Rh2DG667400

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005843
Physical Location & Seq
Reverse (-)
89177113 .. 89180968
3856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG667400.1

Sequence Viewer

Length: 1044 bp
ATGGCCGGACTAGAAGAACTGAGGAAAAAGCTGACACCTTTGTTCGATGCAGAGAAGGGTCTGGGCTTGGGATCCTCCACTTTGGACCCGTCAGATTCATACACACTTTCGGACAGAACGGTGGAGTTGCTGAGCCGATCGTACGGCGTGTACAACATCAACGAGCTGGGACTGCAGAAGTGCACGTCTTCTACGGTGGACGACACCACAGAGAAGACTTACCGGTGCGCTTCGCATGAGATGAGGGTGTTTGGGGCCATTGGGAGTGGCGCCAGCAGTGTTGTGCAGAGAGCTATTCACATTCCCACTCATCGGATTATAGCGTTAAAAAAGATCAACATTTTCGAAAAGGAAAAAAGGCAGCAGCTGCTTACTGAGATAAGGACACTATGTGAAGCACCTTGTTACCAAGGTCTTGTAGAGTTTCATGGTGCATTTTATACTCCAGATTCCGGACAGATAAGCATAGCTTTGGAGTATATGGATGGAGGGTCACTGGCAGATATCTTACGGTTGCGGAAGAGAATACCCGAACCTCTACTTTCTTCTATGTTTCAGAAGCTTCTACATGGACTGAGCTACTTGCATGGAGTTAGGTATCTAGTACACAGAGACATAAAGCCTGCCAATTTGCTTATAAATCTCAAGGGTGAGCCAAAGATAACAGACTTCGGTATAAGTGCTGGCTTGGAGAATTCTATGGCAATGTGTGCAACATTTGTTGGGACAGTTACATACATGTCGCCTGAGCGAATTCGAAATGAGAATTATTCTTACCCAGCTGATATTTGGAGCCTTGGGCTTGCTCTCTTTGAGTGTGGTACTGGAGAATTTCCATATACAGCTAATGATGGACCTGTCAATCTTATGTTGCAGATCTTAGATGATCCATCACCTACACCTTCAAAACAGAACTTTTCACCAGAGTTCTGCTCGTTTATTGAAGCTTGCTTGCAGAAGGATGCAGATGCTAGACCAACTGCAGAGCAGGTGATCAAATACCATAGATTAGCCAGGCTATACAATATAGACATGATAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001101 GO:0001932 GO:0001934 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009753 GO:0009755 GO:0009791 GO:0009814 GO:0009864 GO:0009866 GO:0009867 GO:0009873 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033206 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04016 ko05418 map04016 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

347

Amino Acids

38.65

Weight (kDa)

5.95

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 84 - 333 9.3e-58 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 86 - 332 2.9e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AarI CACCTGC 1 cut(s) 979
Acc36I ACCTGC 1 cut(s) 979
AccB1I GGYRCC 1 cut(s) 269
AccIII TCCGGA 1 cut(s) 452
AciI CCGC 1 cut(s) 517
AclWI GGATC 3 cut(s) 66, 79, 881
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 694, 753, 830
AcyI GRCGYC 1 cut(s) 270
AdeI CACNNNGTG 1 cut(s) 392
AfaI GTAC 4 cut(s) 143, 152, 606, 823
AfiI CCNNNNNNNGG 2 cut(s) 312, 452
AflIII ACRYGT 1 cut(s) 738
AgeI ACCGGT 1 cut(s) 222
AgsI TTSAA 2 cut(s) 906, 944
AjiI CACGTC 1 cut(s) 186
AjnI CCWGG 1 cut(s) 1013
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 606
Alw44I GTGCAC 1 cut(s) 181
AlwI GGATC 3 cut(s) 66, 79, 881
AlwNI CAGNNNCTG 1 cut(s) 367
Aor13HI TCCGGA 1 cut(s) 452
AoxI GGCC 2 cut(s) 3, 255
ApaLI GTGCAC 1 cut(s) 181
ApeKI GCWGC 3 cut(s) 361, 364, 367
ApoI RAATTY 3 cut(s) 694, 753, 830
AsiGI ACCGGT 1 cut(s) 222
AspLEI GCGC 2 cut(s) 230, 272
AspS9I GGNCC 3 cut(s) 85, 255, 854
AsuHPI GGTGA 4 cut(s) 662, 885, 912, 1003
AsuII TTCGAA 2 cut(s) 345, 757
AvaII GGWCC 2 cut(s) 85, 854
BaeGI GKGCMC 1 cut(s) 185
BamHI GGATCC 1 cut(s) 71
BanI GGYRCC 1 cut(s) 269
BbsI GAAGAC 2 cut(s) 180, 221
Bbv12I GWGCWC 1 cut(s) 185
BbvI GCAGC 3 cut(s) 354, 373, 376
BccI CCATC 3 cut(s) 479, 845, 898
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 1 cut(s) 1015
BclI TGATCA 1 cut(s) 993
BcoDI GTCTC 1 cut(s) 606
BfaI CTAG 3 cut(s) 11, 602, 972
BfmI CTRYAG 2 cut(s) 173, 981
BfoI RGCGCY 1 cut(s) 273
BfuAI ACCTGC 1 cut(s) 979
BglII AGATCT 1 cut(s) 876
BisI GCNGC 3 cut(s) 362, 365, 368
BlpI GCTNAGC 1 cut(s) 131
BlsI GCNGC 3 cut(s) 363, 366, 369
Bme1390I CCNGG 1 cut(s) 1015
Bme18I GGWCC 2 cut(s) 85, 854
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 3 cut(s) 85, 255, 854
BmiI GGNNCC 5 cut(s) 73, 87, 256, 271, 794
BmrFI CCNGG 1 cut(s) 1015
BmsI GCATC 3 cut(s) 37, 952, 958
BpiI GAAGAC 2 cut(s) 180, 221
BplI GAGNNNNNCTC 2 cut(s) 917, 949
BpmI CTGGAG 2 cut(s) 429, 846
Bpu10I CCTNAGC 1 cut(s) 747
Bpu1102I GCTNAGC 1 cut(s) 131
Bpu14I TTCGAA 2 cut(s) 345, 757
BpuEI CTTGAG 1 cut(s) 629
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 2 cut(s) 409, 796
BsaWI WCCGGW 2 cut(s) 222, 452
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 452
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 2 cut(s) 501, 829
Bse3DI GCAATG 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 452
BseBI CCWGG 1 cut(s) 1015
BseDI CCNNGG 2 cut(s) 409, 796
BseGI GGATG 2 cut(s) 490, 967
BseLI CCNNNNNNNGG 2 cut(s) 312, 452
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 5 cut(s) 11, 122, 366, 566, 738
BseNI ACTGG 2 cut(s) 501, 829
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 3 cut(s) 354, 373, 376
BseYI CCCAGC 2 cut(s) 166, 778
BsgI GTGCAG 1 cut(s) 305
Bsh1285I CGRYCG 1 cut(s) 140
BshFI GGCC 2 cut(s) 5, 257
BshNI GGYRCC 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 3 cut(s) 6, 223, 453
BsiWI CGTACG 1 cut(s) 141
BslFI GGGAC 2 cut(s) 183, 739
BslI CCNNNNNNNGG 2 cut(s) 312, 452
BsmAI GTCTC 1 cut(s) 606
BsmFI GGGAC 2 cut(s) 183, 739
BsnI GGCC 2 cut(s) 5, 257
Bsp119I TTCGAA 2 cut(s) 345, 757
Bsp1286I GDGCHC 1 cut(s) 185
Bsp13I TCCGGA 1 cut(s) 452
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 6 cut(s) 71, 137, 333, 876, 886, 993
Bsp1720I GCTNAGC 1 cut(s) 131
BspACI CCGC 1 cut(s) 517
BspANI GGCC 2 cut(s) 5, 257
BspCNI CTCAG 5 cut(s) 12, 123, 367, 567, 739
BspEI TCCGGA 1 cut(s) 452
BspLI GGNNCC 5 cut(s) 73, 87, 256, 271, 794
BspMAI CTGCAG 2 cut(s) 177, 985
BspMI ACCTGC 1 cut(s) 979
BspPI GGATC 3 cut(s) 66, 79, 881
BspT104I TTCGAA 2 cut(s) 345, 757
BspT107I GGYRCC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 711
BsrFI RCCGGY 1 cut(s) 222
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 2 cut(s) 501, 829
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 2 cut(s) 409, 796
BssMI GATC 6 cut(s) 71, 137, 333, 876, 886, 993
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 2 cut(s) 409, 796
Bst2UI CCWGG 1 cut(s) 1015
Bst4CI ACNGT 4 cut(s) 121, 196, 513, 730
Bst6I CTCTTC 1 cut(s) 515
BstACI GRCGYC 1 cut(s) 270
BstAPI GCANNNNNTGC 2 cut(s) 367, 710
BstAUI TGTACA 1 cut(s) 150
BstBI TTCGAA 2 cut(s) 345, 757
BstC8I GCNNGC 6 cut(s) 274, 624, 685, 804, 949, 953
BstDEI CTNAG 6 cut(s) 20, 131, 375, 575, 747, 880
BstF5I GGATG 2 cut(s) 490, 967
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 2 cut(s) 230, 272
BstKTI GATC 6 cut(s) 74, 140, 336, 879, 889, 996
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 6 cut(s) 71, 137, 333, 876, 886, 993
BstMCI CGRYCG 1 cut(s) 140
BstMWI GCNNNNNNNGC 3 cut(s) 172, 367, 710
BstNI CCWGG 1 cut(s) 1015
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 1013
BstSFI CTRYAG 2 cut(s) 173, 981
BstSLI GKGCMC 1 cut(s) 185
BstV1I GCAGC 3 cut(s) 354, 373, 376
BstV2I GAAGAC 2 cut(s) 180, 221
BstX2I RGATCY 2 cut(s) 71, 876
BstYI RGATCY 2 cut(s) 71, 876
BsuRI GGCC 2 cut(s) 5, 257
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 2 cut(s) 490, 967
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 283, 494
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 6 cut(s) 274, 624, 685, 804, 949, 953
CaiI CAGNNNCTG 1 cut(s) 367
CfoI GCGC 2 cut(s) 230, 272
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 3 cut(s) 85, 255, 854
Csp6I GTAC 4 cut(s) 142, 151, 605, 822
CspAI ACCGGT 1 cut(s) 222
CviAII CATG 6 cut(s) 236, 428, 569, 587, 739, 1033
CviQI GTAC 4 cut(s) 142, 151, 605, 822
DdeI CTNAG 6 cut(s) 20, 131, 375, 575, 747, 880
DinI GGCGCC 1 cut(s) 271
DpnI GATC 6 cut(s) 73, 139, 335, 878, 888, 995
DpnII GATC 6 cut(s) 71, 137, 333, 876, 886, 993
DraIII CACNNNGTG 1 cut(s) 392
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
Eco130I CCWWGG 2 cut(s) 409, 796
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 85, 854
EcoRI GAATTC 2 cut(s) 694, 753
EcoRII CCWGG 1 cut(s) 1013
EcoRV GATATC 1 cut(s) 505
EcoT14I CCWWGG 2 cut(s) 409, 796
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
ErhI CCWWGG 2 cut(s) 409, 796
FaeI CATG 6 cut(s) 239, 431, 572, 590, 742, 1036
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 2 cut(s) 183, 739
FatI CATG 6 cut(s) 235, 427, 568, 586, 738, 1032
FbaI TGATCA 1 cut(s) 993
Fnu4HI GCNGC 3 cut(s) 362, 365, 368
FokI GGATG 2 cut(s) 497, 974
Fsp4HI GCNGC 3 cut(s) 362, 365, 368
FspBI CTAG 3 cut(s) 11, 602, 972
GlaI GCGC 2 cut(s) 229, 271
GluI GCNGC 3 cut(s) 362, 365, 368
GsaI CCCAGC 2 cut(s) 170, 782
GsuI CTGGAG 2 cut(s) 429, 846
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 2 cut(s) 5, 257
HapII CCGG 3 cut(s) 6, 223, 453
HhaI GCGC 2 cut(s) 230, 272
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 6 cut(s) 239, 431, 572, 590, 742, 1036
Hin6I GCGC 2 cut(s) 228, 270
HinP1I GCGC 2 cut(s) 228, 270
HindIII AAGCTT 2 cut(s) 560, 945
HinfI GANTC 2 cut(s) 95, 449
HpaII CCGG 3 cut(s) 6, 223, 453
HphI GGTGA 4 cut(s) 662, 885, 912, 1003
Hpy166II GTNNAC 4 cut(s) 151, 183, 199, 607
Hpy188I TCNGA 4 cut(s) 94, 112, 315, 558
Hpy188III TCNNGA 2 cut(s) 446, 453
Hpy8I GTNNAC 4 cut(s) 151, 183, 199, 607
HpyAV CCTTC 3 cut(s) 49, 912, 952
HpyCH4III ACNGT 4 cut(s) 121, 196, 513, 730
HpyCH4IV ACGT 1 cut(s) 185
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 367, 710
HpyF3I CTNAG 6 cut(s) 20, 131, 375, 575, 747, 880
HpySE526I ACGT 1 cut(s) 185
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 6 cut(s) 239, 431, 572, 590, 742, 1036
HspAI GCGC 2 cut(s) 228, 270
KasI GGCGCC 1 cut(s) 269
Kpn2I TCCGGA 1 cut(s) 452
Ksp22I TGATCA 1 cut(s) 993
Kzo9I GATC 6 cut(s) 71, 137, 333, 876, 886, 993
LmnI GCTCC 1 cut(s) 792
Lsp1109I GCAGC 3 cut(s) 354, 373, 376
LweI GCATC 3 cut(s) 37, 952, 958
MaeI CTAG 3 cut(s) 11, 602, 972
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 3 cut(s) 404, 492, 730
MalI GATC 6 cut(s) 73, 139, 335, 878, 888, 995
MboI GATC 6 cut(s) 71, 137, 333, 876, 886, 993
MboII GAAGA 5 cut(s) 26, 180, 226, 532, 537
MflI RGATCY 2 cut(s) 71, 876
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 5 cut(s) 628, 694, 753, 766, 830
Mly113I GGCGCC 1 cut(s) 270
MnlI CCTC 5 cut(s) 15, 85, 237, 482, 546
MroI TCCGGA 1 cut(s) 452
MseI TTAA 1 cut(s) 326
MspA1I CMGCKG 2 cut(s) 367, 782
MspI CCGG 3 cut(s) 6, 223, 453
MspR9I CCNGG 1 cut(s) 1015
MvaI CCWGG 1 cut(s) 1015
MwoI GCNNNNNNNGC 3 cut(s) 172, 367, 710
NarI GGCGCC 1 cut(s) 270
NdeII GATC 6 cut(s) 71, 137, 333, 876, 886, 993
NlaIII CATG 6 cut(s) 239, 431, 572, 590, 742, 1036
NlaIV GGNNCC 5 cut(s) 73, 87, 256, 271, 794
NmuCI GTSAC 1 cut(s) 492
NspI RCATGY 1 cut(s) 742
NspV TTCGAA 2 cut(s) 345, 757
PaqCI CACCTGC 1 cut(s) 979
PciI ACATGT 1 cut(s) 738
PfeI GAWTC 2 cut(s) 95, 449
Pfl23II CGTACG 1 cut(s) 141
PinAI ACCGGT 1 cut(s) 222
PkrI GCNGC 3 cut(s) 363, 366, 369
Ple19I CGATCG 1 cut(s) 140
PluTI GGCGCC 1 cut(s) 273
PscI ACATGT 1 cut(s) 738
PsiI TTATAA 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 1013
PspFI CCCAGC 2 cut(s) 166, 778
PspGI CCWGG 1 cut(s) 1013
PspLI CGTACG 1 cut(s) 141
PspN4I GGNNCC 5 cut(s) 73, 87, 256, 271, 794
PspPI GGNCC 3 cut(s) 85, 255, 854
PstI CTGCAG 2 cut(s) 177, 985
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 2 cut(s) 71, 876
PvuI CGATCG 1 cut(s) 140
PvuII CAGCTG 2 cut(s) 367, 782
RsaI GTAC 4 cut(s) 143, 152, 606, 823
RsaNI GTAC 4 cut(s) 142, 151, 605, 822
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 3 cut(s) 362, 365, 368
Sau3AI GATC 6 cut(s) 71, 137, 333, 876, 886, 993
Sau96I GGNCC 3 cut(s) 85, 255, 854
ScrFI CCNGG 1 cut(s) 1015
SduI GDGCHC 1 cut(s) 185
SfaNI GCATC 3 cut(s) 37, 952, 958
SfcI CTRYAG 2 cut(s) 173, 981
SfoI GGCGCC 1 cut(s) 271
SfuI TTCGAA 2 cut(s) 345, 757
SinI GGWCC 2 cut(s) 85, 854
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 5 cut(s) 628, 694, 753, 766, 830
SsiI CCGC 1 cut(s) 517
SspDI GGCGCC 1 cut(s) 269
SspMI CTAG 3 cut(s) 11, 602, 972
StyD4I CCNGG 1 cut(s) 1013
StyI CCWWGG 2 cut(s) 409, 796
TaaI ACNGT 4 cut(s) 121, 196, 513, 730
TaiI ACGT 1 cut(s) 188
TaqI TCGA 3 cut(s) 45, 345, 757
TasI AATT 5 cut(s) 628, 694, 753, 766, 830
TatI WGTACW 2 cut(s) 150, 604
TfiI GAWTC 2 cut(s) 95, 449
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TscAI CASTG 2 cut(s) 283, 501
TseFI GTSAC 1 cut(s) 492
TseI GCWGC 3 cut(s) 361, 364, 367
Tsp45I GTSAC 1 cut(s) 492
TspDTI ATGAA 2 cut(s) 87, 416
TspRI CASTG 2 cut(s) 283, 501
VneI GTGCAC 1 cut(s) 181
VpaK11BI GGWCC 2 cut(s) 85, 854
XapI RAATTY 3 cut(s) 694, 753, 830
XceI RCATGY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 786
XspI CTAG 3 cut(s) 11, 602, 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.