MD17G1034900.v1.1

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010660
Physical Location & Seq
Forward (+)
2514013 .. 2515431
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1034900.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGTAATGCAGAAGGCCCGTCCTGATGGAATCCAAGTTCGTCAGGTGAGGATCGCGGAATGCTTGGTTGGAGATGAAACCGGAACCATCATTTTCACTGCCAGAAATGATCAAGTTGACTTGATGACAACCGGTGCAACAGTAATCCTGCGCAACGCGAAAATAGACATGTTCAAGGGTTCTATGAGGCTTGCTGTGGACAAATGGGGTCGAGTTGAAGTAACCGAACCTGCCAGCTTCTCTGTGAAGGAAGATAACAACTTGTCACTAGTGGAATATGAGCTGATCAATGTTAATGAACTGGTTAATCCTGGTGCTGGTGAAGTGGTTAACGCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

12.42

Weight (kDa)

4.78

Isoelectric Point (pI)

12.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 4 - 74 1.1e-35 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 238
AccII CGCG 2 cut(s) 56, 158
AciI CCGC 1 cut(s) 56
AclWI GGATC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 317
AflIII ACRYGT 1 cut(s) 168
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 2 cut(s) 175, 218
AhlI ACTAGT 1 cut(s) 268
AjnI CCWGG 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AloI GAACNNNNNNTCC 2 cut(s) 21, 53
AluBI AGCT 2 cut(s) 237, 283
AluI AGCT 2 cut(s) 237, 283
AlwI GGATC 1 cut(s) 59
AoxI GGCC 1 cut(s) 15
AsiGI ACCGGT 1 cut(s) 131
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 2 cut(s) 58, 332
BccI CCATC 2 cut(s) 20, 95
BciT130I CCWGG 1 cut(s) 312
BclI TGATCA 2 cut(s) 109, 285
BcuI ACTAGT 1 cut(s) 268
BfaI CTAG 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 238
Bme1390I CCNGG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 1 cut(s) 312
BsaWI WCCGGW 2 cut(s) 80, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 306
BseBI CCWGG 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 317
BseNI ACTGG 1 cut(s) 306
Bsh1236I CGCG 2 cut(s) 56, 158
BshFI GGCC 1 cut(s) 17
BshTI ACCGGT 1 cut(s) 131
BsiSI CCGG 2 cut(s) 81, 132
BslI CCNNNNNNNGG 1 cut(s) 317
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 1 cut(s) 17
Bsp143I GATC 3 cut(s) 51, 109, 285
BspACI CCGC 1 cut(s) 56
BspANI GGCC 1 cut(s) 17
BspFNI CGCG 2 cut(s) 56, 158
BspLI GGNNCC 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 1 cut(s) 59
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 1 cut(s) 306
BssAI RCCGGY 1 cut(s) 131
BssMI GATC 3 cut(s) 51, 109, 285
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 192, 235
BstFNI CGCG 2 cut(s) 56, 158
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 3 cut(s) 54, 112, 288
BstMBI GATC 3 cut(s) 51, 109, 285
BstNI CCWGG 1 cut(s) 312
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 310
BstUI CGCG 2 cut(s) 56, 158
BsuRI GGCC 1 cut(s) 17
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 2 cut(s) 192, 235
CfoI GCGC 1 cut(s) 153
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 16
CspAI ACCGGT 1 cut(s) 131
CviAII CATG 1 cut(s) 169
CviJI RGCY 4 cut(s) 17, 190, 237, 283
CviKI_1 RGCY 4 cut(s) 17, 190, 237, 283
DpnI GATC 3 cut(s) 53, 111, 287
DpnII GATC 3 cut(s) 51, 109, 285
EcoRII CCWGG 1 cut(s) 310
FaeI CATG 1 cut(s) 172
FaiI YATR 3 cut(s) 170, 185, 279
FatI CATG 1 cut(s) 168
FbaI TGATCA 2 cut(s) 109, 285
FspBI CTAG 1 cut(s) 269
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 1 cut(s) 152
HaeIII GGCC 1 cut(s) 17
HapII CCGG 2 cut(s) 81, 132
HhaI GCGC 1 cut(s) 153
Hin1II CATG 1 cut(s) 172
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HincII GTYRAC 2 cut(s) 118, 331
HindII GTYRAC 2 cut(s) 118, 331
HinfI GANTC 1 cut(s) 30
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 2 cut(s) 81, 132
HphI GGTGA 2 cut(s) 58, 332
Hpy166II GTNNAC 3 cut(s) 118, 199, 331
Hpy188III TCNNGA 1 cut(s) 23
Hpy8I GTNNAC 3 cut(s) 118, 199, 331
HpyAV CCTTC 2 cut(s) 7, 241
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 2 cut(s) 10, 137
Hsp92II CATG 1 cut(s) 172
HspAI GCGC 1 cut(s) 151
Ksp22I TGATCA 2 cut(s) 109, 285
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 51, 109, 285
MaeI CTAG 1 cut(s) 269
MaeIII GTNAC 2 cut(s) 220, 264
MalI GATC 3 cut(s) 53, 111, 287
MboI GATC 3 cut(s) 51, 109, 285
MboII GAAGA 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 2 cut(s) 42, 180
MseI TTAA 3 cut(s) 294, 306, 330
MspI CCGG 2 cut(s) 81, 132
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 2 cut(s) 56, 158
NdeII GATC 3 cut(s) 51, 109, 285
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 264
NsbI TGCGCA 1 cut(s) 152
NspI RCATGY 1 cut(s) 172
PciI ACATGT 1 cut(s) 168
PctI GAATGC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 30
PinAI ACCGGT 1 cut(s) 131
PscI ACATGT 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 16
SaqAI TTAA 3 cut(s) 294, 306, 330
Sau3AI GATC 3 cut(s) 51, 109, 285
Sau96I GGNCC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 312
SetI ASST 4 cut(s) 48, 232, 239, 285
SpeI ACTAGT 1 cut(s) 268
SsiI CCGC 1 cut(s) 56
SspMI CTAG 1 cut(s) 269
StyD4I CCNGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 211
TfiI GAWTC 1 cut(s) 30
Tru1I TTAA 3 cut(s) 294, 306, 330
Tru9I TTAA 3 cut(s) 294, 306, 330
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 264
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 2 cut(s) 90, 312
TspRI CASTG 1 cut(s) 103
XceI RCATGY 1 cut(s) 172
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.