Rorug02G0563500

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005843
Physical Location & Seq
Forward (+)
68866126 .. 68869906
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0563500.1

Sequence Viewer

Length: 1260 bp
ATGGTTAATAGAGGTGATCTGGTTATCATTGGCATCTCTGCTGGTGTAGCCCTTGGAATTTTGATAGCTGCACTCATATTTTTCGGAATAAGGTGGTACCAAAAGCGTGCCAATCTTCGACGATGTGCTAATGAGCGTAGTTTAACAACTCTTCCTATACGCACAAATGGCTTAGGCACAAGCATCGACCTTAGTGTATCTCTTTCAACTAACATACCTATTCAAGGATCCGAGAAGATTAAAAAAAGTTCTCCACTCTCCTGGTGGAATAATCACAACAAAGACCGATTTCCTTCCGCATCAGGCGTACTTAGATACTCTTACAAGGATGTGCAGAAAGCTACACAGAATTTCACAACAATTTTAGGACAAGGGGCTTTTGGTCCAGTATATAAAGCAACAATGCCTGCTGGAGAGGTAGTAGCTGTGAAGGTGCTTGCTTCAGATTCCAAGCAGGGAGAGAAAGAGTTCCAAACAGAGGTATCTCTGTTAGGAAGACTGCATCATCGAAATCTAGTGAACCTCGTAGGTTACTGTGTAGATAAAGGACAGCGTATGTTAATCTATGAGTTCATGAGTAATGGCAGCTTATCAAACCTTCTGTACAGTGAAGAACAACAGGTTTTGAGCTGGGATGAAAGGCTGCAAATTGCTCTTGATATTTCGCATGGAATAGAGTACCTTCATGAAGGGGCAGCCCCACCAGTCATACATCGTGATTTAAAGTCCGCTAATATATTGTTAGACCAATCAATGAGAGCTAAGGTTGCTGATTTTGGACTGTCTAAGGAAGAGGTGTTTGATGGTCGGAATTCTGGCCTCAAAGGTACCTATGGCTACATAGATCCGGAATACATGTCTACAAACAAGTTCACAATGAAGAGTGACATTTACAGTTTTGGGATCATCATCTTTGAACTCATAACAGCCATCCACCCTCACCAAAACCTAATGGAATATGTTAATCTTGCTTCTATGGCTCCAGATGGTGTTGACGAAATACTTGATAACAAGCTAGTTGGGGAATGCAACCTTGAAGAAGTGAGGAGGCTGGCTAAAATTGCTCACAGATGCTTACTGAAATTACCGAGAAAGCGGCCTTCCATCGGAGAGATTTCACAGGCTATATTGAAGATTAAACAGATGCGTCTTGATAAAAGGGATACCATGTCTTTTGCAGGTGAAGAATTTTCACGGGCAGTAAGCAGAATAGAGGTTCAGCAGGTGGAGCTGGTTAGGCTGGCCAGCACGAAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001101 GO:0001932 GO:0001934 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009753 GO:0009755 GO:0009791 GO:0009814 GO:0009864 GO:0009866 GO:0009867 GO:0009873 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033206 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04016 ko05418 map04016 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

419

Amino Acids

46.89

Weight (kDa)

8.94

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 118 - 375 7.3e-51 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 120 - 374 3.3e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1169, 1213
Acc36I ACCTGC 2 cut(s) 1169, 1213
Acc65I GGTACC 2 cut(s) 96, 827
AccB1I GGYRCC 2 cut(s) 96, 827
AccI GTMKAC 1 cut(s) 860
AccIII TCCGGA 1 cut(s) 847
AciI CCGC 3 cut(s) 297, 729, 1096
AclWI GGATC 4 cut(s) 222, 235, 839, 911
AcoI YGGCCR 1 cut(s) 1242
AcsI RAATTY 4 cut(s) 57, 349, 811, 1187
AcuI CTGAAG 1 cut(s) 426
AfaI GTAC 5 cut(s) 98, 309, 605, 680, 829
AfiI CCNNNNNNNGG 3 cut(s) 224, 478, 1106
AflIII ACRYGT 1 cut(s) 855
AgsI TTSAA 5 cut(s) 207, 224, 917, 1037, 1132
AjnI CCWGG 1 cut(s) 260
AluBI AGCT 8 cut(s) 68, 341, 425, 588, 630, 761, 1015, 1231
AluI AGCT 8 cut(s) 68, 341, 425, 588, 630, 761, 1015, 1231
AlwI GGATC 4 cut(s) 222, 235, 839, 911
Aor13HI TCCGGA 1 cut(s) 847
AoxI GGCC 3 cut(s) 817, 1097, 1242
ApeKI GCWGC 4 cut(s) 68, 585, 643, 695
ApoI RAATTY 4 cut(s) 57, 349, 811, 1187
Asp700I GAANNNNTTC 1 cut(s) 467
Asp718I GGTACC 2 cut(s) 96, 827
AspS9I GGNCC 1 cut(s) 383
AsuHPI GGTGA 3 cut(s) 26, 932, 1193
AvaII GGWCC 1 cut(s) 383
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
BalI TGGCCA 1 cut(s) 1244
BamHI GGATCC 1 cut(s) 227
BanI GGYRCC 2 cut(s) 96, 827
BbsI GAAGAC 1 cut(s) 502
BbvI GCAGC 4 cut(s) 55, 597, 630, 707
BccI CCATC 4 cut(s) 797, 938, 980, 1112
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 262
BciVI GTATCC 1 cut(s) 1156
BfaI CTAG 2 cut(s) 515, 1016
BfuAI ACCTGC 2 cut(s) 1169, 1213
BfuI GTATCC 1 cut(s) 1156
BisI GCNGC 5 cut(s) 69, 586, 644, 696, 1097
BlsI GCNGC 5 cut(s) 70, 587, 645, 697, 1098
Bme1390I CCNGG 1 cut(s) 262
Bme18I GGWCC 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 4 cut(s) 98, 229, 829, 981
BmrFI CCNGG 1 cut(s) 262
BmsI GCATC 6 cut(s) 42, 192, 308, 511, 1061, 1134
BpiI GAAGAC 1 cut(s) 502
BpmI CTGGAG 2 cut(s) 432, 966
Bpu10I CCTNAGC 2 cut(s) 172, 762
BsaBI GATNNNNATC 1 cut(s) 908
BsaJI CCNNGG 1 cut(s) 52
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 478, 1106
Bse1I ACTGG 2 cut(s) 386, 704
Bse8I GATNNNNATC 1 cut(s) 908
BseAI TCCGGA 1 cut(s) 847
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 3 cut(s) 334, 640, 930
BseJI GATNNNNATC 1 cut(s) 908
BseLI CCNNNNNNNGG 3 cut(s) 224, 478, 1106
BseNI ACTGG 2 cut(s) 386, 704
BseRI GAGGAG 1 cut(s) 1060
BseXI GCAGC 4 cut(s) 55, 597, 630, 707
BseYI CCCAGC 1 cut(s) 630
BsgI GTGCAG 2 cut(s) 54, 353
BshFI GGCC 3 cut(s) 819, 1099, 1244
BshNI GGYRCC 2 cut(s) 96, 827
BsiSI CCGG 1 cut(s) 848
BslI CCNNNNNNNGG 3 cut(s) 224, 478, 1106
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 3 cut(s) 819, 1099, 1244
Bsp13I TCCGGA 1 cut(s) 847
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 4 cut(s) 16, 227, 844, 903
BspACI CCGC 3 cut(s) 297, 729, 1096
BspANI GGCC 3 cut(s) 819, 1099, 1244
BspEI TCCGGA 1 cut(s) 847
BspHI TCATGA 2 cut(s) 573, 685
BspLI GGNNCC 4 cut(s) 98, 229, 829, 981
BspMI ACCTGC 2 cut(s) 1169, 1213
BspPI GGATC 4 cut(s) 222, 235, 839, 911
BspT107I GGYRCC 2 cut(s) 96, 827
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 2 cut(s) 386, 704
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 4 cut(s) 16, 227, 844, 903
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 4 cut(s) 536, 608, 783, 896
Bst6I CTCTTC 3 cut(s) 156, 786, 875
BstAUI TGTACA 1 cut(s) 603
BstC8I GCNNGC 6 cut(s) 108, 408, 438, 1053, 1242, 1246
BstDEI CTNAG 5 cut(s) 172, 191, 311, 762, 786
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 334, 640, 930
BstKTI GATC 4 cut(s) 19, 230, 847, 906
BstMBI GATC 4 cut(s) 16, 227, 844, 903
BstMWI GCNNNNNNNGC 7 cut(s) 47, 168, 767, 977, 1061, 1228, 1237
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 1 cut(s) 260
BstV1I GCAGC 4 cut(s) 55, 597, 630, 707
BstV2I GAAGAC 1 cut(s) 502
BstX2I RGATCY 2 cut(s) 227, 844
BstXI CCANNNNNNTGG 1 cut(s) 261
BstYI RGATCY 2 cut(s) 227, 844
BsuI GTATCC 1 cut(s) 1156
BsuRI GGCC 3 cut(s) 819, 1099, 1244
BtsCI GGATG 3 cut(s) 334, 640, 930
BtsIMutI CAGTG 1 cut(s) 613
BveI ACCTGC 2 cut(s) 1169, 1213
Cac8I GCNNGC 6 cut(s) 108, 408, 438, 1053, 1242, 1246
CciI TCATGA 2 cut(s) 573, 685
Cfr13I GGNCC 1 cut(s) 383
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 5 cut(s) 97, 308, 604, 679, 828
CviAII CATG 5 cut(s) 574, 668, 686, 856, 1168
CviQI GTAC 5 cut(s) 97, 308, 604, 679, 828
DdeI CTNAG 5 cut(s) 172, 191, 311, 762, 786
DpnI GATC 4 cut(s) 18, 229, 846, 905
DpnII GATC 4 cut(s) 16, 227, 844, 903
DraI TTTAAA 1 cut(s) 723
EaeI YGGCCR 1 cut(s) 1242
Eam1104I CTCTTC 3 cut(s) 156, 786, 875
EarI CTCTTC 3 cut(s) 156, 786, 875
Eco130I CCWWGG 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoRI GAATTC 1 cut(s) 811
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 5 cut(s) 577, 671, 689, 859, 1171
FatI CATG 5 cut(s) 573, 667, 685, 855, 1167
FblI GTMKAC 1 cut(s) 860
Fnu4HI GCNGC 5 cut(s) 69, 586, 644, 696, 1097
FokI GGATG 3 cut(s) 341, 647, 917
Fsp4HI GCNGC 5 cut(s) 69, 586, 644, 696, 1097
FspBI CTAG 2 cut(s) 515, 1016
GluI GCNGC 5 cut(s) 69, 586, 644, 696, 1097
GsaI CCCAGC 1 cut(s) 634
GsuI CTGGAG 2 cut(s) 432, 966
HaeIII GGCC 3 cut(s) 819, 1099, 1244
HapII CCGG 1 cut(s) 848
HgaI GACGC 1 cut(s) 1136
Hin1II CATG 5 cut(s) 577, 671, 689, 859, 1171
HincII GTYRAC 1 cut(s) 994
HindII GTYRAC 1 cut(s) 994
HinfI GANTC 1 cut(s) 446
HpaII CCGG 1 cut(s) 848
HphI GGTGA 3 cut(s) 26, 932, 1193
Hpy166II GTNNAC 4 cut(s) 520, 861, 873, 994
Hpy188I TCNGA 5 cut(s) 86, 232, 445, 810, 1109
Hpy188III TCNNGA 7 cut(s) 574, 656, 686, 716, 848, 983, 1151
Hpy8I GTNNAC 4 cut(s) 520, 861, 873, 994
Hpy99I CGWCG 1 cut(s) 123
HpyAV CCTTC 6 cut(s) 303, 424, 608, 683, 692, 1110
HpyCH4III ACNGT 4 cut(s) 536, 608, 783, 896
HpyCH4V TGCA 6 cut(s) 71, 334, 502, 646, 1029, 1178
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 168, 767, 977, 1061, 1228, 1237
HpyF3I CTNAG 5 cut(s) 172, 191, 311, 762, 786
Hsp92II CATG 5 cut(s) 577, 671, 689, 859, 1171
Kpn2I TCCGGA 1 cut(s) 847
KpnI GGTACC 2 cut(s) 100, 831
Kzo9I GATC 4 cut(s) 16, 227, 844, 903
LmnI GCTCC 2 cut(s) 985, 1228
Lsp1109I GCAGC 4 cut(s) 55, 597, 630, 707
LweI GCATC 6 cut(s) 42, 192, 308, 511, 1061, 1134
MaeI CTAG 2 cut(s) 515, 1016
MaeIII GTNAC 2 cut(s) 530, 884
MalI GATC 4 cut(s) 18, 229, 846, 905
MboI GATC 4 cut(s) 16, 227, 844, 903
MflI RGATCY 2 cut(s) 227, 844
MlsI TGGCCA 1 cut(s) 1244
MluCI AATT 8 cut(s) 57, 349, 360, 648, 811, 1059, 1082, 1187
MluNI TGGCCA 1 cut(s) 1244
MmeI TCCRAC 1 cut(s) 788
Mox20I TGGCCA 1 cut(s) 1244
MroI TCCGGA 1 cut(s) 847
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 1244
MseI TTAA 7 cut(s) 6, 143, 240, 560, 722, 963, 1137
Msp20I TGGCCA 1 cut(s) 1244
MspI CCGG 1 cut(s) 848
MspR9I CCNGG 1 cut(s) 262
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 7 cut(s) 47, 168, 767, 977, 1061, 1228, 1237
NdeII GATC 4 cut(s) 16, 227, 844, 903
NlaIII CATG 5 cut(s) 577, 671, 689, 859, 1171
NlaIV GGNNCC 4 cut(s) 98, 229, 829, 981
NmuCI GTSAC 1 cut(s) 884
NspI RCATGY 1 cut(s) 859
PagI TCATGA 2 cut(s) 573, 685
PaqCI CACCTGC 2 cut(s) 1169, 1213
PciI ACATGT 1 cut(s) 855
PctI GAATGC 1 cut(s) 1031
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 446
PkrI GCNGC 5 cut(s) 70, 587, 645, 697, 1098
PscI ACATGT 1 cut(s) 855
Psp6I CCWGG 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 630
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 4 cut(s) 98, 229, 829, 981
PspPI GGNCC 1 cut(s) 383
PsuI RGATCY 2 cut(s) 227, 844
RsaI GTAC 5 cut(s) 98, 309, 605, 680, 829
RsaNI GTAC 5 cut(s) 97, 308, 604, 679, 828
SaqAI TTAA 7 cut(s) 6, 143, 240, 560, 722, 963, 1137
SatI GCNGC 5 cut(s) 69, 586, 644, 696, 1097
Sau3AI GATC 4 cut(s) 16, 227, 844, 903
Sau96I GGNCC 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 262
SfaNI GCATC 6 cut(s) 42, 192, 308, 511, 1061, 1134
SinI GGWCC 1 cut(s) 383
Sse9I AATT 8 cut(s) 57, 349, 360, 648, 811, 1059, 1082, 1187
SsiI CCGC 3 cut(s) 297, 729, 1096
SspMI CTAG 2 cut(s) 515, 1016
StyD4I CCNGG 1 cut(s) 260
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 4 cut(s) 536, 608, 783, 896
TaqI TCGA 3 cut(s) 118, 186, 508
TaqII GACCGA 1 cut(s) 300
TasI AATT 8 cut(s) 57, 349, 360, 648, 811, 1059, 1082, 1187
TatI WGTACW 1 cut(s) 603
TauI GCSGC 1 cut(s) 1099
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 7 cut(s) 6, 143, 240, 560, 722, 963, 1137
Tru9I TTAA 7 cut(s) 6, 143, 240, 560, 722, 963, 1137
TscAI CASTG 1 cut(s) 613
TseFI GTSAC 1 cut(s) 884
TseI GCWGC 4 cut(s) 68, 585, 643, 695
Tsp45I GTSAC 1 cut(s) 884
TspDTI ATGAA 5 cut(s) 562, 651, 674, 702, 893
TspRI CASTG 1 cut(s) 613
VpaK11BI GGWCC 1 cut(s) 383
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 4 cut(s) 57, 349, 811, 1187
XceI RCATGY 1 cut(s) 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 860
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 2 cut(s) 515, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.