FvH4_7g01210

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014942
Physical Location & Seq
Reverse (-)
1625119 .. 1629507
4389 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01210.t2

Sequence Viewer

Length: 2391 bp
ATGGAGGTGAGAAACAGAAAAGAGGGTGGTGTTGATACAAATGACATTCCTGATGGATGCTCAGTACAATTAACAAAGCTTATCACACATGTAGCCATTCATGTTGCAGGTTTCTTCTTAATGTACATTCTCTGTCATCTTTCGCACTACCTGCTAAAGCCTTATTCCCAGCCTCGCATTACCTCTGATACCTTTGTAGGACTAGTCATCGGAAACATAGGGTTCATACGTGGTGTTTTATCACAGTCATCAAAGCATATATTGCGTTACCTGGTTGAATTTGGAATGATTGGCTACATGCTTGTGCTAGGAATAGAAATGGATCCATATGTGCTTTTGAAACTACCAACCAAAGACGCGAAAGTGGCCTATGTTGGTATGCTCTCCACATTCATCCTAGCCTGTGGAATAACCCCATTTCTGCACTATACAGAAAGTTTTAAGATTGACTTCACTCTCTCCCTCTCCATGGTCCTTTCGAGCACGGCTTCGCCTGTACTGACACGAATAATAACTAGTCTCAAAATAGGCAAGTCAGACATAGGCCAGCTCGTCATTGCTACAGGAATGCACTCTGATTTTATAGCCACTCTTCTCCTCTCCATTGGCTTTGTCATTTGGCCTGTTGAAGATGCAGATAAAAAAAGCAGCCTTCATAGATCCCTTGAGATGGGTATTGCATTGTTGATCACGGCAGTTTTTACAGCCTTTGTTTCACCAATCTTCATGAACTGGGTTAACAATGAAAACCCCGAAGGCAAACCCATGAAAGGCTCACATCTAGTCCTCTCTATGGCTTTCATGGCCTTCGCTGTGGCCTGCCCATCATATTTTGGGTACAGTTCTATTTTGAGTGCATTTATGGCAGGAATCTTTTTACCAAGGGAAGGAAGAGTGTCAAAGTGGGTGATAGGCAAGATCAACTACTTGTTGAGTACCATATACTATCCCATTTTTTTCTTTTGGATGGGGTATGAAGCTTATTTTGCAGAATTCCAGCCTCTTGACTGGATGACATGGGCAAGGTTATTTGTTGTTTTTGCTATAGCAACAGTCGGAAAAGTTTTAGGAACAGTCATCTCTGGAGCCCTCTTGGGGTTTAACTGGCCTGAATCTGTTGCACTAGGGCTTCTTCTCACAACCAAAGGCCATTTTCATATATACTTGGCCATTGCTGCAAAATCGTATGGAAAAACCTCTACTTCAACCAGTACTGTGATGGTAATAGCTATTTTTTTCACTGTGGTGCACGCCCCGTCAGTTGTGGCACAAATTATTAAGCGTGCAAGGAGACGAGCACCCACTCATCGGATGTCACTTCAGTTGCTTGACCCCTCAAGCGAACTACGCATATTACTATGCCTTCATGGGCCACAAAATGTGCGTACTGCGATTAACTTCATTGAGCTATGTAGAGGGACAGCAGACCCTGGAGTTGTAGTATTTGTAACAGACATGATAGAACTTACGGACCAAATAGCAGCAACACTAGTTAAAGAACAAGGAGTGAACACAATGACTGTGACTGAAAAGGCGGTAACAGACATGAGAGCTCAAGTCACTGCTGCAATTCAATCCTATGTAGAAGAAGACGATAATGGTATTACACTAGGACGATTGCTTGCACTCTCGACTTTCAATGGCATGCCCCAAGATATATGCGTTTTGGCTGAGGACTCGACGGTGGCCCTCATCATATTGCCATTTCACAAGACACAAAGTGCAGATGGGACATTAGATGGCGGTCATTCTGGTTTCAGATTTGTGAACCGTAAGGTCCTCAAAAATGCTCCATGCTCCATAGGAATTCTTGTAGACAGAGGTTTTGGAATGATTGAAAAAATATCAAGATCTTATGATCATTCTATCGATATGGCTGTCATCTTCATTGGTGGAAAAGATGATAGAGAAGCACTCGCCTATGCCGGTCTTGTAGCACGACATCCTGGTGTAAAACTCACCATCTTAAGATTTTTGGTGGACACTACCACAAATGCGTCAAGTAGACCTGGCCATTACCCGGTCAGCCGTGCTCTACAAGAAGAGGAAATGAAGTTTGATGATGAGTGCTTTGCTAAGTTCTACGAGAGACATGTTGCATGTGGGCAAGTTGCTTACATGGAGAAGCATCTTGCCAATTCTTCTGAGACTTATAATACCTTGATGGCATTAGATGGGCAGTACTCACTTATAATAGTGGGAAGAGGGGAAAGGGTGAATACAGTTTTGACGTTTGGGATGAATGACTGGCAGCAGTGTCCGGAGTTGGGTCCGATTGGTGATGTTCTTGCGGGTTCTGAATTTTCAGTCAGAACATCAGTTTTAATCATTCAACAAAACAGTGTAAAAGGAGAACTAGACGGCCTTGATGATGACTTTTCTGTCATGTAA

Protein Analysis

797

Amino Acids

87.71

Weight (kDa)

6.41

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 20 - 383 3.7e-34 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 436 - 573 4.9e-13 Plant cation/H(+) antiporter domain
CHX17_C PF23259 579 - 738 1.7e-13 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014942)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52080
fragaria_vesca FvH4_7g01210 FvH4_7g01210
malus_domestica MD02G1308800.v1.1 MD07G1013200.v1.1
prunus_persica Prupe.2G016700_v2.0.a1 Prupe.2G016700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0316681
rosa_laevigata RLG00000030644
rosa_multiflora Rmu_sc0005539.1_g000005
rosa_roxburghii Rroxscaffold_4G00330860
rosa_samantha Rh1AG022700 Rh1BG021200 Rh1CG019800 Rh1DG017200
rosa_wichuraiana Rw1G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2154, 2192
AasI GACNNNNNNGTC 1 cut(s) 2381
Acc36I ACCTGC 2 cut(s) 98, 159
AccI GTMKAC 2 cut(s) 1815, 2005
AccII CGCG 1 cut(s) 359
AccIII TCCGGA 1 cut(s) 2260
AciI CCGC 3 cut(s) 1535, 1743, 2291
AclWI GGATC 3 cut(s) 317, 330, 654
AcoI YGGCCR 2 cut(s) 1167, 2011
AcsI RAATTY 4 cut(s) 278, 992, 1806, 2300
AcuI CTGAAG 1 cut(s) 1304
AdeI CACNNNGTG 1 cut(s) 1721
AfaI GTAC 8 cut(s) 66, 125, 498, 839, 937, 1213, 1387, 2183
AfiI CCNNNNNNNGG 9 cut(s) 469, 670, 770, 793, 887, 1095, 1308, 2020, 2267
AflII CTTAAG 1 cut(s) 1966
AflIII ACRYGT 2 cut(s) 88, 2092
AgsI TTSAA 8 cut(s) 278, 340, 629, 1206, 1574, 1639, 1838, 2333
AhlI ACTAGT 3 cut(s) 202, 515, 1489
AjnI CCWGG 4 cut(s) 270, 1429, 1945, 2008
AleI CACNNNNGTG 1 cut(s) 1244
AluBI AGCT 6 cut(s) 79, 550, 980, 1229, 1408, 1553
AluI AGCT 6 cut(s) 79, 550, 980, 1229, 1408, 1553
Alw21I GWGCWC 5 cut(s) 485, 1251, 1300, 1555, 2035
Alw26I GTCTC 4 cut(s) 524, 1285, 2083, 2141
Alw44I GTGCAC 1 cut(s) 1247
AlwI GGATC 3 cut(s) 317, 330, 654
AlwNI CAGNNNCTG 1 cut(s) 1430
Aor13HI TCCGGA 1 cut(s) 2260
ApaLI GTGCAC 1 cut(s) 1247
ApeKI GCWGC 5 cut(s) 648, 1175, 1481, 1565, 2251
ApoI RAATTY 4 cut(s) 278, 992, 1806, 2300
ArsI GACNNNNNNTTYG 2 cut(s) 1808, 1840
AspS9I GGNCC 6 cut(s) 472, 1370, 1471, 1687, 1777, 2270
AsuC2I CCSGG 1 cut(s) 2021
AsuHPI GGTGA 6 cut(s) 19, 708, 919, 1951, 2227, 2291
AvaII GGWCC 4 cut(s) 472, 1471, 1777, 2270
BaeGI GKGCMC 1 cut(s) 1251
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BalI TGGCCA 2 cut(s) 1169, 2013
BamHI GGATCC 1 cut(s) 322
BanII GRGCYC 2 cut(s) 1090, 1555
BbsI GAAGAC 1 cut(s) 1596
Bbv12I GWGCWC 5 cut(s) 485, 1251, 1300, 1555, 2035
BbvCI CCTCAGC 1 cut(s) 1671
BbvI GCAGC 5 cut(s) 660, 1162, 1493, 1552, 2263
BceAI ACGGC 4 cut(s) 501, 708, 2013, 2377
BciT130I CCWGG 4 cut(s) 272, 1431, 1947, 2010
BclI TGATCA 2 cut(s) 687, 1858
BcnI CCSGG 1 cut(s) 2021
BcoDI GTCTC 4 cut(s) 524, 1285, 2083, 2141
BcuI ACTAGT 3 cut(s) 202, 515, 1489
BfaI CTAG 9 cut(s) 203, 308, 398, 516, 782, 1124, 1490, 1610, 2357
BfmI CTRYAG 2 cut(s) 561, 1044
BfrI CTTAAG 1 cut(s) 1966
BfuAI ACCTGC 2 cut(s) 98, 159
BglII AGATCT 1 cut(s) 1850
BisI GCNGC 5 cut(s) 649, 1176, 1482, 1566, 2252
BlsI GCNGC 5 cut(s) 650, 1177, 1483, 1567, 2253
BmcAI AGTACT 2 cut(s) 1213, 2183
Bme1390I CCNGG 5 cut(s) 272, 1431, 1947, 2010, 2021
Bme18I GGWCC 4 cut(s) 472, 1471, 1777, 2270
BmgT120I GGNCC 6 cut(s) 472, 1370, 1471, 1687, 1777, 2270
BmiI GGNNCC 3 cut(s) 324, 1087, 2271
BmrFI CCNGG 5 cut(s) 272, 1431, 1947, 2010, 2021
BmrI ACTGGG 1 cut(s) 742
BmsI GCATC 3 cut(s) 47, 622, 2137
BmuI ACTGGG 1 cut(s) 742
BpiI GAAGAC 1 cut(s) 1596
BplI GAGNNNNNCTC 2 cut(s) 1899, 1931
BpmI CTGGAG 2 cut(s) 1104, 1452
Bpu10I CCTNAGC 1 cut(s) 1671
BpuEI CTTGAG 3 cut(s) 686, 1321, 1539
BpuMI CCSGG 1 cut(s) 2021
Bsa29I ATCGAT 1 cut(s) 1869
BsaAI YACGTR 1 cut(s) 230
BsaJI CCNNGG 3 cut(s) 468, 881, 1429
BsaWI WCCGGW 1 cut(s) 2260
BsaXI ACNNNNNCTCC 2 cut(s) 1425, 1455
Bsc4I CCNNNNNNNGG 9 cut(s) 469, 670, 770, 793, 887, 1095, 1308, 2020, 2267
Bse118I RCCGGY 1 cut(s) 1925
Bse1I ACTGG 5 cut(s) 737, 1013, 1109, 1209, 2252
Bse3DI GCAATG 2 cut(s) 555, 1170
BseAI TCCGGA 1 cut(s) 2260
BseBI CCWGG 4 cut(s) 272, 1431, 1947, 2010
BseCI ATCGAT 1 cut(s) 1869
BseDI CCNNGG 3 cut(s) 468, 881, 1429
BseGI GGATG 7 cut(s) 62, 393, 972, 1017, 1317, 1942, 2244
BseLI CCNNNNNNNGG 9 cut(s) 469, 670, 770, 793, 887, 1095, 1308, 2020, 2267
BseMI GCAATG 2 cut(s) 555, 1170
BseMII CTCAG 3 cut(s) 75, 1662, 2136
BseNI ACTGG 5 cut(s) 737, 1013, 1109, 1209, 2252
BseRI GAGGAG 1 cut(s) 587
BseSI GKGCMC 1 cut(s) 1251
BseXI GCAGC 5 cut(s) 660, 1162, 1493, 1552, 2263
BseYI CCCAGC 1 cut(s) 168
BsgI GTGCAG 2 cut(s) 407, 1743
Bsh1236I CGCG 1 cut(s) 359
BshVI ATCGAT 1 cut(s) 1869
BsiHKAI GWGCWC 5 cut(s) 485, 1251, 1300, 1555, 2035
BsiSI CCGG 3 cut(s) 1926, 2021, 2261
BslFI GGGAC 2 cut(s) 1432, 1744
BslI CCNNNNNNNGG 9 cut(s) 469, 670, 770, 793, 887, 1095, 1308, 2020, 2267
BsmAI GTCTC 4 cut(s) 524, 1285, 2083, 2141
BsmBI CGTCTC 1 cut(s) 1285
BsmFI GGGAC 2 cut(s) 1432, 1744
BsmI GAATGC 1 cut(s) 573
Bsp1286I GDGCHC 6 cut(s) 485, 1090, 1251, 1300, 1555, 2035
Bsp13I TCCGGA 1 cut(s) 2260
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 6 cut(s) 322, 659, 687, 918, 1850, 1858
Bsp19I CCATGG 1 cut(s) 468
BspACI CCGC 3 cut(s) 1535, 1743, 2291
BspCNI CTCAG 3 cut(s) 74, 1663, 2137
BspDI ATCGAT 1 cut(s) 1869
BspEI TCCGGA 1 cut(s) 2260
BspFNI CGCG 1 cut(s) 359
BspHI TCATGA 1 cut(s) 726
BspLI GGNNCC 3 cut(s) 324, 1087, 2271
BspMI ACCTGC 2 cut(s) 98, 159
BspPI GGATC 3 cut(s) 317, 330, 654
BspTI CTTAAG 1 cut(s) 1966
BsrDI GCAATG 2 cut(s) 555, 1170
BsrFI RCCGGY 1 cut(s) 1925
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 5 cut(s) 737, 1013, 1109, 1209, 2252
BssAI RCCGGY 1 cut(s) 1925
BssECI CCNNGG 3 cut(s) 468, 881, 1429
BssMI GATC 6 cut(s) 322, 659, 687, 918, 1850, 1858
BssT1I CCWWGG 2 cut(s) 468, 881
Bst2UI CCWGG 4 cut(s) 272, 1431, 1947, 2010
Bst6I CTCTTC 4 cut(s) 597, 886, 2037, 2197
BstAFI CTTAAG 1 cut(s) 1966
BstAPI GCANNNNNTGC 2 cut(s) 151, 262
BstAUI TGTACA 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 6 cut(s) 548, 820, 1251, 1284, 1623, 1646
BstDEI CTNAG 4 cut(s) 61, 1671, 2076, 2145
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 7 cut(s) 62, 393, 972, 1017, 1317, 1942, 2244
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 6 cut(s) 325, 662, 690, 921, 1853, 1861
BstMAI GTCTC 4 cut(s) 524, 1285, 2083, 2141
BstMBI GATC 6 cut(s) 322, 659, 687, 918, 1850, 1858
BstMWI GCNNNNNNNGC 8 cut(s) 151, 262, 365, 803, 863, 986, 1175, 1347
BstNI CCWGG 4 cut(s) 272, 1431, 1947, 2010
BstNSI RCATGY 5 cut(s) 92, 301, 1648, 2096, 2103
BstSCI CCNGG 5 cut(s) 270, 1429, 1945, 2008, 2019
BstSFI CTRYAG 2 cut(s) 561, 1044
BstSLI GKGCMC 1 cut(s) 1251
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 5 cut(s) 660, 1162, 1493, 1552, 2263
BstV2I GAAGAC 1 cut(s) 1596
BstX2I RGATCY 3 cut(s) 322, 659, 1850
BstYI RGATCY 3 cut(s) 322, 659, 1850
Bsu15I ATCGAT 1 cut(s) 1869
BsuTUI ATCGAT 1 cut(s) 1869
BtgI CCRYGG 1 cut(s) 468
BtsCI GGATG 7 cut(s) 62, 393, 972, 1017, 1317, 1942, 2244
BtsI GCAGTG 2 cut(s) 1560, 2261
BtsIMutI CAGTG 4 cut(s) 1239, 1560, 2261, 2347
BveI ACCTGC 2 cut(s) 98, 159
Cac8I GCNNGC 6 cut(s) 548, 820, 1251, 1284, 1623, 1646
CaiI CAGNNNCTG 1 cut(s) 1430
CciI TCATGA 1 cut(s) 726
Cfr10I RCCGGY 1 cut(s) 1925
Cfr13I GGNCC 6 cut(s) 472, 1370, 1471, 1687, 1777, 2270
ClaI ATCGAT 1 cut(s) 1869
CseI GACGC 2 cut(s) 365, 1986
CsiI ACCWGGT 1 cut(s) 270
Csp6I GTAC 8 cut(s) 65, 124, 497, 838, 936, 1212, 1386, 2182
CviQI GTAC 8 cut(s) 65, 124, 497, 838, 936, 1212, 1386, 2182
DdeI CTNAG 4 cut(s) 61, 1671, 2076, 2145
DpnI GATC 6 cut(s) 324, 661, 689, 920, 1852, 1860
DpnII GATC 6 cut(s) 322, 659, 687, 918, 1850, 1858
DraIII CACNNNGTG 1 cut(s) 1721
DrdI GACNNNNNNGTC 1 cut(s) 2381
DseDI GACNNNNNNGTC 1 cut(s) 2381
EaeI YGGCCR 2 cut(s) 1167, 2011
Eam1104I CTCTTC 4 cut(s) 597, 886, 2037, 2197
EarI CTCTTC 4 cut(s) 597, 886, 2037, 2197
Ecl136II GAGCTC 1 cut(s) 1553
Eco130I CCWWGG 2 cut(s) 468, 881
Eco24I GRGCYC 2 cut(s) 1090, 1555
Eco47I GGWCC 4 cut(s) 472, 1471, 1777, 2270
Eco53kI GAGCTC 1 cut(s) 1553
Eco57I CTGAAG 1 cut(s) 1304
EcoICRI GAGCTC 1 cut(s) 1553
EcoO109I RGGNCCY 1 cut(s) 1777
EcoRI GAATTC 2 cut(s) 992, 1806
EcoRII CCWGG 4 cut(s) 270, 1429, 1945, 2008
EcoT14I CCWWGG 2 cut(s) 468, 881
EcoT38I GRGCYC 2 cut(s) 1090, 1555
ErhI CCWWGG 2 cut(s) 468, 881
Esp3I CGTCTC 1 cut(s) 1285
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 2 cut(s) 1432, 1744
FauI CCCGC 1 cut(s) 2284
FauNDI CATATG 1 cut(s) 328
FbaI TGATCA 2 cut(s) 687, 1858
FblI GTMKAC 2 cut(s) 1815, 2005
Fnu4HI GCNGC 5 cut(s) 649, 1176, 1482, 1566, 2252
FokI GGATG 7 cut(s) 69, 380, 979, 1024, 1324, 1929, 2251
FriOI GRGCYC 2 cut(s) 1090, 1555
Fsp4HI GCNGC 5 cut(s) 649, 1176, 1482, 1566, 2252
FspBI CTAG 9 cut(s) 203, 308, 398, 516, 782, 1124, 1490, 1610, 2357
GluI GCNGC 5 cut(s) 649, 1176, 1482, 1566, 2252
GsaI CCCAGC 1 cut(s) 172
GsuI CTGGAG 2 cut(s) 1104, 1452
HapII CCGG 3 cut(s) 1926, 2021, 2261
HgaI GACGC 2 cut(s) 365, 1986
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 2 cut(s) 77, 978
HinfI GANTC 3 cut(s) 870, 1112, 1676
HpaI GTTAAC 1 cut(s) 739
HpaII CCGG 3 cut(s) 1926, 2021, 2261
HphI GGTGA 6 cut(s) 19, 708, 919, 1951, 2227, 2291
Hpy166II GTNNAC 7 cut(s) 739, 1249, 1510, 1768, 1816, 1981, 2006
Hpy188III TCNNGA 7 cut(s) 50, 727, 1004, 1083, 1630, 1848, 2261
Hpy8I GTNNAC 7 cut(s) 739, 1249, 1510, 1768, 1816, 1981, 2006
Hpy99I CGWCG 1 cut(s) 1684
HpyAV CCTTC 5 cut(s) 662, 749, 817, 881, 1373
HpyCH4IV ACGT 2 cut(s) 229, 2231
HpyF10VI GCNNNNNNNGC 8 cut(s) 151, 262, 365, 803, 863, 986, 1175, 1347
HpyF3I CTNAG 4 cut(s) 61, 1671, 2076, 2145
HpySE526I ACGT 2 cut(s) 229, 2231
Kpn2I TCCGGA 1 cut(s) 2260
Ksp22I TGATCA 2 cut(s) 687, 1858
KspAI GTTAAC 1 cut(s) 739
Kzo9I GATC 6 cut(s) 322, 659, 687, 918, 1850, 1858
LmnI GCTCC 3 cut(s) 1085, 1795, 1802
Lsp1109I GCAGC 5 cut(s) 660, 1162, 1493, 1552, 2263
LweI GCATC 3 cut(s) 47, 622, 2137
MabI ACCWGGT 1 cut(s) 270
MaeI CTAG 9 cut(s) 203, 308, 398, 516, 782, 1124, 1490, 1610, 2357
MaeII ACGT 2 cut(s) 229, 2231
MaeIII GTNAC 6 cut(s) 266, 1314, 1447, 1522, 1537, 1558
MalI GATC 6 cut(s) 324, 661, 689, 920, 1852, 1860
MboI GATC 6 cut(s) 322, 659, 687, 918, 1850, 1858
MflI RGATCY 3 cut(s) 322, 659, 1850
MhlI GDGCHC 6 cut(s) 485, 1090, 1251, 1300, 1555, 2035
MlsI TGGCCA 2 cut(s) 1169, 2013
MluCI AATT 8 cut(s) 68, 278, 992, 1272, 1569, 1806, 2137, 2300
MluNI TGGCCA 2 cut(s) 1169, 2013
MlyI GAGTC 1 cut(s) 1670
MmeI TCCRAC 1 cut(s) 1036
Mox20I TGGCCA 2 cut(s) 1169, 2013
MroI TCCGGA 1 cut(s) 2260
MscI TGGCCA 2 cut(s) 1169, 2013
MslI CAYNNNNRTG 3 cut(s) 302, 1244, 1947
Msp20I TGGCCA 2 cut(s) 1169, 2013
MspCI CTTAAG 1 cut(s) 1966
MspI CCGG 3 cut(s) 1926, 2021, 2261
MspR9I CCNGG 5 cut(s) 272, 1431, 1947, 2010, 2021
Mva1269I GAATGC 1 cut(s) 573
MvaI CCWGG 4 cut(s) 272, 1431, 1947, 2010
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 8 cut(s) 151, 262, 365, 803, 863, 986, 1175, 1347
NciI CCSGG 1 cut(s) 2021
NcoI CCATGG 1 cut(s) 468
NdeI CATATG 1 cut(s) 328
NdeII GATC 6 cut(s) 322, 659, 687, 918, 1850, 1858
NlaIV GGNNCC 3 cut(s) 324, 1087, 2271
NmuCI GTSAC 3 cut(s) 1314, 1522, 1558
NspI RCATGY 5 cut(s) 92, 301, 1648, 2096, 2103
OliI CACNNNNGTG 1 cut(s) 1244
PaeI GCATGC 1 cut(s) 1648
PagI TCATGA 1 cut(s) 726
PciI ACATGT 2 cut(s) 88, 2092
PctI GAATGC 1 cut(s) 573
PfeI GAWTC 2 cut(s) 870, 1112
PkrI GCNGC 5 cut(s) 650, 1177, 1483, 1567, 2253
PleI GAGTC 1 cut(s) 1670
PpsI GAGTC 1 cut(s) 1670
Ppu21I YACGTR 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 1777
PscI ACATGT 2 cut(s) 88, 2092
PsiI TTATAA 2 cut(s) 2154, 2192
Psp124BI GAGCTC 1 cut(s) 1555
Psp5II RGGWCCY 1 cut(s) 1777
Psp6I CCWGG 4 cut(s) 270, 1429, 1945, 2008
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 4 cut(s) 270, 1429, 1945, 2008
PspN4I GGNNCC 3 cut(s) 324, 1087, 2271
PspPI GGNCC 6 cut(s) 472, 1370, 1471, 1687, 1777, 2270
PspPPI RGGWCCY 1 cut(s) 1777
PstNI CAGNNNCTG 1 cut(s) 1430
PsuI RGATCY 3 cut(s) 322, 659, 1850
RsaI GTAC 8 cut(s) 66, 125, 498, 839, 937, 1213, 1387, 2183
RsaNI GTAC 8 cut(s) 65, 124, 497, 838, 936, 1212, 1386, 2182
RseI CAYNNNNRTG 3 cut(s) 302, 1244, 1947
SacI GAGCTC 1 cut(s) 1555
SatI GCNGC 5 cut(s) 649, 1176, 1482, 1566, 2252
Sau3AI GATC 6 cut(s) 322, 659, 687, 918, 1850, 1858
Sau96I GGNCC 6 cut(s) 472, 1370, 1471, 1687, 1777, 2270
ScaI AGTACT 2 cut(s) 1213, 2183
SchI GAGTC 1 cut(s) 1670
ScrFI CCNGG 5 cut(s) 272, 1431, 1947, 2010, 2021
SduI GDGCHC 6 cut(s) 485, 1090, 1251, 1300, 1555, 2035
SexAI ACCWGGT 1 cut(s) 270
SfaNI GCATC 3 cut(s) 47, 622, 2137
SfcI CTRYAG 2 cut(s) 561, 1044
SinI GGWCC 4 cut(s) 472, 1471, 1777, 2270
SmiMI CAYNNNNRTG 3 cut(s) 302, 1244, 1947
SmlI CTYRAG 4 cut(s) 665, 1336, 1554, 1966
SmoI CTYRAG 4 cut(s) 665, 1336, 1554, 1966
SpeI ACTAGT 3 cut(s) 202, 515, 1489
SphI GCATGC 1 cut(s) 1648
Sse9I AATT 8 cut(s) 68, 278, 992, 1272, 1569, 1806, 2137, 2300
SsiI CCGC 3 cut(s) 1535, 1743, 2291
SspMI CTAG 9 cut(s) 203, 308, 398, 516, 782, 1124, 1490, 1610, 2357
SstI GAGCTC 1 cut(s) 1555
StyD4I CCNGG 5 cut(s) 270, 1429, 1945, 2008, 2019
StyI CCWWGG 2 cut(s) 468, 881
TaiI ACGT 2 cut(s) 232, 2234
TaqI TCGA 4 cut(s) 479, 1631, 1679, 1869
TasI AATT 8 cut(s) 68, 278, 992, 1272, 1569, 1806, 2137, 2300
TatI WGTACW 5 cut(s) 64, 123, 496, 1211, 2181
TfiI GAWTC 2 cut(s) 870, 1112
TscAI CASTG 4 cut(s) 1246, 1567, 2261, 2347
TseFI GTSAC 3 cut(s) 1314, 1522, 1558
TseI GCWGC 5 cut(s) 648, 1175, 1481, 1565, 2251
Tsp45I GTSAC 3 cut(s) 1314, 1522, 1558
TspGWI ACGGA 1 cut(s) 1484
TspRI CASTG 4 cut(s) 1246, 1567, 2261, 2347
Vha464I CTTAAG 1 cut(s) 1966
VneI GTGCAC 1 cut(s) 1247
VpaK11BI GGWCC 4 cut(s) 472, 1471, 1777, 2270
XapI RAATTY 4 cut(s) 278, 992, 1806, 2300
XceI RCATGY 5 cut(s) 92, 301, 1648, 2096, 2103
XcmI CCANNNNNNNNNTGG 1 cut(s) 1216
XmiI GTMKAC 2 cut(s) 1815, 2005
XspI CTAG 9 cut(s) 203, 308, 398, 516, 782, 1124, 1490, 1610, 2357
ZrmI AGTACT 2 cut(s) 1213, 2183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.