FvH4_6g51290

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005843
Physical Location & Seq
Reverse (-)
38033865 .. 38038304
4440 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51290.t2

Sequence Viewer

Length: 1557 bp
ATGGCCGGACTAGAAGAACTGAGGAAAAAGCTCACACCTTTATTCGACGCAGAAAAAGGCCTGGGCTTTGGATCCTCCACTTTAGACCCCTCCGATTCCTACACATTATCGGACAGCGGAACTGTGAACTTGCTGAGCAGATCGTATGGGGTGTATAATATTAACGAGCTGGGACTGCAGAAGTGCACGTCTTCTACTACGGTGGACGATACCACAGAGAAGACTTACCAATGCGCGTCGCAGGAGATGAGGGTGTTTGGGGCGATTGGGAGTGGGGCTAGCAGTGTTGTGCAGAGAGCTATCCATATTCCCAATCATCGGATCATTGCGCTCAAGAAAATCAACATTTTTGAGAAGGAGAAAAGGCAGCAACTGCTTACTGAGATTAGGACATTATGTGAAGCACCTTGTTACCAAGGTCTTGTAGAGTTTCATGGGGCATTTTATACACCGGATTCTGGACAAATAAGCATAGCTTTGGAGTATATGGATGGAGGATCATTGGCTGATATCTTACGGTTGCAGAAAAGAATACCTGAACCCTTACTTTCTTCTATGTTTCAGAAGCTTCTACATGGACTGAGCTACTTGCATGGAGTTAGGTATCTAGTACACAGAGACATAAAACCTGCCAATTTGCTTATCAATCTCAAGGGCGAGCCAAAGATAACAGACTTCGGAATAAGTGCTGGGTTGGAGAATTCTATGGCAATGTGTGCAACATTTGTTGGGACAGTTACGTACATGTCACCTGAGCGAATTCGAAATGAGAATTATTCTTACCCAGCTGATATATGGAGCCTTGGGCTTGCTCTCTTTGAGTGTGGTACTGGAGAATTTCCATATACAGCTAATGACGGACCTGTCAACCTTATGTTACAGATCTTAGATGATCCATCACCTACTCCTTCAAAACAGAGTTTTTCACCAGAGTTCTGCTCGTTTATTGAAGCTTGCTTGCAGAAAGATGCAGATGCTAGGCCAACTGCAGAGCAGCTACTTGCCCACCCGTTCATTACAAAGTATGAGAATTCCCAATTGGACTTGGGAGCATTTGTTCGAAGTGTTTTTGATCCAACGCAAAGGATGAAGGACTTGGCAGATATGCTTACCATACATTATTACTTGCTCTTTGATGGACCTGATGAGCTCTGGCAACATGCAAAGACGTTATACAATGAAGACTCGGTTTTCAGTTTCTCTGGCAAGCAATTAGTAGGTGCGAATGAAATATTTGCCTCGCTCTCTAGTATACGAAGTACATTAGCTGGTGACTGGCCTCCTGAGAGGCTTGTGCATGTTGTGGAAAAGCTTCAATGCCGTGCTCATGGTCAAGATGGAGTAGCAATTAGGGTATCAGGATCCTTCATTGTTGGGAATCAGTTCCTTATATGTGGAGACGGTGTTCAGGTAGAGGGCCTGCCAAGTATTAAAGATCTTTCTATCGATATTTCGAGTAAGCGAATGGGTACATTCCGCGAGCAGTTCATTATGGAGCCAAGCAATATGATAGGGCGCTACTCCATAGCTAAACAAGAACTTTATATTTTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001101 GO:0001932 GO:0001934 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009753 GO:0009755 GO:0009791 GO:0009814 GO:0009864 GO:0009866 GO:0009867 GO:0009873 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033206 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04016 ko05418 map04016 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

519

Amino Acids

57.53

Weight (kDa)

5.34

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 86 - 336 1.4e-57 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 88 - 335 4.4e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 863
Acc36I ACCTGC 1 cut(s) 637
AccI GTMKAC 1 cut(s) 1252
AccII CGCG 2 cut(s) 236, 1479
AciI CCGC 2 cut(s) 117, 1477
AclWI GGATC 8 cut(s) 66, 79, 329, 505, 887, 1067, 1356, 1369
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 700, 759, 836, 1030
AfaI GTAC 5 cut(s) 612, 743, 829, 1261, 1471
AfiI CCNNNNNNNGG 2 cut(s) 318, 458
AflIII ACRYGT 1 cut(s) 744
AgsI TTSAA 3 cut(s) 912, 950, 1316
AjiI CACGTC 1 cut(s) 189
AjnI CCWGG 1 cut(s) 60
AjuI GAANNNNNNNTTGG 2 cut(s) 1022, 1054
AloI GAACNNNNNNTCC 4 cut(s) 1041, 1073, 1389, 1421
Alw21I GWGCWC 3 cut(s) 188, 1152, 1327
Alw26I GTCTC 2 cut(s) 612, 1392
Alw44I GTGCAC 1 cut(s) 184
AlwI GGATC 8 cut(s) 66, 79, 329, 505, 887, 1067, 1356, 1369
AlwNI CAGNNNCTG 1 cut(s) 373
AoxI GGCC 5 cut(s) 3, 58, 980, 1277, 1417
ApaLI GTGCAC 1 cut(s) 184
ApeKI GCWGC 2 cut(s) 367, 994
ApoI RAATTY 4 cut(s) 700, 759, 836, 1030
Asp700I GAANNNNTTC 2 cut(s) 1311, 1382
AspLEI GCGC 3 cut(s) 236, 331, 1518
AspS9I GGNCC 3 cut(s) 860, 1139, 1417
AsuHPI GGTGA 4 cut(s) 741, 891, 918, 1283
AsuII TTCGAA 2 cut(s) 763, 1060
AsuNHI GCTAGC 1 cut(s) 278
AvaII GGWCC 2 cut(s) 860, 1139
BaeGI GKGCMC 1 cut(s) 188
BamHI GGATCC 2 cut(s) 71, 1361
BanII GRGCYC 1 cut(s) 1152
BbsI GAAGAC 3 cut(s) 183, 227, 1188
Bbv12I GWGCWC 3 cut(s) 188, 1152, 1327
BbvI GCAGC 2 cut(s) 379, 1006
BccI CCATC 4 cut(s) 485, 904, 1130, 1331
BceAI ACGGC 1 cut(s) 1305
BciT130I CCWGG 1 cut(s) 62
BcoDI GTCTC 2 cut(s) 612, 1392
BfaI CTAG 5 cut(s) 11, 279, 608, 978, 1248
BfmI CTRYAG 2 cut(s) 176, 987
BfoI RGCGCY 1 cut(s) 1519
BfuAI ACCTGC 1 cut(s) 637
BglII AGATCT 2 cut(s) 882, 1435
BisI GCNGC 2 cut(s) 368, 995
BlpI GCTNAGC 1 cut(s) 134
BlsI GCNGC 2 cut(s) 369, 996
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 2 cut(s) 860, 1139
BmgBI CACGTC 1 cut(s) 189
BmgT120I GGNCC 3 cut(s) 860, 1139, 1417
BmiI GGNNCC 4 cut(s) 73, 800, 1363, 1497
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 2 cut(s) 958, 964
BmtI GCTAGC 1 cut(s) 282
BpiI GAAGAC 3 cut(s) 183, 227, 1188
BplI GAGNNNNNCTC 2 cut(s) 923, 955
BpmI CTGGAG 1 cut(s) 852
Bpu10I CCTNAGC 1 cut(s) 753
Bpu1102I GCTNAGC 1 cut(s) 134
Bpu14I TTCGAA 2 cut(s) 763, 1060
BpuEI CTTGAG 2 cut(s) 317, 635
Bsa29I ATCGAT 1 cut(s) 1446
BsaAI YACGTR 1 cut(s) 741
BsaJI CCNNGG 3 cut(s) 61, 415, 802
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 4 cut(s) 1041, 1071, 1389, 1419
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 458
Bse1I ACTGG 2 cut(s) 835, 1280
Bse3DI GCAATG 2 cut(s) 324, 717
BseBI CCWGG 1 cut(s) 62
BseCI ATCGAT 1 cut(s) 1446
BseDI CCNNGG 3 cut(s) 61, 415, 802
BseGI GGATG 2 cut(s) 496, 1092
BseLI CCNNNNNNNGG 2 cut(s) 318, 458
BseMI GCAATG 2 cut(s) 324, 717
BseMII CTCAG 6 cut(s) 11, 125, 372, 572, 744, 1275
BseNI ACTGG 2 cut(s) 835, 1280
BseSI GKGCMC 1 cut(s) 188
BseXI GCAGC 2 cut(s) 379, 1006
BseYI CCCAGC 3 cut(s) 169, 689, 784
BsgI GTGCAG 1 cut(s) 311
Bsh1236I CGCG 2 cut(s) 236, 1479
BshFI GGCC 5 cut(s) 5, 60, 982, 1279, 1419
BshVI ATCGAT 1 cut(s) 1446
BsiHKAI GWGCWC 3 cut(s) 188, 1152, 1327
BsiSI CCGG 2 cut(s) 6, 452
BslFI GGGAC 2 cut(s) 186, 745
BslI CCNNNNNNNGG 2 cut(s) 318, 458
BsmAI GTCTC 2 cut(s) 612, 1392
BsmBI CGTCTC 1 cut(s) 1392
BsmFI GGGAC 2 cut(s) 186, 745
BsnI GGCC 5 cut(s) 5, 60, 982, 1279, 1419
Bsp119I TTCGAA 2 cut(s) 763, 1060
Bsp1286I GDGCHC 3 cut(s) 188, 1152, 1327
Bsp143I GATC 9 cut(s) 71, 140, 321, 497, 882, 892, 1072, 1361, 1435
Bsp1720I GCTNAGC 1 cut(s) 134
BspACI CCGC 2 cut(s) 117, 1477
BspANI GGCC 5 cut(s) 5, 60, 982, 1279, 1419
BspCNI CTCAG 6 cut(s) 12, 126, 373, 573, 745, 1276
BspDI ATCGAT 1 cut(s) 1446
BspFNI CGCG 2 cut(s) 236, 1479
BspLI GGNNCC 4 cut(s) 73, 800, 1363, 1497
BspMAI CTGCAG 2 cut(s) 180, 991
BspMI ACCTGC 1 cut(s) 637
BspOI GCTAGC 1 cut(s) 282
BspPI GGATC 8 cut(s) 66, 79, 329, 505, 887, 1067, 1356, 1369
BspT104I TTCGAA 2 cut(s) 763, 1060
BsrDI GCAATG 2 cut(s) 324, 717
BsrI ACTGG 2 cut(s) 835, 1280
BssECI CCNNGG 3 cut(s) 61, 415, 802
BssMI GATC 9 cut(s) 71, 140, 321, 497, 882, 892, 1072, 1361, 1435
BssNAI GTATAC 1 cut(s) 1253
BssT1I CCWWGG 2 cut(s) 415, 802
Bst1107I GTATAC 1 cut(s) 1253
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 5 cut(s) 124, 202, 519, 736, 1403
BstAPI GCANNNNNTGC 2 cut(s) 373, 716
BstBAI YACGTR 1 cut(s) 741
BstBI TTCGAA 2 cut(s) 763, 1060
BstC8I GCNNGC 8 cut(s) 280, 659, 810, 955, 959, 1208, 1421, 1481
BstDEI CTNAG 7 cut(s) 20, 134, 381, 581, 753, 886, 1284
BstF5I GGATG 2 cut(s) 496, 1092
BstFNI CGCG 2 cut(s) 236, 1479
BstH2I RGCGCY 1 cut(s) 1519
BstHHI GCGC 3 cut(s) 236, 331, 1518
BstKTI GATC 9 cut(s) 74, 143, 324, 500, 885, 895, 1075, 1364, 1438
BstMAI GTCTC 2 cut(s) 612, 1392
BstMBI GATC 9 cut(s) 71, 140, 321, 497, 882, 892, 1072, 1361, 1435
BstMWI GCNNNNNNNGC 3 cut(s) 175, 373, 716
BstNI CCWGG 1 cut(s) 62
BstNSI RCATGY 3 cut(s) 748, 1163, 1301
BstSCI CCNGG 1 cut(s) 60
BstSFI CTRYAG 2 cut(s) 176, 987
BstSLI GKGCMC 1 cut(s) 188
BstSNI TACGTA 1 cut(s) 741
BstUI CGCG 2 cut(s) 236, 1479
BstV1I GCAGC 2 cut(s) 379, 1006
BstV2I GAAGAC 3 cut(s) 183, 227, 1188
BstX2I RGATCY 4 cut(s) 71, 882, 1361, 1435
BstYI RGATCY 4 cut(s) 71, 882, 1361, 1435
BstZ17I GTATAC 1 cut(s) 1253
Bsu15I ATCGAT 1 cut(s) 1446
BsuRI GGCC 5 cut(s) 5, 60, 982, 1279, 1419
BsuTUI ATCGAT 1 cut(s) 1446
BtrI CACGTC 1 cut(s) 189
BtsCI GGATG 2 cut(s) 496, 1092
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 289
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 8 cut(s) 280, 659, 810, 955, 959, 1208, 1421, 1481
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 3 cut(s) 236, 331, 1518
Cfr13I GGNCC 3 cut(s) 860, 1139, 1417
ClaI ATCGAT 1 cut(s) 1446
CseI GACGC 2 cut(s) 56, 225
Csp6I GTAC 5 cut(s) 611, 742, 828, 1260, 1470
CviAII CATG 7 cut(s) 434, 575, 593, 745, 1160, 1298, 1328
CviQI GTAC 5 cut(s) 611, 742, 828, 1260, 1470
DdeI CTNAG 7 cut(s) 20, 134, 381, 581, 753, 886, 1284
DpnI GATC 9 cut(s) 73, 142, 323, 499, 884, 894, 1074, 1363, 1437
DpnII GATC 9 cut(s) 71, 140, 321, 497, 882, 892, 1072, 1361, 1435
DrdI GACNNNNNNGTC 1 cut(s) 863
DseDI GACNNNNNNGTC 1 cut(s) 863
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 1150
Eco105I TACGTA 1 cut(s) 741
Eco130I CCWWGG 2 cut(s) 415, 802
Eco147I AGGCCT 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 1152
Eco32I GATATC 1 cut(s) 511
Eco47I GGWCC 2 cut(s) 860, 1139
Eco53kI GAGCTC 1 cut(s) 1150
EcoICRI GAGCTC 1 cut(s) 1150
EcoO109I RGGNCCY 1 cut(s) 1417
EcoRI GAATTC 3 cut(s) 700, 759, 1030
EcoRII CCWGG 1 cut(s) 60
EcoRV GATATC 1 cut(s) 511
EcoT14I CCWWGG 2 cut(s) 415, 802
EcoT38I GRGCYC 1 cut(s) 1152
ErhI CCWWGG 2 cut(s) 415, 802
Esp3I CGTCTC 1 cut(s) 1392
FaeI CATG 7 cut(s) 437, 578, 596, 748, 1163, 1301, 1331
FalI AAGNNNNNCTT 2 cut(s) 460, 492
FaqI GGGAC 2 cut(s) 186, 745
FatI CATG 7 cut(s) 433, 574, 592, 744, 1159, 1297, 1327
FblI GTMKAC 1 cut(s) 1252
Fnu4HI GCNGC 2 cut(s) 368, 995
FokI GGATG 2 cut(s) 503, 1099
FriOI GRGCYC 1 cut(s) 1152
Fsp4HI GCNGC 2 cut(s) 368, 995
FspBI CTAG 5 cut(s) 11, 279, 608, 978, 1248
GlaI GCGC 3 cut(s) 235, 330, 1517
GluI GCNGC 2 cut(s) 368, 995
GsaI CCCAGC 3 cut(s) 173, 693, 788
GsuI CTGGAG 1 cut(s) 852
HaeII RGCGCY 1 cut(s) 1519
HaeIII GGCC 5 cut(s) 5, 60, 982, 1279, 1419
HapII CCGG 2 cut(s) 6, 452
HgaI GACGC 2 cut(s) 56, 225
HhaI GCGC 3 cut(s) 236, 331, 1518
Hin1II CATG 7 cut(s) 437, 578, 596, 748, 1163, 1301, 1331
Hin6I GCGC 3 cut(s) 234, 329, 1516
HinP1I GCGC 3 cut(s) 234, 329, 1516
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HindIII AAGCTT 3 cut(s) 566, 951, 1310
HinfI GANTC 4 cut(s) 95, 455, 1184, 1378
HpaII CCGG 2 cut(s) 6, 452
HphI GGTGA 4 cut(s) 741, 891, 918, 1283
Hpy166II GTNNAC 6 cut(s) 127, 186, 205, 613, 868, 1253
Hpy188I TCNGA 5 cut(s) 94, 112, 321, 564, 680
Hpy188III TCNNGA 5 cut(s) 334, 459, 1283, 1334, 1359
Hpy8I GTNNAC 6 cut(s) 127, 186, 205, 613, 868, 1253
Hpy99I CGWCG 2 cut(s) 50, 241
HpyAV CCTTC 4 cut(s) 349, 918, 1084, 1375
HpyCH4III ACNGT 5 cut(s) 124, 202, 519, 736, 1403
HpyCH4IV ACGT 3 cut(s) 188, 740, 1169
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 373, 716
HpyF3I CTNAG 7 cut(s) 20, 134, 381, 581, 753, 886, 1284
HpySE526I ACGT 3 cut(s) 188, 740, 1169
Hsp92II CATG 7 cut(s) 437, 578, 596, 748, 1163, 1301, 1331
HspAI GCGC 3 cut(s) 234, 329, 1516
Kzo9I GATC 9 cut(s) 71, 140, 321, 497, 882, 892, 1072, 1361, 1435
LmnI GCTCC 3 cut(s) 798, 1049, 1495
Lsp1109I GCAGC 2 cut(s) 379, 1006
LweI GCATC 2 cut(s) 958, 964
MaeI CTAG 5 cut(s) 11, 279, 608, 978, 1248
MaeII ACGT 3 cut(s) 188, 740, 1169
MaeIII GTNAC 5 cut(s) 410, 736, 747, 876, 1271
MalI GATC 9 cut(s) 73, 142, 323, 499, 884, 894, 1074, 1363, 1437
MboI GATC 9 cut(s) 71, 140, 321, 497, 882, 892, 1072, 1361, 1435
MboII GAAGA 5 cut(s) 26, 183, 232, 543, 1193
MfeI CAATTG 1 cut(s) 1037
MflI RGATCY 4 cut(s) 71, 882, 1361, 1435
MhlI GDGCHC 3 cut(s) 188, 1152, 1327
MluCI AATT 9 cut(s) 634, 700, 759, 772, 836, 1030, 1037, 1211, 1347
MlyI GAGTC 1 cut(s) 1178
MmeI TCCRAC 2 cut(s) 675, 1100
MnlI CCTC 9 cut(s) 15, 85, 100, 243, 488, 1249, 1281, 1290, 1408
MroXI GAANNNNTTC 2 cut(s) 1311, 1382
MseI TTAA 2 cut(s) 162, 1431
MspA1I CMGCKG 2 cut(s) 117, 788
MspI CCGG 2 cut(s) 6, 452
MspR9I CCNGG 1 cut(s) 62
MunI CAATTG 1 cut(s) 1037
MvaI CCWGG 1 cut(s) 62
MvnI CGCG 2 cut(s) 236, 1479
MwoI GCNNNNNNNGC 3 cut(s) 175, 373, 716
NdeII GATC 9 cut(s) 71, 140, 321, 497, 882, 892, 1072, 1361, 1435
NheI GCTAGC 1 cut(s) 278
NlaIII CATG 7 cut(s) 437, 578, 596, 748, 1163, 1301, 1331
NlaIV GGNNCC 4 cut(s) 73, 800, 1363, 1497
NmuCI GTSAC 2 cut(s) 747, 1271
NspI RCATGY 3 cut(s) 748, 1163, 1301
NspV TTCGAA 2 cut(s) 763, 1060
PceI AGGCCT 1 cut(s) 60
PciI ACATGT 1 cut(s) 744
PdmI GAANNNNTTC 2 cut(s) 1311, 1382
PfeI GAWTC 3 cut(s) 95, 455, 1378
PkrI GCNGC 2 cut(s) 369, 996
PleI GAGTC 1 cut(s) 1178
PpsI GAGTC 1 cut(s) 1178
Ppu21I YACGTR 1 cut(s) 741
PscI ACATGT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 1152
Psp6I CCWGG 1 cut(s) 60
PspFI CCCAGC 3 cut(s) 169, 689, 784
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 4 cut(s) 73, 800, 1363, 1497
PspPI GGNCC 3 cut(s) 860, 1139, 1417
PstI CTGCAG 2 cut(s) 180, 991
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 4 cut(s) 71, 882, 1361, 1435
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 5 cut(s) 612, 743, 829, 1261, 1471
RsaNI GTAC 5 cut(s) 611, 742, 828, 1260, 1470
SacI GAGCTC 1 cut(s) 1152
SaqAI TTAA 2 cut(s) 162, 1431
SatI GCNGC 2 cut(s) 368, 995
Sau3AI GATC 9 cut(s) 71, 140, 321, 497, 882, 892, 1072, 1361, 1435
Sau96I GGNCC 3 cut(s) 860, 1139, 1417
SchI GAGTC 1 cut(s) 1178
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 3 cut(s) 188, 1152, 1327
SfaNI GCATC 2 cut(s) 958, 964
SfcI CTRYAG 2 cut(s) 176, 987
SfuI TTCGAA 2 cut(s) 763, 1060
SinI GGWCC 2 cut(s) 860, 1139
SmlI CTYRAG 2 cut(s) 332, 650
SmoI CTYRAG 2 cut(s) 332, 650
SnaBI TACGTA 1 cut(s) 741
Sse9I AATT 9 cut(s) 634, 700, 759, 772, 836, 1030, 1037, 1211, 1347
SseBI AGGCCT 1 cut(s) 60
SsiI CCGC 2 cut(s) 117, 1477
SspI AATATT 2 cut(s) 160, 1233
SspMI CTAG 5 cut(s) 11, 279, 608, 978, 1248
SstI GAGCTC 1 cut(s) 1152
StuI AGGCCT 1 cut(s) 60
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 2 cut(s) 415, 802
TaaI ACNGT 5 cut(s) 124, 202, 519, 736, 1403
TaiI ACGT 3 cut(s) 191, 743, 1172
TaqI TCGA 5 cut(s) 45, 763, 1060, 1446, 1454
TasI AATT 9 cut(s) 634, 700, 759, 772, 836, 1030, 1037, 1211, 1347
TatI WGTACW 2 cut(s) 610, 1259
TfiI GAWTC 3 cut(s) 95, 455, 1378
Tru1I TTAA 2 cut(s) 162, 1431
Tru9I TTAA 2 cut(s) 162, 1431
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 2 cut(s) 747, 1271
TseI GCWGC 2 cut(s) 367, 994
Tsp45I GTSAC 2 cut(s) 747, 1271
TspDTI ATGAA 7 cut(s) 422, 1003, 1103, 1194, 1242, 1357, 1477
TspGWI ACGGA 1 cut(s) 873
TspRI CASTG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 184
VpaK11BI GGWCC 2 cut(s) 860, 1139
XapI RAATTY 4 cut(s) 700, 759, 836, 1030
XceI RCATGY 3 cut(s) 748, 1163, 1301
XcmI CCANNNNNNNNNTGG 1 cut(s) 792
XmiI GTMKAC 1 cut(s) 1252
XmnI GAANNNNTTC 2 cut(s) 1311, 1382
XspI CTAG 5 cut(s) 11, 279, 608, 978, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.