pycom17g03220

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010660
Physical Location & Seq
Forward (+)
2101927 .. 2103109
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g03220.3

Sequence Viewer

Length: 576 bp
ATGAAAATGTGGAAGATCAAACGGACGTTGAACCAATTTCATCTCTCTCTCTCTCTCTCTCTCTCTCTGGATCTGCAATTTGTTGTGTGCGTGTCTCTCTCTCTCTCTAAACGCTTTTTGTTAGAAATGGCGGAAACAAGACCGGCAAAGAGGAAACCTGTCTTCAAGAAGGTTGATCAGCTGAAACCGGACACCAAGGGACACACCTTGGTTGTGAAGGTTGTTAGTTCGAAGATGGTAATGCAGAAGGTCCGTCCTGATGGAATCCAAGTTCGTCAGATGAGGATCGCAGAATGCTTGGTTGGAGATGAAACCGGAACCATCATTTTCACTGCCAGAAATGATCAAGTTGACTTGATGACAACCGGTGCAACTGTAATCCTGCGCAACTCGAAAATAGACATGTTCAAGGGTTCTATGAGGCTTGCTGTGGACAAATGGGGTCGAGTTGAAGTAACCGAACCTGCCAGCTTCTCCGTGAAGGAAGATAACAACTTGTCACTAGTGGAATATGAGCTGATCAATGTTAACGAACTGGCTAATCCTGGTGCTGGTGAAGTGGTTAACGCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.44

Weight (kDa)

9.51

Isoelectric Point (pI)

17.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 472
AciI CCGC 1 cut(s) 131
AclWI GGATC 2 cut(s) 78, 293
AfiI CCNNNNNNNGG 1 cut(s) 551
AflIII ACRYGT 1 cut(s) 402
AgeI ACCGGT 1 cut(s) 365
AgsI TTSAA 4 cut(s) 31, 166, 409, 452
AhlI ACTAGT 1 cut(s) 502
AjnI CCWGG 1 cut(s) 544
AjuI GAANNNNNNNTTGG 2 cut(s) 285, 317
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
AluBI AGCT 3 cut(s) 181, 471, 517
AluI AGCT 3 cut(s) 181, 471, 517
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 2 cut(s) 78, 293
AsiGI ACCGGT 1 cut(s) 365
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 566
AsuII TTCGAA 1 cut(s) 230
AvaII GGWCC 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 154
BccI CCATC 3 cut(s) 229, 254, 329
BciT130I CCWGG 1 cut(s) 546
BclI TGATCA 3 cut(s) 175, 343, 519
BcoDI GTCTC 1 cut(s) 99
BcuI ACTAGT 1 cut(s) 502
BfaI CTAG 1 cut(s) 503
BfuAI ACCTGC 1 cut(s) 472
Bme1390I CCNGG 1 cut(s) 546
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 546
BpiI GAAGAC 1 cut(s) 154
Bpu14I TTCGAA 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 195, 207
BsaWI WCCGGW 3 cut(s) 187, 314, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 551
Bse118I RCCGGY 2 cut(s) 142, 365
Bse1I ACTGG 1 cut(s) 540
Bse8I GATNNNNATC 1 cut(s) 284
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 2 cut(s) 195, 207
BseJI GATNNNNATC 1 cut(s) 284
BseLI CCNNNNNNNGG 1 cut(s) 551
BseNI ACTGG 1 cut(s) 540
BshTI ACCGGT 1 cut(s) 365
BsiSI CCGG 4 cut(s) 143, 188, 315, 366
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 551
BsmAI GTCTC 1 cut(s) 99
BsmFI GGGAC 1 cut(s) 213
BsmI GAATGC 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 230
Bsp143I GATC 6 cut(s) 15, 70, 175, 285, 343, 519
BspACI CCGC 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 472
BspPI GGATC 2 cut(s) 78, 293
BspT104I TTCGAA 1 cut(s) 230
BsrFI RCCGGY 2 cut(s) 142, 365
BsrI ACTGG 1 cut(s) 540
BssAI RCCGGY 2 cut(s) 142, 365
BssECI CCNNGG 2 cut(s) 195, 207
BssMI GATC 6 cut(s) 15, 70, 175, 285, 343, 519
BssT1I CCWWGG 2 cut(s) 195, 207
Bst2UI CCWGG 1 cut(s) 546
Bst4CI ACNGT 1 cut(s) 376
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 2 cut(s) 426, 469
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 6 cut(s) 18, 73, 178, 288, 346, 522
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 6 cut(s) 15, 70, 175, 285, 343, 519
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 1 cut(s) 544
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 330
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 426, 469
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 2 cut(s) 142, 365
Cfr13I GGNCC 1 cut(s) 250
CspAI ACCGGT 1 cut(s) 365
CviAII CATG 1 cut(s) 403
CviJI RGCY 5 cut(s) 181, 424, 471, 517, 539
CviKI_1 RGCY 5 cut(s) 181, 424, 471, 517, 539
DpnI GATC 6 cut(s) 17, 72, 177, 287, 345, 521
DpnII GATC 6 cut(s) 15, 70, 175, 285, 343, 519
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 195, 207
Eco47I GGWCC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 544
EcoT14I CCWWGG 2 cut(s) 195, 207
ErhI CCWWGG 2 cut(s) 195, 207
FaeI CATG 1 cut(s) 406
FaiI YATR 3 cut(s) 404, 419, 513
FaqI GGGAC 1 cut(s) 213
FatI CATG 1 cut(s) 402
FbaI TGATCA 3 cut(s) 175, 343, 519
FspBI CTAG 1 cut(s) 503
FspI TGCGCA 1 cut(s) 386
GlaI GCGC 1 cut(s) 386
HapII CCGG 4 cut(s) 143, 188, 315, 366
HhaI GCGC 1 cut(s) 387
Hin1II CATG 1 cut(s) 406
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HincII GTYRAC 3 cut(s) 352, 529, 565
HindII GTYRAC 3 cut(s) 352, 529, 565
HinfI GANTC 1 cut(s) 264
HpaI GTTAAC 2 cut(s) 529, 565
HpaII CCGG 4 cut(s) 143, 188, 315, 366
HphI GGTGA 1 cut(s) 566
Hpy166II GTNNAC 4 cut(s) 352, 433, 529, 565
Hpy188I TCNGA 1 cut(s) 279
Hpy188III TCNNGA 3 cut(s) 68, 166, 257
Hpy8I GTNNAC 4 cut(s) 352, 433, 529, 565
HpyAV CCTTC 4 cut(s) 163, 211, 241, 475
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 3 cut(s) 76, 244, 371
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 1 cut(s) 406
HspAI GCGC 1 cut(s) 385
Ksp22I TGATCA 3 cut(s) 175, 343, 519
KspAI GTTAAC 2 cut(s) 529, 565
Kzo9I GATC 6 cut(s) 15, 70, 175, 285, 343, 519
MaeI CTAG 1 cut(s) 503
MaeII ACGT 1 cut(s) 26
MaeIII GTNAC 2 cut(s) 454, 498
MalI GATC 6 cut(s) 17, 72, 177, 287, 345, 521
MboI GATC 6 cut(s) 15, 70, 175, 285, 343, 519
MboII GAAGA 4 cut(s) 25, 154, 244, 497
MflI RGATCY 1 cut(s) 70
MluCI AATT 2 cut(s) 35, 77
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 3 cut(s) 144, 276, 414
MseI TTAA 2 cut(s) 528, 564
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 4 cut(s) 143, 188, 315, 366
MspR9I CCNGG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 1 cut(s) 546
NdeII GATC 6 cut(s) 15, 70, 175, 285, 343, 519
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 1 cut(s) 319
NmuCI GTSAC 1 cut(s) 498
NsbI TGCGCA 1 cut(s) 386
NspI RCATGY 1 cut(s) 406
NspV TTCGAA 1 cut(s) 230
PciI ACATGT 1 cut(s) 402
PctI GAATGC 1 cut(s) 299
PfeI GAWTC 1 cut(s) 264
PinAI ACCGGT 1 cut(s) 365
PscI ACATGT 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 544
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 1 cut(s) 250
PsuI RGATCY 1 cut(s) 70
PvuII CAGCTG 1 cut(s) 181
SaqAI TTAA 2 cut(s) 528, 564
Sau3AI GATC 6 cut(s) 15, 70, 175, 285, 343, 519
Sau96I GGNCC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 230
SinI GGWCC 1 cut(s) 250
SpeI ACTAGT 1 cut(s) 502
Sse9I AATT 2 cut(s) 35, 77
SsiI CCGC 1 cut(s) 131
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 1 cut(s) 544
StyI CCWWGG 2 cut(s) 195, 207
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 1 cut(s) 29
TaqI TCGA 3 cut(s) 230, 392, 445
TasI AATT 2 cut(s) 35, 77
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 2 cut(s) 528, 564
Tru9I TTAA 2 cut(s) 528, 564
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 498
Tsp45I GTSAC 1 cut(s) 498
TspDTI ATGAA 3 cut(s) 17, 29, 324
TspGWI ACGGA 3 cut(s) 37, 242, 466
TspRI CASTG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 250
XceI RCATGY 1 cut(s) 406
XspI CTAG 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.