10VI	GCNNNNNNNGC	8 cut(s)	504, 1018, 1151, 1300, 1427, 1508, 1638, 1767
HpyF3I	CTNAG	8 cut(s)	57, 525, 585, 1089, 1436, 1713, 2440, 2652
HpySE526I	ACGT	2 cut(s)	501, 2143
Hsp92I	GRCGYC	1 cut(s)	208
HspAI	GCGC	3 cut(s)	115, 590, 1673
KflI	GGGWCCC	1 cut(s)	1590
KspAI	GTTAAC	1 cut(s)	1234
LguI	GCTCTTC	1 cut(s)	1760
LmnI	GCTCC	6 cut(s)	406, 1198, 1643, 1857, 2578, 2677
Lsp1109I	GCAGC	5 cut(s)	895, 917, 1315, 1687, 2365
LweI	GCATC	7 cut(s)	246, 946, 987, 1345, 1537, 1660, 2234
MaeI	CTAG	7 cut(s)	425, 800, 912, 1455, 1629, 2375, 2535
MaeII	ACGT	2 cut(s)	501, 2143
MaeIII	GTNAC	6 cut(s)	86, 710, 876, 949, 1246, 2727
MbiI	CCGCTC	1 cut(s)	409
MflI	RGATCY	4 cut(s)	76, 1112, 2030, 2522
MhlI	GDGCHC	4 cut(s)	415, 1772, 1854, 2583
MluI	ACGCGT	1 cut(s)	335
MlyI	GAGTC	3 cut(s)	985, 2216, 2492
MmeI	TCCRAC	6 cut(s)	667, 1387, 2082, 2235, 2265, 2703
MslI	CAYNNNNRTG	6 cut(s)	288, 824, 942, 945, 2636, 2740
MspA1I	CMGCKG	2 cut(s)	908, 2044
MspCI	CTTAAG	1 cut(s)	164
MspI	CCGG	4 cut(s)	678, 2062, 2512, 2575
MspR9I	CCNGG	5 cut(s)	663, 980, 1988, 2062, 2576
Mva1269I	GAATGC	3 cut(s)	2018, 2308, 2599
MvaI	CCWGG	3 cut(s)	663, 980, 1988
MvnI	CGCG	1 cut(s)	337
MwoI	GCNNNNNNNGC	8 cut(s)	504, 1018, 1151, 1300, 1427, 1508, 1638, 1767
NciI	CCSGG	2 cut(s)	2062, 2576
NcoI	CCATGG	1 cut(s)	99
NlaIV	GGNNCC	8 cut(s)	152, 676, 1165, 1591, 1592, 1719, 2032, 2421
NmuCI	GTSAC	1 cut(s)	710
NsbI	TGCGCA	1 cut(s)	1674
NspI	RCATGY	2 cut(s)	1275, 2572
NspV	TTCGAA	3 cut(s)	129, 619, 1490
PagI	TCATGA	1 cut(s)	940
PaqCI	CACCTGC	1 cut(s)	1682
PceI	AGGCCT	1 cut(s)	772
PciI	ACATGT	2 cut(s)	1271, 2568
PciSI	GCTCTTC	1 cut(s)	1760
PcsI	WCGNNNNNNNCGW	1 cut(s)	625
PctI	GAATGC	3 cut(s)	2018, 2308, 2599
PfeI	GAWTC	1 cut(s)	230
Pfl23II	CGTACG	1 cut(s)	332
PflFI	GACNNNGTC	1 cut(s)	2145
PflMI	CCANNNNNTGG	1 cut(s)	1177
PfoI	TCCNGGA	2 cut(s)	978, 2574
PkrI	GCNGC	5 cut(s)	907, 910, 1305, 1677, 2380
PleI	GAGTC	3 cut(s)	985, 2215, 2492
PpsI	GAGTC	3 cut(s)	985, 2215, 2492
PpuMI	RGGWCCY	3 cut(s)	150, 1590, 1718
PscI	ACATGT	2 cut(s)	1271, 2568
PshAI	GACNNNNGTC	1 cut(s)	1274
Psp124BI	GAGCTC	2 cut(s)	1854, 2583
Psp5II	RGGWCCY	3 cut(s)	150, 1590, 1718
Psp6I	CCWGG	3 cut(s)	661, 978, 1986
PspFI	CCCAGC	2 cut(s)	216, 1632
PspGI	CCWGG	3 cut(s)	661, 978, 1986
PspLI	CGTACG	1 cut(s)	332
PspN4I	GGNNCC	8 cut(s)	152, 676, 1165, 1591, 1592, 1719, 2032, 2421
PspPI	GGNCC	5 cut(s)	150, 293, 1180, 1590, 1718
PspPPI	RGGWCCY	3 cut(s)	150, 1590, 1718
PsuI	RGATCY	4 cut(s)	76, 1112, 2030, 2522
PsyI	GACNNNGTC	1 cut(s)	2145
PvuII	CAGCTG	1 cut(s)	908
RsaI	GTAC	8 cut(s)	334, 455, 798, 817, 851, 958, 1753, 1815
RsaNI	GTAC	8 cut(s)	333, 454, 797, 816, 850, 957, 1752, 1814
RseI	CAYNNNNRTG	6 cut(s)	288, 824, 942, 945, 2636, 2740
SacI	GAGCTC	2 cut(s)	1854, 2583
SapI	GCTCTTC	1 cut(s)	1760
SatI	GCNGC	5 cut(s)	906, 909, 1304, 1676, 2379
Sau96I	GGNCC	5 cut(s)	150, 293, 1180, 1590, 1718
ScaI	AGTACT	1 cut(s)	798
SchI	GAGTC	3 cut(s)	985, 2216, 2492
ScrFI	CCNGG	5 cut(s)	663, 980, 1988, 2062, 2576
SduI	GDGCHC	4 cut(s)	415, 1772, 1854, 2583
SfaNI	GCATC	7 cut(s)	246, 946, 987, 1345, 1537, 1660, 2234
SfuI	TTCGAA	3 cut(s)	129, 619, 1490
SinI	GGWCC	4 cut(s)	150, 1180, 1590, 1718
SmiI	ATTTAAAT	1 cut(s)	1938
SmiMI	CAYNNNNRTG	6 cut(s)	288, 824, 942, 945, 2636, 2740
SmlI	CTYRAG	1 cut(s)	164
SmoI	CTYRAG	1 cut(s)	164
SseBI	AGGCCT	1 cut(s)	772
SsiI	CCGC	6 cut(s)	403, 409, 496, 1168, 1641, 2044
SspMI	CTAG	7 cut(s)	425, 800, 912, 1455, 1629, 2375, 2535
SstI	GAGCTC	2 cut(s)	1854, 2583
StuI	AGGCCT	1 cut(s)	772
StyD4I	CCNGG	5 cut(s)	661, 978, 1986, 2060, 2574
StyI	CCWWGG	4 cut(s)	99, 362, 882, 1194
SwaI	ATTTAAAT	1 cut(s)	1938
TaiI	ACGT	2 cut(s)	504, 2146
TaqI	TCGA	8 cut(s)	129, 321, 619, 793, 1490, 1857, 2462, 2591
TatI	WGTACW	4 cut(s)	453, 796, 1751, 1813
TfiI	GAWTC	1 cut(s)	230
TscAI	CASTG	6 cut(s)	186, 456, 1182, 1333, 2349, 2590
TseFI	GTSAC	1 cut(s)	710
TseI	GCWGC	5 cut(s)	905, 908, 1303, 1675, 2378
Tsp45I	GTSAC	1 cut(s)	710
TspGWI	ACGGA	3 cut(s)	836, 1815, 2154
TspRI	CASTG	6 cut(s)	186, 456, 1182, 1333, 2349, 2590
Tth111I	GACNNNGTC	1 cut(s)	2145
Van91I	CCANNNNNTGG	1 cut(s)	1177
Vha464I	CTTAAG	1 cut(s)	164
VpaK11BI	GGWCC	4 cut(s)	150, 1180, 1590, 1718
XapI	RAATTY	7 cut(s)	22, 61, 190, 919, 2435, 2458, 2557
XceI	RCATGY	2 cut(s)	1275, 2572
XmiI	GTMKAC	1 cut(s)	1344
XspI	CTAG	7 cut(s)	425, 800, 912, 1455, 1629, 2375, 2535
ZrmI	AGTACT	1 cut(s)	798

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.