pycom111g02190

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0005843
Physical Location & Seq
Reverse (-)
1316893 .. 1319489
2597 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02190.3

Sequence Viewer

Length: 1560 bp
ATGGCTGGATTAGAAGAACTTAGAAAAAAGCTCACTCCTTTGTTCGACGCCGAAAAGGGCTTCTCCGCCGGCGGCCCTTCCTTGGAGCTCGACCCTTGCGATTCCTACACTCTATCGGACAGTGGAACGGTGAACTTGCTGAGCAGATCGTACGGCGTGTACAACATCAATGAGCTCGGATTGCAGAAATGCACGACCTTGGCGGTGGACGACAGCGCCGAGAAGACCTACCGGTGCGCCTCGCGGGAGATGAGGGTGTTTGGGGCAATCGGCAGCGGCGCCAGCAGTGTTGTGCAGAGAGCTATCCACATTCCCACTCATCGGATTCTAGCTCTCAAGAAGATCAACATTTTCGAGAAGGAGAAAAGGCAACAGCTGCTTACTGAGATACGAACACTATGCGAAGCACCTTGTTACCAAGGCCTTGTAGAATTTCACGGCGCATTTTATACACCGGATTCTGGACAAATAAGCATAGCTTTGGAGTATATGGATGGAGGATCGTTGGCGGATGTATTAAGATTGCAGAAAAGAATACCCGAACCTCTACTTTCTTCTATGTTTCAAAAGCTTCTACATGGTCTGAGGTACTTGCACGGAGTTAGACATCTAGTCCACAGAGACATAAAGCCTGCCAATTTGCTTGTGAATCTGAAGGGTGAGCCAAAAATAACAGATTTTGGTATAAGTGCTGGCTTAGAGAATTCAATGGCAATGTGTGCGACATTTGTTGGGACGGTCACATACATGTCGCCTGAGAGAATTCGAAACGAGAATTATTCTTATCCAGCTGATATTTGGAGCCTTGGTCTTGCTCTCTTTGAGTGTGGTACTGGAGAATTCCCGTATACAGCTACTGAAGGACCTGTCAATCTTATGCTGCAGATCCTGGATGATCCATCACCTACACCTTCAAAACACAAATTTTCACCAGAGTTCTGCTCGTTTATTGAAGCTTGCTTGCGAAAGGATCAAGATGCTAGGCCAACAGCAGAGCAGCTACTTTTACACCCCTTCATCACAAAGTACGAGGATTCCCAAGTGGACTTAGCAGCATTTGTCCGAAGCGTATTTGATCCAACACAAAGGATGAAGGACTTGGCAGATATGCTTACGATACATTACTACCTACTATTTGATGGACCTGATGAGCAATGGCAACATACAAGGACGTTATACAATGAAGATTCAGTTTTCAGTTTCTCAGGAAAGCAGCATGTCGGCCCAAATGATATCTTTGCGACTCTATCAAGTATCCGGAGTACACTAGCTGGTGACTGGCCTCCTGAAAGACTTGTGCACGTTGTAGAAAAGCTTCAGTGCCGCGCCCACGGTCAGGATGGAGTGGCCATTAGGGTATCCGGATCATTCATTGTTGGGAATCAGTTCCTCATATGTGGAGATGGCGTACAAGTGGAGGGCTTGCCAAGCATCAAAGATCTTTCCATTGATATTTCGAGTAAGCGAATGGGTACGTTTCAGGAGCAGTTCATCATGGAGCCGAGCAACATAATTGGTTGCTACTCCATAGCTAAACAAGAACTTTACATTATGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001101 GO:0001932 GO:0001934 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009753 GO:0009755 GO:0009791 GO:0009814 GO:0009864 GO:0009866 GO:0009867 GO:0009873 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033206 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04016 ko05418 map04016 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

520

Amino Acids

57.89

Weight (kDa)

5.66

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 848
AccII CGCG 2 cut(s) 244, 1326
AccIII TCCGGA 2 cut(s) 1257, 1361
AciI CCGC 7 cut(s) 66, 72, 203, 244, 276, 509, 1324
AclWI GGATC 6 cut(s) 508, 880, 890, 978, 1070, 1372
AcoI YGGCCR 1 cut(s) 1347
AcsI RAATTY 5 cut(s) 431, 703, 762, 839, 923
AcuI CTGAAG 3 cut(s) 674, 879, 1301
AcyI GRCGYC 2 cut(s) 48, 279
AfaI GTAC 8 cut(s) 152, 161, 590, 832, 1028, 1264, 1410, 1474
AfiI CCNNNNNNNGG 4 cut(s) 82, 321, 461, 1336
AflIII ACRYGT 1 cut(s) 747
AgeI ACCGGT 1 cut(s) 231
AgsI TTSAA 4 cut(s) 566, 708, 915, 953
AhdI GACNNNNNGTC 1 cut(s) 611
AjnI CCWGG 1 cut(s) 888
Alw21I GWGCWC 3 cut(s) 90, 177, 1302
Alw26I GTCTC 1 cut(s) 615
Alw44I GTGCAC 1 cut(s) 1298
AlwI GGATC 6 cut(s) 508, 880, 890, 978, 1070, 1372
AlwNI CAGNNNCTG 2 cut(s) 857, 889
Aor13HI TCCGGA 2 cut(s) 1257, 1361
AoxI GGCC 6 cut(s) 73, 421, 983, 1222, 1280, 1347
ApaLI GTGCAC 1 cut(s) 1298
ApeKI GCWGC 6 cut(s) 273, 376, 880, 997, 1052, 1213
ApoI RAATTY 5 cut(s) 431, 703, 762, 839, 923
AsiGI ACCGGT 1 cut(s) 231
Asp700I GAANNNNTTC 2 cut(s) 1314, 1385
AspLEI GCGC 5 cut(s) 218, 239, 281, 443, 1328
AspS9I GGNCC 4 cut(s) 74, 863, 1142, 1223
AsuHPI GGTGA 5 cut(s) 142, 671, 894, 921, 1286
AsuII TTCGAA 1 cut(s) 766
AvaII GGWCC 2 cut(s) 863, 1142
BaeGI GKGCMC 1 cut(s) 1302
BalI TGGCCA 1 cut(s) 1349
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 2 cut(s) 90, 177
BarI GAAGNNNNNNTAC 2 cut(s) 1299, 1331
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 3 cut(s) 90, 177, 1302
BbvI GCAGC 6 cut(s) 285, 363, 867, 1009, 1064, 1225
BccI CCATC 5 cut(s) 488, 907, 1133, 1334, 1397
BceAI ACGGC 2 cut(s) 169, 454
BcgI CGANNNNNNTGC 2 cut(s) 381, 415
BciT130I CCWGG 1 cut(s) 890
BciVI GTATCC 2 cut(s) 1265, 1369
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 4 cut(s) 329, 611, 981, 1268
BfmI CTRYAG 1 cut(s) 881
BfoI RGCGCY 2 cut(s) 219, 282
BfuI GTATCC 2 cut(s) 1265, 1369
BglII AGATCT 1 cut(s) 1438
BisI GCNGC 9 cut(s) 73, 274, 277, 377, 881, 998, 1053, 1214, 1324
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 9 cut(s) 74, 275, 278, 378, 882, 999, 1054, 1215, 1325
Bme1390I CCNGG 1 cut(s) 890
Bme18I GGWCC 2 cut(s) 863, 1142
BmeRI GACNNNNNGTC 1 cut(s) 611
BmgT120I GGNCC 4 cut(s) 74, 863, 1142, 1223
BmiI GGNNCC 3 cut(s) 280, 803, 1500
BmrFI CCNGG 1 cut(s) 890
BmsI GCATC 2 cut(s) 967, 1440
BpiI GAAGAC 1 cut(s) 230
BplI GAGNNNNNCTC 2 cut(s) 926, 958
BpmI CTGGAG 1 cut(s) 855
Bpu1102I GCTNAGC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 766
BpuEI CTTGAG 1 cut(s) 320
BsaHI GRCGYC 2 cut(s) 48, 279
BsaJI CCNNGG 5 cut(s) 81, 198, 418, 805, 1330
BsaWI WCCGGW 4 cut(s) 231, 454, 1257, 1361
BsaXI ACNNNNNCTCC 4 cut(s) 793, 823, 1392, 1422
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 321, 461, 1336
Bse118I RCCGGY 2 cut(s) 68, 231
Bse1I ACTGG 2 cut(s) 838, 1283
Bse3DI GCAATG 2 cut(s) 720, 1160
BseAI TCCGGA 2 cut(s) 1257, 1361
BseBI CCWGG 1 cut(s) 890
BseDI CCNNGG 5 cut(s) 81, 198, 418, 805, 1330
BseGI GGATG 5 cut(s) 499, 517, 898, 1095, 1345
BseLI CCNNNNNNNGG 4 cut(s) 82, 321, 461, 1336
BseMI GCAATG 2 cut(s) 720, 1160
BseMII CTCAG 5 cut(s) 131, 375, 575, 747, 1218
BseNI ACTGG 2 cut(s) 838, 1283
BseSI GKGCMC 1 cut(s) 1302
BseXI GCAGC 6 cut(s) 285, 363, 867, 1009, 1064, 1225
BsgI GTGCAG 1 cut(s) 314
Bsh1236I CGCG 2 cut(s) 244, 1326
BshFI GGCC 6 cut(s) 75, 423, 985, 1224, 1282, 1349
BshNI GGYRCC 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 231
BsiHKAI GWGCWC 3 cut(s) 90, 177, 1302
BsiSI CCGG 5 cut(s) 69, 232, 455, 1258, 1362
BsiWI CGTACG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 748
BslI CCNNNNNNNGG 4 cut(s) 82, 321, 461, 1336
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 748
BsnI GGCC 6 cut(s) 75, 423, 985, 1224, 1282, 1349
Bsp119I TTCGAA 1 cut(s) 766
Bsp1286I GDGCHC 3 cut(s) 90, 177, 1302
Bsp13I TCCGGA 2 cut(s) 1257, 1361
Bsp1407I TGTACA 1 cut(s) 159
Bsp143I GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 7 cut(s) 66, 72, 203, 244, 276, 509, 1324
BspANI GGCC 6 cut(s) 75, 423, 985, 1224, 1282, 1349
BspCNI CTCAG 5 cut(s) 132, 376, 576, 748, 1217
BspEI TCCGGA 2 cut(s) 1257, 1361
BspFNI CGCG 2 cut(s) 244, 1326
BspLI GGNNCC 3 cut(s) 280, 803, 1500
BspMAI CTGCAG 1 cut(s) 885
BspPI GGATC 6 cut(s) 508, 880, 890, 978, 1070, 1372
BspT104I TTCGAA 1 cut(s) 766
BspT107I GGYRCC 1 cut(s) 278
BsrDI GCAATG 2 cut(s) 720, 1160
BsrFI RCCGGY 2 cut(s) 68, 231
BsrGI TGTACA 1 cut(s) 159
BsrI ACTGG 2 cut(s) 838, 1283
BssAI RCCGGY 2 cut(s) 68, 231
BssECI CCNNGG 5 cut(s) 81, 198, 418, 805, 1330
BssMI GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
BssNAI GTATAC 1 cut(s) 849
BssNI GRCGYC 2 cut(s) 48, 279
BssT1I CCWWGG 4 cut(s) 81, 198, 418, 805
Bst1107I GTATAC 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 890
Bst4CI ACNGT 4 cut(s) 122, 130, 739, 1334
BstACI GRCGYC 2 cut(s) 48, 279
BstAPI GCANNNNNTGC 2 cut(s) 376, 719
BstAUI TGTACA 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 766
BstC8I GCNNGC 7 cut(s) 70, 283, 633, 694, 958, 962, 1424
BstDEI CTNAG 8 cut(s) 20, 140, 384, 584, 697, 756, 1048, 1204
BstDSI CCRYGG 1 cut(s) 1330
BstF5I GGATG 5 cut(s) 499, 517, 898, 1095, 1345
BstFNI CGCG 2 cut(s) 244, 1326
BstH2I RGCGCY 2 cut(s) 219, 282
BstHHI GCGC 5 cut(s) 218, 239, 281, 443, 1328
BstKTI GATC 9 cut(s) 149, 345, 503, 888, 898, 973, 1078, 1367, 1441
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
BstMWI GCNNNNNNNGC 5 cut(s) 181, 282, 376, 719, 1428
BstNI CCWGG 1 cut(s) 890
BstNSI RCATGY 2 cut(s) 751, 1220
BstSCI CCNGG 1 cut(s) 888
BstSFI CTRYAG 1 cut(s) 881
BstSLI GKGCMC 1 cut(s) 1302
BstUI CGCG 2 cut(s) 244, 1326
BstV1I GCAGC 6 cut(s) 285, 363, 867, 1009, 1064, 1225
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 2 cut(s) 885, 1438
BstYI RGATCY 2 cut(s) 885, 1438
BstZ17I GTATAC 1 cut(s) 849
BsuI GTATCC 2 cut(s) 1265, 1369
BsuRI GGCC 6 cut(s) 75, 423, 985, 1224, 1282, 1349
BtgI CCRYGG 1 cut(s) 1330
BtsCI GGATG 5 cut(s) 499, 517, 898, 1095, 1345
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 3 cut(s) 127, 292, 1325
Cac8I GCNNGC 7 cut(s) 70, 283, 633, 694, 958, 962, 1424
CaiI CAGNNNCTG 2 cut(s) 857, 889
CfoI GCGC 5 cut(s) 218, 239, 281, 443, 1328
Cfr10I RCCGGY 2 cut(s) 68, 231
Cfr13I GGNCC 4 cut(s) 74, 863, 1142, 1223
CseI GACGC 1 cut(s) 56
Csp6I GTAC 8 cut(s) 151, 160, 589, 831, 1027, 1263, 1409, 1473
CspAI ACCGGT 1 cut(s) 231
CviAII CATG 4 cut(s) 578, 748, 1217, 1495
CviQI GTAC 8 cut(s) 151, 160, 589, 831, 1027, 1263, 1409, 1473
DdeI CTNAG 8 cut(s) 20, 140, 384, 584, 697, 756, 1048, 1204
DinI GGCGCC 1 cut(s) 280
DpnI GATC 9 cut(s) 148, 344, 502, 887, 897, 972, 1077, 1366, 1440
DpnII GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
DriI GACNNNNNGTC 1 cut(s) 611
EaeI YGGCCR 1 cut(s) 1347
Eam1105I GACNNNNNGTC 1 cut(s) 611
EciI GGCGGA 2 cut(s) 55, 524
Ecl136II GAGCTC 2 cut(s) 88, 175
Eco130I CCWWGG 4 cut(s) 81, 198, 418, 805
Eco147I AGGCCT 1 cut(s) 423
Eco24I GRGCYC 2 cut(s) 90, 177
Eco32I GATATC 1 cut(s) 1234
Eco47I GGWCC 2 cut(s) 863, 1142
Eco53kI GAGCTC 2 cut(s) 88, 175
Eco57I CTGAAG 3 cut(s) 674, 879, 1301
EcoICRI GAGCTC 2 cut(s) 88, 175
EcoO109I RGGNCCY 1 cut(s) 863
EcoRI GAATTC 3 cut(s) 703, 762, 839
EcoRII CCWGG 1 cut(s) 888
EcoRV GATATC 1 cut(s) 1234
EcoT14I CCWWGG 4 cut(s) 81, 198, 418, 805
EcoT38I GRGCYC 2 cut(s) 90, 177
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 4 cut(s) 81, 198, 418, 805
FaeI CATG 4 cut(s) 581, 751, 1220, 1498
FalI AAGNNNNNCTT 2 cut(s) 463, 495
FaqI GGGAC 1 cut(s) 748
FatI CATG 4 cut(s) 577, 747, 1216, 1494
FauI CCCGC 1 cut(s) 237
FauNDI CATATG 1 cut(s) 1394
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 9 cut(s) 73, 274, 277, 377, 881, 998, 1053, 1214, 1324
FokI GGATG 5 cut(s) 506, 524, 905, 1102, 1352
FriOI GRGCYC 2 cut(s) 90, 177
Fsp4HI GCNGC 9 cut(s) 73, 274, 277, 377, 881, 998, 1053, 1214, 1324
FspBI CTAG 4 cut(s) 329, 611, 981, 1268
GlaI GCGC 5 cut(s) 217, 238, 280, 442, 1327
GluI GCNGC 9 cut(s) 73, 274, 277, 377, 881, 998, 1053, 1214, 1324
GsuI CTGGAG 1 cut(s) 855
HaeII RGCGCY 2 cut(s) 219, 282
HaeIII GGCC 6 cut(s) 75, 423, 985, 1224, 1282, 1349
HapII CCGG 5 cut(s) 69, 232, 455, 1258, 1362
HgaI GACGC 1 cut(s) 56
HhaI GCGC 5 cut(s) 218, 239, 281, 443, 1328
Hin1I GRCGYC 2 cut(s) 48, 279
Hin1II CATG 4 cut(s) 581, 751, 1220, 1498
Hin6I GCGC 5 cut(s) 216, 237, 279, 441, 1326
HinP1I GCGC 5 cut(s) 216, 237, 279, 441, 1326
HindIII AAGCTT 3 cut(s) 569, 954, 1313
HinfI GANTC 8 cut(s) 101, 325, 458, 649, 1034, 1187, 1243, 1381
HpaII CCGG 5 cut(s) 69, 232, 455, 1258, 1362
HphI GGTGA 5 cut(s) 142, 671, 894, 921, 1286
Hpy166II GTNNAC 8 cut(s) 133, 160, 208, 616, 849, 1045, 1265, 1300
Hpy188I TCNGA 6 cut(s) 118, 179, 324, 585, 654, 1064
Hpy8I GTNNAC 8 cut(s) 133, 160, 208, 616, 849, 1045, 1265, 1300
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 7 cut(s) 87, 352, 649, 854, 921, 1024, 1087
HpyCH4III ACNGT 4 cut(s) 122, 130, 739, 1334
HpyCH4IV ACGT 3 cut(s) 1172, 1302, 1475
HpyCH4V TGCA 7 cut(s) 184, 192, 295, 526, 595, 883, 1300
HpyF10VI GCNNNNNNNGC 5 cut(s) 181, 282, 376, 719, 1428
HpyF3I CTNAG 8 cut(s) 20, 140, 384, 584, 697, 756, 1048, 1204
HpySE526I ACGT 3 cut(s) 1172, 1302, 1475
Hsp92I GRCGYC 2 cut(s) 48, 279
Hsp92II CATG 4 cut(s) 581, 751, 1220, 1498
HspAI GCGC 5 cut(s) 216, 237, 279, 441, 1326
KasI GGCGCC 1 cut(s) 278
Kpn2I TCCGGA 2 cut(s) 1257, 1361
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Kzo9I GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
LmnI GCTCC 4 cut(s) 85, 801, 1483, 1498
Lsp1109I GCAGC 6 cut(s) 285, 363, 867, 1009, 1064, 1225
LweI GCATC 2 cut(s) 967, 1440
MaeI CTAG 4 cut(s) 329, 611, 981, 1268
MaeII ACGT 3 cut(s) 1172, 1302, 1475
MaeIII GTNAC 3 cut(s) 413, 739, 1274
MalI GATC 9 cut(s) 148, 344, 502, 887, 897, 972, 1077, 1366, 1440
MboI GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
MboII GAAGA 5 cut(s) 26, 235, 352, 546, 1196
MflI RGATCY 2 cut(s) 885, 1438
MhlI GDGCHC 3 cut(s) 90, 177, 1302
MlsI TGGCCA 1 cut(s) 1349
MluCI AATT 8 cut(s) 431, 637, 703, 762, 775, 839, 923, 1512
MluNI TGGCCA 1 cut(s) 1349
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 1 cut(s) 1237
MmeI TCCRAC 1 cut(s) 1103
MnlI CCTC 9 cut(s) 246, 250, 491, 555, 579, 1024, 1293, 1400, 1411
Mox20I TGGCCA 1 cut(s) 1349
MreI CGCCGGCG 1 cut(s) 68
MroI TCCGGA 2 cut(s) 1257, 1361
MroNI GCCGGC 1 cut(s) 68
MroXI GAANNNNTTC 2 cut(s) 1314, 1385
MscI TGGCCA 1 cut(s) 1349
MseI TTAA 1 cut(s) 518
MslI CAYNNNNRTG 1 cut(s) 746
Msp20I TGGCCA 1 cut(s) 1349
MspA1I CMGCKG 3 cut(s) 276, 376, 791
MspI CCGG 5 cut(s) 69, 232, 455, 1258, 1362
MspR9I CCNGG 1 cut(s) 890
MvaI CCWGG 1 cut(s) 890
MvnI CGCG 2 cut(s) 244, 1326
MwoI GCNNNNNNNGC 5 cut(s) 181, 282, 376, 719, 1428
NaeI GCCGGC 1 cut(s) 70
NarI GGCGCC 1 cut(s) 279
NdeI CATATG 1 cut(s) 1394
NdeII GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 4 cut(s) 581, 751, 1220, 1498
NlaIV GGNNCC 3 cut(s) 280, 803, 1500
NmeAIII GCCGAG 2 cut(s) 244, 1527
NmuCI GTSAC 2 cut(s) 739, 1274
NspI RCATGY 2 cut(s) 751, 1220
NspV TTCGAA 1 cut(s) 766
PceI AGGCCT 1 cut(s) 423
PciI ACATGT 1 cut(s) 747
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 216
PdiI GCCGGC 1 cut(s) 70
PdmI GAANNNNTTC 2 cut(s) 1314, 1385
PfeI GAWTC 7 cut(s) 101, 325, 458, 649, 1034, 1187, 1381
Pfl23II CGTACG 1 cut(s) 150
PfoI TCCNGGA 1 cut(s) 888
PinAI ACCGGT 1 cut(s) 231
PkrI GCNGC 9 cut(s) 74, 275, 278, 378, 882, 999, 1054, 1215, 1325
PleI GAGTC 1 cut(s) 1237
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 1237
PpuMI RGGWCCY 1 cut(s) 863
PscI ACATGT 1 cut(s) 747
Psp124BI GAGCTC 2 cut(s) 90, 177
Psp5II RGGWCCY 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 888
PspGI CCWGG 1 cut(s) 888
PspLI CGTACG 1 cut(s) 150
PspN4I GGNNCC 3 cut(s) 280, 803, 1500
PspPI GGNCC 4 cut(s) 74, 863, 1142, 1223
PspPPI RGGWCCY 1 cut(s) 863
PstI CTGCAG 1 cut(s) 885
PstNI CAGNNNCTG 2 cut(s) 857, 889
PsuI RGATCY 2 cut(s) 885, 1438
PvuII CAGCTG 2 cut(s) 376, 791
RsaI GTAC 8 cut(s) 152, 161, 590, 832, 1028, 1264, 1410, 1474
RsaNI GTAC 8 cut(s) 151, 160, 589, 831, 1027, 1263, 1409, 1473
RseI CAYNNNNRTG 1 cut(s) 746
SacI GAGCTC 2 cut(s) 90, 177
SaqAI TTAA 1 cut(s) 518
SatI GCNGC 9 cut(s) 73, 274, 277, 377, 881, 998, 1053, 1214, 1324
Sau3AI GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
Sau96I GGNCC 4 cut(s) 74, 863, 1142, 1223
SchI GAGTC 1 cut(s) 1237
ScrFI CCNGG 1 cut(s) 890
SduI GDGCHC 3 cut(s) 90, 177, 1302
SfaNI GCATC 2 cut(s) 967, 1440
SfcI CTRYAG 1 cut(s) 881
SfoI GGCGCC 1 cut(s) 280
SfuI TTCGAA 1 cut(s) 766
SgrAI CRCCGGYG 1 cut(s) 68
SinI GGWCC 2 cut(s) 863, 1142
SmiMI CAYNNNNRTG 1 cut(s) 746
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 8 cut(s) 431, 637, 703, 762, 775, 839, 923, 1512
SseBI AGGCCT 1 cut(s) 423
SsiI CCGC 7 cut(s) 66, 72, 203, 244, 276, 509, 1324
SspDI GGCGCC 1 cut(s) 278
SspMI CTAG 4 cut(s) 329, 611, 981, 1268
SstI GAGCTC 2 cut(s) 90, 177
StuI AGGCCT 1 cut(s) 423
StyD4I CCNGG 1 cut(s) 888
StyI CCWWGG 4 cut(s) 81, 198, 418, 805
TaaI ACNGT 4 cut(s) 122, 130, 739, 1334
TaiI ACGT 3 cut(s) 1175, 1305, 1478
TaqI TCGA 5 cut(s) 45, 90, 354, 766, 1457
TasI AATT 8 cut(s) 431, 637, 703, 762, 775, 839, 923, 1512
TatI WGTACW 2 cut(s) 159, 1262
TauI GCSGC 3 cut(s) 75, 279, 1326
TfiI GAWTC 7 cut(s) 101, 325, 458, 649, 1034, 1187, 1381
Tru1I TTAA 1 cut(s) 518
Tru9I TTAA 1 cut(s) 518
TscAI CASTG 3 cut(s) 127, 292, 1325
TseFI GTSAC 2 cut(s) 739, 1274
TseI GCWGC 6 cut(s) 273, 376, 880, 997, 1052, 1213
Tsp45I GTSAC 2 cut(s) 739, 1274
TspDTI ATGAA 5 cut(s) 1006, 1106, 1197, 1360, 1480
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 3 cut(s) 127, 292, 1325
VneI GTGCAC 1 cut(s) 1298
VpaK11BI GGWCC 2 cut(s) 863, 1142
XapI RAATTY 5 cut(s) 431, 703, 762, 839, 923
XceI RCATGY 2 cut(s) 751, 1220
XcmI CCANNNNNNNNNTGG 2 cut(s) 795, 1337
XmiI GTMKAC 1 cut(s) 848
XmnI GAANNNNTTC 2 cut(s) 1314, 1385
XspI CTAG 4 cut(s) 329, 611, 981, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.