MD09G1034500.v1.1

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010660
Physical Location & Seq
Forward (+)
2115565 .. 2116991
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1034500.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGTATTGCAGAAGGCCCGTCCTGATGGAATCCGAGTTCGTCAGATGAGGATCGCCGAATGCTTGGTCGGAGATGAAACCGGAACCATCGTTTTCACTGCCAGAAATGACCAAGTTGATTTGATGACAACTGGTGCCACGGTGATTCTGCGCAACGCGAAAATAGACATGTTCAAGGGTTCTATGAGGCTTGCTGTGGACAAATGGGGTCGAGTTGAAGTAACCGAACCCGCCAGCTTCTCTGTTAAGGAAGATAACAACTTGTCACTGGTGGAATATGAGCTGATTAATGTTAATGAACTGGCTAATCCTGGTGCCAGTCAAGTGGTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.27

Weight (kDa)

5.11

Isoelectric Point (pI)

17.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 5 - 74 1.1e-35 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 152
AccB1I GGYRCC 2 cut(s) 134, 314
AccII CGCG 1 cut(s) 158
AciI CCGC 1 cut(s) 231
AclWI GGATC 1 cut(s) 59
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 2 cut(s) 175, 218
AjnI CCWGG 1 cut(s) 310
AloI GAACNNNNNNTCC 2 cut(s) 21, 53
AluBI AGCT 2 cut(s) 237, 283
AluI AGCT 2 cut(s) 237, 283
AlwI GGATC 1 cut(s) 59
AoxI GGCC 1 cut(s) 15
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AseI ATTAAT 1 cut(s) 288
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 154
BanI GGYRCC 2 cut(s) 134, 314
BccI CCATC 2 cut(s) 20, 95
BciT130I CCWGG 1 cut(s) 312
Bme1390I CCNGG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 3 cut(s) 85, 136, 316
BmrFI CCNGG 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 1 cut(s) 80
Bse1I ACTGG 4 cut(s) 136, 273, 306, 318
Bse8I GATNNNNATC 1 cut(s) 50
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 138
BseJI GATNNNNATC 1 cut(s) 50
BseNI ACTGG 4 cut(s) 136, 273, 306, 318
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 2 cut(s) 134, 314
BsiSI CCGG 1 cut(s) 81
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 1 cut(s) 17
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 1 cut(s) 231
BspANI GGCC 1 cut(s) 17
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 3 cut(s) 85, 136, 316
BspPI GGATC 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 134, 314
BsrI ACTGG 4 cut(s) 136, 273, 306, 318
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 192, 235
BstDSI CCRYGG 1 cut(s) 138
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 1 cut(s) 54
BstMBI GATC 1 cut(s) 51
BstNI CCWGG 1 cut(s) 312
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 310
BstUI CGCG 1 cut(s) 158
BstXI CCANNNNNNTGG 1 cut(s) 325
BsuRI GGCC 1 cut(s) 17
BtgI CCRYGG 1 cut(s) 138
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 2 cut(s) 96, 266
Cac8I GCNNGC 2 cut(s) 192, 235
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 1 cut(s) 16
CviAII CATG 1 cut(s) 169
CviJI RGCY 5 cut(s) 17, 190, 237, 283, 305
CviKI_1 RGCY 5 cut(s) 17, 190, 237, 283, 305
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 310
FaeI CATG 1 cut(s) 172
FaiI YATR 3 cut(s) 170, 185, 279
FatI CATG 1 cut(s) 168
FauI CCCGC 1 cut(s) 238
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 1 cut(s) 152
HaeIII GGCC 1 cut(s) 17
HapII CCGG 1 cut(s) 81
HhaI GCGC 1 cut(s) 153
Hin1II CATG 1 cut(s) 172
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HinfI GANTC 2 cut(s) 30, 145
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 3 cut(s) 35, 45, 71
Hpy188III TCNNGA 1 cut(s) 23
Hpy8I GTNNAC 1 cut(s) 199
HpyAV CCTTC 1 cut(s) 7
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 1 cut(s) 10
Hsp92II CATG 1 cut(s) 172
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 1 cut(s) 51
MaeIII GTNAC 2 cut(s) 220, 264
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 2 cut(s) 42, 180
MseI TTAA 3 cut(s) 246, 288, 294
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 1 cut(s) 158
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 3 cut(s) 85, 136, 316
NmuCI GTSAC 1 cut(s) 264
NsbI TGCGCA 1 cut(s) 152
NspI RCATGY 1 cut(s) 172
PciI ACATGT 1 cut(s) 168
PctI GAATGC 1 cut(s) 65
PfeI GAWTC 2 cut(s) 30, 145
PscI ACATGT 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 3 cut(s) 85, 136, 316
PspPI GGNCC 1 cut(s) 16
SaqAI TTAA 3 cut(s) 246, 288, 294
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 312
SetI ASST 2 cut(s) 239, 285
SsiI CCGC 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 211
TfiI GAWTC 2 cut(s) 30, 145
Tru1I TTAA 3 cut(s) 246, 288, 294
Tru9I TTAA 3 cut(s) 246, 288, 294
TscAI CASTG 2 cut(s) 103, 273
TseFI GTSAC 1 cut(s) 264
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 2 cut(s) 90, 312
TspRI CASTG 2 cut(s) 103, 273
VspI ATTAAT 1 cut(s) 288
XceI RCATGY 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.