FvH4_6g50621

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010660
Physical Location & Seq
Reverse (-)
37647124 .. 37648349
1226 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50621.t1

Sequence Viewer

Length: 414 bp
ATGGCGGATACAAAACCAGAAAAGAGGAAACCTGTTTTTAAAAAGGTTATGCAGCTGCAGCCGGACACCAAGGGGCACACTCTGATTGTAAAAGTGGTAAGTTCGAAGATGGTGTTGCAGAAGGCCCGTCCTGATGGGATCCAAGTTCGTCAGATGAAAATTGCTGAATGCTTGGTTGGAGACGAAACCGGAACCATCGTTTTCACCGCTAGAAATGAACAAGTGGACATGATGAACCCTGGTGCTACCGTGATTTTGAGGAATGCGAAAATTGACATGTTCAAGGGATCTATGAGGCTTGCTGTGGACAAGTGGGGTCGAGTAGAAGTGGCCGAACCGGGTAGCTTCAATGTCAAGGAAGATAACAATATGTCTTCGGTTGAATATGAACTGGTTAGTGTTGTCGATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.32

Weight (kDa)

9.24

Isoelectric Point (pI)

22.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 110 5.5e-46 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 207
AclWI GGATC 3 cut(s) 133, 146, 295
AcoI YGGCCR 1 cut(s) 330
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 3 cut(s) 283, 349, 383
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 2 cut(s) 55, 345
AluI AGCT 2 cut(s) 55, 345
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 3 cut(s) 133, 146, 295
AoxI GGCC 2 cut(s) 123, 330
ApeKI GCWGC 3 cut(s) 52, 55, 58
AspS9I GGNCC 1 cut(s) 124
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 1 cut(s) 196
AsuII TTCGAA 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 78
BamHI GGATCC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 42, 64, 70
BccI CCATC 3 cut(s) 103, 128, 203
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 210
BfmI CTRYAG 1 cut(s) 56
BisI GCNGC 3 cut(s) 53, 56, 59
BlsI GCNGC 3 cut(s) 54, 57, 60
Bme1390I CCNGG 2 cut(s) 240, 339
BmgT120I GGNCC 1 cut(s) 124
BmiI GGNNCC 2 cut(s) 140, 193
BmrFI CCNGG 2 cut(s) 240, 339
BpiI GAAGAC 1 cut(s) 366
Bpu14I TTCGAA 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 69, 238
BsaWI WCCGGW 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 396
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 69, 238
BseNI ACTGG 1 cut(s) 396
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 3 cut(s) 42, 64, 70
BshFI GGCC 2 cut(s) 125, 332
BsiSI CCGG 3 cut(s) 62, 189, 338
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsmI GAATGC 2 cut(s) 173, 268
BsnI GGCC 2 cut(s) 125, 332
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 2 cut(s) 138, 287
BspACI CCGC 2 cut(s) 5, 207
BspANI GGCC 2 cut(s) 125, 332
BspLI GGNNCC 2 cut(s) 140, 193
BspMAI CTGCAG 1 cut(s) 60
BspPI GGATC 3 cut(s) 133, 146, 295
BspT104I TTCGAA 1 cut(s) 104
BsrI ACTGG 1 cut(s) 396
BssECI CCNNGG 2 cut(s) 69, 238
BssMI GATC 2 cut(s) 138, 287
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 250
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 300
BstKTI GATC 2 cut(s) 141, 290
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 2 cut(s) 138, 287
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 2 cut(s) 238, 337
BstSFI CTRYAG 1 cut(s) 56
BstSLI GKGCMC 1 cut(s) 78
BstV1I GCAGC 3 cut(s) 42, 64, 70
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 2 cut(s) 138, 287
BstYI RGATCY 2 cut(s) 138, 287
BsuRI GGCC 2 cut(s) 125, 332
Cac8I GCNNGC 1 cut(s) 300
Cfr13I GGNCC 1 cut(s) 124
CviAII CATG 2 cut(s) 229, 277
CviJI RGCY 6 cut(s) 55, 61, 125, 298, 332, 345
CviKI_1 RGCY 6 cut(s) 55, 61, 125, 298, 332, 345
DpnI GATC 2 cut(s) 140, 289
DpnII GATC 2 cut(s) 138, 287
DraI TTTAAA 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 330
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 2 cut(s) 232, 280
FaiI YATR 6 cut(s) 50, 230, 278, 293, 371, 387
FatI CATG 2 cut(s) 228, 276
Fnu4HI GCNGC 3 cut(s) 53, 56, 59
Fsp4HI GCNGC 3 cut(s) 53, 56, 59
FspBI CTAG 1 cut(s) 210
GluI GCNGC 3 cut(s) 53, 56, 59
HaeIII GGCC 2 cut(s) 125, 332
HapII CCGG 3 cut(s) 62, 189, 338
Hin1II CATG 2 cut(s) 232, 280
HpaII CCGG 3 cut(s) 62, 189, 338
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 2 cut(s) 226, 307
Hpy188I TCNGA 2 cut(s) 84, 153
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 2 cut(s) 226, 307
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 3 cut(s) 52, 58, 118
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
Hsp92II CATG 2 cut(s) 232, 280
Kzo9I GATC 2 cut(s) 138, 287
LpnPI CCDG 9 cut(s) 30, 45, 75, 144, 202, 225, 252, 351, 377
Lsp1109I GCAGC 3 cut(s) 42, 64, 70
MaeI CTAG 1 cut(s) 210
MalI GATC 2 cut(s) 140, 289
MboI GATC 2 cut(s) 138, 287
MboII GAAGA 3 cut(s) 118, 366, 371
MflI RGATCY 2 cut(s) 138, 287
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 2 cut(s) 159, 270
MmeI TCCRAC 1 cut(s) 157
MnlI CCTC 3 cut(s) 18, 252, 288
MseI TTAA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 55
MspI CCGG 3 cut(s) 62, 189, 338
MspR9I CCNGG 2 cut(s) 240, 339
Mva1269I GAATGC 2 cut(s) 173, 268
MvaI CCWGG 1 cut(s) 240
MwoI GCNNNNNNNGC 1 cut(s) 58
NciI CCSGG 1 cut(s) 339
NdeII GATC 2 cut(s) 138, 287
NlaIII CATG 2 cut(s) 232, 280
NlaIV GGNNCC 2 cut(s) 140, 193
NspI RCATGY 1 cut(s) 280
NspV TTCGAA 1 cut(s) 104
PciI ACATGT 1 cut(s) 276
PctI GAATGC 2 cut(s) 173, 268
PkrI GCNGC 3 cut(s) 54, 57, 60
PscI ACATGT 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 140, 193
PspPI GGNCC 1 cut(s) 124
PstI CTGCAG 1 cut(s) 60
PsuI RGATCY 2 cut(s) 138, 287
PvuII CAGCTG 1 cut(s) 55
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 3 cut(s) 53, 56, 59
Sau3AI GATC 2 cut(s) 138, 287
Sau96I GGNCC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 240, 339
SduI GDGCHC 1 cut(s) 78
SetI ASST 4 cut(s) 34, 48, 57, 347
SfcI CTRYAG 1 cut(s) 56
SfuI TTCGAA 1 cut(s) 104
Sse9I AATT 2 cut(s) 159, 270
SsiI CCGC 2 cut(s) 5, 207
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 2 cut(s) 238, 337
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 250
TaqI TCGA 3 cut(s) 104, 319, 405
TasI AATT 2 cut(s) 159, 270
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TseI GCWGC 3 cut(s) 52, 55, 58
TspDTI ATGAA 4 cut(s) 170, 231, 248, 402
XceI RCATGY 1 cut(s) 280
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.