RchiOBHm_Chr2g0172911

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005843
Physical Location & Seq
Reverse (-)
86113765 .. 86118162
4398 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54017

Sequence Viewer

Length: 1551 bp
ATGGCCGGACTAGAAGAACTGAGGAAAAAGCTGACACCTTTGTTCGATGCAGAGAAGGGTCTGGGCTTGGGATCCTCCACCTTGGACCCGTCAGATTCATACACACTTTCGGACAGAACGGTGGAGTTGCTGAGCCGATCGTACGGCGTGTACAACATCAACGAGCTGGGACTGCAGAAGTGCACGTCTTCTACGGTGGACGACACCACAGAGAAGACTTACCGGTGCGCCTCGCATGAGATGAGGGTGTTTGGGGCCATTGGGAGTGGCGCCAGCAGTGTTGTGCAGAGAGCTATTCACATTCCCACTCATCGGATTATAGCGTTAAAAAAGATCAACATTTTCGAAAAGGAAAAAAGGCAGCAGCTGCTTACTGAGATAAGGACACTATGTGAAGCACCATGTTACCAAGGTCTTGTAGAGTTTCATGGTGCATTTTATACTCCAGATTCCGGACAGATAAGCATAGCTTTGGAGTATATGGATGGAGGGTCACTGGCAGATATCTTACGGTTGCGGAAGAGAATACCCGAACCTCTACTTTCTTCTATGTTTCAGAAGCTTCTACATGGACTGAGCTACTTGCATGGAGTTAGGTATCTAGTACACAGAGACATAAAGCCTGCCAATTTGCTTATAAATCTCAAGGGTGAGCCAAAGATAACAGACTTCGGTATAAGTGCTGGCTTGGAGAATTCTATGGCAATGTGTGCAACATTTGTTGGGACAGTTACATACATGTCGCCTGAGCGAATTCGAAATGAGAATTATTCTTACCCAGCTGATATTTGGAGCCTTGGGCTTGCTCTCTTTGAGTGTGGTACTGGAGAATTTCCATATACAGCTAATGATGGACCTGTCAATCTTATGTTGCAGATCTTAGATGATCCATCACCTACACCTTCAAAACAGAACTTTTCACCAGAGTTCTGCTCGTTTATTGAAGCTTGCTTGCAGAAGGATGCAGATGCTAGACCAACTGCAGAGCAGCTACTTTCACACCCCTTCATTACAAAGTATGAGAAGTCCCAAGTGGACTTAGCAGCATTTGTTCGAAGTGTTTTTGATCCAACGCAAAGGATGAAGGACTTGGCAGATATGCTTACCATACATTTTTACTTGCTCTTTGATGGATCTGATGAGCTCTGGCAACATGCAAAGACGTTATACAATGAAGATTCAGTTTTCAGTTTCTCTGGCAAGCAACTAGTCGGTGCGAATGATATATTTGCCTCACTCTCTAGTATACGAAGTACATTAGCTGGTGACTGGCCTCCTGAGAGGCTTGTGCATGTTGTGGAAAAGCTTCAGTGCCGTGCTCAAGGTCAGGATGGAGTAGCAATTAGGGTATCAGGATCCTTCATTGTTGGGAATCAGTTCCTTATATGTGGTGACGGTGTTCAGGTAGAGGGCTTGGCCAGTATTAAAGATCTTTCCATTGATATTTCGAGTAAGCGAATGGGTACATTCCGCGAGCAGTTCATTATGGAGCCAAGCACTATTATAGGGCGCTACTCAATTGCTAGCCAAGAACTTTATATTATGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001101 GO:0001932 GO:0001934 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009753 GO:0009755 GO:0009791 GO:0009814 GO:0009864 GO:0009866 GO:0009867 GO:0009873 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033206 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04016 ko05418 map04016 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

516

Amino Acids

57.41

Weight (kDa)

5.49

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 84 - 337 1.4e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 86 - 333 6.7e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AccB1I GGYRCC 1 cut(s) 269
AccI GTMKAC 1 cut(s) 1246
AccII CGCG 1 cut(s) 1473
AccIII TCCGGA 1 cut(s) 452
AciI CCGC 2 cut(s) 517, 1471
AclWI GGATC 7 cut(s) 66, 79, 881, 1061, 1141, 1350, 1363
AcoI YGGCCR 2 cut(s) 3, 1416
AcsI RAATTY 3 cut(s) 694, 753, 830
AcuI CTGAAG 1 cut(s) 1292
AcyI GRCGYC 1 cut(s) 270
AdeI CACNNNGTG 1 cut(s) 392
AfaI GTAC 6 cut(s) 143, 152, 606, 823, 1255, 1465
AfiI CCNNNNNNNGG 2 cut(s) 312, 452
AflIII ACRYGT 1 cut(s) 738
AgeI ACCGGT 1 cut(s) 222
AgsI TTSAA 2 cut(s) 906, 944
AhlI ACTAGT 1 cut(s) 1207
AjiI CACGTC 1 cut(s) 186
Alw21I GWGCWC 3 cut(s) 185, 1146, 1321
Alw26I GTCTC 1 cut(s) 606
Alw44I GTGCAC 1 cut(s) 181
AlwI GGATC 7 cut(s) 66, 79, 881, 1061, 1141, 1350, 1363
AlwNI CAGNNNCTG 1 cut(s) 367
Aor13HI TCCGGA 1 cut(s) 452
AoxI GGCC 4 cut(s) 3, 255, 1271, 1416
ApaLI GTGCAC 1 cut(s) 181
ApeKI GCWGC 5 cut(s) 361, 364, 367, 988, 1043
ApoI RAATTY 3 cut(s) 694, 753, 830
AsiGI ACCGGT 1 cut(s) 222
Asp700I GAANNNNTTC 2 cut(s) 1305, 1376
AspLEI GCGC 3 cut(s) 230, 272, 1512
AspS9I GGNCC 3 cut(s) 85, 255, 854
AsuHPI GGTGA 5 cut(s) 662, 885, 912, 1277, 1403
AsuII TTCGAA 3 cut(s) 345, 757, 1054
AsuNHI GCTAGC 1 cut(s) 1523
AvaII GGWCC 2 cut(s) 85, 854
BaeGI GKGCMC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 1418
BamHI GGATCC 2 cut(s) 71, 1355
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 1146
BbsI GAAGAC 2 cut(s) 180, 221
Bbv12I GWGCWC 3 cut(s) 185, 1146, 1321
BbvI GCAGC 5 cut(s) 354, 373, 376, 1000, 1055
BccI CCATC 5 cut(s) 479, 845, 898, 1124, 1325
BceAI ACGGC 2 cut(s) 160, 1299
BcoDI GTCTC 1 cut(s) 606
BcuI ACTAGT 1 cut(s) 1207
BfaI CTAG 6 cut(s) 11, 602, 972, 1208, 1242, 1524
BfmI CTRYAG 2 cut(s) 173, 981
BfoI RGCGCY 2 cut(s) 273, 1513
BglII AGATCT 2 cut(s) 876, 1429
BisI GCNGC 5 cut(s) 362, 365, 368, 989, 1044
BlpI GCTNAGC 1 cut(s) 131
BlsI GCNGC 5 cut(s) 363, 366, 369, 990, 1045
Bme18I GGWCC 2 cut(s) 85, 854
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 3 cut(s) 85, 255, 854
BmiI GGNNCC 7 cut(s) 73, 87, 256, 271, 794, 1357, 1491
BmsI GCATC 3 cut(s) 37, 952, 958
BmtI GCTAGC 1 cut(s) 1527
BpiI GAAGAC 2 cut(s) 180, 221
BplI GAGNNNNNCTC 2 cut(s) 917, 949
BpmI CTGGAG 2 cut(s) 429, 846
Bpu10I CCTNAGC 1 cut(s) 747
Bpu1102I GCTNAGC 1 cut(s) 131
Bpu14I TTCGAA 3 cut(s) 345, 757, 1054
BpuEI CTTGAG 2 cut(s) 629, 1305
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 3 cut(s) 81, 409, 796
BsaWI WCCGGW 2 cut(s) 222, 452
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 452
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 4 cut(s) 501, 829, 1274, 1419
Bse3DI GCAATG 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 452
BseDI CCNNGG 3 cut(s) 81, 409, 796
BseGI GGATG 4 cut(s) 490, 967, 1086, 1336
BseLI CCNNNNNNNGG 2 cut(s) 312, 452
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 6 cut(s) 11, 122, 366, 566, 738, 1269
BseNI ACTGG 4 cut(s) 501, 829, 1274, 1419
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 5 cut(s) 354, 373, 376, 1000, 1055
BseYI CCCAGC 2 cut(s) 166, 778
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 1 cut(s) 1473
Bsh1285I CGRYCG 1 cut(s) 140
BshFI GGCC 4 cut(s) 5, 257, 1273, 1418
BshNI GGYRCC 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 140
BsiHKAI GWGCWC 3 cut(s) 185, 1146, 1321
BsiSI CCGG 3 cut(s) 6, 223, 453
BsiWI CGTACG 1 cut(s) 141
BslFI GGGAC 3 cut(s) 183, 739, 1012
BslI CCNNNNNNNGG 2 cut(s) 312, 452
BsmAI GTCTC 1 cut(s) 606
BsmFI GGGAC 3 cut(s) 183, 739, 1012
BsnI GGCC 4 cut(s) 5, 257, 1273, 1418
Bsp119I TTCGAA 3 cut(s) 345, 757, 1054
Bsp1286I GDGCHC 3 cut(s) 185, 1146, 1321
Bsp13I TCCGGA 1 cut(s) 452
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 9 cut(s) 71, 137, 333, 876, 886, 1066, 1133, 1355, 1429
Bsp1720I GCTNAGC 1 cut(s) 131
BspACI CCGC 2 cut(s) 517, 1471
BspANI GGCC 4 cut(s) 5, 257, 1273, 1418
BspCNI CTCAG 6 cut(s) 12, 123, 367, 567, 739, 1270
BspEI TCCGGA 1 cut(s) 452
BspFNI CGCG 1 cut(s) 1473
BspLI GGNNCC 7 cut(s) 73, 87, 256, 271, 794, 1357, 1491
BspMAI CTGCAG 2 cut(s) 177, 985
BspOI GCTAGC 1 cut(s) 1527
BspPI GGATC 7 cut(s) 66, 79, 881, 1061, 1141, 1350, 1363
BspT104I TTCGAA 3 cut(s) 345, 757, 1054
BspT107I GGYRCC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 711
BsrFI RCCGGY 1 cut(s) 222
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 4 cut(s) 501, 829, 1274, 1419
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 3 cut(s) 81, 409, 796
BssMI GATC 9 cut(s) 71, 137, 333, 876, 886, 1066, 1133, 1355, 1429
BssNAI GTATAC 1 cut(s) 1247
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 3 cut(s) 81, 409, 796
Bst1107I GTATAC 1 cut(s) 1247
Bst4CI ACNGT 5 cut(s) 121, 196, 513, 730, 1397
Bst6I CTCTTC 1 cut(s) 515
BstACI GRCGYC 1 cut(s) 270
BstAPI GCANNNNNTGC 2 cut(s) 367, 710
BstAUI TGTACA 1 cut(s) 150
BstBI TTCGAA 3 cut(s) 345, 757, 1054
BstC8I GCNNGC 9 cut(s) 274, 624, 685, 804, 949, 953, 1202, 1475, 1525
BstDEI CTNAG 8 cut(s) 20, 131, 375, 575, 747, 880, 1039, 1278
BstF5I GGATG 4 cut(s) 490, 967, 1086, 1336
BstFNI CGCG 1 cut(s) 1473
BstH2I RGCGCY 2 cut(s) 273, 1513
BstHHI GCGC 3 cut(s) 230, 272, 1512
BstKTI GATC 9 cut(s) 74, 140, 336, 879, 889, 1069, 1136, 1358, 1432
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 9 cut(s) 71, 137, 333, 876, 886, 1066, 1133, 1355, 1429
BstMCI CGRYCG 1 cut(s) 140
BstMWI GCNNNNNNNGC 3 cut(s) 172, 367, 710
BstNSI RCATGY 3 cut(s) 742, 1157, 1295
BstSFI CTRYAG 2 cut(s) 173, 981
BstSLI GKGCMC 1 cut(s) 185
BstUI CGCG 1 cut(s) 1473
BstV1I GCAGC 5 cut(s) 354, 373, 376, 1000, 1055
BstV2I GAAGAC 2 cut(s) 180, 221
BstX2I RGATCY 5 cut(s) 71, 876, 1133, 1355, 1429
BstYI RGATCY 5 cut(s) 71, 876, 1133, 1355, 1429
BstZ17I GTATAC 1 cut(s) 1247
BsuRI GGCC 4 cut(s) 5, 257, 1273, 1418
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 4 cut(s) 490, 967, 1086, 1336
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 3 cut(s) 283, 494, 1316
Cac8I GCNNGC 9 cut(s) 274, 624, 685, 804, 949, 953, 1202, 1475, 1525
CaiI CAGNNNCTG 1 cut(s) 367
CfoI GCGC 3 cut(s) 230, 272, 1512
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 3 cut(s) 85, 255, 854
Csp6I GTAC 6 cut(s) 142, 151, 605, 822, 1254, 1464
CspAI ACCGGT 1 cut(s) 222
CviAII CATG 8 cut(s) 236, 402, 428, 569, 587, 739, 1154, 1292
CviQI GTAC 6 cut(s) 142, 151, 605, 822, 1254, 1464
DdeI CTNAG 8 cut(s) 20, 131, 375, 575, 747, 880, 1039, 1278
DinI GGCGCC 1 cut(s) 271
DpnI GATC 9 cut(s) 73, 139, 335, 878, 888, 1068, 1135, 1357, 1431
DpnII GATC 9 cut(s) 71, 137, 333, 876, 886, 1066, 1133, 1355, 1429
DraIII CACNNNGTG 1 cut(s) 392
EaeI YGGCCR 2 cut(s) 3, 1416
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
Ecl136II GAGCTC 1 cut(s) 1144
Eco130I CCWWGG 3 cut(s) 81, 409, 796
Eco24I GRGCYC 1 cut(s) 1146
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 85, 854
Eco53kI GAGCTC 1 cut(s) 1144
Eco57I CTGAAG 1 cut(s) 1292
EcoICRI GAGCTC 1 cut(s) 1144
EcoRI GAATTC 2 cut(s) 694, 753
EcoRV GATATC 1 cut(s) 505
EcoT14I CCWWGG 3 cut(s) 81, 409, 796
EcoT38I GRGCYC 1 cut(s) 1146
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
ErhI CCWWGG 3 cut(s) 81, 409, 796
FaeI CATG 8 cut(s) 239, 405, 431, 572, 590, 742, 1157, 1295
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 3 cut(s) 183, 739, 1012
FatI CATG 8 cut(s) 235, 401, 427, 568, 586, 738, 1153, 1291
FblI GTMKAC 1 cut(s) 1246
Fnu4HI GCNGC 5 cut(s) 362, 365, 368, 989, 1044
FokI GGATG 4 cut(s) 497, 974, 1093, 1343
FriOI GRGCYC 1 cut(s) 1146
Fsp4HI GCNGC 5 cut(s) 362, 365, 368, 989, 1044
FspBI CTAG 6 cut(s) 11, 602, 972, 1208, 1242, 1524
GlaI GCGC 3 cut(s) 229, 271, 1511
GluI GCNGC 5 cut(s) 362, 365, 368, 989, 1044
GsaI CCCAGC 2 cut(s) 170, 782
GsuI CTGGAG 2 cut(s) 429, 846
HaeII RGCGCY 2 cut(s) 273, 1513
HaeIII GGCC 4 cut(s) 5, 257, 1273, 1418
HapII CCGG 3 cut(s) 6, 223, 453
HhaI GCGC 3 cut(s) 230, 272, 1512
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 8 cut(s) 239, 405, 431, 572, 590, 742, 1157, 1295
Hin6I GCGC 3 cut(s) 228, 270, 1510
HinP1I GCGC 3 cut(s) 228, 270, 1510
HindIII AAGCTT 3 cut(s) 560, 945, 1304
HinfI GANTC 4 cut(s) 95, 449, 1178, 1372
HpaII CCGG 3 cut(s) 6, 223, 453
HphI GGTGA 5 cut(s) 662, 885, 912, 1277, 1403
Hpy166II GTNNAC 6 cut(s) 151, 183, 199, 607, 1036, 1247
Hpy188I TCNGA 5 cut(s) 94, 112, 315, 558, 1138
Hpy188III TCNNGA 5 cut(s) 446, 453, 1277, 1328, 1353
Hpy8I GTNNAC 6 cut(s) 151, 183, 199, 607, 1036, 1247
HpyAV CCTTC 6 cut(s) 49, 912, 952, 1015, 1078, 1369
HpyCH4III ACNGT 5 cut(s) 121, 196, 513, 730, 1397
HpyCH4IV ACGT 2 cut(s) 185, 1163
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 367, 710
HpyF3I CTNAG 8 cut(s) 20, 131, 375, 575, 747, 880, 1039, 1278
HpySE526I ACGT 2 cut(s) 185, 1163
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 8 cut(s) 239, 405, 431, 572, 590, 742, 1157, 1295
HspAI GCGC 3 cut(s) 228, 270, 1510
KasI GGCGCC 1 cut(s) 269
Kpn2I TCCGGA 1 cut(s) 452
Kzo9I GATC 9 cut(s) 71, 137, 333, 876, 886, 1066, 1133, 1355, 1429
LmnI GCTCC 2 cut(s) 792, 1489
Lsp1109I GCAGC 5 cut(s) 354, 373, 376, 1000, 1055
LweI GCATC 3 cut(s) 37, 952, 958
MaeI CTAG 6 cut(s) 11, 602, 972, 1208, 1242, 1524
MaeII ACGT 2 cut(s) 185, 1163
MaeIII GTNAC 5 cut(s) 404, 492, 730, 1265, 1391
MalI GATC 9 cut(s) 73, 139, 335, 878, 888, 1068, 1135, 1357, 1431
MboI GATC 9 cut(s) 71, 137, 333, 876, 886, 1066, 1133, 1355, 1429
MboII GAAGA 6 cut(s) 26, 180, 226, 532, 537, 1187
MfeI CAATTG 1 cut(s) 1518
MflI RGATCY 5 cut(s) 71, 876, 1133, 1355, 1429
MhlI GDGCHC 3 cut(s) 185, 1146, 1321
MlsI TGGCCA 1 cut(s) 1418
MluCI AATT 7 cut(s) 628, 694, 753, 766, 830, 1341, 1518
MluNI TGGCCA 1 cut(s) 1418
Mly113I GGCGCC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 1094
Mox20I TGGCCA 1 cut(s) 1418
MroI TCCGGA 1 cut(s) 452
MroXI GAANNNNTTC 2 cut(s) 1305, 1376
MscI TGGCCA 1 cut(s) 1418
MseI TTAA 2 cut(s) 326, 1425
Msp20I TGGCCA 1 cut(s) 1418
MspA1I CMGCKG 2 cut(s) 367, 782
MspI CCGG 3 cut(s) 6, 223, 453
MunI CAATTG 1 cut(s) 1518
MvnI CGCG 1 cut(s) 1473
MwoI GCNNNNNNNGC 3 cut(s) 172, 367, 710
NarI GGCGCC 1 cut(s) 270
NdeII GATC 9 cut(s) 71, 137, 333, 876, 886, 1066, 1133, 1355, 1429
NheI GCTAGC 1 cut(s) 1523
NlaIII CATG 8 cut(s) 239, 405, 431, 572, 590, 742, 1157, 1295
NlaIV GGNNCC 7 cut(s) 73, 87, 256, 271, 794, 1357, 1491
NmuCI GTSAC 3 cut(s) 492, 1265, 1391
NspI RCATGY 3 cut(s) 742, 1157, 1295
NspV TTCGAA 3 cut(s) 345, 757, 1054
PciI ACATGT 1 cut(s) 738
PdmI GAANNNNTTC 2 cut(s) 1305, 1376
PfeI GAWTC 4 cut(s) 95, 449, 1178, 1372
Pfl23II CGTACG 1 cut(s) 141
PinAI ACCGGT 1 cut(s) 222
PkrI GCNGC 5 cut(s) 363, 366, 369, 990, 1045
Ple19I CGATCG 1 cut(s) 140
PluTI GGCGCC 1 cut(s) 273
PscI ACATGT 1 cut(s) 738
PsiI TTATAA 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 1146
PspFI CCCAGC 2 cut(s) 166, 778
PspLI CGTACG 1 cut(s) 141
PspN4I GGNNCC 7 cut(s) 73, 87, 256, 271, 794, 1357, 1491
PspPI GGNCC 3 cut(s) 85, 255, 854
PstI CTGCAG 2 cut(s) 177, 985
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 5 cut(s) 71, 876, 1133, 1355, 1429
PvuI CGATCG 1 cut(s) 140
PvuII CAGCTG 2 cut(s) 367, 782
RsaI GTAC 6 cut(s) 143, 152, 606, 823, 1255, 1465
RsaNI GTAC 6 cut(s) 142, 151, 605, 822, 1254, 1464
SacI GAGCTC 1 cut(s) 1146
SaqAI TTAA 2 cut(s) 326, 1425
SatI GCNGC 5 cut(s) 362, 365, 368, 989, 1044
Sau3AI GATC 9 cut(s) 71, 137, 333, 876, 886, 1066, 1133, 1355, 1429
Sau96I GGNCC 3 cut(s) 85, 255, 854
SduI GDGCHC 3 cut(s) 185, 1146, 1321
SfaNI GCATC 3 cut(s) 37, 952, 958
SfcI CTRYAG 2 cut(s) 173, 981
SfoI GGCGCC 1 cut(s) 271
SfuI TTCGAA 3 cut(s) 345, 757, 1054
SinI GGWCC 2 cut(s) 85, 854
SmlI CTYRAG 2 cut(s) 644, 1320
SmoI CTYRAG 2 cut(s) 644, 1320
SpeI ACTAGT 1 cut(s) 1207
Sse9I AATT 7 cut(s) 628, 694, 753, 766, 830, 1341, 1518
SsiI CCGC 2 cut(s) 517, 1471
SspDI GGCGCC 1 cut(s) 269
SspMI CTAG 6 cut(s) 11, 602, 972, 1208, 1242, 1524
SstI GAGCTC 1 cut(s) 1146
StyI CCWWGG 3 cut(s) 81, 409, 796
TaaI ACNGT 5 cut(s) 121, 196, 513, 730, 1397
TaiI ACGT 2 cut(s) 188, 1166
TaqI TCGA 5 cut(s) 45, 345, 757, 1054, 1448
TasI AATT 7 cut(s) 628, 694, 753, 766, 830, 1341, 1518
TatI WGTACW 3 cut(s) 150, 604, 1253
TfiI GAWTC 4 cut(s) 95, 449, 1178, 1372
Tru1I TTAA 2 cut(s) 326, 1425
Tru9I TTAA 2 cut(s) 326, 1425
TscAI CASTG 3 cut(s) 283, 501, 1316
TseFI GTSAC 3 cut(s) 492, 1265, 1391
TseI GCWGC 5 cut(s) 361, 364, 367, 988, 1043
Tsp45I GTSAC 3 cut(s) 492, 1265, 1391
TspDTI ATGAA 7 cut(s) 87, 416, 997, 1097, 1188, 1351, 1471
TspRI CASTG 3 cut(s) 283, 501, 1316
VneI GTGCAC 1 cut(s) 181
VpaK11BI GGWCC 2 cut(s) 85, 854
XapI RAATTY 3 cut(s) 694, 753, 830
XceI RCATGY 3 cut(s) 742, 1157, 1295
XcmI CCANNNNNNNNNTGG 1 cut(s) 786
XmiI GTMKAC 1 cut(s) 1246
XmnI GAANNNNTTC 2 cut(s) 1305, 1376
XspI CTAG 6 cut(s) 11, 602, 972, 1208, 1242, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.