Prupe.3G290900_v2.0.a1

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0005843
Physical Location & Seq
Forward (+)
26090347 .. 26093889
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G290900.1

Sequence Viewer

Length: 1557 bp
ATGGCTGGCTTAGAGGAATTAAGAAAAAAGCTCACGCCTTTGTTCGATGCCGAAAAGGGCTTCTCCACGGGCTCAACCTTGGACTTGGACCCTTGCGATTCTTACACGCTATCGGACAGTGGAACGGTCAACTTGCTGAGCAGATCGTACGGTGTGTACAACATCAATGAGCTCGGATTGCAGAAATGCACGACCTTGGCGGTGGACGACAGCAGCGAAAAGACGTACCGGTGTGGTTCGCATGAGATGAGGGTATTTGGGGCAATTGGCAGTGGCGCCAGCAGCGTTGTACAGAGAGCTATCCACATTCCCACTCATCGGATTTTAGCGCTCAAGAAGATCAATATTTTTGAAAAGGAGAAAAGGCAACAGCTGCTTACTGAGATACGGACGCTATGTGAAGCACCTTGCTACCAAGGTCTTGTAGAATTTCATGGTGCATTTTATACGCCGGATTCTGGTCAAATAAGCATTGCTCTGGAGTATATGGATGGAGGATCATTGGCGGATATCTTAAGATTGCGGAAAAGAATACCAGAACCTCTACTTTCTTCTATGTTTCAAAAGCTTCTGCATGGGCTGAGCTACTTGCATGGAGTTAGGCATCTAGTCCACCGAGACATAAAGCCTGCCAATTTGCTTGTAAATCTCAAGGGTGAGCCAAAAATAACAGATTTTGGTATAAGTGCTGGCTTAGAGAATTCAATGGCAATGTGTGCGACATTCGTTGGGACAGTTACATACATGTCGCCTGAGAGAATTCGAAATGAGAATTATTCTTATCCAGCTGATATTTGGAGCCTTGGTCTTGCTCTCTTTGAGTGTGGTACCGGTGAATTCCCATATACAGCTAATGAAGGACCTGTCAATCTTATGTTGCAGATCTTGGATGATCCATCACCTACGCCTTCAAAACACAATTTTTCACCAGAGTTTTGCTCGTTTATTGAAGCCTGTTTGCAAAAGGATGCAGATGCTAGGCCAACAGCAGAGCAGCTACTTTCACACCCCTTCATTACAAAGTATGAGGATTCCCAAGTGGACTTAGCAGTGTTTGTCCGAAGCGTATTTGATCCAACACAAAGGATGAAGGACTTGGCAGATATGCTTACAATACATTTTTACCTACTTTTTGATGGACCTGATGAGCTCTGGCAACATACAAGGACCTTATACAGTGAAGATTCAGTTTTCAGTTTCTCAGGAAAGCAACTAGTCGGCCCAAATGATATCTTTGCAAGTCTGTCAAGTATACGAAGTACATTAGCTGGTGACTGGCCTCCTGAAAGACTTGTGCATGTTGTGGAAAAGCTTCAATGCCGTGCTCATGGTCAGGATGGTGTAGCAATTAGGGTATCCGGATCATTCATTGTCGGGAATCAGTTCCTTATATGTGGAGACGGTGTACAGGTAGAGGGCTTGCCAAGCATTAAAGATCTTTCCATTGATATTTCGAGTAAGCGAATGGGTACATTCCGGGAGCAGTTCATTATGGAGCCAAGCAATATAATAGGGCGCTACTCCATATCTAAACAAGAACTTTACATTATGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001101 GO:0001932 GO:0001934 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009753 GO:0009755 GO:0009791 GO:0009814 GO:0009864 GO:0009866 GO:0009867 GO:0009873 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033206 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04016 ko05418 map04016 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

519

Amino Acids

57.73

Weight (kDa)

5.51

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 827
AccB1I GGYRCC 2 cut(s) 275, 827
AccI GTMKAC 1 cut(s) 1252
AccIII TCCGGA 1 cut(s) 1358
AciI CCGC 3 cut(s) 200, 506, 523
AclWI GGATC 4 cut(s) 505, 887, 1067, 1369
AcsI RAATTY 4 cut(s) 428, 700, 759, 836
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 8 cut(s) 149, 158, 227, 291, 829, 1261, 1407, 1471
AfeI AGCGCT 1 cut(s) 330
AfiI CCNNNNNNNGG 2 cut(s) 318, 458
AflII CTTAAG 1 cut(s) 514
AflIII ACRYGT 1 cut(s) 744
AgeI ACCGGT 2 cut(s) 228, 830
AgsI TTSAA 6 cut(s) 353, 563, 705, 912, 950, 1316
AhlI ACTAGT 1 cut(s) 1213
Alw21I GWGCWC 3 cut(s) 174, 1152, 1327
Alw26I GTCTC 2 cut(s) 612, 1392
AlwI GGATC 4 cut(s) 505, 887, 1067, 1369
Aor13HI TCCGGA 1 cut(s) 1358
Aor51HI AGCGCT 1 cut(s) 330
AoxI GGCC 3 cut(s) 980, 1219, 1277
ApeKI GCWGC 4 cut(s) 213, 282, 373, 994
ApoI RAATTY 4 cut(s) 428, 700, 759, 836
AsiGI ACCGGT 2 cut(s) 228, 830
Asp700I GAANNNNTTC 2 cut(s) 1311, 1382
Asp718I GGTACC 1 cut(s) 827
AspLEI GCGC 3 cut(s) 278, 331, 1518
AspS9I GGNCC 5 cut(s) 88, 860, 1139, 1167, 1220
AsuC2I CCSGG 1 cut(s) 1478
AsuHPI GGTGA 5 cut(s) 668, 845, 891, 918, 1283
AsuII TTCGAA 1 cut(s) 763
AvaII GGWCC 4 cut(s) 88, 860, 1139, 1167
BanI GGYRCC 2 cut(s) 275, 827
BanII GRGCYC 3 cut(s) 74, 174, 1152
Bbv12I GWGCWC 3 cut(s) 174, 1152, 1327
BbvI GCAGC 4 cut(s) 225, 294, 360, 1006
BccI CCATC 4 cut(s) 485, 904, 1130, 1331
BceAI ACGGC 1 cut(s) 1305
BciVI GTATCC 1 cut(s) 1366
BcnI CCSGG 1 cut(s) 1478
BcoDI GTCTC 2 cut(s) 612, 1392
BcuI ACTAGT 1 cut(s) 1213
BfaI CTAG 3 cut(s) 608, 978, 1214
BfoI RGCGCY 3 cut(s) 279, 332, 1519
BfrI CTTAAG 1 cut(s) 514
BfuI GTATCC 1 cut(s) 1366
BglII AGATCT 2 cut(s) 882, 1435
BisI GCNGC 4 cut(s) 214, 283, 374, 995
BlpI GCTNAGC 2 cut(s) 137, 581
BlsI GCNGC 4 cut(s) 215, 284, 375, 996
Bme1390I CCNGG 1 cut(s) 1478
Bme18I GGWCC 4 cut(s) 88, 860, 1139, 1167
BmgT120I GGNCC 5 cut(s) 88, 860, 1139, 1167, 1220
BmiI GGNNCC 5 cut(s) 90, 277, 800, 829, 1497
BmrFI CCNGG 1 cut(s) 1478
BmsI GCATC 4 cut(s) 37, 613, 958, 964
BplI GAGNNNNNCTC 2 cut(s) 923, 955
BpmI CTGGAG 1 cut(s) 500
Bpu1102I GCTNAGC 2 cut(s) 137, 581
Bpu14I TTCGAA 1 cut(s) 763
BpuEI CTTGAG 2 cut(s) 317, 635
BpuMI CCSGG 1 cut(s) 1478
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 5 cut(s) 66, 78, 195, 415, 802
BsaWI WCCGGW 3 cut(s) 228, 830, 1358
BsaXI ACNNNNNCTCC 4 cut(s) 790, 820, 1389, 1419
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 458
Bse118I RCCGGY 2 cut(s) 228, 830
Bse1I ACTGG 1 cut(s) 1280
Bse3DI GCAATG 2 cut(s) 471, 717
BseAI TCCGGA 1 cut(s) 1358
BseDI CCNNGG 5 cut(s) 66, 78, 195, 415, 802
BseGI GGATG 5 cut(s) 496, 895, 973, 1092, 1342
BseLI CCNNNNNNNGG 2 cut(s) 318, 458
BseMI GCAATG 2 cut(s) 471, 717
BseMII CTCAG 5 cut(s) 128, 372, 572, 744, 1215
BseNI ACTGG 1 cut(s) 1280
BseXI GCAGC 4 cut(s) 225, 294, 360, 1006
BshFI GGCC 3 cut(s) 982, 1221, 1279
BshNI GGYRCC 2 cut(s) 275, 827
BshTI ACCGGT 2 cut(s) 228, 830
BsiHKAI GWGCWC 3 cut(s) 174, 1152, 1327
BsiSI CCGG 5 cut(s) 229, 452, 831, 1359, 1477
BsiWI CGTACG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 745
BslI CCNNNNNNNGG 2 cut(s) 318, 458
BsmAI GTCTC 2 cut(s) 612, 1392
BsmBI CGTCTC 1 cut(s) 1392
BsmFI GGGAC 1 cut(s) 745
BsnI GGCC 3 cut(s) 982, 1221, 1279
Bsp119I TTCGAA 1 cut(s) 763
Bsp1286I GDGCHC 4 cut(s) 74, 174, 1152, 1327
Bsp13I TCCGGA 1 cut(s) 1358
Bsp1407I TGTACA 3 cut(s) 156, 289, 1405
Bsp143I GATC 8 cut(s) 143, 339, 497, 882, 892, 1072, 1361, 1435
Bsp1720I GCTNAGC 2 cut(s) 137, 581
BspACI CCGC 3 cut(s) 200, 506, 523
BspANI GGCC 3 cut(s) 982, 1221, 1279
BspCNI CTCAG 5 cut(s) 129, 373, 573, 745, 1214
BspEI TCCGGA 1 cut(s) 1358
BspLI GGNNCC 5 cut(s) 90, 277, 800, 829, 1497
BspPI GGATC 4 cut(s) 505, 887, 1067, 1369
BspT104I TTCGAA 1 cut(s) 763
BspT107I GGYRCC 2 cut(s) 275, 827
BspTI CTTAAG 1 cut(s) 514
BsrDI GCAATG 2 cut(s) 471, 717
BsrFI RCCGGY 2 cut(s) 228, 830
BsrGI TGTACA 3 cut(s) 156, 289, 1405
BsrI ACTGG 1 cut(s) 1280
BssAI RCCGGY 2 cut(s) 228, 830
BssECI CCNNGG 5 cut(s) 66, 78, 195, 415, 802
BssMI GATC 8 cut(s) 143, 339, 497, 882, 892, 1072, 1361, 1435
BssNAI GTATAC 1 cut(s) 1253
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 4 cut(s) 78, 195, 415, 802
Bst1107I GTATAC 1 cut(s) 1253
Bst4CI ACNGT 6 cut(s) 119, 127, 152, 736, 1178, 1403
BstACI GRCGYC 1 cut(s) 276
BstAFI CTTAAG 1 cut(s) 514
BstAPI GCANNNNNTGC 2 cut(s) 373, 716
BstAUI TGTACA 3 cut(s) 156, 289, 1405
BstBI TTCGAA 1 cut(s) 763
BstC8I GCNNGC 5 cut(s) 7, 280, 630, 691, 1421
BstDEI CTNAG 8 cut(s) 10, 137, 381, 581, 694, 753, 1045, 1201
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 5 cut(s) 496, 895, 973, 1092, 1342
BstH2I RGCGCY 3 cut(s) 279, 332, 1519
BstHHI GCGC 3 cut(s) 278, 331, 1518
BstKTI GATC 8 cut(s) 146, 342, 500, 885, 895, 1075, 1364, 1438
BstMAI GTCTC 2 cut(s) 612, 1392
BstMBI GATC 8 cut(s) 143, 339, 497, 882, 892, 1072, 1361, 1435
BstMWI GCNNNNNNNGC 5 cut(s) 178, 282, 373, 716, 1425
BstNSI RCATGY 2 cut(s) 748, 1301
BstSCI CCNGG 1 cut(s) 1476
BstV1I GCAGC 4 cut(s) 225, 294, 360, 1006
BstX2I RGATCY 2 cut(s) 882, 1435
BstYI RGATCY 2 cut(s) 882, 1435
BstZ17I GTATAC 1 cut(s) 1253
BsuI GTATCC 1 cut(s) 1366
BsuRI GGCC 3 cut(s) 982, 1221, 1279
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 5 cut(s) 496, 895, 973, 1092, 1342
BtsI GCAGTG 2 cut(s) 277, 1056
BtsIMutI CAGTG 4 cut(s) 124, 277, 1056, 1183
Cac8I GCNNGC 5 cut(s) 7, 280, 630, 691, 1421
CfoI GCGC 3 cut(s) 278, 331, 1518
Cfr10I RCCGGY 2 cut(s) 228, 830
Cfr13I GGNCC 5 cut(s) 88, 860, 1139, 1167, 1220
CseI GACGC 1 cut(s) 400
Csp6I GTAC 8 cut(s) 148, 157, 226, 290, 828, 1260, 1406, 1470
CspAI ACCGGT 2 cut(s) 228, 830
CviAII CATG 7 cut(s) 242, 434, 575, 593, 745, 1298, 1328
CviQI GTAC 8 cut(s) 148, 157, 226, 290, 828, 1260, 1406, 1470
DdeI CTNAG 8 cut(s) 10, 137, 381, 581, 694, 753, 1045, 1201
DinI GGCGCC 1 cut(s) 277
DpnI GATC 8 cut(s) 145, 341, 499, 884, 894, 1074, 1363, 1437
DpnII GATC 8 cut(s) 143, 339, 497, 882, 892, 1072, 1361, 1435
EciI GGCGGA 1 cut(s) 521
Ecl136II GAGCTC 2 cut(s) 172, 1150
Eco130I CCWWGG 4 cut(s) 78, 195, 415, 802
Eco24I GRGCYC 3 cut(s) 74, 174, 1152
Eco32I GATATC 2 cut(s) 511, 1231
Eco47I GGWCC 4 cut(s) 88, 860, 1139, 1167
Eco47III AGCGCT 1 cut(s) 330
Eco53kI GAGCTC 2 cut(s) 172, 1150
EcoICRI GAGCTC 2 cut(s) 172, 1150
EcoO109I RGGNCCY 2 cut(s) 860, 1167
EcoRI GAATTC 3 cut(s) 700, 759, 836
EcoRV GATATC 2 cut(s) 511, 1231
EcoT14I CCWWGG 4 cut(s) 78, 195, 415, 802
EcoT38I GRGCYC 3 cut(s) 74, 174, 1152
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
ErhI CCWWGG 4 cut(s) 78, 195, 415, 802
Esp3I CGTCTC 1 cut(s) 1392
FaeI CATG 7 cut(s) 245, 437, 578, 596, 748, 1301, 1331
FaqI GGGAC 1 cut(s) 745
FatI CATG 7 cut(s) 241, 433, 574, 592, 744, 1297, 1327
FblI GTMKAC 1 cut(s) 1252
Fnu4HI GCNGC 4 cut(s) 214, 283, 374, 995
FokI GGATG 5 cut(s) 503, 902, 980, 1099, 1349
FriOI GRGCYC 3 cut(s) 74, 174, 1152
Fsp4HI GCNGC 4 cut(s) 214, 283, 374, 995
FspBI CTAG 3 cut(s) 608, 978, 1214
GlaI GCGC 3 cut(s) 277, 330, 1517
GluI GCNGC 4 cut(s) 214, 283, 374, 995
GsuI CTGGAG 1 cut(s) 500
HaeII RGCGCY 3 cut(s) 279, 332, 1519
HaeIII GGCC 3 cut(s) 982, 1221, 1279
HapII CCGG 5 cut(s) 229, 452, 831, 1359, 1477
HgaI GACGC 1 cut(s) 400
HhaI GCGC 3 cut(s) 278, 331, 1518
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 7 cut(s) 245, 437, 578, 596, 748, 1301, 1331
Hin6I GCGC 3 cut(s) 276, 329, 1516
HinP1I GCGC 3 cut(s) 276, 329, 1516
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 2 cut(s) 566, 1310
HinfI GANTC 5 cut(s) 98, 455, 1031, 1184, 1378
HpaII CCGG 5 cut(s) 229, 452, 831, 1359, 1477
HphI GGTGA 5 cut(s) 668, 845, 891, 918, 1283
Hpy166II GTNNAC 7 cut(s) 130, 157, 205, 613, 1042, 1253, 1406
Hpy188I TCNGA 4 cut(s) 115, 176, 321, 1061
Hpy188III TCNNGA 7 cut(s) 334, 479, 1203, 1283, 1334, 1359, 1375
Hpy8I GTNNAC 7 cut(s) 130, 157, 205, 613, 1042, 1253, 1406
HpyAV CCTTC 4 cut(s) 851, 918, 1021, 1084
HpyCH4III ACNGT 6 cut(s) 119, 127, 152, 736, 1178, 1403
HpyCH4IV ACGT 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 5 cut(s) 178, 282, 373, 716, 1425
HpyF3I CTNAG 8 cut(s) 10, 137, 381, 581, 694, 753, 1045, 1201
HpySE526I ACGT 1 cut(s) 224
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 7 cut(s) 245, 437, 578, 596, 748, 1301, 1331
HspAI GCGC 3 cut(s) 276, 329, 1516
KasI GGCGCC 1 cut(s) 275
Kpn2I TCCGGA 1 cut(s) 1358
KpnI GGTACC 1 cut(s) 831
Kzo9I GATC 8 cut(s) 143, 339, 497, 882, 892, 1072, 1361, 1435
LmnI GCTCC 3 cut(s) 798, 1480, 1495
Lsp1109I GCAGC 4 cut(s) 225, 294, 360, 1006
LweI GCATC 4 cut(s) 37, 613, 958, 964
MaeI CTAG 3 cut(s) 608, 978, 1214
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 2 cut(s) 736, 1271
MalI GATC 8 cut(s) 145, 341, 499, 884, 894, 1074, 1363, 1437
MboI GATC 8 cut(s) 143, 339, 497, 882, 892, 1072, 1361, 1435
MboII GAAGA 3 cut(s) 349, 543, 1193
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 2 cut(s) 882, 1435
MhlI GDGCHC 4 cut(s) 74, 174, 1152, 1327
Mly113I GGCGCC 1 cut(s) 276
MmeI TCCRAC 1 cut(s) 1100
MnlI CCTC 7 cut(s) 7, 243, 488, 552, 1021, 1290, 1408
MroI TCCGGA 1 cut(s) 1358
MroXI GAANNNNTTC 2 cut(s) 1311, 1382
MseI TTAA 3 cut(s) 20, 515, 1431
MspA1I CMGCKG 2 cut(s) 373, 788
MspCI CTTAAG 1 cut(s) 514
MspI CCGG 5 cut(s) 229, 452, 831, 1359, 1477
MspR9I CCNGG 1 cut(s) 1478
MunI CAATTG 1 cut(s) 264
MwoI GCNNNNNNNGC 5 cut(s) 178, 282, 373, 716, 1425
NarI GGCGCC 1 cut(s) 276
NciI CCSGG 1 cut(s) 1478
NdeII GATC 8 cut(s) 143, 339, 497, 882, 892, 1072, 1361, 1435
NlaIII CATG 7 cut(s) 245, 437, 578, 596, 748, 1301, 1331
NlaIV GGNNCC 5 cut(s) 90, 277, 800, 829, 1497
NmuCI GTSAC 1 cut(s) 1271
NspI RCATGY 2 cut(s) 748, 1301
NspV TTCGAA 1 cut(s) 763
PciI ACATGT 1 cut(s) 744
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PdmI GAANNNNTTC 2 cut(s) 1311, 1382
PfeI GAWTC 5 cut(s) 98, 455, 1031, 1184, 1378
Pfl23II CGTACG 1 cut(s) 147
PfoI TCCNGGA 1 cut(s) 1476
PinAI ACCGGT 2 cut(s) 228, 830
PkrI GCNGC 4 cut(s) 215, 284, 375, 996
PluTI GGCGCC 1 cut(s) 279
PpuMI RGGWCCY 2 cut(s) 860, 1167
PscI ACATGT 1 cut(s) 744
Psp124BI GAGCTC 2 cut(s) 174, 1152
Psp5II RGGWCCY 2 cut(s) 860, 1167
PspLI CGTACG 1 cut(s) 147
PspN4I GGNNCC 5 cut(s) 90, 277, 800, 829, 1497
PspPI GGNCC 5 cut(s) 88, 860, 1139, 1167, 1220
PspPPI RGGWCCY 2 cut(s) 860, 1167
PsuI RGATCY 2 cut(s) 882, 1435
PvuII CAGCTG 2 cut(s) 373, 788
RsaI GTAC 8 cut(s) 149, 158, 227, 291, 829, 1261, 1407, 1471
RsaNI GTAC 8 cut(s) 148, 157, 226, 290, 828, 1260, 1406, 1470
SacI GAGCTC 2 cut(s) 174, 1152
SaqAI TTAA 3 cut(s) 20, 515, 1431
SatI GCNGC 4 cut(s) 214, 283, 374, 995
Sau3AI GATC 8 cut(s) 143, 339, 497, 882, 892, 1072, 1361, 1435
Sau96I GGNCC 5 cut(s) 88, 860, 1139, 1167, 1220
ScrFI CCNGG 1 cut(s) 1478
SduI GDGCHC 4 cut(s) 74, 174, 1152, 1327
SfaNI GCATC 4 cut(s) 37, 613, 958, 964
SfoI GGCGCC 1 cut(s) 277
SfuI TTCGAA 1 cut(s) 763
SinI GGWCC 4 cut(s) 88, 860, 1139, 1167
SmlI CTYRAG 3 cut(s) 332, 514, 650
SmoI CTYRAG 3 cut(s) 332, 514, 650
SpeI ACTAGT 1 cut(s) 1213
SsiI CCGC 3 cut(s) 200, 506, 523
SspDI GGCGCC 1 cut(s) 275
SspI AATATT 1 cut(s) 346
SspMI CTAG 3 cut(s) 608, 978, 1214
SstI GAGCTC 2 cut(s) 174, 1152
StyD4I CCNGG 1 cut(s) 1476
StyI CCWWGG 4 cut(s) 78, 195, 415, 802
TaaI ACNGT 6 cut(s) 119, 127, 152, 736, 1178, 1403
TaiI ACGT 1 cut(s) 227
TaqI TCGA 3 cut(s) 45, 763, 1454
TatI WGTACW 4 cut(s) 156, 289, 1259, 1405
TfiI GAWTC 5 cut(s) 98, 455, 1031, 1184, 1378
Tru1I TTAA 3 cut(s) 20, 515, 1431
Tru9I TTAA 3 cut(s) 20, 515, 1431
TscAI CASTG 4 cut(s) 124, 277, 1056, 1183
TseFI GTSAC 1 cut(s) 1271
TseI GCWGC 4 cut(s) 213, 282, 373, 994
Tsp45I GTSAC 1 cut(s) 1271
TspDTI ATGAA 6 cut(s) 422, 870, 1003, 1103, 1357, 1477
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 4 cut(s) 124, 277, 1056, 1183
Vha464I CTTAAG 1 cut(s) 514
VpaK11BI GGWCC 4 cut(s) 88, 860, 1139, 1167
XapI RAATTY 4 cut(s) 428, 700, 759, 836
XceI RCATGY 2 cut(s) 748, 1301
XcmI CCANNNNNNNNNTGG 1 cut(s) 792
XmiI GTMKAC 1 cut(s) 1252
XmnI GAANNNNTTC 2 cut(s) 1311, 1382
XspI CTAG 3 cut(s) 608, 978, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.