Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0010099

Physical Location & Seq

Genomic Coordinates Forward (+)

3094743 .. 3099304

Gene Structure 4562 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1042200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3498 bp

ATGGCTTTCTCGTCGTACATTGGCAGCACCCGCCGCGGCGGCTGGGCCAACTCCCTCCTCCCTTCCTCCTCCGCTTCCAACCCCAAATCCAAGCTCACCAGAAAGCCCCGCAGGCGATTACCCCTCCGCGACTTCATCTTCGCAAACTTCTTCGTTATCGGCCTCTCGATCTCCCTCTTCTTCTTCCTCATCGTCTTCCTCCGCTACGGCGTCCCCACACCCCTCTCCTCCCACTTCAAATCCAAATCCCCCGCCCGCTTCTCCAAACCCCGGAAGCCCGTTTCCCGCAAGAACGTCTCGGCCGCCGACGCCGATGCGGGGGCCACCGTCGACATCACCACCAAGGACCTGTACGACAAGATTGACTTCTCGGATGTGGACGGCGGGCCGTGGAAGCAGGGGTGGCGGGTGAGCTACAAAGGGGACGAGTGGGACTCTGAGAAATTGAAGGTGATCGTGGTGCCCCATTCGCATAACGATCCCGGCTGGAAGCTCACGGTGGAAGAGTACTACGATATGCAATCCAGACATATTCTCGACACCATCGTCGATACGCTTTCGAAGTGCATTGAGTTCGATTCTCGGCGCAAGTTTATATGGGAAGAGATGTCTTATCTGGAAAGATGGTGGAGAGATGCCTCTGATCACAAAAGGGAGTCATTCACCAATTTGGTGAAAAATGGTCAGCTCGAAATTGTTGGAGGTGGCTGGGTAATGAACGATGAGGCTAATTCACATTATTATGCCATAATTGAGCAGATGACAGAAGGAAATATGTGGTTGAACGAAACTGTTGGGGTTATTCCTAAAAATGCTTGGGCAATAGATCCATTTGGATACTCACCTACCATGGCATATCTTCTCCGTCGCATGGGTTTTGAAAATATGCTTATTCAGAGGACCCATTATGAGCTGAAGAAGGAACTTGCACTGCATAAAAATTTGGAGTATGTATGGCGTCAGAGCTGGGATGTGGATGAAACAACCGATATTTTTGTCCACATGATGCCCTTTTATTCTTATGATATTCCACATACTTGTGGTCCAGAGCCTGCTATTTGTTGTCAGTTTGACTTTGCTCGAATGCATGGCTTTGATTATGAACTCTGTCCGTGGAGAGACGATCCAGTAGAGACCAACCAGGGAAATGTTCAGGAGCGGGCACTTATTCTACTAGATCAATATAAGAAGAAATCAACACTGTACAGGACAAATACACTTCTTATTCCTCTTGGAGATGATTTCCGCTACAAAAGCATCGATGAAGCTGAAGCTCAGTTTAGGAACTACCAAATGCTGTTTGATTATATCAATTCTAATCCCAGTTTGAACACAGAGGCCAAGTTTGGAACTCTGGAAGATTACTTTTGGACCCTTCGTGAGGAGGCTGAAAGAATAAATCATTCACTTCCTGGTGAGATTGGTTCCTTTCAGGTTGGGGGTTTTCCTTCTTTGTCAGGTGACTTCTTCACTTATGCTGATAGGCAACAGGACTATTGGAGTGGCTATTATGTTTCCAGGCCTTTCTTCAAGGCTGTTGATCGAGTTCTCGAGCAGACAATTCGTACCACAGATATGATGATTGCTTTCCTGCTTGGGTACTGCCAGAAACCACAATGTGAAAAGTTGCCCATGGGGTTCTCCTACAAGTTGGCTGCAGCTAGGAGGAACTTAGCTCTTTTCCAGCATCACGATGGAGTGACTGGTACTGCTAAAGATCATGTGGTCCTGGACTATGGGACTCGGATGCACACATCTTTGCAGGACTTGCAGATTTTCATGTCTAAAGCTATTGAAGTACTGCTCGGAATGCGCCATGAGAAAAATGACAATAACCCGTCTCAGTTTGAGCCAGAACAGGTGAGATCTAAATATGATGTTCAGCCTGTTCATAGAGCAATCATGGCCCGTGAAGGAACTAGACAGTCGGTGGTGTTTTTTAATCCTTTGGAGCAGACAAGAGAAGAGGTTGTGATGGTTATTGTTAACCGTCCAGATGTTACTGTTCTAGACTCAAACTGGACATGTGTTCAAAGCCAAATATCCCCTGAGCTTCAGCATGATAAAAGCAAAACTTTTACTGGAAGGCACCGTGTCTACTGGCAGGCTTCTGTTCCTGCATTGGGGTTGCAAACATATTACATTGCTAATGGGCTTGTTGGATGTGAAAAGGCCAAACCAGCAAAACTAAAATTTTTCTCAAAGTCTGGTTCTTTATCCTGTCCCACTCCATATGCTTGCTCAAAACCAAAAGCTGATGTGGCTGAAATCCAGAACAGGAACCAAATACTCACCTTTGATGTGAAGCATGGCTTGCTGCAGAAAATAAGCTATAAGAATGGTTCGCAAAATGTTGCGGGTGAGGAAATAGCTATGTACTCTAGCTCGGGAAGTGGAGCCTACCTATTTAAACCGAAAGGTGATGCCCAGCCTATCATTGAAGAAGGTGGGCAGTTGGTGATCTCTGAGGGCCCTTTGGTGCAGGAAGTTTACTCTTATCCAAGGACTGCATGGGAGAAGAGCCCCATTTCTCATAGCACCCGTATCTACAATGGAGAGAATACTGTACAGGAATTTCTCATTGAGAAGGAGTATCATGTTGAGCTTCTCGGCCAGGAGTTTGATGACAAGGAGTTGATAGTTAGGTATAAAACAGATGTTGATAACAAAAGAATATTCTTTTCTGATTTAAATGGCTTTCAGATGAGCCGGAGAGAAACCTATGATAAAATCCCAGTTCAGGGCAATTATTACCCTATGCCCTCTCTTGCATTCATGCAGGGCTCCAATGGTCAGCGGTTTTCAGTCCATTCCCGGCAGGCGTTGGGTGTCGCAAGTCTTAAAAATGGATGGTTGGAGATTATGCTTGACCGTCGTTTGGTAAGAGATGATGGACGAGGTCTTGGACAAGGAGTGATGGACAACCGTGCTATGAATGTAATCTTCCACATCGTTGTGGAGTCTAACATTTCAGCCACATCAAACCCAGTTTCCAACCCATTGCCCTTGAACCCCTCTCTTCTTTCTCACCGTGTCAGTACTCACCTGAACTATCCGTTGCATGCATTCATTAACAAGAAGCCAGAGGAGTTAACAGTGCAGCCACCCCCAAGATCTTTCTCCCCCTTAGCCGCTTCCTTACCATGTGATCTGCATATTGTAAGTTTTAAGGTTCCCCAACCTCTAAAATACACTCAGCAACCTCTCGGAGATTCTAGGTTTGCGCTAATTTTACAGAGACAGAACTGGGACTCTTCATATTGTCGGAAGGGCAGATCAGGGTGCACTAGATTTGCTGATGAGACTGTGAATCTCTTTTACATGTTCAAAGACCTTGCGGTATTTAATGCAAGAGCTACTTCCTTAAATCTTTTGCATGAGGACATGGATATGCTTGGGTATACTGATCAATTTGGAGATGTTGCTCAAGACGGACACGTTCTTATGTCTCCAATGGAAATACAGGCTTACAAGTTGGAATTGCGGCCGCACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0004572 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006486 GO:0006487 GO:0006491 GO:0006517 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0015923 GO:0015924 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0034645 GO:0036211 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0070085 GO:0071704 GO:0097708 GO:0098588 GO:0098791 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko00513 ko01100 map00510 map00513 map01100

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1166

Amino Acids

133.65

Weight (kDa)

6.81

Isoelectric Point (pI)

45.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	150 - 492	3.8e-80	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	499 - 601	2.5e-19	Alpha mannosidase middle domain
Glyco_hydro_38C	PF07748	757 - 966	4.4e-31	Glycosyl hydrolases family 38 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010099)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14950
fragaria_vesca	FvH4_6g49910 FvH4_6g49910
malus_domestica	MD09G1040900.v1.1 MD17G1042200.v1.1
prunus_persica	Prupe.3G276800_v2.0.a1
pyrus_communis	pycom111g03190 pycom17g03770
rosa_chinensis	RchiOBHm_Chr2g0170081
rosa_laevigata	RLG00000021920
rosa_multiflora	Rmu_co8321257.1_g000001 Rmu_sc0013078.1_g000007 Rmu_sc0013453.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081600
rosa_rugosa	Rorug02G0545500 Rorug02G0545600.1
rosa_samantha	Rh2AG617700 Rh2BG629200 Rh2CG598600 Rh2DG641000
rosa_wichuraiana	Rw2G051140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	545
AccB1I	GGYRCC	2 cut(s)	460, 2088
AccBSI	CCGCTC	1 cut(s)	1159
AccI	GTMKAC	3 cut(s)	330, 2097, 3401
AccII	CGCG	2 cut(s)	36, 129
AclWI	GGATC	3 cut(s)	473, 821, 1118
AcoI	YGGCCR	3 cut(s)	300, 2611, 3485
AcsI	RAATTY	3 cut(s)	940, 2192, 2573
AcuI	CTGAAG	3 cut(s)	935, 1290, 2039
AcyI	GRCGYC	3 cut(s)	210, 309, 958
AdeI	CACNNNGTG	1 cut(s)	1619
AfiI	CCNNNNNNNGG	8 cut(s)	270, 318, 871, 1381, 2123, 2740, 2741, 2878
AflIII	ACRYGT	3 cut(s)	2024, 3321, 3436
AjnI	CCWGG	5 cut(s)	1140, 1411, 1517, 1728, 2613
Alw21I	GWGCWC	1 cut(s)	3287
Alw26I	GTCTC	7 cut(s)	301, 1113, 1127, 1845, 3232, 3296, 3453
Alw44I	GTGCAC	1 cut(s)	3283
AlwI	GGATC	3 cut(s)	473, 821, 1118
AlwNI	CAGNNNCTG	1 cut(s)	1052
Ama87I	CYCGRG	2 cut(s)	1550, 2386
ApaI	GGGCCC	1 cut(s)	2474
ApaLI	GTGCAC	1 cut(s)	3283
ApeKI	GCWGC	5 cut(s)	24, 1655, 1658, 2317, 3100
ApoI	RAATTY	3 cut(s)	940, 2192, 2573
ArsI	GACNNNNNNTTYG	2 cut(s)	122, 154
AspLEI	GCGC	3 cut(s)	588, 1815, 3226
AsuC2I	CCSGG	3 cut(s)	271, 483, 2815
AsuII	TTCGAA	1 cut(s)	560
AvaI	CYCGRG	2 cut(s)	1550, 2386
AvaII	GGWCC	5 cut(s)	346, 900, 1043, 1371, 1726
BaeGI	GKGCMC	4 cut(s)	465, 1165, 2474, 3287
BanI	GGYRCC	2 cut(s)	460, 2088
BanII	GRGCYC	3 cut(s)	2474, 2525, 2786
BbsI	GAAGAC	1 cut(s)	187
Bbv12I	GWGCWC	1 cut(s)	3287
BbvI	GCAGC	5 cut(s)	36, 1642, 1670, 2304, 3112
BccI	CCATC	7 cut(s)	551, 618, 1688, 1969, 2844, 2885, 2911
BceAI	ACGGC	3 cut(s)	223, 373, 397
BcgI	CGANNNNNNTGC	6 cut(s)	296, 330, 556, 590, 1544, 1578
BciT130I	CCWGG	5 cut(s)	1142, 1413, 1519, 1730, 2615
BciVI	GTATCC	1 cut(s)	830
BclI	TGATCA	2 cut(s)	643, 3406
BcnI	CCSGG	3 cut(s)	271, 483, 2815
BcoDI	GTCTC	7 cut(s)	301, 1113, 1127, 1845, 3232, 3296, 3453
BfaI	CTAG	7 cut(s)	1175, 1662, 1920, 2009, 2382, 3216, 3288
BfmI	CTRYAG	2 cut(s)	1656, 2318
BfuI	GTATCC	1 cut(s)	830
BglI	GCCNNNNNGGC	2 cut(s)	39, 112
BglII	AGATCT	2 cut(s)	1865, 3113
BmcAI	AGTACT	3 cut(s)	509, 1800, 3040
Bme1390I	CCNGG	8 cut(s)	271, 483, 1142, 1413, 1519, 1730, 2615, 2815
Bme18I	GGWCC	5 cut(s)	346, 900, 1043, 1371, 1726
BmeT110I	CYCGRG	2 cut(s)	1550, 2386
BmrFI	CCNGG	8 cut(s)	271, 483, 1142, 1413, 1519, 1730, 2615, 2815
BmrI	ACTGGG	4 cut(s)	1317, 2729, 2981, 3256
BmsI	GCATC	7 cut(s)	304, 625, 996, 1266, 1696, 1737, 2413
BmuI	ACTGGG	4 cut(s)	1317, 2729, 2981, 3256
BpiI	GAAGAC	1 cut(s)	187
BplI	GAGNNNNNCTC	2 cut(s)	419, 451
Bpu10I	CCTNAGC	2 cut(s)	2049, 3127
Bpu14I	TTCGAA	1 cut(s)	560
BpuEI	CTTGAG	1 cut(s)	3411
BpuMI	CCSGG	3 cut(s)	271, 483, 2815
Bsa29I	ATCGAT	1 cut(s)	1260
BsaHI	GRCGYC	3 cut(s)	210, 309, 958
BsaI	GGTCTC	1 cut(s)	1127
BsaJI	CCNNGG	9 cut(s)	34, 269, 342, 389, 849, 1112, 1141, 1632, 2501
Bsc4I	CCNNNNNNNGG	8 cut(s)	270, 318, 871, 1381, 2123, 2740, 2741, 2878
Bse1I	ACTGG	9 cut(s)	1127, 1323, 1708, 2024, 2086, 2105, 2735, 2987, 3251
Bse3DI	GCAATG	2 cut(s)	2142, 2999
BseBI	CCWGG	5 cut(s)	1142, 1413, 1519, 1730, 2615
BseCI	ATCGAT	1 cut(s)	1260
BseDI	CCNNGG	9 cut(s)	34, 269, 342, 389, 849, 1112, 1141, 1632, 2501
BseGI	GGATG	6 cut(s)	379, 976, 982, 1752, 2168, 2855
BseLI	CCNNNNNNNGG	8 cut(s)	270, 318, 871, 1381, 2123, 2740, 2741, 2878
BseMI	GCAATG	2 cut(s)	2142, 2999
BseMII	CTCAG	6 cut(s)	429, 1289, 1856, 2040, 2457, 3209
BseNI	ACTGG	9 cut(s)	1127, 1323, 1708, 2024, 2086, 2105, 2735, 2987, 3251
BseRI	GAGGAG	5 cut(s)	47, 58, 217, 1397, 3101
BseSI	GKGCMC	4 cut(s)	465, 1165, 2474, 3287
BseX3I	CGGCCG	2 cut(s)	300, 3485
BseXI	GCAGC	5 cut(s)	36, 1642, 1670, 2304, 3112
BseYI	CCCAGC	4 cut(s)	42, 708, 966, 2427
BsgI	GTGCAG	2 cut(s)	2501, 3119
Bsh1236I	CGCG	2 cut(s)	36, 129
Bsh1285I	CGRYCG	2 cut(s)	303, 3488
BshNI	GGYRCC	2 cut(s)	460, 2088
BshVI	ATCGAT	1 cut(s)	1260
BsiEI	CGRYCG	2 cut(s)	303, 3488
BsiHKAI	GWGCWC	1 cut(s)	3287
BsiHKCI	CYCGRG	2 cut(s)	1550, 2386
BsiSI	CCGG	4 cut(s)	271, 483, 2710, 2815
BslFI	GGGAC	6 cut(s)	197, 437, 446, 1753, 2208, 3263
BslI	CCNNNNNNNGG	8 cut(s)	270, 318, 871, 1381, 2123, 2740, 2741, 2878
BsmAI	GTCTC	7 cut(s)	301, 1113, 1127, 1845, 3232, 3296, 3453
BsmBI	CGTCTC	3 cut(s)	301, 1113, 1845
BsmFI	GGGAC	6 cut(s)	197, 437, 446, 1753, 2208, 3263
BsmI	GAATGC	4 cut(s)	1089, 1815, 2771, 3065
Bso31I	GGTCTC	1 cut(s)	1127
BsoBI	CYCGRG	2 cut(s)	1550, 2386
Bsp119I	TTCGAA	1 cut(s)	560
Bsp120I	GGGCCC	1 cut(s)	2470
Bsp1286I	GDGCHC	6 cut(s)	465, 1165, 2474, 2525, 2786, 3287
Bsp1407I	TGTACA	2 cut(s)	1203, 2566
Bsp19I	CCATGG	2 cut(s)	849, 1632
BspCNI	CTCAG	6 cut(s)	430, 1288, 1855, 2041, 2458, 3208
BspDI	ATCGAT	1 cut(s)	1260
BspFNI	CGCG	2 cut(s)	36, 129
BspMAI	CTGCAG	2 cut(s)	1660, 2322
BspPI	GGATC	3 cut(s)	473, 821, 1118
BspQI	GCTCTTC	1 cut(s)	2513
BspT104I	TTCGAA	1 cut(s)	560
BspT107I	GGYRCC	2 cut(s)	460, 2088
BspTNI	GGTCTC	1 cut(s)	1127
BsrBI	CCGCTC	1 cut(s)	1159
BsrDI	GCAATG	2 cut(s)	2142, 2999
BsrGI	TGTACA	2 cut(s)	1203, 2566
BsrI	ACTGG	9 cut(s)	1127, 1323, 1708, 2024, 2086, 2105, 2735, 2987, 3251
BssECI	CCNNGG	9 cut(s)	34, 269, 342, 389, 849, 1112, 1141, 1632, 2501
BssNAI	GTATAC	1 cut(s)	3402
BssNI	GRCGYC	3 cut(s)	210, 309, 958
BssT1I	CCWWGG	4 cut(s)	342, 849, 1632, 2501
Bst1107I	GTATAC	1 cut(s)	3402
Bst2UI	CCWGG	5 cut(s)	1142, 1413, 1519, 1730, 2615
Bst6I	CTCTTC	7 cut(s)	182, 498, 597, 1959, 2513, 3024, 3259
BstACI	GRCGYC	3 cut(s)	210, 309, 958
BstAPI	GCANNNNNTGC	2 cut(s)	1768, 2314
BstAUI	TGTACA	2 cut(s)	1203, 2566
BstBI	TTCGAA	1 cut(s)	560
BstDEI	CTNAG	8 cut(s)	438, 1275, 1672, 1842, 2049, 2466, 3127, 3195
BstDSI	CCRYGG	5 cut(s)	34, 389, 849, 1112, 1632
BstENI	CCTNNNNNAGG	1 cut(s)	1379
BstF5I	GGATG	6 cut(s)	379, 976, 982, 1752, 2168, 2855
BstFNI	CGCG	2 cut(s)	36, 129
BstHHI	GCGC	3 cut(s)	588, 1815, 3226
BstMAI	GTCTC	7 cut(s)	301, 1113, 1127, 1845, 3232, 3296, 3453
BstMCI	CGRYCG	2 cut(s)	303, 3488
BstNI	CCWGG	5 cut(s)	1142, 1413, 1519, 1730, 2615
BstNSI	RCATGY	3 cut(s)	2028, 3065, 3325
BstSCI	CCNGG	8 cut(s)	269, 481, 1140, 1411, 1517, 1728, 2613, 2813
BstSFI	CTRYAG	2 cut(s)	1656, 2318
BstSLI	GKGCMC	4 cut(s)	465, 1165, 2474, 3287
BstUI	CGCG	2 cut(s)	36, 129
BstV1I	GCAGC	5 cut(s)	36, 1642, 1670, 2304, 3112
BstV2I	GAAGAC	1 cut(s)	187
BstX2I	RGATCY	3 cut(s)	826, 1865, 3113
BstYI	RGATCY	3 cut(s)	826, 1865, 3113
BstZ17I	GTATAC	1 cut(s)	3402
BstZI	CGGCCG	2 cut(s)	300, 3485
Bsu15I	ATCGAT	1 cut(s)	1260
BsuI	GTATCC	1 cut(s)	830
BsuTUI	ATCGAT	1 cut(s)	1260
BtgI	CCRYGG	5 cut(s)	34, 389, 849, 1112, 1632
BtsCI	GGATG	6 cut(s)	379, 976, 982, 1752, 2168, 2855
BtsI	GCAGTG	1 cut(s)	929
BtsIMutI	CAGTG	3 cut(s)	929, 1199, 3102
CaiI	CAGNNNCTG	1 cut(s)	1052
CciNI	GCGGCCGC	1 cut(s)	3485
CfoI	GCGC	3 cut(s)	588, 1815, 3226
Cfr42I	CCGCGG	1 cut(s)	37
ClaI	ATCGAT	1 cut(s)	1260
CseI	GACGC	3 cut(s)	199, 317, 947
DdeI	CTNAG	8 cut(s)	438, 1275, 1672, 1842, 2049, 2466, 3127, 3195
DraI	TTTAAA	2 cut(s)	2410, 2691
DraIII	CACNNNGTG	1 cut(s)	1619
DrdI	GACNNNNNNGTC	1 cut(s)	545
DseDI	GACNNNNNNGTC	1 cut(s)	545
EaeI	YGGCCR	3 cut(s)	300, 2611, 3485
EagI	CGGCCG	2 cut(s)	300, 3485
Eam1104I	CTCTTC	7 cut(s)	182, 498, 597, 1959, 2513, 3024, 3259
EarI	CTCTTC	7 cut(s)	182, 498, 597, 1959, 2513, 3024, 3259
EclXI	CGGCCG	2 cut(s)	300, 3485
Eco130I	CCWWGG	4 cut(s)	342, 849, 1632, 2501
Eco147I	AGGCCT	1 cut(s)	1522
Eco24I	GRGCYC	3 cut(s)	2474, 2525, 2786
Eco31I	GGTCTC	1 cut(s)	1127
Eco47I	GGWCC	5 cut(s)	346, 900, 1043, 1371, 1726
Eco52I	CGGCCG	2 cut(s)	300, 3485
Eco57I	CTGAAG	3 cut(s)	935, 1290, 2039
Eco88I	CYCGRG	2 cut(s)	1550, 2386
EcoNI	CCTNNNNNAGG	1 cut(s)	1379
EcoO109I	RGGNCCY	4 cut(s)	346, 900, 2470, 2471
EcoRII	CCWGG	5 cut(s)	1140, 1411, 1517, 1728, 2613
EcoT14I	CCWWGG	4 cut(s)	342, 849, 1632, 2501
EcoT22I	ATGCAT	2 cut(s)	1089, 3067
EcoT38I	GRGCYC	3 cut(s)	2474, 2525, 2786
ErhI	CCWWGG	4 cut(s)	342, 849, 1632, 2501
Esp3I	CGTCTC	3 cut(s)	301, 1113, 1845
FalI	AAGNNNNNCTT	8 cut(s)	350, 382, 2059, 2091, 2297, 2329, 3343, 3375
FaqI	GGGAC	6 cut(s)	197, 437, 446, 1753, 2208, 3263
FauNDI	CATATG	1 cut(s)	2233
FbaI	TGATCA	2 cut(s)	643, 3406
FblI	GTMKAC	3 cut(s)	330, 2097, 3401
FokI	GGATG	6 cut(s)	386, 983, 989, 1759, 2175, 2862
FriOI	GRGCYC	3 cut(s)	2474, 2525, 2786
FspBI	CTAG	7 cut(s)	1175, 1662, 1920, 2009, 2382, 3216, 3288
GlaI	GCGC	3 cut(s)	587, 1814, 3225
GsaI	CCCAGC	4 cut(s)	46, 712, 970, 2431
HapII	CCGG	4 cut(s)	271, 483, 2710, 2815
HgaI	GACGC	3 cut(s)	199, 317, 947
HhaI	GCGC	3 cut(s)	588, 1815, 3226
Hin1I	GRCGYC	3 cut(s)	210, 309, 958
Hin6I	GCGC	3 cut(s)	586, 1813, 3224
HinP1I	GCGC	3 cut(s)	586, 1813, 3224
HincII	GTYRAC	3 cut(s)	331, 1987, 3093
HindII	GTYRAC	3 cut(s)	331, 1987, 3093
HinfI	GANTC	9 cut(s)	434, 578, 656, 1741, 2012, 2960, 3212, 3251, 3310
HpaI	GTTAAC	2 cut(s)	1987, 3093
HpaII	CCGG	4 cut(s)	271, 483, 2710, 2815
Hpy166II	GTNNAC	9 cut(s)	331, 379, 1000, 1987, 2098, 2491, 3093, 3285, 3402
Hpy8I	GTNNAC	9 cut(s)	331, 379, 1000, 1987, 2098, 2491, 3093, 3285, 3402
Hpy99I	CGWCG	6 cut(s)	16, 311, 332, 551, 870, 2877
HpyCH4IV	ACGT	2 cut(s)	294, 3438
HpyF3I	CTNAG	8 cut(s)	438, 1275, 1672, 1842, 2049, 2466, 3127, 3195
HpySE526I	ACGT	2 cut(s)	294, 3438
Hsp92I	GRCGYC	3 cut(s)	210, 309, 958
HspAI	GCGC	3 cut(s)	586, 1813, 3224
Ksp22I	TGATCA	2 cut(s)	643, 3406
KspAI	GTTAAC	2 cut(s)	1987, 3093
KspI	CCGCGG	1 cut(s)	37
LguI	GCTCTTC	1 cut(s)	2513
LmnI	GCTCC	4 cut(s)	1156, 1951, 2396, 2789
Lsp1109I	GCAGC	5 cut(s)	36, 1642, 1670, 2304, 3112
LweI	GCATC	7 cut(s)	304, 625, 996, 1266, 1696, 1737, 2413
MaeI	CTAG	7 cut(s)	1175, 1662, 1920, 2009, 2382, 3216, 3288
MaeII	ACGT	2 cut(s)	294, 3438
MaeIII	GTNAC	3 cut(s)	1460, 1699, 1999
MbiI	CCGCTC	1 cut(s)	1159
MflI	RGATCY	3 cut(s)	826, 1865, 3113
MhlI	GDGCHC	6 cut(s)	465, 1165, 2474, 2525, 2786, 3287
MlyI	GAGTC	6 cut(s)	428, 665, 1735, 2006, 2969, 3245
MmeI	TCCRAC	7 cut(s)	102, 679, 2140, 2835, 3018, 3245, 3456
Mph1103I	ATGCAT	2 cut(s)	1089, 3067
MslI	CAYNNNNRTG	6 cut(s)	741, 1038, 1574, 1692, 2954, 3389
MspA1I	CMGCKG	2 cut(s)	36, 2797
MspI	CCGG	4 cut(s)	271, 483, 2710, 2815
MspR9I	CCNGG	8 cut(s)	271, 483, 1142, 1413, 1519, 1730, 2615, 2815
Mva1269I	GAATGC	4 cut(s)	1089, 1815, 2771, 3065
MvaI	CCWGG	5 cut(s)	1142, 1413, 1519, 1730, 2615
MvnI	CGCG	2 cut(s)	36, 129
NciI	CCSGG	3 cut(s)	271, 483, 2815
NcoI	CCATGG	2 cut(s)	849, 1632
NdeI	CATATG	1 cut(s)	2233
NmeAIII	GCCGAG	3 cut(s)	278, 562, 2589
NmuCI	GTSAC	2 cut(s)	1460, 1699
NotI	GCGGCCGC	1 cut(s)	3485
NsiI	ATGCAT	2 cut(s)	1089, 3067
NspI	RCATGY	3 cut(s)	2028, 3065, 3325
NspV	TTCGAA	1 cut(s)	560
PaeI	GCATGC	1 cut(s)	3065
PaeR7I	CTCGAG	1 cut(s)	1550
PceI	AGGCCT	1 cut(s)	1522
PciI	ACATGT	2 cut(s)	2024, 3321
PciSI	GCTCTTC	1 cut(s)	2513
PcsI	WCGNNNNNNNCGW	1 cut(s)	543
PctI	GAATGC	4 cut(s)	1089, 1815, 2771, 3065
PfeI	GAWTC	3 cut(s)	578, 3212, 3310
PflFI	GACNNNGTC	1 cut(s)	2898
PfoI	TCCNGGA	1 cut(s)	1728
PleI	GAGTC	6 cut(s)	428, 664, 1735, 2006, 2968, 3245
PpsI	GAGTC	6 cut(s)	428, 664, 1735, 2006, 2968, 3245
PpuMI	RGGWCCY	2 cut(s)	346, 900
PscI	ACATGT	2 cut(s)	2024, 3321
Psp5II	RGGWCCY	2 cut(s)	346, 900
Psp6I	CCWGG	5 cut(s)	1140, 1411, 1517, 1728, 2613
PspFI	CCCAGC	4 cut(s)	42, 708, 966, 2427
PspGI	CCWGG	5 cut(s)	1140, 1411, 1517, 1728, 2613
PspOMI	GGGCCC	1 cut(s)	2470
PspPPI	RGGWCCY	2 cut(s)	346, 900
PstI	CTGCAG	2 cut(s)	1660, 2322
PstNI	CAGNNNCTG	1 cut(s)	1052
PsuI	RGATCY	3 cut(s)	826, 1865, 3113
PsyI	GACNNNGTC	1 cut(s)	2898
RseI	CAYNNNNRTG	6 cut(s)	741, 1038, 1574, 1692, 2954, 3389
SacII	CCGCGG	1 cut(s)	37
SalI	GTCGAC	1 cut(s)	329
SapI	GCTCTTC	1 cut(s)	2513
ScaI	AGTACT	3 cut(s)	509, 1800, 3040
SchI	GAGTC	6 cut(s)	428, 665, 1735, 2006, 2969, 3245
ScrFI	CCNGG	8 cut(s)	271, 483, 1142, 1413, 1519, 1730, 2615, 2815
SduI	GDGCHC	6 cut(s)	465, 1165, 2474, 2525, 2786, 3287
SfaNI	GCATC	7 cut(s)	304, 625, 996, 1266, 1696, 1737, 2413
SfcI	CTRYAG	2 cut(s)	1656, 2318
Sfr274I	CTCGAG	1 cut(s)	1550
Sfr303I	CCGCGG	1 cut(s)	37
SfuI	TTCGAA	1 cut(s)	560
SgrBI	CCGCGG	1 cut(s)	37
SinI	GGWCC	5 cut(s)	346, 900, 1043, 1371, 1726
SlaI	CTCGAG	1 cut(s)	1550
SmiI	ATTTAAAT	1 cut(s)	2691
SmiMI	CAYNNNNRTG	6 cut(s)	741, 1038, 1574, 1692, 2954, 3389
SmlI	CTYRAG	2 cut(s)	1550, 3426
SmoI	CTYRAG	2 cut(s)	1550, 3426
SphI	GCATGC	1 cut(s)	3065
SseBI	AGGCCT	1 cut(s)	1522
SspI	AATATT	1 cut(s)	2676
SspMI	CTAG	7 cut(s)	1175, 1662, 1920, 2009, 2382, 3216, 3288
StuI	AGGCCT	1 cut(s)	1522
StyD4I	CCNGG	8 cut(s)	269, 481, 1140, 1411, 1517, 1728, 2613, 2813
StyI	CCWWGG	4 cut(s)	342, 849, 1632, 2501
SwaI	ATTTAAAT	1 cut(s)	2691
TaiI	ACGT	2 cut(s)	297, 3441
TatI	WGTACW	6 cut(s)	507, 1203, 1798, 2376, 2566, 3038
TauI	GCSGC	7 cut(s)	36, 39, 42, 305, 3134, 3487, 3490
TfiI	GAWTC	3 cut(s)	578, 3212, 3310
TscAI	CASTG	3 cut(s)	936, 1206, 3102
TseFI	GTSAC	2 cut(s)	1460, 1699
TseI	GCWGC	5 cut(s)	24, 1655, 1658, 2317, 3100
Tsp45I	GTSAC	2 cut(s)	1460, 1699
TspGWI	ACGGA	4 cut(s)	854, 1101, 3045, 3447
TspRI	CASTG	3 cut(s)	936, 1206, 3102
Tth111I	GACNNNGTC	1 cut(s)	2898
VneI	GTGCAC	1 cut(s)	3283
VpaK11BI	GGWCC	5 cut(s)	346, 900, 1043, 1371, 1726
XagI	CCTNNNNNAGG	1 cut(s)	1379
XapI	RAATTY	3 cut(s)	940, 2192, 2573
XbaI	TCTAGA	1 cut(s)	2008
XceI	RCATGY	3 cut(s)	2028, 3065, 3325
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1691, 2508
XhoI	CTCGAG	1 cut(s)	1550
XmiI	GTMKAC	3 cut(s)	330, 2097, 3401
XspI	CTAG	7 cut(s)	1175, 1662, 1920, 2009, 2382, 3216, 3288
ZrmI	AGTACT	3 cut(s)	509, 1800, 3040
Zsp2I	ATGCAT	2 cut(s)	1089, 3067

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.