RLG00000022012

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010660
Physical Location & Seq
Reverse (-)
79968070 .. 79968653
584 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022012

Sequence Viewer

Length: 414 bp
ATGGCGGAGACAAAACCAGCAAAGAGGAAACCTGTGTTCAAAAAGGTTATTGAGCTTCAGCCGGACACCAAGGGACACACTCTGATTGTAAAAGTGGTTAGTTCGAACATGGTGTTGCAGAAGGGCCGGCCTGATGGGATCCAAGTTCGTCAGATGAAGATTGCTGAATGCTTGGTTGGAGACGAAACAGGAACCATCATTTTCACCGCTAGAAATGAACAAGTGGACTTGATGAAACCTGGTGCTACTCTGATTCTGAGGAATGCTAAAATTGACATGTTCAAGGGATCTATGAGGCTTGCTGTCGACAAATGGGGTCGAGTAGAAGTGACTGAACCAGCTAGCTTCACTGTCAGGGAAGATAACAACATGTCGCTGGTCGAATATGAACTGGTTAGTGTTGTTGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.36

Weight (kDa)

9.27

Isoelectric Point (pI)

27.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 110 1.1e-45 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 306
AciI CCGC 2 cut(s) 5, 207
AclWI GGATC 3 cut(s) 133, 146, 295
AcuI CTGAAG 1 cut(s) 41
AflIII ACRYGT 2 cut(s) 276, 369
AgsI TTSAA 3 cut(s) 40, 283, 407
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 3 cut(s) 55, 341, 345
AluI AGCT 3 cut(s) 55, 341, 345
Alw26I GTCTC 2 cut(s) 2, 174
AlwI GGATC 3 cut(s) 133, 146, 295
AoxI GGCC 2 cut(s) 124, 128
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 196
AsuII TTCGAA 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 341
BamHI GGATCC 1 cut(s) 138
BccI CCATC 2 cut(s) 128, 203
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 2 cut(s) 2, 174
BfaI CTAG 2 cut(s) 210, 342
Bme1390I CCNGG 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 124
BmiI GGNNCC 2 cut(s) 140, 193
BmrFI CCNGG 1 cut(s) 240
BmtI GCTAGC 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 69
Bse118I RCCGGY 1 cut(s) 126
Bse1I ACTGG 1 cut(s) 396
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 396
BshFI GGCC 2 cut(s) 126, 130
BsiSI CCGG 2 cut(s) 62, 127
BslFI GGGAC 1 cut(s) 87
BsmAI GTCTC 2 cut(s) 2, 174
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 2 cut(s) 173, 268
BsnI GGCC 2 cut(s) 126, 130
Bsp119I TTCGAA 1 cut(s) 104
Bsp143I GATC 2 cut(s) 138, 287
BspACI CCGC 2 cut(s) 5, 207
BspANI GGCC 2 cut(s) 126, 130
BspCNI CTCAG 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 140, 193
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 3 cut(s) 133, 146, 295
BspT104I TTCGAA 1 cut(s) 104
BsrFI RCCGGY 1 cut(s) 126
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 2 cut(s) 138, 287
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 352
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 3 cut(s) 128, 300, 343
BstDEI CTNAG 1 cut(s) 257
BstKTI GATC 2 cut(s) 141, 290
BstMAI GTCTC 2 cut(s) 2, 174
BstMBI GATC 2 cut(s) 138, 287
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 2 cut(s) 280, 373
BstSCI CCNGG 1 cut(s) 238
BstX2I RGATCY 2 cut(s) 138, 287
BstYI RGATCY 2 cut(s) 138, 287
BsuRI GGCC 2 cut(s) 126, 130
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 3 cut(s) 128, 300, 343
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 238
CviAII CATG 3 cut(s) 109, 277, 370
CviJI RGCY 7 cut(s) 55, 61, 126, 130, 298, 341, 345
CviKI_1 RGCY 7 cut(s) 55, 61, 126, 130, 298, 341, 345
DdeI CTNAG 1 cut(s) 257
DpnI GATC 2 cut(s) 140, 289
DpnII GATC 2 cut(s) 138, 287
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 3 cut(s) 112, 280, 373
FaiI YATR 5 cut(s) 110, 278, 293, 371, 387
FaqI GGGAC 1 cut(s) 87
FatI CATG 3 cut(s) 108, 276, 369
FblI GTMKAC 1 cut(s) 306
FseI GGCCGGCC 1 cut(s) 130
FspBI CTAG 2 cut(s) 210, 342
HaeIII GGCC 2 cut(s) 126, 130
HapII CCGG 2 cut(s) 62, 127
Hin1II CATG 3 cut(s) 112, 280, 373
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 1 cut(s) 253
HpaII CCGG 2 cut(s) 62, 127
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 2 cut(s) 226, 307
Hpy188I TCNGA 4 cut(s) 84, 153, 252, 258
Hpy8I GTNNAC 2 cut(s) 226, 307
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 1 cut(s) 118
HpyF3I CTNAG 1 cut(s) 257
Hsp92II CATG 3 cut(s) 112, 280, 373
KroI GCCGGC 1 cut(s) 126
KroNI GCCGGC 1 cut(s) 128
Kzo9I GATC 2 cut(s) 138, 287
MabI ACCWGGT 1 cut(s) 238
MaeI CTAG 2 cut(s) 210, 342
MaeIII GTNAC 1 cut(s) 328
MalI GATC 2 cut(s) 140, 289
MboI GATC 2 cut(s) 138, 287
MboII GAAGA 2 cut(s) 169, 371
MflI RGATCY 2 cut(s) 138, 287
MluCI AATT 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 157
MnlI CCTC 3 cut(s) 18, 252, 288
MroNI GCCGGC 1 cut(s) 126
MspI CCGG 2 cut(s) 62, 127
MspR9I CCNGG 1 cut(s) 240
Mva1269I GAATGC 2 cut(s) 173, 268
MvaI CCWGG 1 cut(s) 240
NaeI GCCGGC 1 cut(s) 128
NdeII GATC 2 cut(s) 138, 287
NgoMIV GCCGGC 1 cut(s) 126
NheI GCTAGC 1 cut(s) 341
NlaIII CATG 3 cut(s) 112, 280, 373
NlaIV GGNNCC 2 cut(s) 140, 193
NmuCI GTSAC 1 cut(s) 328
NspI RCATGY 2 cut(s) 280, 373
NspV TTCGAA 1 cut(s) 104
PciI ACATGT 2 cut(s) 276, 369
PctI GAATGC 2 cut(s) 173, 268
PdiI GCCGGC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 253
PscI ACATGT 2 cut(s) 276, 369
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 140, 193
PspPI GGNCC 1 cut(s) 124
PsuI RGATCY 2 cut(s) 138, 287
RigI GGCCGGCC 1 cut(s) 130
SalI GTCGAC 1 cut(s) 305
Sau3AI GATC 2 cut(s) 138, 287
Sau96I GGNCC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 240
SetI ASST 6 cut(s) 34, 48, 57, 241, 343, 347
SexAI ACCWGGT 1 cut(s) 238
SfuI TTCGAA 1 cut(s) 104
Sse9I AATT 1 cut(s) 270
SsiI CCGC 2 cut(s) 5, 207
SspMI CTAG 2 cut(s) 210, 342
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 352
TaqI TCGA 4 cut(s) 104, 306, 319, 381
TasI AATT 1 cut(s) 270
TfiI GAWTC 1 cut(s) 253
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 1 cut(s) 328
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 4 cut(s) 170, 231, 248, 402
TspRI CASTG 1 cut(s) 355
XceI RCATGY 2 cut(s) 280, 373
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 2 cut(s) 210, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.