Rh2AG628400

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010660
Physical Location & Seq
Reverse (-)
85373830 .. 85375080
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG628400.1

Sequence Viewer

Length: 414 bp
ATGGCGGATACAAAACCAGCAAAGAGGAAACCTGTGTTCAAAAAGGTTATTGAGCTTCAGCCGGACACCAAGGGACACACTCTGATTGTAAAAGTGGTTAGTTCGAAGATGGTGTTGCAGAAGGGCCGTCCTGATGGGATCCAAGTTCGTCAGATGAAGATTGCTGAATGCTTGGTTGGAGACGAAACCGGAACCATCATTTTCACCGCTAGAAATGAACAAGTGGACTTGATGAAACCTGGTGCTACTCTGATTCTGAGGAATGCTAAAATTGACATGTTCAAGGGATCTATGAGGCTTGCTGTCGACAAATGGGGTCGAGTAGAAGTGACTGAACCAGTTAGCTTCACTGTCAGGGAAGATAACAACATGTCACTGGTCGAATATGAACTGGTTAGTGTTGTCGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.39

Weight (kDa)

9.41

Isoelectric Point (pI)

28.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 110 8.4e-46 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 306
AciI CCGC 2 cut(s) 5, 207
AclWI GGATC 3 cut(s) 133, 146, 295
AcuI CTGAAG 1 cut(s) 41
AflIII ACRYGT 2 cut(s) 276, 369
AgsI TTSAA 2 cut(s) 40, 283
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 2 cut(s) 55, 345
AluI AGCT 2 cut(s) 55, 345
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 3 cut(s) 133, 146, 295
AoxI GGCC 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 196
AsuII TTCGAA 1 cut(s) 104
BamHI GGATCC 1 cut(s) 138
BccI CCATC 3 cut(s) 103, 128, 203
BceAI ACGGC 1 cut(s) 111
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 210
Bme1390I CCNGG 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 124
BmiI GGNNCC 2 cut(s) 140, 193
BmrFI CCNGG 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 69
BsaWI WCCGGW 1 cut(s) 188
Bse1I ACTGG 3 cut(s) 338, 381, 396
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 3 cut(s) 338, 381, 396
BshFI GGCC 1 cut(s) 126
BsiSI CCGG 2 cut(s) 62, 189
BslFI GGGAC 1 cut(s) 87
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 2 cut(s) 173, 268
BsnI GGCC 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 104
Bsp143I GATC 2 cut(s) 138, 287
BspACI CCGC 2 cut(s) 5, 207
BspANI GGCC 1 cut(s) 126
BspCNI CTCAG 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 140, 193
BspPI GGATC 3 cut(s) 133, 146, 295
BspT104I TTCGAA 1 cut(s) 104
BsrI ACTGG 3 cut(s) 338, 381, 396
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 2 cut(s) 138, 287
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 352
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 1 cut(s) 257
BstKTI GATC 2 cut(s) 141, 290
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 2 cut(s) 138, 287
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 2 cut(s) 280, 373
BstSCI CCNGG 1 cut(s) 238
BstX2I RGATCY 2 cut(s) 138, 287
BstYI RGATCY 2 cut(s) 138, 287
BsuRI GGCC 1 cut(s) 126
BtsIMutI CAGTG 2 cut(s) 348, 374
Cac8I GCNNGC 1 cut(s) 300
Cfr13I GGNCC 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 238
CviAII CATG 2 cut(s) 277, 370
CviJI RGCY 5 cut(s) 55, 61, 126, 298, 345
CviKI_1 RGCY 5 cut(s) 55, 61, 126, 298, 345
DdeI CTNAG 1 cut(s) 257
DpnI GATC 2 cut(s) 140, 289
DpnII GATC 2 cut(s) 138, 287
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 2 cut(s) 280, 373
FaiI YATR 4 cut(s) 278, 293, 371, 387
FaqI GGGAC 1 cut(s) 87
FatI CATG 2 cut(s) 276, 369
FblI GTMKAC 1 cut(s) 306
FspBI CTAG 1 cut(s) 210
HaeIII GGCC 1 cut(s) 126
HapII CCGG 2 cut(s) 62, 189
Hin1II CATG 2 cut(s) 280, 373
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 1 cut(s) 253
HpaII CCGG 2 cut(s) 62, 189
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 2 cut(s) 226, 307
Hpy188I TCNGA 4 cut(s) 84, 153, 252, 258
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 2 cut(s) 226, 307
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 1 cut(s) 118
HpyF3I CTNAG 1 cut(s) 257
Hsp92II CATG 2 cut(s) 280, 373
Kzo9I GATC 2 cut(s) 138, 287
MabI ACCWGGT 1 cut(s) 238
MaeI CTAG 1 cut(s) 210
MaeIII GTNAC 2 cut(s) 328, 372
MalI GATC 2 cut(s) 140, 289
MboI GATC 2 cut(s) 138, 287
MboII GAAGA 3 cut(s) 118, 169, 371
MflI RGATCY 2 cut(s) 138, 287
MluCI AATT 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 157
MnlI CCTC 3 cut(s) 18, 252, 288
MspI CCGG 2 cut(s) 62, 189
MspR9I CCNGG 1 cut(s) 240
Mva1269I GAATGC 2 cut(s) 173, 268
MvaI CCWGG 1 cut(s) 240
NdeII GATC 2 cut(s) 138, 287
NlaIII CATG 2 cut(s) 280, 373
NlaIV GGNNCC 2 cut(s) 140, 193
NmuCI GTSAC 2 cut(s) 328, 372
NspI RCATGY 2 cut(s) 280, 373
NspV TTCGAA 1 cut(s) 104
PciI ACATGT 2 cut(s) 276, 369
PctI GAATGC 2 cut(s) 173, 268
PfeI GAWTC 1 cut(s) 253
PscI ACATGT 2 cut(s) 276, 369
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 140, 193
PspPI GGNCC 1 cut(s) 124
PsuI RGATCY 2 cut(s) 138, 287
SalI GTCGAC 1 cut(s) 305
Sau3AI GATC 2 cut(s) 138, 287
Sau96I GGNCC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 240
SetI ASST 5 cut(s) 34, 48, 57, 241, 347
SexAI ACCWGGT 1 cut(s) 238
SfuI TTCGAA 1 cut(s) 104
Sse9I AATT 1 cut(s) 270
SsiI CCGC 2 cut(s) 5, 207
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 352
TaqI TCGA 5 cut(s) 104, 306, 319, 381, 405
TasI AATT 1 cut(s) 270
TfiI GAWTC 1 cut(s) 253
TscAI CASTG 2 cut(s) 355, 381
TseFI GTSAC 2 cut(s) 328, 372
Tsp45I GTSAC 2 cut(s) 328, 372
TspDTI ATGAA 4 cut(s) 170, 231, 248, 402
TspRI CASTG 2 cut(s) 355, 381
XceI RCATGY 2 cut(s) 280, 373
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.