MD09G1027900.v1.1

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005843
Physical Location & Seq
Reverse (-)
1730527 .. 1733711
3185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1027900.v1.1.491

Sequence Viewer

Length: 1560 bp
ATGGCTGGATTAGAAGAACTTAGAAAAAAGCTCACTCCTTTGTTCGACGCCGAAAAGGGCTTCTCCGCCGGAGGCCCTTCCTTGGAGCTCGACCCTTGCGATTCCTACACTCTATCGGACAGTGGAACGGTGAACTTGCTGAGCAGATCGTACGGCGTGTACAACATCAACGAACTCGGATTGCAGAAATGCACGACCTTGGCGGTGGACGACAGCGCCGAGAAGACCTACCGGTGCGCCTCGCGGGAGATGAGGGTGTTTGGGGCAATCGGCAGCGGCGCCAGCAGTGTTGTGCAGAGAGCTATCCACATTCCCACTCATCGGATTCTAGCTCTCAAGAAGATCAACATTTTCGAGAAGGAGAAAAGGCAACAGCTGCTTACTGAGATACGAACACTATGCGAAGCACCTTGTTACCAAGGTCTTGTAGAATTTCACGGCGCATTTTATACGCCGGATTCTGGACAAATAAGCATAGCTTTGGAGTATATGGATGGAGGATCGTTGGCGGATGTCCTAAGATTGCAGAAAAGAATACCTGAACCCCTACTTTCTTCTATGTTTCAAAAGCTTCTACATGGACTGAGCTACTTGCACGGAGTTAGGCATCTAGTCCACAGAGACATAAAGCCCGCCAATTTGCTTGTGAATCTCAAGGGTGAGCCAAAAATAACAGATTTTGGTATAAGTGCTGGCTTAGAGAATTCAATGGCAATGTGTGCGACATTTGTTGGGACGGTCACATACATGTCACCTGAGCGAATTCGAAATGAGAATTATTCTTATCCAGCTGATATTTGGAGCCTTGGTCTTGCTCTCTTTGAGTGTGGTACTGGAGAATTCCCGTATACAGCTACTGAAGGACCTGTCAATCTTATGCTGCAGATCTTGGATGATCCATCACCTACACCTTCAAAACACAAATTTTCACCAGAGTTTTGCTCGTTTATTGAAGCCTGCTTGCAAAAGGATCCAGATGCTAGGCCAACAGCAGAGCAGCTACTTTCACACCCCTTCATCACAAAGTACGAGGATTCCCAAGTTGACTTAGCAGCATTTGTCCGAAGCGTATTTGATCCAACACAAAGGATGAAGGACTTGGCAGATATGCTTACGATACATTTCTACCTACTCTTTGATGGACCTGATGAGCAATGGCAACATACAAGGACGTTATATAATGAAGATTCAGTATTCAGTTTCTCAGGAAAGCAACATGTCGGCCCAAATGATATCTTTGCAACTCTATCAAGTATCCGGAGTACACTAGCTGGTGACTGGCCTCCAGAAAGACTTGTGCACGTTGTAGAAAAGCTTCAGTGTCGCGCTCACGGTCAGGATGGAGTAGCGATTAGGGTGTCGGGATCATTCATCGTTGGGAATCAGTTCCTTATCTGTGGAGATGGCGTACAAGTGGAGGGCTTGCCAAGCATCAAAGATCTTCCCATTGATATTTCGAGTAAGCGAATGGGTACGTTTCAGGAGCAGTTCATCATGGAGCCGAGCAACTTAATTGGTTGCTACTCCATAGCTGAACAAGAACTTTACATTAAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001101 GO:0001932 GO:0001934 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009753 GO:0009755 GO:0009791 GO:0009814 GO:0009864 GO:0009866 GO:0009867 GO:0009873 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033206 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04016 ko05418 map04016 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

520

Amino Acids

57.73

Weight (kDa)

5.39

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 87 - 340 6.9e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 89 - 336 2e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 848
AccII CGCG 2 cut(s) 244, 1326
AccIII TCCGGA 1 cut(s) 1257
AciI CCGC 6 cut(s) 66, 203, 244, 276, 509, 633
AclWI GGATC 6 cut(s) 508, 890, 965, 978, 1070, 1372
AcsI RAATTY 5 cut(s) 431, 703, 762, 839, 923
AcuI CTGAAG 2 cut(s) 879, 1301
AcyI GRCGYC 2 cut(s) 48, 279
AfaI GTAC 7 cut(s) 152, 161, 832, 1028, 1264, 1410, 1474
AfiI CCNNNNNNNGG 3 cut(s) 82, 321, 461
AflIII ACRYGT 2 cut(s) 747, 1216
AgeI ACCGGT 1 cut(s) 231
AgsI TTSAA 4 cut(s) 566, 708, 915, 953
Alw21I GWGCWC 2 cut(s) 90, 1302
Alw26I GTCTC 1 cut(s) 615
Alw44I GTGCAC 1 cut(s) 1298
AlwI GGATC 6 cut(s) 508, 890, 965, 978, 1070, 1372
AlwNI CAGNNNCTG 1 cut(s) 857
Aor13HI TCCGGA 1 cut(s) 1257
AoxI GGCC 4 cut(s) 73, 983, 1222, 1280
ApaLI GTGCAC 1 cut(s) 1298
ApeKI GCWGC 5 cut(s) 273, 376, 880, 997, 1052
ApoI RAATTY 5 cut(s) 431, 703, 762, 839, 923
AsiGI ACCGGT 1 cut(s) 231
Asp700I GAANNNNTTC 2 cut(s) 1314, 1385
AspLEI GCGC 5 cut(s) 218, 239, 281, 443, 1328
AspS9I GGNCC 4 cut(s) 74, 863, 1142, 1223
AsuHPI GGTGA 6 cut(s) 142, 671, 744, 894, 921, 1286
AsuII TTCGAA 1 cut(s) 766
AvaII GGWCC 2 cut(s) 863, 1142
BaeGI GKGCMC 1 cut(s) 1302
BamHI GGATCC 1 cut(s) 970
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 90
BarI GAAGNNNNNNTAC 2 cut(s) 1299, 1331
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 2 cut(s) 90, 1302
BbvI GCAGC 5 cut(s) 285, 363, 867, 1009, 1064
BccI CCATC 5 cut(s) 488, 907, 1133, 1334, 1397
BceAI ACGGC 2 cut(s) 169, 454
BcgI CGANNNNNNTGC 2 cut(s) 381, 415
BciVI GTATCC 1 cut(s) 1265
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 4 cut(s) 329, 611, 981, 1268
BfmI CTRYAG 1 cut(s) 881
BfoI RGCGCY 2 cut(s) 219, 282
BfuI GTATCC 1 cut(s) 1265
BglII AGATCT 2 cut(s) 885, 1438
BisI GCNGC 6 cut(s) 274, 277, 377, 881, 998, 1053
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 6 cut(s) 275, 278, 378, 882, 999, 1054
Bme18I GGWCC 2 cut(s) 863, 1142
BmgT120I GGNCC 4 cut(s) 74, 863, 1142, 1223
BmiI GGNNCC 4 cut(s) 280, 803, 972, 1500
BmsI GCATC 3 cut(s) 616, 967, 1440
BpiI GAAGAC 1 cut(s) 230
BplI GAGNNNNNCTC 2 cut(s) 926, 958
BpmI CTGGAG 2 cut(s) 855, 1269
Bpu10I CCTNAGC 1 cut(s) 756
Bpu1102I GCTNAGC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 766
BpuEI CTTGAG 2 cut(s) 320, 638
BsaHI GRCGYC 2 cut(s) 48, 279
BsaJI CCNNGG 4 cut(s) 81, 198, 418, 805
BsaWI WCCGGW 2 cut(s) 231, 1257
BsaXI ACNNNNNCTCC 4 cut(s) 793, 823, 1392, 1422
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 321, 461
Bse118I RCCGGY 1 cut(s) 231
Bse1I ACTGG 2 cut(s) 838, 1283
Bse3DI GCAATG 2 cut(s) 720, 1160
BseAI TCCGGA 1 cut(s) 1257
BseDI CCNNGG 4 cut(s) 81, 198, 418, 805
BseGI GGATG 5 cut(s) 499, 517, 898, 1095, 1345
BseLI CCNNNNNNNGG 3 cut(s) 82, 321, 461
BseMI GCAATG 2 cut(s) 720, 1160
BseMII CTCAG 5 cut(s) 131, 375, 575, 747, 1218
BseNI ACTGG 2 cut(s) 838, 1283
BseSI GKGCMC 1 cut(s) 1302
BseXI GCAGC 5 cut(s) 285, 363, 867, 1009, 1064
BsgI GTGCAG 1 cut(s) 314
Bsh1236I CGCG 2 cut(s) 244, 1326
BshFI GGCC 4 cut(s) 75, 985, 1224, 1282
BshNI GGYRCC 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 231
BsiHKAI GWGCWC 2 cut(s) 90, 1302
BsiSI CCGG 4 cut(s) 69, 232, 455, 1258
BsiWI CGTACG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 748
BslI CCNNNNNNNGG 3 cut(s) 82, 321, 461
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 748
BsnI GGCC 4 cut(s) 75, 985, 1224, 1282
Bsp119I TTCGAA 1 cut(s) 766
Bsp1286I GDGCHC 2 cut(s) 90, 1302
Bsp13I TCCGGA 1 cut(s) 1257
Bsp1407I TGTACA 1 cut(s) 159
Bsp143I GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 6 cut(s) 66, 203, 244, 276, 509, 633
BspANI GGCC 4 cut(s) 75, 985, 1224, 1282
BspCNI CTCAG 5 cut(s) 132, 376, 576, 748, 1217
BspEI TCCGGA 1 cut(s) 1257
BspFNI CGCG 2 cut(s) 244, 1326
BspLI GGNNCC 4 cut(s) 280, 803, 972, 1500
BspMAI CTGCAG 1 cut(s) 885
BspPI GGATC 6 cut(s) 508, 890, 965, 978, 1070, 1372
BspT104I TTCGAA 1 cut(s) 766
BspT107I GGYRCC 1 cut(s) 278
BsrDI GCAATG 2 cut(s) 720, 1160
BsrFI RCCGGY 1 cut(s) 231
BsrGI TGTACA 1 cut(s) 159
BsrI ACTGG 2 cut(s) 838, 1283
BssAI RCCGGY 1 cut(s) 231
BssECI CCNNGG 4 cut(s) 81, 198, 418, 805
BssMI GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
BssNAI GTATAC 1 cut(s) 849
BssNI GRCGYC 2 cut(s) 48, 279
BssT1I CCWWGG 4 cut(s) 81, 198, 418, 805
Bst1107I GTATAC 1 cut(s) 849
Bst4CI ACNGT 4 cut(s) 122, 130, 739, 1334
BstACI GRCGYC 2 cut(s) 48, 279
BstAPI GCANNNNNTGC 2 cut(s) 376, 719
BstAUI TGTACA 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 766
BstC8I GCNNGC 6 cut(s) 283, 633, 694, 958, 962, 1424
BstDEI CTNAG 9 cut(s) 20, 140, 384, 518, 584, 697, 756, 1048, 1204
BstF5I GGATG 5 cut(s) 499, 517, 898, 1095, 1345
BstFNI CGCG 2 cut(s) 244, 1326
BstH2I RGCGCY 2 cut(s) 219, 282
BstHHI GCGC 5 cut(s) 218, 239, 281, 443, 1328
BstKTI GATC 9 cut(s) 149, 345, 503, 888, 898, 973, 1078, 1367, 1441
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
BstMWI GCNNNNNNNGC 4 cut(s) 282, 376, 719, 1428
BstNSI RCATGY 2 cut(s) 751, 1220
BstSFI CTRYAG 1 cut(s) 881
BstSLI GKGCMC 1 cut(s) 1302
BstUI CGCG 2 cut(s) 244, 1326
BstV1I GCAGC 5 cut(s) 285, 363, 867, 1009, 1064
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 3 cut(s) 885, 970, 1438
BstYI RGATCY 3 cut(s) 885, 970, 1438
BstZ17I GTATAC 1 cut(s) 849
BsuI GTATCC 1 cut(s) 1265
BsuRI GGCC 4 cut(s) 75, 985, 1224, 1282
BtsCI GGATG 5 cut(s) 499, 517, 898, 1095, 1345
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 3 cut(s) 127, 292, 1325
Cac8I GCNNGC 6 cut(s) 283, 633, 694, 958, 962, 1424
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 5 cut(s) 218, 239, 281, 443, 1328
Cfr10I RCCGGY 1 cut(s) 231
Cfr13I GGNCC 4 cut(s) 74, 863, 1142, 1223
CseI GACGC 1 cut(s) 56
Csp6I GTAC 7 cut(s) 151, 160, 831, 1027, 1263, 1409, 1473
CspAI ACCGGT 1 cut(s) 231
CviAII CATG 4 cut(s) 578, 748, 1217, 1495
CviQI GTAC 7 cut(s) 151, 160, 831, 1027, 1263, 1409, 1473
DdeI CTNAG 9 cut(s) 20, 140, 384, 518, 584, 697, 756, 1048, 1204
DinI GGCGCC 1 cut(s) 280
DpnI GATC 9 cut(s) 148, 344, 502, 887, 897, 972, 1077, 1366, 1440
DpnII GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
EciI GGCGGA 2 cut(s) 55, 524
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 4 cut(s) 81, 198, 418, 805
Eco24I GRGCYC 1 cut(s) 90
Eco32I GATATC 1 cut(s) 1234
Eco47I GGWCC 2 cut(s) 863, 1142
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 879, 1301
EcoICRI GAGCTC 1 cut(s) 88
EcoO109I RGGNCCY 2 cut(s) 74, 863
EcoRI GAATTC 3 cut(s) 703, 762, 839
EcoRV GATATC 1 cut(s) 1234
EcoT14I CCWWGG 4 cut(s) 81, 198, 418, 805
EcoT38I GRGCYC 1 cut(s) 90
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 4 cut(s) 81, 198, 418, 805
FaeI CATG 4 cut(s) 581, 751, 1220, 1498
FalI AAGNNNNNCTT 2 cut(s) 463, 495
FaqI GGGAC 1 cut(s) 748
FatI CATG 4 cut(s) 577, 747, 1216, 1494
FauI CCCGC 2 cut(s) 237, 640
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 6 cut(s) 274, 277, 377, 881, 998, 1053
FokI GGATG 5 cut(s) 506, 524, 905, 1102, 1352
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 6 cut(s) 274, 277, 377, 881, 998, 1053
FspBI CTAG 4 cut(s) 329, 611, 981, 1268
GlaI GCGC 5 cut(s) 217, 238, 280, 442, 1327
GluI GCNGC 6 cut(s) 274, 277, 377, 881, 998, 1053
GsuI CTGGAG 2 cut(s) 855, 1269
HaeII RGCGCY 2 cut(s) 219, 282
HaeIII GGCC 4 cut(s) 75, 985, 1224, 1282
HapII CCGG 4 cut(s) 69, 232, 455, 1258
HgaI GACGC 1 cut(s) 56
HhaI GCGC 5 cut(s) 218, 239, 281, 443, 1328
Hin1I GRCGYC 2 cut(s) 48, 279
Hin1II CATG 4 cut(s) 581, 751, 1220, 1498
Hin6I GCGC 5 cut(s) 216, 237, 279, 441, 1326
HinP1I GCGC 5 cut(s) 216, 237, 279, 441, 1326
HincII GTYRAC 1 cut(s) 1045
HindII GTYRAC 1 cut(s) 1045
HindIII AAGCTT 2 cut(s) 569, 1313
HinfI GANTC 7 cut(s) 101, 325, 458, 649, 1034, 1187, 1381
HpaII CCGG 4 cut(s) 69, 232, 455, 1258
HphI GGTGA 6 cut(s) 142, 671, 744, 894, 921, 1286
Hpy166II GTNNAC 8 cut(s) 133, 160, 208, 616, 849, 1045, 1265, 1300
Hpy188I TCNGA 4 cut(s) 118, 179, 324, 1064
Hpy8I GTNNAC 8 cut(s) 133, 160, 208, 616, 849, 1045, 1265, 1300
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 6 cut(s) 87, 352, 854, 921, 1024, 1087
HpyCH4III ACNGT 4 cut(s) 122, 130, 739, 1334
HpyCH4IV ACGT 3 cut(s) 1172, 1302, 1475
HpyCH4V TGCA 9 cut(s) 184, 192, 295, 526, 595, 883, 964, 1241, 1300
HpyF10VI GCNNNNNNNGC 4 cut(s) 282, 376, 719, 1428
HpyF3I CTNAG 9 cut(s) 20, 140, 384, 518, 584, 697, 756, 1048, 1204
HpySE526I ACGT 3 cut(s) 1172, 1302, 1475
Hsp92I GRCGYC 2 cut(s) 48, 279
Hsp92II CATG 4 cut(s) 581, 751, 1220, 1498
HspAI GCGC 5 cut(s) 216, 237, 279, 441, 1326
KasI GGCGCC 1 cut(s) 278
Kpn2I TCCGGA 1 cut(s) 1257
Kzo9I GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
LmnI GCTCC 4 cut(s) 85, 801, 1483, 1498
Lsp1109I GCAGC 5 cut(s) 285, 363, 867, 1009, 1064
LweI GCATC 3 cut(s) 616, 967, 1440
MaeI CTAG 4 cut(s) 329, 611, 981, 1268
MaeII ACGT 3 cut(s) 1172, 1302, 1475
MaeIII GTNAC 4 cut(s) 413, 739, 750, 1274
MalI GATC 9 cut(s) 148, 344, 502, 887, 897, 972, 1077, 1366, 1440
MboI GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
MboII GAAGA 6 cut(s) 26, 235, 352, 546, 1196, 1433
MflI RGATCY 3 cut(s) 885, 970, 1438
MhlI GDGCHC 2 cut(s) 90, 1302
MluCI AATT 8 cut(s) 431, 637, 703, 762, 775, 839, 923, 1512
Mly113I GGCGCC 1 cut(s) 279
MmeI TCCRAC 1 cut(s) 1103
MnlI CCTC 7 cut(s) 65, 246, 250, 491, 1024, 1293, 1411
MroI TCCGGA 1 cut(s) 1257
MroXI GAANNNNTTC 2 cut(s) 1314, 1385
MseI TTAA 2 cut(s) 1511, 1551
MslI CAYNNNNRTG 1 cut(s) 746
MspA1I CMGCKG 3 cut(s) 276, 376, 791
MspI CCGG 4 cut(s) 69, 232, 455, 1258
MvnI CGCG 2 cut(s) 244, 1326
MwoI GCNNNNNNNGC 4 cut(s) 282, 376, 719, 1428
NarI GGCGCC 1 cut(s) 279
NdeII GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
NlaIII CATG 4 cut(s) 581, 751, 1220, 1498
NlaIV GGNNCC 4 cut(s) 280, 803, 972, 1500
NmeAIII GCCGAG 2 cut(s) 244, 1527
NmuCI GTSAC 3 cut(s) 739, 750, 1274
NspI RCATGY 2 cut(s) 751, 1220
NspV TTCGAA 1 cut(s) 766
PciI ACATGT 2 cut(s) 747, 1216
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 216
PdmI GAANNNNTTC 2 cut(s) 1314, 1385
PfeI GAWTC 7 cut(s) 101, 325, 458, 649, 1034, 1187, 1381
Pfl23II CGTACG 1 cut(s) 150
PinAI ACCGGT 1 cut(s) 231
PkrI GCNGC 6 cut(s) 275, 278, 378, 882, 999, 1054
PluTI GGCGCC 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 863
PscI ACATGT 2 cut(s) 747, 1216
Psp124BI GAGCTC 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 863
PspLI CGTACG 1 cut(s) 150
PspN4I GGNNCC 4 cut(s) 280, 803, 972, 1500
PspPI GGNCC 4 cut(s) 74, 863, 1142, 1223
PspPPI RGGWCCY 1 cut(s) 863
PstI CTGCAG 1 cut(s) 885
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 3 cut(s) 885, 970, 1438
PvuII CAGCTG 2 cut(s) 376, 791
RsaI GTAC 7 cut(s) 152, 161, 832, 1028, 1264, 1410, 1474
RsaNI GTAC 7 cut(s) 151, 160, 831, 1027, 1263, 1409, 1473
RseI CAYNNNNRTG 1 cut(s) 746
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 2 cut(s) 1511, 1551
SatI GCNGC 6 cut(s) 274, 277, 377, 881, 998, 1053
Sau3AI GATC 9 cut(s) 146, 342, 500, 885, 895, 970, 1075, 1364, 1438
Sau96I GGNCC 4 cut(s) 74, 863, 1142, 1223
SduI GDGCHC 2 cut(s) 90, 1302
SfaNI GCATC 3 cut(s) 616, 967, 1440
SfcI CTRYAG 1 cut(s) 881
SfoI GGCGCC 1 cut(s) 280
SfuI TTCGAA 1 cut(s) 766
SinI GGWCC 2 cut(s) 863, 1142
SmiMI CAYNNNNRTG 1 cut(s) 746
SmlI CTYRAG 2 cut(s) 335, 653
SmoI CTYRAG 2 cut(s) 335, 653
Sse9I AATT 8 cut(s) 431, 637, 703, 762, 775, 839, 923, 1512
SsiI CCGC 6 cut(s) 66, 203, 244, 276, 509, 633
SspDI GGCGCC 1 cut(s) 278
SspMI CTAG 4 cut(s) 329, 611, 981, 1268
SstI GAGCTC 1 cut(s) 90
StyI CCWWGG 4 cut(s) 81, 198, 418, 805
TaaI ACNGT 4 cut(s) 122, 130, 739, 1334
TaiI ACGT 3 cut(s) 1175, 1305, 1478
TaqI TCGA 5 cut(s) 45, 90, 354, 766, 1457
TasI AATT 8 cut(s) 431, 637, 703, 762, 775, 839, 923, 1512
TatI WGTACW 2 cut(s) 159, 1262
TauI GCSGC 1 cut(s) 279
TfiI GAWTC 7 cut(s) 101, 325, 458, 649, 1034, 1187, 1381
Tru1I TTAA 2 cut(s) 1511, 1551
Tru9I TTAA 2 cut(s) 1511, 1551
TscAI CASTG 3 cut(s) 127, 292, 1325
TseFI GTSAC 3 cut(s) 739, 750, 1274
TseI GCWGC 5 cut(s) 273, 376, 880, 997, 1052
Tsp45I GTSAC 3 cut(s) 739, 750, 1274
TspDTI ATGAA 5 cut(s) 1006, 1106, 1197, 1360, 1480
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 3 cut(s) 127, 292, 1325
VneI GTGCAC 1 cut(s) 1298
VpaK11BI GGWCC 2 cut(s) 863, 1142
XapI RAATTY 5 cut(s) 431, 703, 762, 839, 923
XceI RCATGY 2 cut(s) 751, 1220
XcmI CCANNNNNNNNNTGG 1 cut(s) 795
XmiI GTMKAC 1 cut(s) 848
XmnI GAANNNNTTC 2 cut(s) 1314, 1385
XspI CTAG 4 cut(s) 329, 611, 981, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.