Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010099

Physical Location & Seq

Genomic Coordinates Forward (+)

2634335 .. 2639232

Gene Structure 4898 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1040900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3486 bp

ATGGCTTTCTCATCGTACATCGGCAGCACCCGCCGCGGCGGTTGGGCCAACTCCCTCCTCCCCTCCTCCTCCGCTTCCAACCCCAAATCCAAGCTCACCAGAAAGCCCCGCAGGCGATTATCCCTCCGCGACTTCATCTTCGCAAACTTCTTCATCATCGGCCTCTCGATCTCCCTCTTCCTATTTCTCATCGTCATCCTCCGCTACGGCGTCCCCACACCCCTCTCCACCCACTTCAAATCCAAATCCTCCACCCGCTTCTCCAAGCCCCGAAAGCCCGTCTCCCGCAAACCCGATTCGGGCGCCGACGCCGCTGCGGGGGCCACCGTTGACATCACCACCAAGGAGCTGTACGACAAGATTGAATTCTCGGATGTGGACGGCGGGCCGTGGAAGCAGGGGTGGCGGGTGAGCTACAAAGGGGACGAGTGGGACTCTGAGAAATTGAAGGTGTTTGTGGTGCCCCATTCGCATAACGATCCCGGCTGGAAGCTCACCGTCGAAGAGTACTACGATAGGCAATCCAGGCATATTCTCGACACCATTGTCGATACGCTTTCTAAGGATTCTCGGCGCAAGTTTATATGGGAAGAGATGTCTTATCTGGAAAGATGGTGGAGGGACTCCTCTGATCACAAAAGGGAGTCATTCACAAATTTGGTGAAGAATGGTCAGCTGGAAATTGTTGGAGGTGGCTGGGTAATGAACGATGAGGCTAATTCACATTATTATGCCATAATTGAGCAGATGACTGAAGGAAATATGTGGTTGAATGAAACTGTTGGGGTTGTTCCTAAAAATGCATGGGCAATAGATCCGTTTGGATACTCACCTACCATGGCATATCTTCTCCGTCGCATGGGTTTCGAAAATATGCTTATTCAGAGGACCCATTATGAGCTGAAGAAGGAACTTGCACTGCATAAAAATTTGGAGTATATATGGCGTCAGAGCTGGGATGTGGATGAAACTACTGATATTTTTGTCCATATGATGCCCTTTTATTCTTATGATATTCCACATACTTGTGGGCCAGAGCCTGCCATTTGTTGTCAGTTTGACTTTGCTCGAATGCGTGGCTTTGTTTATGAACTTTGTCCGTGGGGAGACAATCCAGTAGAGACCAACCAGGAAAATGTTCAGGAGCGGGCACTTATTCTACTGGATCAATATAAGAAGAAATCAACACTGTACAGGACAAATACACTTCTTATTCCTCTTGGAGATGATTTCCGCTACATAAGCATCGATGAAGCTGAAGCTCAGTTTAGGAACTACCAAATGTTGTTTGACTATATCAATTCTAATCCCAGTTTAAACACAGAGGTCAAGTTTGGAACTTTGGAAGATTACTTTTCGACCCTTCGTGAGGAGGCTGAAAGAATAAATCATTCACTTCCTGGTGAGATTGGTTCTGGTCAGGTTGGGGGTTTTCCTTCTTTGTCAGGTGACTTCTTCACTTATGCTGATAGGCAACAGGACTATTGGAGTGGCTATTATGTGTCCAGGCCTTTCTTCAAGGCTGTTGATCGAGTACTCGAGCAAACACTTCGTACCACAGATATGATGATGGCTTTCCTGCTTGGGTACTGCGAGAGAGCACAATGTGAAAAGTTGCCCATGGGGTTCTCCTACAAGTTGGCTGCTGCCAGGAGGAACTTGGCTCTTTTCCAGCATCATGATGGTGTGACTGGTACTGCTAAAGATCATGTGGTCCTGGACTATGGGACTCGGATGCACACATCTTTGCAGGACTTGCAGATTTTCATGTCTAAAGCTATTGAAGTACTGCTTGGAATACGCCATGAGAAAAATGACAATAACCCTTCTCAGTTTGAGCCAGAACAGGTGAGATCTAAATATGATGTTCAGCCTGTCCATAGAGCAATCATGGCCCGTGAAGGAACTAGACAATCAGTGGTGTTTTTTAATCCTTTGGAGCAGACAAGAGAAGAGGTTGTGATGGTTATTGTTAACCGTCCAGATGTTACTGTTCTGGACTCAAACTCGACATGTGTTCAAAGCCAAATATCTCCTGAGCTGCAGCATGATAAAAGCAAAATATTTACTGGAAGGCATCGTGTCTACTGGCAGGTTTCTGTTCCCGCCTTGGGGTTGCAAACATATTACATTGCTAACGGGTTGCATGGATGTGAAAAGGCCAAACCAGCAAAACTAAGATTCTTCTCAAAGTCTAGTTCTTTATCTTGCCCCACTCCATATGCATGCTCAAAAGCAGATGCTGATGTGGCTGAAATCCAGAACAGGCACCAAATACTCACCTTTGATGTGAAGCATGGTCTGTTGCAGAAAGTCAGCTATAAGAATGGTTCCCCAAATGTTGTGGGTGAAGAAATAGATATGTACTCTAGTTGGGGAAGTGGAGCCTACCTATTTAAACCCAACGGTGATGCACAGGTTATCATCGAAGAAGGTGGGCAGTTGGTGATCTCTGAGGGCCCTTTGGTGCAGGAAGTATACTCTTATCCAAGGACTGAATGGGAAAAGAGCCCCATTTCTCATAGCACTCGTGTCTACAATGGAGAGAATACTGTACAGGAATTTCTCATCGAGAAGGAGTATCATGTTGAGCTTCTTGGTCAGGAGTTCGATGACAAGGAGTTGATAGTTAGATATAAAACAGATGTTGATAACAAAAGAATATTCTTTTCTGATTTAAATGGCTTTCAGATGAGCCGGAGAGAAACCTATGATAAAATCCCAGTTCAGGGCAATTACTACCCTATGCCTTCTCTTGCATTCATGCAGGGGTCTGCTGGTCAGCGGTTTTCAGTCCATTCCCGGCAGTCTTTGGGTGTGGCAAGTCTTAAAAATGGATGGTTGGAGATTATGCTTGACCGTCGTTTGGTAAGAGATGATGGACGAGGTCTTGGACAAGGAGTGATGGACAATCGTGCAATGAATGTAATCTTCCACATCGTTGTGGAGTCTAACATTTCAGCCACATCAAACCCGGTTTCCAACCCATTGCCCTTAAATCCCTCGCTTCTTTCTCACTGCGTCAGTGCTCACTTGAACTATCCGTTGCATGCATTCATTGCCAAGAAGCCAGAGGAGTTGTCAGTGCAGCCACCCCCAAGGTCTTTCTCCCCCTTAGCCGCTCCCTTACCATGTGATCTGCATATTGTAAGTTTTAAGGTTCCCCAACCTCTAAAATACTCTCAGCAACCTCTCGAAGATTCTAGGTTTGCCCTAATTTTACAGAGACAGAACTGGGACGCTTCATATTGTCGGAAGGGCAGATCAGGGTGCACTAGATTTGCTGATGAGACTGTGAATCTCTTTTACATGTTCAAGGACCTTAAGGTATTGAATGCGAGAGTTACTTCCTTAAATCTTTTACATGAGGACATGGATATGCTTGGGTATACTGAGCAATTTGGAGATTTTGCTCAAGATGGACACGTTCTTATCTCTCCAATGGAAATACAGGCTTACAAGTTGGAATTGCGGCCGCCCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0004572 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006486 GO:0006487 GO:0006491 GO:0006517 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0015923 GO:0015924 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0034645 GO:0036211 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0070085 GO:0071704 GO:0097708 GO:0098588 GO:0098791 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko00513 ko01100 map00510 map00513 map01100

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1162

Amino Acids

133.1

Weight (kDa)

6.44

Isoelectric Point (pI)

45.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	150 - 488	2.2e-84	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	495 - 597	2.9e-20	Alpha mannosidase middle domain
Glyco_hydro_38C	PF07748	753 - 962	9.5e-30	Glycosyl hydrolases family 38 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010099)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14950
fragaria_vesca	FvH4_6g49910 FvH4_6g49910
malus_domestica	MD09G1040900.v1.1 MD17G1042200.v1.1
prunus_persica	Prupe.3G276800_v2.0.a1
pyrus_communis	pycom111g03190 pycom17g03770
rosa_chinensis	RchiOBHm_Chr2g0170081
rosa_laevigata	RLG00000021920
rosa_multiflora	Rmu_co8321257.1_g000001 Rmu_sc0013078.1_g000007 Rmu_sc0013453.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081600
rosa_rugosa	Rorug02G0545500 Rorug02G0545600.1
rosa_samantha	Rh2AG617700 Rh2BG629200 Rh2CG598600 Rh2DG641000
rosa_wichuraiana	Rw2G051140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	545
Acc36I	ACCTGC	1 cut(s)	2083
AccB1I	GGYRCC	3 cut(s)	302, 460, 2268
AccBSI	CCGCTC	2 cut(s)	1147, 3122
AccI	GTMKAC	4 cut(s)	2085, 2478, 2535, 3389
AccII	CGCG	2 cut(s)	36, 129
AclWI	GGATC	3 cut(s)	473, 809, 1173
AcoI	YGGCCR	1 cut(s)	3473
AcsI	RAATTY	4 cut(s)	365, 655, 928, 2561
AcuI	CTGAAG	3 cut(s)	774, 923, 1278
AcyI	GRCGYC	4 cut(s)	210, 303, 309, 946
AdeI	CACNNNGTG	1 cut(s)	1607
AfiI	CCNNNNNNNGG	9 cut(s)	299, 318, 859, 1369, 2111, 2112, 2728, 2729, 2866
AflII	CTTAAG	1 cut(s)	3323
AflIII	ACRYGT	3 cut(s)	2012, 3309, 3424
AjnI	CCWGG	6 cut(s)	524, 1128, 1399, 1505, 1649, 1716
AjuI	GAANNNNNNNTTGG	2 cut(s)	1776, 1808
Alw21I	GWGCWC	3 cut(s)	1603, 3031, 3275
Alw26I	GTCTC	5 cut(s)	286, 1101, 1115, 3220, 3284
Alw44I	GTGCAC	1 cut(s)	3271
AlwI	GGATC	3 cut(s)	473, 809, 1173
AlwNI	CAGNNNCTG	2 cut(s)	1040, 2243
Ama87I	CYCGRG	1 cut(s)	1538
ApaI	GGGCCC	1 cut(s)	2462
ApaLI	GTGCAC	1 cut(s)	3271
ApeKI	GCWGC	7 cut(s)	24, 314, 1643, 1646, 2041, 2044, 3088
ApoI	RAATTY	4 cut(s)	365, 655, 928, 2561
ArsI	GACNNNNNNTTYG	2 cut(s)	122, 154
Asp700I	GAANNNNTTC	1 cut(s)	1137
AspLEI	GCGC	2 cut(s)	305, 576
AsuC2I	CCSGG	3 cut(s)	483, 2803, 2975
AsuII	TTCGAA	1 cut(s)	867
AvaI	CYCGRG	1 cut(s)	1538
AvaII	GGWCC	3 cut(s)	888, 1714, 3319
BaeGI	GKGCMC	4 cut(s)	465, 1153, 2462, 3275
BanI	GGYRCC	3 cut(s)	302, 460, 2268
BanII	GRGCYC	2 cut(s)	2462, 2513
BauI	CACGAG	1 cut(s)	2529
Bbv12I	GWGCWC	3 cut(s)	1603, 3031, 3275
BbvI	GCAGC	7 cut(s)	36, 301, 1630, 1633, 2028, 2056, 3100
BccI	CCATC	8 cut(s)	606, 1564, 1676, 1957, 2832, 2873, 2899, 3413
BceAI	ACGGC	3 cut(s)	223, 373, 397
BcgI	CGANNNNNNTGC	6 cut(s)	296, 330, 847, 881, 1532, 1566
BciT130I	CCWGG	6 cut(s)	526, 1130, 1401, 1507, 1651, 1718
BciVI	GTATCC	1 cut(s)	818
BclI	TGATCA	1 cut(s)	631
BcnI	CCSGG	3 cut(s)	483, 2803, 2975
BcoDI	GTCTC	5 cut(s)	286, 1101, 1115, 3220, 3284
BfaI	CTAG	5 cut(s)	1908, 2196, 2370, 3204, 3276
BfmI	CTRYAG	1 cut(s)	2042
BfoI	RGCGCY	1 cut(s)	306
BfrI	CTTAAG	1 cut(s)	3323
BfuAI	ACCTGC	1 cut(s)	2083
BfuI	GTATCC	1 cut(s)	818
BglI	GCCNNNNNGGC	1 cut(s)	112
BglII	AGATCT	1 cut(s)	1853
BmcAI	AGTACT	3 cut(s)	509, 1536, 1788
Bme1390I	CCNGG	9 cut(s)	483, 526, 1130, 1401, 1507, 1651, 1718, 2803, 2975
Bme18I	GGWCC	3 cut(s)	888, 1714, 3319
BmeT110I	CYCGRG	1 cut(s)	1538
BmiI	GGNNCC	9 cut(s)	304, 322, 462, 890, 2270, 2332, 2386, 2460, 3162
BmrFI	CCNGG	9 cut(s)	483, 526, 1130, 1401, 1507, 1651, 1718, 2803, 2975
BmrI	ACTGGG	3 cut(s)	1305, 2717, 3244
BmsI	GCATC	7 cut(s)	984, 1254, 1684, 1725, 2086, 2230, 2401
BmuI	ACTGGG	3 cut(s)	1305, 2717, 3244
BplI	GAGNNNNNCTC	2 cut(s)	419, 451
Bpu10I	CCTNAGC	2 cut(s)	2037, 3115
Bpu14I	TTCGAA	1 cut(s)	867
BpuEI	CTTGAG	1 cut(s)	3399
BpuMI	CCSGG	3 cut(s)	483, 2803, 2975
Bsa29I	ATCGAT	1 cut(s)	1248
BsaHI	GRCGYC	4 cut(s)	210, 303, 309, 946
BsaI	GGTCTC	1 cut(s)	1115
BsaJI	CCNNGG	9 cut(s)	34, 342, 389, 837, 1100, 1620, 2109, 2489, 3098
Bsc4I	CCNNNNNNNGG	9 cut(s)	299, 318, 859, 1369, 2111, 2112, 2728, 2729, 2866
Bse1I	ACTGG	8 cut(s)	1115, 1167, 1311, 1696, 2074, 2093, 2723, 3239
Bse3DI	GCAATG	4 cut(s)	2130, 2925, 2987, 3057
BseBI	CCWGG	6 cut(s)	526, 1130, 1401, 1507, 1651, 1718
BseCI	ATCGAT	1 cut(s)	1248
BseDI	CCNNGG	9 cut(s)	34, 342, 389, 837, 1100, 1620, 2109, 2489, 3098
BseGI	GGATG	7 cut(s)	195, 379, 964, 970, 1740, 2156, 2843
BseLI	CCNNNNNNNGG	9 cut(s)	299, 318, 859, 1369, 2111, 2112, 2728, 2729, 2866
BseMI	GCAATG	4 cut(s)	2130, 2925, 2987, 3057
BseMII	CTCAG	7 cut(s)	429, 1277, 1844, 2028, 2445, 3197, 3384
BseNI	ACTGG	8 cut(s)	1115, 1167, 1311, 1696, 2074, 2093, 2723, 3239
BseRI	GAGGAG	6 cut(s)	47, 55, 58, 616, 1385, 3089
BseSI	GKGCMC	4 cut(s)	465, 1153, 2462, 3275
BseX3I	CGGCCG	1 cut(s)	3473
BseXI	GCAGC	7 cut(s)	36, 301, 1630, 1633, 2028, 2056, 3100
BseYI	CCCAGC	2 cut(s)	696, 954
BsgI	GTGCAG	2 cut(s)	2489, 3107
Bsh1236I	CGCG	2 cut(s)	36, 129
Bsh1285I	CGRYCG	1 cut(s)	3476
BshNI	GGYRCC	3 cut(s)	302, 460, 2268
BshVI	ATCGAT	1 cut(s)	1248
BsiEI	CGRYCG	1 cut(s)	3476
BsiHKAI	GWGCWC	3 cut(s)	1603, 3031, 3275
BsiHKCI	CYCGRG	1 cut(s)	1538
BsiSI	CCGG	4 cut(s)	483, 2698, 2803, 2975
BslFI	GGGAC	6 cut(s)	197, 437, 446, 635, 1741, 3251
BslI	CCNNNNNNNGG	9 cut(s)	299, 318, 859, 1369, 2111, 2112, 2728, 2729, 2866
BsmAI	GTCTC	5 cut(s)	286, 1101, 1115, 3220, 3284
BsmBI	CGTCTC	1 cut(s)	286
BsmFI	GGGAC	6 cut(s)	197, 437, 446, 635, 1741, 3251
BsmI	GAATGC	4 cut(s)	1077, 2759, 3053, 3340
Bso31I	GGTCTC	1 cut(s)	1115
BsoBI	CYCGRG	1 cut(s)	1538
Bsp119I	TTCGAA	1 cut(s)	867
Bsp120I	GGGCCC	1 cut(s)	2458
Bsp1286I	GDGCHC	7 cut(s)	465, 1153, 1603, 2462, 2513, 3031, 3275
Bsp1407I	TGTACA	2 cut(s)	1191, 2554
Bsp19I	CCATGG	2 cut(s)	837, 1620
BspCNI	CTCAG	7 cut(s)	430, 1276, 1843, 2029, 2446, 3196, 3385
BspDI	ATCGAT	1 cut(s)	1248
BspFNI	CGCG	2 cut(s)	36, 129
BspHI	TCATGA	1 cut(s)	1678
BspLI	GGNNCC	9 cut(s)	304, 322, 462, 890, 2270, 2332, 2386, 2460, 3162
BspMAI	CTGCAG	1 cut(s)	2046
BspMI	ACCTGC	1 cut(s)	2083
BspPI	GGATC	3 cut(s)	473, 809, 1173
BspT104I	TTCGAA	1 cut(s)	867
BspT107I	GGYRCC	3 cut(s)	302, 460, 2268
BspTI	CTTAAG	1 cut(s)	3323
BspTNI	GGTCTC	1 cut(s)	1115
BsrBI	CCGCTC	2 cut(s)	1147, 3122
BsrDI	GCAATG	4 cut(s)	2130, 2925, 2987, 3057
BsrGI	TGTACA	2 cut(s)	1191, 2554
BsrI	ACTGG	8 cut(s)	1115, 1167, 1311, 1696, 2074, 2093, 2723, 3239
BssECI	CCNNGG	9 cut(s)	34, 342, 389, 837, 1100, 1620, 2109, 2489, 3098
BssNAI	GTATAC	2 cut(s)	2479, 3390
BssNI	GRCGYC	4 cut(s)	210, 303, 309, 946
BssSI	CACGAG	1 cut(s)	2529
BssT1I	CCWWGG	6 cut(s)	342, 837, 1620, 2109, 2489, 3098
Bst1107I	GTATAC	2 cut(s)	2479, 3390
Bst2BI	CACGAG	1 cut(s)	2529
Bst2UI	CCWGG	6 cut(s)	526, 1130, 1401, 1507, 1651, 1718
Bst6I	CTCTTC	4 cut(s)	182, 498, 585, 1947
BstACI	GRCGYC	4 cut(s)	210, 303, 309, 946
BstAFI	CTTAAG	1 cut(s)	3323
BstAPI	GCANNNNNTGC	2 cut(s)	1756, 3059
BstAUI	TGTACA	2 cut(s)	1191, 2554
BstBI	TTCGAA	1 cut(s)	867
BstC8I	GCNNGC	6 cut(s)	113, 386, 1041, 1149, 2227, 3051
BstDSI	CCRYGG	5 cut(s)	34, 389, 837, 1100, 1620
BstENI	CCTNNNNNAGG	1 cut(s)	1367
BstF5I	GGATG	7 cut(s)	195, 379, 964, 970, 1740, 2156, 2843
BstFNI	CGCG	2 cut(s)	36, 129
BstH2I	RGCGCY	1 cut(s)	306
BstHHI	GCGC	2 cut(s)	305, 576
BstMAI	GTCTC	5 cut(s)	286, 1101, 1115, 3220, 3284
BstMCI	CGRYCG	1 cut(s)	3476
BstNI	CCWGG	6 cut(s)	526, 1130, 1401, 1507, 1651, 1718
BstNSI	RCATGY	4 cut(s)	2016, 2229, 3053, 3313
BstSCI	CCNGG	9 cut(s)	481, 524, 1128, 1399, 1505, 1649, 1716, 2801, 2973
BstSFI	CTRYAG	1 cut(s)	2042
BstSLI	GKGCMC	4 cut(s)	465, 1153, 2462, 3275
BstUI	CGCG	2 cut(s)	36, 129
BstV1I	GCAGC	7 cut(s)	36, 301, 1630, 1633, 2028, 2056, 3100
BstX2I	RGATCY	2 cut(s)	814, 1853
BstYI	RGATCY	2 cut(s)	814, 1853
BstZ17I	GTATAC	2 cut(s)	2479, 3390
BstZI	CGGCCG	1 cut(s)	3473
Bsu15I	ATCGAT	1 cut(s)	1248
BsuI	GTATCC	1 cut(s)	818
BsuTUI	ATCGAT	1 cut(s)	1248
BtgI	CCRYGG	5 cut(s)	34, 389, 837, 1100, 1620
BtsCI	GGATG	7 cut(s)	195, 379, 964, 970, 1740, 2156, 2843
BtsI	GCAGTG	2 cut(s)	917, 3016
BtsIMutI	CAGTG	6 cut(s)	917, 1187, 1923, 3016, 3031, 3090
BveI	ACCTGC	1 cut(s)	2083
Cac8I	GCNNGC	6 cut(s)	113, 386, 1041, 1149, 2227, 3051
CaiI	CAGNNNCTG	2 cut(s)	1040, 2243
CciI	TCATGA	1 cut(s)	1678
CciNI	GCGGCCGC	1 cut(s)	3473
CfoI	GCGC	2 cut(s)	305, 576
Cfr42I	CCGCGG	1 cut(s)	37
ClaI	ATCGAT	1 cut(s)	1248
CseI	GACGC	5 cut(s)	199, 317, 935, 3010, 3248
CspCI	CAANNNNNGTGG	2 cut(s)	2325, 2360
DinI	GGCGCC	1 cut(s)	304
DraI	TTTAAA	3 cut(s)	1317, 2398, 2679
DraIII	CACNNNGTG	1 cut(s)	1607
DrdI	GACNNNNNNGTC	1 cut(s)	545
DseDI	GACNNNNNNGTC	1 cut(s)	545
EaeI	YGGCCR	1 cut(s)	3473
EagI	CGGCCG	1 cut(s)	3473
Eam1104I	CTCTTC	4 cut(s)	182, 498, 585, 1947
EarI	CTCTTC	4 cut(s)	182, 498, 585, 1947
EclXI	CGGCCG	1 cut(s)	3473
Eco130I	CCWWGG	6 cut(s)	342, 837, 1620, 2109, 2489, 3098
Eco147I	AGGCCT	1 cut(s)	1510
Eco24I	GRGCYC	2 cut(s)	2462, 2513
Eco31I	GGTCTC	1 cut(s)	1115
Eco47I	GGWCC	3 cut(s)	888, 1714, 3319
Eco52I	CGGCCG	1 cut(s)	3473
Eco57I	CTGAAG	3 cut(s)	774, 923, 1278
Eco88I	CYCGRG	1 cut(s)	1538
EcoNI	CCTNNNNNAGG	1 cut(s)	1367
EcoO109I	RGGNCCY	4 cut(s)	888, 2458, 2459, 3319
EcoRI	GAATTC	1 cut(s)	365
EcoRII	CCWGG	6 cut(s)	524, 1128, 1399, 1505, 1649, 1716
EcoT14I	CCWWGG	6 cut(s)	342, 837, 1620, 2109, 2489, 3098
EcoT22I	ATGCAT	3 cut(s)	805, 2227, 3055
EcoT38I	GRGCYC	2 cut(s)	2462, 2513
EgeI	GGCGCC	1 cut(s)	304
EheI	GGCGCC	1 cut(s)	304
ErhI	CCWWGG	6 cut(s)	342, 837, 1620, 2109, 2489, 3098
Esp3I	CGTCTC	1 cut(s)	286
FalI	AAGNNNNNCTT	2 cut(s)	1776, 1808
FaqI	GGGAC	6 cut(s)	197, 437, 446, 635, 1741, 3251
FauI	CCCGC	9 cut(s)	38, 116, 263, 293, 310, 377, 399, 1140, 2113
FauNDI	CATATG	2 cut(s)	990, 2221
FbaI	TGATCA	1 cut(s)	631
FblI	GTMKAC	4 cut(s)	2085, 2478, 2535, 3389
FokI	GGATG	7 cut(s)	182, 386, 971, 977, 1747, 2163, 2850
FriOI	GRGCYC	2 cut(s)	2462, 2513
FspBI	CTAG	5 cut(s)	1908, 2196, 2370, 3204, 3276
GlaI	GCGC	2 cut(s)	304, 575
GsaI	CCCAGC	2 cut(s)	700, 958
HaeII	RGCGCY	1 cut(s)	306
HapII	CCGG	4 cut(s)	483, 2698, 2803, 2975
HgaI	GACGC	5 cut(s)	199, 317, 935, 3010, 3248
HhaI	GCGC	2 cut(s)	305, 576
Hin1I	GRCGYC	4 cut(s)	210, 303, 309, 946
Hin6I	GCGC	2 cut(s)	303, 574
HinP1I	GCGC	2 cut(s)	303, 574
HincII	GTYRAC	2 cut(s)	331, 1975
HindII	GTYRAC	2 cut(s)	331, 1975
HpaI	GTTAAC	1 cut(s)	1975
HpaII	CCGG	4 cut(s)	483, 2698, 2803, 2975
Hpy166II	GTNNAC	8 cut(s)	331, 379, 1975, 2086, 2479, 2536, 3273, 3390
Hpy8I	GTNNAC	8 cut(s)	331, 379, 1975, 2086, 2479, 2536, 3273, 3390
Hpy99I	CGWCG	4 cut(s)	311, 503, 858, 2865
HpyCH4IV	ACGT	1 cut(s)	3426
HpySE526I	ACGT	1 cut(s)	3426
Hsp92I	GRCGYC	4 cut(s)	210, 303, 309, 946
HspAI	GCGC	2 cut(s)	303, 574
KasI	GGCGCC	1 cut(s)	302
Ksp22I	TGATCA	1 cut(s)	631
KspAI	GTTAAC	1 cut(s)	1975
KspI	CCGCGG	1 cut(s)	37
LmnI	GCTCC	5 cut(s)	346, 1144, 1939, 2384, 3127
Lsp1109I	GCAGC	7 cut(s)	36, 301, 1630, 1633, 2028, 2056, 3100
LweI	GCATC	7 cut(s)	984, 1254, 1684, 1725, 2086, 2230, 2401
MaeI	CTAG	5 cut(s)	1908, 2196, 2370, 3204, 3276
MaeII	ACGT	1 cut(s)	3426
MaeIII	GTNAC	4 cut(s)	1448, 1687, 1987, 3343
MbiI	CCGCTC	2 cut(s)	1147, 3122
MflI	RGATCY	2 cut(s)	814, 1853
MhlI	GDGCHC	7 cut(s)	465, 1153, 1603, 2462, 2513, 3031, 3275
Mly113I	GGCGCC	1 cut(s)	303
MlyI	GAGTC	6 cut(s)	428, 617, 653, 1723, 1994, 2957
MmeI	TCCRAC	6 cut(s)	102, 667, 2823, 3006, 3233, 3444
Mph1103I	ATGCAT	3 cut(s)	805, 2227, 3055
MroXI	GAANNNNTTC	1 cut(s)	1137
MslI	CAYNNNNRTG	9 cut(s)	729, 1026, 1562, 1680, 1683, 2151, 2224, 2942, 3377
MspA1I	CMGCKG	4 cut(s)	36, 314, 676, 2785
MspCI	CTTAAG	1 cut(s)	3323
MspI	CCGG	4 cut(s)	483, 2698, 2803, 2975
MspR9I	CCNGG	9 cut(s)	483, 526, 1130, 1401, 1507, 1651, 1718, 2803, 2975
MssI	GTTTAAAC	1 cut(s)	1317
Mva1269I	GAATGC	4 cut(s)	1077, 2759, 3053, 3340
MvaI	CCWGG	6 cut(s)	526, 1130, 1401, 1507, 1651, 1718
MvnI	CGCG	2 cut(s)	36, 129
NarI	GGCGCC	1 cut(s)	303
NciI	CCSGG	3 cut(s)	483, 2803, 2975
NcoI	CCATGG	2 cut(s)	837, 1620
NdeI	CATATG	2 cut(s)	990, 2221
NlaIV	GGNNCC	9 cut(s)	304, 322, 462, 890, 2270, 2332, 2386, 2460, 3162
NmeAIII	GCCGAG	1 cut(s)	550
NmuCI	GTSAC	2 cut(s)	1448, 1687
NotI	GCGGCCGC	1 cut(s)	3473
NsiI	ATGCAT	3 cut(s)	805, 2227, 3055
NspI	RCATGY	4 cut(s)	2016, 2229, 3053, 3313
NspV	TTCGAA	1 cut(s)	867
PaeI	GCATGC	2 cut(s)	2229, 3053
PaeR7I	CTCGAG	1 cut(s)	1538
PagI	TCATGA	1 cut(s)	1678
PceI	AGGCCT	1 cut(s)	1510
PciI	ACATGT	2 cut(s)	2012, 3309
PctI	GAATGC	4 cut(s)	1077, 2759, 3053, 3340
PdmI	GAANNNNTTC	1 cut(s)	1137
PfeI	GAWTC	5 cut(s)	296, 566, 2181, 3200, 3298
PflFI	GACNNNGTC	1 cut(s)	2886
PfoI	TCCNGGA	1 cut(s)	1716
PleI	GAGTC	6 cut(s)	428, 617, 652, 1723, 1994, 2956
PluTI	GGCGCC	1 cut(s)	306
PmeI	GTTTAAAC	1 cut(s)	1317
PpsI	GAGTC	6 cut(s)	428, 617, 652, 1723, 1994, 2956
PpuMI	RGGWCCY	2 cut(s)	888, 3319
PscI	ACATGT	2 cut(s)	2012, 3309
Psp5II	RGGWCCY	2 cut(s)	888, 3319
Psp6I	CCWGG	6 cut(s)	524, 1128, 1399, 1505, 1649, 1716
PspFI	CCCAGC	2 cut(s)	696, 954
PspGI	CCWGG	6 cut(s)	524, 1128, 1399, 1505, 1649, 1716
PspN4I	GGNNCC	9 cut(s)	304, 322, 462, 890, 2270, 2332, 2386, 2460, 3162
PspOMI	GGGCCC	1 cut(s)	2458
PspPPI	RGGWCCY	2 cut(s)	888, 3319
PspXI	VCTCGAGB	1 cut(s)	1538
PstI	CTGCAG	1 cut(s)	2046
PstNI	CAGNNNCTG	2 cut(s)	1040, 2243
PsuI	RGATCY	2 cut(s)	814, 1853
PsyI	GACNNNGTC	1 cut(s)	2886
PvuII	CAGCTG	1 cut(s)	676
RseI	CAYNNNNRTG	9 cut(s)	729, 1026, 1562, 1680, 1683, 2151, 2224, 2942, 3377
SacII	CCGCGG	1 cut(s)	37
ScaI	AGTACT	3 cut(s)	509, 1536, 1788
SchI	GAGTC	6 cut(s)	428, 617, 653, 1723, 1994, 2957
ScrFI	CCNGG	9 cut(s)	483, 526, 1130, 1401, 1507, 1651, 1718, 2803, 2975
SduI	GDGCHC	7 cut(s)	465, 1153, 1603, 2462, 2513, 3031, 3275
SfaNI	GCATC	7 cut(s)	984, 1254, 1684, 1725, 2086, 2230, 2401
SfcI	CTRYAG	1 cut(s)	2042
SfoI	GGCGCC	1 cut(s)	304
Sfr274I	CTCGAG	1 cut(s)	1538
Sfr303I	CCGCGG	1 cut(s)	37
SfuI	TTCGAA	1 cut(s)	867
SgrBI	CCGCGG	1 cut(s)	37
SinI	GGWCC	3 cut(s)	888, 1714, 3319
SlaI	CTCGAG	1 cut(s)	1538
SmiI	ATTTAAAT	1 cut(s)	2679
SmiMI	CAYNNNNRTG	9 cut(s)	729, 1026, 1562, 1680, 1683, 2151, 2224, 2942, 3377
SmlI	CTYRAG	3 cut(s)	1538, 3323, 3414
SmoI	CTYRAG	3 cut(s)	1538, 3323, 3414
SphI	GCATGC	2 cut(s)	2229, 3053
SseBI	AGGCCT	1 cut(s)	1510
SspDI	GGCGCC	1 cut(s)	302
SspI	AATATT	2 cut(s)	2064, 2664
SspMI	CTAG	5 cut(s)	1908, 2196, 2370, 3204, 3276
StuI	AGGCCT	1 cut(s)	1510
StyD4I	CCNGG	9 cut(s)	481, 524, 1128, 1399, 1505, 1649, 1716, 2801, 2973
StyI	CCWWGG	6 cut(s)	342, 837, 1620, 2109, 2489, 3098
SwaI	ATTTAAAT	1 cut(s)	2679
TaiI	ACGT	1 cut(s)	3429
TatI	WGTACW	6 cut(s)	507, 1191, 1534, 1786, 2364, 2554
TauI	GCSGC	6 cut(s)	36, 39, 314, 3122, 3475, 3478
TfiI	GAWTC	5 cut(s)	296, 566, 2181, 3200, 3298
TscAI	CASTG	6 cut(s)	924, 1194, 1923, 3023, 3031, 3090
TseFI	GTSAC	2 cut(s)	1448, 1687
TseI	GCWGC	7 cut(s)	24, 314, 1643, 1646, 2041, 2044, 3088
Tsp45I	GTSAC	2 cut(s)	1448, 1687
TspGWI	ACGGA	4 cut(s)	807, 842, 1089, 3033
TspRI	CASTG	6 cut(s)	924, 1194, 1923, 3023, 3031, 3090
Tth111I	GACNNNGTC	1 cut(s)	2886
Vha464I	CTTAAG	1 cut(s)	3323
VneI	GTGCAC	1 cut(s)	3271
VpaK11BI	GGWCC	3 cut(s)	888, 1714, 3319
XagI	CCTNNNNNAGG	1 cut(s)	1367
XapI	RAATTY	4 cut(s)	365, 655, 928, 2561
XceI	RCATGY	4 cut(s)	2016, 2229, 3053, 3313
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1657, 1679, 2496
XhoI	CTCGAG	1 cut(s)	1538
XmiI	GTMKAC	4 cut(s)	2085, 2478, 2535, 3389
XmnI	GAANNNNTTC	1 cut(s)	1137
XspI	CTAG	5 cut(s)	1908, 2196, 2370, 3204, 3276
ZrmI	AGTACT	3 cut(s)	509, 1536, 1788
Zsp2I	ATGCAT	3 cut(s)	805, 2227, 3055

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.