Prupe.3G283900_v2.0.a1

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0010660
Physical Location & Seq
Reverse (-)
25769646 .. 25771927
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G283900.1

Sequence Viewer

Length: 456 bp
ATGGAGGAAACAAAACCAGCAAAGAGGAAACCCGTTTTCAAAAAGGTTGATCAGCTGAAACCAGACACCAAGGGACACACCCTGGTTGTAAAGGTTGTTAGTTCGAAGATGGTACTGCAGAAGGCCAGACCTGACGGGATCCAAGTTCGTCAGATGAGGATTGCTGAATGCTTGGTTGGTGATGAAACCGGAACCATCATTTTTACTGCCAGAAATGACCAAGTTGATTTGATGACGACTGGCGCTACAGTGATTCTGCGTAATGCAAAAATAGACATGTTCAAGGGTTCTATGAGACTTGCTGTGGACAAGTGGGGTCGAGTTGAAGTAACCGAACCAGCTAGCTTCGCTGTTAAGGAAGAGAACAACTTGTCGCTGGTAGAATATGAACTGATTGATGTTAATGAACTGGCTAATGCTGGTGCACGTGAAGTGGTTAGTGTTGTCGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.8

Weight (kDa)

8.66

Isoelectric Point (pI)

18.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 133, 146
AcvI CACGTG 1 cut(s) 428
AfaI GTAC 1 cut(s) 114
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 3 cut(s) 40, 283, 326
AjnI CCWGG 1 cut(s) 81
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 3 cut(s) 55, 341, 345
AluI AGCT 3 cut(s) 55, 341, 345
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 1 cut(s) 289
Alw44I GTGCAC 1 cut(s) 423
AlwI GGATC 2 cut(s) 133, 146
AoxI GGCC 1 cut(s) 123
ApaLI GTGCAC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 191
AsuII TTCGAA 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 341
BaeGI GKGCMC 1 cut(s) 427
BamHI GGATCC 1 cut(s) 138
BbrPI CACGTG 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 427
BccI CCATC 2 cut(s) 103, 203
BciT130I CCWGG 1 cut(s) 83
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 342
BfmI CTRYAG 2 cut(s) 116, 246
BfoI RGCGCY 1 cut(s) 246
Bme1390I CCNGG 1 cut(s) 83
BmiI GGNNCC 2 cut(s) 140, 193
BmrFI CCNGG 1 cut(s) 83
BmtI GCTAGC 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 104
BsaAI YACGTR 1 cut(s) 428
BsaJI CCNNGG 2 cut(s) 69, 81
BsaWI WCCGGW 1 cut(s) 188
Bse1I ACTGG 2 cut(s) 244, 414
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 2 cut(s) 69, 81
BseNI ACTGG 2 cut(s) 244, 414
BseSI GKGCMC 1 cut(s) 427
BshFI GGCC 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 427
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 87
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 1 cut(s) 173
BsnI GGCC 1 cut(s) 125
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 2 cut(s) 49, 138
BspANI GGCC 1 cut(s) 125
BspLI GGNNCC 2 cut(s) 140, 193
BspMAI CTGCAG 1 cut(s) 120
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 2 cut(s) 133, 146
BspT104I TTCGAA 1 cut(s) 104
BsrI ACTGG 2 cut(s) 244, 414
BssECI CCNNGG 2 cut(s) 69, 81
BssMI GATC 2 cut(s) 49, 138
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 354
BstBAI YACGTR 1 cut(s) 428
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 343
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 2 cut(s) 52, 141
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 2 cut(s) 49, 138
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 2 cut(s) 116, 246
BstSLI GKGCMC 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
BsuRI GGCC 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 343
CfoI GCGC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 113
CviAII CATG 1 cut(s) 277
CviJI RGCY 5 cut(s) 55, 125, 341, 345, 413
CviKI_1 RGCY 5 cut(s) 55, 125, 341, 345, 413
CviQI GTAC 1 cut(s) 113
DpnI GATC 2 cut(s) 51, 140
DpnII GATC 2 cut(s) 49, 138
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Eco130I CCWWGG 1 cut(s) 69
Eco72I CACGTG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 1 cut(s) 280
FaiI YATR 3 cut(s) 278, 293, 387
FaqI GGGAC 1 cut(s) 87
FatI CATG 1 cut(s) 276
FbaI TGATCA 1 cut(s) 49
FspBI CTAG 1 cut(s) 342
GlaI GCGC 1 cut(s) 244
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 189
HhaI GCGC 1 cut(s) 245
Hin1II CATG 1 cut(s) 280
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HinfI GANTC 1 cut(s) 253
HpaII CCGG 1 cut(s) 189
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 2 cut(s) 307, 425
Hpy188I TCNGA 1 cut(s) 153
Hpy8I GTNNAC 2 cut(s) 307, 425
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 3 cut(s) 118, 266, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 1 cut(s) 280
HspAI GCGC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 2 cut(s) 49, 138
MaeI CTAG 1 cut(s) 342
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 328
MalI GATC 2 cut(s) 51, 140
MboI GATC 2 cut(s) 49, 138
MboII GAAGA 2 cut(s) 118, 371
MflI RGATCY 1 cut(s) 138
MhlI GDGCHC 1 cut(s) 427
MnlI CCTC 2 cut(s) 18, 150
MseI TTAA 2 cut(s) 354, 402
MspA1I CMGCKG 1 cut(s) 55
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 173
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 347
NdeII GATC 2 cut(s) 49, 138
NheI GCTAGC 1 cut(s) 341
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 2 cut(s) 140, 193
NspI RCATGY 1 cut(s) 280
NspV TTCGAA 1 cut(s) 104
PciI ACATGT 1 cut(s) 276
PctI GAATGC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 253
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 428
PscI ACATGT 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 81
PspCI CACGTG 1 cut(s) 428
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 140, 193
PstI CTGCAG 1 cut(s) 120
PsuI RGATCY 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 55
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 354, 402
Sau3AI GATC 2 cut(s) 49, 138
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 1 cut(s) 427
SetI ASST 7 cut(s) 48, 57, 96, 133, 343, 347, 430
SfcI CTRYAG 2 cut(s) 116, 246
SfuI TTCGAA 1 cut(s) 104
SspMI CTAG 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 430
TaqI TCGA 3 cut(s) 104, 319, 447
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 2 cut(s) 354, 402
Tru9I TTAA 2 cut(s) 354, 402
TscAI CASTG 1 cut(s) 255
TspDTI ATGAA 3 cut(s) 198, 402, 420
TspRI CASTG 1 cut(s) 255
VneI GTGCAC 1 cut(s) 423
XceI RCATGY 1 cut(s) 280
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.